Cucsat.G5169 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G5169
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Membrane metalloprotease ARASP, chloroplastic
Location	ctg1227: 4902299 .. 4904093 (-)
RNA-Seq Expression	Cucsat.G5169
Synteny	Cucsat.G5169

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCACACGCGACTGAGAAGCCTAAAAAACATACACACAAATACGAGGGGGAGAAAACAGGAAACACCTCTCTCAATCAATTCCCCTCCACAAATTTCAAAATGGTGTTATCCCAAACCTATTTCTTCTCCAATTCTTCCCTTTTCTTCCTCTATCTCTCTCTATAAGTCTCTGCCAATCCTGATATCCCACACCCAACTTTCAAAACCATGTTACTAAATCTCCCTTCCTCTTCTTTTTCTGTCATTAAGTTAGCTAATTTGAAGTCCCCTTTCTCTGATTTTTCAGTCAAGTCCAGAACCCACTTCTCTAAATCCTTATCATCATCATCATCTTCTTCTTCTTCTTCTTCATTGTGGCATTCACCTCCGTCGTCGGAACTTAGTTTCAAGGACAGACACCAAATTTTCTGGGACAAAACCAAAATCCGATGTCGGAGGAATGGCGATTTCAGGTTGTGGGCTTTTGCCGGAATCGATATTGGAAGTGCTCAGTCGGTACTTGAGGCGGCGGCGGTGTTGACGGCTATCATTGTTGTTCATGAGAGTGGACACTTCCTCGCTGCCTGTTTACAGGGTATTCATGTAAGTAAATTTGCAATTGGGTTTGGTCCAATTCTTGCGAAATTCAATAAAAACAATGTAGAATACTCTATTAGGGCTTTTCCTCTTGGTGGATTTGTGGGTTTTCCTGATAATGATCCTGACAGTGATATTCCAGTGGATGATGAAAATTTGCTTAAAAATAGACCGATATTGGATAGAGTGTTGGTGATTTCTGCTGGTGTTATTGCCAATATTATCTTCGCATACATAATTATCTTAGTTCAAGTCTCGTCCGTTGGTTTGCCTGTTCAAGAGCCGTTTCCAGGCGTTCTTGTGCCTGAGGTTCTAACTCTTTCAGCTGCTTCTCGTGATGGGCTGCTTCCTGGGGATGTGATTCTCGCTGTTAATGGAAATGAACTGCCAAAATTGGGTTCTACAGCAGTTTCTGATCTTGTTGAGGCAATTAAAAGAAGCCCCAATAGAACTGTGCTTCTCAAAGTTGAAAGAGGGAATCAGGATCTTGAAATTGGGGTCACCCCGGATAAGAGTTTTGATGGAACAGGGAGAATTGGGGTTCAGCTTTCACCTAATGTAAAGATTTCAAAAGTTGTAGCAAAGAATTTCTTGGAGGCTTTTAACTATTCTAGAAAGGAATTTTTGGGTCTTTCTTATAATGTTCTGGATAGCTTGAAACAAACTTTTCTGAACTTCTCTCAATCAGCCAGTAAGGTTTCTGGTCCTGTCGCCATTATTGCTGTTGGTGCAGAAGTTGCAAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAATATTAACCTTGCTGTTATAAACCTTCTTCCCTTGCCTGCTTTAGATGGCGGTTCGTTGGCTTTAATTCTCATCGAGGCTGTAAGGGGAGGGAGGAAGTTGCCTCTAGAACTCGAGCAGCGAATTATGTCATCTGGGGTCATGTTTGTTGTACTTCTTGGGCTATTCCTCATCATCCGAGACACGTTAAACCTTGAATTTATAAAGGACTTATTATGATCAGCCGATGCCTCAACTCGACAAGTAGTTTAACTCTGTTGAAACTGTAAATGGTTGGCTTGATGATTCTAGTGTTGGGGTTGTGGTTTTAGAGCTCATATGCTTTCATATTGTTGGATTCAGCTGATAATAGAGATAGAGAAAGAGGTCGTTTATAACAAAATGGAACCCTCTATTACATTCTTATCAGCTTTGTACTCCCAAATCAACAAGTCAAATTATACAAAACAGCTTATTCTTGTGCATCTTTCATGTTGCTTGAATCCATCTTCCTCTGTTGCAGGTAGAAAGAACTTGAGTTCAATATCCTTCCCTCATTTGCAACACATTTGAACTTCTTTTCTTTACATTTGTCTCTTAATAGAGTTTATGCAAAATAGTAGAGAAGGAAATGGTTCAAATTCAGTTGGAAGGCACAAAAAAGGAACCTGGCTAAAGTGTAAACTTGTGGGTTTGTTGGTCGAACTTTTTGGATATTAAGCAGAGAATGTTATGGTCAATTTGATTAAAGATTTGGGAGATGAATGGATGGGCCTTTTACTTCGTAATTGCAAGTAGTATTTTGGTAGATGAAGAAAAATTATTTGGAATCTATTTAAAAAATATTCCTGAAAATTTCAAACGTCTCATTAATACCTTTTACTTTTAAAGAATGCCCACAGGACAAGTTGTAGATGAAATTGTAAGTGATTAGGTTATAAATGTCACGAACTTTGGAAAGTTTAATAATATTTTTATACTTTTTTTTTAAAAAAGGAAGTTAAAATACTCATACTATTTGTATATTGAACATAATTAGCAAATACCTAGTTCCATAAATACTTTTGAACGACAGTTACTTTAACAAAAAGTGGGTGTAAAGTGTAAATGAAACTTAATAGTTTGTGTACACCAAGTTATACTCAACTTATGATCTAATGCTTCCCCTTCTTTCTCCCTCTCATTCAACGCCTTCTTACTCTTTTACTTCCATTCCATCACCACCAGGGACCTTAAGAGTGAAAACTAACCCAATTTATCAAGTTGTATCTTTCGTAGCTGAGTATGTGTTGAGGCGCAATTGGCCCATCATTAGAAAAAAGAATACAAAAGTTAGGCTCATAACTTCATGTTTTCAGATTTTATAAAAATGACAAAAGCACCAGTCCAACCAAATTGCTAAACTTAAAAAAGCCCCAGAAGCCTTTAAAAGTTTATTGATATACGATATATATTGACATGAAAGGTGTTTGATGTCTGATATACTACTGATGTGTTGATTGATATGTGATACTATTAACATTCTTGAAAATCACTTGATATGCGTTCTTGTACTGATGATATATACTATTGATACACTCCAAAGTTAGTTAATAAGTGATATGCAGGTGAAATACAATCGACGTTTTTAAGGTTGTTTAATATATGTATGGATATACCATTGATATACTTTTGATACATTGTAAAGTTGTTCCATGTTTGATATATTGTTGATATGCTAATGAAATTCTTGGAAGTTGAAAGATATGTGATATACTAATAACACATTATTGGCATTCTCTAAAAGGTATAGGGTTTAGCCTTTGTTTGATTTATAAGGGATTATTGTTGATTGATAACTAATTTTGTAAATATTTTGGTTCATTTTACTCTATATGAAAACAAATAAATATATAATAAACGGTGGAGATTGAAGGAGTGACGAAAAAGAGCCCAAAGAAGAGACGTAGCGAAGAGGAGAAACGAAGAAGGGTGAAGATGGAAGGAGTGAGGAAAAAGACGAGAAAGAGGAAGAGAAAAAGTGGCAAAAACATGAAAAATAAAAAGAAAAGAATGAAAAAGTGAGGAGAAATGGTTAAAATATAAAGAGAATGATGGAGAAGAGAATACTTAAATGGATTAAATTAAAGTTAATTGAAAAAATGAAATGATTGAAATAAAGTGAGAGAAAGAAGGAAACAAAAAAGTGAGAGGTTACCAACTCAATGGATTGGGATTTGATAAATTTGATATAATCCCTCCGTCAAACCAAATATTTTTGTTTCTATTAAACATTAAAATACATAATAGGAGAAGATGTCAACCGTCCATTCCTGATTCGAAAAGTCCTCTAAATCAACGCTCACAAATTCATCACACTATTTCCCTCTTTCTTAACGCATATTTATAAAATGCCTTCTCTGTCTCAAACACCTCTCTACTCAACTTCCCACAACAAATTCTCCCAACTCCGCTAACTGCAACAATAAAATATGAAGGCAAACACCATTCCCTCTTGGACTCCTCTTATCTTCTTCTTCATGTTCTAACTTCTTCAACCTCTATAACTGCTTCCAATTTCTTACCATTTTTTACCATGAATTTAGCCACTGCTATGGCCTTGGGTGATTCCTCGTCTTTGTGGTGTTTCATGCCTAAGTTGACGCCCTCTCCTCTCTCCTTCAAGTTGGATTTCAGAGGAGGGATTTTAGGATTGCCGCCTTCCTCAATGGCTTGCTCTATTGAGGCTGAGAAGCCACCCAATCGAACCACTGAGTTGCAGCCGGTTAACGATCAGGCCCATGGGAGTATTGCTCGGAAATTTAACAAGGATATGGCGTCTTTTCATAGTAAGTTTTGTTTTCTAATAAGGATATGTCGTTAGTTTGATATTGTATGAAACGTTGCGACTTGATTGTTTCGTTGATTCGTGTTGTGGAATTATTGTATGAATCTATTGGAATGCACATTAGGAGCAAATACTTCTGATTATGATAATGATCTGAAGCTGTTAGAGAATTTCTAGGAGCGACCGTTTTTAAAGTAAATTTAAAACCATTCGGATGAAGACGTGGAATGGTTCTGGTTAAGCTTCAAGTTTAGATATAGGTCAACGAGGAAACCGTGGTTGAAATGATTGGGCAGGAGGTCTAAGACTTGTTTGGGAGATTTTTACACAATAATATCTGAAGCAAAAGTTGAGAACATGTTTGGATAACTCTTTCTTTGAAATTGGTTACGGGAATACGTCCTTGTTGTGCTAACTTCAGTCAGAAAATCGATGTGGGAATGCAACCATTGACATGAATAAATTACTCCAACTGAACAGGGAGATTAGAACTAACACTAACAGAATCAGATGGTAAGTGAAATATATTTTTGTAAATCAACTCTTCAAGACCAAATATACTTTAAGCACAGCTTTGTGAAGCTATTCTTTCCGATTTGTGCTCCCTTAACATTGGCCTTTTCATTTTAACCTGTTATGTTTTCATCATAATTTGTTTAAATATTGTTTCGTCAACATTTCCTGCATCTAATGCATTTTCCATTTTATATAAATGGTTGCAGAACCATTATCTGTGTCAAATATATCGGCTTATCCAAATGATGATAGAAAGGTTCG

Coding sequence (CDS)

ATGTTACTAAATCTCCCTTCCTCTTCTTTTTCTGTCATTAAGTTAGCTAATTTGAAGTCCCCTTTCTCTGATTTTTCAGTCAAGTCCAGAACCCACTTCTCTAAATCCTTATCATCATCATCATCTTCTTCTTCTTCTTCTTCATTGTGGCATTCACCTCCGTCGTCGGAACTTAGTTTCAAGGACAGACACCAAATTTTCTGGGACAAAACCAAAATCCGATGTCGGAGGAATGGCGATTTCAGGTTGTGGGCTTTTGCCGGAATCGATATTGGAAGTGCTCAGTCGGTACTTGAGGCGGCGGCGGTGTTGACGGCTATCATTGTTGTTCATGAGAGTGGACACTTCCTCGCTGCCTGTTTACAGGGTATTCATGTAAGTAAATTTGCAATTGGGTTTGGTCCAATTCTTGCGAAATTCAATAAAAACAATGTAGAATACTCTATTAGGGCTTTTCCTCTTGGTGGATTTGTGGGTTTTCCTGATAATGATCCTGACAGTGATATTCCAGTGGATGATGAAAATTTGCTTAAAAATAGACCGATATTGGATAGAGTGTTGGTGATTTCTGCTGGTGTTATTGCCAATATTATCTTCGCATACATAATTATCTTAGTTCAAGTCTCGTCCGTTGGTTTGCCTGTTCAAGAGCCGTTTCCAGGCGTTCTTGTGCCTGAGGTTCTAACTCTTTCAGCTGCTTCTCGTGATGGGCTGCTTCCTGGGGATGTGATTCTCGCTGTTAATGGAAATGAACTGCCAAAATTGGGTTCTACAGCAGTTTCTGATCTTGTTGAGGCAATTAAAAGAAGCCCCAATAGAACTGTGCTTCTCAAAGTTGAAAGAGGGAATCAGGATCTTGAAATTGGGGTCACCCCGGATAAGAGTTTTGATGGAACAGGGAGAATTGGGGTTCAGCTTTCACCTAATGTAAAGATTTCAAAAGTTGTAGCAAAGAATTTCTTGGAGGCTTTTAACTATTCTAGAAAGGAATTTTTGGGTCTTTCTTATAATGTTCTGGATAGCTTGAAACAAACTTTTCTGAACTTCTCTCAATCAGCCAGTAAGGTTTCTGGTCCTGTCGCCATTATTGCTGTTGGTGCAGAAGTTGCAAGGTCCAACATTGATGGGCTCTATCAATTTGCAGCTGTGCTTAATATTAACCTTGCTGTTATAAACCTTCTTCCCTTGCCTGCTTTAGATGGCGGTTCGTTGGCTTTAATTCTCATCGAGGCTGTAAGGGGAGGGAGGAAGTTGCCTCTAGAACTCGAGCAGCGAATTATGTCATCTGGGGTCATGTTTGTTGTACTTCTTGGGCTATTCCTCATCATCCGAGACACGTTAAACCTTGAATTTATAAAGGACTTATTATGA

Protein sequence

MLLNLPSSSFSVIKLANLKSPFSDFSVKSRTHFSKSLSSSSSSSSSSSLWHSPPSSELSFKDRHQIFWDKTKIRCRRNGDFRLWAFAGIDIGSAQSVLEAAAVLTAIIVVHESGHFLAACLQGIHVSKFAIGFGPILAKFNKNNVEYSIRAFPLGGFVGFPDNDPDSDIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL

Homology

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BLAST of Cucsat.G5169 vs. ExPASy Swiss-Prot
Match: O23053 (Probable membrane metalloprotease ARASP2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ARASP2 PE=2 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 1.5e-116
Identity = 217/289 (75.09%), Postives = 254/289 (87.89%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           DIPVDD NLLKNRPILDRV+V+SAG++AN+IFAY II  QV SVGLPVQE FPGVLVP+V
Sbjct: 153 DIPVDDRNLLKNRPILDRVIVVSAGIVANVIFAYAIIFTQVVSVGLPVQESFPGVLVPDV 212

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
            + SAASRDGLLPGDVILAV+G EL   GS +VS +V+ +KR+P   VLL++ERG +  E
Sbjct: 213 KSFSAASRDGLLPGDVILAVDGTELSNSGSDSVSKVVDVVKRNPEHNVLLRIERGKESFE 272

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           I +TPDKSFDGTG+IGVQLSPNV+  KV  KN  E F+++ +EF GLSYNVLDSLKQTFL
Sbjct: 273 IRITPDKSFDGTGKIGVQLSPNVRFGKVRPKNIPETFSFAGREFFGLSYNVLDSLKQTFL 332

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQ+ASKV+GPVAIIAVGAEVARSN DGLYQFAA+LN+NLAVINLLPLPALDGG+LALI
Sbjct: 333 NFSQTASKVAGPVAIIAVGAEVARSNADGLYQFAALLNLNLAVINLLPLPALDGGTLALI 392

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 305
           L+EAVRGGRKLPLE+EQ IMSSG+M V+ LGLFLI++DTLNL+FIK++L
Sbjct: 393 LLEAVRGGRKLPLEVEQGIMSSGIMLVLFLGLFLIVKDTLNLDFIKEML 441

[Close]

BLAST of Cucsat.G5169 vs. ExPASy Swiss-Prot
Match: O80885 (Membrane metalloprotease ARASP, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ARASP PE=2 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 3.4e-113
Identity = 212/289 (73.36%), Postives = 252/289 (87.20%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           +IP+DDENLLKNRP LDR +V+SAG+IAN+IFAY II VQV SVGLPVQE FPGVLVPEV
Sbjct: 159 EIPIDDENLLKNRPTLDRSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEV 218

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
            T SAASRDGLL GDVILAV+G EL K G  AVS +V+ +KR+P   V+ ++ERG +D +
Sbjct: 219 KTFSAASRDGLLSGDVILAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFD 278

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           I VTPDK+FDGTG+IGVQLSPNV+I+KV  +N  E F +  +EF+GLS NVLD LKQTF 
Sbjct: 279 IRVTPDKNFDGTGKIGVQLSPNVRITKVRPRNIPETFRFVGREFMGLSSNVLDGLKQTFF 338

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQ+ASKV+GPVAIIAVGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALI
Sbjct: 339 NFSQTASKVAGPVAIIAVGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALI 398

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 305
           L+EAVRGG+KLP+E+EQ IMSSG+M V+ LGLFLI++DTL+L+FIK++L
Sbjct: 399 LLEAVRGGKKLPVEVEQGIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 447

[Close]

BLAST of Cucsat.G5169 vs. ExPASy Swiss-Prot
Match: P73714 (Putative zinc metalloprotease slr1821 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1821 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.6e-62
Identity = 131/289 (45.33%), Postives = 197/289 (68.17%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           +IP DD NLLKNRPILDR +VISAGVIAN++FAY +++ QVS++G   Q   PG+++P+V
Sbjct: 77  EIPADDPNLLKNRPILDRAIVISAGVIANLVFAYFLLIGQVSTIGF--QNIQPGLVIPQV 136

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
            + SAA   G+ PGD++L++ GN LP     A +  ++ ++RSP+  + ++V+RG +   
Sbjct: 137 DSASAAQVAGMEPGDIVLSLQGNTLPGF-PDATTQFIDIVRRSPSVPITVEVQRGEETKT 196

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           + +TP    +G G+IGV L PNV+  +  A N LEA  YS + F  +         Q   
Sbjct: 197 LTITPTPDAEGKGKIGVALLPNVETKR--ASNPLEALTYSAEAFERIVKLTTQGFWQLIS 256

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NF+ +AS+V+GPV I+  GA +ARS+   L+QF A+++INLAVIN+LPLPALDGG L  +
Sbjct: 257 NFADNASQVAGPVKIVEYGANIARSDASNLFQFGALISINLAVINILPLPALDGGQLVFL 316

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 305
           LIE +  G+ LP + +  +M +G++ ++ LG+FLI+RDTLNL F+++ L
Sbjct: 317 LIEGLL-GKPLPEKFQMGVMQTGLVLLLSLGVFLIVRDTLNLTFVQEFL 359

[Close]

BLAST of Cucsat.G5169 vs. ExPASy Swiss-Prot
Match: Q8YQ64 (Putative zinc metalloprotease all3971 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=all3971 PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.7e-51
Identity = 125/286 (43.71%), Postives = 185/286 (64.69%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGV----L 75
           DIP +D NLL+NRPILDR +VISAGVIAN+IFAY+++L QV  VG+  Q   PGV    L
Sbjct: 74  DIPPNDPNLLRNRPILDRAIVISAGVIANLIFAYMLLLAQVGFVGIG-QASQPGVSIQQL 133

Query: 76  VPEVLTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGN 135
            PEV   + A+  GL PGDVIL+ N  E        +  L + IK SP +++ L V RG+
Sbjct: 134 APEV--SAVATNAGLKPGDVILSANQKEF-GTSLQEIEALRDIIKNSPGKSIQLTVARGD 193

Query: 136 QDLEIGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLK 195
           + L + V P+    G G IG+ L+PN K+ +    +  +AF+    EF  +         
Sbjct: 194 ERLSVNVIPEAKPAG-GSIGIGLAPNGKVERRPV-SLSKAFSVGASEFQRIVVMTFKGFG 253

Query: 196 QTFLNFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGS 255
           Q   NF ++AS+V+GP+ I+ +GA +A+++   L+ FAA+++INLAVIN+LPLPALDGG 
Sbjct: 254 QLVTNFGETASQVAGPIKIVEIGANIAQNDTGSLFFFAALISINLAVINILPLPALDGGQ 313

Query: 256 LALILIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNL 298
           LA +LIE +R G+ LP  +++ +M +G++ ++ LG+FLI+++T  L
Sbjct: 314 LAFLLIEGLR-GKPLPNRIQEGVMQTGLVLLLGLGIFLIVKETTQL 352

[Close]

BLAST of Cucsat.G5169 vs. ExPASy Swiss-Prot
Match: O31754 (Regulator of sigma-W protease RasP OS=Bacillus subtilis (strain 168) OX=224308 GN=rasP PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 8.4e-27
Identity = 92/275 (33.45%), Postives = 141/275 (51.27%), Query Frame = 0


Query: 27  NRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGL 86
           ++P+  R+  I+AG I N I AY+I+++     G+P  EP  G L         A+  GL
Sbjct: 165 SKPVWQRIKAIAAGPIMNFILAYVILVMLGLIQGVPSNEPMLGQLTDN----GRAAEAGL 224

Query: 87  LPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTP----DK 146
             GD I ++NG ++      + +D+V A+K +P + + + V+R N+ L I VTP    D+
Sbjct: 225 KEGDYIQSINGEKM-----RSWTDIVSAVKENPEKEMDVAVKRDNKTLHISVTPEAVKDE 284

Query: 147 SFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSAS 206
           +    GR G        +   VA       + ++     LS  V    K   L       
Sbjct: 285 NKKTIGRFGSYAPTEKGVLSAVAYGATSTVDVTKAILTNLSKLVTGQFKLDML------- 344

Query: 207 KVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRG 266
             SGPV I  +  +VA++ I  L+QFAA L+INL ++NLLP+PALDGG L  + IEA+R 
Sbjct: 345 --SGPVGIYDMTDQVAKTGIVNLFQFAAFLSINLGIVNLLPIPALDGGRLLFLFIEAIR- 404

Query: 267 GRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNL 298
           G+ +  E E  ++  GV F++LL L +   D   L
Sbjct: 405 GKPINREKEAFVVFIGVAFLMLLMLVVTWNDIQRL 420

[Close]

BLAST of Cucsat.G5169 vs. NCBI nr
Match: KAE8652288.1 (hypothetical protein Csa_021982 [Cucumis sativus])

HSP 1 Score: 552 bits (1422), Expect = 6.24e-197
Identity = 303/321 (94.39%), Postives = 304/321 (94.70%), Query Frame = 0


Query: 1   MRVDTSSLPVYRVFI-----------------DIPVDDENLLKNRPILDRVLVISAGVIA 60
           MRVDTSSLPVYRVF+                 DIPVDDENLLKNRPILDRVLVISAGVIA
Sbjct: 1   MRVDTSSLPVYRVFMAFPLGGFVGFPDNDPDSDIPVDDENLLKNRPILDRVLVISAGVIA 60

Query: 61  NIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLPGDVILAVNGNELPKL 120
           NIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLPGDVILAVNGNELPKL
Sbjct: 61  NIIFAYIIILVQVSSVGLPVQEPFPGVLVPEVLTLSAASRDGLLPGDVILAVNGNELPKL 120

Query: 121 GSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTGRIGVQLSPNVKISKV 180
           GSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTGRIGVQLSPNVKISKV
Sbjct: 121 GSTAVSDLVEAIKRSPNRTVLLKVERGNQDLEIGVTPDKSFDGTGRIGVQLSPNVKISKV 180

Query: 181 VAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNID 240
           VAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNID
Sbjct: 181 VAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFLNFSQSASKVSGPVAIIAVGAEVARSNID 240

Query: 241 GLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVV 300
           GLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVV
Sbjct: 241 GLYQFAAVLNINLAVINLLPLPALDGGSLALILIEAVRGGRKLPLELEQRIMSSGVMFVV 300

Query: 301 LLGLFLIIRDTLNLEFIKDLL 304
           LLGLFLIIRDTLNLEFIKDLL
Sbjct: 301 LLGLFLIIRDTLNLEFIKDLL 321

[Close]

BLAST of Cucsat.G5169 vs. NCBI nr
Match: KAA0034835.1 (putative membrane metalloprotease ARASP2 [Cucumis melo var. makuwa])

HSP 1 Score: 533 bits (1373), Expect = 1.01e-188
Identity = 288/289 (99.65%), Postives = 288/289 (99.65%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV
Sbjct: 80  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 139

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
           LTLSAASRDGLLPGDVILAVNGNELP LGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE
Sbjct: 140 LTLSAASRDGLLPGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 199

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL
Sbjct: 200 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 259

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI
Sbjct: 260 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 319

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 304
           LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 320 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 368

[Close]

BLAST of Cucsat.G5169 vs. NCBI nr
Match: XP_004151933.2 (probable membrane metalloprotease ARASP2, chloroplastic [Cucumis sativus])

HSP 1 Score: 535 bits (1378), Expect = 4.05e-188
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV
Sbjct: 168 DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 227

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
           LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE
Sbjct: 228 LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 287

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL
Sbjct: 288 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 347

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI
Sbjct: 348 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 407

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 304
           LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 408 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 456

[Close]

BLAST of Cucsat.G5169 vs. NCBI nr
Match: XP_008455887.1 (PREDICTED: probable membrane metalloprotease ARASP2, chloroplastic [Cucumis melo] >TYK28920.1 putative membrane metalloprotease ARASP2 [Cucumis melo var. makuwa])

HSP 1 Score: 533 bits (1373), Expect = 2.41e-187
Identity = 288/289 (99.65%), Postives = 288/289 (99.65%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV
Sbjct: 169 DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 228

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
           LTLSAASRDGLLPGDVILAVNGNELP LGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE
Sbjct: 229 LTLSAASRDGLLPGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 288

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL
Sbjct: 289 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 348

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI
Sbjct: 349 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 408

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 304
           LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 409 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 457

[Close]

BLAST of Cucsat.G5169 vs. NCBI nr
Match: XP_038902635.1 (probable membrane metalloprotease ARASP2, chloroplastic [Benincasa hispida])

HSP 1 Score: 527 bits (1357), Expect = 6.10e-185
Identity = 285/289 (98.62%), Postives = 287/289 (99.31%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV
Sbjct: 167 DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 226

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
            TLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERG QDLE
Sbjct: 227 RTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGKQDLE 286

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           IGVTPD+SFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSY+VLDSLKQTFL
Sbjct: 287 IGVTPDESFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYSVLDSLKQTFL 346

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI
Sbjct: 347 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 406

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 304
           LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 407 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 455

[Close]

BLAST of Cucsat.G5169 vs. ExPASy TrEMBL
Match: A0A5A7T039 (Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold213G00770 PE=3 SV=1)

HSP 1 Score: 533 bits (1373), Expect = 4.87e-189
Identity = 288/289 (99.65%), Postives = 288/289 (99.65%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV
Sbjct: 80  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 139

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
           LTLSAASRDGLLPGDVILAVNGNELP LGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE
Sbjct: 140 LTLSAASRDGLLPGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 199

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL
Sbjct: 200 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 259

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI
Sbjct: 260 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 319

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 304
           LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 320 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 368

[Close]

BLAST of Cucsat.G5169 vs. ExPASy TrEMBL
Match: A0A0A0LT84 (PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G417840 PE=3 SV=1)

HSP 1 Score: 535 bits (1378), Expect = 1.96e-188
Identity = 289/289 (100.00%), Postives = 289/289 (100.00%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV
Sbjct: 168 DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 227

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
           LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE
Sbjct: 228 LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 287

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL
Sbjct: 288 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 347

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI
Sbjct: 348 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 407

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 304
           LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 408 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 456

[Close]

BLAST of Cucsat.G5169 vs. ExPASy TrEMBL
Match: A0A5D3E082 (Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3695G00250 PE=3 SV=1)

HSP 1 Score: 533 bits (1373), Expect = 1.17e-187
Identity = 288/289 (99.65%), Postives = 288/289 (99.65%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV
Sbjct: 169 DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 228

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
           LTLSAASRDGLLPGDVILAVNGNELP LGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE
Sbjct: 229 LTLSAASRDGLLPGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 288

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL
Sbjct: 289 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 348

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI
Sbjct: 349 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 408

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 304
           LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 409 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 457

[Close]

BLAST of Cucsat.G5169 vs. ExPASy TrEMBL
Match: A0A1S3C376 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495966 PE=3 SV=1)

HSP 1 Score: 533 bits (1373), Expect = 1.17e-187
Identity = 288/289 (99.65%), Postives = 288/289 (99.65%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV
Sbjct: 169 DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 228

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
           LTLSAASRDGLLPGDVILAVNGNELP LGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE
Sbjct: 229 LTLSAASRDGLLPGDVILAVNGNELPNLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 288

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL
Sbjct: 289 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 348

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI
Sbjct: 349 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 408

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 304
           LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL
Sbjct: 409 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 457

[Close]

BLAST of Cucsat.G5169 vs. ExPASy TrEMBL
Match: A0A6J1FWS5 (probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448141 PE=3 SV=1)

HSP 1 Score: 512 bits (1318), Expect = 2.04e-179
Identity = 274/289 (94.81%), Postives = 283/289 (97.92%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           DIP+DDENLLKNRPILDRVLVISAGVIANIIFAYIIIL+QVSSVGLPVQEPFPGVLVPEV
Sbjct: 161 DIPMDDENLLKNRPILDRVLVISAGVIANIIFAYIIILIQVSSVGLPVQEPFPGVLVPEV 220

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
            TLSAASRDGLLPGDVILAVNGNELPKLGSTAVS+LVEAIKRSPNRTVLLKVERG QDLE
Sbjct: 221 RTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSELVEAIKRSPNRTVLLKVERGQQDLE 280

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           IGVTPD+SFDGTGRIGVQLSPNVKISK+VAKNFLEAF+YSRKEFLGLSYNVLDSLKQT L
Sbjct: 281 IGVTPDESFDGTGRIGVQLSPNVKISKLVAKNFLEAFSYSRKEFLGLSYNVLDSLKQTVL 340

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           N SQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI
Sbjct: 341 NISQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 400

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 304
           LIEA RGGRKLPLELEQRIMSSG+M VVLLG+FLI+RDTLNLEFIKDLL
Sbjct: 401 LIEAARGGRKLPLELEQRIMSSGIMVVVLLGMFLIVRDTLNLEFIKDLL 449

[Close]

BLAST of Cucsat.G5169 vs. TAIR 10
Match: AT1G05140.1 (Peptidase M50 family protein )

HSP 1 Score: 420.6 bits (1080), Expect = 1.1e-117
Identity = 217/289 (75.09%), Postives = 254/289 (87.89%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           DIPVDD NLLKNRPILDRV+V+SAG++AN+IFAY II  QV SVGLPVQE FPGVLVP+V
Sbjct: 153 DIPVDDRNLLKNRPILDRVIVVSAGIVANVIFAYAIIFTQVVSVGLPVQESFPGVLVPDV 212

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
            + SAASRDGLLPGDVILAV+G EL   GS +VS +V+ +KR+P   VLL++ERG +  E
Sbjct: 213 KSFSAASRDGLLPGDVILAVDGTELSNSGSDSVSKVVDVVKRNPEHNVLLRIERGKESFE 272

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           I +TPDKSFDGTG+IGVQLSPNV+  KV  KN  E F+++ +EF GLSYNVLDSLKQTFL
Sbjct: 273 IRITPDKSFDGTGKIGVQLSPNVRFGKVRPKNIPETFSFAGREFFGLSYNVLDSLKQTFL 332

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQ+ASKV+GPVAIIAVGAEVARSN DGLYQFAA+LN+NLAVINLLPLPALDGG+LALI
Sbjct: 333 NFSQTASKVAGPVAIIAVGAEVARSNADGLYQFAALLNLNLAVINLLPLPALDGGTLALI 392

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 305
           L+EAVRGGRKLPLE+EQ IMSSG+M V+ LGLFLI++DTLNL+FIK++L
Sbjct: 393 LLEAVRGGRKLPLEVEQGIMSSGIMLVLFLGLFLIVKDTLNLDFIKEML 441

[Close]

BLAST of Cucsat.G5169 vs. TAIR 10
Match: AT2G32480.1 (ARABIDOPSIS SERIN PROTEASE )

HSP 1 Score: 409.5 bits (1051), Expect = 2.4e-114
Identity = 212/289 (73.36%), Postives = 252/289 (87.20%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           +IP+DDENLLKNRP LDR +V+SAG+IAN+IFAY II VQV SVGLPVQE FPGVLVPEV
Sbjct: 159 EIPIDDENLLKNRPTLDRSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEV 218

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
            T SAASRDGLL GDVILAV+G EL K G  AVS +V+ +KR+P   V+ ++ERG +D +
Sbjct: 219 KTFSAASRDGLLSGDVILAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFD 278

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           I VTPDK+FDGTG+IGVQLSPNV+I+KV  +N  E F +  +EF+GLS NVLD LKQTF 
Sbjct: 279 IRVTPDKNFDGTGKIGVQLSPNVRITKVRPRNIPETFRFVGREFMGLSSNVLDGLKQTFF 338

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
           NFSQ+ASKV+GPVAIIAVGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALI
Sbjct: 339 NFSQTASKVAGPVAIIAVGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALI 398

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 305
           L+EAVRGG+KLP+E+EQ IMSSG+M V+ LGLFLI++DTL+L+FIK++L
Sbjct: 399 LLEAVRGGKKLPVEVEQGIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 447

[Close]

BLAST of Cucsat.G5169 vs. TAIR 10
Match: AT2G32480.2 (ARABIDOPSIS SERIN PROTEASE )

HSP 1 Score: 349.4 bits (895), Expect = 3.0e-96
Identity = 190/289 (65.74%), Postives = 226/289 (78.20%), Query Frame = 0


Query: 16  DIPVDDENLLKNRPILDRVLVISAGVIANIIFAYIIILVQVSSVGLPVQEPFPGVLVPEV 75
           +IP+DDENLLKNRP LDR +V+SAG+IAN+IFAY II VQV SVGLPVQE FPGVLVPEV
Sbjct: 159 EIPIDDENLLKNRPTLDRSIVVSAGIIANVIFAYAIIFVQVLSVGLPVQEAFPGVLVPEV 218

Query: 76  LTLSAASRDGLLPGDVILAVNGNELPKLGSTAVSDLVEAIKRSPNRTVLLKVERGNQDLE 135
            T SAASRDGLL GDVILAV+G EL K G  AVS +V+ +KR+P   V+ ++ERG +D +
Sbjct: 219 KTFSAASRDGLLSGDVILAVDGTELSKTGPDAVSKIVDIVKRNPKSNVVFRIERGGEDFD 278

Query: 136 IGVTPDKSFDGTGRIGVQLSPNVKISKVVAKNFLEAFNYSRKEFLGLSYNVLDSLKQTFL 195
           I VTPDK+FDGTG+IGVQLSPNV+I+K                                 
Sbjct: 279 IRVTPDKNFDGTGKIGVQLSPNVRITK--------------------------------- 338

Query: 196 NFSQSASKVSGPVAIIAVGAEVARSNIDGLYQFAAVLNINLAVINLLPLPALDGGSLALI 255
               +ASKV+GPVAIIAVGAEVARSNIDGLYQFAA+LNINLAVINLLPLPALDGG+LALI
Sbjct: 339 ----TASKVAGPVAIIAVGAEVARSNIDGLYQFAALLNINLAVINLLPLPALDGGTLALI 398

Query: 256 LIEAVRGGRKLPLELEQRIMSSGVMFVVLLGLFLIIRDTLNLEFIKDLL 305
           L+EAVRGG+KLP+E+EQ IMSSG+M V+ LGLFLI++DTL+L+FIK++L
Sbjct: 399 LLEAVRGGKKLPVEVEQGIMSSGIMLVIFLGLFLIVKDTLSLDFIKEML 410

The following BLAST results are available for this feature:

BLAST of Cucsat.G5169 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23053	1.5e-116	75.09	Probable membrane metalloprotease ARASP2, chloroplastic OS=Arabidopsis thaliana ...	[more]
O80885	3.4e-113	73.36	Membrane metalloprotease ARASP, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
P73714	3.6e-62	45.33	Putative zinc metalloprotease slr1821 OS=Synechocystis sp. (strain PCC 6803 / Ka...	[more]
Q8YQ64	1.7e-51	43.71	Putative zinc metalloprotease all3971 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82...	[more]
O31754	8.4e-27	33.45	Regulator of sigma-W protease RasP OS=Bacillus subtilis (strain 168) OX=224308 G...	[more]

BLAST of Cucsat.G5169 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8652288.1	6.24e-197	94.39	hypothetical protein Csa_021982 [Cucumis sativus]	[more]
KAA0034835.1	1.01e-188	99.65	putative membrane metalloprotease ARASP2 [Cucumis melo var. makuwa]	[more]
XP_004151933.2	4.05e-188	100.00	probable membrane metalloprotease ARASP2, chloroplastic [Cucumis sativus]	[more]
XP_008455887.1	2.41e-187	99.65	PREDICTED: probable membrane metalloprotease ARASP2, chloroplastic [Cucumis melo...	[more]
XP_038902635.1	6.10e-185	98.62	probable membrane metalloprotease ARASP2, chloroplastic [Benincasa hispida]	[more]

BLAST of Cucsat.G5169 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T039	4.87e-189	99.65	Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A0A0LT84	1.96e-188	100.00	PDZ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G417840 PE=3 SV...	[more]
A0A5D3E082	1.17e-187	99.65	Putative membrane metalloprotease ARASP2 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A1S3C376	1.17e-187	99.65	probable membrane metalloprotease ARASP2, chloroplastic OS=Cucumis melo OX=3656 ...	[more]
A0A6J1FWS5	2.04e-179	94.81	probable membrane metalloprotease ARASP2, chloroplastic OS=Cucurbita moschata OX...	[more]

BLAST of Cucsat.G5169 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05140.1	1.1e-117	75.09	Peptidase M50 family protein	[more]
AT2G32480.1	2.4e-114	73.36	ARABIDOPSIS SERIN PROTEASE	[more]
AT2G32480.2	3.0e-96	65.74	ARABIDOPSIS SERIN PROTEASE	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001478	PDZ domain	SMART	SM00228	pdz_new	coord: 209..283 e-value: 5.7E-11 score: 52.4
IPR001478	PDZ domain	PROSITE	PS50106	PDZ	coord: 211..282 score: 11.4408
IPR041489	PDZ domain 6	PFAM	PF17820	PDZ_6	coord: 226..281 e-value: 1.6E-8 score: 34.3
IPR036034	PDZ superfamily	GENE3D	2.30.42.10		coord: 211..307 e-value: 2.4E-16 score: 61.8
IPR036034	PDZ superfamily	SUPERFAMILY	50156	PDZ domain-like	coord: 213..293
IPR008915	Peptidase M50	PFAM	PF02163	Peptidase_M50	coord: 101..437 e-value: 9.2E-61 score: 205.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..55
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..57
None	No IPR available	PANTHER	PTHR42837:SF4	MEMBRANE METALLOPROTEASE ARASP2, CHLOROPLASTIC-RELATED	coord: 20..456
None	No IPR available	CDD	cd00989	PDZ_metalloprotease	coord: 209..292 e-value: 2.80826E-15 score: 68.7995
None	No IPR available	CDD	cd06163	S2P-M50_PDZ_RseP-like	coord: 97..448 e-value: 5.93803E-42 score: 145.249
IPR004387	Peptidase M50, putative membrane-associated zinc metallopeptidase	PANTHER	PTHR42837	REGULATOR OF SIGMA-E PROTEASE RSEP	coord: 20..456

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G5169.T1	Cucsat.G5169.T1	mRNA
Cucsat.G5169.T2	Cucsat.G5169.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0005515	protein binding