Cucsat.G4639 (gene) Cucumber (B10) v3

Overview
NameCucsat.G4639
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionAB hydrolase-1 domain-containing protein
Locationctg1227: 1062918 .. 1064578 (+)
RNA-Seq ExpressionCucsat.G4639
SyntenyCucsat.G4639
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAGAAACGAAGGTAAATTAGTTGACAATGGAGTATAGCCACATATAGACAGTTCACCACATGCTTATCACGTTCAAAAGCCCATAATTGCCTTGCAATTTCTCAAAACAGAGTCCCCATTAATTCAAACTCTGATTTTGAGAAGATTCTTTCATGTTCTTCAAATGACAAAGTGTTTCTTCTTCAGTATCGTAGAGACCAAAAATTGGTTCCATCGTTATAGCTTCACCAAATCCGGTCTCCGATCCACCATTACAGATCTCAAAGACGGCACCACAGTTCATTGTTGGGTTCCCAAAAACCCAATTCACACCAAACCCAATCTCCTTTTAATCCATGGTATTGGAGCCAATGCACTTTGGCAATGGGGTGATTTCATTCCAGCTCTCATACCTTATTTCAATCTCTACATCCCTGATTTGATCTTCTTCGGCGACTCGTTTACCACTCAGCCAGACCGGACTGAGTGGTTTCAAGCTCAGTGTCTAATACGAGTCATGGAGGCTAACACGGTGGGGAAATTCAGTTTGGTGGGGCTTAGTTATGGAGGATTTGTTGGGTACAGCATCGCGGCGCTGCGGCCTGAAATGGTGGAGAGAGTAGTGATTTGTTGTTCTGGGGTTTGTGTGGAAGAGAAGGATTTTAAAGATGGGTTGTTGAAAGTTTCTGCTTTGGAGGATGCTACAGCCATTTTGGTTCCCCAAAAGCCAGAGAAGCTTAAACAGCTAGTGGGTTACTCCTTTTTCCGGCCGCCGCCGTTGCGGCTGATACCTTCTTGTTTGCTCAATGATTTCATTGAAGTAAGGCTTGTCGTAGTATTTGTGATTCTTCCATCACATCTTTTTTATTTGAAAATTTCCTTCCTTTTTGTGTGTTAATTTGACTAGTTTATCTTTGTGTTTGTGTGGCAGTCAATGTGTTTAGATCATATTGAGGAAAAGAGAGAGTTGATTCGAACTATTCCCAGAGGAAGAAAGCTTTCCGACCTTCCAAAGATTCAACAGGTGGGATTGCTATTCAACATAACTTATCCATATTAGCTTTAAACAGATCATTAATTACTTGTCTTCATTGCCATTCCATTCTACTCTCTGTTCTTCGTAATGTTCAAATAGCTTAGCATTTTTACACCCGAGTCTAAGTCCTTCGAATATTGTTAAAAATTTATTGATGCTATTAGACATTCTATTAGATACTTTTGAGATCGATTCAAACAGACAAAAAAACTCCAATCTCAAAAACTAAACTTGTAGTTTAACATTTTTCTGACTTGCTTGCAGCGGACGATGATAATGTGGGGAGAACATGATCAAGTATTTCCATTAGAATTGGGTCACAGGTTGAAAAGGTGAGTTAATCAAGCTCCTAAAAGCAAACAATGCATCAAATATTCTCATTTTGAACCAAATGATAAATGTGTTGGCCACTCCTTTGAAGTAGGCATTTGGGAGACAATGCTACGCTTGTTGTTATCAAGAATACAGGCCATGCCTTCAACTCAGAGGAGCCAAAGGAGTTTCTCAGCCATTTGATTTCATTCCTAGTTGATCCATAGCCTCCTCTCCTCATCAATCAATGAACGTCAAGCAGTTCAAATGCACAAACAACATGATCAAGAAACAGATAATGAGCAACGTCAAAAGCTGGAGTGATTTAATGATCGTTAATAGTACAATAACAAACAAGAGAAGAAATATACCTCAGCCATAGCTCAATTGGTTCATTCGTCCAAATCTAATGTTGAAAATACATTAAAACAATTAAATTTGTTATAGAAATTTCTGACATTTTCCATTTTGACCCTTTTGTTCCGAGTTCTAGATTCAGAAATCTTTAACCATAAAATCTGTGTTCCTAAGTACTCCTCAACCAACAAGACTTCAGGCATAATTTCTTGCACTATTATTTACTACAAGTCCTTCAATTTCTGGCAGCAATTCCATCTGATGACCAATGGGACCAAAGACATTGAATAATATGAAATAGTTAAACAATGAGTTCTACAATTACGA

Coding sequence (CDS)

ATGACAAAGTGTTTCTTCTTCAGTATCGTAGAGACCAAAAATTGGTTCCATCGTTATAGCTTCACCAAATCCGGTCTCCGATCCACCATTACAGATCTCAAAGACGGCACCACAGTTCATTGTTGGGTTCCCAAAAACCCAATTCACACCAAACCCAATCTCCTTTTAATCCATGGTATTGGAGCCAATGCACTTTGGCAATGGGGTGATTTCATTCCAGCTCTCATACCTTATTTCAATCTCTACATCCCTGATTTGATCTTCTTCGGCGACTCGTTTACCACTCAGCCAGACCGGACTGAGTGGTTTCAAGCTCAGTGTCTAATACGAGTCATGGAGGCTAACACGGTGGGGAAATTCAGTTTGGTGGGGCTTAGTTATGGAGGATTTGTTGGGTACAGCATCGCGGCGCTGCGGCCTGAAATGGTGGAGAGAGTAGTGATTTGTTGTTCTGGGGTTTGTGTGGAAGAGAAGGATTTTAAAGATGGGTTGTTGAAAGTTTCTGCTTTGGAGGATGCTACAGCCATTTTGGTTCCCCAAAAGCCAGAGAAGCTTAAACAGCTAGTGGGTTACTCCTTTTTCCGGCCGCCGCCGTTGCGGCTGATACCTTCTTGTTTGCTCAATGATTTCATTGAATCAATGTGTTTAGATCATATTGAGGAAAAGAGAGAGTTGATTCGAACTATTCCCAGAGGAAGAAAGCTTTCCGACCTTCCAAAGATTCAACAGCGGACGATGATAATGTGGGGAGAACATGATCAAGTATTTCCATTAGAATTGGGTCACAGGTTGAAAAGGCATTTGGGAGACAATGCTACGCTTGTTGTTATCAAGAATACAGGCCATGCCTTCAACTCAGAGGAGCCAAAGGAGTTTCTCAGCCATTTGATTTCATTCCTAGTTGATCCATAG

Protein sequence

MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQKPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPKIQQRTMIMWGEHDQVFPLELGHRLKRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFLVDP
Homology
BLAST of Cucsat.G4639 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.8e-11
Identity = 69/278 (24.82%), Postives = 120/278 (43.17%), Query Frame = 0

Query: 47  DGTTVHCWVPKNPIHTKPNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFT 106
           DG  V+    +NP   +  L+ +HG  ++A + +   IP L   +++   DL  FG S  
Sbjct: 13  DGVNVYYEHYQNP--GRQTLVCVHGFLSSA-FSFRKVIPLLRDKYDIIALDLPPFGQS-- 72

Query: 107 TQPDRTEWFQ----AQCLIRVMEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICC 166
            +  RT  +     A+ +I ++E   V +  LVG S GG +  S A  +PE+  +VV+ C
Sbjct: 73  -EKSRTFIYTYQNLAKLVIGILEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLC 132

Query: 167 SGVCVEEKDFKDGLLKVSALEDATAILVPQKPEKLKQLVGYSFFRPPPLRLI--PSCLLN 226
           S           G LK S         +P     +K+ +         L ++   S +  
Sbjct: 133 S----------SGYLKRSHPTIIFGTHIPYFHLYIKRWLSKEGVMKNLLNVVHDKSLIDE 192

Query: 227 DFIESMCLDHIEEK-----RELIRTIPRGRKLSDLPKIQQRTMIMWGEHDQVFPLELGHR 286
           + I+       +E+        IR      +   L K+ +  +++WGE D++ P+E+G R
Sbjct: 193 EMIDGYGRPFQDEQIFKAMTRFIRHREGDLEPEQLKKMNKPALLIWGEEDRIVPMEIGKR 252

Query: 287 LKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 314
           L +     N+ L  +  TGH    E P+    H+  F+
Sbjct: 253 LHADL--PNSVLYSLGQTGHLVPEERPELISEHIADFI 272

BLAST of Cucsat.G4639 vs. ExPASy Swiss-Prot
Match: P91143 (Abhydrolase domain-containing protein abhd-5.1 OS=Caenorhabditis elegans OX=6239 GN=abhd-5.1 PE=3 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 1.4e-08
Identity = 36/115 (31.30%), Postives = 59/115 (51.30%), Query Frame = 0

Query: 50  TVHCWVPKNPIHTKPNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDS----F 109
           TV+C    + + +K  ++LIHG GA     WG  I  L  + N+Y  DL  FG S    F
Sbjct: 58  TVNCENESSELKSKYPIVLIHGFGAGVA-LWGSAIKRLAQFQNVYAIDLPGFGRSSRTKF 117

Query: 110 TTQPDRTEWFQAQCLIRVMEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVI 161
           +T P+  E    + + +      + K +LVG S+GG++  S A   P+ +E +++
Sbjct: 118 STDPETAEKEMIEAIEQWRVKMNLEKMNLVGHSFGGYLSTSYALKYPKRIENLIL 171

BLAST of Cucsat.G4639 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.4e-08
Identity = 58/264 (21.97%), Postives = 111/264 (42.05%), Query Frame = 0

Query: 64  PNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRV 123
           P +LLIHGIG N+   W      L   F +  PDL+  G S   + D +    A  +  +
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 124 MEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALE 183
           +    + + ++VG S GG V    A   P++V+R+++  +G   ++ +    L  +    
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 184 DATAI----LVPQKPEKLKQLVGYSFFRPPPLRLIPSCL--LNDFIESMCLDH------- 243
           +A A+    LV    +   ++VG +         +P+ L  L+D  E             
Sbjct: 158 EAMALLRLPLVLPAVQIAGRIVGKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRTLRA 217

Query: 244 -IEEKRELIRTIPRGRKLSDLPKIQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVV 303
            ++ + +++  + R      +P       I+WG  D V P+   H   +   G  + L +
Sbjct: 218 VVDWRGQMVTMLDRCYLTEAIP-----VQIIWGTKDVVLPVRHAHMAHAAMPG--SQLEI 277

Query: 304 IKNTGHAFNSEEPKEFLSHLISFL 314
            + +GH    ++P  F+  +  F+
Sbjct: 278 FEGSGHFPFHDDPARFIDIVERFM 293

BLAST of Cucsat.G4639 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.4e-08
Identity = 58/264 (21.97%), Postives = 111/264 (42.05%), Query Frame = 0

Query: 64  PNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRV 123
           P +LLIHGIG N+   W      L   F +  PDL+  G S   + D +    A  +  +
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 124 MEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALE 183
           +    + + ++VG S GG V    A   P++V+R+++  +G   ++ +    L  +    
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 184 DATAI----LVPQKPEKLKQLVGYSFFRPPPLRLIPSCL--LNDFIESMCLDH------- 243
           +A A+    LV    +   ++VG +         +P+ L  L+D  E             
Sbjct: 158 EAMALLRLPLVLPAVQIAGRIVGKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRTLRA 217

Query: 244 -IEEKRELIRTIPRGRKLSDLPKIQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVV 303
            ++ + +++  + R      +P       I+WG  D V P+   H   +   G  + L +
Sbjct: 218 VVDWRGQMVTMLDRCYLTEAIP-----VQIIWGTKDVVLPVRHAHMAHAAMPG--SQLEI 277

Query: 304 IKNTGHAFNSEEPKEFLSHLISFL 314
            + +GH    ++P  F+  +  F+
Sbjct: 278 FEGSGHFPFHDDPARFIDIVERFM 293

BLAST of Cucsat.G4639 vs. ExPASy Swiss-Prot
Match: P0A573 (Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2734 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.4e-08
Identity = 58/264 (21.97%), Postives = 111/264 (42.05%), Query Frame = 0

Query: 64  PNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRV 123
           P +LLIHGIG N+   W      L   F +  PDL+  G S   + D +    A  +  +
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 124 MEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALE 183
           +    + + ++VG S GG V    A   P++V+R+++  +G   ++ +    L  +    
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 184 DATAI----LVPQKPEKLKQLVGYSFFRPPPLRLIPSCL--LNDFIESMCLDH------- 243
           +A A+    LV    +   ++VG +         +P+ L  L+D  E             
Sbjct: 158 EAMALLRLPLVLPAVQIAGRIVGKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRTLRA 217

Query: 244 -IEEKRELIRTIPRGRKLSDLPKIQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVV 303
            ++ + +++  + R      +P       I+WG  D V P+   H   +   G  + L +
Sbjct: 218 VVDWRGQMVTMLDRCYLTEAIP-----VQIIWGTKDVVLPVRHAHMAHAAMPG--SQLEI 277

Query: 304 IKNTGHAFNSEEPKEFLSHLISFL 314
            + +GH    ++P  F+  +  F+
Sbjct: 278 FEGSGHFPFHDDPARFIDIVERFM 293

BLAST of Cucsat.G4639 vs. NCBI nr
Match: XP_031737138.1 (uncharacterized protein LOC101222914 isoform X1 [Cucumis sativus])

HSP 1 Score: 637 bits (1644), Expect = 7.82e-231
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 72
           MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI
Sbjct: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60

Query: 73  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 132
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120

Query: 133 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 192
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180

Query: 193 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 252
           KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 253 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 312
           IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF
Sbjct: 241 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 300

Query: 313 LVDP 316
           LVDP
Sbjct: 301 LVDP 304

BLAST of Cucsat.G4639 vs. NCBI nr
Match: XP_004152639.1 (uncharacterized protein LOC101222914 isoform X2 [Cucumis sativus] >KGN62693.1 hypothetical protein Csa_022618 [Cucumis sativus])

HSP 1 Score: 631 bits (1628), Expect = 2.07e-228
Identity = 303/304 (99.67%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 72
           MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI
Sbjct: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60

Query: 73  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 132
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120

Query: 133 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 192
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180

Query: 193 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 252
           KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 253 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 312
           IQQRTMIMWGEHDQVFPLELGHRLK RHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF
Sbjct: 241 IQQRTMIMWGEHDQVFPLELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 300

Query: 313 LVDP 316
           LVDP
Sbjct: 301 LVDP 303

BLAST of Cucsat.G4639 vs. NCBI nr
Match: KAA0065149.1 (2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase [Cucumis melo var. makuwa])

HSP 1 Score: 614 bits (1584), Expect = 1.06e-221
Identity = 293/304 (96.38%), Postives = 299/304 (98.36%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 72
           MTKC FFSI ETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPI TKPNLLLIHGI
Sbjct: 1   MTKCLFFSITETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIRTKPNLLLIHGI 60

Query: 73  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 132
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEA+TVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEAHTVGKF 120

Query: 133 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 192
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVS LE+ATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 193 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 252
           KPEKLKQLVGYSFFRPPPLRL+PSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 253 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 312
           IQQRT+IMWGEHDQVFP+ELGHRLK RHLGDNATLVVIKNTGHAFNSEEPKEFL+HLISF
Sbjct: 241 IQQRTLIMWGEHDQVFPVELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPKEFLNHLISF 300

Query: 313 LVDP 316
           LVDP
Sbjct: 301 LVDP 303

BLAST of Cucsat.G4639 vs. NCBI nr
Match: XP_008444836.2 (PREDICTED: LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase [Cucumis melo])

HSP 1 Score: 612 bits (1577), Expect = 1.24e-220
Identity = 292/304 (96.05%), Postives = 298/304 (98.03%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 72
           MTKC FFSI ETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPI TKPNLLLIHGI
Sbjct: 1   MTKCLFFSITETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIRTKPNLLLIHGI 60

Query: 73  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 132
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEA+TVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEAHTVGKF 120

Query: 133 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 192
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVS LE+ATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 193 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 252
           KPEKLKQLVGYSF RPPPLRL+PSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFXRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 253 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 312
           IQQRT+IMWGEHDQVFP+ELGHRLK RHLGDNATLVVIKNTGHAFNSEEPKEFL+HLISF
Sbjct: 241 IQQRTLIMWGEHDQVFPVELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPKEFLNHLISF 300

Query: 313 LVDP 316
           LVDP
Sbjct: 301 LVDP 303

BLAST of Cucsat.G4639 vs. NCBI nr
Match: XP_038885546.1 (uncharacterized protein LOC120075885 [Benincasa hispida])

HSP 1 Score: 591 bits (1523), Expect = 2.11e-212
Identity = 279/303 (92.08%), Postives = 292/303 (96.37%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 72
           MT+CFFFSI ETKNW HRYSF KSGLRST+TDLKDGTTVHCWVP NPIHTKPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWCHRYSFIKSGLRSTVTDLKDGTTVHCWVPNNPIHTKPNLLLIHGI 60

Query: 73  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 132
           GANALWQWGDFIPAL+P+FNLYIPDLIFFGDSFTT+PDRTEWFQAQCLIRVMEAN+V K 
Sbjct: 61  GANALWQWGDFIPALVPHFNLYIPDLIFFGDSFTTRPDRTEWFQAQCLIRVMEANSVVKL 120

Query: 133 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 192
           SLVGLSYGGFVGYSIAALRPEMVE+VVICCSG+CVEEKDFKDGLLKVS LE+ATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVEKVVICCSGICVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 193 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 252
           KP+KLKQLVGYSFFRPPPLRL+PSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPDKLKQLVGYSFFRPPPLRLMPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 253 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 312
           I QRTMIMWGEHDQVFPLELGHRLK RHLG NATLVV+KN GHAFNSEEPKEFL+HLISF
Sbjct: 241 IHQRTMIMWGEHDQVFPLELGHRLK-RHLGANATLVVVKNAGHAFNSEEPKEFLNHLISF 300

Query: 313 LVD 315
           LVD
Sbjct: 301 LVD 302

BLAST of Cucsat.G4639 vs. ExPASy TrEMBL
Match: A0A0A0LLN7 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368940 PE=4 SV=1)

HSP 1 Score: 631 bits (1628), Expect = 1.00e-228
Identity = 303/304 (99.67%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 72
           MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI
Sbjct: 1   MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 60

Query: 73  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 132
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 120

Query: 133 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 192
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 180

Query: 193 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 252
           KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 253 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 312
           IQQRTMIMWGEHDQVFPLELGHRLK RHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF
Sbjct: 241 IQQRTMIMWGEHDQVFPLELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 300

Query: 313 LVDP 316
           LVDP
Sbjct: 301 LVDP 303

BLAST of Cucsat.G4639 vs. ExPASy TrEMBL
Match: A0A5A7VI51 (2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2, 4-dienoic acid hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004850 PE=4 SV=1)

HSP 1 Score: 614 bits (1584), Expect = 5.12e-222
Identity = 293/304 (96.38%), Postives = 299/304 (98.36%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 72
           MTKC FFSI ETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPI TKPNLLLIHGI
Sbjct: 1   MTKCLFFSITETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIRTKPNLLLIHGI 60

Query: 73  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 132
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEA+TVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEAHTVGKF 120

Query: 133 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 192
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVS LE+ATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 193 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 252
           KPEKLKQLVGYSFFRPPPLRL+PSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 253 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 312
           IQQRT+IMWGEHDQVFP+ELGHRLK RHLGDNATLVVIKNTGHAFNSEEPKEFL+HLISF
Sbjct: 241 IQQRTLIMWGEHDQVFPVELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPKEFLNHLISF 300

Query: 313 LVDP 316
           LVDP
Sbjct: 301 LVDP 303

BLAST of Cucsat.G4639 vs. ExPASy TrEMBL
Match: A0A1S3BC20 (LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase OS=Cucumis melo OX=3656 GN=LOC103488063 PE=4 SV=1)

HSP 1 Score: 612 bits (1577), Expect = 5.98e-221
Identity = 292/304 (96.05%), Postives = 298/304 (98.03%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 72
           MTKC FFSI ETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPI TKPNLLLIHGI
Sbjct: 1   MTKCLFFSITETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIRTKPNLLLIHGI 60

Query: 73  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 132
           GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEA+TVGKF
Sbjct: 61  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEAHTVGKF 120

Query: 133 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 192
           SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVS LE+ATAILVPQ
Sbjct: 121 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVPQ 180

Query: 193 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 252
           KPEKLKQLVGYSF RPPPLRL+PSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFXRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 240

Query: 253 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 312
           IQQRT+IMWGEHDQVFP+ELGHRLK RHLGDNATLVVIKNTGHAFNSEEPKEFL+HLISF
Sbjct: 241 IQQRTLIMWGEHDQVFPVELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPKEFLNHLISF 300

Query: 313 LVDP 316
           LVDP
Sbjct: 301 LVDP 303

BLAST of Cucsat.G4639 vs. ExPASy TrEMBL
Match: A0A6J1HDR2 (uncharacterized protein LOC111462585 OS=Cucurbita moschata OX=3662 GN=LOC111462585 PE=4 SV=1)

HSP 1 Score: 566 bits (1458), Expect = 8.22e-203
Identity = 260/302 (86.09%), Postives = 287/302 (95.03%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 72
           MT+CFFFSI ETKNW+HR+ FTK GLRSTITDL DGTTVHCWVPKNP+H+KPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWYHRHRFTKCGLRSTITDLNDGTTVHCWVPKNPLHSKPNLLLIHGI 60

Query: 73  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 132
           GANALWQWGD I  L+P+FNLY+PDLIFFGDSF+T PDRTEWFQAQCLIRVMEAN+VGK 
Sbjct: 61  GANALWQWGDSIATLVPHFNLYVPDLIFFGDSFSTNPDRTEWFQAQCLIRVMEANSVGKL 120

Query: 133 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 192
           SLVGLSYGGFVGYS+AAL P+MVE+VVICC+GVCVEEKDFKDGLL+VS LE+A AILVPQ
Sbjct: 121 SLVGLSYGGFVGYSMAALCPDMVEKVVICCAGVCVEEKDFKDGLLRVSDLEEAIAILVPQ 180

Query: 193 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 252
           KPEKLKQLVGYSFFRPPP+RL+PSCLLNDFIESMCLDHI+EKRELIRTIP+GRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPKGRKLSDLPK 240

Query: 253 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 312
           I QRTMI+WGEHD+VFPLELGHRLK RHLGDNATLV++KN GHAFNSEEPKEFL+HL+SF
Sbjct: 241 IHQRTMILWGEHDEVFPLELGHRLK-RHLGDNATLVIVKNAGHAFNSEEPKEFLNHLVSF 300

Query: 313 LV 314
           L+
Sbjct: 301 LL 301

BLAST of Cucsat.G4639 vs. ExPASy TrEMBL
Match: A0A6J1KAV5 (uncharacterized protein LOC111492191 OS=Cucurbita maxima OX=3661 GN=LOC111492191 PE=4 SV=1)

HSP 1 Score: 562 bits (1448), Expect = 2.75e-201
Identity = 259/302 (85.76%), Postives = 285/302 (94.37%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 72
           MT+CFFFSI ETKNW+HR+ FTK GLRSTITDL DGTTVHCWVPKNP+H+KPNLLLIHGI
Sbjct: 1   MTRCFFFSITETKNWYHRHRFTKCGLRSTITDLNDGTTVHCWVPKNPLHSKPNLLLIHGI 60

Query: 73  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 132
           GANALWQWGD I  L+P+FNLY+PDLIFFGDS TT PDRTEWFQAQCLIRVMEAN+VGK 
Sbjct: 61  GANALWQWGDSIATLVPHFNLYVPDLIFFGDSSTTNPDRTEWFQAQCLIRVMEANSVGKL 120

Query: 133 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 192
           SLVGLSYGGFVGYS+AAL P+MVE+VVICC+G+CVEEKDFKDGLL+VS LE+A AILVPQ
Sbjct: 121 SLVGLSYGGFVGYSMAALCPDMVEKVVICCAGICVEEKDFKDGLLRVSDLEEAIAILVPQ 180

