Cucsat.G5051 (gene) Cucumber (B10) v3

Overview
NameCucsat.G5051
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptiontRNA (Cytosine(38)-C(5))-methyltransferase
Locationctg1227: 2618484 .. 2625481 (-)
RNA-Seq ExpressionCucsat.G5051
SyntenyCucsat.G5051
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTTAGACGACCCACGGTAGTCTCAACCCGACTGCTGGACAAAGATTGGGTGCCGGCGGGGCGGTATAATCAGGCGTAAATGGAGAAGGTTTTTCCGAAGCTGCAAGACGAACCATGGAGAGTCCTCGAGTTCTACAGCGGCATTGGTGGCATGGTACTCTTTTCCCCTACGTCTCTCTCTGCCTTCATCTCTTTCTCGGGCGCACATCTTTACATAGCCGTTGTTTGTGCATTGAATTATAACAGAGGTACTCGCTACTGAGTGCTGGCATCCATGCGAAAGTGGTACAAGCTTTTGATATTAACGACAAAGCAAACGATGTATACGAACACAATTTCGGTGATCGTCCATACCAGGTATGGCAGAATCTTCTTCTTTATCTCGTGTTTAGAACGATTTGCTTTCTACTTCAGGGTTATGTTTTTGCTGCTTCTCATTAGAAACAACGGAATTGAATAAATCACCTCCAGTGACGTTTGAAGTAAACTGAATCTCAAATTGTCGAGTATTGGAACTAAAGCTTTTGTAGTTTGTGAGAAATTAGTTCCTGCACAATGGAGGATATACTTTTGAAGTGATAAGAATAAAGATTACGATGTATAGCTAGTATGAATTCACAACGTAAGCGGACAGTAGGATAGGTATATGAAGTGTTTTTGTTGGAATATCTGATTCAAATGTCTACAGCTGCGGAGTTCCTTTTGAAATTTCAAAGCAAAAGCGTCCTAAATCCTGTTGATATTGTTTTCCCCATTATATTTATTGTCCCGAAAACAATTCCATTATGTTTCTGTAGGAGGTTAGTCTTGCTGCTTCTCAGTACAGATAACGAAACTACTCTGTTCATTCAGAAATGAGATGCTAATTCAATTCTTCTCGATCGTGTTTTAAGTAGTAGATTGAAGTTGAGGTTATTGAATGTCGAAACCAAGCTTGATCTATCTTGTTGGAAATTACATCACTCTTTCTGATGAACGTACTTTTATAGCGATATGATTACAATTTAAATATAGCATGAACTCACAGCGTAGTGGAGAATATGGTAACTATATGAAGCATTTGTGTTGGAATCTCTCATTCAAATGACTTCTGTTGTGGATTCTTCTATTTATTTATTTCCTTTTGAATTGTCAAACTATAAGCGTTATACAAACCAGGACATGTAACGGTTCAGGCTAGACTTCCTTTGAGTAGGAGTGTTTTTTTTTTTTTCTTATACGAGTTGTGGTCAAAAGTGATCCTGTGTGCTTGGTTACGTTGAGCTACTGGTAAGATTCAGAATGAAAAAGGAACTACAAGAAGGACTAAAAATTGAGTCTAAAAAAGGGATGACAGTCCTTGAATTTTACAACAACACAGGTGGCATGGTGGCTTTAATTGAGTTCTCATTTTCCCTGGTCTGCCATCCTTAGTTCTTGGATATATTATCAAATTTGGACTCTAGACATTTAAGATACTGAAGAAATTTTCTTTAACTAGGAGTGTTGGTTTTTCCTTATAAGCTTAGTTATGGTCAAAAGAAACCTGTGTCCTTGGTCTAGCTAATGGTAAGATTGAGAAAGCAAATGAGAGCTATTAGAAATACACCAAACTTAAAATTAAAAAGCAAGCCCTGGAGAGTCCTTGACTTCTCATTATTCCCAACTACATAGGTCCAGCATGGTAGCATCCTTAGTTTTTGGTTCTATAATCTAATTTGGAGTCTAGATGTTTAGGATCCTTAGAAAAAATCATCTATAATAGTAAATTTCTATTTGACATAAGTTTCATCATCCCGTTCCTCTAGTATCTTCATCAAACACCATGCTTAGAGATCTTTACCATTTCCCTATTTGATCATCACCTCAACTTGTATGTCACAATCAAAATTTAGCTGTAGTTACTTGATCCTTGAATATGTGATGGGTCTTGGAGGCAAGGCAGGAATATCCCAGTGCATATCAAAAGTCACTAGAGATTTGATCCCTTTTAAATTATTGCCTTTGCAGTAAAACTAAAGTTAATCTGCTGCTTCAACTCAACTAATGGACCAATGTCATTTGCCATACGTAGTTTAGTTATTATGTTCTTTATTTGGAACTCGAGAAATAGGAAAAGCTGTTCGTATTAGTAGGCAAGGCAAGAGCAAGACCTTTACTAGGCTTGCCTTGGTCACTCTTTCTAACCACACTGAAGAGTTGTGTAATTGAACAGCTGGAGCGCCTTTTTCTCCTCTCCTTATCCTTGGTTTGTGCTAAATTAGATCACTTCACTCCTAATTCCTGTGGGTGCAATAAATTGACCACCGGCACTAAGTTCAGGTGGGCGAAGAGATGATATGAATTTTTGTAATGTTGATGTAATGCAAATGTTTGATTCAGTTATCTTTGTGCAAGTTTATCTGCATTTGGAATCAAAGCTAACATTTATTAGGTTGTTATCTTTGGCAAGAACAAAACCGAGCATACAGCTTGACATTATTCAACAACTAGTGATATATTGGTTTCTATTTTAGGCTGCTTCAGTTTGCATAAAACTGGGATAAACTTTGATATGTTAAAATAATTTGGCCATGTCAGTCTTTTCTAAGCACTTTATTTGGATAGGGTAACATTGAAAGTCTGACTGCTGCTGATATCGACAGCTACCAAGCTCATGTTTGGCTTCTTTCACCTCCTTGCCAACCATATACTAGACAAGGTATGTTTTACAGATGATTGATTGTAAATAAATAGTTTATATTGTCTACAGTTTTGTAAACTTCGTAATTCGTAAAGCACGGTGATAAAGTGAGAAATTTCTACTTGTCAACTATCATTGGGGGAACATGCTTTGGAATTGAACTAGTTATATAGTAGAGACTTTACATTCTAATTTCGAGAATATTCTTTTACAAAGTTGAGTTTCTTAGTTAATTTTCCGTAAATTATTTTAAATGATTTTCCATTCTCACTTGAATACGGCAGGAGTCATGTTAAGCATAAACGGGAGATTCATGAGAAGCGAAGTGTACAGTAAAATTTTGTTAAATATACTTTTTCAATGATGAAATATATTTAGACAATTTATCTAAATATATCTGCAGCAATATATAAAATATTAAATTGTGAAATGATATTGGAATTCTTAGACGATATCAAGGAAATACTGCTCATTTTTTTCCATGTTTTAACCAATTATTTCATAACTTCTTGCAGTTAATTGCCTTTTCTTTTCATCTTTTATCCATAACAGAGTAACAGTGTTGTAAAAGTTACATCCTAAATAGAATTTCATTGATTTATAAGCTCCAGCCAAAATATACAAACACACGCTCACACCATTACCTGATTTACAAGCCTTTCACTTGAACAATCTTCCTGCTACATTTTTATTCATGTACATCATACATTTTATAAAGTGGAAAGTGGGTAATTGTGCACCATGTAATTAGGTCTACAAAAGCAATCTGGCGATGCTCGGGCATTTTCATTCCTCAAAATTCTTGAGTTGATGGTAGAAGCATCGAGGCCACCAGTAATGTTATTCATGGAAAACGTTGTCGGCTTTGAGGTATGTTGCTTGAACTTATCTGGGCACTGGAATTTATTTGACTGATTCTTCATCATATTTTAGATGCCTTCCTTTTTGTTGCAGACATCTGACACACATACAAAAATGATTGAGATATTGGAAAAGACTGGTTTTTCTACACAAGAGTTTATTTTAAGCCCAATGCAATTCGGTGTCCCATATTCCAGACCGCGGTACTTCTGTCTGGTAGCATACATCCCTCGCTTCCATTTCTCTTTGTTCTTACCTAGCTTTTATTTTATTGTTAAGCGCTTTGCTTTCCTATTTAATTTCCCCAATATGACAATTTCATGGAGCTTTTTATTCCATCGAATAACATTCCTTGTTTCAGTAGCTATGTGGTTTAACATTCTGTCCTTGATGATTTGCACCCTCAACCTTACAGACCACCATGGGTTGGCAATAGTAAAGGTCTTAGTGAGAGTGGGTTTAGATTAAGCCGTGCCACAAATTACCTTGTCATCTAAATGTTGTTGGATCAAGTACATGAGAATAGTATGGATGTGGTCGTACTAACCTAGGCATTCATGGATAAAATAAAAATAAAAATCACGTCAAATGTTTCACAAAAGAGCACCATTGAGTTTTCCTGATGGCACCAACTGACCTACCAAATATTGAAACTGCTGAAAATTTCCCATAACAAAACTGAAAAAAGGGTTTTGATTGTACGACCACCATCCTTATCTTGATAAATGCAATATCACAACTGCCACAAAAGATTAAAATAAAGGAAAGCATGGAAATACTTAGCTAATATACGTGGTAGCTTGAGGAAGAAGGGAGGTGGAGATTCAATTCCTTGTTTCCTGATGTTGTGGATTTTCAAGTTTTTTATTAGTTTATGCCTACTTCAACAACAATGAAGAATGAGATAGTGAAGAACGAAACAAAGGAACATATGTATTTGTGGTATTTTGTTTTGGGGCCAGGTCTTTAATGAATAAGCAAGTAAATGTACTGCATGATCCAATAAAGTGGAGGGAAAATAGTCGTTTTGCCATGCACTGAAGATAGACCTAGACGATGCTACATAAAGATTATAATTGCTAACCATTTTTCTATGTTAGCACAACAATCCAAGGACCGTTTAAAACCCTTCCCTAAACTTTAGGCTAAAAATTCACTTTTAATGGGGTTAAAATTCTTTAAATCATTTTTTTATTCACTTCATGCTTAATATCCATCTAAAACCATTAAAGATATGGTGGTAAAAAATGAGACATAGTCTCTACTAACTTAGAGGTCATAGGTTCAATCCATAGTAACCACCTACCTAGAATTAATTTCCTTCGACTTCCTTGACATCCAAATGTTGTAGGGTTAGACAGATTGTCTTGTAAGATTAGTTGATGTGTGCATAAATTGGCCTGAACATTCGTAAATATAAAGAATTTAAAAAAACCATTAAAGGTACTGTCACACCAATCCCAACATAATGGAGATTGATGCATTTCTTTGTTGAGTGCTCTGATACAAACCAAAACATAATCAACTATTTTGAAACATAAATAATTCGACGTATTTGTGAAGCCTTCCCTCTTGACATCACTCGAGCCCTATACCAATTAATCAATGTTTACATACTTCCTTCCCCTTCGACTCTATTTATAACCAAATATCATAAGGACCTCCCTAAGCAATTGCTAACAAAACTTTATATCCTAATAACACCTTAACACCATTTCTATGATACCCTTTTGCTGGTTCTTTCTAGACGCTCTGCCTGTCGTTGCTCAGTTGCCCCACTGCGATGCCTGGAGTTCTTCTGACCTTGATGGAATCTATTCATATTAATCACCCATTTGGCAAAGAAAATGAATGCTTTGATTAAATTCACTATGGCTTTTTTCTGGTTGATTGGCTGAGGACAGTGAGGAGAAATCATGAATCTTCGATGACAAAGGCCACCGGTTTGGAGATTTTTTAAAAAACATTTTTTTCAATGACCACAATTATAACAGTGGTTTATGTTCAATTTGTTGAACTGTCGGTACATGACATAGCATGGATCTCATCTACGTTATTATTTTTCTGTGAATCAAGTGTTTCAAGCAGAGATCTTATCATTTGTGTTTTAATATATTTTAGGGAAAATGTGTTTCTTACTTACCTTATATGGGGTCCTTGTACCTTTGCAGGCCAAAAGGAAGCCTTTATTCTTTGAGAAGGAATTATATAATAATCAACTTCTTTGGTCTCCAAACCCATTACTTGATAGTGATGCTAACTTAGAAAGCATTGAAACTCATGGATCCCAAGCAACTTCTGATAAGTTATTGCTCTCTTGCGAGCCAATATGTAGGTTTCTTGACCATTCCAACCATCAGGAGTTAAGTTTTTATGATGCCAGTAGTATTCAAAGTGAGGTTGCATTGGAGAAAAACAAGGATGCCGATGAGCAAGTTATTGAACCTTTTAATCAATATCTCGTCCCATCCAGTTTGATAGACAGATGGGGCAGTGCTATGGGTATCCAGAAATATTTTATGTTTAATCGTAATTGTTATAGTGTTAGTATGATCTGTATAGTTTTATCAGTGCTCACTGTAGACTATAGACTATATCCTTAAAACAGTTTAGATATTGTATATCCTGATTCAAGGCGATGCTGCTGTTTCACAAAAAGCTACTACCGATATGTTAAGGGCACAGGCTCGCTTTTGGCACCTTTTGAGGTAAAATTTTATCCTTGACATAATAGTTTGGAATATATAGTCTTCTACATCTTGTGCAGGGTACAAAAGAGTTGCAATTTTTCTTACTTTTGTTACTGACGGCTTGTAATGCAGACTGAAGGGATGGGCAAGGCTCATTCTCTTAAAGAGCAGAAGCTCAGATATTTTACTCCTAGAGAGGTACGGGATGACTTTTATATAAAATGATTAATTCTTCGTTAGTTTTCTGCTTCAAGCTGATGCTGTTAATTAATCCGTAGAATTTGTTCTTTTTTTTTTATTTATTTATCAAATTTTAGGTTGCTAACTTGCATTCCTTTCCGGAAGATTTTCAATTTCCCCAACACATCGGTCTTAGACAACGGTATGTATTTCATTTTCCCTTGGACCGCCCACCTCCCTTTCTCATTACTTGTAGGTTCAACTCTGGAGGCCTCTTTTAGTCACATTTGTTAGGATGACAAAACGATGAGTTTTTCTTCCACTGTTTGGACCTTTCTCATTAACTCCAAAAGAACACTTGATCTGACTGGCTTTTTGGACCTTTCTCATTTACTCCTTTGATAATATAAACATAGGAAACAAACAGTTAACTACCAGATTTCTGATGGCTTTGACAATTCATCACTCATCAGCTGGGTTAGATTTCATGAGATGAAAATTCAAGTCATTAGAAAGTGAGTTTCGAAATTGGTAGGGCATGTTTAGAAGTTTGTTTGTAATACGAAAGCAATGTTAGGTCCAAAATCACCTCAAATTTTCAAAATCAAATTTGGCATTAGACTTTCCATTTGCTTTGCTCAATATTTTTCTTTTTTTTTTAGAATGATCGAGAAAGCATATTTTGAAGTGATTTTGATGAAAAGTCTCTAAACACACGTGCACTTGATTCAGCAGTCTTTGAAGTTTGTGAAGTAAAATTTTGTTTTTCTCCTCTACCGTTTCAAACTATCAAAATAGTTACACGGGTATTTGCTTTCTCTTTTGCAGTTACGCACTACTAGGGAACAGTTTGAGCATTGCCGTTGTTGCTCCCTTGCTCAGATATCTCTTTACTGAGCCATCTTGACCAAGAGAGCGATTGGATGTCAATACTCTATTCAATCTTCTACTAAATTTTGTATTAAAAGAATCCATTTTTAGTTAATGGAGAGTAATATAATACTTCTTGCTTTGTTCGAAATATTTACTATTACCGATAAAACAAAGAAAGTGCAAATGCTATTCATTTATCAAAATTGTAAACCGATAACAGTAGTTAAAATATATCAAACCATAACCAAAACCAAAACCTACTATGAAACAGACATAGCGAGACACAACCAACCGACCCGTACGAACCACAATCACTATACGGCAAAAGCTTGCCCCAAATGCACGCCCTTTGTATAAAATAAGGCACTTAGTCAAAAGCATCGTCATCTTCGTCAGGAAGAGGTTGTACAGCAGCTTGCGCCAACTCTCGTTCATGC

Coding sequence (CDS)

ATGGAGAAGGTTTTTCCGAAGCTGCAAGACGAACCATGGAGAGTCCTCGAGTTCTACAGCGGCATTGGTGGCATGAGGTACTCGCTACTGAGTGCTGGCATCCATGCGAAAGTGGTACAAGCTTTTGATATTAACGACAAAGCAAACGATGTATACGAACACAATTTCGGTGATCGTCCATACCAGGGTAACATTGAAAGTCTGACTGCTGCTGATATCGACAGCTACCAAGCTCATGTTTGGCTTCTTTCACCTCCTTGCCAACCATATACTAGACAAGGTCTACAAAAGCAATCTGGCGATGCTCGGGCATTTTCATTCCTCAAAATTCTTGAGTTGATGGTAGAAGCATCGAGGCCACCAGTAATGTTATTCATGGAAAACGTTGTCGGCTTTGAGACATCTGACACACATACAAAAATGATTGAGATATTGGAAAAGACTGGTTTTTCTACACAAGAGTTTATTTTAAGCCCAATGCAATTCGGTGTCCCATATTCCAGACCGCGGTACTTCTGTCTGGCCAAAAGGAAGCCTTTATTCTTTGAGAAGGAATTATATAATAATCAACTTCTTTGGTCTCCAAACCCATTACTTGATAGTGATGCTAACTTAGAAAGCATTGAAACTCATGGATCCCAAGCAACTTCTGATAAGTTATTGCTCTCTTGCGAGCCAATATGTAGGTTTCTTGACCATTCCAACCATCAGGAGTTAAGTTTTTATGATGCCAGTAGTATTCAAAGTGAGGTTGCATTGGAGAAAAACAAGGATGCCGATGAGCAAGTTATTGAACCTTTTAATCAATATCTCGTCCCATCCAGTTTGATAGACAGATGGGGCAGTGCTATGGGTATCCAGAAATATTTTATGTTTAATCGTAATTGTTATAGTGTTAGTATGATCTGTATAGTTTTATCAGTGCTCACTGTAGACTATAGACTATATCCTTAA

Protein sequence

MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRPYQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRPPVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPLFFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELSFYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGIQKYFMFNRNCYSVSMICIVLSVLTVDYRLYP
Homology
BLAST of Cucsat.G5051 vs. ExPASy Swiss-Prot
Match: F4JWT7 (tRNA (cytosine(38)-C(5))-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=DNMT2 PE=1 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.7e-92
Identity = 172/280 (61.43%), Postives = 212/280 (75.71%), Query Frame = 0

Query: 9   QDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRPYQGNIESL 68
           + +PW+VLEFYSGIGGMRYSL+++GI ++VV+AF+IND ANDVY+HNF  RPYQGNI+SL
Sbjct: 11  EKKPWQVLEFYSGIGGMRYSLMASGIVSEVVEAFEINDSANDVYQHNFKHRPYQGNIQSL 70

Query: 69  TAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRPPVMLFMEN 128
           TAAD+D Y A  WLLSPPCQPYTRQGLQK SGDARAFSFL+ILEL+   ++PP MLF+EN
Sbjct: 71  TAADLDKYNADAWLLSPPCQPYTRQGLQKHSGDARAFSFLRILELIPHTTKPPQMLFVEN 130

Query: 129 VVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPLFFEKELYN 188
           VVGFETSDTH +MI  L K  + TQEFILSP+QFGVPYSRPRYFCLAKRKPL F+ +  N
Sbjct: 131 VVGFETSDTHMEMIGTLTKLDYVTQEFILSPLQFGVPYSRPRYFCLAKRKPLPFKSQHSN 190

Query: 189 NQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNH--QELSFYDASS 248
           N+LLWSP+PL   D  +E  +    +   DKLL  C+P+ +FL+ + H   E S  D S 
Sbjct: 191 NKLLWSPDPLYGRDDQVEFGKCQAEEGL-DKLLEFCKPVEKFLELAAHVDGEPSSVDDSE 250

Query: 249 IQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGI 287
             S+    +  D+   V +  +QYLVP SLI+RWG+AM I
Sbjct: 251 NGSKDCCGQEGDS---VPDSVHQYLVPVSLIERWGNAMDI 286

BLAST of Cucsat.G5051 vs. ExPASy Swiss-Prot
Match: Q54JH6 (DNA (cytosine-5)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=dnmA PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.1e-41
Identity = 105/294 (35.71%), Postives = 158/294 (53.74%), Query Frame = 0

Query: 11  EPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRPYQGNIESLTA 70
           E  RVLEFYSGIGGM Y L  +G+  +V+Q+FDIN  AN  Y++ F +   Q +IES + 
Sbjct: 2   EQLRVLEFYSGIGGMHYGLQESGVDFQVIQSFDINTNANLNYKYTFNEDSSQKSIESYSV 61

Query: 71  ADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRPPVMLFMENVV 130
            +++ ++A+ WL+SPPCQP+TR GLQK   D R  SF  +L+++ +   PP  + +ENV 
Sbjct: 62  EELEGFKANAWLMSPPCQPFTRLGLQKDDQDNRTNSFFHLLDVLTKIKDPPTYILIENVF 121

Query: 131 GFE---TSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRK-PLFFEKEL 190
           GF    +S+T   +++ L K  +S QEF LSP QFG+   R RYFC+AKR   L F+KE 
Sbjct: 122 GFAKKGSSNTRDHLLDTLIKMNYSFQEFHLSPQQFGLANQRLRYFCIAKRNGKLNFKKEQ 181

Query: 191 YNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELSFYDASS 250
             +      N  +D +    +   +  Q   D L        + LDH    +  F+    
Sbjct: 182 DKH------NEKVDENKLNNNSNNNNEQNKYDNL--------KILDHIPGYD--FHTTLE 241

Query: 251 IQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGIQKYFMFNRNCYSVS 301
              E++   +KD  +   E + +Y VP +L+   G    I++      NC + S
Sbjct: 242 ECDEISNYFDKDLTDD--ELYEKYKVPHNLLLSKGMLFDIKQKDSKTSNCVTKS 277

BLAST of Cucsat.G5051 vs. ExPASy Swiss-Prot
Match: O55055 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Trdmt1 PE=1 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 1.2e-36
Identity = 101/301 (33.55%), Postives = 150/301 (49.83%), Query Frame = 0

Query: 11  EPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNF-GDRPYQGNIESLT 70
           EP RVLE YSGIGGM ++L  + I A VV A D+N  AN+VY+HNF         IE ++
Sbjct: 2   EPLRVLELYSGIGGMHHALRESHIPAHVVAAIDVNTVANEVYKHNFPHTHLLSKTIEGIS 61

Query: 71  AADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRPPVMLFMENV 130
             D D    ++ L+SPPCQP+TR GLQ    D R  SFL IL+++    + P  + +ENV
Sbjct: 62  LEDFDKLSFNMILMSPPCQPFTRIGLQGDMTDPRTTSFLYILDILPRLQKLPKYILLENV 121

Query: 131 VGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPLFFEKELYNN 190
            GFE S T   +I+ +E  GF  QEF+LSP   G+P SR RYF +AK      + E +  
Sbjct: 122 KGFEVSSTRGLLIQTIEACGFQYQEFLLSPSSLGIPNSRLRYFLIAK-----LQSEPFPF 181

Query: 191 QLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELSFYDASSIQS 250
           Q   +P  +L     + ++E        +          R    S+  + S  D    + 
Sbjct: 182 Q---APGQILMEFPKIVTVEPQKYAVVEESQPRVQRTGPRICAESSSTQSSGKDTILFKL 241

Query: 251 EVALEKNK------DADEQVIEPF------NQYLVPSSLIDRWGSAMGIQKYFMFNRNCY 299
           E   E+++      D   Q+++ F      ++YL+P  L+ R+   + I K       C+
Sbjct: 242 ETVEERDRKHQQDSDLSVQMLKDFLEDGDTDEYLLPPKLLLRYALLLDIVKPTSRRSMCF 294

BLAST of Cucsat.G5051 vs. ExPASy Swiss-Prot
Match: Q4G073 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trdmt1 PE=2 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 7.9e-36
Identity = 100/303 (33.00%), Postives = 154/303 (50.83%), Query Frame = 0

Query: 11  EPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRPYQG-NIESLT 70
           EP RVLE YSGIGGM ++L  + + A VV A D++  AN+VY+HNF         IE ++
Sbjct: 2   EPLRVLELYSGIGGMHHALRESRVPAHVVAAIDVSTVANEVYKHNFPHTHLLAKTIEGIS 61

Query: 71  AADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRPPVMLFMENV 130
             + D    ++ L+SPPCQP+TR GLQ    D R  SFL IL+++    + P  + +ENV
Sbjct: 62  LEEFDKLSFNMILMSPPCQPFTRIGLQGDMSDRRTNSFLYILDILPRLQKLPKYILLENV 121

Query: 131 VGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAK--RKPLFFEKELY 190
            GFE S T   +I+ +E  GF  QEF+LSP   G+P SR RYF +AK   +PL F+    
Sbjct: 122 KGFEVSSTRGLLIQTMEACGFQYQEFLLSPSSLGIPNSRLRYFLIAKLQSEPLCFQ---- 181

Query: 191 NNQLLWSPNPLLDSDANLESIETHGSQATSD-KLLLSCEPICRFLDHSNHQ-----ELSF 250
                 +P  +L    N  +++        + KL +        LD S+ Q      + F
Sbjct: 182 ------APGQILMEFPNSGTVQPQEYAVVEEGKLRVRTREPDVCLDSSSTQCSGQDSILF 241

Query: 251 YDASSIQSEVALEKNKDADEQVIEPF------NQYLVPSSLIDRWGSAMGIQKYFMFNRN 299
              ++   +   +++ D   Q+++ F       QYL+P+  + R+   + I K       
Sbjct: 242 KHETAADIDRKRQQDSDLSVQMLKGFLEDGDTAQYLLPAKSLLRYALLLDIVKPTSRRSM 294

BLAST of Cucsat.G5051 vs. ExPASy Swiss-Prot
Match: O14717 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=TRDMT1 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.0e-35
Identity = 101/294 (34.35%), Postives = 150/294 (51.02%), Query Frame = 0

Query: 11  EPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRPYQG-NIESLT 70
           EP RVLE YSG+GGM ++L  + I A+VV A D+N  AN+VY++NF         IE +T
Sbjct: 2   EPLRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGIT 61

Query: 71  AADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRPPVMLFMENV 130
             + D     + L+SPPCQP+TR G Q    D+R  SFL IL+++    + P  + +ENV
Sbjct: 62  LEEFDRLSFDMILMSPPCQPFTRIGRQGDMTDSRTNSFLHILDILPRLQKLPKYILLENV 121

Query: 131 VGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAK--RKPLFFEKELY 190
            GFE S T   +I+ +E  GF  QEF+LSP   G+P SR RYF +AK   +PL F+    
Sbjct: 122 KGFEVSSTRDLLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYFLIAKLQSEPLPFQ---- 181

Query: 191 NNQLLWSPNPLLDSDANLESI--ETHGSQATSDKLLLSCEPICRFLDHSNHQELSFYDAS 250
                 +P  +L     +ES+  + +     +     + EP   F       + S  DA 
Sbjct: 182 ------APGQVLMEFPKIESVHPQKYAMDVENKIQEKNVEPNISF---DGSIQCSGKDAI 241

Query: 251 SIQSEVALE------KNKDADEQVIEPF-------NQYLVPSSLIDRWGSAMGI 287
             + E A E      ++ D   ++++ F       NQYL+P   + R+   + I
Sbjct: 242 LFKLETAEEIHRKNQQDSDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYALLLDI 282

BLAST of Cucsat.G5051 vs. NCBI nr
Match: KAE8652193.1 (hypothetical protein Csa_022276 [Cucumis sativus])

HSP 1 Score: 582 bits (1499), Expect = 2.37e-207
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180

Query: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240
           FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS
Sbjct: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240

Query: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMG 285
           FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMG
Sbjct: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMG 285

BLAST of Cucsat.G5051 vs. NCBI nr
Match: XP_004138735.1 (tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 580 bits (1496), Expect = 5.85e-207
Identity = 285/286 (99.65%), Postives = 285/286 (99.65%), Query Frame = 0

Query: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180

Query: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240
           FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS
Sbjct: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240

Query: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGI 286
           FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAM I
Sbjct: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMDI 286

BLAST of Cucsat.G5051 vs. NCBI nr
Match: XP_031737320.1 (tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 580 bits (1496), Expect = 1.94e-206
Identity = 285/286 (99.65%), Postives = 285/286 (99.65%), Query Frame = 0

Query: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180

Query: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240
           FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS
Sbjct: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240

Query: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGI 286
           FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAM I
Sbjct: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMDI 286

BLAST of Cucsat.G5051 vs. NCBI nr
Match: XP_008445162.1 (PREDICTED: tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 552 bits (1423), Expect = 7.64e-196
Identity = 269/286 (94.06%), Postives = 280/286 (97.90%), Query Frame = 0

Query: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELM++ASRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180
           PVMLFMENVVGFETSDTHTKMIEILEK GFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180

Query: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240
            F+K+L+NNQLLWSP+PLLD+DA+LESIETHGSQATSDKLLLSCEPICRFLDHSNH+ELS
Sbjct: 181 SFQKQLHNNQLLWSPSPLLDNDADLESIETHGSQATSDKLLLSCEPICRFLDHSNHRELS 240

Query: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGI 286
           FYDASSIQSEVALEKN+D +EQVIE FNQYLVPS LIDRWGSAM I
Sbjct: 241 FYDASSIQSEVALEKNEDPNEQVIESFNQYLVPSGLIDRWGSAMDI 286

BLAST of Cucsat.G5051 vs. NCBI nr
Match: KAA0064954.1 (tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 552 bits (1423), Expect = 1.63e-195
Identity = 269/286 (94.06%), Postives = 280/286 (97.90%), Query Frame = 0

Query: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELM++ASRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180
           PVMLFMENVVGFETSDTHTKMIEILEK GFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180

Query: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240
            F+K+L+NNQLLWSP+PLLD+DA+LESIETHGSQATSDKLLLSCEPICRFLDHSNH+ELS
Sbjct: 181 SFQKQLHNNQLLWSPSPLLDNDADLESIETHGSQATSDKLLLSCEPICRFLDHSNHRELS 240

Query: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGI 286
           FYDASSIQSEVALEKN+D +EQVIE FNQYLVPS LIDRWGSAM I
Sbjct: 241 FYDASSIQSEVALEKNEDPNEQVIESFNQYLVPSGLIDRWGSAMDI 286

BLAST of Cucsat.G5051 vs. ExPASy TrEMBL
Match: A0A0A0LSE8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380010 PE=3 SV=1)

HSP 1 Score: 580 bits (1496), Expect = 2.83e-207
Identity = 285/286 (99.65%), Postives = 285/286 (99.65%), Query Frame = 0

Query: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180

Query: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240
           FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS
Sbjct: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240

Query: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGI 286
           FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAM I
Sbjct: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMDI 286

BLAST of Cucsat.G5051 vs. ExPASy TrEMBL
Match: A0A1S3BC07 (tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488282 PE=3 SV=1)

HSP 1 Score: 552 bits (1423), Expect = 3.70e-196
Identity = 269/286 (94.06%), Postives = 280/286 (97.90%), Query Frame = 0

Query: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELM++ASRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180
           PVMLFMENVVGFETSDTHTKMIEILEK GFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180

Query: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240
            F+K+L+NNQLLWSP+PLLD+DA+LESIETHGSQATSDKLLLSCEPICRFLDHSNH+ELS
Sbjct: 181 SFQKQLHNNQLLWSPSPLLDNDADLESIETHGSQATSDKLLLSCEPICRFLDHSNHRELS 240

Query: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGI 286
           FYDASSIQSEVALEKN+D +EQVIE FNQYLVPS LIDRWGSAM I
Sbjct: 241 FYDASSIQSEVALEKNEDPNEQVIESFNQYLVPSGLIDRWGSAMDI 286

BLAST of Cucsat.G5051 vs. ExPASy TrEMBL
Match: A0A5A7VCE7 (tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002740 PE=3 SV=1)

HSP 1 Score: 552 bits (1423), Expect = 7.90e-196
Identity = 269/286 (94.06%), Postives = 280/286 (97.90%), Query Frame = 0

Query: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELM++ASRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180
           PVMLFMENVVGFETSDTHTKMIEILEK GFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180

Query: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQELS 240
            F+K+L+NNQLLWSP+PLLD+DA+LESIETHGSQATSDKLLLSCEPICRFLDHSNH+ELS
Sbjct: 181 SFQKQLHNNQLLWSPSPLLDNDADLESIETHGSQATSDKLLLSCEPICRFLDHSNHRELS 240

Query: 241 FYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGI 286
           FYDASSIQSEVALEKN+D +EQVIE FNQYLVPS LIDRWGSAM I
Sbjct: 241 FYDASSIQSEVALEKNEDPNEQVIESFNQYLVPSGLIDRWGSAMDI 286

BLAST of Cucsat.G5051 vs. ExPASy TrEMBL
Match: A0A6J1HD73 (tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111462432 PE=3 SV=1)

HSP 1 Score: 497 bits (1280), Expect = 2.22e-174
Identity = 247/287 (86.06%), Postives = 262/287 (91.29%), Query Frame = 0

Query: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKV PKLQ EPWRVLEFYSGIGGMRYSLL AGI AKV+QAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVSPKLQGEPWRVLEFYSGIGGMRYSLLRAGIDAKVIQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELM++ASRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180
           PVMLFMENVVGFETSDTHTKMIEILEK GFS QEFILSPMQF VPYSRPRYFCLAKR+PL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSRQEFILSPMQFRVPYSRPRYFCLAKRRPL 180

Query: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQ-EL 240
            F+ +L NNQLLWSP PLL++DA+L+ IE+HGSQ TSDK LLSCEPIC+FLD+SNHQ E 
Sbjct: 181 SFQTQLCNNQLLWSPWPLLENDADLDIIESHGSQTTSDKFLLSCEPICKFLDNSNHQNEF 240

Query: 241 SFYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGI 286
           S +DASSI  E ALEK KD DEQ IE F+QYLVPSSLIDRWGSAM I
Sbjct: 241 SSHDASSIHREGALEKTKDVDEQDIESFDQYLVPSSLIDRWGSAMDI 287

BLAST of Cucsat.G5051 vs. ExPASy TrEMBL
Match: A0A6J1BVV7 (DNA (Cytosine-5)-methyltransferase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005184 PE=3 SV=1)

HSP 1 Score: 496 bits (1278), Expect = 4.17e-174
Identity = 247/287 (86.06%), Postives = 261/287 (90.94%), Query Frame = 0

Query: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKV P+LQ EPWRVLEFYSGIGGMRYSLLSAG+ AKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQK SGDARAFSFLKILELM++ SRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180
           PVMLFMENVVGFETSDTHTKMIEILEK  FS QEFILSPMQFGVPYSRPRYFCLAKR+PL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180

Query: 181 FFEKELYNNQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNHQ-EL 240
            F + LYNNQLLWSP+PLL++D ++E+IE HGSQATSDKLLLSCEPICRFLDHS+H  E 
Sbjct: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240

Query: 241 SFYDASSIQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGI 286
           SF DASSIQ E ALEK KD DEQ    F+QYLVPSSLIDRWGSAM I
Sbjct: 241 SFSDASSIQREGALEKTKDTDEQ--GSFDQYLVPSSLIDRWGSAMDI 285

BLAST of Cucsat.G5051 vs. TAIR 10
Match: AT5G25480.1 (DNA methyltransferase-2 )

HSP 1 Score: 340.1 bits (871), Expect = 1.9e-93
Identity = 172/280 (61.43%), Postives = 212/280 (75.71%), Query Frame = 0

Query: 9   QDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRPYQGNIESL 68
           + +PW+VLEFYSGIGGMRYSL+++GI ++VV+AF+IND ANDVY+HNF  RPYQGNI+SL
Sbjct: 11  EKKPWQVLEFYSGIGGMRYSLMASGIVSEVVEAFEINDSANDVYQHNFKHRPYQGNIQSL 70

Query: 69  TAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMVEASRPPVMLFMEN 128
           TAAD+D Y A  WLLSPPCQPYTRQGLQK SGDARAFSFL+ILEL+   ++PP MLF+EN
Sbjct: 71  TAADLDKYNADAWLLSPPCQPYTRQGLQKHSGDARAFSFLRILELIPHTTKPPQMLFVEN 130

Query: 129 VVGFETSDTHTKMIEILEKTGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPLFFEKELYN 188
           VVGFETSDTH +MI  L K  + TQEFILSP+QFGVPYSRPRYFCLAKRKPL F+ +  N
Sbjct: 131 VVGFETSDTHMEMIGTLTKLDYVTQEFILSPLQFGVPYSRPRYFCLAKRKPLPFKSQHSN 190

Query: 189 NQLLWSPNPLLDSDANLESIETHGSQATSDKLLLSCEPICRFLDHSNH--QELSFYDASS 248
           N+LLWSP+PL   D  +E  +    +   DKLL  C+P+ +FL+ + H   E S  D S 
Sbjct: 191 NKLLWSPDPLYGRDDQVEFGKCQAEEGL-DKLLEFCKPVEKFLELAAHVDGEPSSVDDSE 250

Query: 249 IQSEVALEKNKDADEQVIEPFNQYLVPSSLIDRWGSAMGI 287
             S+    +  D+   V +  +QYLVP SLI+RWG+AM I
Sbjct: 251 NGSKDCCGQEGDS---VPDSVHQYLVPVSLIERWGNAMDI 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JWT72.7e-9261.43tRNA (cytosine(38)-C(5))-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q54JH62.1e-4135.71DNA (cytosine-5)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=dnmA ... [more]
O550551.2e-3633.55tRNA (cytosine(38)-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Trdmt1 PE... [more]
Q4G0737.9e-3633.00tRNA (cytosine(38)-C(5))-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trdm... [more]
O147173.0e-3534.35tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=TRDMT1 PE=... [more]
Match NameE-valueIdentityDescription
KAE8652193.12.37e-207100.00hypothetical protein Csa_022276 [Cucumis sativus][more]
XP_004138735.15.85e-20799.65tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X2 [Cucumis sativus][more]
XP_031737320.11.94e-20699.65tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X1 [Cucumis sativus][more]
XP_008445162.17.64e-19694.06PREDICTED: tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo][more]
KAA0064954.11.63e-19594.06tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A0A0LSE82.83e-20799.65Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380010 PE=3 SV=1[more]
A0A1S3BC073.70e-19694.06tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN... [more]
A0A5A7VCE77.90e-19694.06tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo var. makuw... [more]
A0A6J1HD732.22e-17486.06tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1BVV74.17e-17486.06DNA (Cytosine-5)-methyltransferase isoform X1 OS=Momordica charantia OX=3673 GN=... [more]
Match NameE-valueIdentityDescription
AT5G25480.11.9e-9361.43DNA methyltransferase-2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.120.10DNA Methylase, subunit A, domain 2coord: 132..255
e-value: 6.2E-39
score: 135.0
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 3..81
e-value: 1.2E-23
score: 85.9
NoneNo IPR availablePANTHERPTHR46098TRNA (CYTOSINE(38)-C(5))-METHYLTRANSFERASEcoord: 4..270
IPR001525C-5 cytosine methyltransferasePFAMPF00145DNA_methylasecoord: 7..265
e-value: 4.4E-19
score: 69.0
IPR001525C-5 cytosine methyltransferasePROSITEPS51679SAM_MT_C5coord: 1..272
score: 21.006077
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 6..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G5051.T2Cucsat.G5051.T2mRNA
Cucsat.G5051.T1Cucsat.G5051.T1mRNA
Cucsat.G5051.T12Cucsat.G5051.T12mRNA
Cucsat.G5051.T13Cucsat.G5051.T13mRNA
Cucsat.G5051.T14Cucsat.G5051.T14mRNA
Cucsat.G5051.T6Cucsat.G5051.T6mRNA
Cucsat.G5051.T3Cucsat.G5051.T3mRNA
Cucsat.G5051.T4Cucsat.G5051.T4mRNA
Cucsat.G5051.T17Cucsat.G5051.T17mRNA
Cucsat.G5051.T9Cucsat.G5051.T9mRNA
Cucsat.G5051.T18Cucsat.G5051.T18mRNA
Cucsat.G5051.T19Cucsat.G5051.T19mRNA
Cucsat.G5051.T10Cucsat.G5051.T10mRNA
Cucsat.G5051.T16Cucsat.G5051.T16mRNA
Cucsat.G5051.T20Cucsat.G5051.T20mRNA
Cucsat.G5051.T15Cucsat.G5051.T15mRNA
Cucsat.G5051.T5Cucsat.G5051.T5mRNA
Cucsat.G5051.T11Cucsat.G5051.T11mRNA
Cucsat.G5051.T8Cucsat.G5051.T8mRNA
Cucsat.G5051.T7Cucsat.G5051.T7mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity