Cucsat.G4968 (gene) Cucumber (B10) v3

Overview
NameCucsat.G4968
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionMultifunctional methyltransferase subunit TRM112-like protein
Locationctg1227: 1058551 .. 1061073 (-)
RNA-Seq ExpressionCucsat.G4968
SyntenyCucsat.G4968
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TACTGAATGGAGAGAGTGAGAAATAAGAAAAAAAATAAAATAGAATAGTAGTAATCGTAGATTGTTGGAGCAAATCTGAAATTCTACTCCACGGTAATCGGAGGGCCAAACGGCTCCTAAGTGATTAGAAAAGAAACAACGTCTAAAAAGCTAAAACTTGGGTTTGAGATTGAGCAATGGAAATTAGGGTTTTACTTAAGTAAAAGAAATAAAAATAAATGAAAACAAGGCTTCGGTTTTATCAGAATTAGCCGCCAGACTCCCCATCTTCCATTCTTCTCCGTGAGCGCCAGCGGCAACGATCACACACTCTCTCTTCACTTCACCAGCACCCTCCACACCACGCTGCCGACTTCCCTCCGCCGTCCTCCACGGTAAACCGCCGCCCTTTTTCTTTTCTTGCCTCTCTCTCTCTATCGGCCTTCTCATGTTGTTCTCTGCAGGTCGTGACCACGGAGCACAACCGCCGTTTAAGCCGTCGCCTCCGTTTTTCGTCAGAACCGTCTGCCAGCATTGTTTGAGGTGAATTTCCGGCACTTTTCTGTGGGTATTTTCAGTGTGATTTTGATTTTCGTGGCTTGGTTTGGCTTAAAATAAACTACCTTTCTAGTAGGTTAGGGGTTTGGCGGGACTATATCTGCCGTCTAATTCGTTAAAATCTTCTTGTAGTTGCTGGAATTTGGGTTTATCAAACTATAATTGCCATTTTTTTTGCTTAGGTCTAGTTATCTTCTATGGGATCAGGAGTATTGGATCAAGAGATTCTTTGGATCAGGAGTATTGGATCAATTGCCAATTTAAATGGTTTAGGGTTGGTATTGATTTAAGGCTATTAAGTTAATAATTGGGTTTGAAGGCGTCGGGTTGATATGCCAATTTTAGATAAAGAGGAGAGTTGAGAAAGAGAAACTATTTGCTAGTTGTTTTTTTTTTAAGGTCTCGGCGTTAGATGAATTTAATTGTGAACTTCTTGAGGCCATTTGTTCTTTCTTTGATGTATGGTTGCATCTTTAAGTTCGAACTAGCATTGAACATTGGGTAACATGTTTAGACTTAGCATATGGGTCATCAAACTTGCTAAGTAATTGAGTCTTCTTGGAAGACTGCTAAATTCTTTGGACTATTCTTTGAATTTAGCATTAGTTATTTAATCCTTGTTTTGGTTGGACTTTTGAAGAAATCTTTAAAATACTCTTTTAAATTGGGTTATGGTTTAAGCTATCGTCTTGGTTCTTTATATTTTAGGTCGTGATCTGGGATATTATGTTTTCAATTACAGGTTGATTGAACTTCTATCTTGTAGAAGTCCTTAATAGGAACCTAGAATTGTTGTTTCTTAGGTGTTAAGTAGATGGGTATTTTTGCAGCATACCCCAAGCCGAAAATTTGGGAATGTTAAATTAAAAATATGGTCTATTTTCTCTTTTGTCACTCTTGCACTGTTCTAGATCGTAATTTTGTTCAACATTCTAATAGATTTTTAACAGTTGTGAAAGATTTATGAAATCTCTAAGTATATATGGAGTTCTTGAAATTATAGACCCATCTTTTAAGCTAAAGGCGAAGTTCAAGAGTTAGTTATTCTTTTGTAACCAAATCCAATCATGCAGATACAAACACACAATTAAATGTATAAATAACAAGCAAGTCTAGTACCTTTAACTTTTCTTATAAAGCCCTAGGTTGCTCTCAAAAGCAGGATTTTGGTATTTGGTATTGACTCTCTTGTCGTGACACTGAATACCTATTACCAAGCAGGTAACTTGAAGAGGAGAAGATGAGGCTACTGACTCATAACATGCTCTCATCTAACATCAAGGGTGTCATCAATGGCTTCCCTCTTCGAATTGAGGTCGAGAAAGTTGTTGAGAAGCCAGTTGACTTCAATCCAGACTTCCTCAAGAACATGTTCTCCAAGATTGAATGGAAACCCCTTGTCGATGCCTCTCGGACTATGGGCTATGCCGAGCTTCCCGAGGAGGCCGATGCATCAATGCTAGACTCCCATGACTTTCTCCAACGGTTTCACCATGCACTTCTGGAGTTGCATCTTGAGGAAGGCGCTCTTTTTTGCCCAGAGACCAACAGAAGATTCCCCGTCAATAAAGGCATTCCCAATATGCTTCTTCACGAAGACGAGGTCTGAGTTTGAGATTTTACAAAATCCTCTATTTTCTCCTATATCTAATTCAATGCTGAAAAGGAAAATCTTGGGTTTCACTAGACGAAGCACAGGGTATTTGTGAACGCTTTGTCATGAGTTTGGAAATTAGGGTTTACGCCTTTCTTTGCTTTTGTTAGCTGTGAATGAGATCATTATTAGATCTTTGTTTACTGATTTGATGGGTGTAAACTGCTTCGGAACATATGATTTCTATATGAATTTTAGTGCTATGCTTAAAGTAAGAAATGTGCAAAATGGAAAATAGAGGAGCCAAATATTCAATATTTTCACAATTTCACTACATAGATTTCGTAATTTCAAATTTACAGCTTCAAGGCTACCAATTTTTTAGATTTTTA

Coding sequence (CDS)

ATGAGGCTACTGACTCATAACATGCTCTCATCTAACATCAAGGGTGTCATCAATGGCTTCCCTCTTCGAATTGAGGTCGAGAAAGTTGTTGAGAAGCCAGTTGACTTCAATCCAGACTTCCTCAAGAACATGTTCTCCAAGATTGAATGGAAACCCCTTGTCGATGCCTCTCGGACTATGGGCTATGCCGAGCTTCCCGAGGAGGCCGATGCATCAATGCTAGACTCCCATGACTTTCTCCAACGGTTTCACCATGCACTTCTGGAGTTGCATCTTGAGGAAGGCGCTCTTTTTTGCCCAGAGACCAACAGAAGATTCCCCGTCAATAAAGGCATTCCCAATATGCTTCTTCACGAAGACGAGGTCTGA

Protein sequence

MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTMGYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHEDEV
Homology
BLAST of Cucsat.G4968 vs. ExPASy Swiss-Prot
Match: Q8LFJ5 (Multifunctional methyltransferase subunit TRM112 homolog A OS=Arabidopsis thaliana OX=3702 GN=TRM112A PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 4.5e-48
Identity = 92/124 (74.19%), Postives = 109/124 (87.90%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRL+THNMLS NIKGV +GFPLRIE   V+EK VDFNPDF+++MF+KIEWK LV+ +R+M
Sbjct: 1   MRLITHNMLSCNIKGVTSGFPLRIEAGNVIEKEVDFNPDFIRHMFAKIEWKALVEGARSM 60

Query: 61  GYAELPEEA-DASMLDSHD-FLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLH 120
           GYAELPEE+ DA++L S + FL++ HHALLELHLEEGAL CPET R+FPVNKGIPNMLLH
Sbjct: 61  GYAELPEESPDAAVLKSDEPFLKKLHHALLELHLEEGALVCPETGRKFPVNKGIPNMLLH 120

Query: 121 EDEV 123
           EDEV
Sbjct: 121 EDEV 124

BLAST of Cucsat.G4968 vs. ExPASy Swiss-Prot
Match: Q9C9R3 (Multifunctional methyltransferase subunit TRM112 homolog B OS=Arabidopsis thaliana OX=3702 GN=TRM112B PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 2.3e-44
Identity = 87/124 (70.16%), Postives = 103/124 (83.06%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRL+ HNMLS NIKGV+N FPLRIE EKV  K VDFNPDFL+ MF+KI+WK LVD +R+M
Sbjct: 1   MRLIVHNMLSCNIKGVVNKFPLRIEAEKVTVKEVDFNPDFLRYMFAKIDWKALVDGARSM 60

Query: 61  GYAELPEEA-DASMLDSHD-FLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLH 120
            Y ELP+ A D + L+S + FL++FHHALLELHLEEG+L CPET R+F V+KGIPNMLLH
Sbjct: 61  EYTELPDNAPDTTTLESDETFLRKFHHALLELHLEEGSLVCPETGRKFSVSKGIPNMLLH 120

Query: 121 EDEV 123
           EDEV
Sbjct: 121 EDEV 124

BLAST of Cucsat.G4968 vs. ExPASy Swiss-Prot
Match: Q9DCG9 (Multifunctional methyltransferase subunit TRM112-like protein OS=Mus musculus OX=10090 GN=Trmt112 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 2.7e-29
Identity = 59/122 (48.36%), Postives = 86/122 (70.49%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGV-INGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRT 60
           M+LLTHN+LSS+++GV   GFPLR++  +V   PV+FNP+F+  M  K+EW  LV A+ T
Sbjct: 1   MKLLTHNLLSSHVRGVGTRGFPLRLQATEVRINPVEFNPEFVARMIPKVEWAALVQAADT 60

Query: 61  MGYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHE 120
           +  AE+P+E          FL++ HH LLE+ + EG L CPE+ R FP+++GIPNMLL++
Sbjct: 61  LNLAEVPKEPTEGYEHDETFLRKMHHVLLEVDVLEGTLQCPESGRLFPISRGIPNMLLND 120

Query: 121 DE 122
           +E
Sbjct: 121 EE 122

BLAST of Cucsat.G4968 vs. ExPASy Swiss-Prot
Match: Q2KIA2 (Multifunctional methyltransferase subunit TRM112-like protein OS=Bos taurus OX=9913 GN=TRMT112 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 8.8e-28
Identity = 57/122 (46.72%), Postives = 86/122 (70.49%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGV-INGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRT 60
           MRLLTHN+LSS+++GV   GFPLR++  +V   PV+FNPDF+  M  K+EW  L++A+  
Sbjct: 1   MRLLTHNLLSSHVRGVGPRGFPLRLQATEVRINPVEFNPDFIVRMIPKVEWAALLEAADH 60

Query: 61  MGYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHE 120
           +   ++P+E       + +FL++ HH LLE+ + EG L CPE+ R FP+++GIPNMLL +
Sbjct: 61  LHLIQVPKEPIQGYEHNEEFLRKMHHVLLEVEVLEGTLQCPESGRVFPISRGIPNMLLSD 120

Query: 121 DE 122
           +E
Sbjct: 121 EE 122

BLAST of Cucsat.G4968 vs. ExPASy Swiss-Prot
Match: Q9UI30 (Multifunctional methyltransferase subunit TRM112-like protein OS=Homo sapiens OX=9606 GN=TRMT112 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 3.7e-26
Identity = 54/122 (44.26%), Postives = 84/122 (68.85%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGV-INGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRT 60
           M+LLTHN+LSS+++GV   GFPLR++  +V   PV+FNP+F+  M  K+EW   ++A+  
Sbjct: 1   MKLLTHNLLSSHVRGVGSRGFPLRLQATEVRICPVEFNPNFVARMIPKVEWSAFLEAADN 60

Query: 61  MGYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHE 120
           +   ++P+       ++ +FL+  HH LLE+ + EG L CPE+ R FP+++GIPNMLL E
Sbjct: 61  LRLIQVPKGPVEGYEENEEFLRTMHHLLLEVEVIEGTLQCPESGRMFPISRGIPNMLLSE 120

Query: 121 DE 122
           +E
Sbjct: 121 EE 122

BLAST of Cucsat.G4968 vs. NCBI nr
Match: XP_004152640.1 (multifunctional methyltransferase subunit TRM112 homolog A [Cucumis sativus] >XP_031736795.1 multifunctional methyltransferase subunit TRM112 homolog A [Cucumis sativus] >KGN62692.1 hypothetical protein Csa_022115 [Cucumis sativus])

HSP 1 Score: 252 bits (643), Expect = 1.85e-84
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM
Sbjct: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60

Query: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120
           GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED
Sbjct: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120

Query: 121 EV 122
           EV
Sbjct: 121 EV 122

BLAST of Cucsat.G4968 vs. NCBI nr
Match: XP_008444834.1 (PREDICTED: multifunctional methyltransferase subunit TRM112-like protein At1g22270 [Cucumis melo] >XP_008444835.1 PREDICTED: multifunctional methyltransferase subunit TRM112-like protein At1g22270 [Cucumis melo])

HSP 1 Score: 249 bits (637), Expect = 1.52e-83
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM
Sbjct: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60

Query: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120
           GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGAL CPETNRRFPVNKGIPNMLLHED
Sbjct: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALICPETNRRFPVNKGIPNMLLHED 120

Query: 121 EV 122
           EV
Sbjct: 121 EV 122

BLAST of Cucsat.G4968 vs. NCBI nr
Match: KAA0065150.1 (multifunctional methyltransferase subunit TRM112-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 249 bits (637), Expect = 1.40e-82
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM
Sbjct: 67  MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 126

Query: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120
           GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGAL CPETNRRFPVNKGIPNMLLHED
Sbjct: 127 GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALICPETNRRFPVNKGIPNMLLHED 186

Query: 121 EV 122
           EV
Sbjct: 187 EV 188

BLAST of Cucsat.G4968 vs. NCBI nr
Match: XP_038885364.1 (multifunctional methyltransferase subunit TRM112 homolog A-like [Benincasa hispida] >XP_038885365.1 multifunctional methyltransferase subunit TRM112 homolog A-like [Benincasa hispida] >XP_038885366.1 multifunctional methyltransferase subunit TRM112 homolog A-like [Benincasa hispida])

HSP 1 Score: 244 bits (623), Expect = 2.08e-81
Identity = 117/122 (95.90%), Postives = 120/122 (98.36%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRLLTHNMLSSNIKG+INGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM
Sbjct: 1   MRLLTHNMLSSNIKGIINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60

Query: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120
           GYAELPEEA+ASMLDSHDFLQRFHHALLELHLEEGAL CPET RRFPVNKGIPN+LLHED
Sbjct: 61  GYAELPEEAEASMLDSHDFLQRFHHALLELHLEEGALICPETGRRFPVNKGIPNLLLHED 120

Query: 121 EV 122
           EV
Sbjct: 121 EV 122

BLAST of Cucsat.G4968 vs. NCBI nr
Match: XP_023548932.1 (multifunctional methyltransferase subunit TRM112-like protein At1g22270 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 240 bits (612), Expect = 9.89e-80
Identity = 115/122 (94.26%), Postives = 119/122 (97.54%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRLLTHNMLSSNIKGV+NGFPLRIEVEKVVEK VDFNPDFLKNMFSKIEWKPLVDASRTM
Sbjct: 1   MRLLTHNMLSSNIKGVVNGFPLRIEVEKVVEKQVDFNPDFLKNMFSKIEWKPLVDASRTM 60

Query: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120
           GYAELPEEA++SMLDSHDFLQRFHHALLELHLEEGAL CPET RRFPVNKGIPN+LLHED
Sbjct: 61  GYAELPEEAESSMLDSHDFLQRFHHALLELHLEEGALICPETGRRFPVNKGIPNLLLHED 120

Query: 121 EV 122
           EV
Sbjct: 121 EV 122

BLAST of Cucsat.G4968 vs. ExPASy TrEMBL
Match: A0A0A0LPH5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G368930 PE=3 SV=1)

HSP 1 Score: 252 bits (643), Expect = 8.93e-85
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM
Sbjct: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60

Query: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120
           GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED
Sbjct: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120

Query: 121 EV 122
           EV
Sbjct: 121 EV 122

BLAST of Cucsat.G4968 vs. ExPASy TrEMBL
Match: A0A1S3BBA0 (multifunctional methyltransferase subunit TRM112-like protein At1g22270 OS=Cucumis melo OX=3656 GN=LOC103488061 PE=3 SV=1)

HSP 1 Score: 249 bits (637), Expect = 7.35e-84
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM
Sbjct: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60

Query: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120
           GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGAL CPETNRRFPVNKGIPNMLLHED
Sbjct: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALICPETNRRFPVNKGIPNMLLHED 120

Query: 121 EV 122
           EV
Sbjct: 121 EV 122

BLAST of Cucsat.G4968 vs. ExPASy TrEMBL
Match: A0A5A7VD78 (Multifunctional methyltransferase subunit TRM112-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004860 PE=3 SV=1)

HSP 1 Score: 249 bits (637), Expect = 6.76e-83
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM
Sbjct: 67  MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 126

Query: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120
           GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGAL CPETNRRFPVNKGIPNMLLHED
Sbjct: 127 GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALICPETNRRFPVNKGIPNMLLHED 186

Query: 121 EV 122
           EV
Sbjct: 187 EV 188

BLAST of Cucsat.G4968 vs. ExPASy TrEMBL
Match: A0A6J1JT67 (multifunctional methyltransferase subunit TRM112-like protein At1g22270 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488115 PE=3 SV=1)

HSP 1 Score: 238 bits (606), Expect = 3.94e-79
Identity = 114/122 (93.44%), Postives = 118/122 (96.72%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRLLTHNMLSSNIKGV+NGFPLRIEVEKVVEK VDFNPDFLKNMFSKIEWKPLVDASRTM
Sbjct: 1   MRLLTHNMLSSNIKGVVNGFPLRIEVEKVVEKQVDFNPDFLKNMFSKIEWKPLVDASRTM 60

Query: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120
           GYAELPEEA++S LDSHDFLQRFHHALLELHLEEGAL CPET RRFPVNKGIPN+LLHED
Sbjct: 61  GYAELPEEAESSKLDSHDFLQRFHHALLELHLEEGALICPETGRRFPVNKGIPNLLLHED 120

Query: 121 EV 122
           EV
Sbjct: 121 EV 122

BLAST of Cucsat.G4968 vs. ExPASy TrEMBL
Match: A0A6J1JUC5 (multifunctional methyltransferase subunit TRM112-like protein At1g22270 OS=Cucurbita maxima OX=3661 GN=LOC111487970 PE=3 SV=1)

HSP 1 Score: 237 bits (605), Expect = 5.60e-79
Identity = 114/122 (93.44%), Postives = 118/122 (96.72%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRLLTHNMLSSNIKGV+NGFPLRIEVEKVVEK VDFNPDFLKNMFSKIEWKPLVDASR M
Sbjct: 1   MRLLTHNMLSSNIKGVVNGFPLRIEVEKVVEKQVDFNPDFLKNMFSKIEWKPLVDASRAM 60

Query: 61  GYAELPEEADASMLDSHDFLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLHED 120
           GYAELPEEA++SMLDSHDFLQRFHHALLELHLEEGAL CPET RRFPVNKGIPN+LLHED
Sbjct: 61  GYAELPEEAESSMLDSHDFLQRFHHALLELHLEEGALICPETARRFPVNKGIPNLLLHED 120

Query: 121 EV 122
           EV
Sbjct: 121 EV 122

BLAST of Cucsat.G4968 vs. TAIR 10
Match: AT1G22270.1 (Trm112p-like protein )

HSP 1 Score: 191.8 bits (486), Expect = 3.2e-49
Identity = 92/124 (74.19%), Postives = 109/124 (87.90%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRL+THNMLS NIKGV +GFPLRIE   V+EK VDFNPDF+++MF+KIEWK LV+ +R+M
Sbjct: 1   MRLITHNMLSCNIKGVTSGFPLRIEAGNVIEKEVDFNPDFIRHMFAKIEWKALVEGARSM 60

Query: 61  GYAELPEEA-DASMLDSHD-FLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLH 120
           GYAELPEE+ DA++L S + FL++ HHALLELHLEEGAL CPET R+FPVNKGIPNMLLH
Sbjct: 61  GYAELPEESPDAAVLKSDEPFLKKLHHALLELHLEEGALVCPETGRKFPVNKGIPNMLLH 120

Query: 121 EDEV 123
           EDEV
Sbjct: 121 EDEV 124

BLAST of Cucsat.G4968 vs. TAIR 10
Match: AT1G78190.1 (Trm112p-like protein )

HSP 1 Score: 179.5 bits (454), Expect = 1.6e-45
Identity = 87/124 (70.16%), Postives = 103/124 (83.06%), Query Frame = 0

Query: 1   MRLLTHNMLSSNIKGVINGFPLRIEVEKVVEKPVDFNPDFLKNMFSKIEWKPLVDASRTM 60
           MRL+ HNMLS NIKGV+N FPLRIE EKV  K VDFNPDFL+ MF+KI+WK LVD +R+M
Sbjct: 1   MRLIVHNMLSCNIKGVVNKFPLRIEAEKVTVKEVDFNPDFLRYMFAKIDWKALVDGARSM 60

Query: 61  GYAELPEEA-DASMLDSHD-FLQRFHHALLELHLEEGALFCPETNRRFPVNKGIPNMLLH 120
            Y ELP+ A D + L+S + FL++FHHALLELHLEEG+L CPET R+F V+KGIPNMLLH
Sbjct: 61  EYTELPDNAPDTTTLESDETFLRKFHHALLELHLEEGSLVCPETGRKFSVSKGIPNMLLH 120

Query: 121 EDEV 123
           EDEV
Sbjct: 121 EDEV 124

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LFJ54.5e-4874.19Multifunctional methyltransferase subunit TRM112 homolog A OS=Arabidopsis thalia... [more]
Q9C9R32.3e-4470.16Multifunctional methyltransferase subunit TRM112 homolog B OS=Arabidopsis thalia... [more]
Q9DCG92.7e-2948.36Multifunctional methyltransferase subunit TRM112-like protein OS=Mus musculus OX... [more]
Q2KIA28.8e-2846.72Multifunctional methyltransferase subunit TRM112-like protein OS=Bos taurus OX=9... [more]
Q9UI303.7e-2644.26Multifunctional methyltransferase subunit TRM112-like protein OS=Homo sapiens OX... [more]
Match NameE-valueIdentityDescription
XP_004152640.11.85e-84100.00multifunctional methyltransferase subunit TRM112 homolog A [Cucumis sativus] >XP... [more]
XP_008444834.11.52e-8399.18PREDICTED: multifunctional methyltransferase subunit TRM112-like protein At1g222... [more]
KAA0065150.11.40e-8299.18multifunctional methyltransferase subunit TRM112-like protein [Cucumis melo var.... [more]
XP_038885364.12.08e-8195.90multifunctional methyltransferase subunit TRM112 homolog A-like [Benincasa hispi... [more]
XP_023548932.19.89e-8094.26multifunctional methyltransferase subunit TRM112-like protein At1g22270 [Cucurbi... [more]
Match NameE-valueIdentityDescription
A0A0A0LPH58.93e-85100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G368930 PE=3 SV=1[more]
A0A1S3BBA07.35e-8499.18multifunctional methyltransferase subunit TRM112-like protein At1g22270 OS=Cucum... [more]
A0A5A7VD786.76e-8399.18Multifunctional methyltransferase subunit TRM112-like protein OS=Cucumis melo va... [more]
A0A6J1JT673.94e-7993.44multifunctional methyltransferase subunit TRM112-like protein At1g22270 isoform ... [more]
A0A6J1JUC55.60e-7993.44multifunctional methyltransferase subunit TRM112-like protein At1g22270 OS=Cucur... [more]
Match NameE-valueIdentityDescription
AT1G22270.13.2e-4974.19Trm112p-like protein [more]
AT1G78190.11.6e-4570.16Trm112p-like protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 115..122
NoneNo IPR availableGENE3D2.20.25.10coord: 1..122
e-value: 8.0E-40
score: 138.0
NoneNo IPR availablePANTHERPTHR12773:SF5BNAA09G30730D PROTEINcoord: 1..122
NoneNo IPR availableSUPERFAMILY158997Trm112p-likecoord: 87..121
IPR005651Trm112-likePFAMPF03966Trm112pcoord: 3..111
e-value: 4.4E-14
score: 52.8
IPR039127Multifunctional methyltransferase subunit Trm112PANTHERPTHR12773UPF0315 PROTEIN-RELATEDcoord: 1..122

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G4968.T1Cucsat.G4968.T1mRNA
Cucsat.G4968.T3Cucsat.G4968.T3mRNA
Cucsat.G4968.T4Cucsat.G4968.T4mRNA
Cucsat.G4968.T2Cucsat.G4968.T2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018364 peptidyl-glutamine methylation
biological_process GO:0070476 rRNA (guanine-N7)-methylation
biological_process GO:0030488 tRNA methylation
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0046982 protein heterodimerization activity