Cucsat.G4825 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G4825
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Chloride channel protein
Location	ctg1227: 4265014 .. 4268719 (+)
RNA-Seq Expression	Cucsat.G4825
Synteny	Cucsat.G4825

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGGTGTCACAATTGTAAATTAATGATCCAAATGGGCTCAAACCAAATGAGTGTTAAAGTTGTTTTCTAATACATCACGACGAAGGAGTAAGGGTAGAGATTGAACGTCTGACCTTTAAAAAGAGAATACATGTTGATACCAAGATAAGTTGGAGTCTTGATGAAAACAAATAAACATGATTTTTTTTTTCCACCCAAAAAGAACTACTGACAAACCAAAAATGAAAAGTGGAAAAAGGGTAAAGAATATGGGAAAAAATGAACTGGCCATTAAAATGACCACAAGGGAGAAAATGACAAGACAAGTGCATGATTGAGACGACATTGTTGCATTAAAATCCTAGAACAGAGGAGAAGCTTTGCACTTTCTCCCAATCCACAAACTAAACCCAACCCCAATACTTGACCATCTTCTCTTCTCGTCTCTCTGTGTCCATTTCACTGCACCAATGGATGAGAATCCAAGCTTTGTTACAGAATCAACTCTCACAAACTCCATGGAAGCAGAACCTCAAGATGAAGAAAGAGACCCAGAAAGCAATCCTTTAAACCAACCCCTTCTCAAGAGAAACAGAACTCTCTCTTCTAGTCCTCTTGCCATTGTTGGGGCAAAAGTTTCTCTCATTGAAAGCTTGGATTACGAGTAAGAATAGTACTACATGAAAGCAAAAGCATACCTTTTTTTTTTTCCTTTTTCGTTGAATTCTCCAATTTGTTGAATTGGATTTGGTTTGAATTCAGGATCAATGAGAACGATCTGTTTAAGCATGATTGGAGAAGCAGATCCAAAGTTCAAGTGCTGCAGTACATATTCTCTAAATGGACTTTGGCTTGTCTTGTTGGACTTCTTACAGGGATCATTGCCACTCTCATCAATCTTGCCATTGAAAATATTGCTGGCTACAAGCTTTTGAAAGTGGTTGATTACATAAAGGAAGAAAGGTAAGTACATACGAACCAATTAGACTTGGTTTTGTTCGCCTGCAAAAACAGAAACTTAATGAATTAAAAAATTTATGGTTTCCAAATGATTTGGATTAAGGTACTTAATGGGGTTCGCATACTTTACCACTGCCAACTTCCTTCTGACTTTTGTTGCTGCTGCTCTCTGTGTATGCTTTGCTCCCACCGCAGCTGGGCCTGGCATACCAGAAATTAAAGCCTATCTCAATGGCATAGACACTCCTAATATGTTCGGCGCCACGACGTTGATCGTTAAGGTCTGTATGCCACATACTTTCTTGTTGAATCTCAGCACAACGAACACGGTCGATCGAGTCATCTTTGGATTGTCATCATCACTGTATATTGCATATCAGCATTTGCTTTTGATTGATTGATTGAATAGGTTTAAGATTTAGAAAGGTTACGTAATGCCATGAATGCCAATGCCATTCATTGATCAATCTACAAAATGAATCAGACTGATCTGCAGCTCATACAGAATGTAGATAATGAAATTACTCCTTTGATCTTTGATATCTATAAGGTTTGATCCTTGTAGATTGTTGGAAGCATTGGAGCAGTGGCAGCTGGGCTAGATCTTGGGAAAGAAGGGCCTCTAGTACACATTGGCAGCTGCATTGCTTCTTTACTTGGACAGGGTGGACCAGATAACTATCGAGTCAAATGGACATGGCTACGCTATTTCAACAATGATCGTGATCGTCGGGATCTTATCACATGCGGGGCATCATCAGGGGTTTGTGCAGCTTTTCGGGCTCCAGTTGGGGGTGTCCTATTTGCTCTAGAAGAGGTGGCTACTTGGTGGAGGAGTGCCCTGCTATGGAGGACCTTCTTCAGCACAGCCATTGTTGTGGTAGTGCTAAGAACTTTCATTGAAATATGCAAAGCAGGTGATTGTGGGCTGTTTGGAGAAGGAGGGCTCATCATGTTTGATGTCAGTGGTGTTTCTGTAAGTTACCATATCATGGACATTATTCCAGTTGCTATCATTGGCCTTCTTGGTGGATTCCTTGGAAGCCTTTACAATCATCTTCTCCACAAAATCCTCAGAGTTTACAACCTCATCAATCAGTAAGCAAATTCACTCAAAAGGAAACTAGATTCAAATATTTGTCATAATTAGACTAACTTGCAGAATGATTTGATGAGAATTGCAATGGTGAAAATGGTATTTGCAGGAAAGGGAGAATGCACAAACTATTACTAGCCTTAGCAGTATCACTTTTCACTTCAATTTGCCAATACAGTTTGCCATACCTTGTTCAATGCACTCCCTGCAACTCTTCTCTTTCAGATTCAGCTTGCCCAACCAATGGCCGGTCCGGCAACTTCAAGCAATTCAACTGTCCTAAAGGCTATTACAATGATCTCGCTACCCTTCTTCTCACCACCAACGACGACGCTGTTCGAAACATCTTCTCCATCAACACTCCAGCCGAGTATCAGCCTCTTTCCCTTGTCATCTTCTTCCTTCTCTACTGCATATTAGGGTTATTCACCTTCGGTATCGCCGTCCCTTCCGGTCTCTTCCTTCCGATCATCTTGATGGGCTCTGGCTATGGCCGCCTCATCGGCCTCCTCATGCGACCCTACACCAATCTCGATCAAGGACTCTTAGCTGTTCTCGGTGCTGCTTCCTTAATGGCCGGCTCCATGCGAATGACCGTTTCTCTTTGTGTCATATTCCTCGAACTCACCAACAATCTCCTTCTCCTTCCGATCACCATGATTGTTCTTCTAATTGCAAAAACAGTCGGAGACAGTTTTAATCCTAGCATTTACGACATCATTCTCCACCTCAAAGGACTTCCATTCCTCGACGCGAATCCGGAGCCATGGATGAGAAACATAACCGTCGGTGAGCTCGCCGACGCGAAACCAGCGGTGGTGACACTTCGGGGATTAGAGAAAGTATCACGCATTGTGGAGGTTCTTAGGAACACAACACACAATGGATTCCCTGTTGTGGATGCCGATGCGGTTGTGCCGCCGGTGGGCATGGCAGTGGGAGCGACGGAGCTGCACGGACTAGTACTGAGAGCGCATCTACTACAAGTGCTGAAGAAGAAATGGTTCTTGAGAGAGAGGAGGAGGACGGAGGACTGGGAAGTGAGGGAGAAATTTACATGGGTGGAATTGGCGGAGAGGGAAGGAAAGATCGAAGAGCTTGTAGTGACGAAAGAGGAAATGGAAATGTACGTTGATCTTCATCCATTGACGAACACTACACCGTATACAGTACTGGAAAGCATGTCGGTGGCAAAAGCTTTGGTTCTTTTCCGGCAAGTAGGACTCCGCCATTTGTTGATCGTCCCCAAATATGAAGCAGCCGGAGTAAGCTCATCATCATCATCTTCTTCTTCTTCTTCTTTTACTCAGTCCATCGGAATTGCTTTAATTCATGGTTGTGTTGTGCAGGTTCCGCCGGTGATTGGGATCTTGACGAGGCAAGATTTAAGGCCATACAATATATTGAGTGCATTTCCTGATTTGGCAAGGATCAAAGGCAATGAAAAACGGAATTGAACTATGAAGATTTCTCAAACTCATTCATGACTATATTCAACACAAAGTATACACAACGATCTCCCACATATTCCATTGTTTTCTGCTGCAATTTGTTTCTTCTTCAAGTTCATATCATTGTTTCTTTCTATTATTTCTTTTCTTCTTCACCAATTTTGGGGCCTTCTATTAAATTCAATTTTACAAATAAACAAAATGGATTCTCCATTCAATTTTGATTCTGTTTGTTTTATATATATATATAGTTATGCTTAAATAAAATTTACCATCATTTTACCTCTACTTTCTTTACTTTC

Coding sequence (CDS)

ATGTTTGATGTCAGTGGTGTTTCTGTAAGTTACCATATCATGGACATTATTCCAGTTGCTATCATTGGCCTTCTTGGTGGATTCCTTGGAAGCCTTTACAATCATCTTCTCCACAAAATCCTCAGAGTTTACAACCTCATCAATCAGAAAGGGAGAATGCACAAACTATTACTAGCCTTAGCAGTATCACTTTTCACTTCAATTTGCCAATACAGTTTGCCATACCTTGTTCAATGCACTCCCTGCAACTCTTCTCTTTCAGATTCAGCTTGCCCAACCAATGGCCGGTCCGGCAACTTCAAGCAATTCAACTGTCCTAAAGGCTATTACAATGATCTCGCTACCCTTCTTCTCACCACCAACGACGACGCTGTTCGAAACATCTTCTCCATCAACACTCCAGCCGAGTATCAGCCTCTTTCCCTTGTCATCTTCTTCCTTCTCTACTGCATATTAGGGTTATTCACCTTCGGTATCGCCGTCCCTTCCGGTCTCTTCCTTCCGATCATCTTGATGGGCTCTGGCTATGGCCGCCTCATCGGCCTCCTCATGCGACCCTACACCAATCTCGATCAAGGACTCTTAGCTGTTCTCGGTGCTGCTTCCTTAATGGCCGGCTCCATGCGAATGACCGTTTCTCTTTGTGTCATATTCCTCGAACTCACCAACAATCTCCTTCTCCTTCCGATCACCATGATTGTTCTTCTAATTGCAAAAACAGTCGGAGACAGTTTTAATCCTAGCATTTACGACATCATTCTCCACCTCAAAGGACTTCCATTCCTCGACGCGAATCCGGAGCCATGGATGAGAAACATAACCGTCGGTGAGCTCGCCGACGCGAAACCAGCGGTGGTGACACTTCGGGGATTAGAGAAAGTATCACGCATTGTGGAGGTTCTTAGGAACACAACACACAATGGATTCCCTGTTGTGGATGCCGATGCGGTTGTGCCGCCGGTGGGCATGGCAGTGGGAGCGACGGAGCTGCACGGACTAGTACTGAGAGCGCATCTACTACAAGTGCTGAAGAAGAAATGGTTCTTGAGAGAGAGGAGGAGGACGGAGGACTGGGAAGTGAGGGAGAAATTTACATGGGTGGAATTGGCGGAGAGGGAAGGAAAGATCGAAGAGCTTGTAGTGACGAAAGAGGAAATGGAAATGTACGTTGATCTTCATCCATTGACGAACACTACACCGTATACAGTACTGGAAAGCATGTCGGTGGCAAAAGCTTTGGTTCTTTTCCGGCAAGTAGGACTCCGCCATTTGTTGATCGTCCCCAAATATGAAGCAGCCGGAGTTCCGCCGGTGATTGGGATCTTGACGAGGCAAGATTTAAGGCCATACAATATATTGAGTGCATTTCCTGATTTGGCAAGGATCAAAGGCAATGAAAAACGGAATTGA

Protein sequence

MFDVSGVSVSYHIMDIIPVAIIGLLGGFLGSLYNHLLHKILRVYNLINQKGRMHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYNDLATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILMGSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMIVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLEKVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRERRRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKALVLFRQVGLRHLLIVPKYEAAGVPPVIGILTRQDLRPYNILSAFPDLARIKGNEKRN

Homology

[Close]

BLAST of Cucsat.G4825 vs. ExPASy Swiss-Prot
Match: P92942 (Chloride channel protein CLC-b OS=Arabidopsis thaliana OX=3702 GN=CLC-B PE=1 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 5.4e-176
Identity = 322/442 (72.85%), Postives = 367/442 (83.03%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           +HK+LL+L VSLFTS+C Y LP+L +C PC+ S+ D  CPTNGRSGNFKQF+CPKGYYND
Sbjct: 367 IHKVLLSLTVSLFTSVCLYGLPFLAKCKPCDPSI-DEICPTNGRSGNFKQFHCPKGYYND 426

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRN+FS NTP E+   SL IFF+LYCILGLFTFGIA PSGLFLPIILM
Sbjct: 427 LATLLLTTNDDAVRNLFSSNTPNEFGMGSLWIFFVLYCILGLFTFGIATPSGLFLPIILM 486

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           G+ YGR++G  M  YT++DQGL AVLGAA+LMAGSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 487 GAAYGRMLGAAMGSYTSIDQGLYAVLGAAALMAGSMRMTVSLCVIFLELTNNLLLLPITM 546

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLIAKTVGDSFNPSIYDIILHLKGLPFL+ANPEPWMRN+TVGEL DAKP VVTL+G+E
Sbjct: 547 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLEANPEPWMRNLTVGELGDAKPPVVTLQGVE 606

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVS IV+VL+NTTHN FPV+D +A VP VG+A GATELHGL+LRAHL++VLKK+WFL E+
Sbjct: 607 KVSNIVDVLKNTTHNAFPVLD-EAEVPQVGLATGATELHGLILRAHLVKVLKKRWFLTEK 666

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTE+WEVREKF W ELAERE   +++ +T  EMEMYVDLHPLTNTTPYTV+E+MSVAKA
Sbjct: 667 RRTEEWEVREKFPWDELAEREDNFDDVAITSAEMEMYVDLHPLTNTTPYTVMENMSVAKA 726

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           LVLFRQVGLRHLLIVPK +A+G+                             PV+GILTR
Sbjct: 727 LVLFRQVGLRHLLIVPKIQASGMC----------------------------PVVGILTR 778

Query: 421 QDLRPYNILSAFPDLARIKGNE 443
           QDLR YNIL AFP L + KG +
Sbjct: 787 QDLRAYNILQAFPLLEKSKGGK 778

[Close]

BLAST of Cucsat.G4825 vs. ExPASy Swiss-Prot
Match: P92941 (Chloride channel protein CLC-a OS=Arabidopsis thaliana OX=3702 GN=CLC-A PE=1 SV=2)

HSP 1 Score: 587.4 bits (1513), Expect = 1.3e-166
Identity = 313/445 (70.34%), Postives = 355/445 (79.78%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           +HK+LL+L VSLFTS+C + LP+L +C PC+ S+ D  CPTNGRSGNFKQFNCP GYYND
Sbjct: 368 IHKVLLSLGVSLFTSVCLFGLPFLAECKPCDPSI-DEICPTNGRSGNFKQFNCPNGYYND 427

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           L+TLLLTTNDDAVRNIFS NTP E+  +SL IFF LYCILGL TFGIA PSGLFLPIILM
Sbjct: 428 LSTLLLTTNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLFLPIILM 487

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GS YGR++G  M  YTN+DQGL AVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 488 GSAYGRMLGTAMGSYTNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 547

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
            VLLIAKTVGDSFN SIY+IILHLKGLPFL+ANPEPWMRN+TVGEL DAKP VVTL G+E
Sbjct: 548 FVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVE 607

Query: 241 KVSRIVEVLRNTTHNGFPVVD-ADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRE 300
           KV+ IV+VLRNTTHN FPV+D AD            TELHGL+LRAHL++VLKK+WFL E
Sbjct: 608 KVANIVDVLRNTTHNAFPVLDGADQNT--------GTELHGLILRAHLVKVLKKRWFLNE 667

Query: 301 RRRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAK 360
           +RRTE+WEVREKFT VELAERE   +++ +T  EM++YVDLHPLTNTTPYTV++SMSVAK
Sbjct: 668 KRRTEEWEVREKFTPVELAEREDNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSMSVAK 727

Query: 361 ALVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILT 420
           ALVLFR VGLRHLL+VPK +A+G+S                            PVIGILT
Sbjct: 728 ALVLFRSVGLRHLLVVPKIQASGMS----------------------------PVIGILT 775

Query: 421 RQDLRPYNILSAFPDLARIKGNEKR 445
           RQDLR YNIL AFP L + K  + R
Sbjct: 788 RQDLRAYNILQAFPHLDKHKSGKAR 775

[Close]

BLAST of Cucsat.G4825 vs. ExPASy Swiss-Prot
Match: P60300 (Putative chloride channel-like protein CLC-g OS=Arabidopsis thaliana OX=3702 GN=CLC-G PE=1 SV=2)

HSP 1 Score: 376.7 bits (966), Expect = 3.6e-103
Identity = 210/446 (47.09%), Postives = 289/446 (64.80%), Query Frame = 0


Query: 3   KLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYNDLA 62
           K+LLA A+S+FTS   + LP+L  C PC     +  CPT GRSGNFK++ CP G+YNDLA
Sbjct: 354 KILLACAISIFTSCLLFGLPFLASCQPCPVDALEE-CPTIGRSGNFKKYQCPPGHYNDLA 413

Query: 63  TLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILMGS 122
           +L+  TNDDA++N+FS NT  E+   S+++FF+    L +F++GI  P+GLF+P+I+ G+
Sbjct: 414 SLIFNTNDDAIKNLFSKNTDFEFHYFSVLVFFVTCFFLSIFSYGIVAPAGLFVPVIVTGA 473

Query: 123 GYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMIV 182
            YGR +G+L+   +NL+ GL AVLGAAS + G+MRMTVS CVI LELTNNLLLLP+ M+V
Sbjct: 474 SYGRFVGMLLGSNSNLNHGLFAVLGAASFLGGTMRMTVSTCVILLELTNNLLLLPMMMVV 533

Query: 183 LLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLEKV 242
           LLI+KTV D FN +IY++I+ LKG P+L ++ EP+MR + VG++      V    G+EKV
Sbjct: 534 LLISKTVADGFNANIYNLIMKLKGFPYLYSHAEPYMRQLLVGDVVTGPLQV--FNGIEKV 593

Query: 243 SRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRERRR 302
             IV VL+ T HNGFPVVD     PP+     A  LHGL+LRAH+L +LKK+ F+     
Sbjct: 594 ETIVHVLKTTNHNGFPVVDG----PPL---AAAPVLHGLILRAHILTLLKKRVFMPSPVA 653

Query: 303 TEDWEVREKFTWVELAE----REGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVA 362
             D     +F   E A+    R  KIE++ +++EE+ MY+DLHP +N +PYTV+E+MS+A
Sbjct: 654 C-DSNTLSQFKAEEFAKKGSGRSDKIEDVELSEEELNMYLDLHPFSNASPYTVVETMSLA 713

Query: 363 KALVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGIL 422
           KAL+LFR+VG+RHLL++PK                                  PPV+GIL
Sbjct: 714 KALILFREVGIRHLLVIPK------------------------------TSNRPPVVGIL 758

Query: 423 TRQDLRPYNILSAFPDLARIKGNEKR 445
           TR D  P +IL   P ++R K    R
Sbjct: 774 TRHDFMPEHILGLHPSVSRSKWKRLR 758

[Close]

BLAST of Cucsat.G4825 vs. ExPASy Swiss-Prot
Match: Q96282 (Chloride channel protein CLC-c OS=Arabidopsis thaliana OX=3702 GN=CLC-C PE=1 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 8.3e-100
Identity = 203/441 (46.03%), Postives = 287/441 (65.08%), Query Frame = 0


Query: 3   KLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYNDLA 62
           K++L +AVS+ +S C + LP+L QCTPC   + +  CP+ GRS  +K F CP  +YNDL+
Sbjct: 379 KIMLVMAVSILSSCCAFGLPWLSQCTPCPIGIEEGKCPSVGRSSIYKSFQCPPNHYNDLS 438

Query: 63  TLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILMGS 122
           +LLL TNDDA+RN+F+  +  E+   +L IFF+    LG+ T+GIA+PSGLF+P+IL G+
Sbjct: 439 SLLLNTNDDAIRNLFTSRSENEFHISTLAIFFVAVYCLGIITYGIAIPSGLFIPVILAGA 498

Query: 123 GYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMIV 182
            YGRL+G L+ P + LD GL ++LGAAS + G+MRMTVSLCVI LELTNNLL+LP+ M+V
Sbjct: 499 SYGRLVGRLLGPVSQLDVGLFSLLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLVMLV 558

Query: 183 LLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLEKV 242
           LLI+KTV D FN  +YD I+ +KGLP+++ + EP+MRN+   ++     A+++   +EKV
Sbjct: 559 LLISKTVADCFNRGVYDQIVTMKGLPYMEDHAEPYMRNLVAKDVVSG--ALISFSRVEKV 618

Query: 243 SRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRERRR 302
             I + L+ T HNGFPV+D     PP      A+EL G+ LR+HLL +L+ K F ++R  
Sbjct: 619 GVIWQALKMTRHNGFPVIDE----PPF---TEASELCGIALRSHLLVLLQGKKFSKQRTT 678

Query: 303 TEDWEVR----EKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVA 362
                +R      F    L  +  KIE+L +++EEMEMYVDLHP+TNT+PYTVLE++S+A
Sbjct: 679 FGSQILRSCKARDFGKAGLG-KGLKIEDLDLSEEEMEMYVDLHPITNTSPYTVLETLSLA 738

Query: 363 KALVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGIL 422
           KA +LFRQ+GLRHL +VPK                                  PP++GIL
Sbjct: 739 KAAILFRQLGLRHLCVVPKTPGR------------------------------PPIVGIL 779

Query: 423 TRQDLRPYNILSAFPDLARIK 440
           TR D  P ++L  +P +  +K
Sbjct: 799 TRHDFMPEHVLGLYPHIDPLK 779

[Close]

BLAST of Cucsat.G4825 vs. ExPASy Swiss-Prot
Match: P92943 (Chloride channel protein CLC-d OS=Arabidopsis thaliana OX=3702 GN=CLC-D PE=1 SV=2)

HSP 1 Score: 264.6 bits (675), Expect = 2.0e-69
Identity = 164/387 (42.38%), Postives = 239/387 (61.76%), Query Frame = 0


Query: 3   KLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSA--CP-TNGRSGNFKQFNC-PKGYY 62
           K++ A  +S  TS   + LP L +C+PC  S+ DS   CP   G  GN+  F C     Y
Sbjct: 360 KIIEACIISCITSAISFGLPLLRKCSPCPESVPDSGIECPRPPGMYGNYVNFFCKTDNEY 419

Query: 63  NDLATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPII 122
           NDLAT+   T DDA+RN+FS  T  E+   SL+ F  ++  L + TFG AVP+G F+P I
Sbjct: 420 NDLATIFFNTQDDAIRNLFSAKTMREFSAQSLLTFLAMFYTLAVVTFGTAVPAGQFVPGI 479

Query: 123 LMGSGYGRLIGL-LMRPY--TNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLL 182
           ++GS YGRL+G+ ++R Y   N+++G  A+LGAAS + GSMRMTVSLCVI +E+TNNL L
Sbjct: 480 MIGSTYGRLVGMFVVRFYKKLNIEEGTYALLGAASFLGGSMRMTVSLCVIMVEITNNLKL 539

Query: 183 LPITMIVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVT 242
           LP+ M+VLLI+K VGD+FN  +Y++   LKG+P L++ P+  MR +   E   ++  V++
Sbjct: 540 LPLIMLVLLISKAVGDAFNEGLYEVQARLKGIPLLESRPKYHMRQMIAKEACQSQ-KVIS 599

Query: 243 LRGLEKVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKW 302
           L  + +V+ +  +L +  HNGFPV+D            G T + GLVLR+HLL +L+ K 
Sbjct: 600 LPRVIRVADVASILGSNKHNGFPVID--------HTRSGETLVIGLVLRSHLLVLLQSKV 659

Query: 303 FLRERRRTEDWEVRE-KFTWVELAEREGK----IEELVVTKEEMEMYVDLHPLTNTTPYT 362
             +      D   R  + ++ E A+        IE++ +T +++EMY+DL P  N +PY 
Sbjct: 660 DFQHSPLPCDPSARNIRHSFSEFAKPVSSKGLCIEDIHLTSDDLEMYIDLAPFLNPSPYV 719

Query: 363 VLESMSVAKALVLFRQVGLRHLLIVPK 378
           V E MS+ K   LFRQ+GLRHL +VP+
Sbjct: 720 VPEDMSLTKVYNLFRQLGLRHLFVVPR 737

[Close]

BLAST of Cucsat.G4825 vs. NCBI nr
Match: NP_001267676.1 (chloride channel protein CLC-b-like [Cucumis sativus] >AFJ15538.1 chloride channel a [Cucumis sativus] >KGN63173.1 hypothetical protein Csa_021818 [Cucumis sativus])

HSP 1 Score: 786 bits (2030), Expect = 1.06e-279
Identity = 417/445 (93.71%), Postives = 417/445 (93.71%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND
Sbjct: 373 MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 432

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM
Sbjct: 433 LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 492

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 493 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 552

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE
Sbjct: 553 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 612

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER
Sbjct: 613 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 672

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA
Sbjct: 673 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 732

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           LVLFRQVGLRHLLIVPKYEAAGV                            PPVIGILTR
Sbjct: 733 LVLFRQVGLRHLLIVPKYEAAGV----------------------------PPVIGILTR 789

Query: 421 QDLRPYNILSAFPDLARIKGNEKRN 445
           QDLRPYNILSAFPDLARIKGNEKRN
Sbjct: 793 QDLRPYNILSAFPDLARIKGNEKRN 789

[Close]

BLAST of Cucsat.G4825 vs. NCBI nr
Match: XP_038885188.1 (chloride channel protein CLC-b isoform X2 [Benincasa hispida])

HSP 1 Score: 766 bits (1978), Expect = 8.31e-272
Identity = 405/445 (91.01%), Postives = 411/445 (92.36%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           MHKLLL+L VSLFTSICQYSLPYLVQCTPCN SL DSACPTNGRSGNFKQFNCPKG+YND
Sbjct: 373 MHKLLLSLGVSLFTSICQYSLPYLVQCTPCNPSLPDSACPTNGRSGNFKQFNCPKGHYND 432

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYC+LGL TFGIAVPSGLFLPIILM
Sbjct: 433 LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCVLGLITFGIAVPSGLFLPIILM 492

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 493 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 552

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPE WMRNITVGELADAKPAVVTLRGLE
Sbjct: 553 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPETWMRNITVGELADAKPAVVTLRGLE 612

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER
Sbjct: 613 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 672

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTE+WEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTV+ESMSVAKA
Sbjct: 673 RRTEEWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVMESMSVAKA 732

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           +VLFRQVGLRHLLIVPKYEAAGV                            PPVIGILTR
Sbjct: 733 MVLFRQVGLRHLLIVPKYEAAGV----------------------------PPVIGILTR 789

Query: 421 QDLRPYNILSAFPDLARIKGNEKRN 445
           QDLRPYNILSAFP LARIKGNEKRN
Sbjct: 793 QDLRPYNILSAFPHLARIKGNEKRN 789

[Close]

BLAST of Cucsat.G4825 vs. NCBI nr
Match: XP_008441347.1 (PREDICTED: chloride channel protein CLC-b [Cucumis melo] >KAA0056578.1 chloride channel protein CLC-b [Cucumis melo var. makuwa] >TYK24260.1 chloride channel protein CLC-b [Cucumis melo var. makuwa])

HSP 1 Score: 765 bits (1976), Expect = 1.67e-271
Identity = 406/445 (91.24%), Postives = 410/445 (92.13%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           MHKLLLAL VSLFTSICQYSLPYLVQCTPCN SL DSACPTNGRSGNFKQFNCPKG+YND
Sbjct: 373 MHKLLLALGVSLFTSICQYSLPYLVQCTPCNLSLPDSACPTNGRSGNFKQFNCPKGHYND 432

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGL TFGIAVPSGLFLPIILM
Sbjct: 433 LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLITFGIAVPSGLFLPIILM 492

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GSGYGRLIGLLMR YTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 493 GSGYGRLIGLLMRSYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 552

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVV LRGLE
Sbjct: 553 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVILRGLE 612

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVSRIVEVLRNTTHNGFPVVDAD+VVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER
Sbjct: 613 KVSRIVEVLRNTTHNGFPVVDADSVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 672

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTE+WEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTV+ESMSVAKA
Sbjct: 673 RRTEEWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVMESMSVAKA 732

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           LVLFRQVGLRHLLIVPKYEAAGV                            PPVIGILTR
Sbjct: 733 LVLFRQVGLRHLLIVPKYEAAGV----------------------------PPVIGILTR 789

Query: 421 QDLRPYNILSAFPDLARIKGNEKRN 445
           QDLRPYNILSAFPDLAR KGNEKRN
Sbjct: 793 QDLRPYNILSAFPDLARTKGNEKRN 789

[Close]

BLAST of Cucsat.G4825 vs. NCBI nr
Match: XP_022991119.1 (chloride channel protein CLC-b-like [Cucurbita maxima])

HSP 1 Score: 736 bits (1901), Expect = 4.02e-260
Identity = 387/445 (86.97%), Postives = 403/445 (90.56%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           M KLLL+L VSLFTSICQYSLPYLVQCTPCN SL DS CPTNGRSGNFKQFNCPKG+YND
Sbjct: 373 MQKLLLSLGVSLFTSICQYSLPYLVQCTPCNPSLPDSVCPTNGRSGNFKQFNCPKGHYND 432

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRNIFSINTPAE+QPLSLVIFFLLYC+LGL TFGIAVPSGLFLPIILM
Sbjct: 433 LATLLLTTNDDAVRNIFSINTPAEFQPLSLVIFFLLYCVLGLITFGIAVPSGLFLPIILM 492

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GSGYGRLIGLLM+PYTNLDQGLLAVLGAASLM+GSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 493 GSGYGRLIGLLMKPYTNLDQGLLAVLGAASLMSGSMRMTVSLCVIFLELTNNLLLLPITM 552

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLI+KTVGDSFNPSIY+IILHLKGLPFLDANPE WMRNITVG+LADAKPAVVTLRGLE
Sbjct: 553 IVLLISKTVGDSFNPSIYEIILHLKGLPFLDANPETWMRNITVGDLADAKPAVVTLRGLE 612

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVSRIVEVL+NTTHNGFPVVDA+AVVPPVGMAVGATELHG+VLR HLLQ+LKKKWFLRER
Sbjct: 613 KVSRIVEVLKNTTHNGFPVVDAEAVVPPVGMAVGATELHGVVLRKHLLQLLKKKWFLRER 672

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTE+WEVREKFTWVELAEREGKIEEL VTKEEMEMYVDLHPLTNTTPYTV+ESMSVAKA
Sbjct: 673 RRTEEWEVREKFTWVELAEREGKIEELAVTKEEMEMYVDLHPLTNTTPYTVIESMSVAKA 732

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           LVLFRQVGLRHLLIVPKYEAAGV                            PPVIGILTR
Sbjct: 733 LVLFRQVGLRHLLIVPKYEAAGV----------------------------PPVIGILTR 789

Query: 421 QDLRPYNILSAFPDLARIKGNEKRN 445
           QDLRPYNIL+AFP L RIK N K N
Sbjct: 793 QDLRPYNILTAFPHLTRIKSNHKGN 789

[Close]

BLAST of Cucsat.G4825 vs. NCBI nr
Match: XP_022955252.1 (chloride channel protein CLC-b-like [Cucurbita moschata])

HSP 1 Score: 736 bits (1901), Expect = 4.02e-260
Identity = 387/445 (86.97%), Postives = 403/445 (90.56%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           M KLLL+L VSLFTSICQYSLPYLVQCTPCN SL DS CPTNGRSGNFKQFNCPKG+YND
Sbjct: 373 MQKLLLSLGVSLFTSICQYSLPYLVQCTPCNPSLPDSVCPTNGRSGNFKQFNCPKGHYND 432

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRNIFSINTPAE+QPLSLVIFFLLYC+LGL TFGIAVPSGLFLPIILM
Sbjct: 433 LATLLLTTNDDAVRNIFSINTPAEFQPLSLVIFFLLYCVLGLITFGIAVPSGLFLPIILM 492

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GSGYGRLIGLLM+PYTNLDQGLLAVLGAASLM+GSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 493 GSGYGRLIGLLMKPYTNLDQGLLAVLGAASLMSGSMRMTVSLCVIFLELTNNLLLLPITM 552

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLI+KTVGDSFNPSIY+IILHLKGLPFLDANPE WMRNITVG+LADAKPAVVTLRGLE
Sbjct: 553 IVLLISKTVGDSFNPSIYEIILHLKGLPFLDANPETWMRNITVGDLADAKPAVVTLRGLE 612

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVSRIVEVL+NTTHNGFPVVDA+AVVPPVGMAVGATELHG+VLR HLLQ+LKKKWFLRER
Sbjct: 613 KVSRIVEVLKNTTHNGFPVVDAEAVVPPVGMAVGATELHGVVLRKHLLQLLKKKWFLRER 672

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTE+WEVREKFTWVELAEREGKIEEL VTKEEMEMYVDLHPLTNTTPYTV+ESMSVAKA
Sbjct: 673 RRTEEWEVREKFTWVELAEREGKIEELAVTKEEMEMYVDLHPLTNTTPYTVIESMSVAKA 732

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           LVLFRQVGLRHLLIVPKYEAAGV                            PPVIGILTR
Sbjct: 733 LVLFRQVGLRHLLIVPKYEAAGV----------------------------PPVIGILTR 789

Query: 421 QDLRPYNILSAFPDLARIKGNEKRN 445
           QDLRPYNIL+AFP L RIK N K N
Sbjct: 793 QDLRPYNILTAFPHLTRIKSNHKGN 789

[Close]

BLAST of Cucsat.G4825 vs. ExPASy TrEMBL
Match: I1Z8C8 (Chloride channel protein OS=Cucumis sativus OX=3659 GN=ClCa PE=2 SV=1)

HSP 1 Score: 786 bits (2030), Expect = 5.13e-280
Identity = 417/445 (93.71%), Postives = 417/445 (93.71%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND
Sbjct: 373 MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 432

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM
Sbjct: 433 LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 492

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 493 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 552

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE
Sbjct: 553 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 612

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER
Sbjct: 613 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 672

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA
Sbjct: 673 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 732

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           LVLFRQVGLRHLLIVPKYEAAGV                            PPVIGILTR
Sbjct: 733 LVLFRQVGLRHLLIVPKYEAAGV----------------------------PPVIGILTR 789

Query: 421 QDLRPYNILSAFPDLARIKGNEKRN 445
           QDLRPYNILSAFPDLARIKGNEKRN
Sbjct: 793 QDLRPYNILSAFPDLARIKGNEKRN 789

[Close]

BLAST of Cucsat.G4825 vs. ExPASy TrEMBL
Match: A0A5D3DLW5 (Chloride channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00230 PE=3 SV=1)

HSP 1 Score: 765 bits (1976), Expect = 8.09e-272
Identity = 406/445 (91.24%), Postives = 410/445 (92.13%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           MHKLLLAL VSLFTSICQYSLPYLVQCTPCN SL DSACPTNGRSGNFKQFNCPKG+YND
Sbjct: 373 MHKLLLALGVSLFTSICQYSLPYLVQCTPCNLSLPDSACPTNGRSGNFKQFNCPKGHYND 432

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGL TFGIAVPSGLFLPIILM
Sbjct: 433 LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLITFGIAVPSGLFLPIILM 492

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GSGYGRLIGLLMR YTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 493 GSGYGRLIGLLMRSYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 552

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVV LRGLE
Sbjct: 553 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVILRGLE 612

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVSRIVEVLRNTTHNGFPVVDAD+VVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER
Sbjct: 613 KVSRIVEVLRNTTHNGFPVVDADSVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 672

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTE+WEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTV+ESMSVAKA
Sbjct: 673 RRTEEWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVMESMSVAKA 732

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           LVLFRQVGLRHLLIVPKYEAAGV                            PPVIGILTR
Sbjct: 733 LVLFRQVGLRHLLIVPKYEAAGV----------------------------PPVIGILTR 789

Query: 421 QDLRPYNILSAFPDLARIKGNEKRN 445
           QDLRPYNILSAFPDLAR KGNEKRN
Sbjct: 793 QDLRPYNILSAFPDLARTKGNEKRN 789

[Close]

BLAST of Cucsat.G4825 vs. ExPASy TrEMBL
Match: A0A1S3B373 (Chloride channel protein OS=Cucumis melo OX=3656 GN=LOC103485488 PE=3 SV=1)

HSP 1 Score: 765 bits (1976), Expect = 8.09e-272
Identity = 406/445 (91.24%), Postives = 410/445 (92.13%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           MHKLLLAL VSLFTSICQYSLPYLVQCTPCN SL DSACPTNGRSGNFKQFNCPKG+YND
Sbjct: 373 MHKLLLALGVSLFTSICQYSLPYLVQCTPCNLSLPDSACPTNGRSGNFKQFNCPKGHYND 432

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGL TFGIAVPSGLFLPIILM
Sbjct: 433 LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLITFGIAVPSGLFLPIILM 492

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GSGYGRLIGLLMR YTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 493 GSGYGRLIGLLMRSYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 552

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVV LRGLE
Sbjct: 553 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVILRGLE 612

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVSRIVEVLRNTTHNGFPVVDAD+VVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER
Sbjct: 613 KVSRIVEVLRNTTHNGFPVVDADSVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 672

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTE+WEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTV+ESMSVAKA
Sbjct: 673 RRTEEWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVMESMSVAKA 732

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           LVLFRQVGLRHLLIVPKYEAAGV                            PPVIGILTR
Sbjct: 733 LVLFRQVGLRHLLIVPKYEAAGV----------------------------PPVIGILTR 789

Query: 421 QDLRPYNILSAFPDLARIKGNEKRN 445
           QDLRPYNILSAFPDLAR KGNEKRN
Sbjct: 793 QDLRPYNILSAFPDLARTKGNEKRN 789

[Close]

BLAST of Cucsat.G4825 vs. ExPASy TrEMBL
Match: A0A6J1JTX8 (chloride channel protein CLC-b-like OS=Cucurbita maxima OX=3661 GN=LOC111487811 PE=3 SV=1)

HSP 1 Score: 736 bits (1901), Expect = 1.95e-260
Identity = 387/445 (86.97%), Postives = 403/445 (90.56%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           M KLLL+L VSLFTSICQYSLPYLVQCTPCN SL DS CPTNGRSGNFKQFNCPKG+YND
Sbjct: 373 MQKLLLSLGVSLFTSICQYSLPYLVQCTPCNPSLPDSVCPTNGRSGNFKQFNCPKGHYND 432

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRNIFSINTPAE+QPLSLVIFFLLYC+LGL TFGIAVPSGLFLPIILM
Sbjct: 433 LATLLLTTNDDAVRNIFSINTPAEFQPLSLVIFFLLYCVLGLITFGIAVPSGLFLPIILM 492

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GSGYGRLIGLLM+PYTNLDQGLLAVLGAASLM+GSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 493 GSGYGRLIGLLMKPYTNLDQGLLAVLGAASLMSGSMRMTVSLCVIFLELTNNLLLLPITM 552

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLI+KTVGDSFNPSIY+IILHLKGLPFLDANPE WMRNITVG+LADAKPAVVTLRGLE
Sbjct: 553 IVLLISKTVGDSFNPSIYEIILHLKGLPFLDANPETWMRNITVGDLADAKPAVVTLRGLE 612

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVSRIVEVL+NTTHNGFPVVDA+AVVPPVGMAVGATELHG+VLR HLLQ+LKKKWFLRER
Sbjct: 613 KVSRIVEVLKNTTHNGFPVVDAEAVVPPVGMAVGATELHGVVLRKHLLQLLKKKWFLRER 672

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTE+WEVREKFTWVELAEREGKIEEL VTKEEMEMYVDLHPLTNTTPYTV+ESMSVAKA
Sbjct: 673 RRTEEWEVREKFTWVELAEREGKIEELAVTKEEMEMYVDLHPLTNTTPYTVIESMSVAKA 732

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           LVLFRQVGLRHLLIVPKYEAAGV                            PPVIGILTR
Sbjct: 733 LVLFRQVGLRHLLIVPKYEAAGV----------------------------PPVIGILTR 789

Query: 421 QDLRPYNILSAFPDLARIKGNEKRN 445
           QDLRPYNIL+AFP L RIK N K N
Sbjct: 793 QDLRPYNILTAFPHLTRIKSNHKGN 789

[Close]

BLAST of Cucsat.G4825 vs. ExPASy TrEMBL
Match: A0A6J1GT18 (chloride channel protein CLC-b-like OS=Cucurbita moschata OX=3662 GN=LOC111457265 PE=3 SV=1)

HSP 1 Score: 736 bits (1901), Expect = 1.95e-260
Identity = 387/445 (86.97%), Postives = 403/445 (90.56%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           M KLLL+L VSLFTSICQYSLPYLVQCTPCN SL DS CPTNGRSGNFKQFNCPKG+YND
Sbjct: 373 MQKLLLSLGVSLFTSICQYSLPYLVQCTPCNPSLPDSVCPTNGRSGNFKQFNCPKGHYND 432

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRNIFSINTPAE+QPLSLVIFFLLYC+LGL TFGIAVPSGLFLPIILM
Sbjct: 433 LATLLLTTNDDAVRNIFSINTPAEFQPLSLVIFFLLYCVLGLITFGIAVPSGLFLPIILM 492

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GSGYGRLIGLLM+PYTNLDQGLLAVLGAASLM+GSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 493 GSGYGRLIGLLMKPYTNLDQGLLAVLGAASLMSGSMRMTVSLCVIFLELTNNLLLLPITM 552

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLI+KTVGDSFNPSIY+IILHLKGLPFLDANPE WMRNITVG+LADAKPAVVTLRGLE
Sbjct: 553 IVLLISKTVGDSFNPSIYEIILHLKGLPFLDANPETWMRNITVGDLADAKPAVVTLRGLE 612

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVSRIVEVL+NTTHNGFPVVDA+AVVPPVGMAVGATELHG+VLR HLLQ+LKKKWFLRER
Sbjct: 613 KVSRIVEVLKNTTHNGFPVVDAEAVVPPVGMAVGATELHGVVLRKHLLQLLKKKWFLRER 672

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTE+WEVREKFTWVELAEREGKIEEL VTKEEMEMYVDLHPLTNTTPYTV+ESMSVAKA
Sbjct: 673 RRTEEWEVREKFTWVELAEREGKIEELAVTKEEMEMYVDLHPLTNTTPYTVIESMSVAKA 732

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           LVLFRQVGLRHLLIVPKYEAAGV                            PPVIGILTR
Sbjct: 733 LVLFRQVGLRHLLIVPKYEAAGV----------------------------PPVIGILTR 789

Query: 421 QDLRPYNILSAFPDLARIKGNEKRN 445
           QDLRPYNIL+AFP L RIK N K N
Sbjct: 793 QDLRPYNILTAFPHLTRIKSNHKGN 789

[Close]

BLAST of Cucsat.G4825 vs. TAIR 10
Match: AT3G27170.1 (chloride channel B )

HSP 1 Score: 618.6 bits (1594), Expect = 3.9e-177
Identity = 322/442 (72.85%), Postives = 367/442 (83.03%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           +HK+LL+L VSLFTS+C Y LP+L +C PC+ S+ D  CPTNGRSGNFKQF+CPKGYYND
Sbjct: 367 IHKVLLSLTVSLFTSVCLYGLPFLAKCKPCDPSI-DEICPTNGRSGNFKQFHCPKGYYND 426

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           LATLLLTTNDDAVRN+FS NTP E+   SL IFF+LYCILGLFTFGIA PSGLFLPIILM
Sbjct: 427 LATLLLTTNDDAVRNLFSSNTPNEFGMGSLWIFFVLYCILGLFTFGIATPSGLFLPIILM 486

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           G+ YGR++G  M  YT++DQGL AVLGAA+LMAGSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 487 GAAYGRMLGAAMGSYTSIDQGLYAVLGAAALMAGSMRMTVSLCVIFLELTNNLLLLPITM 546

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
           IVLLIAKTVGDSFNPSIYDIILHLKGLPFL+ANPEPWMRN+TVGEL DAKP VVTL+G+E
Sbjct: 547 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLEANPEPWMRNLTVGELGDAKPPVVTLQGVE 606

Query: 241 KVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRER 300
           KVS IV+VL+NTTHN FPV+D +A VP VG+A GATELHGL+LRAHL++VLKK+WFL E+
Sbjct: 607 KVSNIVDVLKNTTHNAFPVLD-EAEVPQVGLATGATELHGLILRAHLVKVLKKRWFLTEK 666

Query: 301 RRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKA 360
           RRTE+WEVREKF W ELAERE   +++ +T  EMEMYVDLHPLTNTTPYTV+E+MSVAKA
Sbjct: 667 RRTEEWEVREKFPWDELAEREDNFDDVAITSAEMEMYVDLHPLTNTTPYTVMENMSVAKA 726

Query: 361 LVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILTR 420
           LVLFRQVGLRHLLIVPK +A+G+                             PV+GILTR
Sbjct: 727 LVLFRQVGLRHLLIVPKIQASGMC----------------------------PVVGILTR 778

Query: 421 QDLRPYNILSAFPDLARIKGNE 443
           QDLR YNIL AFP L + KG +
Sbjct: 787 QDLRAYNILQAFPLLEKSKGGK 778

[Close]

BLAST of Cucsat.G4825 vs. TAIR 10
Match: AT5G40890.1 (chloride channel A )

HSP 1 Score: 587.4 bits (1513), Expect = 9.5e-168
Identity = 313/445 (70.34%), Postives = 355/445 (79.78%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           +HK+LL+L VSLFTS+C + LP+L +C PC+ S+ D  CPTNGRSGNFKQFNCP GYYND
Sbjct: 368 IHKVLLSLGVSLFTSVCLFGLPFLAECKPCDPSI-DEICPTNGRSGNFKQFNCPNGYYND 427

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           L+TLLLTTNDDAVRNIFS NTP E+  +SL IFF LYCILGL TFGIA PSGLFLPIILM
Sbjct: 428 LSTLLLTTNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLFLPIILM 487

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GS YGR++G  M  YTN+DQGL AVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 488 GSAYGRMLGTAMGSYTNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 547

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
            VLLIAKTVGDSFN SIY+IILHLKGLPFL+ANPEPWMRN+TVGEL DAKP VVTL G+E
Sbjct: 548 FVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVE 607

Query: 241 KVSRIVEVLRNTTHNGFPVVD-ADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRE 300
           KV+ IV+VLRNTTHN FPV+D AD            TELHGL+LRAHL++VLKK+WFL E
Sbjct: 608 KVANIVDVLRNTTHNAFPVLDGADQNT--------GTELHGLILRAHLVKVLKKRWFLNE 667

Query: 301 RRRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAK 360
           +RRTE+WEVREKFT VELAERE   +++ +T  EM++YVDLHPLTNTTPYTV++SMSVAK
Sbjct: 668 KRRTEEWEVREKFTPVELAEREDNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSMSVAK 727

Query: 361 ALVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILT 420
           ALVLFR VGLRHLL+VPK +A+G+S                            PVIGILT
Sbjct: 728 ALVLFRSVGLRHLLVVPKIQASGMS----------------------------PVIGILT 775

Query: 421 RQDLRPYNILSAFPDLARIKGNEKR 445
           RQDLR YNIL AFP L + K  + R
Sbjct: 788 RQDLRAYNILQAFPHLDKHKSGKAR 775

[Close]

BLAST of Cucsat.G4825 vs. TAIR 10
Match: AT5G40890.2 (chloride channel A )

HSP 1 Score: 587.4 bits (1513), Expect = 9.5e-168
Identity = 313/445 (70.34%), Postives = 355/445 (79.78%), Query Frame = 0


Query: 1   MHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYND 60
           +HK+LL+L VSLFTS+C + LP+L +C PC+ S+ D  CPTNGRSGNFKQFNCP GYYND
Sbjct: 236 IHKVLLSLGVSLFTSVCLFGLPFLAECKPCDPSI-DEICPTNGRSGNFKQFNCPNGYYND 295

Query: 61  LATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILM 120
           L+TLLLTTNDDAVRNIFS NTP E+  +SL IFF LYCILGL TFGIA PSGLFLPIILM
Sbjct: 296 LSTLLLTTNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLFLPIILM 355

Query: 121 GSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 180
           GS YGR++G  M  YTN+DQGL AVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM
Sbjct: 356 GSAYGRMLGTAMGSYTNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITM 415

Query: 181 IVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLE 240
            VLLIAKTVGDSFN SIY+IILHLKGLPFL+ANPEPWMRN+TVGEL DAKP VVTL G+E
Sbjct: 416 FVLLIAKTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVE 475

Query: 241 KVSRIVEVLRNTTHNGFPVVD-ADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRE 300
           KV+ IV+VLRNTTHN FPV+D AD            TELHGL+LRAHL++VLKK+WFL E
Sbjct: 476 KVANIVDVLRNTTHNAFPVLDGADQNT--------GTELHGLILRAHLVKVLKKRWFLNE 535

Query: 301 RRRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAK 360
           +RRTE+WEVREKFT VELAERE   +++ +T  EM++YVDLHPLTNTTPYTV++SMSVAK
Sbjct: 536 KRRTEEWEVREKFTPVELAEREDNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSMSVAK 595

Query: 361 ALVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGILT 420
           ALVLFR VGLRHLL+VPK +A+G+S                            PVIGILT
Sbjct: 596 ALVLFRSVGLRHLLVVPKIQASGMS----------------------------PVIGILT 643

Query: 421 RQDLRPYNILSAFPDLARIKGNEKR 445
           RQDLR YNIL AFP L + K  + R
Sbjct: 656 RQDLRAYNILQAFPHLDKHKSGKAR 643

[Close]

BLAST of Cucsat.G4825 vs. TAIR 10
Match: AT5G33280.1 (Voltage-gated chloride channel family protein )

HSP 1 Score: 376.7 bits (966), Expect = 2.6e-104
Identity = 210/446 (47.09%), Postives = 289/446 (64.80%), Query Frame = 0


Query: 3   KLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYNDLA 62
           K+LLA A+S+FTS   + LP+L  C PC     +  CPT GRSGNFK++ CP G+YNDLA
Sbjct: 354 KILLACAISIFTSCLLFGLPFLASCQPCPVDALEE-CPTIGRSGNFKKYQCPPGHYNDLA 413

Query: 63  TLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILMGS 122
           +L+  TNDDA++N+FS NT  E+   S+++FF+    L +F++GI  P+GLF+P+I+ G+
Sbjct: 414 SLIFNTNDDAIKNLFSKNTDFEFHYFSVLVFFVTCFFLSIFSYGIVAPAGLFVPVIVTGA 473

Query: 123 GYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMIV 182
            YGR +G+L+   +NL+ GL AVLGAAS + G+MRMTVS CVI LELTNNLLLLP+ M+V
Sbjct: 474 SYGRFVGMLLGSNSNLNHGLFAVLGAASFLGGTMRMTVSTCVILLELTNNLLLLPMMMVV 533

Query: 183 LLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLEKV 242
           LLI+KTV D FN +IY++I+ LKG P+L ++ EP+MR + VG++      V    G+EKV
Sbjct: 534 LLISKTVADGFNANIYNLIMKLKGFPYLYSHAEPYMRQLLVGDVVTGPLQV--FNGIEKV 593

Query: 243 SRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRERRR 302
             IV VL+ T HNGFPVVD     PP+     A  LHGL+LRAH+L +LKK+ F+     
Sbjct: 594 ETIVHVLKTTNHNGFPVVDG----PPL---AAAPVLHGLILRAHILTLLKKRVFMPSPVA 653

Query: 303 TEDWEVREKFTWVELAE----REGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVA 362
             D     +F   E A+    R  KIE++ +++EE+ MY+DLHP +N +PYTV+E+MS+A
Sbjct: 654 C-DSNTLSQFKAEEFAKKGSGRSDKIEDVELSEEELNMYLDLHPFSNASPYTVVETMSLA 713

Query: 363 KALVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGIL 422
           KAL+LFR+VG+RHLL++PK                                  PPV+GIL
Sbjct: 714 KALILFREVGIRHLLVIPK------------------------------TSNRPPVVGIL 758

Query: 423 TRQDLRPYNILSAFPDLARIKGNEKR 445
           TR D  P +IL   P ++R K    R
Sbjct: 774 TRHDFMPEHILGLHPSVSRSKWKRLR 758

[Close]

BLAST of Cucsat.G4825 vs. TAIR 10
Match: AT5G49890.1 (chloride channel C )

HSP 1 Score: 365.5 bits (937), Expect = 5.9e-101
Identity = 203/441 (46.03%), Postives = 287/441 (65.08%), Query Frame = 0


Query: 3   KLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYNDLA 62
           K++L +AVS+ +S C + LP+L QCTPC   + +  CP+ GRS  +K F CP  +YNDL+
Sbjct: 379 KIMLVMAVSILSSCCAFGLPWLSQCTPCPIGIEEGKCPSVGRSSIYKSFQCPPNHYNDLS 438

Query: 63  TLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILMGS 122
           +LLL TNDDA+RN+F+  +  E+   +L IFF+    LG+ T+GIA+PSGLF+P+IL G+
Sbjct: 439 SLLLNTNDDAIRNLFTSRSENEFHISTLAIFFVAVYCLGIITYGIAIPSGLFIPVILAGA 498

Query: 123 GYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMIV 182
            YGRL+G L+ P + LD GL ++LGAAS + G+MRMTVSLCVI LELTNNLL+LP+ M+V
Sbjct: 499 SYGRLVGRLLGPVSQLDVGLFSLLGAASFLGGTMRMTVSLCVILLELTNNLLMLPLVMLV 558

Query: 183 LLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLEKV 242
           LLI+KTV D FN  +YD I+ +KGLP+++ + EP+MRN+   ++     A+++   +EKV
Sbjct: 559 LLISKTVADCFNRGVYDQIVTMKGLPYMEDHAEPYMRNLVAKDVVSG--ALISFSRVEKV 618

Query: 243 SRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRERRR 302
             I + L+ T HNGFPV+D     PP      A+EL G+ LR+HLL +L+ K F ++R  
Sbjct: 619 GVIWQALKMTRHNGFPVIDE----PPF---TEASELCGIALRSHLLVLLQGKKFSKQRTT 678

Query: 303 TEDWEVR----EKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVA 362
                +R      F    L  +  KIE+L +++EEMEMYVDLHP+TNT+PYTVLE++S+A
Sbjct: 679 FGSQILRSCKARDFGKAGLG-KGLKIEDLDLSEEEMEMYVDLHPITNTSPYTVLETLSLA 738

Query: 363 KALVLFRQVGLRHLLIVPKYEAAGVSSSSSSSSSSSFTQSIGIALIHGCVVQVPPVIGIL 422
           KA +LFRQ+GLRHL +VPK                                  PP++GIL
Sbjct: 739 KAAILFRQLGLRHLCVVPKTPGR------------------------------PPIVGIL 779

Query: 423 TRQDLRPYNILSAFPDLARIK 440
           TR D  P ++L  +P +  +K
Sbjct: 799 TRHDFMPEHVLGLYPHIDPLK 779

The following BLAST results are available for this feature:

BLAST of Cucsat.G4825 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P92942	5.4e-176	72.85	Chloride channel protein CLC-b OS=Arabidopsis thaliana OX=3702 GN=CLC-B PE=1 SV=...	[more]
P92941	1.3e-166	70.34	Chloride channel protein CLC-a OS=Arabidopsis thaliana OX=3702 GN=CLC-A PE=1 SV=...	[more]
P60300	3.6e-103	47.09	Putative chloride channel-like protein CLC-g OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q96282	8.3e-100	46.03	Chloride channel protein CLC-c OS=Arabidopsis thaliana OX=3702 GN=CLC-C PE=1 SV=...	[more]
P92943	2.0e-69	42.38	Chloride channel protein CLC-d OS=Arabidopsis thaliana OX=3702 GN=CLC-D PE=1 SV=...	[more]

BLAST of Cucsat.G4825 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001267676.1	1.06e-279	93.71	chloride channel protein CLC-b-like [Cucumis sativus] >AFJ15538.1 chloride chann...	[more]
XP_038885188.1	8.31e-272	91.01	chloride channel protein CLC-b isoform X2 [Benincasa hispida]	[more]
XP_008441347.1	1.67e-271	91.24	PREDICTED: chloride channel protein CLC-b [Cucumis melo] >KAA0056578.1 chloride ...	[more]
XP_022991119.1	4.02e-260	86.97	chloride channel protein CLC-b-like [Cucurbita maxima]	[more]
XP_022955252.1	4.02e-260	86.97	chloride channel protein CLC-b-like [Cucurbita moschata]	[more]

BLAST of Cucsat.G4825 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
I1Z8C8	5.13e-280	93.71	Chloride channel protein OS=Cucumis sativus OX=3659 GN=ClCa PE=2 SV=1	[more]
A0A5D3DLW5	8.09e-272	91.24	Chloride channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3B373	8.09e-272	91.24	Chloride channel protein OS=Cucumis melo OX=3656 GN=LOC103485488 PE=3 SV=1	[more]
A0A6J1JTX8	1.95e-260	86.97	chloride channel protein CLC-b-like OS=Cucurbita maxima OX=3661 GN=LOC111487811 ...	[more]
A0A6J1GT18	1.95e-260	86.97	chloride channel protein CLC-b-like OS=Cucurbita moschata OX=3662 GN=LOC11145726...	[more]

BLAST of Cucsat.G4825 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G27170.1	3.9e-177	72.85	chloride channel B	[more]
AT5G40890.1	9.5e-168	70.34	chloride channel A	[more]
AT5G40890.2	9.5e-168	70.34	chloride channel A	[more]
AT5G33280.1	2.6e-104	47.09	Voltage-gated chloride channel family protein	[more]
AT5G49890.1	5.9e-101	46.03	chloride channel C	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001807	Chloride channel, voltage gated	PRINTS	PR00762	CLCHANNEL	coord: 141..157 score: 47.18 coord: 109..129 score: 53.05 coord: 159..178 score: 47.9 coord: 197..211 score: 50.4
IPR001807	Chloride channel, voltage gated	PFAM	PF00654	Voltage_CLC	coord: 4..191 e-value: 2.5E-27 score: 96.1
IPR000644	CBS domain	SMART	SM00116	cbs_1	coord: 347..400 e-value: 2.5 score: 15.0 coord: 232..292 e-value: 0.1 score: 21.7
IPR000644	CBS domain	PFAM	PF00571	CBS	coord: 346..396 e-value: 0.001 score: 19.4
IPR000644	CBS domain	PROSITE	PS51371	CBS	coord: 344..407 score: 9.624166
None	No IPR available	GENE3D	3.10.580.10		coord: 230..397 e-value: 3.3E-6 score: 29.4
None	No IPR available	GENE3D	1.10.3080.10	Clc chloride channel	coord: 1..212 e-value: 1.2E-40 score: 141.7
None	No IPR available	PANTHER	PTHR11689:SF146	CHLORIDE CHANNEL PROTEIN	coord: 1..411
None	No IPR available	PANTHER	PTHR11689	CHLORIDE CHANNEL PROTEIN CLC FAMILY MEMBER	coord: 1..411
None	No IPR available	CDD	cd04591	CBS_pair_voltage-gated_CLC_euk_bac	coord: 230..398 e-value: 6.41892E-26 score: 99.1309
None	No IPR available	SUPERFAMILY	54631	CBS-domain pair	coord: 214..396
IPR014743	Chloride channel, core	SUPERFAMILY	81340	Clc chloride channel	coord: 2..212

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G4825.T10	Cucsat.G4825.T10	mRNA
Cucsat.G4825.T4	Cucsat.G4825.T4	mRNA
Cucsat.G4825.T8	Cucsat.G4825.T8	mRNA
Cucsat.G4825.T5	Cucsat.G4825.T5	mRNA
Cucsat.G4825.T6	Cucsat.G4825.T6	mRNA
Cucsat.G4825.T7	Cucsat.G4825.T7	mRNA
Cucsat.G4825.T1	Cucsat.G4825.T1	mRNA
Cucsat.G4825.T2	Cucsat.G4825.T2	mRNA
Cucsat.G4825.T3	Cucsat.G4825.T3	mRNA
Cucsat.G4825.T11	Cucsat.G4825.T11	mRNA
Cucsat.G4825.T9	Cucsat.G4825.T9	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006821	chloride transport
biological_process	GO:0015706	nitrate transport
biological_process	GO:1902600	proton transmembrane transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009705	plant-type vacuole membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0009671	nitrate:proton symporter activity
molecular_function	GO:0005247	voltage-gated chloride channel activity