Cucsat.G7182 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G7182
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Pectinesterase
Location	ctg1528: 3118674 .. 3123188 (-)
RNA-Seq Expression	Cucsat.G7182
Synteny	Cucsat.G7182

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTCTTTTTTTCTTTTTTTGCCAATTTACTACAAACACCACCACTCCCCAAAAAAGAACTACCCATCCTCTCAACACCAAAATTACCAAACACGTATTGACTTTAAATAATGATTTTAAAAAATACGAATTTCCCATTTTACCCTTTTGTTCATAAAGAACTACAATAACGCCCTCTCTCTTCTCTTATTTTATCCCAACCCCAAAAAACCGAAACACAAGGAGATTTTCGGGAGAGAATGTTCCGGCCGACCGTTGCTTCATCTTTTCCGGCGACGGTGGCGTTCGCAATGGGGGTGATGCTCCAACTCATTATACTAATAATTATTCCGTCCACCGTCGTCGTTGAAGCTCACACAAAAACAATTCCGGCAGATTCATCGAAGCTCGACGAATGGATCGGACACAACATGAAAGAATACAACGATCGTAAGACGAACGAGACAGGAATCAAGGCGCTGGACCGGCGGCTGGCGGAGGCGGAGGATTGTGTTCAATTAATCACCGTTAGAAAAGACGGACGAGGAAATTTCTCGACGATCACTGAAGCGATCGATAGCATTCCGTCAGGAAATCGGCGGCGAGTGGTGGTGTGGATCGGAGGTGGGGTTTATAGAGAGAAAATTACGATCGATGCTTCGAAGCCGTTTGTGACTTTGTATGGACAGAAAGGAAAACGGCCAATGATAACGTTCGACGGTACAGCGTCGGAGTTTGGTACCGTTAAGAGCGCGACGGTGGCGGTGGAATCGGATTATTTCGTTGCCGTCAATCTTACGTTTGTGGTGAGTACTTCTTCTTCTTCTTCTCAATTTTGTGGGGTACGTTTTGTAAATAGTAATAATTATGGGGCTATAAGTTTTTGGTAACGTAGGATGTGCACGAGTTTTCCATTTTTCCTTTTTCAAATTTAAATTTTACTAAACAAACAAATATGACTCATGCAATTCACCCGTCATCAAAATTATTCCATATCATGCCATTCCTCATGCGTACAAAACATGATTTTCTTCACCTTCAAATCTTGACTATATATATATGTGAAAATTATTGTTTAATAAGACTCTTACGCAATAAATGGTTTAAGGTGGACAAAAGTTTTACGTGAAGATTATTTTTAAATATAACAAAATGAATTAAAATATTTTCAAATTTAATAAAATACAACGATATTTTATTATAATGATAAATAATTTTTGTATATAATAATTTCTACGATAAATAAATTCATGTGTTTGGTATATAATTAGTTTGGGATAGGGTATGTTTATTCAAATATGGGACGAAATTTATAATTTGACATTTTCGATAGAGATAAAGAGGATTTAAACTACATATCTTTTTATTTGCTAATTATGCATATTTGATACATACATATGTTAGAGCATACTCATTTTGTCTTTAATTATCATATCTATTTTGTAGTCCATATGTAAAAATGGTGCTGCTTTCCATTTAAAAGAATGTGCAATGAGTTAATTGATCTGCACGACTTTAATTTGTTTAGTATTTACCAAAGTTCGTTTCTTAGAATTGGAAAAGTGGATGTGTATAGAAAAGGTAATTGAAAGAAGACTTTGTTGAAAAATCCCTCATGATTATTAGAAAAATATAAGAGCTAGTTGCATTTTAAAATTAATTACGTTCCTTATAAATGCCTAGTTTAGATGTTGCTTCTTTGGAGTCAATTCACTATTTCTAGGTATGTTGAATCTTAGTTTTTAGACCGAAAATACATTTGGACTTCTTGAACACATTGATATACCATATTAGATAAACGCTAGATTTTTATCTTAAAAATGATCAACTAACAATAGAAGAAGTACCTTGTGTATTTATTAAAGAGATTATTATGAAGTTGGCTTGATCTTCAACAATTATAACAAGTTTGAAAAGGACACATGAAAGAACCGATATCACATTCGGATAAGTATAGTTAAAAAAAGATGTCTAAAAGAACTAAAATACATGTACAAAAAAGTGAGCTTTTCTTTCGAGAGACCAAATCATAAGCATTCCAAACTTAACCATGTTTAATTTTGGAGCAACTATGTGTTTCAAGATAAGTAAGGATTACGTACTCAAGTCGCAAGGTAAATACGAGAGTAATTAGGTACTGAATCTTGGCACGCACGAGTCATTACACTAATGATCTTTTGTACTTTTTGAAAAAGAAAAAGGTGAGAATTGGTAAGAAACCATATATATAACATTATATTAGTAAAAAGTGATAATTTATATTGATGAAGAAATGAAGTTTGGTTGTAAATTTGTTGTGCAGAATTCAGCTCCAATGCCAGAACTAGGAGGAACGGGAGGGCAAGCAGTAGCAATGAGGATATCTGGTGATAAGGCAGCATTCCATGGGTGTCATTTCATAGGCTTTCAAGACACTTTATGTGATGACAGAGGCAGACATTTTTTCAAGGACTGTTACGTCCAAGGCACTGTGGACTTCATCTTTGGAAATGGCAAATCTCTCTACTTGGTATGTTATACAACCGCTTTTTATACTTTGAAATAAATTTTTTTAAAAAATAACCCGGTAGAATATTCGTAAACTTACACCACAGGATACTTAGTGATCAACTAAATAGAAATTTGGACGAGGAATTAAGTCAACTTTAGGAGAAGAAAACGTCCAAATTGGTAACACTCTTGGGTTCACCAAACTTTAAGCCCAATTGATAATCATCTAGTTTTCTTATTTTATTTAATTTTCAAAATTAAATCTATTAAAGCACTTCCTTTCCCTTCTAAATCTATTATCTATTTTTTCTTTTATCAATTTTTAGAAAAACCGCACAAACACTTAAGTGACAATTATTTGTTCATAATTTTTTTTAATGTATGAGAAAGAAGCGTCTCAAATTTAGATCTAGTAATTAAAAAAGGATGTTGCTCCCAGAGATTGAGAGAGAGTAAGAATTGGTTCTTGTTCGCACCGTCTTAAAAGAGTTGGTGGGTCTCCCCACTAAAAACATCAATTTTATACTTTATTAATTTTTTACCTCATAAATTTTAGATGTTAGAAACTCTCCCACTTCATAACACTATGGATTTCACAATTCCATTCCTTGTCCTTGTCCTAAATATTCGAGAGTATGTCTCTGAATCTACGTAAACCATTGTGGGAGGCTACTTGATTAATTTAATAATTTATCGTCCTATAATGAATTAGGGAGGCGACTAGTTTGTCGTACGAAAGTTGATATATATCAAAACATTAAAGACTAAAGCATCCATATATATTTAGCTATATGAGTTTTGTGTCTGTTTGAAAGAAAAGTCATTTCTTCATCCGTACAGAAAACCACAATAAACTCAGTAGCAGAAGGGACAGGAGTGATAACAGCTCAAGCAAGGGAAGATGCCACAGATGAAAGTGGTTTCACATTCGCATATTGCAACATAACAGGAACAGGAGACACTTATTTAGGAAGAGCTTGGAAAGAGAGAACTCGTGTTGTTTTTGCTTACACATACATGGGAACTCTCATCAACACAGAAGGATGGTCTGATAAAATGCATGGAAGCCAACCTAGAAAGTGAGCAATCTAACAGAAAAAATCACACAAATGATCTTATTTATAGTATATATTCGAACGAGCAAACTAATTATTATTTTGATCAATGTTTAACTCCTTGAATACAGGAGTATGTACTACGGCGAGTACAAATGCAAGGGACCTGGCGCGACTCCGTCTGGTCGAGTGAAGTATGCTAGAATACTCTCGGATGTAGAAGCGAAGGCGTTTTTGAGTATGACATACATTCATGGAAACAAATGGCTTCTACCACCCCCTGATCTTTCTCTCTCCCCCTCTAAACCCAATATCTTCCATTAAAATTGAATCAAAATTACTTGAATTGAAAATGCTTAATCAAAATGGAAGTTAAAATACTATCCCAAACTGTGCTCAAAACCATAAAGTAATTGCTATAGTGATAATTAGAAATTAAAGGAAAGGCCAAGGTAGCAAGGCTGTGCTGTGCCTACAAAACTTTATCCCCATCATATTTCCCCATTTAAATGTCTCTCTCTTTAATTCACATAGAATGAATCCCCCCTTTTTTAGTTTGTCCAAAAGCTTTAGTTGATTGTGGTATTAAACTGATCAACAATGTTGCTGTTGTTTTTGTGTAAACTCTTTTTGCTTTCCAACACTAGTAGTTGTGGGTGGTGAAATTTTTTGAAATATACAACCTAATGTGGTTTTAAATTCTTGTATTATCATTGCATTGAAAAAGGTTACTTAAATAGATCACTATGCATGCTTTGGTGCATTGGGATGGAGTGGAAACAATCAAATCTTCAACAACGACTATGGGGATATTGTTCTATTGATTAACATTTGGTTGCTACTTTGGTCGACACAAACTCTCCCTTTTTTGTTAATTGTTCTTATTCTTTTATTGACCTTAAACTAGCACTTGTGCAATGTAACTCATTTGTTCGATGGTATGTAGTACTTTACATACCTACTTATTATGAAGTATATTCTTTCAGAAGAA

Coding sequence (CDS)

ATGTTCCGGCCGACCGTTGCTTCATCTTTTCCGGCGACGGTGGCGTTCGCAATGGGGGTGATGCTCCAACTCATTATACTAATAATTATTCCGTCCACCGTCGTCGTTGAAGCTCACACAAAAACAATTCCGGCAGATTCATCGAAGCTCGACGAATGGATCGGACACAACATGAAAGAATACAACGATCGTAAGACGAACGAGACAGGAATCAAGGCGCTGGACCGGCGGCTGGCGGAGGCGGAGGATTGTGTTCAATTAATCACCGTTAGAAAAGACGGACGAGGAAATTTCTCGACGATCACTGAAGCGATCGATAGCATTCCGTCAGGAAATCGGCGGCGAGTGGTGGTGTGGATCGGAGGTGGGGTTTATAGAGAGAAAATTACGATCGATGCTTCGAAGCCGTTTGTGACTTTGTATGGACAGAAAGGAAAACGGCCAATGATAACGTTCGACGGTACAGCGTCGGAGTTTGGTACCGTTAAGAGCGCGACGGTGGCGGTGGAATCGGATTATTTCGTTGCCGTCAATCTTACGTTTGTGAATTCAGCTCCAATGCCAGAACTAGGAGGAACGGGAGGGCAAGCAGTAGCAATGAGGATATCTGGTGATAAGGCAGCATTCCATGGGTGTCATTTCATAGGCTTTCAAGACACTTTATGTGATGACAGAGGCAGACATTTTTTCAAGGACTGTTACGTCCAAGGCACTGTGGACTTCATCTTTGGAAATGGCAAATCTCTCTACTTGAAAACCACAATAAACTCAGTAGCAGAAGGGACAGGAGTGATAACAGCTCAAGCAAGGGAAGATGCCACAGATGAAAGTGGTTTCACATTCGCATATTGCAACATAACAGGAACAGGAGACACTTATTTAGGAAGAGCTTGGAAAGAGAGAACTCGTGTTGTTTTTGCTTACACATACATGGGAACTCTCATCAACACAGAAGGATGGTCTGATAAAATGCATGGAAGCCAACCTAGAAAGAGTATGTACTACGGCGAGTACAAATGCAAGGGACCTGGCGCGACTCCGTCTGGTCGAGTGAAGTATGCTAGAATACTCTCGGATGTAGAAGCGAAGGCGTTTTTGAGTATGACATACATTCATGGAAACAAATGGCTTCTACCACCCCCTGATCTTTCTCTCTCCCCCTCTAAACCCAATATCTTCCATTAA

Protein sequence

MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKEYNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWIGGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVDFIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKERTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDVEAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH

Homology

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BLAST of Cucsat.G7182 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 359.8 bits (922), Expect = 4.0e-98
Identity = 173/362 (47.79%), Postives = 241/362 (66.57%), Query Frame = 0


Query: 21  MLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKEYNDRKTNETGIKALDRRLAE 80
           ++  I+L++I S VV       IP +  ++++W   N+K+                    
Sbjct: 8   LIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTNVKQ-------------------- 67

Query: 81  AEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWIGGGVYREKITIDASKPFVTL 140
                       +GRG+F TITEAI+S+ +GN RRV++ IG GVY+EK+TID SKPF+TL
Sbjct: 68  ------------NGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITL 127

Query: 141 YGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAM 200
           YG     P++TFDGTA+++GTV SAT+ V SDYF+AVN+   NSAPMP+    G QA++M
Sbjct: 128 YGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSM 187

Query: 201 RISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVDFIFGNGKSLYLKTTINSVAE 260
           RISG+KAAF+ C F G+QDT+CDD G HFFKDCY++GT DFIFG+G+SLYL T +N V +
Sbjct: 188 RISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGD 247

Query: 261 GTGVITAQAREDATDESGFTFAYCNITGTG-DTYLGRAWKERTRVVFAYTYMGTLINTEG 320
           G  VITA A + A ++SG++F +C +TGTG   YLGR+W    +VV+AYT M +++N  G
Sbjct: 248 GIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNPSG 307

Query: 321 WSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDVEAKAFLSMTYIHGNKWLLP 380
           W +     +  K+++YGEYKC G G+    RVKY + + D+EAK F+S+ YI G+ WLLP
Sbjct: 308 WQENREAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLP 336

Query: 381 PP 382
           PP
Sbjct: 368 PP 336

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BLAST of Cucsat.G7182 vs. ExPASy Swiss-Prot
Match: Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.2e-97
Identity = 174/362 (48.07%), Postives = 247/362 (68.23%), Query Frame = 0


Query: 21  MLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKEYNDRKTNETGIKALDRRLAE 80
           M  +  L++  S VV+   T  IP + +++ +W   N+K Y+ RK        LD  L  
Sbjct: 6   MSVVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRK------GTLDPALEA 65

Query: 81  AEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWIGGGVYREKITIDASKPFVTL 140
           AE   Q+ITV + G  NF T+ EAI SIP+GN+ RV++ +  GVY EK+TID ++PF+TL
Sbjct: 66  AEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITL 125

Query: 141 YGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAM 200
            GQ G   ++T+ GTA+++GTV+SAT+ V ++YF A +LT  N+APMP+  G+ GQA+AM
Sbjct: 126 LGQPGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPK-PGSQGQALAM 185

Query: 201 RISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVDFIFGNGKSLYLKTTINSVAE 260
           RI+ DKAAF+ C F GFQDTLCDD+G HFFKDCY++GT DFIFG G SLYL T +++V +
Sbjct: 186 RINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVGD 245

Query: 261 GTGVITAQAREDATDESGFTFAYCNITGTG-DTYLGRAWKERTRVVFAYTYMGTLINTEG 320
           G  VITAQ R+ AT+++G+TF +C +TGTG   YLGR+W    +VV+A+T M +++N  G
Sbjct: 246 GLRVITAQGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNPSG 305

Query: 321 WSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDVEAKAFLSMTYIHGNKWLLP 380
           W + ++     K+++YGEYKC GPG+    RV Y + +   E   FL++ YI G+ WLLP
Sbjct: 306 WRENLNRGYD-KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLP 359

Query: 381 PP 382
           PP
Sbjct: 366 PP 359

[Close]

BLAST of Cucsat.G7182 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 338.2 bits (866), Expect = 1.3e-91
Identity = 170/365 (46.58%), Postives = 239/365 (65.48%), Query Frame = 0


Query: 20  VMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKEYNDRKTNETGIKALDRRLA 79
           V + L +L+I  S +V       IP    ++ +W   ++     R       K LD  L 
Sbjct: 6   VSILLGMLVIFVSPMVFADDLTPIPEGKPQVVQWFNTHVGPLAQR-------KGLDPALV 65

Query: 80  EAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWIGGGVYREKITIDASKPFVT 139
            AE   ++I V   G G F T+T+AI S+P+GN +RV++ +  G YREK+TID +KPF+T
Sbjct: 66  AAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFIT 125

Query: 140 LYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVA 199
           L GQ    P+IT+DGTA+++GTV SA++ + SDYF+AVN+   N+AP P+    G QA++
Sbjct: 126 LMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALS 185

Query: 200 MRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVDFIFGNGKSLYLKTTINSVA 259
           MRISG+ AAF+ C F GFQDT+CDD G HFFKDCYV+GT DFIFG+G S+YL T ++ V 
Sbjct: 186 MRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVG 245

Query: 260 EGTGVITAQAREDATDESGFTFAYCNITGT-GDTYLGRAWKERTRVVFAYTYMGTLINTE 319
           +G  VI A A + A ++SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N  
Sbjct: 246 DGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPT 305

Query: 320 GWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDVEAKAFLSMTYIHGNKWLL 379
           GW +    +   K+++YGEYKC GPG+  + RV + + + D EA  FLS+ YI G+KWLL
Sbjct: 306 GWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLL 361

Query: 380 PPPDL 384
           PPP L
Sbjct: 366 PPPAL 361

[Close]

BLAST of Cucsat.G7182 vs. ExPASy Swiss-Prot
Match: Q9LY18 (Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.3e-91
Identity = 162/357 (45.38%), Postives = 239/357 (66.95%), Query Frame = 0


Query: 26  ILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKEYNDRKTNETGIKALDRRLAEAEDCV 85
           +L+   S VV+      IPAD +++ +W   N+K ++ R+        LD  L  AE   
Sbjct: 11  LLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRR------GTLDPELEAAEASR 70

Query: 86  QLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWIGGGVYREKITIDASKPFVTLYGQKG 145
           ++I V ++G G+F TI  AI SIP  N+ RV++ +  G+Y EK+T+D  +P+VTL G+ G
Sbjct: 71  RVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVTLLGKPG 130

Query: 146 KRPMITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAMRISGD 205
               +T+ GTA+++GTV+SAT+ V +  F+A NL  +N++PMP+  GT GQA+AMRI+GD
Sbjct: 131 AETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPK-PGTQGQALAMRINGD 190

Query: 206 KAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVDFIFGNGKSLYLKTTINSVAEGTGVI 265
           KAAF+ C F GFQDTLCDDRG HFFK+CY++GT DFIFG G SLYL T +++V +G  VI
Sbjct: 191 KAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAVGDGLRVI 250

Query: 266 TAQAREDATDESGFTFAYCNITGTG-DTYLGRAWKERTRVVFAYTYMGTLINTEGWSDKM 325
            A  R+  T+++G++F +C +TG G   YLGRAW    +VV++YT M +++N  GW +  
Sbjct: 251 AAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNPSGWQENR 310

Query: 326 HGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDVEAKAFLSMTYIHGNKWLLPPP 382
             +   K+++YGEY C GPG+  + RV + + + + EA  FL++ YI G+KWLLPPP
Sbjct: 311 VRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLLPPP 359

[Close]

BLAST of Cucsat.G7182 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.6e-91
Identity = 168/367 (45.78%), Postives = 241/367 (65.67%), Query Frame = 0


Query: 18  MGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKEYNDRKTNETGIKALDRR 77
           + ++L L+++ + P  +V       IP    ++ +W   N+     R       K LD  
Sbjct: 6   VSILLGLLMVFVTP--MVFADDVTPIPEGKPQVAQWFNANVGPLAQR-------KGLDPA 65

Query: 78  LAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWIGGGVYREKITIDASKPF 137
           L  AE   ++I V   G G F T+T+AI S+P+GN +RV++ +  G Y+EK+TID +KPF
Sbjct: 66  LVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPF 125

Query: 138 VTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQA 197
           +TL GQ    P+IT+DGTA+++GTV SA++ + SDYF+AVN+   N+AP P+    G QA
Sbjct: 126 ITLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQA 185

Query: 198 VAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVDFIFGNGKSLYLKTTINS 257
           ++MRISG+ AAF+ C F GFQDT+CDD G HFFKDCYV+GT DFIFG+G S+YL T ++ 
Sbjct: 186 LSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHV 245

Query: 258 VAEGTGVITAQAREDATDESGFTFAYCNITGT-GDTYLGRAWKERTRVVFAYTYMGTLIN 317
           V +G  VI A A + A ++SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N
Sbjct: 246 VGDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVN 305

Query: 318 TEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDVEAKAFLSMTYIHGNKW 377
             GW +    +   K+++YGEYKC GPG+  + RV + + + D EA  FLS+ YI G+KW
Sbjct: 306 PTGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKW 361

Query: 378 LLPPPDL 384
           LLPPP L
Sbjct: 366 LLPPPAL 361

[Close]

BLAST of Cucsat.G7182 vs. NCBI nr
Match: XP_004144054.1 (putative pectinesterase 63 [Cucumis sativus] >KGN66331.1 hypothetical protein Csa_007174 [Cucumis sativus])

HSP 1 Score: 799 bits (2064), Expect = 5.12e-292
Identity = 394/394 (100.00%), Postives = 394/394 (100.00%), Query Frame = 0


Query: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
           MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE
Sbjct: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60

Query: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
           YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI
Sbjct: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
           FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
           FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394
           EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394

[Close]

BLAST of Cucsat.G7182 vs. NCBI nr
Match: XP_008450966.1 (PREDICTED: putative pectinesterase 63 [Cucumis melo])

HSP 1 Score: 759 bits (1959), Expect = 5.18e-276
Identity = 373/394 (94.67%), Postives = 382/394 (96.95%), Query Frame = 0


Query: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
           MFRPTVASSF ATVA A+ VM QLIILI IPSTV+VEA TKTIPADSSKLDEWI HNM+E
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
           YNDRK NETGIKALDRRLAEAEDCV+LITVRKDGRGNFSTITEAI+SIPSGNRRRVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGGVYREKITIDASKPFVTLYGQKGKRP+ITFDGTASEFGTVKSATVAVESDYFVAVNLT
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
           FVNSAPMPELGGTGGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFF+DCY+QGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
           FIFGNGKSLYLKTTINSVAEG GVITAQAREDATDESGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGAT SGRVKYARILSDV
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394
           EAKAFLSMTYIHGNKWLLPPPDLS SPSKPNIFH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394

[Close]

BLAST of Cucsat.G7182 vs. NCBI nr
Match: XP_038880021.1 (putative pectinesterase 63 [Benincasa hispida])

HSP 1 Score: 702 bits (1812), Expect = 1.17e-253
Identity = 348/394 (88.32%), Postives = 370/394 (93.91%), Query Frame = 0


Query: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
           M RPTVASS PA VA ++  + QLIILII  S+ VVEA TK IPAD+SKLD+WI HNMKE
Sbjct: 1   MSRPTVASSSPAAVAVSLA-LFQLIILII--SSAVVEARTKMIPADASKLDQWIAHNMKE 60

Query: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
           Y +RK NETGIKALDRRLA+AED V+LITVRKDGRGNF+TIT+AI+SIPSGNRRRVVVWI
Sbjct: 61  YEERKANETGIKALDRRLAKAEDHVRLITVRKDGRGNFTTITKAIESIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGGVYREKITI+ASKPFVTLYGQKG+RP+ITFDGTASEFGTVKSATVAVESDYFVAVNL 
Sbjct: 121 GGGVYREKITIEASKPFVTLYGQKGRRPVITFDGTASEFGTVKSATVAVESDYFVAVNLA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
           FVNSAPMPELGGTGGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCY+QGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEGTGVITAQARE+ATD+SGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTIINSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKC GPGATPSGRVKYARILSD 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDE 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394
           EAKAFLSMTYIHGNKWLLPPP+LSL  S+ N+FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPNLSLFHSRSNVFH 391

[Close]

BLAST of Cucsat.G7182 vs. NCBI nr
Match: KAG6590056.1 (putative pectinesterase 50, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 646 bits (1667), Expect = 1.48e-231
Identity = 315/387 (81.40%), Postives = 348/387 (89.92%), Query Frame = 0


Query: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
           M RP VASSFPATVA  + V+ QLI  I+IP+ +VVEA TKTIPA+ S+LDEWI HNM+E
Sbjct: 1   MARPAVASSFPATVALVLAVV-QLIT-ILIPAAIVVEARTKTIPAEPSQLDEWIAHNMRE 60

Query: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
           Y  RK NETG K LD RLA+AEDC +LITV++DG+GNFSTIT+AIDSIPSGNR RVVVWI
Sbjct: 61  YRQRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIDSIPSGNRERVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGG YREKITID+SKPFVTLYGQ GK P+I++DGTASEFGT +SATVAVESDYFVAVN+ 
Sbjct: 121 GGGTYREKITIDSSKPFVTLYGQIGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFFKDCY++GTVD
Sbjct: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEGTGVITAQARE++TD+SGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA  +GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDD 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSP 387
           EAKAFLSMTYIHGNKW+LPPP LSL P
Sbjct: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 385

[Close]

BLAST of Cucsat.G7182 vs. NCBI nr
Match: XP_022147590.1 (putative pectinesterase 63 [Momordica charantia])

HSP 1 Score: 645 bits (1665), Expect = 2.67e-231
Identity = 319/394 (80.96%), Postives = 350/394 (88.83%), Query Frame = 0


Query: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
           M+RP VASS P  VA A+ V +QLII  I+P+ VV  A TK IPA+ SKLDEWI +NMKE
Sbjct: 1   MYRPAVASSSPVAVAIALAV-IQLII--ILPAAVV--ARTKPIPAEPSKLDEWIENNMKE 60

Query: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
           Y++RK N+TG+KALD+RLA AED V+LITVR DGRGNF+TIT AI+SIP+GN  RV+VWI
Sbjct: 61  YHERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
            GG+YREKITIDASKPFVTLYGQ GK P+ITFDGTASEFGTV+SATVAVESDYFVAVN+ 
Sbjct: 121 AGGIYREKITIDASKPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
           FVNSA MP++  +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCY++GTVD
Sbjct: 181 FVNSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG GVITAQAREDATD+SGFTF YCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
           RTRVVFAYTYMG LINTEGWSDKMH  +PRKSMYYGEYKC GPGATP+GRVKYARILSD 
Sbjct: 301 RTRVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDE 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394
           EA+AFLSMTYIHGNKWLLPPP LS S S PN FH
Sbjct: 361 EARAFLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 389

[Close]

BLAST of Cucsat.G7182 vs. ExPASy TrEMBL
Match: A0A0A0M299 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1)

HSP 1 Score: 799 bits (2064), Expect = 2.48e-292
Identity = 394/394 (100.00%), Postives = 394/394 (100.00%), Query Frame = 0


Query: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
           MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE
Sbjct: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60

Query: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
           YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI
Sbjct: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
           FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
           FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394
           EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394

[Close]

BLAST of Cucsat.G7182 vs. ExPASy TrEMBL
Match: A0A1S3BRI5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1)

HSP 1 Score: 759 bits (1959), Expect = 2.51e-276
Identity = 373/394 (94.67%), Postives = 382/394 (96.95%), Query Frame = 0


Query: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
           MFRPTVASSF ATVA A+ VM QLIILI IPSTV+VEA TKTIPADSSKLDEWI HNM+E
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
           YNDRK NETGIKALDRRLAEAEDCV+LITVRKDGRGNFSTITEAI+SIPSGNRRRVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGGVYREKITIDASKPFVTLYGQKGKRP+ITFDGTASEFGTVKSATVAVESDYFVAVNLT
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
           FVNSAPMPELGGTGGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFF+DCY+QGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
           FIFGNGKSLYLKTTINSVAEG GVITAQAREDATDESGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGAT SGRVKYARILSDV
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394
           EAKAFLSMTYIHGNKWLLPPPDLS SPSKPNIFH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394

[Close]

BLAST of Cucsat.G7182 vs. ExPASy TrEMBL
Match: A0A6J1D0K0 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1)

HSP 1 Score: 645 bits (1665), Expect = 1.29e-231
Identity = 319/394 (80.96%), Postives = 350/394 (88.83%), Query Frame = 0


Query: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
           M+RP VASS P  VA A+ V +QLII  I+P+ VV  A TK IPA+ SKLDEWI +NMKE
Sbjct: 1   MYRPAVASSSPVAVAIALAV-IQLII--ILPAAVV--ARTKPIPAEPSKLDEWIENNMKE 60

Query: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
           Y++RK N+TG+KALD+RLA AED V+LITVR DGRGNF+TIT AI+SIP+GN  RV+VWI
Sbjct: 61  YHERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
            GG+YREKITIDASKPFVTLYGQ GK P+ITFDGTASEFGTV+SATVAVESDYFVAVN+ 
Sbjct: 121 AGGIYREKITIDASKPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
           FVNSA MP++  +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCY++GTVD
Sbjct: 181 FVNSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG GVITAQAREDATD+SGFTF YCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
           RTRVVFAYTYMG LINTEGWSDKMH  +PRKSMYYGEYKC GPGATP+GRVKYARILSD 
Sbjct: 301 RTRVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDE 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394
           EA+AFLSMTYIHGNKWLLPPP LS S S PN FH
Sbjct: 361 EARAFLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 389

[Close]

BLAST of Cucsat.G7182 vs. ExPASy TrEMBL
Match: A0A6J1HB45 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1)

HSP 1 Score: 645 bits (1665), Expect = 1.44e-231
Identity = 314/387 (81.14%), Postives = 348/387 (89.92%), Query Frame = 0


Query: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
           M RP VASSFPATVA  + V+ QLI  I+IP+ +VVEA TKTIPA+ S+LDEWI HNM+E
Sbjct: 1   MSRPAVASSFPATVALVLAVV-QLIT-ILIPAAIVVEARTKTIPAEPSQLDEWIAHNMRE 60

Query: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
           Y  RK NETG K LD RLA+AEDC +LITV++DG+GNFSTIT+AI+SIPSGNR RVVVWI
Sbjct: 61  YRQRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNRERVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGG YREKITID+SKPFVTLYGQ GK P+I++DGTASEFGT +SATVAVESDYFVAVN+ 
Sbjct: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFFKDCY++GTVD
Sbjct: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEGTGVITAQARE++TD+SGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA  +GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDD 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSP 387
           EAKAFLSMTYIHGNKW+LPPP LSL P
Sbjct: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 385

[Close]

BLAST of Cucsat.G7182 vs. ExPASy TrEMBL
Match: A0A6J1JI92 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1)

HSP 1 Score: 645 bits (1663), Expect = 2.91e-231
Identity = 314/387 (81.14%), Postives = 348/387 (89.92%), Query Frame = 0


Query: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60
           M RP VASSFPATVA  + V+ QLI ++I P+ +VVEA TK IPA+ S+LDEWI HNM+E
Sbjct: 1   MSRPAVASSFPATVALVLAVV-QLITMLI-PAAIVVEARTKPIPAEPSQLDEWIAHNMRE 60

Query: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120
           Y  RK  ETG KALDRRLA+AEDC +LITV++DG+GNFSTIT+AI+SIPSGN  RVVVWI
Sbjct: 61  YRQRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGG YREKITID+SKPFVTLYGQ GK P+I++DGTASEFGTV+SATVAVESDYFVAVN+ 
Sbjct: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFFKDCY++GTVD
Sbjct: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEGTGVITAQARE++TDESGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA  +GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDD 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSP 387
           EAKAFLSMTYIHGNKW+LPPP LSL P
Sbjct: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 385

The following BLAST results are available for this feature:

BLAST of Cucsat.G7182 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FKF3	4.0e-98	47.79	Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2	[more]
Q9LY17	1.2e-97	48.07	Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1	[more]
Q9LY19	1.3e-91	46.58	Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2	[more]
Q9LY18	1.3e-91	45.38	Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1	[more]
Q84WM7	1.6e-91	45.78	Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1	[more]

BLAST of Cucsat.G7182 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144054.1	5.12e-292	100.00	putative pectinesterase 63 [Cucumis sativus] >KGN66331.1 hypothetical protein Cs...	[more]
XP_008450966.1	5.18e-276	94.67	PREDICTED: putative pectinesterase 63 [Cucumis melo]	[more]
XP_038880021.1	1.17e-253	88.32	putative pectinesterase 63 [Benincasa hispida]	[more]
KAG6590056.1	1.48e-231	81.40	putative pectinesterase 50, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022147590.1	2.67e-231	80.96	putative pectinesterase 63 [Momordica charantia]	[more]

BLAST of Cucsat.G7182 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M299	2.48e-292	100.00	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1	[more]
A0A1S3BRI5	2.51e-276	94.67	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1	[more]
A0A6J1D0K0	1.29e-231	80.96	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1	[more]
A0A6J1HB45	1.44e-231	81.14	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1	[more]
A0A6J1JI92	2.91e-231	81.14	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 83..379 e-value: 2.9E-103 score: 347.6
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 88..374 e-value: 6.7E-70 score: 235.1
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 20..382
None	No IPR available	PANTHER	PTHR31321:SF30	PECTINESTERASE	coord: 20..382
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 235..244
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 88..377

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G7182.T1	Cucsat.G7182.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity