Cucsat.G6733 (gene) Cucumber (B10) v3

Overview
NameCucsat.G6733
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionGDSL esterase/lipase
Locationctg1502: 884153 .. 885771 (-)
RNA-Seq ExpressionCucsat.G6733
SyntenyCucsat.G6733
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCATCGCCTCTCTCCAACTTATTCAAACACACCGCCATTCATAATAAGCATTCATCAATGGCCGCTTCTACCAAATCTTCTGTCTCTCTTTTTCTTCTTCTTCTCGTTCTTGTTTTGGGTTCATCCAAAGCTCATCCCCTTAAGGCTTGTATGTTCGATGCTATCTACCAACTCGGCGACTCAATCTCCGACACCGGAAATCTCATTCGTGAAAACCTCAACACTCCCTTTTCTCATCTTCCTTATGGCCAATCTTTCTTCAACAATCCCACCGGTCGATGCTCCAACGGTCTACTTATGCTTGATTTCTTGGGTAAGCATGACCTTCCATATATATATATTCTACACATGTTTTTATATCTCGCAAACTAATAATATAACTATGACTCAATGTATTTGTAGCTTTGGATGCCGGACTTCCTTTGGTGAGTCCTTATTTGAATAAAGATGGTTTGATGGATCATGGAGTCAACTTTGCCGTGGCTGGTTCTACAGCTTTGCCTTCCCAATATCTTTCTTCAAGTTACAAAATCATATCTCCAGTTACCAACTCTTCTCTCGACCATCAACTTGATTGGATGTTCTCTCATTTCAACTCTATTTGTCACAATCAAAGAGGTGACTAATTATTAATTAGCATACTAGGCTACTTACATTATTTAATATTTATGCTGCCATGATGTAATTTATATATATTTATGCATTTTAATTGCCGTGTAGAATGCAACGAGAAATTAAGGAGTGCTTTGTTCTTGGTCGGTGAGATTGGTGGCAATGACTATAACTATGCATTATTCCAAGGCAAAACTATTCAAGAGGCAAAAGACATGGTACCAGATGTTGTTCAAACCATTAAGAGTGCCGTTGAGGTAATGTTAACTTTGTTTGACACAATAATAGATTAGATCATATATATTCCATATATGAATGAGATGATTATTTACTTAATTGCCTCTCTTTTGCTTTGTAACCTTTGTAGAAAGTAATAAGCTACGGTGCAACACGAGTTGTTGTTCCTGGAAATTTTCCTATTGGATGCTTCCCTATCTATCTCACTGGATTCCATACTAATGACACATCCGCTTACGATGAGCTTCACTGCTTAAAGGATTTGAATAGTTTTGCAACCTATCATAACGATCAAATCAAGCAAGCCATTGAAGTACTCAAAAAAGAGAATCCACATGCCATTATCGTATATGGTGATTATTACAATGCATTTCTATGGATTATTCGTCATGCTTTCGTTCTAGGTATGTGCCTAATTTTTCTACTCTTAATTACAGTGACATTTCTCAATCTAATTTTAATTTAATTTTTGTACTTAATTACTTATATATGTATAAATAAATTAAACAGGATTTGATGAAGAATCTTTGCAAAAATCATGCTGTGGGATTGGAGGAGATTACAAGTTTAACCTAATGCAAATGTGTGGAGTAGCTGGAGTTGAAGCTTGCCCAAATCCTAATGAACATATCAGTTGGGATGGAGTCCATTTGACACAGAAGACTTACAAGTTCATGACACATTGGCTCATCCATGACATTTTTCCTAAATTGCATTGCATTGTTTAATTTTTTTAAAATTATGTATATGGTGCTATTTTTTATTCTTTT

Coding sequence (CDS)

TCATCGCCTCTCTCCAACTTATTCAAACACACCGCCATTCATAATAAGCATTCATCAATGGCCGCTTCTACCAAATCTTCTGTCTCTCTTTTTCTTCTTCTTCTCGTTCTTGTTTTGGGTTCATCCAAAGCTCATCCCCTTAAGGCTTGTATGTTCGATGCTATCTACCAACTCGGCGACTCAATCTCCGACACCGGAAATCTCATTCGTGAAAACCTCAACACTCCCTTTTCTCATCTTCCTTATGGCCAATCTTTCTTCAACAATCCCACCGGTCGATGCTCCAACGGTCTACTTATGCTTGATTTCTTGGCTTTGGATGCCGGACTTCCTTTGGTGAGTCCTTATTTGAATAAAGATGGTTTGATGGATCATGGAGTCAACTTTGCCGTGGCTGGTTCTACAGCTTTGCCTTCCCAATATCTTTCTTCAAGTTACAAAATCATATCTCCAGTTACCAACTCTTCTCTCGACCATCAACTTGATTGGATGTTCTCTCATTTCAACTCTATTTGTCACAATCAAAGAGAATGCAACGAGAAATTAAGGAGTGCTTTGTTCTTGGTCGGTGAGATTGGTGGCAATGACTATAACTATGCATTATTCCAAGGCAAAACTATTCAAGAGGCAAAAGACATGGTACCAGATGTTGTTCAAACCATTAAGAGTGCCGTTGAGAAAGTAATAAGCTACGGTGCAACACGAGTTGTTGTTCCTGGAAATTTTCCTATTGGATGCTTCCCTATCTATCTCACTGGATTCCATACTAATGACACATCCGCTTACGATGAGCTTCACTGCTTAAAGGATTTGAATAGTTTTGCAACCTATCATAACGATCAAATCAAGCAAGCCATTGAAGTACTCAAAAAAGAGAATCCACATGCCATTATCGTATATGGTGATTATTACAATGCATTTCTATGGATTATTCGTCATGCTTTCGTTCTAGGATTTGATGAAGAATCTTTGCAAAAATCATGCTGTGGGATTGGAGGAGATTACAAGTTTAACCTAATGCAAATGTGTGGAGTAGCTGGAGTTGAAGCTTGCCCAAATCCTAATGAACATATCAGTTGGGATGGAGTCCATTTGACACAGAAGACTTACAAGTTCATGACACATTGGCTCATCCATGACATTTTTCCTAAATTGCATTGCATTGTTTAA

Protein sequence

SSPLSNLFKHTAIHNKHSSMAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV
Homology
BLAST of Cucsat.G6733 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 6.2e-83
Identity = 151/341 (44.28%), Postives = 214/341 (62.76%), Query Frame = 0

Query: 50  CMFDAIYQLGDSISDTGNLIRENLNTP---FSHLPYGQSFFNNPTGRCSNGLLMLDFLAL 109
           C FD+IYQLGDS SDTGNLIR   + P    +H PYG++F   PTGRCS+G L++DF+A 
Sbjct: 25  CPFDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGETFPGTPTGRCSDGRLIIDFIAT 84

Query: 110 DAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFS 169
              LPL++PYL ++    HGVNFAVAG+TAL   +L++    +S + +S L  QL+W  +
Sbjct: 85  ALNLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLAARGVQVSDI-HSHLSAQLNWFRT 144

Query: 170 HFNSICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVE 229
           +  SIC   +EC+ KL++ALF++G IG ND NYA F  +TI+E +  VP + + + +A  
Sbjct: 145 YLGSICSTPKECSNKLKNALFILGNIGNNDVNYA-FPNRTIEEIRAYVPFITEAVANATR 204

Query: 230 KVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFATYHNDQIKQAI 289
           ++I  G +RV+VPG FPIGC    L   +       D+L CL  LN+ + Y N   ++A+
Sbjct: 205 EIIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDDLGCLSSLNNLSIYFNSLFQRAL 264

Query: 290 EVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGDYKFNLMQMCGVA 349
             L  E P A+I+Y DYYNA+ ++ R+   LG +  SL K CCGIGG Y ++  + CG  
Sbjct: 265 ASLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSLLKCCCGIGGPYNYDPDRECGSR 324

Query: 350 GVEACPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHC 388
           GV  CPNP ++I WDG H TQ  Y+ +  ++I  I   L C
Sbjct: 325 GVPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKALKC 363

BLAST of Cucsat.G6733 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.3e-76
Identity = 159/361 (44.04%), Postives = 203/361 (56.23%), Query Frame = 0

Query: 27  SVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGQSF 86
           S +  L LLV +L  S  H    C  ++IYQ GDSISDTGNLIR                
Sbjct: 2   STTKALSLLVFILFVSLVHSSDQCPINSIYQFGDSISDTGNLIR---------------- 61

Query: 87  FNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSY 146
            N P                 A  P   P   ++   +  VNF V+GSTAL S + S   
Sbjct: 62  -NGP-----------------ASSPTPKPLPQRE--HNVFVNFGVSGSTALNSSFFSER- 121

Query: 147 KIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKT 206
            +  P TN+ L  QL W   H  S CH     ++ L+ +LF+VGEIGGNDYNY  FQGK 
Sbjct: 122 NLHVPATNTPLSMQLAWFKGHLRSTCHG--SSSDCLKHSLFMVGEIGGNDYNYGFFQGKP 181

Query: 207 IQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELH 266
           ++E +  +P VV  I +A  +VI  GA  VVVPGNFP+GCFPIYLT F   DT  YD+  
Sbjct: 182 MEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDYDDNG 241

Query: 267 CLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGFDEESLQK 326
           CL  LN FA  HN+Q+++AI  L+KE P   IVYGDYYNAF +++R      FD+    K
Sbjct: 242 CLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKSVALK 301

Query: 327 SCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLH 386
           SCCG GG Y ++  +  G  GV  C NP++ ISWDGVHLTQK Y+FM+ +L + I  ++ 
Sbjct: 302 SCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQILSQIK 320

Query: 387 C 388
           C
Sbjct: 362 C 320

BLAST of Cucsat.G6733 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 8.1e-75
Identity = 147/365 (40.27%), Postives = 218/365 (59.73%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLI---RENLNTP 79
           MA+     + LF  L V ++ S    P     F +I   GDSI+DTGNL+     N    
Sbjct: 1   MASLDSLVIFLFSTLFVTIVSSETPCP----NFKSIISFGDSIADTGNLVGLSDRNQLPV 60

Query: 80  FSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYL-NKDGLMDHGVNFAVAGST 139
            +  PYG++FF++PTGR  +G +++DF+A   GLP V PY  +K+   D GVNFAVAG+T
Sbjct: 61  TAFPPYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGAT 120

Query: 140 ALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGN 199
           AL S +L    + I P TN SL  QL        ++C +  +C + + +AL L+GEIGGN
Sbjct: 121 ALKSSFLKK--RGIQPHTNVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNALILMGEIGGN 180

