Cucsat.G8156 (gene) Cucumber (B10) v3

BLAST of Cucsat.G8156 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 2.0e-196
Identity = 339/526 (64.45%), Postives = 411/526 (78.14%), Query Frame = 0

Query: 25  ILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLETYIRNL 84
           IL+L F   ++ SS+SS+  E F QCL  +S P +PI+PAIF   N SYSSVL+  IRNL
Sbjct: 6   ILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNL 65

Query: 85  RFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVS-DVVPFFI 144
           RFNT++TPKPFLI+ A HESHV+AAI C + HNLQMKIRSGGHDY+G+SYV+    PFF+
Sbjct: 66  RFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFV 125

Query: 145 LDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHFGGGG 204
           LDMFNLR + VD  +KTAWVQTGA LGEVYY + E +K+  +PAG+CPTVGVGGH  GGG
Sbjct: 126 LDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGG 185

Query: 205 YGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMV 264
           YGNMMRKYGL+VD  IDA+++DVNGK+LDRK MGEDL+WAI GGGG S+GVV+AYKI +V
Sbjct: 186 YGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLV 245

Query: 265 PVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRAT 324
            VPE VTVFR+ RTLEQN  T+I+  WQQVA  + ++LFIR   DVVNGT   +KT+R T
Sbjct: 246 EVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTT 305

Query: 325 FIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEALLSRTPQ 384
           FIA+FLGD+  LL ++N  FP+LGL +SDC E SW+QSVLFWTN  +G+S E LL +  Q
Sbjct: 306 FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS-ETLLLQRNQ 365

Query: 385 VLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAG 444
            + +LKRKSDYV+ PI + GL  IWKKMIELE P + FNPYGG M  I S  TPFP+RAG
Sbjct: 366 PVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAG 425

Query: 445 NLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVN-HNG 504
           NLWKIQY  NW +E   +   +++LTRKLY+FMTPFVSKNPR +F NYRD+D+G+N HNG
Sbjct: 426 NLWKIQYGANWRDETLTD--RYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNG 485

Query: 505 K-NSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 548
           K +SY EG+ YG KYF GNF+RLVKIKT+VD  NFFRNEQSIP  P
Sbjct: 486 KISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of Cucsat.G8156 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 1.2e-164
Identity = 296/519 (57.03%), Postives = 374/519 (72.06%), Query Frame = 0

Query: 33  LVFFSSA----SSSAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIRNLRFN 92
           L FFSSA    S S  E+F QC   +   P   I   +F+  N S+SSVL  YIRN RFN
Sbjct: 20  LPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRNGRFN 79

Query: 93  TSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFILDMF 152
           TS+TPKP +I+T   + HV AA+ C++  N  +KIRSGGHDYEG+SY+SD  PFFILDM 
Sbjct: 80  TSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISD-KPFFILDMS 139

Query: 153 NLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHFGGGGYGNM 212
           NLR+++VD   ++AW+  GATLGEVYYR+ E +K +GFPAGVCPTVGVGGH  GGGYGNM
Sbjct: 140 NLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGYGNM 199

Query: 213 MRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRMVPVPE 272
           +RK+GLSVD +IDAKI+DVNG++LDRK+MGEDLFWAI GGGG+SFGVV+ YK+++VPVPE
Sbjct: 200 LRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVPVPE 259

Query: 273 TVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRATFIAL 332
           TVTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RAT +AL
Sbjct: 260 TVTVFRVEKYMD-SGAVDMVHKWQSVGPKTDRNLFLRMLIQPV--TRKKVKTVRATVVAL 319

Query: 333 FLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSRTPQVL 392
           FLG +E ++ ++   FP+L LKK +C EM+W QS L+W N    T ++    L R     
Sbjct: 320 FLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRA 379

Query: 393 THLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPHRAGNL 452
              KRKSDYV + IP++G+  ++KKM EL K  L FNPYGG+MAE+  NATPFPHR+  L
Sbjct: 380 NFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KL 439

Query: 453 WKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNHNGKNS 512
           +KIQY+  W E   +  K F++    LY FMT FVSKNPR A+LNYRD+DIGVN +G NS
Sbjct: 440 FKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNS 499

Query: 513 YYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 545
           Y EG VYG KYF  NFDRLVK+KT  DP NFFRNEQSIP
Sbjct: 500 YEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of Cucsat.G8156 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 572.4 bits (1474), Expect = 5.5e-162
Identity = 290/524 (55.34%), Postives = 376/524 (71.76%), Query Frame = 0

Query: 30  FSFLVFFS------SASSSAPENFFQCLL-RHSPPSYPIAPAIFTPNNASYSSVLETYIR 89
           F FL+F S      S S+S   +F +C   +   P   I   +F+  N ++SSVL  YIR
Sbjct: 16  FFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIR 75

Query: 90  NLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFF 149
           N RFNTS+T KP +I+T   ESHV AA+ C++  N  +KIRSGGHDY+G+SY+SD  PFF
Sbjct: 76  NARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFF 135

Query: 150 ILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHFGGG 209
           ILDM N+R+++VD  + +AW+  GATLGEVYYR+ E ++ +GFPAGVCPTVGVGGH  GG
Sbjct: 136 ILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGG 195

Query: 210 GYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRM 269
           GYGNM+RK+GLSVD + DAKI+DVNG++LDRKAMGEDLFWAI GGGG S+GVV+ YK+++
Sbjct: 196 GYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKL 255

Query: 270 VPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKTLRA 329
           VPVP  VTVFRV++ ++ +   ++V +WQ V    D +LF+R+    V  T K  KT+RA
Sbjct: 256 VPVPSVVTVFRVEQYMD-SGAVDMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRA 315

Query: 330 TFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVE--ALLSR 389
           + +ALFLG ++ ++ +++  FP+LGLKK +C EM+W QS L+W N    T V+    L R
Sbjct: 316 SVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDR 375

Query: 390 TPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPFPH 449
                +  KRKSDYV T IPK+G+  ++KKMIEL K  L FNPYGG+MAE+  NA PFPH
Sbjct: 376 NLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPH 435

Query: 450 RAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVNH 509
           R   L+KIQY+ NW E   +  K +++  + LY FMT FVSKNPR+++ NYRD+DIGVN 
Sbjct: 436 R-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVND 495

Query: 510 NGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 545
           +G NSY EG VYG KYF  NFDRLVKIKT VDP NFFRNEQSIP
Sbjct: 496 HGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of Cucsat.G8156 vs. ExPASy Swiss-Prot
Match: Q9SA99 (Berberine bridge enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11770 PE=2 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 4.7e-161
Identity = 287/530 (54.15%), Postives = 371/530 (70.00%), Query Frame = 0

Query: 25  ILLLSFSFLVFFSSASSSAPE----NFFQCLLRHS-PPSYPIAPAIFTPNNASYSSVLET 84
           ++L   S  +  S A    PE    NF QC    +  P   +A  +     A+++ VL  
Sbjct: 6   LILFLISSFISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRA 65

Query: 85  YIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDVV 144
           YIRN RFNT+ TPKP +++ A  ESHV+AA++C +  N+Q+K RSGGHDYEGVSY+S  V
Sbjct: 66  YIRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISH-V 125

Query: 145 PFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGHF 204
           PFF+LDM NLR ITVD  T++AWV  GATLGEVYYR+ E  KS+GFPAGVCPTVG GGH 
Sbjct: 126 PFFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGAGGHI 185

Query: 205 GGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAYK 264
            GGGYGNM+RKYGLSVD + DAKI+DVNG++LDRK MGED+FWAI GGGG+SFGV++A+K
Sbjct: 186 SGGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFK 245

Query: 265 IRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKKT 324
           I++VPVP TVTVFRV++ L +N  TE+V +WQ VA   D  LF+R+    V  T    +T
Sbjct: 246 IKLVPVPPTVTVFRVEKNLVEN-ATEMVHKWQFVAPKTDPGLFMRLLLQPV--TRNKMQT 305

Query: 325 LRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSV--EAL 384
           +RA+ +ALFLGD   ++ ++   FP+LGLKK +C EM+W+QSV++W N    T +  E L
Sbjct: 306 VRASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEIL 365

Query: 385 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 444
           L R P + T  KRKSD+V+  I K+GL+ ++KKMIE+ K  L FNPYGG M+ + +  TP
Sbjct: 366 LDRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTP 425

Query: 445 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 504
           FPHR   L+KIQ++ NW + G +    F+   +  Y +M PFV+KNPR  ++NYRDLDIG
Sbjct: 426 FPHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIG 485

Query: 505 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 548
           VN  G NSY    V+G  YF  NFDRLVK+KT VDP NFFR+EQSIP  P
Sbjct: 486 VNTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530

BLAST of Cucsat.G8156 vs. ExPASy Swiss-Prot
Match: F4HV09 (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 1.3e-158
Identity = 281/534 (52.62%), Postives = 383/534 (71.72%), Query Frame = 0

Query: 14  TNTRMEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASY 73
           + T+  I    +LL+  SF     +A     ++F QC+     PS PI    +T  N ++
Sbjct: 4   STTQTLIFTVFLLLIPTSF-----AAPPKLKDSFTQCVTVFK-PSVPIQNFTYTQQNPNF 63

Query: 74  SSVLETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVS 133
            ++L  Y+RNLR+   TT KP  I+ A H +H++A I CA+   LQ++IRSGGHDY+G+S
Sbjct: 64  LTILNNYVRNLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMS 123

Query: 134 YVSDVVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVA-ENNKSYGFPAGVCPT 193
           Y+S  V F +LDMFNLR I +D K  TAWVQ+GATLGE+YY VA ++N   GFPAG+CP 
Sbjct: 124 YLS-TVDFVVLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPG 183

Query: 194 VGVGGHFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSF 253
           +G GGHF GGGYGNMMRKYGLS+D IIDAKI+D N ++LDR +MGEDLFWA+ GGG +SF
Sbjct: 184 LGAGGHFSGGGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASF 243

Query: 254 GVVVAYKIRMVPVPETVTVFRVQRTLEQNDL-TEIVDEWQQVAHVIDNDLFIRVTFDVVN 313
            VV+A+KI++VPVPE VTVF V+    +  + T++  +WQ++A  IDNDLFIR+T    N
Sbjct: 244 CVVLAWKIKLVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSSN 303

Query: 314 GTNKGKKTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLG 373
                 KT++A+F+ ++LG+SE+LL ++N  FP+LGL K++CIEM W++SVLFW + P G
Sbjct: 304 ------KTVKASFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPG 363

Query: 374 TS-VEALLSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAE 433
           T+    +L+R PQ   +LKRKSDYV+ PI K GL  I+K + E E   + +NPYGGRM+E
Sbjct: 364 TAPTSVMLNRIPQKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSE 423

Query: 434 IPSNATPFPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLN 493
           IP+  T FPHRAGN++KIQY++NW   G + A   +  T ++++ M+P+VSKNPR AFLN
Sbjct: 424 IPATETAFPHRAGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLN 483

Query: 494 YRDLDIGVNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIP 545
           YRD+DIG N N  ++Y EG+VYG+KYF+ NF+RLV++KT+VDP N FR EQSIP
Sbjct: 484 YRDIDIGKNLN--STYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

BLAST of Cucsat.G8156 vs. NCBI nr
Match: XP_004135723.1 (berberine bridge enzyme-like 8 [Cucumis sativus] >KAE8653304.1 hypothetical protein Csa_023313 [Cucumis sativus])

HSP 1 Score: 1082 bits (2798), Expect = 0.0
Identity = 533/533 (100.00%), Postives = 533/533 (100.00%), Query Frame = 0

Query: 18  MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 77
           MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 78  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 137
           ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 138 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 197
           VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 180

Query: 198 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 257
           HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 258 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 317
           YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK
Sbjct: 241 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 300

Query: 318 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 377
           KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 378 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 437
           LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420

Query: 438 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 497
           FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 498 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHVW 550
           VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHVW
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHVW 533

BLAST of Cucsat.G8156 vs. NCBI nr
Match: XP_008445642.1 (PREDICTED: reticuline oxidase-like protein [Cucumis melo] >KAA0047124.1 reticuline oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1036 bits (2679), Expect = 0.0
Identity = 510/532 (95.86%), Postives = 521/532 (97.93%), Query Frame = 0

Query: 18  MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 77
           M+ILR+SIL LSFSFLVFFSSASSSAPENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 78  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 137
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 138 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 197
           VVPFF+LDMFNLREITVDAKT+TAWVQTGATLGEVYYRVAE NKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 198 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 257
           HFGGGGYGNMMRK+GLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 258 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 317
           YKIRMV VPE VTVFRVQRTLEQN+LTEIVDEWQQVAHVIDNDLFIRVTFDVVNGT+K K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 318 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 377
           KTLRATFIALFLGDS RLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 378 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 437
           LSRTPQVLTHLKRKSDYVK PIPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 438 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 497
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 498 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 549
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Cucsat.G8156 vs. NCBI nr
Match: XP_038895093.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 1021 bits (2641), Expect = 0.0
Identity = 501/532 (94.17%), Postives = 518/532 (97.37%), Query Frame = 0

Query: 18  MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 77
           MEILR+SILLLSFS+LVFFSSA+S APENFFQCLLRHSPPSYPIAPAIFTPNN S+SSVL
Sbjct: 1   MEILRSSILLLSFSYLVFFSSATS-APENFFQCLLRHSPPSYPIAPAIFTPNNVSFSSVL 60

Query: 78  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 137
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 120

Query: 138 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 197
           V+PFFILDMFNLR+ITVD KTKTAWVQTGATLGEVYYR+AE NK YGFPAGVCPTVGVGG
Sbjct: 121 VLPFFILDMFNLRDITVDVKTKTAWVQTGATLGEVYYRIAEKNKFYGFPAGVCPTVGVGG 180

Query: 198 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 257
           HFGGGGYGNMMRKYGLSVDQIIDAKI+DVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 258 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 317
           YKIRMV VPETVTVFRVQRTLEQNDLTEIVDEWQ+VAHVIDNDLFIRVTFDV+NGT KGK
Sbjct: 241 YKIRMVRVPETVTVFRVQRTLEQNDLTEIVDEWQRVAHVIDNDLFIRVTFDVINGTEKGK 300

Query: 318 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 377
           KTLRATF+A+FLGDSERLL VI  SFPKLGLKKSDC+EMSWLQSVLFWTNFPLGTSV+AL
Sbjct: 301 KTLRATFLAMFLGDSERLLSVIKKSFPKLGLKKSDCLEMSWLQSVLFWTNFPLGTSVDAL 360

Query: 378 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 437
           LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420

Query: 438 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 497
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGTQEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 498 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 549
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 531

BLAST of Cucsat.G8156 vs. NCBI nr
Match: XP_022994779.1 (berberine bridge enzyme-like 8 [Cucurbita maxima])

HSP 1 Score: 962 bits (2488), Expect = 0.0
Identity = 473/533 (88.74%), Postives = 501/533 (94.00%), Query Frame = 0

Query: 17  RMEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSV 76
           +MEI R SI LLSFS L+FF+SA++ APENF QCLLRHSPPSYPIAPAIFT NNASYSSV
Sbjct: 2   KMEISRFSIFLLSFSALLFFASATT-APENFLQCLLRHSPPSYPIAPAIFTSNNASYSSV 61

Query: 77  LETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVS 136
           LETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVS
Sbjct: 62  LETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVS 121

Query: 137 DVVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVG 196
           DVVPFF+LDMFNLR+I+VD KT+TAWVQTGATLGEVYYR+AE  K +GFPAGVCPTVGVG
Sbjct: 122 DVVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVG 181

Query: 197 GHFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVV 256
           GHFGGGGYGNMMRKYGLSVDQIIDAKI+DV G LLDRKAMGEDLFWAIIGGGGSSFGVVV
Sbjct: 182 GHFGGGGYGNMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVV 241

Query: 257 AYKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKG 316
           AYKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  G
Sbjct: 242 AYKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSG 301

Query: 317 KKTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEA 376
           KKTLRATFIALFLGDSERLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ 
Sbjct: 302 KKTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDE 361

Query: 377 LLSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNAT 436
           LLSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNAT
Sbjct: 362 LLSRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNAT 421

Query: 437 PFPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDI 496
           PFPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDI
Sbjct: 422 PFPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDI 481

Query: 497 GVNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 549
           GVNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP+V
Sbjct: 482 GVNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYV 533

BLAST of Cucsat.G8156 vs. NCBI nr
Match: XP_023542409.1 (berberine bridge enzyme-like 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 962 bits (2487), Expect = 0.0
Identity = 473/531 (89.08%), Postives = 499/531 (93.97%), Query Frame = 0

Query: 17  RMEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSV 76
           +MEI R S  LLSFS L+FF+SA++ APENF QCLLRHSPPSYPIAPAIFT NNASYSSV
Sbjct: 2   KMEISRFSSFLLSFSALLFFASATT-APENFLQCLLRHSPPSYPIAPAIFTSNNASYSSV 61

Query: 77  LETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVS 136
           LETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVS
Sbjct: 62  LETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVS 121

Query: 137 DVVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVG 196
           DVVPFF+LDMFNLR+I+VD KTKTAWVQTGATLGEVYYR+AE  K +GFPAGVCPTVGVG
Sbjct: 122 DVVPFFVLDMFNLRDISVDVKTKTAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVG 181

Query: 197 GHFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVV 256
           GHFGGGGYGNMMRKYGLSVDQIIDAKI+DVNG LLDRKAMGEDLFWAIIGGGGSSFGVVV
Sbjct: 182 GHFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVV 241

Query: 257 AYKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKG 316
           AYKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  G
Sbjct: 242 AYKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSKSG 301

Query: 317 KKTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEA 376
           KKTLRATFIALFLGDSERLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ 
Sbjct: 302 KKTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDE 361

Query: 377 LLSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNAT 436
           LLSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNAT
Sbjct: 362 LLSRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNAT 421

Query: 437 PFPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDI 496
           PFPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDI
Sbjct: 422 PFPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDI 481

Query: 497 GVNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 547
           GVNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP
Sbjct: 482 GVNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531

BLAST of Cucsat.G8156 vs. ExPASy TrEMBL
Match: A0A5A7TVZ2 (Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold466G001070 PE=3 SV=1)

HSP 1 Score: 1036 bits (2679), Expect = 0.0
Identity = 510/532 (95.86%), Postives = 521/532 (97.93%), Query Frame = 0

Query: 18  MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 77
           M+ILR+SIL LSFSFLVFFSSASSSAPENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 78  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 137
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 138 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 197
           VVPFF+LDMFNLREITVDAKT+TAWVQTGATLGEVYYRVAE NKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 198 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 257
           HFGGGGYGNMMRK+GLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 258 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 317
           YKIRMV VPE VTVFRVQRTLEQN+LTEIVDEWQQVAHVIDNDLFIRVTFDVVNGT+K K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 318 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 377
           KTLRATFIALFLGDS RLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 378 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 437
           LSRTPQVLTHLKRKSDYVK PIPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 438 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 497
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 498 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 549
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Cucsat.G8156 vs. ExPASy TrEMBL
Match: A0A1S3BE21 (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103488597 PE=3 SV=1)

HSP 1 Score: 1036 bits (2679), Expect = 0.0
Identity = 510/532 (95.86%), Postives = 521/532 (97.93%), Query Frame = 0

Query: 18  MEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVL 77
           M+ILR+SIL LSFSFLVFFSSASSSAPENF QCLLRHSPPSYPIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 78  ETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSD 137
           ETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 138 VVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGG 197
           VVPFF+LDMFNLREITVDAKT+TAWVQTGATLGEVYYRVAE NKSYGFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 198 HFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 257
           HFGGGGYGNMMRK+GLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 258 YKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGK 317
           YKIRMV VPE VTVFRVQRTLEQN+LTEIVDEWQQVAHVIDNDLFIRVTFDVVNGT+K K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 318 KTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 377
           KTLRATFIALFLGDS RLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 378 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 437
           LSRTPQVLTHLKRKSDYVK PIPKEGL+KIWKKMIELEKPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 438 FPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 497
           FPHRAGNLWKIQYATNW EEG +EAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 498 VNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 549
           VNHNGKNSYYEGRVYGIKYFEGNF+RLVKIKTKVDPHNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Cucsat.G8156 vs. ExPASy TrEMBL
Match: A0A6J1K3U3 (berberine bridge enzyme-like 8 OS=Cucurbita maxima OX=3661 GN=LOC111490407 PE=3 SV=1)

HSP 1 Score: 962 bits (2488), Expect = 0.0
Identity = 473/533 (88.74%), Postives = 501/533 (94.00%), Query Frame = 0

Query: 17  RMEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSV 76
           +MEI R SI LLSFS L+FF+SA++ APENF QCLLRHSPPSYPIAPAIFT NNASYSSV
Sbjct: 2   KMEISRFSIFLLSFSALLFFASATT-APENFLQCLLRHSPPSYPIAPAIFTSNNASYSSV 61

Query: 77  LETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVS 136
           LETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVS
Sbjct: 62  LETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVS 121

Query: 137 DVVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVG 196
           DVVPFF+LDMFNLR+I+VD KT+TAWVQTGATLGEVYYR+AE  K +GFPAGVCPTVGVG
Sbjct: 122 DVVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVG 181

Query: 197 GHFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVV 256
           GHFGGGGYGNMMRKYGLSVDQIIDAKI+DV G LLDRKAMGEDLFWAIIGGGGSSFGVVV
Sbjct: 182 GHFGGGGYGNMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVV 241

Query: 257 AYKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKG 316
           AYKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  G
Sbjct: 242 AYKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSG 301

Query: 317 KKTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEA 376
           KKTLRATFIALFLGDSERLL V+N SFPKLGLKKSDC+EMSWLQSVLFWTNFP GTSV+ 
Sbjct: 302 KKTLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDE 361

Query: 377 LLSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNAT 436
           LLSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNAT
Sbjct: 362 LLSRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNAT 421

Query: 437 PFPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDI 496
           PFPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDI
Sbjct: 422 PFPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDI 481

Query: 497 GVNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 549
           GVNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP+V
Sbjct: 482 GVNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYV 533

BLAST of Cucsat.G8156 vs. ExPASy TrEMBL
Match: A0A6J1GSQ1 (berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111456785 PE=3 SV=1)

HSP 1 Score: 957 bits (2475), Expect = 0.0
Identity = 470/533 (88.18%), Postives = 500/533 (93.81%), Query Frame = 0

Query: 17  RMEILRTSILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSV 76
           +M+I R S  LLSFS L+FF+SA++ APENF QCLLRHSPPSYPIAPAIFT NNASYSSV
Sbjct: 2   KMDISRFSSFLLSFSALLFFASATT-APENFLQCLLRHSPPSYPIAPAIFTSNNASYSSV 61

Query: 77  LETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVS 136
           LETYIRNLRFNTSTTPKPFLILTALHESHVK AILCARIH+LQMKIRSGGHDYEGVSYVS
Sbjct: 62  LETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVS 121

Query: 137 DVVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVG 196
           DVVPFF+LDMFNLR+I+VD KT+TAWVQTGATLGEVYYR+AE  K +GFPAGVCPTVGVG
Sbjct: 122 DVVPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVG 181

Query: 197 GHFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVV 256
           GHFGGGGYGNMMRKYGLSVDQIIDAKI+DVNG LLDRKAMGEDLFWAIIGGGGSSFGVVV
Sbjct: 182 GHFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVV 241

Query: 257 AYKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKG 316
           AYKIR V VPETVTVFRV RTLE ND+TEIVD+WQ+VAHVID DLFIRVTFDVVNG+  G
Sbjct: 242 AYKIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSG 301

Query: 317 KKTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEA 376
           KKTLRATFIALFLGDSERLL V+N SFPKLGLKK+DC+EMSWLQSVLFWTNFP GTSV+ 
Sbjct: 302 KKTLRATFIALFLGDSERLLSVMNKSFPKLGLKKADCLEMSWLQSVLFWTNFPQGTSVDE 361

Query: 377 LLSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNAT 436
           LLSRTPQVLTHLKRKSDYVKT IPKE LN+IWKKMIELEKP +TFNPYGGRMAEIPSNAT
Sbjct: 362 LLSRTPQVLTHLKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNAT 421

Query: 437 PFPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDI 496
           PFPHRAGNLWKIQYATNW E+G ++A HFIDLTRKLYKFMTPFV+KNPRTAFLNYRDLDI
Sbjct: 422 PFPHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDI 481

Query: 497 GVNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 549
           GVNHNGK SYYEGRV+GIKYFEGNFDRLVKIKTKVDP NFFRNEQSIPRFP+V
Sbjct: 482 GVNHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYV 533

BLAST of Cucsat.G8156 vs. ExPASy TrEMBL
Match: A0A6J1DGF3 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020214 PE=3 SV=1)

HSP 1 Score: 915 bits (2366), Expect = 0.0
Identity = 448/531 (84.37%), Postives = 493/531 (92.84%), Query Frame = 0

Query: 20  ILRTSILL-LSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSVLE 79
           ILR   LL L FS L F S+ ++++PENF +CLLR SPPS+PI+PAIFTP+NAS+SSVL+
Sbjct: 5   ILRPFFLLSLLFSLLFFASATTTASPENFLKCLLRRSPPSHPISPAIFTPDNASFSSVLQ 64

Query: 80  TYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVSDV 139
           TYIRNLRFNTS+TPKPFLILTALHESHVK AILCARIHNLQMKIRSGGHDYEGVSYVSDV
Sbjct: 65  TYIRNLRFNTSSTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDV 124

Query: 140 VPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVGGH 199
           VPFF+LDMFNLR ITVD +T+TAWV+TGATLGEVYYR+AE +K +GFPAGVCPTVGVGGH
Sbjct: 125 VPFFVLDMFNLRAITVDVETETAWVETGATLGEVYYRIAEKSKLHGFPAGVCPTVGVGGH 184

Query: 200 FGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 259
           FGGGGYGNMMRKYGLSVDQIIDAK++DVNGKLLDRK+MGEDLFWAI GGGGSSFG VVAY
Sbjct: 185 FGGGGYGNMMRKYGLSVDQIIDAKMVDVNGKLLDRKSMGEDLFWAITGGGGSSFGXVVAY 244

Query: 260 KIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKGKK 319
           KI+ V VPETVTVF+V+R L +N +TEIVDEWQ+VAH IDNDLFIRVTFDVVNGT  GKK
Sbjct: 245 KIQTVRVPETVTVFKVERRLAEN-VTEIVDEWQRVAHEIDNDLFIRVTFDVVNGTENGKK 304

Query: 320 TLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEALL 379
           TLRATFIALFLGDSERLL +++ SFPKLGLK+SDC++MSWL+SVLFWTNFPLGTSV+ALL
Sbjct: 305 TLRATFIALFLGDSERLLSIMDKSFPKLGLKQSDCLQMSWLESVLFWTNFPLGTSVDALL 364

Query: 380 SRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 439
           SR PQVLTHLKRKSDYVKT IPKEGLN+IWKKMIELEKPMLTFNPYGGRMAEI S+ TPF
Sbjct: 365 SRKPQVLTHLKRKSDYVKTLIPKEGLNQIWKKMIELEKPMLTFNPYGGRMAEIASSKTPF 424

Query: 440 PHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGV 499
           PHRAGNLWKIQYATNW EEG +EA HFI+LTRKLY FMTPFVSKNPRTAFLNYRDLDIGV
Sbjct: 425 PHRAGNLWKIQYATNWDEEGVEEADHFINLTRKLYNFMTPFVSKNPRTAFLNYRDLDIGV 484

Query: 500 NHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 549
           NHNGKNSY EGRV+G+KYFEGNFDRLVKIKT+VDP+NFFRNEQSIPR P+V
Sbjct: 485 NHNGKNSYNEGRVFGVKYFEGNFDRLVKIKTRVDPNNFFRNEQSIPRLPYV 534