Cucsat.G6431 (gene) Cucumber (B10) v3

BLAST of Cucsat.G6431 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.0e-39
Identity = 113/337 (33.53%), Postives = 172/337 (51.04%), Query Frame = 0

Query: 11  LTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYF--NQAFLITCDKAFNPPK 70
           +  +  L+       Q + +C   CG++ + YPFG   GCY+  +++F +TC++     K
Sbjct: 9   VVAVFYLAYTQLVKGQPRKECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNE---QEK 68

Query: 71  AFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPATLPIADGK 130
            F    N+ V N+SL+G+L +     R CY+       T +I   T L       +    
Sbjct: 69  LFF--GNMPVINMSLSGQLRVRLVRSRVCYDSQG--KQTDYIAQRTTLGNFTLSEL---- 128

Query: 131 NKFIAIGCNTFGLF--TGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDIPNGLSD 190
           N+F  +GCN++     +G+ K   + TGC++IC +++   +GSCSG GCC++ +P G S 
Sbjct: 129 NRFTVVGCNSYAFLRTSGVEK---YSTGCISIC-DSATTKNGSCSGEGCCQIPVPRGYS- 188

Query: 191 LSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKS-SFIDNFED-KEVEVVVDWSIGN 250
             + V P     + ++   N C YAF+V +  F F +   ++N  +     VV+DWSIG+
Sbjct: 189 -FVRVKPHSFHNHPTVHLFNPCTYAFLVEDGMFDFHALEDLNNLRNVTTFPVVLDWSIGD 248

Query: 251 ETIID-----VCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELN 310
           +T        VCG NS      S   + Y C+C +G+EGNPYLP GC QDINEC     N
Sbjct: 249 KTCKQVEYRGVCGGNS--TCFDSTGGTGYNCKCLEGFEGNPYLPNGC-QDINECISSRHN 308

Query: 311 DCTHE-CINTNGSYTCKCPKNYKGDGRRGEDGHGCTR 336
              H  C NT GS+ C CP  Y+ D       + CTR
Sbjct: 309 CSEHSTCENTKGSFNCNCPSGYRKDSL-----NSCTR 320

BLAST of Cucsat.G6431 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 4.0e-36
Identity = 102/318 (32.08%), Postives = 155/318 (48.74%), Query Frame = 0

Query: 11  LTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYF--NQAFLITCDKAFNPPK 70
           + +   L+      +Q + DC   CGD+ I YPFG+  GCY+  + +F ITC++  + P 
Sbjct: 9   MAIFFYLAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE--DKPN 68

Query: 71  AFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPATLPIADGK 130
                +NI V N + +G+L  L P    CY+               NLS           
Sbjct: 69  VL---SNIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLS-------FSPN 128

Query: 131 NKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDIPNGLSDLS 190
           NKF  +GCN + L +       + TGC+++C +     +  C+G GCC  ++   L    
Sbjct: 129 NKFTLVGCNAWALLS-TFGIQNYSTGCMSLC-DTPPPPNSKCNGVGCCRTEVSIPLDSHR 188

Query: 191 LAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFED----KEVEVVVDWSIGN 250
           +   P   +   S+   N C YAF V +  F F S  +++ +D        V++DWSIGN
Sbjct: 189 IETQPSRFENMTSVEHFNPCSYAFFVEDGMFNFSS--LEDLKDLRNVTRFPVLLDWSIGN 248

Query: 251 ETIIDVCGIN--SKRNSSFSDDRSQ-YRCQCPDGYEGNPYLPQGCDQDINECEHKELNDC 310
           +T   V G N     ++ F   R + Y C+C  G++GNPYL  GC QDINEC  + +++C
Sbjct: 249 QTCEQVVGRNICGGNSTCFDSTRGKGYNCKCLQGFDGNPYLSDGC-QDINECTTR-IHNC 308

Query: 311 --THECINTNGSYTCKCP 318
             T  C NT GS+ C+CP
Sbjct: 309 SDTSTCENTLGSFHCQCP 308

BLAST of Cucsat.G6431 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 1.7e-34
Identity = 120/377 (31.83%), Postives = 181/377 (48.01%), Query Frame = 0

Query: 9   VGLTVIILL--STLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYF--NQAFLITCDKAF 68
           V L VI  L  + L     Q + DC   CG++ I YPFG+  GCY+  +  F +TC    
Sbjct: 7   VFLVVIFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC---- 66

Query: 69  NPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPF---IPNTTNLSAPAT 128
              +  L    I VTNIS +G + +L      CYE     +GT     + ++ +LS+   
Sbjct: 67  VVEEKLLLFGIIQVTNISHSGHVSVLFERFSECYEQKNETNGTALGYQLGSSFSLSS--- 126

Query: 129 LPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCC---EL 188
                  NKF  +GCN   L +   K   + TGC+++C N+    +G C+G GCC   + 
Sbjct: 127 ------NNKFTLVGCNALSLLSTFGK-QNYSTGCLSLC-NSQPEANGRCNGVGCCTTEDF 186

Query: 189 DIP-------NGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSS-FIDNFE 248
            +P        G   L   V   L   N S+ + N C YAF+V +  F F SS  + N  
Sbjct: 187 SVPFDSDTFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLR 246

Query: 249 D-KEVEVVVDWSIGNETI-----IDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQ 308
           +     V +DWSIGN+T        +CG NS   +S +  R+ Y C+C +GY+GNPY  +
Sbjct: 247 NVTRFPVALDWSIGNQTCEQAGSTRICGKNSSCYNSTT--RNGYICKCNEGYDGNPYRSE 306

Query: 309 GCDQDINECEHKELNDCT--HECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKAIPI 360
           GC +DI+EC   + ++C+    C N +G + CKCP  Y  +           R    + I
Sbjct: 307 GC-KDIDEC-ISDTHNCSDPKTCRNRDGGFDCKCPSGYDLNSSMSCTRPEYKRTRIFLVI 361

BLAST of Cucsat.G6431 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 144.4 bits (363), Expect = 2.4e-33
Identity = 101/332 (30.42%), Postives = 154/332 (46.39%), Query Frame = 0

Query: 8   LVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYF--NQAFLITCDKAFN 67
           LV +   +  + L     Q   +C   CG++ I YPFG+  GCY+  N++F ITC +  +
Sbjct: 9   LVAIFFSLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE--D 68

Query: 68  PPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPATLPIA 127
            P      ++I V N + +G+L +L      CY++    +         NLS  A     
Sbjct: 69  RPHVL---SDIEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLSA----- 128

Query: 128 DGKNKFIAIGCNTFGLFT--GMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDIPNG 187
              NK  A+GCN   L    GM     + T C+++C ++    DG C+G GCC +D+   
Sbjct: 129 --NNKLTAVGCNALSLLDTFGM---QNYSTACLSLC-DSPPEADGECNGRGCCRVDVSAP 188

Query: 188 LSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSS--FIDNFEDKEVEVVVDWS 247
           L   +            S    + C YAF+V ++ F F S+   ++        V++DWS
Sbjct: 189 LDSYTFETTSGRIKHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWS 248

Query: 248 IGNETI-----IDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECE-- 307
           +GN+T        +CG NS      S  R+ Y C+C +G++GNPYL  GC QD+NEC   
Sbjct: 249 VGNQTCEQVGSTSICGGNS--TCLDSTPRNGYICRCNEGFDGNPYLSAGC-QDVNECTTS 308

Query: 308 ---HKELNDCTHECINTNGSYTCKCPKNYKGD 324
              H+        C N  G + CKC   Y+ D
Sbjct: 309 STIHRHNCSDPKTCRNKVGGFYCKCQSGYRLD 321

BLAST of Cucsat.G6431 vs. ExPASy Swiss-Prot
Match: Q9SA25 (Wall-associated receptor kinase-like 8 OS=Arabidopsis thaliana OX=3702 GN=WAKL8 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 6.4e-26
Identity = 101/322 (31.37%), Postives = 145/322 (45.03%), Query Frame = 0

Query: 11  LTVIILLSTLASAASQAKP----DCDEWCGDLRIPYPFGVKQGCYFNQAFLITCDKAFN- 70
           L V++LL     AA+   P    +C + CG++ +PYPFG+ +GCY N+ F I C  + + 
Sbjct: 9   LVVMLLLRICEYAAASTFPLALRNCSDHCGNVSVPYPFGIGKGCYKNKWFEIVCKSSSDQ 68

Query: 71  PPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIP--NTTNLSAPATLP 130
            P   L     +VT+ +L G+   +    ++  +     SG P     ++++L+   +  
Sbjct: 69  QPILLLPRIRRAVTSFNL-GDPFSISVYNKFYIQSPLKHSGCPNRDGYSSSSLNLKGSPF 128

Query: 131 IADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDG---SCSGTGCCELDI 190
                NKF A+GCN       M   G  + GC   C N      G   SC G  CC++ I
Sbjct: 129 FISENNKFTAVGCNNKAF---MNVTGLQIVGCETTCGNEIRSYKGANTSCVGYKCCQMTI 188

Query: 191 PNGLSDLSLAVGPVLPDTNRSLVKN-NSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVD 250
           P       L    V   T   L  N   C  AF+     F    S     E  E      
Sbjct: 189 P------PLLQLQVFDATVEKLEPNKQGCQVAFLT---QFTLSGSLFTPPELMEYSEYT- 248

Query: 251 WSIGNETIIDVCGINSKR-----NSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECE 310
            +I  E  +D+  + SKR     N+ F D    Y+C C +GYEGNPY+P GC QDI+EC 
Sbjct: 249 -TIELEWRLDLSYMTSKRVLCKGNTFFED---SYQCSCHNGYEGNPYIPGGC-QDIDECR 308

Query: 311 HKELNDC-THECINTNGSYTCK 316
              LN C   +C+N  GSY C+
Sbjct: 309 DPHLNKCGKRKCVNVLGSYRCE 311

BLAST of Cucsat.G6431 vs. NCBI nr
Match: XP_031743922.1 (wall-associated receptor kinase 5-like [Cucumis sativus] >KAE8647202.1 hypothetical protein Csa_018972 [Cucumis sativus])

HSP 1 Score: 727 bits (1876), Expect = 2.20e-258
Identity = 345/346 (99.71%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60
           MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC
Sbjct: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60

Query: 61  DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA 120
           DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA
Sbjct: 61  DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA 120

Query: 121 TLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDI 180
           TLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDI
Sbjct: 121 TLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDI 180

Query: 181 PNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVDW 240
           PNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVDW
Sbjct: 181 PNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVDW 240

Query: 241 SIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELND 300
           SIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELND
Sbjct: 241 SIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELND 300

Query: 301 CTHECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKAIPIIIGM 346
           CTHECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKAIPIIIG+
Sbjct: 301 CTHECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKAIPIIIGI 346

BLAST of Cucsat.G6431 vs. NCBI nr
Match: XP_008441599.1 (PREDICTED: LOW QUALITY PROTEIN: wall-associated receptor kinase 2-like [Cucumis melo] >TYK18667.1 wall-associated receptor kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 615 bits (1587), Expect = 1.43e-214
Identity = 294/346 (84.97%), Postives = 310/346 (89.60%), Query Frame = 0

Query: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60
           MERLRKTLVGLTVII+LST ASA S AKP CDEWCGDLRIPYP+GVK+GCY NQ FLITC
Sbjct: 1   MERLRKTLVGLTVIIILSTPASAVSLAKPGCDEWCGDLRIPYPYGVKEGCYLNQTFLITC 60

Query: 61  DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA 120
           DKAF+PPKAFL DTNISVTNISLNGELHMLQPIVR C+E VQL  G+ FIPN TNL+AP 
Sbjct: 61  DKAFSPPKAFLMDTNISVTNISLNGELHMLQPIVRDCHEGVQLGGGS-FIPNITNLTAPP 120

Query: 121 TLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDI 180
           TL IADGKNKFIAIGCNTFGLFTGMLKG EFLTGCVA+CTNNS IVDGSCSGTGCCEL+I
Sbjct: 121 TLSIADGKNKFIAIGCNTFGLFTGMLKGSEFLTGCVAVCTNNSSIVDGSCSGTGCCELNI 180

Query: 181 PNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVDW 240
           PNGL  L LAVG +L D N + VK N CGYAFVVGEEGFKFKSS+IDNFEDKEV  VVDW
Sbjct: 181 PNGLRSLGLAVGQLLDDRNGTFVKYNPCGYAFVVGEEGFKFKSSYIDNFEDKEVVAVVDW 240

Query: 241 SIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELND 300
           SIGNETIID+CG+NS RNSSFSDDRSQYRC+C DGYEGNPYLPQGCDQDINECEHK LND
Sbjct: 241 SIGNETIIDICGLNSIRNSSFSDDRSQYRCECLDGYEGNPYLPQGCDQDINECEHKWLND 300

Query: 301 CTHECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKAIPIIIGM 346
           CTHECINT GSYTCKCPKNYKGDGRRGED  GCTRDSK IPIIIG+
Sbjct: 301 CTHECINTRGSYTCKCPKNYKGDGRRGEDRQGCTRDSKTIPIIIGI 345

BLAST of Cucsat.G6431 vs. NCBI nr
Match: XP_031743923.1 (wall-associated receptor kinase 2 [Cucumis sativus] >KAE8647200.1 hypothetical protein Csa_018982 [Cucumis sativus])

HSP 1 Score: 614 bits (1583), Expect = 5.96e-214
Identity = 295/347 (85.01%), Postives = 316/347 (91.07%), Query Frame = 0

Query: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60
           MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC
Sbjct: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60

Query: 61  DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA 120
           DKAFNPPKAFLKDTNISVTNISLNGELH+LQPIVR+C EDV LV+ + FIPNTTNL A A
Sbjct: 61  DKAFNPPKAFLKDTNISVTNISLNGELHILQPIVRFCNEDVSLVNRS-FIPNTTNLPATA 120

Query: 121 TLPIADGKNKFIAIGCNTFGLFTGMLKGGE-FLTGCVAICTNNSIIVDGSCSGTGCCELD 180
           T PIADGKNKFIAIGCNTFG FTG LKGG+ FLTGC+A+C NN+     SCSG GCC+LD
Sbjct: 121 TFPIADGKNKFIAIGCNTFGFFTGKLKGGDQFLTGCIAVCPNNNKNNTWSCSGNGCCKLD 180

Query: 181 IPNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVD 240
           IP+G SDL+L V P L DT+R+LV+N  CGYAFVVGEEGF+FK S+IDNFED EVEVVVD
Sbjct: 181 IPDGSSDLNLTVAPALLDTDRNLVQNKPCGYAFVVGEEGFEFKQSYIDNFEDTEVEVVVD 240

Query: 241 WSIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELN 300
           WS  +E IIDVC  ++KRNS+FSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELN
Sbjct: 241 WSTESE-IIDVCRKDTKRNSNFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELN 300

Query: 301 DCTHECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKAIPIIIGM 346
           DCTHECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKAIPIIIG+
Sbjct: 301 DCTHECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKAIPIIIGI 345

BLAST of Cucsat.G6431 vs. NCBI nr
Match: XP_038886589.1 (putative wall-associated receptor kinase-like 16 [Benincasa hispida])

HSP 1 Score: 501 bits (1290), Expect = 1.07e-169
Identity = 246/350 (70.29%), Postives = 291/350 (83.14%), Query Frame = 0

Query: 1   MERLRKTLVGLTVII---LLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFL 60
           M+R RKTLVGL +II   +LST   A+SQA   CDEWCGDLRIPYPFGVKQGC+ NQ FL
Sbjct: 1   MKRWRKTLVGLMLIIKIAILSTAVVASSQALAGCDEWCGDLRIPYPFGVKQGCHLNQTFL 60

Query: 61  ITCDKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLS 120
           ITC+K  +PPKAFL DT+ISVTNISL+GELH+LQPIVRYCYE VQ+    PFIPN TNLS
Sbjct: 61  ITCNKTNSPPKAFLMDTDISVTNISLHGELHILQPIVRYCYEQVQV--SDPFIPNATNLS 120

Query: 121 APATLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCE 180
            PA LPIADGKNKFIA GCNTFGLF+GMLKG EFL+GC+++CTN S IVDGSC G GCCE
Sbjct: 121 VPAALPIADGKNKFIATGCNTFGLFSGMLKGSEFLSGCISVCTNGSAIVDGSCFGNGCCE 180

Query: 181 LDIPNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVV 240
           L+IP GL++LSL VG +LP+   +++K N CGYAFVVG+E FKF S++I  FED+EVEVV
Sbjct: 181 LEIPKGLTNLSLFVGQLLPN-ETNILKYNPCGYAFVVGDERFKFDSTYIQRFEDEEVEVV 240

Query: 241 VDWSIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKE 300
           VDW+IGN+T+ +VC  NS+R S+FSDD SQYRC+C DG+ GNPYLPQGC +DI+EC+ ++
Sbjct: 241 VDWAIGNQTM-NVCRQNSQRISNFSDDGSQYRCECLDGFMGNPYLPQGC-EDIDECKDEK 300

Query: 301 LNDCTHECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKA-IPIIIGM 346
           LN C +ECINT G+YTCKCPKN+KGDGR G  G GCTRDSKA IPIIIG+
Sbjct: 301 LNKCKYECINTIGNYTCKCPKNFKGDGRHG--GEGCTRDSKAFIPIIIGI 343

BLAST of Cucsat.G6431 vs. NCBI nr
Match: XP_008441597.1 (PREDICTED: wall-associated receptor kinase 2-like [Cucumis melo] >TYK18640.1 wall-associated receptor kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 469 bits (1208), Expect = 2.32e-157
Identity = 227/349 (65.04%), Postives = 280/349 (80.23%), Query Frame = 0

Query: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60
           M R   T+V +  IIL + +  A+SQA PDCDEWCGDL+IPYPFG+KQGCY +Q+FLITC
Sbjct: 1   MGRWTNTVVRVVTIILSTAIVVASSQALPDCDEWCGDLQIPYPFGLKQGCYLSQSFLITC 60

Query: 61  DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA 120
           +K  +PP AFL DTNISVT ISLNGELHMLQPIVRYCY +V  V G PF+PN+TNLS P 
Sbjct: 61  NKTTSPPMAFLMDTNISVTKISLNGELHMLQPIVRYCYAEVP-VDG-PFVPNSTNLSVPT 120

Query: 121 TLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDI 180
           TLPIADGKNKF+AIGCNTFGLF G+L G EFLTGC+++C  +S   DG C+G GCCEL+I
Sbjct: 121 TLPIADGKNKFVAIGCNTFGLFGGILNGSEFLTGCISLCLKDSSTDDGVCNGNGCCELEI 180

Query: 181 PNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVDW 240
           PNGL DLSL VG +LPD   + +K N CG+AFVVG+EGF+F+S + ++F+D EVEVV  W
Sbjct: 181 PNGLMDLSLFVGQLLPDL--TFIKYNPCGHAFVVGDEGFEFESKYTESFKDVEVEVVTSW 240

Query: 241 SIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELND 300
           +IGNET  D CG +S+RNSSFS+D S++ CQC +G++GNPYLPQGC QDI+EC+ ++LN 
Sbjct: 241 AIGNETNFD-CGSHSERNSSFSNDGSKFHCQCFEGFQGNPYLPQGC-QDIDECKDEKLNQ 300

Query: 301 CTHE--CINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKA-IPIIIGM 346
           C ++  C+NT G+YTCKCPKN+KGDGR G  G GCTRD KA +PIIIG+
Sbjct: 301 CKYKSKCVNTIGNYTCKCPKNFKGDGRHG--GEGCTRDYKAFLPIIIGI 341

BLAST of Cucsat.G6431 vs. ExPASy TrEMBL
Match: A0A0A0KCX3 (GUB_WAK_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G354370 PE=4 SV=1)

HSP 1 Score: 602 bits (1551), Expect = 5.19e-216
Identity = 288/290 (99.31%), Postives = 290/290 (100.00%), Query Frame = 0

Query: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60
           MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC
Sbjct: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60

Query: 61  DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA 120
           DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA
Sbjct: 61  DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA 120

Query: 121 TLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDI 180
           TLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDI
Sbjct: 121 TLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDI 180

Query: 181 PNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVDW 240
           PNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVDW
Sbjct: 181 PNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVDW 240

Query: 241 SIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDI 290
           SIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQ++
Sbjct: 241 SIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQEL 290

BLAST of Cucsat.G6431 vs. ExPASy TrEMBL
Match: A0A5D3D534 (Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold481G00020 PE=4 SV=1)

HSP 1 Score: 615 bits (1587), Expect = 6.92e-215
Identity = 294/346 (84.97%), Postives = 310/346 (89.60%), Query Frame = 0

Query: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60
           MERLRKTLVGLTVII+LST ASA S AKP CDEWCGDLRIPYP+GVK+GCY NQ FLITC
Sbjct: 1   MERLRKTLVGLTVIIILSTPASAVSLAKPGCDEWCGDLRIPYPYGVKEGCYLNQTFLITC 60

Query: 61  DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA 120
           DKAF+PPKAFL DTNISVTNISLNGELHMLQPIVR C+E VQL  G+ FIPN TNL+AP 
Sbjct: 61  DKAFSPPKAFLMDTNISVTNISLNGELHMLQPIVRDCHEGVQLGGGS-FIPNITNLTAPP 120

Query: 121 TLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDI 180
           TL IADGKNKFIAIGCNTFGLFTGMLKG EFLTGCVA+CTNNS IVDGSCSGTGCCEL+I
Sbjct: 121 TLSIADGKNKFIAIGCNTFGLFTGMLKGSEFLTGCVAVCTNNSSIVDGSCSGTGCCELNI 180

Query: 181 PNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVDW 240
           PNGL  L LAVG +L D N + VK N CGYAFVVGEEGFKFKSS+IDNFEDKEV  VVDW
Sbjct: 181 PNGLRSLGLAVGQLLDDRNGTFVKYNPCGYAFVVGEEGFKFKSSYIDNFEDKEVVAVVDW 240

Query: 241 SIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELND 300
           SIGNETIID+CG+NS RNSSFSDDRSQYRC+C DGYEGNPYLPQGCDQDINECEHK LND
Sbjct: 241 SIGNETIIDICGLNSIRNSSFSDDRSQYRCECLDGYEGNPYLPQGCDQDINECEHKWLND 300

Query: 301 CTHECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKAIPIIIGM 346
           CTHECINT GSYTCKCPKNYKGDGRRGED  GCTRDSK IPIIIG+
Sbjct: 301 CTHECINTRGSYTCKCPKNYKGDGRRGEDRQGCTRDSKTIPIIIGI 345

BLAST of Cucsat.G6431 vs. ExPASy TrEMBL
Match: A0A1S3B4F2 (LOW QUALITY PROTEIN: wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485679 PE=4 SV=1)

HSP 1 Score: 615 bits (1587), Expect = 6.92e-215
Identity = 294/346 (84.97%), Postives = 310/346 (89.60%), Query Frame = 0

Query: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60
           MERLRKTLVGLTVII+LST ASA S AKP CDEWCGDLRIPYP+GVK+GCY NQ FLITC
Sbjct: 1   MERLRKTLVGLTVIIILSTPASAVSLAKPGCDEWCGDLRIPYPYGVKEGCYLNQTFLITC 60

Query: 61  DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA 120
           DKAF+PPKAFL DTNISVTNISLNGELHMLQPIVR C+E VQL  G+ FIPN TNL+AP 
Sbjct: 61  DKAFSPPKAFLMDTNISVTNISLNGELHMLQPIVRDCHEGVQLGGGS-FIPNITNLTAPP 120

Query: 121 TLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDI 180
           TL IADGKNKFIAIGCNTFGLFTGMLKG EFLTGCVA+CTNNS IVDGSCSGTGCCEL+I
Sbjct: 121 TLSIADGKNKFIAIGCNTFGLFTGMLKGSEFLTGCVAVCTNNSSIVDGSCSGTGCCELNI 180

Query: 181 PNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVDW 240
           PNGL  L LAVG +L D N + VK N CGYAFVVGEEGFKFKSS+IDNFEDKEV  VVDW
Sbjct: 181 PNGLRSLGLAVGQLLDDRNGTFVKYNPCGYAFVVGEEGFKFKSSYIDNFEDKEVVAVVDW 240

Query: 241 SIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELND 300
           SIGNETIID+CG+NS RNSSFSDDRSQYRC+C DGYEGNPYLPQGCDQDINECEHK LND
Sbjct: 241 SIGNETIIDICGLNSIRNSSFSDDRSQYRCECLDGYEGNPYLPQGCDQDINECEHKWLND 300

Query: 301 CTHECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKAIPIIIGM 346
           CTHECINT GSYTCKCPKNYKGDGRRGED  GCTRDSK IPIIIG+
Sbjct: 301 CTHECINTRGSYTCKCPKNYKGDGRRGEDRQGCTRDSKTIPIIIGI 345

BLAST of Cucsat.G6431 vs. ExPASy TrEMBL
Match: A0A0A0KFE8 (GUB_WAK_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G352870 PE=4 SV=1)

HSP 1 Score: 489 bits (1259), Expect = 9.08e-172
Identity = 239/292 (81.85%), Postives = 260/292 (89.04%), Query Frame = 0

Query: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60
           MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC
Sbjct: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60

Query: 61  DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA 120
           DKAFNPPKAFLKDTNISVTNISLNGELH+LQPIVR+C EDV LV+ + FIPNTTNL A A
Sbjct: 61  DKAFNPPKAFLKDTNISVTNISLNGELHILQPIVRFCNEDVSLVNRS-FIPNTTNLPATA 120

Query: 121 TLPIADGKNKFIAIGCNTFGLFTGMLKGGE-FLTGCVAICTNNSIIVDGSCSGTGCCELD 180
           T PIADGKNKFIAIGCNTFG FTG LKGG+ FLTGC+A+C NN+     SCSG GCC+LD
Sbjct: 121 TFPIADGKNKFIAIGCNTFGFFTGKLKGGDQFLTGCIAVCPNNNKNNTWSCSGNGCCKLD 180

Query: 181 IPNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVD 240
           IP+G SDL+L V P L DT+R+LV+N  CGYAFVVGEEGF+FK S+IDNFED EVEVVVD
Sbjct: 181 IPDGSSDLNLTVAPALLDTDRNLVQNKPCGYAFVVGEEGFEFKQSYIDNFEDTEVEVVVD 240

Query: 241 WSIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDIN 291
           WS  +E IIDVC  ++KRNS+FSDDRSQYRCQCPDGYEGNPYLPQGCDQ +N
Sbjct: 241 WSTESE-IIDVCRKDTKRNSNFSDDRSQYRCQCPDGYEGNPYLPQGCDQGMN 290

BLAST of Cucsat.G6431 vs. ExPASy TrEMBL
Match: A0A5D3D554 (Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold390G00010 PE=4 SV=1)

HSP 1 Score: 469 bits (1208), Expect = 1.12e-157
Identity = 227/349 (65.04%), Postives = 280/349 (80.23%), Query Frame = 0

Query: 1   MERLRKTLVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYFNQAFLITC 60
           M R   T+V +  IIL + +  A+SQA PDCDEWCGDL+IPYPFG+KQGCY +Q+FLITC
Sbjct: 1   MGRWTNTVVRVVTIILSTAIVVASSQALPDCDEWCGDLQIPYPFGLKQGCYLSQSFLITC 60

Query: 61  DKAFNPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPA 120
           +K  +PP AFL DTNISVT ISLNGELHMLQPIVRYCY +V  V G PF+PN+TNLS P 
Sbjct: 61  NKTTSPPMAFLMDTNISVTKISLNGELHMLQPIVRYCYAEVP-VDG-PFVPNSTNLSVPT 120

Query: 121 TLPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDI 180
           TLPIADGKNKF+AIGCNTFGLF G+L G EFLTGC+++C  +S   DG C+G GCCEL+I
Sbjct: 121 TLPIADGKNKFVAIGCNTFGLFGGILNGSEFLTGCISLCLKDSSTDDGVCNGNGCCELEI 180

Query: 181 PNGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVDW 240
           PNGL DLSL VG +LPD   + +K N CG+AFVVG+EGF+F+S + ++F+D EVEVV  W
Sbjct: 181 PNGLMDLSLFVGQLLPDL--TFIKYNPCGHAFVVGDEGFEFESKYTESFKDVEVEVVTSW 240

Query: 241 SIGNETIIDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELND 300
           +IGNET  D CG +S+RNSSFS+D S++ CQC +G++GNPYLPQGC QDI+EC+ ++LN 
Sbjct: 241 AIGNETNFD-CGSHSERNSSFSNDGSKFHCQCFEGFQGNPYLPQGC-QDIDECKDEKLNQ 300

Query: 301 CTHE--CINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKA-IPIIIGM 346
           C ++  C+NT G+YTCKCPKN+KGDGR G  G GCTRD KA +PIIIG+
Sbjct: 301 CKYKSKCVNTIGNYTCKCPKNFKGDGRHG--GEGCTRDYKAFLPIIIGI 341

BLAST of Cucsat.G6431 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 165.6 bits (418), Expect = 7.2e-41
Identity = 113/337 (33.53%), Postives = 172/337 (51.04%), Query Frame = 0

Query: 11  LTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYF--NQAFLITCDKAFNPPK 70
           +  +  L+       Q + +C   CG++ + YPFG   GCY+  +++F +TC++     K
Sbjct: 9   VVAVFYLAYTQLVKGQPRKECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNE---QEK 68

Query: 71  AFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPATLPIADGK 130
            F    N+ V N+SL+G+L +     R CY+       T +I   T L       +    
Sbjct: 69  LFF--GNMPVINMSLSGQLRVRLVRSRVCYDSQG--KQTDYIAQRTTLGNFTLSEL---- 128

Query: 131 NKFIAIGCNTFGLF--TGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDIPNGLSD 190
           N+F  +GCN++     +G+ K   + TGC++IC +++   +GSCSG GCC++ +P G S 
Sbjct: 129 NRFTVVGCNSYAFLRTSGVEK---YSTGCISIC-DSATTKNGSCSGEGCCQIPVPRGYS- 188

Query: 191 LSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKS-SFIDNFED-KEVEVVVDWSIGN 250
             + V P     + ++   N C YAF+V +  F F +   ++N  +     VV+DWSIG+
Sbjct: 189 -FVRVKPHSFHNHPTVHLFNPCTYAFLVEDGMFDFHALEDLNNLRNVTTFPVVLDWSIGD 248

Query: 251 ETIID-----VCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECEHKELN 310
           +T        VCG NS      S   + Y C+C +G+EGNPYLP GC QDINEC     N
Sbjct: 249 KTCKQVEYRGVCGGNS--TCFDSTGGTGYNCKCLEGFEGNPYLPNGC-QDINECISSRHN 308

Query: 311 DCTHE-CINTNGSYTCKCPKNYKGDGRRGEDGHGCTR 336
              H  C NT GS+ C CP  Y+ D       + CTR
Sbjct: 309 CSEHSTCENTKGSFNCNCPSGYRKDSL-----NSCTR 320

BLAST of Cucsat.G6431 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 153.7 bits (387), Expect = 2.8e-37
Identity = 102/318 (32.08%), Postives = 155/318 (48.74%), Query Frame = 0

Query: 11  LTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYF--NQAFLITCDKAFNPPK 70
           + +   L+      +Q + DC   CGD+ I YPFG+  GCY+  + +F ITC++  + P 
Sbjct: 9   MAIFFYLAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE--DKPN 68

Query: 71  AFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPATLPIADGK 130
                +NI V N + +G+L  L P    CY+               NLS           
Sbjct: 69  VL---SNIEVLNFNHSGQLRGLIPRSTVCYDQQTNNDFESLWFRLDNLS-------FSPN 128

Query: 131 NKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDIPNGLSDLS 190
           NKF  +GCN + L +       + TGC+++C +     +  C+G GCC  ++   L    
Sbjct: 129 NKFTLVGCNAWALLS-TFGIQNYSTGCMSLC-DTPPPPNSKCNGVGCCRTEVSIPLDSHR 188

Query: 191 LAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSSFIDNFED----KEVEVVVDWSIGN 250
           +   P   +   S+   N C YAF V +  F F S  +++ +D        V++DWSIGN
Sbjct: 189 IETQPSRFENMTSVEHFNPCSYAFFVEDGMFNFSS--LEDLKDLRNVTRFPVLLDWSIGN 248

Query: 251 ETIIDVCGIN--SKRNSSFSDDRSQ-YRCQCPDGYEGNPYLPQGCDQDINECEHKELNDC 310
           +T   V G N     ++ F   R + Y C+C  G++GNPYL  GC QDINEC  + +++C
Sbjct: 249 QTCEQVVGRNICGGNSTCFDSTRGKGYNCKCLQGFDGNPYLSDGC-QDINECTTR-IHNC 308

Query: 311 --THECINTNGSYTCKCP 318
             T  C NT GS+ C+CP
Sbjct: 309 SDTSTCENTLGSFHCQCP 308

BLAST of Cucsat.G6431 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 148.3 bits (373), Expect = 1.2e-35
Identity = 120/377 (31.83%), Postives = 181/377 (48.01%), Query Frame = 0

Query: 9   VGLTVIILL--STLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYF--NQAFLITCDKAF 68
           V L VI  L  + L     Q + DC   CG++ I YPFG+  GCY+  +  F +TC    
Sbjct: 7   VFLVVIFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTC---- 66

Query: 69  NPPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPF---IPNTTNLSAPAT 128
              +  L    I VTNIS +G + +L      CYE     +GT     + ++ +LS+   
Sbjct: 67  VVEEKLLLFGIIQVTNISHSGHVSVLFERFSECYEQKNETNGTALGYQLGSSFSLSS--- 126

Query: 129 LPIADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCC---EL 188
                  NKF  +GCN   L +   K   + TGC+++C N+    +G C+G GCC   + 
Sbjct: 127 ------NNKFTLVGCNALSLLSTFGK-QNYSTGCLSLC-NSQPEANGRCNGVGCCTTEDF 186

Query: 189 DIP-------NGLSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSS-FIDNFE 248
            +P        G   L   V   L   N S+ + N C YAF+V +  F F SS  + N  
Sbjct: 187 SVPFDSDTFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSSKDLKNLR 246

Query: 249 D-KEVEVVVDWSIGNETI-----IDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQ 308
           +     V +DWSIGN+T        +CG NS   +S +  R+ Y C+C +GY+GNPY  +
Sbjct: 247 NVTRFPVALDWSIGNQTCEQAGSTRICGKNSSCYNSTT--RNGYICKCNEGYDGNPYRSE 306

Query: 309 GCDQDINECEHKELNDCT--HECINTNGSYTCKCPKNYKGDGRRGEDGHGCTRDSKAIPI 360
           GC +DI+EC   + ++C+    C N +G + CKCP  Y  +           R    + I
Sbjct: 307 GC-KDIDEC-ISDTHNCSDPKTCRNRDGGFDCKCPSGYDLNSSMSCTRPEYKRTRIFLVI 361

BLAST of Cucsat.G6431 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 144.4 bits (363), Expect = 1.7e-34
Identity = 101/332 (30.42%), Postives = 154/332 (46.39%), Query Frame = 0

Query: 8   LVGLTVIILLSTLASAASQAKPDCDEWCGDLRIPYPFGVKQGCYF--NQAFLITCDKAFN 67
           LV +   +  + L     Q   +C   CG++ I YPFG+  GCY+  N++F ITC +  +
Sbjct: 9   LVAIFFSLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE--D 68

Query: 68  PPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIPNTTNLSAPATLPIA 127
            P      ++I V N + +G+L +L      CY++    +         NLS  A     
Sbjct: 69  RPHVL---SDIEVANFNHSGQLQVLLNRSSTCYDEQGKKTEEDSSFTLENLSLSA----- 128

Query: 128 DGKNKFIAIGCNTFGLFT--GMLKGGEFLTGCVAICTNNSIIVDGSCSGTGCCELDIPNG 187
              NK  A+GCN   L    GM     + T C+++C ++    DG C+G GCC +D+   
Sbjct: 129 --NNKLTAVGCNALSLLDTFGM---QNYSTACLSLC-DSPPEADGECNGRGCCRVDVSAP 188

Query: 188 LSDLSLAVGPVLPDTNRSLVKNNSCGYAFVVGEEGFKFKSS--FIDNFEDKEVEVVVDWS 247
           L   +            S    + C YAF+V ++ F F S+   ++        V++DWS
Sbjct: 189 LDSYTFETTSGRIKHMTSFHDFSPCTYAFLVEDDKFNFSSTEDLLNLRNVMRFPVLLDWS 248

Query: 248 IGNETI-----IDVCGINSKRNSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECE-- 307
           +GN+T        +CG NS      S  R+ Y C+C +G++GNPYL  GC QD+NEC   
Sbjct: 249 VGNQTCEQVGSTSICGGNS--TCLDSTPRNGYICRCNEGFDGNPYLSAGC-QDVNECTTS 308

Query: 308 ---HKELNDCTHECINTNGSYTCKCPKNYKGD 324
              H+        C N  G + CKC   Y+ D
Sbjct: 309 STIHRHNCSDPKTCRNKVGGFYCKCQSGYRLD 321

BLAST of Cucsat.G6431 vs. TAIR 10
Match: AT1G16260.1 (Wall-associated kinase family protein )

HSP 1 Score: 119.8 bits (299), Expect = 4.6e-27
Identity = 101/322 (31.37%), Postives = 145/322 (45.03%), Query Frame = 0

Query: 11  LTVIILLSTLASAASQAKP----DCDEWCGDLRIPYPFGVKQGCYFNQAFLITCDKAFN- 70
           L V++LL     AA+   P    +C + CG++ +PYPFG+ +GCY N+ F I C  + + 
Sbjct: 9   LVVMLLLRICEYAAASTFPLALRNCSDHCGNVSVPYPFGIGKGCYKNKWFEIVCKSSSDQ 68

Query: 71  PPKAFLKDTNISVTNISLNGELHMLQPIVRYCYEDVQLVSGTPFIP--NTTNLSAPATLP 130
            P   L     +VT+ +L G+   +    ++  +     SG P     ++++L+   +  
Sbjct: 69  QPILLLPRIRRAVTSFNL-GDPFSISVYNKFYIQSPLKHSGCPNRDGYSSSSLNLKGSPF 128

Query: 131 IADGKNKFIAIGCNTFGLFTGMLKGGEFLTGCVAICTNNSIIVDG---SCSGTGCCELDI 190
                NKF A+GCN       M   G  + GC   C N      G   SC G  CC++ I
Sbjct: 129 FISENNKFTAVGCNNKAF---MNVTGLQIVGCETTCGNEIRSYKGANTSCVGYKCCQMTI 188

Query: 191 PNGLSDLSLAVGPVLPDTNRSLVKN-NSCGYAFVVGEEGFKFKSSFIDNFEDKEVEVVVD 250
           P       L    V   T   L  N   C  AF+     F    S     E  E      
Sbjct: 189 P------PLLQLQVFDATVEKLEPNKQGCQVAFLT---QFTLSGSLFTPPELMEYSEYT- 248

Query: 251 WSIGNETIIDVCGINSKR-----NSSFSDDRSQYRCQCPDGYEGNPYLPQGCDQDINECE 310
            +I  E  +D+  + SKR     N+ F D    Y+C C +GYEGNPY+P GC QDI+EC 
Sbjct: 249 -TIELEWRLDLSYMTSKRVLCKGNTFFED---SYQCSCHNGYEGNPYIPGGC-QDIDECR 308

Query: 311 HKELNDC-THECINTNGSYTCK 316
              LN C   +C+N  GSY C+
Sbjct: 309 DPHLNKCGKRKCVNVLGSYRCE 311