Cucsat.G8361 (gene) Cucumber (B10) v3

Overview
NameCucsat.G8361
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionProtein-serine/threonine phosphatase
Locationctg1557: 341842 .. 344191 (-)
RNA-Seq ExpressionCucsat.G8361
SyntenyCucsat.G8361
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCTTGTTGCGCTGCTCCAATATTATTGGGCCACGTTTCCACCACCTCCCCAAACTAATTCCATACGTGTCTTCTTCCGATCAAACCACTCTTTCTCAATCAACGGCTCTCATTTCCCTTTCCACTACGTCACGCACTTTACCACCTCCTTCCATCTTTCTCACTCTCTCTTCCCCTAATCTCTATTTTTCCTATTTCCCCTTCTTTTCTATCTCACCTTCCTTTTTAAACCATCCTCCCTCCGCCGGAACCACTCTTCTCTTTTCATCTGCTTTCCCTTCGTTGTTGTTTTTTTTTTTTTTTCTTTTTTAATTTCAATTCCGTGGCCGGCTTTAAATTTAATCAATTGCACTGCGGCTGAGGCTAGGCAAGGGAAGGGAGGGGAGATTTGTTGAATTAGTAATGGAGGAGATTTGTTGTAAAGTTATGAGTGAGAGTGAGGCGTCGTCTTCTTGTGAGCCGAGCTCGCGAGTGGCTAGACGTCGGAGAATGGAGATCAGGAGATGTAAATTTGTTACCAGGGTTGATCCGTCCGAGACGGAGACTGTTTCGAAACGGCAGAAGCTGGAGGATCGAGCTACGTCCATTTCTAGAGATTGTGATAACGCGGTTCAGAATTGTGATTCGGGAGAAGAAGAAACCGTTGGGAGATTTGTGGTTGGAGAGGTTTCGACCAAGGAGGTTTCGATTCCTCTGTCTAGGGAATCTTCTTGTGGGGTAGTGGTTCCGAAATTTGGATTCTCTTCTGTTTGTGGAAGGAGGAGAGAGATGGAGGATGCGGTGGCGGTTCATCCATCGCTCTGTTATACGGAGAAGAGAGCTAGTGATATGTTGCACTTCTTCGGTGTTTACGACGGCCATGGCTGTTCTCATGTAAGATCTCTAATTACTTAATTATTCTCTTGAACTGTAGTTAATTAGCTTTAATTTCTGTTTGATTAGTAGAAGAAACAAAAGGAATTGCAACTTGGTAGTAAAATAATTAGGTCATTGACTTTCTTCTTTTACGGCGAATTTTTGGGGATATTTACGGGAGGTTTTTCCGGTGTGTTAGGTGGCGATGAGGTGTAAGGAGCGGCTGCATGAACTGGTAAAAGATGAGCTAGATAAGGATGAGAAGGAAGACGCCGCCGGTGCGGCGGAGACAGAGGCGGAGACGGCGTCGCGGTGGGACCGTACTATGAAGAGAATCTTCTGGAGAATGGACAACGAAGTTGTGGCGCGAAACAATGAAGAAGTGGTTGCGAACTGTAGATGCGAACTACAGTCGCCGGATTGCGATGCCGTTGGATCTACCGCCGTTGTCGCAATTGTGACGCCGGATAAGATCATCGTGGCCAACTGCGGCGATTCCAGAGCCGTTCTCTGCCGTAACGGAAAGGCCATCCCTCTCTCCTCCGATCACAAGGTTAGTACTCAGATTTAAGTGCTAATTAATTGATTAATCCTCCGATTTTCAATTTCCAATAATCAACTGTTTTAAAATCTTACAGCCGGATCGTCCCGATGAACTTAGCCGGATCGAGGAAGCCGGCGGACGAGTGATTTACTGGGACGGACCGAGAGTCCTTGGAGTTCTCGCCATGTCAAGAGCCATAGGTAAGCAAAAATCAAATCAACGAGGCGCTGACTTTAACCCACCGAACAACAAAACTCTAACGATGAATTTGATTTATTTCAGGCGATAATTATCTGAAACCGTATGTGATATCGGAACCAGAGGTGACGATAACGAATCGTACGGCGGAGGATGAGTGTTTGATATTAGGGAGCGATGGACTATGGGATGTGGTGCCGAATGAGACGGCGTGTGGAGTGGCAAGCATGTGCTTGAGAGGGAAGGCGGAGGAGAGGTCGCCGGTGTCGCCGTCGAGTGAGGCGGAGACAGCAGCAGAAGGGGAAGAGAGAGGAAACGCCGACAAGGCCTGCAACGATGCGTCGATGCTGCTGACGAAGCTGGCGTTGGCTAGGCACAGTACGGACAACGTGAGCGTTGTTGTGGTGGACCTTAAGAGGGACGCTTACCCTTAGTATCTCTTTTTCTTTTCTTTTCTTTTCTTTTTTTCCCCATTTAGTACAAAGAATTTAATTTGAATATTTCAGATTAAAAGAAGTTTTTTTTTTCTTCTTCTAGAAATTAGAAAAGGGAAAATGGAGTTTTATTTTAGGTAATTTCTCTTCTTGAGAATCTTTTTATCTTTTTGAAAAGAAAAAACAAACAAAACAAAAGTGTAATTAAAAAGTTATCAATATCAATTATCAATTATCAATGTCAATGCATTCTCATGGATTTCTCAAAATTTTCATATAAAATTATACATCAAATATCTTATCGTTATCGTTATTGT

Coding sequence (CDS)

ATGGAGGAGATTTGTTGTAAAGTTATGAGTGAGAGTGAGGCGTCGTCTTCTTGTGAGCCGAGCTCGCGAGTGGCTAGACGTCGGAGAATGGAGATCAGGAGATGTAAATTTGTTACCAGGGTTGATCCGTCCGAGACGGAGACTGTTTCGAAACGGCAGAAGCTGGAGGATCGAGCTACGTCCATTTCTAGAGATTGTGATAACGCGGTTCAGAATTGTGATTCGGGAGAAGAAGAAACCGTTGGGAGATTTGTGGTTGGAGAGGTTTCGACCAAGGAGGTTTCGATTCCTCTGTCTAGGGAATCTTCTTGTGGGGTAGTGGTTCCGAAATTTGGATTCTCTTCTGTTTGTGGAAGGAGGAGAGAGATGGAGGATGCGGTGGCGGTTCATCCATCGCTCTGTTATACGGAGAAGAGAGCTAGTGATATGTTGCACTTCTTCGGTGTTTACGACGGCCATGGCTGTTCTCATGTGGCGATGAGGTGTAAGGAGCGGCTGCATGAACTGGTAAAAGATGAGCTAGATAAGGATGAGAAGGAAGACGCCGCCGGTGCGGCGGAGACAGAGGCGGAGACGGCGTCGCGGTGGGACCGTACTATGAAGAGAATCTTCTGGAGAATGGACAACGAAGTTGTGGCGCGAAACAATGAAGAAGTGGTTGCGAACTGTAGATGCGAACTACAGTCGCCGGATTGCGATGCCGTTGGATCTACCGCCGTTGTCGCAATTGTGACGCCGGATAAGATCATCGTGGCCAACTGCGGCGATTCCAGAGCCGTTCTCTGCCGTAACGGAAAGGCCATCCCTCTCTCCTCCGATCACAAGCCGGATCGTCCCGATGAACTTAGCCGGATCGAGGAAGCCGGCGGACGAGTGATTTACTGGGACGGACCGAGAGTCCTTGGAGTTCTCGCCATGTCAAGAGCCATAGGCGATAATTATCTGAAACCGTATGTGATATCGGAACCAGAGGTGACGATAACGAATCGTACGGCGGAGGATGAGTGTTTGATATTAGGGAGCGATGGACTATGGGATGTGGTGCCGAATGAGACGGCGTGTGGAGTGGCAAGCATGTGCTTGAGAGGGAAGGCGGAGGAGAGGTCGCCGGTGTCGCCGTCGAGTGAGGCGGAGACAGCAGCAGAAGGGGAAGAGAGAGGAAACGCCGACAAGGCCTGCAACGATGCGTCGATGCTGCTGACGAAGCTGGCGTTGGCTAGGCACAGTACGGACAACGTGAGCGTTGTTGTGGTGGACCTTAAGAGGGACGCTTACCCTTAG

Protein sequence

MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRATSISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRRREMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKEDAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTAVVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPRVLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASMCLRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVDLKRDAYP
Homology
BLAST of Cucsat.G8361 vs. ExPASy Swiss-Prot
Match: Q9FIF5 (Probable protein phosphatase 2C 78 OS=Arabidopsis thaliana OX=3702 GN=SAG113 PE=1 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 9.7e-114
Identity = 237/410 (57.80%), Postives = 276/410 (67.32%), Query Frame = 0

Query: 21  SSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRATSISRDCDNAVQNCDSGEEET 80
           SS+ ARRRRMEIRR KFV+     E E V     L+ R     R       +  +   ET
Sbjct: 34  SSQAARRRRMEIRRFKFVS----GEQEPVFVDGDLQRR----RRRESTVAASTSTVFYET 93

Query: 81  VGRFVV--GEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRRREMEDAVAVHPSLC-YTE 140
               VV    +S+  V++P           PK+G +SVCGRRREMEDAVAVHP    +  
Sbjct: 94  AKEVVVLCESLSSTVVALPDPE------AYPKYGVASVCGRRREMEDAVAVHPFFSRHQT 153

Query: 141 KRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKEDAAGAAETEAETASRWD 200
           + +S   H+ GVYDGHGCSHVAM+C+ERLHELV++E + D                + W+
Sbjct: 154 EYSSTGFHYCGVYDGHGCSHVAMKCRERLHELVREEFEAD----------------ADWE 213

Query: 201 RTMKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTAVVAIVTPDKIIVANCGDS 260
           ++M R F RMD EVVA  N +  A CRCELQ PDCDAVGSTAVV+++TP+KIIVANCGDS
Sbjct: 214 KSMARSFTRMDMEVVAL-NADGAAKCRCELQRPDCDAVGSTAVVSVLTPEKIIVANCGDS 273

Query: 261 RAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPRVLGVLAMSRAIGDNYLKP 320
           RAVLCRNGKAI LSSDHKPDRPDEL RI+ AGGRVIYWDGPRVLGVLAMSRAIGDNYLKP
Sbjct: 274 RAVLCRNGKAIALSSDHKPDRPDELDRIQAAGGRVIYWDGPRVLGVLAMSRAIGDNYLKP 333

Query: 321 YVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASMCLRGKAEERSPVSPSSEA 380
           YVIS PEVT+T+R   D+ LIL SDGLWDVV NETAC V  MCLRGK   +   SP  E 
Sbjct: 334 YVISRPEVTVTDRANGDDFLILASDGLWDVVSNETACSVVRMCLRGKVNGQVSSSPEREM 393

Query: 381 ETAAEGEERGNA----DKACNDASMLLTKLALARHSTDNVSVVVVDLKRD 424
                G          DKAC +AS+LLT+LALAR S+DNVSVVVVDL+RD
Sbjct: 394 TGVGAGNVVVGGGDLPDKACEEASLLLTRLALARQSSDNVSVVVVDLRRD 412

BLAST of Cucsat.G8361 vs. ExPASy Swiss-Prot
Match: Q9ZW21 (Probable protein phosphatase 2C 24 OS=Arabidopsis thaliana OX=3702 GN=At2g29380 PE=1 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 9.0e-112
Identity = 211/357 (59.10%), Postives = 258/357 (72.27%), Query Frame = 0

Query: 71  QNCDSGEEETVGRFVVGEVSTKEVSIPLS-----RESSCGVVVPKFGFSSVCGRRREMED 130
           +NC   ++E         ++T+  SIP S      + +  V  P++G SSVCGRRREMED
Sbjct: 41  KNCKRSKQEA--------LATRYSSIPRSSREDFSDQNVDVSSPRYGVSSVCGRRREMED 100

Query: 131 AVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKEDAAGA 190
           AVA+HPS   + K +    H+FGVYDGHGCSHVA RC+ERLH+LV++EL  D +++    
Sbjct: 101 AVAIHPSFS-SPKNSEFPQHYFGVYDGHGCSHVAARCRERLHKLVQEELSSDMEDE---- 160

Query: 191 AETEAETASRWDRTMKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTAVVAIVT 250
                     W  TM+R F RMD EVV+  +  V ANC+C+LQ+P CD+VGSTAVV+++T
Sbjct: 161 --------EEWKTTMERSFTRMDKEVVSWGDSVVTANCKCDLQTPACDSVGSTAVVSVIT 220

Query: 251 PDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPRVLGVLA 310
           PDKI+VANCGDSRAVLCRNGK +PLS+DHKPDRPDEL RIE AGGRVIYWD PRVLGVLA
Sbjct: 221 PDKIVVANCGDSRAVLCRNGKPVPLSTDHKPDRPDELDRIEGAGGRVIYWDCPRVLGVLA 280

Query: 311 MSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASMCLRGKA 370
           MSRAIGDNYLKPYV  EPEVTIT+R  +D+CLIL SDGLWDVV NETAC VA MCLRG  
Sbjct: 281 MSRAIGDNYLKPYVSCEPEVTITDR-RDDDCLILASDGLWDVVSNETACSVARMCLRGGG 340

Query: 371 EERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVDLKR 423
             +             + E+   +DKAC +AS+LLTKLALAR+S+DNVSVVV+DL+R
Sbjct: 341 RRQ-------------DNEDPAISDKACTEASVLLTKLALARNSSDNVSVVVIDLRR 362

BLAST of Cucsat.G8361 vs. ExPASy Swiss-Prot
Match: P49598 (Protein phosphatase 2C 37 OS=Arabidopsis thaliana OX=3702 GN=PP2CA PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.6e-111
Identity = 235/436 (53.90%), Postives = 285/436 (65.37%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRRRME-IRRCKFV--TRVDPSETETVSKRQKLED 60
           M  ICC V+ E+E ++  + +SR + RRR++ +   K V  + V P   E   KRQK E 
Sbjct: 1   MAGICCGVVGETEPAAPVDSTSRASLRRRLDLLPSIKIVADSAVAP-PLENCRKRQKRET 60

Query: 61  RATSI---SRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFS 120
              S    + D D+ V++    E +     V    S  E     S        VPK G +
Sbjct: 61  VVLSTLPGNLDLDSNVRS----ENKKARSAVTNSNSVTEAESFFSD-------VPKIGTT 120

Query: 121 SVCGRRREMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDEL 180
           SVCGRRR+MEDAV++HPS     +R S+  HF+GV+DGHGCSHVA +C+ERLH++VK E+
Sbjct: 121 SVCGRRRDMEDAVSIHPSFL---QRNSENHHFYGVFDGHGCSHVAEKCRERLHDIVKKEV 180

Query: 181 DKDEKEDAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVV--------ANCRCE 240
                         E   +  W  TM + F +MD EV  R    VV         +CRCE
Sbjct: 181 --------------EVMASDEWTETMVKSFQKMDKEVSQRECNLVVNGATRSMKNSCRCE 240

Query: 241 LQSPDCDAVGSTAVVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIE 300
           LQSP CDAVGSTAVV++VTP+KIIV+NCGDSRAVLCRNG AIPLS DHKPDRPDEL RI+
Sbjct: 241 LQSPQCDAVGSTAVVSVVTPEKIIVSNCGDSRAVLCRNGVAIPLSVDHKPDRPDELIRIQ 300

Query: 301 EAGGRVIYWDGPRVLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWD 360
           +AGGRVIYWDG RVLGVLAMSRAIGDNYLKPYVI +PEVT+T+RT EDECLIL SDGLWD
Sbjct: 301 QAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPDPEVTVTDRTDEDECLILASDGLWD 360

Query: 361 VVPNETACGVASMCLRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALA 420
           VVPNETACGVA MCLRG                A  G++   A  AC+DA++LLTKLALA
Sbjct: 361 VVPNETACGVARMCLRG----------------AGAGDDSDAAHNACSDAALLLTKLALA 391

Query: 421 RHSTDNVSVVVVDLKR 423
           R S+DNVSVVVVDL++
Sbjct: 421 RQSSDNVSVVVVDLRK 391

BLAST of Cucsat.G8361 vs. ExPASy Swiss-Prot
Match: Q9LNW3 (Protein phosphatase 2C 3 OS=Arabidopsis thaliana OX=3702 GN=AIP1 PE=1 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 5.9e-111
Identity = 236/458 (51.53%), Postives = 296/458 (64.63%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRR-RMEIRRCKFVTRVDPSETETVSKRQKLEDRA 60
           M +IC +     + +S+CE     + R+ R+ ++R     R+ PSE    +   + ED++
Sbjct: 1   MADICYE-----DETSACESRPLWSSRKWRIGVQR----FRMSPSEMNPTASTTEEEDKS 60

Query: 61  TSISR-----------DCDNAVQNCDSGEEETVG------RFVVGEVSTKEVS-IPLSRE 120
             I             +C ++  +  S EEE+V           G  S  +V+ IP  + 
Sbjct: 61  EGIYNKRNKQEEYDFMNCASSSPSQSSPEEESVSLEDSDVSISDGNSSVNDVAVIPSKKT 120

Query: 121 SSCGVVVPKFGFSSVCGRRREMEDAVAVHPSLCYTEKRAS-DMLHFFGVYDGHGCSHVAM 180
                + P++G +SVCGRRR+MEDAVA+HPS    +   S    H+FGVYDGHGCSHVA 
Sbjct: 121 VKETDLRPRYGVASVCGRRRDMEDAVALHPSFVRKQTEFSRTRWHYFGVYDGHGCSHVAA 180

Query: 181 RCKERLHELVKDELDKDEKEDAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVV 240
           RCKERLHELV++E   D+KE+              W + M+R F RMD EVV      + 
Sbjct: 181 RCKERLHELVQEEALSDKKEE--------------WKKMMERSFTRMDKEVVRWGETVMS 240

Query: 241 ANCRCELQSPDCDAVGSTAVVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPD 300
           ANCRCELQ+PDCDAVGSTAVV+++TP+KIIVANCGDSRAVLCRNGKA+PLS+DHKPDRPD
Sbjct: 241 ANCRCELQTPDCDAVGSTAVVSVITPEKIIVANCGDSRAVLCRNGKAVPLSTDHKPDRPD 300

Query: 301 ELSRIEEAGGRVIYWDGPRVLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILG 360
           EL RI+EAGGRVIYWDG RVLGVLAMSRAIGDNYLKPYV SEPEVT+T+RT EDE LIL 
Sbjct: 301 ELDRIQEAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVTSEPEVTVTDRTEEDEFLILA 360

Query: 361 SDGLWDVVPNETACGVASMCLR---GKAEERSPV-SPSSEAETAAEGEE----------- 420
           +DGLWDVV NE AC +  MCL    G+   R    +P   +E   + EE           
Sbjct: 361 TDGLWDVVTNEAACTMVRMCLNRKSGRGRRRGETQTPGRRSEEEGKEEEEKVVGSRKNGK 420

Query: 421 RGN-ADKACNDASMLLTKLALARHSTDNVSVVVVDLKR 423
           RG   DKAC +AS+LLTKLALA+HS+DNVSVVV+DL+R
Sbjct: 421 RGEITDKACTEASVLLTKLALAKHSSDNVSVVVIDLRR 435

BLAST of Cucsat.G8361 vs. ExPASy Swiss-Prot
Match: Q84JI0 (Probable protein phosphatase 2C 30 OS=Oryza sativa subsp. japonica OX=39947 GN=PP2C30 PE=1 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 9.7e-98
Identity = 222/451 (49.22%), Postives = 270/451 (59.87%), Query Frame = 0

Query: 1   MEEICCKVMSESEASS---SCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLED 60
           M EICC+V++ S +      C+  SR ARRRRMEIRR + V     +E ET  KR++L  
Sbjct: 1   MAEICCEVVAGSSSEGKGPECDTGSRAARRRRMEIRRLRVVAE-RGAEEETSGKRRRL-- 60

Query: 61  RATSISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVC 120
                           D G          GE ST E    + R         ++GF+SVC
Sbjct: 61  ----------------DGGG---------GEASTDEEDREVER--------ARYGFTSVC 120

Query: 121 GRRREMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKD 180
           GRRR+MED+V+  P             HFFGV+DGHGCSHVA  C +R+HE+V DE    
Sbjct: 121 GRRRDMEDSVSACPGFLPGH-------HFFGVFDGHGCSHVATSCGQRMHEIVVDEAGA- 180

Query: 181 EKEDAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEV--VANCRCELQSPDCDAV 240
               AAG+A  + E  +RW   M+R F RMD E VA +   V     CRCE+Q P CD V
Sbjct: 181 ----AAGSAGLDEE--ARWRGVMERSFARMDAEAVASSRGSVAPAPTCRCEMQLPKCDHV 240

Query: 241 GSTAVVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYW 300
           GSTAVVA++ P  ++VANCGDSRAVLCR G AIPLS DHKPDRPDEL RI  AGGRVI+W
Sbjct: 241 GSTAVVAVLGPRHVVVANCGDSRAVLCRGGAAIPLSCDHKPDRPDELERIHAAGGRVIFW 300

Query: 301 DGPRVLGVLAMSRAIGDNYLKPYVISEPEVTITNR-TAEDECLILGSDGLWDVVPNETAC 360
           DG RV G+LAMSRAIGD+YLKPYVI +PEV +  R   EDE LIL SDGLWDVV NE AC
Sbjct: 301 DGARVFGMLAMSRAIGDSYLKPYVICDPEVRVMERKDGEDEFLILASDGLWDVVSNEVAC 360

Query: 361 GVASMCLR--GKAEER--SPVSPSSEAETAAEGEERGN-------------------ADK 420
            V   CLR  G+ E    SP S  S  ++++ G+E  N                    DK
Sbjct: 361 NVVRACLRSSGRRERNRSSPTSNLSPRQSSSSGDEAPNDGAPSAAAGSESDEESAAEEDK 401

Query: 421 ACNDASMLLTKLALARHSTDNVSVVVVDLKR 423
           AC +A++LLTKLALAR ++DNVSVVVV+L+R
Sbjct: 421 ACAEAAVLLTKLALARQTSDNVSVVVVNLRR 401

BLAST of Cucsat.G8361 vs. NCBI nr
Match: XP_004145326.1 (probable protein phosphatase 2C 24 [Cucumis sativus] >KGN65754.1 hypothetical protein Csa_019966 [Cucumis sativus])

HSP 1 Score: 840 bits (2171), Expect = 3.08e-307
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60
           MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT
Sbjct: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60

Query: 61  SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRR 120
           SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRR
Sbjct: 61  SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRR 120

Query: 121 REMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKE 180
           REMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKE
Sbjct: 121 REMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKE 180

Query: 181 DAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTAV 240
           DAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTAV
Sbjct: 181 DAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTAV 240

Query: 241 VAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPRV 300
           VAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPRV
Sbjct: 241 VAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPRV 300

Query: 301 LGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASMC 360
           LGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASMC
Sbjct: 301 LGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASMC 360

Query: 361 LRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVDL 420
           LRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVDL
Sbjct: 361 LRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVDL 420

Query: 421 KRDAYP 426
           KRDAYP
Sbjct: 421 KRDAYP 426

BLAST of Cucsat.G8361 vs. NCBI nr
Match: XP_008457441.1 (PREDICTED: probable protein phosphatase 2C 24 [Cucumis melo] >TYJ97369.1 putative protein phosphatase 2C 24 [Cucumis melo var. makuwa])

HSP 1 Score: 791 bits (2044), Expect = 7.25e-288
Identity = 402/427 (94.15%), Postives = 413/427 (96.72%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60
           M EICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFV RVDP+ETETV+KRQKLEDRAT
Sbjct: 1   MAEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVARVDPTETETVAKRQKLEDRAT 60

Query: 61  SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRR 120
           SISRDCDNAVQNCDSGEEETVGRFV GEVSTKEVS PLSRES C VVVPKFG SSVCGRR
Sbjct: 61  SISRDCDNAVQNCDSGEEETVGRFVAGEVSTKEVSAPLSRESPCEVVVPKFGLSSVCGRR 120

Query: 121 REMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKE 180
           REMEDAVAVHPSLC+TEK+A+DMLHFFGVYDGHGCSHVAMRCKERLHELVKDELD++E E
Sbjct: 121 REMEDAVAVHPSLCFTEKKANDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDREEME 180

Query: 181 DAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVV-ANCRCELQSPDCDAVGSTA 240
           DAAGAAETEAETASRWDRTMKR+F RMDNEVVARNNEEVV ANCRCELQSPDCDAVGSTA
Sbjct: 181 DAAGAAETEAETASRWDRTMKRVFRRMDNEVVARNNEEVVVANCRCELQSPDCDAVGSTA 240

Query: 241 VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300
           VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR
Sbjct: 241 VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300

Query: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM 360
           VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM
Sbjct: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM 360

Query: 361 CLRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD 420
           CLRGKA+ERS +SPS EAETA EGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD
Sbjct: 361 CLRGKADERSALSPSREAETAVEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD 420

Query: 421 LKRDAYP 426
           LKRD +P
Sbjct: 421 LKRDTHP 427

BLAST of Cucsat.G8361 vs. NCBI nr
Match: KAA0067776.1 (putative protein phosphatase 2C 24 [Cucumis melo var. makuwa])

HSP 1 Score: 788 bits (2035), Expect = 1.71e-286
Identity = 400/427 (93.68%), Postives = 411/427 (96.25%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60
           M EICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFV RVDP+ETETV+KRQKLEDRAT
Sbjct: 1   MAEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVARVDPTETETVAKRQKLEDRAT 60

Query: 61  SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRR 120
           SISRDCDNAVQNCDSGEEETVGRFV GEVSTKEVS PLSRES C VVVPKFG SSVCGRR
Sbjct: 61  SISRDCDNAVQNCDSGEEETVGRFVAGEVSTKEVSAPLSRESPCEVVVPKFGLSSVCGRR 120

Query: 121 REMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKE 180
           REMEDAVAVHPSLC+TEK+A+DMLHFFGVYDGHGCSHVAMRCKERLHELVKDELD++E E
Sbjct: 121 REMEDAVAVHPSLCFTEKKANDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDREEME 180

Query: 181 DAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVV-ANCRCELQSPDCDAVGSTA 240
           DAAGAAETEAETASRWDRTMKR+F RMDNEVVARNNEEVV ANCRCELQSPDCDAVGSTA
Sbjct: 181 DAAGAAETEAETASRWDRTMKRVFRRMDNEVVARNNEEVVVANCRCELQSPDCDAVGSTA 240

Query: 241 VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300
            VAIVTPD IIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR
Sbjct: 241 AVAIVTPDNIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300

Query: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM 360
           VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM
Sbjct: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM 360

Query: 361 CLRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD 420
           CLRGKA+ERS +SPS EAETA EGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD
Sbjct: 361 CLRGKADERSALSPSREAETAVEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD 420

Query: 421 LKRDAYP 426
           LKRD +P
Sbjct: 421 LKRDTHP 427

BLAST of Cucsat.G8361 vs. NCBI nr
Match: XP_038895056.1 (probable protein phosphatase 2C 24 [Benincasa hispida])

HSP 1 Score: 770 bits (1989), Expect = 1.56e-279
Identity = 395/427 (92.51%), Postives = 404/427 (94.61%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60
           M EICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFV  V PSETETV+KRQKLEDRAT
Sbjct: 1   MAEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVAGVAPSETETVAKRQKLEDRAT 60

Query: 61  SISRDCDNAVQNCDSGEEETVGRFVVG-EVSTKEVSIPLSRESSCGVVVPKFGFSSVCGR 120
           SISRDCDNAVQNCDSGEEETVGRFV G EVSTKEVS PLSRES CG V+PKFGFSSVCGR
Sbjct: 61  SISRDCDNAVQNCDSGEEETVGRFVAGGEVSTKEVSTPLSRESPCGEVIPKFGFSSVCGR 120

Query: 121 RREMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEK 180
           RR+MEDAVAVHPSLC TEK+A+DMLHFFGVYDGHGCSHVAMRCKERLHELVKDELD++EK
Sbjct: 121 RRDMEDAVAVHPSLCLTEKKANDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDREEK 180

Query: 181 EDAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTA 240
           +DAA  AE EA   SRWDR MKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTA
Sbjct: 181 DDAAIVAEAEA---SRWDRAMKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTA 240

Query: 241 VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300
           VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR
Sbjct: 241 VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300

Query: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM 360
           VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLIL SDGLWDVVPNETACGVASM
Sbjct: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILASDGLWDVVPNETACGVASM 360

Query: 361 CLRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD 420
           CLRGKAEERSPV PS EAETAAEGEERG+ADKACNDASMLLTKLALARHSTDNVSVVVVD
Sbjct: 361 CLRGKAEERSPVWPSGEAETAAEGEERGSADKACNDASMLLTKLALARHSTDNVSVVVVD 420

Query: 421 LKRDAYP 426
           LK D  P
Sbjct: 421 LKGDTCP 424

BLAST of Cucsat.G8361 vs. NCBI nr
Match: KAG6607271.1 (Protein phosphatase 2C 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 684 bits (1766), Expect = 1.34e-245
Identity = 357/427 (83.61%), Postives = 379/427 (88.76%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60
           M EICCKV+ ESEASSSCEPSSRVARRRRMEIRRCKFV  V PSET+TV+KRQKLEDR+ 
Sbjct: 1   MAEICCKVIGESEASSSCEPSSRVARRRRMEIRRCKFVAGVAPSETDTVAKRQKLEDRSA 60

Query: 61  SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSC---GVVVPKFGFSSVC 120
           SISRDCDNAV+NCDSGEEETVGRFV GEV  KEVS PL RES     G  +PKFGFSSVC
Sbjct: 61  SISRDCDNAVENCDSGEEETVGRFVAGEVP-KEVSTPLHRESPIIPLGGEIPKFGFSSVC 120

Query: 121 GRRREMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKD 180
           GRRR+MEDAVAVHPSLC+T+K A+D LH FGVYDGHGCSHVAMRCK+RLHELVK+ELD++
Sbjct: 121 GRRRDMEDAVAVHPSLCFTDKEATDKLHLFGVYDGHGCSHVAMRCKDRLHELVKEELDRE 180

Query: 181 EKEDAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEE-VVANCRCELQSPDCDAVG 240
            KE+A+ AAE     ASRWD  MKRIF RMDNEVVARNNEE VVANCRCELQSPDCDAVG
Sbjct: 181 VKEEASAAAE-----ASRWDGAMKRIFRRMDNEVVARNNEEIVVANCRCELQSPDCDAVG 240

Query: 241 STAVVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWD 300
           STAVVA+VT +KIIVANCGDSRAVLCRNGKA+PLSSDHKPDRPDEL+RIEEAGGRVIYWD
Sbjct: 241 STAVVAVVTANKIIVANCGDSRAVLCRNGKAVPLSSDHKPDRPDELNRIEEAGGRVIYWD 300

Query: 301 GPRVLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGV 360
           GPRVLGVLAMSRAIGDNYLKPYVISEPEVTIT+RTAEDECLIL SDGLWDVVPNETACGV
Sbjct: 301 GPRVLGVLAMSRAIGDNYLKPYVISEPEVTITDRTAEDECLILASDGLWDVVPNETACGV 360

Query: 361 ASMCLRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVV 420
           ASMCLRGK E RSP SP    ETA E      ADKACNDAS+LLTKLALARHSTDNVSVV
Sbjct: 361 ASMCLRGKTEARSPGSPPVAVETAVERVGTERADKACNDASILLTKLALARHSTDNVSVV 420

Query: 421 VVDLKRD 423
           VVDLKRD
Sbjct: 421 VVDLKRD 421

BLAST of Cucsat.G8361 vs. ExPASy TrEMBL
Match: A0A0A0LXG6 (Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_1G524760 PE=3 SV=1)

HSP 1 Score: 840 bits (2171), Expect = 1.49e-307
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60
           MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT
Sbjct: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60

Query: 61  SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRR 120
           SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRR
Sbjct: 61  SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRR 120

Query: 121 REMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKE 180
           REMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKE
Sbjct: 121 REMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKE 180

Query: 181 DAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTAV 240
           DAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTAV
Sbjct: 181 DAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVVANCRCELQSPDCDAVGSTAV 240

Query: 241 VAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPRV 300
           VAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPRV
Sbjct: 241 VAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPRV 300

Query: 301 LGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASMC 360
           LGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASMC
Sbjct: 301 LGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASMC 360

Query: 361 LRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVDL 420
           LRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVDL
Sbjct: 361 LRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVDL 420

Query: 421 KRDAYP 426
           KRDAYP
Sbjct: 421 KRDAYP 426

BLAST of Cucsat.G8361 vs. ExPASy TrEMBL
Match: A0A5D3BEC3 (Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001800 PE=3 SV=1)

HSP 1 Score: 791 bits (2044), Expect = 3.51e-288
Identity = 402/427 (94.15%), Postives = 413/427 (96.72%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60
           M EICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFV RVDP+ETETV+KRQKLEDRAT
Sbjct: 1   MAEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVARVDPTETETVAKRQKLEDRAT 60

Query: 61  SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRR 120
           SISRDCDNAVQNCDSGEEETVGRFV GEVSTKEVS PLSRES C VVVPKFG SSVCGRR
Sbjct: 61  SISRDCDNAVQNCDSGEEETVGRFVAGEVSTKEVSAPLSRESPCEVVVPKFGLSSVCGRR 120

Query: 121 REMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKE 180
           REMEDAVAVHPSLC+TEK+A+DMLHFFGVYDGHGCSHVAMRCKERLHELVKDELD++E E
Sbjct: 121 REMEDAVAVHPSLCFTEKKANDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDREEME 180

Query: 181 DAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVV-ANCRCELQSPDCDAVGSTA 240
           DAAGAAETEAETASRWDRTMKR+F RMDNEVVARNNEEVV ANCRCELQSPDCDAVGSTA
Sbjct: 181 DAAGAAETEAETASRWDRTMKRVFRRMDNEVVARNNEEVVVANCRCELQSPDCDAVGSTA 240

Query: 241 VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300
           VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR
Sbjct: 241 VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300

Query: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM 360
           VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM
Sbjct: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM 360

Query: 361 CLRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD 420
           CLRGKA+ERS +SPS EAETA EGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD
Sbjct: 361 CLRGKADERSALSPSREAETAVEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD 420

Query: 421 LKRDAYP 426
           LKRD +P
Sbjct: 421 LKRDTHP 427

BLAST of Cucsat.G8361 vs. ExPASy TrEMBL
Match: A0A1S3C674 (Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103497127 PE=3 SV=1)

HSP 1 Score: 791 bits (2044), Expect = 3.51e-288
Identity = 402/427 (94.15%), Postives = 413/427 (96.72%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60
           M EICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFV RVDP+ETETV+KRQKLEDRAT
Sbjct: 1   MAEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVARVDPTETETVAKRQKLEDRAT 60

Query: 61  SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRR 120
           SISRDCDNAVQNCDSGEEETVGRFV GEVSTKEVS PLSRES C VVVPKFG SSVCGRR
Sbjct: 61  SISRDCDNAVQNCDSGEEETVGRFVAGEVSTKEVSAPLSRESPCEVVVPKFGLSSVCGRR 120

Query: 121 REMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKE 180
           REMEDAVAVHPSLC+TEK+A+DMLHFFGVYDGHGCSHVAMRCKERLHELVKDELD++E E
Sbjct: 121 REMEDAVAVHPSLCFTEKKANDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDREEME 180

Query: 181 DAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVV-ANCRCELQSPDCDAVGSTA 240
           DAAGAAETEAETASRWDRTMKR+F RMDNEVVARNNEEVV ANCRCELQSPDCDAVGSTA
Sbjct: 181 DAAGAAETEAETASRWDRTMKRVFRRMDNEVVARNNEEVVVANCRCELQSPDCDAVGSTA 240

Query: 241 VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300
           VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR
Sbjct: 241 VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300

Query: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM 360
           VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM
Sbjct: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM 360

Query: 361 CLRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD 420
           CLRGKA+ERS +SPS EAETA EGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD
Sbjct: 361 CLRGKADERSALSPSREAETAVEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD 420

Query: 421 LKRDAYP 426
           LKRD +P
Sbjct: 421 LKRDTHP 427

BLAST of Cucsat.G8361 vs. ExPASy TrEMBL
Match: A0A5A7VMK4 (Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold352G001040 PE=3 SV=1)

HSP 1 Score: 788 bits (2035), Expect = 8.26e-287
Identity = 400/427 (93.68%), Postives = 411/427 (96.25%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60
           M EICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFV RVDP+ETETV+KRQKLEDRAT
Sbjct: 1   MAEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVARVDPTETETVAKRQKLEDRAT 60

Query: 61  SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSCGVVVPKFGFSSVCGRR 120
           SISRDCDNAVQNCDSGEEETVGRFV GEVSTKEVS PLSRES C VVVPKFG SSVCGRR
Sbjct: 61  SISRDCDNAVQNCDSGEEETVGRFVAGEVSTKEVSAPLSRESPCEVVVPKFGLSSVCGRR 120

Query: 121 REMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKDEKE 180
           REMEDAVAVHPSLC+TEK+A+DMLHFFGVYDGHGCSHVAMRCKERLHELVKDELD++E E
Sbjct: 121 REMEDAVAVHPSLCFTEKKANDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDREEME 180

Query: 181 DAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEEVV-ANCRCELQSPDCDAVGSTA 240
           DAAGAAETEAETASRWDRTMKR+F RMDNEVVARNNEEVV ANCRCELQSPDCDAVGSTA
Sbjct: 181 DAAGAAETEAETASRWDRTMKRVFRRMDNEVVARNNEEVVVANCRCELQSPDCDAVGSTA 240

Query: 241 VVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300
            VAIVTPD IIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR
Sbjct: 241 AVAIVTPDNIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWDGPR 300

Query: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM 360
           VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM
Sbjct: 301 VLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGVASM 360

Query: 361 CLRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD 420
           CLRGKA+ERS +SPS EAETA EGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD
Sbjct: 361 CLRGKADERSALSPSREAETAVEGEERGNADKACNDASMLLTKLALARHSTDNVSVVVVD 420

Query: 421 LKRDAYP 426
           LKRD +P
Sbjct: 421 LKRDTHP 427

BLAST of Cucsat.G8361 vs. ExPASy TrEMBL
Match: A0A6J1G9R9 (Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111452249 PE=3 SV=1)

HSP 1 Score: 682 bits (1759), Expect = 7.52e-245
Identity = 354/427 (82.90%), Postives = 379/427 (88.76%), Query Frame = 0

Query: 1   MEEICCKVMSESEASSSCEPSSRVARRRRMEIRRCKFVTRVDPSETETVSKRQKLEDRAT 60
           M EICCKV+ ESEASSSCEPSSRVARRRRMEIRRCKFV  V PSET+TV+KRQKLEDR+ 
Sbjct: 1   MAEICCKVIGESEASSSCEPSSRVARRRRMEIRRCKFVAGVAPSETDTVAKRQKLEDRSA 60

Query: 61  SISRDCDNAVQNCDSGEEETVGRFVVGEVSTKEVSIPLSRESSC---GVVVPKFGFSSVC 120
           S+SRDCDNAV+NCDSGEEETVG FV GEV  KEVS PL RES     GV +PKFGFSSVC
Sbjct: 61  SLSRDCDNAVENCDSGEEETVGSFVAGEVP-KEVSTPLHRESPIIPLGVEIPKFGFSSVC 120

Query: 121 GRRREMEDAVAVHPSLCYTEKRASDMLHFFGVYDGHGCSHVAMRCKERLHELVKDELDKD 180
           GRRR+MEDAVAVHPSLC+T+K A+D +H FGVYDGHGCSHVAMRCK+RLHELVK+ELD++
Sbjct: 121 GRRRDMEDAVAVHPSLCFTDKEATDKVHLFGVYDGHGCSHVAMRCKDRLHELVKEELDRE 180

Query: 181 EKEDAAGAAETEAETASRWDRTMKRIFWRMDNEVVARNNEE-VVANCRCELQSPDCDAVG 240
            KE+++ AAE     ASRWD  MKRIF RMDNEVVARNNEE VVANCRCELQSPDCDAVG
Sbjct: 181 VKEESSAAAE-----ASRWDGAMKRIFRRMDNEVVARNNEEIVVANCRCELQSPDCDAVG 240

Query: 241 STAVVAIVTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELSRIEEAGGRVIYWD 300
           STAVVA+VT +KIIVANCGDSRAVLCRNGKA+PLSSDHKPDRPDEL+RIEEAGGRVIYWD
Sbjct: 241 STAVVAVVTANKIIVANCGDSRAVLCRNGKAVPLSSDHKPDRPDELNRIEEAGGRVIYWD 300

Query: 301 GPRVLGVLAMSRAIGDNYLKPYVISEPEVTITNRTAEDECLILGSDGLWDVVPNETACGV 360
           GPRVLGVLAMSRAIGDNYLKPYVISEPEVTIT+RTAEDECLIL SDGLWDVVPNETACGV
Sbjct: 301 GPRVLGVLAMSRAIGDNYLKPYVISEPEVTITDRTAEDECLILASDGLWDVVPNETACGV 360

Query: 361 ASMCLRGKAEERSPVSPSSEAETAAEGEERGNADKACNDASMLLTKLALARHSTDNVSVV 420
           ASMCLRGK E RSP SP    ETA E      ADKACNDAS+LLTKLALARHSTDNVSVV
Sbjct: 361 ASMCLRGKTEARSPGSPPVAVETAVERVGTERADKACNDASILLTKLALARHSTDNVSVV 420

Query: 421 VVDLKRD 423
           VVDLKRD
Sbjct: 421 VVDLKRD 421

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FIF59.7e-11457.80Probable protein phosphatase 2C 78 OS=Arabidopsis thaliana OX=3702 GN=SAG113 PE=... [more]
Q9ZW219.0e-11259.10Probable protein phosphatase 2C 24 OS=Arabidopsis thaliana OX=3702 GN=At2g29380 ... [more]
P495982.6e-11153.90Protein phosphatase 2C 37 OS=Arabidopsis thaliana OX=3702 GN=PP2CA PE=1 SV=1[more]
Q9LNW35.9e-11151.53Protein phosphatase 2C 3 OS=Arabidopsis thaliana OX=3702 GN=AIP1 PE=1 SV=1[more]
Q84JI09.7e-9849.22Probable protein phosphatase 2C 30 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Match NameE-valueIdentityDescription
XP_004145326.13.08e-307100.00probable protein phosphatase 2C 24 [Cucumis sativus] >KGN65754.1 hypothetical pr... [more]
XP_008457441.17.25e-28894.15PREDICTED: probable protein phosphatase 2C 24 [Cucumis melo] >TYJ97369.1 putativ... [more]
KAA0067776.11.71e-28693.68putative protein phosphatase 2C 24 [Cucumis melo var. makuwa][more]
XP_038895056.11.56e-27992.51probable protein phosphatase 2C 24 [Benincasa hispida][more]
KAG6607271.11.34e-24583.61Protein phosphatase 2C 3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A0A0LXG61.49e-307100.00Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_1G524760 ... [more]
A0A5D3BEC33.51e-28894.15Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3C6743.51e-28894.15Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103497127 PE=... [more]
A0A5A7VMK48.26e-28793.68Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1G9R97.52e-24582.90Protein-serine/threonine phosphatase OS=Cucurbita moschata OX=3662 GN=LOC1114522... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001932PPM-type phosphatase domainSMARTSM00332PP2C_4coord: 5..259
e-value: 1.4E-55
score: 200.6
IPR001932PPM-type phosphatase domainPFAMPF00481PP2Ccoord: 70..217
e-value: 1.1E-53
score: 182.6
IPR001932PPM-type phosphatase domainPROSITEPS51746PPM_2coord: 1..261
score: 45.801182
IPR001932PPM-type phosphatase domainCDDcd00143PP2Cccoord: 3..261
e-value: 7.64328E-73
score: 221.816
IPR036457PPM-type phosphatase domain superfamilyGENE3D3.60.40.10coord: 1..265
e-value: 1.7E-87
score: 295.8
IPR036457PPM-type phosphatase domain superfamilySUPERFAMILY81606PP2C-likecoord: 30..262
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 206..232
NoneNo IPR availablePANTHERPTHR47992ALPHABET, ISOFORM E-RELATEDcoord: 1..264
NoneNo IPR availablePANTHERPTHR47992:SF93PROTEIN PHOSPHATASE 2C 37coord: 1..264

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G8361.T1Cucsat.G8361.T1mRNA
Cucsat.G8361.T4Cucsat.G8361.T4mRNA
Cucsat.G8361.T2Cucsat.G8361.T2mRNA
Cucsat.G8361.T3Cucsat.G8361.T3mRNA
Cucsat.G8361.T5Cucsat.G8361.T5mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006470 protein dephosphorylation
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004722 protein serine/threonine phosphatase activity
molecular_function GO:0043169 cation binding
molecular_function GO:0016791 phosphatase activity