Query: 200 DYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFH 259
           DYN+  F  K ++E +++VP V+ +I S + ++I  G    +VPG FPIGC  +YLT + 
Sbjct: 181 DYNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYK 240

Query: 260 TNDTSAYD-ELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHA 319
           T++   YD    CLK LN F  YH++++K  +  L+K  PH  I+Y DYYN+ L I +  
Sbjct: 241 TSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEP 300

Query: 320 FVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMT 379
              GF E     +CCGIGG Y FN  + CG  GV++C +P++++ WDGVH+T+  YK++ 
Sbjct: 301 AKFGFMERPF-PACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIA 358

BLAST of Cucsat.G6733 vs. ExPASy Swiss-Prot
Match: Q9C857 (GDSL esterase/lipase At1g31550 OS=Arabidopsis thaliana OX=3702 GN=At1g31550 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.4e-74
Identity = 151/355 (42.54%), Postives = 219/355 (61.69%), Query Frame = 0

Query: 29  SLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNL--IRENLNTPFSHL-PYGQS 88
           SLFL  L + + SS++   +   F++I   GDSI+DTGNL  + ++ N P S   PYG++
Sbjct: 14  SLFLSTLFVSIVSSES---QCRNFESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGET 73

Query: 89  FFNNPTGRCSNGLLMLDFLALDAGLPLVSPYL-NKDGLMDHGVNFAVAGSTALPSQYLSS 148
           FF++PTGR S+G L++DF+A   GLP V PY  + +G  + GVNFAVA +TAL S +L  
Sbjct: 74  FFHHPTGRFSDGRLIIDFIAEFLGLPYVPPYFGSTNGNFEKGVNFAVASATALESSFLEE 133

Query: 149 SYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNYALFQG 208
             K      N SL  QL        ++C    +C + + +AL L+GEIG NDYN+  FQ 
Sbjct: 134 --KGYHCPHNFSLGVQLKIFKQSLPNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQL 193

Query: 209 KTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDE 268
           + + E K++VP V+ TI SA+ ++I  G    +VPG FP+GC   +LT   T++   YD 
Sbjct: 194 RPLDEVKELVPLVISTISSAITELIGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDP 253

Query: 269 L-HCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGFDEES 328
           L  CLK LN F  YH++Q+++ +  L+K NPH  I+Y DYYNA L + R     GF    
Sbjct: 254 LTGCLKWLNKFGEYHSEQLQEELNRLRKLNPHVNIIYADYYNASLRLGREPSKYGFINRH 313

Query: 329 LQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLI 379
           L  +CCG+GG Y FNL + CG  GVEAC +P+++++WDG+H+T+  +K M   L+
Sbjct: 314 L-SACCGVGGPYNFNLSRSCGSVGVEACSDPSKYVAWDGLHMTEAAHKSMADGLV 362

BLAST of Cucsat.G6733 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.5e-73
Identity = 149/364 (40.93%), Postives = 218/364 (59.89%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNL--IRENLNTP- 79
           MA      VS FL+L    L +  + P +   F +I   GDSI+DTGNL  + +  N P 
Sbjct: 1   MATLFMKLVSFFLILSTFCLTTVNSEP-QCHNFKSIISFGDSIADTGNLLALSDPTNLPK 60

Query: 80  FSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSP-YLNKDGLMDHGVNFAVAGST 139
            + LPYG++FF++PTGR SNG L++DF+A   G PLV P Y +++   + GVNFAV G+T
Sbjct: 61  VAFLPYGETFFHHPTGRFSNGRLIIDFIAEFLGFPLVPPFYGSQNANFEKGVNFAVGGAT 120

Query: 140 ALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGN 199
           AL   +L     I  P TN SL  QL        ++C +  +C + + ++L L+GEIGGN
Sbjct: 121 ALERSFLEER-GIHFPYTNVSLAVQLSSFKESLPNLCVSPSDCRDMIENSLILMGEIGGN 180

Query: 200 DYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFH 259
           DYNYA F GK I+E K++VP V++TI SA+ ++I  G    +VPG FP+GC   YL+ + 
Sbjct: 181 DYNYAFFVGKNIEEIKELVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQ 240

Query: 260 TNDTSAYDEL-HCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHA 319
           T++   YD L  CLK LN F+ YH++Q++  +  L+K  PH  I+Y DYYN  L + +  
Sbjct: 241 TSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEP 300

Query: 320 FVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMT 379
              GF    L  +CC +GG + F L +  G    E C +P++++SWDGVH+T+  Y+ M 
Sbjct: 301 AKFGFISRPL-PACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLMA 360

BLAST of Cucsat.G6733 vs. NCBI nr
Match: XP_011659746.1 (acetylajmalan esterase-like [Cucumis sativus])

HSP 1 Score: 760 bits (1963), Expect = 4.20e-277
Identity = 366/370 (98.92%), Postives = 368/370 (99.46%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSH 79
           MA STKSS+SLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIREN NTPFSH
Sbjct: 1   MAPSTKSSLSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 80  LPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPS 139
           LPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPS
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPS 120

Query: 140 QYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNY 199
           QYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNY
Sbjct: 121 QYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNY 180

Query: 200 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 259
           ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT
Sbjct: 181 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 240

Query: 260 SAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGF 319
           SAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHA+IVYGDYYNAFLWIIRHAFVLGF
Sbjct: 241 SAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAVIVYGDYYNAFLWIIRHAFVLGF 300

Query: 320 DEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 379
           DEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH
Sbjct: 301 DEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 360

Query: 380 DIFPKLHCIV 389
           DIFPKLHCIV
Sbjct: 361 DIFPKLHCIV 370

BLAST of Cucsat.G6733 vs. NCBI nr
Match: XP_016903430.1 (PREDICTED: acetylajmalan esterase-like [Cucumis melo])

HSP 1 Score: 733 bits (1891), Expect = 3.96e-266
Identity = 350/370 (94.59%), Postives = 357/370 (96.49%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSH 79
           M ASTKSS+SLFLLL VLVLGSSKAHPLKA MFDAIYQLGDSISDTGNLIREN NTPFSH
Sbjct: 1   MGASTKSSLSLFLLLFVLVLGSSKAHPLKAFMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 80  LPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPS 139
           LPYGQSFFNNPTGRCSNGLLMLDF ALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTAL S
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSS 120

Query: 140 QYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNY 199
           QYLS+ YKI+SPVTNSSLDHQL+WMFSHFNSICHNQR+CNEKLRSALF VGEIGGNDYNY
Sbjct: 121 QYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNY 180

Query: 200 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 259
           ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT
Sbjct: 181 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 240

Query: 260 SAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGF 319
           SAYDELHCLKDLN FATYHNDQIKQ IEVLKKENPH +IVYGDYYNAFLWIIRHA VLGF
Sbjct: 241 SAYDELHCLKDLNGFATYHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLGF 300

Query: 320 DEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 379
           DE+SLQKSCCGIGGDY FNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH
Sbjct: 301 DEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 360

Query: 380 DIFPKLHCIV 389
           DIFPKLHCIV
Sbjct: 361 DIFPKLHCIV 370

BLAST of Cucsat.G6733 vs. NCBI nr
Match: TYK13517.1 (acetylajmalan esterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 731 bits (1886), Expect = 2.29e-265
Identity = 349/370 (94.32%), Postives = 357/370 (96.49%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSH 79
           M ASTKSS+SLFLLL VLVLGSSKAHPLKA MFDAIYQLGDSISDTGNLIREN NTPFSH
Sbjct: 1   MGASTKSSLSLFLLLFVLVLGSSKAHPLKAFMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 80  LPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPS 139
           LPYGQSFFNNPTGRCSNGLLMLDF ALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTAL S
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSS 120

Query: 140 QYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNY 199
           QYLS+ YKI+SPVTNSSLDHQL+WMFSHFNSICHNQR+CNEKLRSALF VGEIGGNDYNY
Sbjct: 121 QYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNY 180

Query: 200 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 259
           ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT
Sbjct: 181 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 240

Query: 260 SAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGF 319
           SAYDELHCLKDLN FAT+HNDQIKQ IEVLKKENPH +IVYGDYYNAFLWIIRHA VLGF
Sbjct: 241 SAYDELHCLKDLNGFATHHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLGF 300

Query: 320 DEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 379
           DE+SLQKSCCGIGGDY FNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH
Sbjct: 301 DEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 360

Query: 380 DIFPKLHCIV 389
           DIFPKLHCIV
Sbjct: 361 DIFPKLHCIV 370

BLAST of Cucsat.G6733 vs. NCBI nr
Match: KAA0032295.1 (acetylajmalan esterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 729 bits (1883), Expect = 6.57e-265
Identity = 349/370 (94.32%), Postives = 356/370 (96.22%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSH 79
           M ASTKSS+SLFLLL VLVLGSSKAHPLKA MFDAIYQLGDSISDTGNLIREN NTPFSH
Sbjct: 1   MGASTKSSLSLFLLLFVLVLGSSKAHPLKAFMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 80  LPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPS 139
           LPYGQSFFNNPTGRCSNGLLMLDF ALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTAL S
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSS 120

Query: 140 QYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNY 199
           QYLS+ YKI+SPVTNSSLDHQL+WMFSHFNSICHNQR+CNEKLRSALF VGEIGGNDYNY
Sbjct: 121 QYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNY 180

Query: 200 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 259
           ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT
Sbjct: 181 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 240

Query: 260 SAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGF 319
           SAYDELHCLKDLN FATYHNDQIKQ IEVLKKENPH +IVYGDYYNAFLWIIRHA VLGF
Sbjct: 241 SAYDELHCLKDLNGFATYHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLGF 300

Query: 320 DEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 379
           DE+SLQKSCCGIGGDY FNLMQMCGVAGVEACPNPNEHISWD VHLTQKTYKFMTHWLIH
Sbjct: 301 DEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDRVHLTQKTYKFMTHWLIH 360

Query: 380 DIFPKLHCIV 389
           DIFPKLHCIV
Sbjct: 361 DIFPKLHCIV 370

BLAST of Cucsat.G6733 vs. NCBI nr
Match: XP_031737213.1 (acetylajmalan esterase-like [Cucumis sativus])

HSP 1 Score: 720 bits (1859), Expect = 3.10e-261
Identity = 341/370 (92.16%), Postives = 356/370 (96.22%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSH 79
           MAASTKSS SLFLLLL++VLGSSKAHPLKACMFDAIYQLGDSISDTGNLIREN NTPFSH
Sbjct: 1   MAASTKSSFSLFLLLLLVVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 80  LPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPS 139
           LPYGQSFFNNPTGRCSNGLLMLDF ALDAGLPLV+PYLNKDG MDHGVNFAVAGSTALPS
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVTPYLNKDGWMDHGVNFAVAGSTALPS 120

Query: 140 QYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNY 199
           QYLS++YKI+SPVTNSSLDHQL+WMFSHFNSICH+QR+CNEKLRSALFLVGEIGGNDYNY
Sbjct: 121 QYLSTNYKILSPVTNSSLDHQLEWMFSHFNSICHDQRDCNEKLRSALFLVGEIGGNDYNY 180

Query: 200 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 259
           ALFQGKTIQEAK MVPDVV+TIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT
Sbjct: 181 ALFQGKTIQEAKHMVPDVVRTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 240

Query: 260 SAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGF 319
           SAYDELHCLKDLN  ATYHNDQIKQ IEVLKKENP  +IVYGDYYNAFLW+IRHAFVLG+
Sbjct: 241 SAYDELHCLKDLNGLATYHNDQIKQTIEVLKKENPQTVIVYGDYYNAFLWVIRHAFVLGY 300

Query: 320 DEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 379
           DEESLQKSCCGIGGDYKFNLM+MCG AGVEACPNPNEHISWDGVHLTQ TYKFMTHWLIH
Sbjct: 301 DEESLQKSCCGIGGDYKFNLMKMCGAAGVEACPNPNEHISWDGVHLTQNTYKFMTHWLIH 360

Query: 380 DIFPKLHCIV 389
            IFPKLHC +
Sbjct: 361 HIFPKLHCSI 370

BLAST of Cucsat.G6733 vs. ExPASy TrEMBL
Match: A0A1S4E629 (acetylajmalan esterase-like OS=Cucumis melo OX=3656 GN=LOC103503259 PE=3 SV=1)

HSP 1 Score: 733 bits (1891), Expect = 1.92e-266
Identity = 350/370 (94.59%), Postives = 357/370 (96.49%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSH 79
           M ASTKSS+SLFLLL VLVLGSSKAHPLKA MFDAIYQLGDSISDTGNLIREN NTPFSH
Sbjct: 1   MGASTKSSLSLFLLLFVLVLGSSKAHPLKAFMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 80  LPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPS 139
           LPYGQSFFNNPTGRCSNGLLMLDF ALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTAL S
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSS 120

Query: 140 QYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNY 199
           QYLS+ YKI+SPVTNSSLDHQL+WMFSHFNSICHNQR+CNEKLRSALF VGEIGGNDYNY
Sbjct: 121 QYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNY 180

Query: 200 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 259
           ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT
Sbjct: 181 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 240

Query: 260 SAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGF 319
           SAYDELHCLKDLN FATYHNDQIKQ IEVLKKENPH +IVYGDYYNAFLWIIRHA VLGF
Sbjct: 241 SAYDELHCLKDLNGFATYHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLGF 300

Query: 320 DEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 379
           DE+SLQKSCCGIGGDY FNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH
Sbjct: 301 DEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 360

Query: 380 DIFPKLHCIV 389
           DIFPKLHCIV
Sbjct: 361 DIFPKLHCIV 370

BLAST of Cucsat.G6733 vs. ExPASy TrEMBL
Match: A0A5D3CNR6 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold745G00130 PE=3 SV=1)

HSP 1 Score: 731 bits (1886), Expect = 1.11e-265
Identity = 349/370 (94.32%), Postives = 357/370 (96.49%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSH 79
           M ASTKSS+SLFLLL VLVLGSSKAHPLKA MFDAIYQLGDSISDTGNLIREN NTPFSH
Sbjct: 1   MGASTKSSLSLFLLLFVLVLGSSKAHPLKAFMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 80  LPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPS 139
           LPYGQSFFNNPTGRCSNGLLMLDF ALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTAL S
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSS 120

Query: 140 QYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNY 199
           QYLS+ YKI+SPVTNSSLDHQL+WMFSHFNSICHNQR+CNEKLRSALF VGEIGGNDYNY
Sbjct: 121 QYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNY 180

Query: 200 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 259
           ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT
Sbjct: 181 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 240

Query: 260 SAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGF 319
           SAYDELHCLKDLN FAT+HNDQIKQ IEVLKKENPH +IVYGDYYNAFLWIIRHA VLGF
Sbjct: 241 SAYDELHCLKDLNGFATHHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLGF 300

Query: 320 DEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 379
           DE+SLQKSCCGIGGDY FNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH
Sbjct: 301 DEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 360

Query: 380 DIFPKLHCIV 389
           DIFPKLHCIV
Sbjct: 361 DIFPKLHCIV 370

BLAST of Cucsat.G6733 vs. ExPASy TrEMBL
Match: A0A5A7SSW2 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold219G001390 PE=3 SV=1)

HSP 1 Score: 729 bits (1883), Expect = 3.18e-265
Identity = 349/370 (94.32%), Postives = 356/370 (96.22%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSH 79
           M ASTKSS+SLFLLL VLVLGSSKAHPLKA MFDAIYQLGDSISDTGNLIREN NTPFSH
Sbjct: 1   MGASTKSSLSLFLLLFVLVLGSSKAHPLKAFMFDAIYQLGDSISDTGNLIRENPNTPFSH 60

Query: 80  LPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPS 139
           LPYGQSFFNNPTGRCSNGLLMLDF ALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTAL S
Sbjct: 61  LPYGQSFFNNPTGRCSNGLLMLDFFALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALSS 120

Query: 140 QYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNY 199
           QYLS+ YKI+SPVTNSSLDHQL+WMFSHFNSICHNQR+CNEKLRSALF VGEIGGNDYNY
Sbjct: 121 QYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKLRSALFFVGEIGGNDYNY 180

Query: 200 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 259
           ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT
Sbjct: 181 ALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDT 240

Query: 260 SAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGF 319
           SAYDELHCLKDLN FATYHNDQIKQ IEVLKKENPH +IVYGDYYNAFLWIIRHA VLGF
Sbjct: 241 SAYDELHCLKDLNGFATYHNDQIKQTIEVLKKENPHTVIVYGDYYNAFLWIIRHALVLGF 300

Query: 320 DEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 379
           DE+SLQKSCCGIGGDY FNLMQMCGVAGVEACPNPNEHISWD VHLTQKTYKFMTHWLIH
Sbjct: 301 DEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDRVHLTQKTYKFMTHWLIH 360

Query: 380 DIFPKLHCIV 389
           DIFPKLHCIV
Sbjct: 361 DIFPKLHCIV 370

BLAST of Cucsat.G6733 vs. ExPASy TrEMBL
Match: A0A5A7VJA5 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00480 PE=3 SV=1)

HSP 1 Score: 678 bits (1749), Expect = 2.62e-245
Identity = 318/339 (93.81%), Postives = 327/339 (96.46%), Query Frame = 0

Query: 51  MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGL 110
           MFDAIYQLGDSISDTGNLIREN NTPFSHLPYGQSFFNNPTGRCSNGLLMLDF ALDAGL
Sbjct: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 60

Query: 111 PLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNS 170
            LVSPYLNKDG MDHGVNFAVAGSTALPSQYL ++YKI+SPVTNSSLDHQLDWMFSHFNS
Sbjct: 61  SLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNSSLDHQLDWMFSHFNS 120

Query: 171 ICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 230
           ICHNQR+CNEKLRSALFLVGEIGGNDYNYALFQGKTI+EAKDMVPDVVQTIKSAVEKVIS
Sbjct: 121 ICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVPDVVQTIKSAVEKVIS 180

Query: 231 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFATYHNDQIKQAIEVLK 290
           YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLN  ATYHN+QIKQ IEVLK
Sbjct: 181 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLATYHNNQIKQTIEVLK 240

Query: 291 KENPHAIIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEA 350
           KENPH +IVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGG+Y+FNLMQMCGV GVE 
Sbjct: 241 KENPHTVIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGEYRFNLMQMCGVEGVEV 300

Query: 351 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 389
           CPNPNEHISWDGVHLTQ TYKFMTHWLIHDIFPKLHCIV
Sbjct: 301 CPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 339

BLAST of Cucsat.G6733 vs. ExPASy TrEMBL
Match: A0A5A7TWR0 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001010 PE=3 SV=1)

HSP 1 Score: 676 bits (1744), Expect = 1.51e-244
Identity = 317/339 (93.51%), Postives = 327/339 (96.46%), Query Frame = 0

Query: 51  MFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGL 110
           MFDAIYQLGDSISDTGNLIREN NTPFSHLPYGQSFFNNPTGRCSNGLLMLDF ALDAGL
Sbjct: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 60

Query: 111 PLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNS 170
           PLVSPYLNKDG MDHGVNFAVAGSTALPSQYL ++YKI+SPVTNSSLDHQLDWMFSHFNS
Sbjct: 61  PLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNSSLDHQLDWMFSHFNS 120

Query: 171 ICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 230
           ICHNQR+CNEKLRSALFLVGEIGGNDYNYALFQGKTI+EAKDMVPDVVQTIKSAVEKVIS
Sbjct: 121 ICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVPDVVQTIKSAVEKVIS 180

Query: 231 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFATYHNDQIKQAIEVLK 290
           YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLN  ATY+N+QIKQ IEVLK
Sbjct: 181 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLATYYNNQIKQTIEVLK 240

Query: 291 KENPHAIIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEA 350
           KENPH +IVYGDYYNAFLWIIRHAFVLGFDE SLQKSCCGIGG+Y+FNLMQMCGV GVE 
Sbjct: 241 KENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEYRFNLMQMCGVEGVEV 300

Query: 351 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 389
           CPNPNEHISWDGVHLTQ TYKFMTHWLIHDIFPKLHCIV
Sbjct: 301 CPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 339

BLAST of Cucsat.G6733 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 2.3e-77
Identity = 159/361 (44.04%), Postives = 203/361 (56.23%), Query Frame = 0

Query: 27  SVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSHLPYGQSF 86
           S +  L LLV +L  S  H    C  ++IYQ GDSISDTGNLIR                
Sbjct: 2   STTKALSLLVFILFVSLVHSSDQCPINSIYQFGDSISDTGNLIR---------------- 61

Query: 87  FNNPTGRCSNGLLMLDFLALDAGLPLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSY 146
            N P                 A  P   P   ++   +  VNF V+GSTAL S + S   
Sbjct: 62  -NGP-----------------ASSPTPKPLPQRE--HNVFVNFGVSGSTALNSSFFSER- 121

Query: 147 KIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKT 206
            +  P TN+ L  QL W   H  S CH     ++ L+ +LF+VGEIGGNDYNY  FQGK 
Sbjct: 122 NLHVPATNTPLSMQLAWFKGHLRSTCHG--SSSDCLKHSLFMVGEIGGNDYNYGFFQGKP 181

Query: 207 IQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELH 266
           ++E +  +P VV  I +A  +VI  GA  VVVPGNFP+GCFPIYLT F   DT  YD+  
Sbjct: 182 MEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDYDDNG 241

Query: 267 CLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGFDEESLQK 326
           CL  LN FA  HN+Q+++AI  L+KE P   IVYGDYYNAF +++R      FD+    K
Sbjct: 242 CLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKSVALK 301

Query: 327 SCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLH 386
           SCCG GG Y ++  +  G  GV  C NP++ ISWDGVHLTQK Y+FM+ +L + I  ++ 
Sbjct: 302 SCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQILSQIK 320

Query: 387 C 388
           C
Sbjct: 362 C 320

BLAST of Cucsat.G6733 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.7e-76
Identity = 147/365 (40.27%), Postives = 218/365 (59.73%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLI---RENLNTP 79
           MA+     + LF  L V ++ S    P     F +I   GDSI+DTGNL+     N    
Sbjct: 1   MASLDSLVIFLFSTLFVTIVSSETPCP----NFKSIISFGDSIADTGNLVGLSDRNQLPV 60

Query: 80  FSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSPYL-NKDGLMDHGVNFAVAGST 139
            +  PYG++FF++PTGR  +G +++DF+A   GLP V PY  +K+   D GVNFAVAG+T
Sbjct: 61  TAFPPYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGAT 120

Query: 140 ALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGN 199
           AL S +L    + I P TN SL  QL        ++C +  +C + + +AL L+GEIGGN
Sbjct: 121 ALKSSFLKK--RGIQPHTNVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNALILMGEIGGN 180

Query: 200 DYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFH 259
           DYN+  F  K ++E +++VP V+ +I S + ++I  G    +VPG FPIGC  +YLT + 
Sbjct: 181 DYNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYK 240

Query: 260 TNDTSAYD-ELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHA 319
           T++   YD    CLK LN F  YH++++K  +  L+K  PH  I+Y DYYN+ L I +  
Sbjct: 241 TSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEP 300

Query: 320 FVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMT 379
              GF E     +CCGIGG Y FN  + CG  GV++C +P++++ WDGVH+T+  YK++ 
Sbjct: 301 AKFGFMERPF-PACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIA 358

BLAST of Cucsat.G6733 vs. TAIR 10
Match: AT1G31550.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.7e-75
Identity = 151/355 (42.54%), Postives = 219/355 (61.69%), Query Frame = 0

Query: 29  SLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNL--IRENLNTPFSHL-PYGQS 88
           SLFL  L + + SS++   +   F++I   GDSI+DTGNL  + ++ N P S   PYG++
Sbjct: 14  SLFLSTLFVSIVSSES---QCRNFESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGET 73

Query: 89  FFNNPTGRCSNGLLMLDFLALDAGLPLVSPYL-NKDGLMDHGVNFAVAGSTALPSQYLSS 148
           FF++PTGR S+G L++DF+A   GLP V PY  + +G  + GVNFAVA +TAL S +L  
Sbjct: 74  FFHHPTGRFSDGRLIIDFIAEFLGLPYVPPYFGSTNGNFEKGVNFAVASATALESSFLEE 133

Query: 149 SYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNYALFQG 208
             K      N SL  QL        ++C    +C + + +AL L+GEIG NDYN+  FQ 
Sbjct: 134 --KGYHCPHNFSLGVQLKIFKQSLPNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQL 193

Query: 209 KTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDE 268
           + + E K++VP V+ TI SA+ ++I  G    +VPG FP+GC   +LT   T++   YD 
Sbjct: 194 RPLDEVKELVPLVISTISSAITELIGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDP 253

Query: 269 L-HCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGFDEES 328
           L  CLK LN F  YH++Q+++ +  L+K NPH  I+Y DYYNA L + R     GF    
Sbjct: 254 LTGCLKWLNKFGEYHSEQLQEELNRLRKLNPHVNIIYADYYNASLRLGREPSKYGFINRH 313

Query: 329 LQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLI 379
           L  +CCG+GG Y FNL + CG  GVEAC +P+++++WDG+H+T+  +K M   L+
Sbjct: 314 L-SACCGVGGPYNFNLSRSCGSVGVEACSDPSKYVAWDGLHMTEAAHKSMADGLV 362

BLAST of Cucsat.G6733 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 278.1 bits (710), Expect = 1.1e-74
Identity = 149/364 (40.93%), Postives = 218/364 (59.89%), Query Frame = 0

Query: 20  MAASTKSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNL--IRENLNTP- 79
           MA      VS FL+L    L +  + P +   F +I   GDSI+DTGNL  + +  N P 
Sbjct: 1   MATLFMKLVSFFLILSTFCLTTVNSEP-QCHNFKSIISFGDSIADTGNLLALSDPTNLPK 60

Query: 80  FSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSP-YLNKDGLMDHGVNFAVAGST 139
            + LPYG++FF++PTGR SNG L++DF+A   G PLV P Y +++   + GVNFAV G+T
Sbjct: 61  VAFLPYGETFFHHPTGRFSNGRLIIDFIAEFLGFPLVPPFYGSQNANFEKGVNFAVGGAT 120

Query: 140 ALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGN 199
           AL   +L     I  P TN SL  QL        ++C +  +C + + ++L L+GEIGGN
Sbjct: 121 ALERSFLEER-GIHFPYTNVSLAVQLSSFKESLPNLCVSPSDCRDMIENSLILMGEIGGN 180

Query: 200 DYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFH 259
           DYNYA F GK I+E K++VP V++TI SA+ ++I  G    +VPG FP+GC   YL+ + 
Sbjct: 181 DYNYAFFVGKNIEEIKELVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQ 240

Query: 260 TNDTSAYDEL-HCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHA 319
           T++   YD L  CLK LN F+ YH++Q++  +  L+K  PH  I+Y DYYN  L + +  
Sbjct: 241 TSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEP 300

Query: 320 FVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMT 379
              GF    L  +CC +GG + F L +  G    E C +P++++SWDGVH+T+  Y+ M 
Sbjct: 301 AKFGFISRPL-PACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLMA 360

BLAST of Cucsat.G6733 vs. TAIR 10
Match: AT1G28580.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.4e-74
Identity = 149/360 (41.39%), Postives = 214/360 (59.44%), Query Frame = 0

Query: 25  KSSVSLFLLLLVLVLGSSKAHPLKACMFDAIYQLGDSISDTGNLIRENLNTPFSHL---P 84
           K  V +FL   V+   SS+    K   F +I   GDSI+DTGNL+  +      H+   P
Sbjct: 11  KLLVFIFLSTFVVTNVSSET---KCREFKSIISFGDSIADTGNLLGLSDPKDLPHMAFPP 70

Query: 85  YGQSFFNNPTGRCSNGLLMLDFLALDAGLPLVSP-YLNKDGLMDHGVNFAVAGSTALPSQ 144
           YG++FF++PTGR SNG L++DF+A   GLPLV P Y + +   + GVNFAV G+TAL   
Sbjct: 71  YGENFFHHPTGRFSNGRLIIDFIAEFLGLPLVPPFYGSHNANFEKGVNFAVGGATALERS 130

Query: 145 YLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKLRSALFLVGEIGGNDYNYA 204
           +L     I  P TN SL  QL+       SIC +  +C + + +AL L+GEIGGNDYNYA
Sbjct: 131 FLEDR-GIHFPYTNVSLGVQLNSFKESLPSICGSPSDCRDMIENALILMGEIGGNDYNYA 190

Query: 205 LFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNFPIGCFPIYLTGFHTNDTS 264
            F  K I+E K+++P V+ TI SA+ ++I  G    +VPG FP+GC  +YLT   T++  
Sbjct: 191 FFVDKGIEEIKELMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSHQTSNME 250

Query: 265 AYDEL-HCLKDLNSFATYHNDQIKQAIEVLKKENPHAIIVYGDYYNAFLWIIRHAFVLGF 324
            YD L  CLK LN F   H +Q++  +  L+K  PH  I+Y DYYNA   + +     GF
Sbjct: 251 EYDPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQEPAKFGF 310

Query: 325 DEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDGVHLTQKTYKFMTHWLIH 380
               L  +CCG GG Y + + + CG   VE+C +P+++++WDGVH+T+  Y+ M   +++
Sbjct: 311 MNRPL-SACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLMAEGILN 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3MKY26.2e-8344.28Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1[more]
Q9LZB23.3e-7644.04GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2... [more]
Q94F408.1e-7540.27GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2... [more]
Q9C8572.4e-7442.54GDSL esterase/lipase At1g31550 OS=Arabidopsis thaliana OX=3702 GN=At1g31550 PE=2... [more]
Q9FXJ11.5e-7340.93GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3... [more]
Match NameE-valueIdentityDescription
XP_011659746.14.20e-27798.92acetylajmalan esterase-like [Cucumis sativus][more]
XP_016903430.13.96e-26694.59PREDICTED: acetylajmalan esterase-like [Cucumis melo][more]
TYK13517.12.29e-26594.32acetylajmalan esterase-like [Cucumis melo var. makuwa][more]
KAA0032295.16.57e-26594.32acetylajmalan esterase-like [Cucumis melo var. makuwa][more]
XP_031737213.13.10e-26192.16acetylajmalan esterase-like [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A1S4E6291.92e-26694.59acetylajmalan esterase-like OS=Cucumis melo OX=3656 GN=LOC103503259 PE=3 SV=1[more]
A0A5D3CNR61.11e-26594.32Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A5A7SSW23.18e-26594.32Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A5A7VJA52.62e-24593.81Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A5A7TWR01.51e-24493.51Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
Match NameE-valueIdentityDescription
AT5G03980.12.3e-7744.04SGNH hydrolase-type esterase superfamily protein [more]
AT1G28600.15.7e-7640.27GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G31550.21.7e-7542.54GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G28570.11.1e-7440.93SGNH hydrolase-type esterase superfamily protein [more]
AT1G28580.11.4e-7441.39GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 55..377
e-value: 2.0E-33
score: 116.7
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 42..381
e-value: 1.4E-67
score: 230.4
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 35..387
NoneNo IPR availablePANTHERPTHR22835:SF581ACETYLAJMALAN ESTERASE-LIKEcoord: 35..387
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 55..379
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 54..381
e-value: 7.72233E-109
score: 320.331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G6733.T1Cucsat.G6733.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds