Cucsat.G9117 (gene) Cucumber (B10) v3

Overview
NameCucsat.G9117
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionDiacylglycerol O-acyltransferase
Locationctg1658: 54011 .. 56989 (+)
RNA-Seq ExpressionCucsat.G9117
SyntenyCucsat.G9117
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTTCTTTCAATATTGAAATTATAAATTTACAATCACACCTTTTCTTGAGAAATAACTATATATATATTTTTTGTAAATGTGGTAGGAAAAAAGTTGAAATTTAATACAACTAAAAATATACAATATATAAAAGAGGACCACGTCTCTTCTCTCTCTATAACTTTCCTTTCTTTGCCTCTCTGCTACACTCTTCGACTTCTCCACTCTTCTGTCTTTGACCTCTGCTTATTAGCCATTTTCATGGAACGTCCATTTCATCTTCACCCTACTCTTCTCTCTCTCTTTCCTTATACCACCTAATCTCATCATTCCTTCAATACCCTTTTCCCCTCTCCATGAATTCCGACGAGCCACTCACTCCTGCCGGCCGTCTCTTCCTCCGTCCTGAAATCAACCAGATTATCCACTGTCTCGTTGGACTAAAGAACTCAATCGATGTCGATTCTGTCAAATCTCAAATCGCTGATTCCATCATGATTCAGCACCCTAGGTTTTCGAGTCTTCTCGTTCGTGACCGCAACGGCGTTGAGTATTGGCGGAGGACTTCAATCGAAGTCGACCGTCACGTCATCGTCGTTTCCGATCCTGTATCGGATGACGTTGGAGGAGTAAACGATGAGAAGGCTGCGAATGAATATTTGGCTGATTTAGCGATTTCTTCCTCCATGGATTACAGCAAACCTCTTTGGGAGATTCATTTGCTTTTGGCGCATAACTGTGCCGTCTTCCGAATCCATCACGCGCTCGGTGATGGAATCTCGCTGATGTCGTTGTTCTTGACGTGTTGCCGGCGGGCTGATGATCCGGATGCGTTGCCGACGATCGTTTCGGATTTGAAGGCGGTGAGGACTGGAAACAGAGGGCGGAGGAGTTGTGGGGAGATGATGTTGGAGTTTCTTTTAACGGTGTGGTTTAGTTTGTTGTTTGTGTTGGAGTTTATCGTGAGAGCTTTGTGGGTTTGTGACCGGAAAACGCCGATATCCGGTGGGGATGGGGTGGAGCTTTGGCCGAGGAAAGTGGCGACGGCTAAGTTCGCGTTGGAGGATATGAAAGCGGTGAAGAAAGGAGTTCCTAACGCGGTAAGTTGGACTTCTTAGCGTCACCTTTTCCCTAAACTAAATTTATTCATTTTCATACTCTAAAATAGAATTTTCTCTCGTAAATAGATATATATCCAAAAGCAATACAATTTTAACTAAAATGTTCAAAGGTAAACATTCATAAATAATAATGGAACCAACATTTCCCATAACTATAACATATATAATTATTTCCCCAGGGGGACCTATGTTCAAAATGAAGAAAGAAATAGATAAAAAAGAGGCAATCCTTTTTTTCTTTTCTTTATTATGTAATTTTTGTTCTTACTATTTTCTTTTAGCAAAATTTGTTTTGTTACAAAAAGGAGGGAGAACGTGTTGAAGTCATCAAAACCAAAGACACGTTTAGAAATAAGCTTTTTTTCTTCTATCCAAAAACAATTTTGGTTAGTTTCTAAATAAATGTGTGTAGCATTACAAAAGAATTATTGAAGAATTTATTTAAAAATGTAGAACGTAGCTAATTGTGATTGGATCAAACATTTATGATTGGTTGCAGACCATAAATGACGTTCTATTCAGCGTAATAGGAGCAGGGTTATCGAGGTACTTGGAGCATAGGCAACCCAAAGGTTAGTCTTTCCTTTACCACTCCCAACTATTTTATACTCCAATGTATTTTGTTTGGTTAAATAACATTTTTTGCTCTTCAAATTTTAATATTGTTCTTGCTTTTAAAGTTTTATCTCTAATAAATTTGATGGGCCTATTACTTACTTTTTGAAGTTAGGTTTAGTTGGGAGCGTCAAATTAATATTTCATTGACTTATTGACCCAACTTTAATTTGAATTTTTAGGACTTCTTTATCGCTTTTTAAACATTCTTTTTACTTTTAGGTTTGAAAGAAGGACTTCAATTAACTGGAGTAGCTATGGTTAATCTTAGAGAGCAACCGGGATTGCAGGTTTTATAGATTATTATTTTTAAATAAAAATTTTCTTTTTGTTAAGAATTTTCTATTATTATACTACATTGTCGTCAACTCTCTATATGTATGGTATAGGATTTATCTGATATGATGAAAGGAAACAAGGGATCACGATGGGGTAACAAGTTAGGAATTCTTCTCCTTCCTGTAAACTATTACACGAAAGCACTCGATCCTCTTCAATATGTCAAGAGAACGAAAAAGATGCTTGATCGGAAAAAACGGACCTTCGAAGCTCATTTTTCGTACGGAATTGGCAAGTTGGTAATGTCCTTTTTGGGACCAAAGGTAGGATTATAGAAATTTAAAAGGTTGTGTAGTTCTAAATTTCTCATTATTGTTTTTAGATGTTTAGTAAATTATTTGTACACAATGTGTTTGCAGGTAGCTTGCATCCTTAATTACAGGATTGTTTGCAATACAAGTTTCACTATCTCCAATGTGATTGGCCCAAGAGAAGAGATTACCATTGGAGGCAATCCCGTTACTTACATCAGAGTCACTTCAACAAGCTTATCCCATGTACTACCACCTCCCACCTCTCTTCTTACCTATTTCATAACAAAACTTCAAAATGATTTTATGGACAGATCTAATAGAAATATATTGGTTAGTAGAGAAACAGTATGTAATAGAAATGAATATGGTTTGATGCAGGCATTAACAATGCATATGATGAGCTATGCTGGAAGAGCTGAAATGCAAATTTTGGTGGCAAAAGACATCATCCCTGATCCTGAATTCCTTGCTGAATGCTTTGAAAATGCTTTACTTGAAATGAAAACAGCAGGAGCAACATTAACCACAAAATAGCATATACAATAACTTCCCAAATTTAATTTTCTACTTGTTTATACATTGTTTAATCAAGAAAAAGCCAATCGATTAGACTTGATGAGGTAACGGTGTTTGAAACTACTGTTGAAATAGTTAAAATTATATTTTTTTCCATATAATTCTAAACTTTGAAATACGATTTTAATAAATCAAAATTGAGAATTATTTTGGGTGTGTGTTGGAATTATCTGAAAGAGGTATGCCATCAGTTTTGTAAGGACAATTGTCACTACCAAAATAAGAAAGAAACAGGAAAACAAAGGACAGAAAGGGCATGGAGGTACAACTGTATAAACCACATATTTTGTCTACTTTAATTTCCTTTATGAAAAA

Coding sequence (CDS)

ATGAATTCCGACGAGCCACTCACTCCTGCCGGCCGTCTCTTCCTCCGTCCTGAAATCAACCAGATTATCCACTGTCTCGTTGGACTAAAGAACTCAATCGATGTCGATTCTGTCAAATCTCAAATCGCTGATTCCATCATGATTCAGCACCCTAGGTTTTCGAGTCTTCTCGTTCGTGACCGCAACGGCGTTGAGTATTGGCGGAGGACTTCAATCGAAGTCGACCGTCACGTCATCGTCGTTTCCGATCCTGTATCGGATGACGTTGGAGGAGTAAACGATGAGAAGGCTGCGAATGAATATTTGGCTGATTTAGCGATTTCTTCCTCCATGGATTACAGCAAACCTCTTTGGGAGATTCATTTGCTTTTGGCGCATAACTGTGCCGTCTTCCGAATCCATCACGCGCTCGGTGATGGAATCTCGCTGATGTCGTTGTTCTTGACGTGTTGCCGGCGGGCTGATGATCCGGATGCGTTGCCGACGATCGTTTCGGATTTGAAGGCGGTGAGGACTGGAAACAGAGGGCGGAGGAGTTGTGGGGAGATGATGTTGGAGTTTCTTTTAACGGTGTGGTTTAGTTTGTTGTTTGTGTTGGAGTTTATCGTGAGAGCTTTGTGGGTTTGTGACCGGAAAACGCCGATATCCGGTGGGGATGGGGTGGAGCTTTGGCCGAGGAAAGTGGCGACGGCTAAGTTCGCGTTGGAGGATATGAAAGCGGTGAAGAAAGGAGTTCCTAACGCGACCATAAATGACGTTCTATTCAGCGTAATAGGAGCAGGGTTATCGAGGTACTTGGAGCATAGGCAACCCAAAGGTTTGAAAGAAGGACTTCAATTAACTGGAGTAGCTATGGTTAATCTTAGAGAGCAACCGGGATTGCAGGATTTATCTGATATGATGAAAGGAAACAAGGGATCACGATGGGGTAACAAGTTAGGAATTCTTCTCCTTCCTGTAAACTATTACACGAAAGCACTCGATCCTCTTCAATATGTCAAGAGAACGAAAAAGATGCTTGATCGGAAAAAACGGACCTTCGAAGCTCATTTTTCGTACGGAATTGGCAAGTTGGTAATGTCCTTTTTGGGACCAAAGGTAGCTTGCATCCTTAATTACAGGATTGTTTGCAATACAAGTTTCACTATCTCCAATGTGATTGGCCCAAGAGAAGAGATTACCATTGGAGGCAATCCCGTTACTTACATCAGAGTCACTTCAACAAGCTTATCCCATGCATTAACAATGCATATGATGAGCTATGCTGGAAGAGCTGAAATGCAAATTTTGGTGGCAAAAGACATCATCCCTGATCCTGAATTCCTTGCTGAATGCTTTGAAAATGCTTTACTTGAAATGAAAACAGCAGGAGCAACATTAACCACAAAATAG

Protein sequence

MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEIHLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSCGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVMSFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMMSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK
Homology
BLAST of Cucsat.G9117 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 5.0e-58
Identity = 143/394 (36.29%), Postives = 221/394 (56.09%), Query Frame = 0

Query: 4   DEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRNG 63
           ++PL+PA RLF  PE N  I  +VGLKN I+ D +   I  ++M +HPRFSS LV + N 
Sbjct: 25  EQPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVNNCNN 84

Query: 64  ---VEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 123
               + W RT++ V+ HVI+     +  +   N +     Y++DL  +  +D SKPLWE+
Sbjct: 85  NRQEQKWVRTNVVVEDHVIIPKIQ-TQHIENANADVFLESYVSDLT-TIPLDTSKPLWEV 144

Query: 124 HLL-----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTI------VSDLKA 183
           HLL      A N AV RIHH+LGDG+S+MSL L C R+  +P+ LP++       S   +
Sbjct: 145 HLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSGSSS 204

Query: 184 VRTGNRGRRSCGEMMLEFLLTVWFSLLFV-------LEFIVRALWVCDRKTPISGGDGVE 243
           ++T +R    C       ++ +W + L V       LEFI  AL++ D +TPI G   + 
Sbjct: 205 LKTSSR----CYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIKGDFKLS 264

Query: 244 LWPRKVATAK-FALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEH---------RQPK 303
              R     +  +L+D+K +K  +   T+NDV+  V  AGLS+YL+          R   
Sbjct: 265 KGKRMCMVHRTVSLDDIKLIKNAM-KMTVNDVVLGVSQAGLSQYLKRRYGEQEESKRNSS 324

Query: 304 GLKEGLQLTGVAMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKAL--DPL 363
            + +G++L    +VNLR   G+QDL+DMM      RWGN +G ++ P   ++ AL  DPL
Sbjct: 325 NIPKGIRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFP---FSIALCDDPL 384

Query: 364 QYVKRTKKMLDRKKRTFEAHFSYGIGKLVMSFLG 365
           ++++R K  +DRKK + EA  ++ +GK++++ LG
Sbjct: 385 KHLRRAKSTIDRKKNSLEAVLTFVVGKILLNTLG 407

BLAST of Cucsat.G9117 vs. ExPASy Swiss-Prot
Match: Q5KS41 (Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=WSD11 PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.8e-55
Identity = 139/385 (36.10%), Postives = 204/385 (52.99%), Query Frame = 0

Query: 4   DEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRNG 63
           +EPL+P  RLF  P+ N  I   +G K   D  ++   + +  ++ HPRFSS+L  +   
Sbjct: 14  EEPLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGL-EHTLVNHPRFSSILEMNNGK 73

Query: 64  VEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEIHLL 123
              W RT ++V+ HVIV    V  D+   N ++   +Y++ L  +  MD SKPLWE+HLL
Sbjct: 74  KPRWVRTKVKVEEHVIV--PDVDPDI--ENPDQYLEDYISKLT-TIPMDLSKPLWEMHLL 133

Query: 124 -----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRR 183
                 A + A+ +IHH+LGDG+SLMSL L C R+  DP+ALPT+    K  R G     
Sbjct: 134 GVKTSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVHKK--RFGPSCNS 193

Query: 184 SCGEMMLEFLLTVWF--SLLF-----VLEFIVRALWVCDRKTPISGGDGVELWPRKVATA 243
                +    + +WF   LLF     +L F +    + D +TP+    G EL P++    
Sbjct: 194 GFFNKIWWLFVGLWFILRLLFNTFVDILMFALTIFVLRDTETPLLAKPGSELIPKRFVHR 253

Query: 244 KFALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEHR-------QPKGLKEGLQLTGVA 303
             + +D+K VK  +   T+NDVL  V  AGLSRYL  +       + K     ++L    
Sbjct: 254 IISFDDVKLVKNAM-KMTVNDVLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAI 313

Query: 304 MVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKK 363
           M+NLR   G++ L+DMM      RWGN  G +LLP +   +  DPL+YV++ K  +DRKK
Sbjct: 314 MINLRPNAGIEALADMMAKKSKCRWGNLFGYILLPFSVGLET-DPLEYVRQAKATIDRKK 373

Query: 364 RTFEAHFSYGIGKLVMSFLGPKVGL 370
            + EA FS    KL++  LG K  +
Sbjct: 374 HSLEAVFSMAFFKLILKVLGLKASV 388

BLAST of Cucsat.G9117 vs. ExPASy Swiss-Prot
Match: Q9M3B1 (Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=WSD6 PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 7.5e-54
Identity = 132/404 (32.67%), Postives = 218/404 (53.96%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           +  ++PL+PA R+F  PE N  +  ++G+K  ID D +   +  + +I+HPRFSS +V  
Sbjct: 22  VEEEQPLSPAARVFHAPEFNCYVISVIGIKKKIDPDVIIEGLKQT-LIRHPRFSSKMVST 81

Query: 61  RNG-----VEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSK 120
             G      + W RT++ V  HVI VSD  + ++   N +     Y+++L  +  +D SK
Sbjct: 82  SVGNKKRQTQSWVRTNVVVTDHVI-VSDIQTQNIENGNADAFLETYVSNLT-TVPLDISK 141

Query: 121 PLWEIHLL-----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAV 180
           PLW++HLL      A N AV + HH+LGDG+SLM+L L C R+  +PD LP++ +  ++ 
Sbjct: 142 PLWQLHLLDLKTSDAENVAVLKFHHSLGDGMSLMALVLACMRKTSNPDELPSLPNQNRSS 201

Query: 181 RTGNR---GRRSCGEMMLEFLLTVWFSLLFV-------LEFIVRALWVCDRKTPISGGDG 240
              +R   G R      L  ++ +W +++ V       LEFI   +++ D +TPI G   
Sbjct: 202 SRSSRLMAGSRG-DSRFLWLVMVIWSAIMLVLNTVCDALEFIATTMFLKDTETPIKGDFR 261

Query: 241 VELWPRKVATAK-FALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEH----------- 300
                R     +  +L+D+K +K  +   T+NDV+  V  AGLS+YL+            
Sbjct: 262 FSKSKRMCLVHRTVSLDDIKLIKNTM-KMTVNDVVLGVSQAGLSQYLDRRYGEKKKKVGE 321

Query: 301 -----RQPKGLKEGLQLTGVAMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYY 360
                R+   + + ++L    +VNLR   G+QDL+DMM      RWGN +G ++ P +  
Sbjct: 322 DQDSKRKATDMPKRIRLRSALLVNLRPNTGIQDLADMMAKGSTCRWGNWIGYIVFPFSIG 381

Query: 361 TKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVMSFLGPKV 368
            +  DPLQ+++R K+++DRKK + EA  ++  GK ++   G +V
Sbjct: 382 LRD-DPLQHLRRAKRIIDRKKNSLEAALTFVAGKFILKTFGVQV 419

BLAST of Cucsat.G9117 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.3e-53
Identity = 138/393 (35.11%), Postives = 214/393 (54.45%), Query Frame = 0

Query: 4   DEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRNG 63
           ++PL+PA RLF  PE N  I  ++G K+ +D         +S  I+HPRFSS LV D NG
Sbjct: 17  EQPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKES-FIRHPRFSSKLVTDENG 76

Query: 64  V-EYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEIHL 123
             + W RT++ V+ H IV   P        N      +Y++DL +   +D S+PLWE+HL
Sbjct: 77  QNQRWVRTNVVVEDHFIV---PKIKPQNIENSNAFLEDYVSDL-MKIPLDTSRPLWELHL 136

Query: 124 L-----LAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKA------VR 183
           L      A N AV +IHH++GDG+S+MSL L C R+  +PD LP++    ++      + 
Sbjct: 137 LDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLT 196

Query: 184 TGNRGRRSCGEMMLEFLLTVWFSLLF-------VLEFIVRALWVCDRKTPISGGD-GVEL 243
           TG+R        +L  +  +W +++         LEFIV  L+V D +TPI G     + 
Sbjct: 197 TGSRS----DSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIKGDFLSTKS 256

Query: 244 WPRKVATAKFALEDMKAVKKGVPNATINDVLFSVIGAGLSRYL---------EHRQPKGL 303
              ++     +L+D+K  K  + N TINDV+  V  AGLSRYL         ++R+ K L
Sbjct: 257 KQLRLVHRTVSLDDIKLTKNAM-NMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKL 316

Query: 304 KEGLQLTGVAMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVK 363
            + ++L    +VNLR   G+QDL+DMM+     RWGN  G ++ P +   +  DPL++++
Sbjct: 317 PKRIRLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFPFSIALRD-DPLEHLE 376

Query: 364 RTKKMLDRKKRTFEAHFSYGIGKLVMSFLGPKV 368
             +K + RKK ++ A  +Y   ++++  LG KV
Sbjct: 377 IAQKTISRKKNSYGAMLTYIFCRIIVKLLGIKV 398

BLAST of Cucsat.G9117 vs. ExPASy Swiss-Prot
Match: Q9C7H4 (Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=WSD2 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.6e-51
Identity = 137/381 (35.96%), Postives = 201/381 (52.76%), Query Frame = 0

Query: 3   SDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVR--- 62
           ++EP++P  RLF  P ++      +G K   +  ++   I ++ +I HPRFSS+LV    
Sbjct: 10  AEEPVSPFARLFSLPGLDVFNIVTIGCKTEGNASTIVEGIKNT-LINHPRFSSILVTGHG 69

Query: 63  DRNGVEYWRRTSIEVDRHVIVVS-DPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLW 122
           +  G   W  T I V+ HVIV   DP  +     N ++   +Y +++A+ S MD SKPLW
Sbjct: 70  EHKGKARWIPTKINVEEHVIVPDIDPNIE-----NPDEFLEDYTSNMAL-SPMDMSKPLW 129

Query: 123 EIHLLL-----AHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTG 182
           E HLL      A    V R HH+LGDG+SLMSL L C R+  DP+A PT V+  K     
Sbjct: 130 EFHLLKLKTSHAEAVTVARFHHSLGDGMSLMSLLLACTRKTCDPEAFPTFVAPKK----- 189

Query: 183 NRGRRSCGEMM--LEFLLTVWF-SLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVAT 242
           N+ +  C  ++  L F++ + F + + V++ IV      D    I G  G  L   K   
Sbjct: 190 NKAKNVCFSLVAWLWFIVRLMFHTCVEVIKSIVFICRASDTSAHIMGKPGATLSANKFIH 249

Query: 243 AKFALEDMKAVKKGVPNATINDVLFSVIGAGLSRYLEHR-----QPKGLK--EGLQLTGV 302
              +L+D+K VK  + N T+NDVLF ++ AGLSRYL  R       K  K    + L GV
Sbjct: 250 RIISLDDVKMVKNAM-NMTVNDVLFGMVQAGLSRYLNQRYDLETSSKSRKNLHNIGLHGV 309

Query: 303 AMVNLREQPGLQDLSDMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRK 362
              NLR    ++DL+ MM      RWGN +G +L+P+    +  D  +YV++ K ++D K
Sbjct: 310 VFFNLRPNRNIEDLAKMMAKGSKCRWGNSIGYVLIPLGMKPQD-DVFEYVRQAKTIMDGK 369

Query: 363 KRTFEAHFSYGIGKLVMSFLG 365
           K + E  FSYG+ K+ M   G
Sbjct: 370 KHSLEPLFSYGLLKVTMEVFG 376

BLAST of Cucsat.G9117 vs. NCBI nr
Match: XP_004136970.1 (O-acyltransferase WSD1 [Cucumis sativus] >KGN43919.1 hypothetical protein Csa_017278 [Cucumis sativus])

HSP 1 Score: 738 bits (1906), Expect = 2.99e-267
Identity = 367/367 (100.00%), Postives = 367/367 (100.00%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD
Sbjct: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
           RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI
Sbjct: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC 180
           HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC
Sbjct: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC 180

Query: 181 GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA 240
           GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA
Sbjct: 181 GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA 240

Query: 241 VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM 300
           VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM
Sbjct: 241 VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM 300

Query: 301 MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM 360
           MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM
Sbjct: 301 MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM 360

Query: 361 SFLGPKV 367
           SFLGPKV
Sbjct: 361 SFLGPKV 367

BLAST of Cucsat.G9117 vs. NCBI nr
Match: XP_008454962.1 (PREDICTED: O-acyltransferase WSD1-like isoform X1 [Cucumis melo] >KAA0031307.1 O-acyltransferase WSD1-like isoform X1 [Cucumis melo var. makuwa] >TYK06759.1 O-acyltransferase WSD1-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 700 bits (1806), Expect = 5.09e-252
Identity = 353/368 (95.92%), Postives = 359/368 (97.55%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           MNSDEPLTPAGRLFLRPEINQIIHCLVG++NSIDVDSVKSQIADSIMIQHPRFSSLLVRD
Sbjct: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGVQNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
           RNGVEYWRRTSIEVDRHVIVVSD VSDDVG VNDEKAANEYLADLAISSSMDYSKPLWEI
Sbjct: 61  RNGVEYWRRTSIEVDRHVIVVSDRVSDDVG-VNDEKAANEYLADLAISSSMDYSKPLWEI 120

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR-TGNRGRRS 180
           HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR T NRGRRS
Sbjct: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRSTRNRGRRS 180

Query: 181 CGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMK 240
            GEMMLEFLL VWFSLLFVLEFI RALWVCDRKTPISGG+GVELWPRKVATAKFALEDMK
Sbjct: 181 LGEMMLEFLLAVWFSLLFVLEFIGRALWVCDRKTPISGGEGVELWPRKVATAKFALEDMK 240

Query: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSD 300
           AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDL+D
Sbjct: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLAD 300

Query: 301 MMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLV 360
           MMKGN+GSRWGNKLGILLLPVNYY K LDPLQYV+RTKKMLDRKKRTFEAHFSYGIGKLV
Sbjct: 301 MMKGNRGSRWGNKLGILLLPVNYYKKTLDPLQYVERTKKMLDRKKRTFEAHFSYGIGKLV 360

Query: 361 MSFLGPKV 367
           MSFLGPKV
Sbjct: 361 MSFLGPKV 367

BLAST of Cucsat.G9117 vs. NCBI nr
Match: XP_038888558.1 (O-acyltransferase WSD1-like [Benincasa hispida])

HSP 1 Score: 625 bits (1613), Expect = 3.62e-222
Identity = 312/368 (84.78%), Postives = 337/368 (91.58%), Query Frame = 0

Query: 3   SDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRN 62
           +DEPLTPAGRLFLRPEINQIIHC+VGL+NSID+DSVKSQIADS+MIQHPRFSSLLVRDRN
Sbjct: 32  ADEPLTPAGRLFLRPEINQIIHCVVGLQNSIDIDSVKSQIADSVMIQHPRFSSLLVRDRN 91

Query: 63  GVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEIHL 122
           GVEYWRRTSIE+DRHVIVV++ VSD+  GV+DEKA N+YLADLAIS SMD  KPLWEIHL
Sbjct: 92  GVEYWRRTSIEIDRHVIVVAERVSDE--GVDDEKAVNQYLADLAISPSMDADKPLWEIHL 151

Query: 123 LLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSCGE 182
           LLAHNCAVFRIHHALGDGISLMS+FLTCCRRADDP+ALPTIVS+ KAVR   R R    E
Sbjct: 152 LLAHNCAVFRIHHALGDGISLMSMFLTCCRRADDPNALPTIVSNSKAVRRNMRQRSWADE 211

Query: 183 ---MMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMK 242
              M+LEFL  VWFS  FV EFI+RALWV DRKTPISGGDGVELWPRKVATAKFA+EDMK
Sbjct: 212 IPKMLLEFLFAVWFSFWFVAEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVEDMK 271

Query: 243 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSD 302
           AVKK VPNATIND+LFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDL++
Sbjct: 272 AVKKAVPNATINDILFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLTN 331

Query: 303 MMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLV 362
           MMKGN+GSRWGNKLG+LLLPVN + K LDPLQYVK+TKK+LDRKKRT EAHFSYGIGKLV
Sbjct: 332 MMKGNRGSRWGNKLGVLLLPVNCHKKMLDPLQYVKKTKKLLDRKKRTLEAHFSYGIGKLV 391

Query: 363 MSFLGPKV 367
           MS LGPKV
Sbjct: 392 MSCLGPKV 397

BLAST of Cucsat.G9117 vs. NCBI nr
Match: XP_022988766.1 (O-acyltransferase WSD1-like [Cucurbita maxima])

HSP 1 Score: 605 bits (1559), Expect = 2.51e-214
Identity = 299/369 (81.03%), Postives = 328/369 (88.89%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           + SD+PLTPAGRLFLRPEINQIIHC++GL+NSIDVDS+KSQIADS+MIQHPRFSSLLVRD
Sbjct: 7   LESDQPLTPAGRLFLRPEINQIIHCVIGLRNSIDVDSIKSQIADSVMIQHPRFSSLLVRD 66

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
            NGVEYWRRTSIEVDRHVIVVSDPVSDD  G ++E A N+YLADL+ SS+MD +KPLWEI
Sbjct: 67  PNGVEYWRRTSIEVDRHVIVVSDPVSDD--GASNENAVNDYLADLSTSSTMDINKPLWEI 126

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGR--R 180
           HLLLAHNC +FRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSD K  R   + R   
Sbjct: 127 HLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDSKTARKARQKRWAE 186

Query: 181 SCGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDM 240
              +M+L  L+TVWFS LFV EFI+RALWV DRKTPISGGDGVELWPRKVATAKFA+EDM
Sbjct: 187 ELPKMLLALLMTVWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVEDM 246

Query: 241 KAVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLS 300
           KAVK  V NATINDVLFSVIGAGLSRYLEHRQP  LKEGLQLTGVAM+NLREQPGLQDL+
Sbjct: 247 KAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQDLA 306

Query: 301 DMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKL 360
           DMMKGN GSRWGNKLGILLLPV Y+ K  DPLQY+KRTKKM+DRKKR+ E++FSYGIGK 
Sbjct: 307 DMMKGNNGSRWGNKLGILLLPVYYHKKMSDPLQYMKRTKKMIDRKKRSLESYFSYGIGKF 366

Query: 361 VMSFLGPKV 367
           VMS+LG KV
Sbjct: 367 VMSWLGAKV 373

BLAST of Cucsat.G9117 vs. NCBI nr
Match: XP_022927903.1 (O-acyltransferase WSD1-like [Cucurbita moschata])

HSP 1 Score: 605 bits (1559), Expect = 2.51e-214
Identity = 298/369 (80.76%), Postives = 328/369 (88.89%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           + SD+PLTPAGRLFLRPEINQIIHC++GL+NSIDVDS+KSQIADS+MIQHPRFSSLLVRD
Sbjct: 7   LESDQPLTPAGRLFLRPEINQIIHCVIGLQNSIDVDSIKSQIADSVMIQHPRFSSLLVRD 66

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
            NGVEYWRRTSIEVDRHVIVVSDPVSDD  G  +E A N+YLADL+ SS+MD +KPLWEI
Sbjct: 67  PNGVEYWRRTSIEVDRHVIVVSDPVSDD--GTGNENAVNDYLADLSTSSTMDMNKPLWEI 126

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGR--R 180
           HLLLAHNC +FRIHHALGDGISLMSLFLTCCRRADDP+ALPTIVSD K  R   R R   
Sbjct: 127 HLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPNALPTIVSDSKTARKSRRQRWAE 186

Query: 181 SCGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDM 240
              +M+LE L+T+WFS LFV EFI+RALWV DRKTPISGGDGVELWPRKVATAKFA+EDM
Sbjct: 187 ELPKMLLELLMTLWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVEDM 246

Query: 241 KAVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLS 300
           KAVK  V NATINDVLFSVIGAGLSRYLEHRQP  LKEGLQLTGVAM+NLREQPGLQDL+
Sbjct: 247 KAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQDLA 306

Query: 301 DMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKL 360
           DMMKGN GSRWGNKLGILLLP+ Y+ K  DPLQY+KRTKKM+DRKKR+ E++FSYGIGK 
Sbjct: 307 DMMKGNNGSRWGNKLGILLLPIYYHKKTSDPLQYLKRTKKMIDRKKRSLESYFSYGIGKF 366

Query: 361 VMSFLGPKV 367
           VMS+LG KV
Sbjct: 367 VMSWLGAKV 373

BLAST of Cucsat.G9117 vs. ExPASy TrEMBL
Match: A0A0A0K4Q2 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G073550 PE=4 SV=1)

HSP 1 Score: 738 bits (1906), Expect = 1.45e-267
Identity = 367/367 (100.00%), Postives = 367/367 (100.00%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD
Sbjct: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
           RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI
Sbjct: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC 180
           HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC
Sbjct: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGRRSC 180

Query: 181 GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA 240
           GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA
Sbjct: 181 GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA 240

Query: 241 VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM 300
           VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM
Sbjct: 241 VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM 300

Query: 301 MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM 360
           MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM
Sbjct: 301 MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM 360

Query: 361 SFLGPKV 367
           SFLGPKV
Sbjct: 361 SFLGPKV 367

BLAST of Cucsat.G9117 vs. ExPASy TrEMBL
Match: A0A5D3C8S5 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00600 PE=4 SV=1)

HSP 1 Score: 700 bits (1806), Expect = 2.47e-252
Identity = 353/368 (95.92%), Postives = 359/368 (97.55%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           MNSDEPLTPAGRLFLRPEINQIIHCLVG++NSIDVDSVKSQIADSIMIQHPRFSSLLVRD
Sbjct: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGVQNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
           RNGVEYWRRTSIEVDRHVIVVSD VSDDVG VNDEKAANEYLADLAISSSMDYSKPLWEI
Sbjct: 61  RNGVEYWRRTSIEVDRHVIVVSDRVSDDVG-VNDEKAANEYLADLAISSSMDYSKPLWEI 120

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR-TGNRGRRS 180
           HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR T NRGRRS
Sbjct: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRSTRNRGRRS 180

Query: 181 CGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMK 240
            GEMMLEFLL VWFSLLFVLEFI RALWVCDRKTPISGG+GVELWPRKVATAKFALEDMK
Sbjct: 181 LGEMMLEFLLAVWFSLLFVLEFIGRALWVCDRKTPISGGEGVELWPRKVATAKFALEDMK 240

Query: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSD 300
           AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDL+D
Sbjct: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLAD 300

Query: 301 MMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLV 360
           MMKGN+GSRWGNKLGILLLPVNYY K LDPLQYV+RTKKMLDRKKRTFEAHFSYGIGKLV
Sbjct: 301 MMKGNRGSRWGNKLGILLLPVNYYKKTLDPLQYVERTKKMLDRKKRTFEAHFSYGIGKLV 360

Query: 361 MSFLGPKV 367
           MSFLGPKV
Sbjct: 361 MSFLGPKV 367

BLAST of Cucsat.G9117 vs. ExPASy TrEMBL
Match: A0A1S3BZB1 (Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495247 PE=4 SV=1)

HSP 1 Score: 700 bits (1806), Expect = 2.47e-252
Identity = 353/368 (95.92%), Postives = 359/368 (97.55%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           MNSDEPLTPAGRLFLRPEINQIIHCLVG++NSIDVDSVKSQIADSIMIQHPRFSSLLVRD
Sbjct: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGVQNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
           RNGVEYWRRTSIEVDRHVIVVSD VSDDVG VNDEKAANEYLADLAISSSMDYSKPLWEI
Sbjct: 61  RNGVEYWRRTSIEVDRHVIVVSDRVSDDVG-VNDEKAANEYLADLAISSSMDYSKPLWEI 120

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR-TGNRGRRS 180
           HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVR T NRGRRS
Sbjct: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRSTRNRGRRS 180

Query: 181 CGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMK 240
            GEMMLEFLL VWFSLLFVLEFI RALWVCDRKTPISGG+GVELWPRKVATAKFALEDMK
Sbjct: 181 LGEMMLEFLLAVWFSLLFVLEFIGRALWVCDRKTPISGGEGVELWPRKVATAKFALEDMK 240

Query: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSD 300
           AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDL+D
Sbjct: 241 AVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLAD 300

Query: 301 MMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLV 360
           MMKGN+GSRWGNKLGILLLPVNYY K LDPLQYV+RTKKMLDRKKRTFEAHFSYGIGKLV
Sbjct: 301 MMKGNRGSRWGNKLGILLLPVNYYKKTLDPLQYVERTKKMLDRKKRTFEAHFSYGIGKLV 360

Query: 361 MSFLGPKV 367
           MSFLGPKV
Sbjct: 361 MSFLGPKV 367

BLAST of Cucsat.G9117 vs. ExPASy TrEMBL
Match: A0A6J1JI63 (Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111486010 PE=4 SV=1)

HSP 1 Score: 605 bits (1559), Expect = 1.21e-214
Identity = 299/369 (81.03%), Postives = 328/369 (88.89%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           + SD+PLTPAGRLFLRPEINQIIHC++GL+NSIDVDS+KSQIADS+MIQHPRFSSLLVRD
Sbjct: 7   LESDQPLTPAGRLFLRPEINQIIHCVIGLRNSIDVDSIKSQIADSVMIQHPRFSSLLVRD 66

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
            NGVEYWRRTSIEVDRHVIVVSDPVSDD  G ++E A N+YLADL+ SS+MD +KPLWEI
Sbjct: 67  PNGVEYWRRTSIEVDRHVIVVSDPVSDD--GASNENAVNDYLADLSTSSTMDINKPLWEI 126

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGR--R 180
           HLLLAHNC +FRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSD K  R   + R   
Sbjct: 127 HLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDSKTARKARQKRWAE 186

Query: 181 SCGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDM 240
              +M+L  L+TVWFS LFV EFI+RALWV DRKTPISGGDGVELWPRKVATAKFA+EDM
Sbjct: 187 ELPKMLLALLMTVWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVEDM 246

Query: 241 KAVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLS 300
           KAVK  V NATINDVLFSVIGAGLSRYLEHRQP  LKEGLQLTGVAM+NLREQPGLQDL+
Sbjct: 247 KAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQDLA 306

Query: 301 DMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKL 360
           DMMKGN GSRWGNKLGILLLPV Y+ K  DPLQY+KRTKKM+DRKKR+ E++FSYGIGK 
Sbjct: 307 DMMKGNNGSRWGNKLGILLLPVYYHKKMSDPLQYMKRTKKMIDRKKRSLESYFSYGIGKF 366

Query: 361 VMSFLGPKV 367
           VMS+LG KV
Sbjct: 367 VMSWLGAKV 373

BLAST of Cucsat.G9117 vs. ExPASy TrEMBL
Match: A0A6J1EMC4 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434667 PE=4 SV=1)

HSP 1 Score: 605 bits (1559), Expect = 1.21e-214
Identity = 298/369 (80.76%), Postives = 328/369 (88.89%), Query Frame = 0

Query: 1   MNSDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRD 60
           + SD+PLTPAGRLFLRPEINQIIHC++GL+NSIDVDS+KSQIADS+MIQHPRFSSLLVRD
Sbjct: 7   LESDQPLTPAGRLFLRPEINQIIHCVIGLQNSIDVDSIKSQIADSVMIQHPRFSSLLVRD 66

Query: 61  RNGVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEI 120
            NGVEYWRRTSIEVDRHVIVVSDPVSDD  G  +E A N+YLADL+ SS+MD +KPLWEI
Sbjct: 67  PNGVEYWRRTSIEVDRHVIVVSDPVSDD--GTGNENAVNDYLADLSTSSTMDMNKPLWEI 126

Query: 121 HLLLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRTGNRGR--R 180
           HLLLAHNC +FRIHHALGDGISLMSLFLTCCRRADDP+ALPTIVSD K  R   R R   
Sbjct: 127 HLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPNALPTIVSDSKTARKSRRQRWAE 186

Query: 181 SCGEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDM 240
              +M+LE L+T+WFS LFV EFI+RALWV DRKTPISGGDGVELWPRKVATAKFA+EDM
Sbjct: 187 ELPKMLLELLMTLWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVEDM 246

Query: 241 KAVKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLS 300
           KAVK  V NATINDVLFSVIGAGLSRYLEHRQP  LKEGLQLTGVAM+NLREQPGLQDL+
Sbjct: 247 KAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQDLA 306

Query: 301 DMMKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKL 360
           DMMKGN GSRWGNKLGILLLP+ Y+ K  DPLQY+KRTKKM+DRKKR+ E++FSYGIGK 
Sbjct: 307 DMMKGNNGSRWGNKLGILLLPIYYHKKTSDPLQYLKRTKKMIDRKKRSLESYFSYGIGKF 366

Query: 361 VMSFLGPKV 367
           VMS+LG KV
Sbjct: 367 VMSWLGAKV 373

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3B25.0e-5836.29Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3... [more]
Q5KS411.8e-5536.10Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=... [more]
Q9M3B17.5e-5432.67Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B31.3e-5335.11Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3... [more]
Q9C7H41.6e-5135.96Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
XP_004136970.12.99e-267100.00O-acyltransferase WSD1 [Cucumis sativus] >KGN43919.1 hypothetical protein Csa_01... [more]
XP_008454962.15.09e-25295.92PREDICTED: O-acyltransferase WSD1-like isoform X1 [Cucumis melo] >KAA0031307.1 O... [more]
XP_038888558.13.62e-22284.78O-acyltransferase WSD1-like [Benincasa hispida][more]
XP_022988766.12.51e-21481.03O-acyltransferase WSD1-like [Cucurbita maxima][more]
XP_022927903.12.51e-21480.76O-acyltransferase WSD1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0K4Q21.45e-267100.00Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G073550 PE=4... [more]
A0A5D3C8S52.47e-25295.92Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BZB12.47e-25295.92Diacylglycerol O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103495247 PE=4 SV... [more]
A0A6J1JI631.21e-21481.03Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111486010 PE=... [more]
A0A6J1EMC41.21e-21480.76Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434667 P... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009721O-acyltransferase WSD1, C-terminalPFAMPF06974WS_DGAT_Ccoord: 309..411
e-value: 2.2E-26
score: 92.5
IPR004255O-acyltransferase, WSD1, N-terminalPFAMPF03007WES_acyltransfcoord: 108..253
e-value: 1.4E-9
score: 38.1
NoneNo IPR availablePANTHERPTHR31650:SF41O-ACYLTRANSFERASE WSD1-LIKE ISOFORM X1coord: 2..414
NoneNo IPR availableSUPERFAMILY52777CoA-dependent acyltransferasescoord: 6..150
IPR045034O-acyltransferase WSD1-likePANTHERPTHR31650O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEINcoord: 2..414

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G9117.T1Cucsat.G9117.T1mRNA
Cucsat.G9117.T2Cucsat.G9117.T2mRNA
Cucsat.G9117.T3Cucsat.G9117.T3mRNA
Cucsat.G9117.T6Cucsat.G9117.T6mRNA
Cucsat.G9117.T4Cucsat.G9117.T4mRNA
Cucsat.G9117.T5Cucsat.G9117.T5mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019432 triglyceride biosynthetic process
biological_process GO:0045017 glycerolipid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004144 diacylglycerol O-acyltransferase activity
molecular_function GO:0047196 long-chain-alcohol O-fatty-acyltransferase activity
molecular_function GO:0008374 O-acyltransferase activity