Query: 193 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 252
           KPEKLKQLVGYSFFRPPP+RL+PSCLLNDFIESMCLDHI+EKRELIRTIP+GRKLSDLPK
Sbjct: 181 KPEKLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPKGRKLSDLPK 240

Query: 253 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 312
           I  RTMI+WGEHD++FPLELGHRLK RHLGDNATLV+IKN GHAFNSEEPKEFL+HLISF
Sbjct: 241 IHPRTMILWGEHDEIFPLELGHRLK-RHLGDNATLVIIKNAGHAFNSEEPKEFLNHLISF 300

Query: 313 LV 314
           L+
Sbjct: 301 LL 301

BLAST of Cucsat.G4639 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 374.4 bits (960), Expect = 9.0e-104
Identity = 173/313 (55.27%), Postives = 244/313 (77.96%), Query Frame = 0

Query: 8   FHVLQMTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDG-----TTVHCWVPKNPIHT 67
           F  +++ +CF F+   +++W  R SF  +GLRS  TDL  G     T +HCW+PK+P  +
Sbjct: 2   FDHMKLLRCFSFT--ASRDWLFRQSFANAGLRSVTTDLSHGNSIASTAMHCWIPKSPNRS 61

Query: 68  KPNLLLIHGIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIR 127
           KPNLLL+HG GANA+WQ+G+ + A    FN+Y+PDL+FFG S T++P+RTE FQA+CL+R
Sbjct: 62  KPNLLLLHGFGANAMWQYGEHLRAFTGRFNVYVPDLLFFGLSSTSEPNRTESFQARCLMR 121

Query: 128 VMEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSAL 187
           +MEA+ V + ++VG+SYGGFVGYS+AA  PE VE++V+CC+GVC+EEKD +DGL KV  L
Sbjct: 122 LMEAHGVQRMNIVGISYGGFVGYSLAAQFPENVEKLVLCCAGVCLEEKDMEDGLFKVPNL 181

Query: 188 EDATAILVPQKPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIP 247
           E+AT IL+PQ PEKLK+L+ +SF +  P++ +PS  L DFI+ MC + +EEKR+LI++I 
Sbjct: 182 EEATGILIPQTPEKLKELIRFSFVK--PIKGVPSFFLWDFIDVMCTEFVEEKRDLIKSIL 241

Query: 248 RGRKLSDLPKIQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEP 307
           + R+LSDLP+I+Q+++I+WGE DQ+FPLELG+RLK RH+G++A +VVIK  GHA N E+ 
Sbjct: 242 KDRRLSDLPRIKQKSLIIWGEEDQIFPLELGYRLK-RHIGESAEIVVIKKAGHAVNLEKS 301

Query: 308 KEFLSHLISFLVD 316
           KEF+ HL SFL+D
Sbjct: 302 KEFVKHLKSFLID 309

BLAST of Cucsat.G4639 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 359.4 bits (921), Expect = 3.0e-99
Identity = 174/306 (56.86%), Postives = 233/306 (76.14%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHT--KPNLLLIH 72
           MT C  FS+ E     ++  F +SGLR    DLKDGT V+ WV K    +  KPNLLLIH
Sbjct: 1   MTGC--FSLSEALERTYKSGFKRSGLRPVTIDLKDGTVVNFWVSKTKPESKPKPNLLLIH 60

Query: 73  GIGANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVG 132
           G+GA A+WQW D    L  YFNLYIPDL+FFG S TT+P+R++ FQAQ L+R +EA +V 
Sbjct: 61  GLGATAIWQWYDVARRLSRYFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVK 120

Query: 133 KFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILV 192
           KFSLVGLSYGGFVGY +A++  + VE+VVICC+ VCVEEKD K G+ KVS L++A+ ILV
Sbjct: 121 KFSLVGLSYGGFVGYRMASMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILV 180

Query: 193 PQKPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIE-SMCLDHIEEKRELIRTIPRGRKLSD 252
           P+  +KL++L+GY F++P   RL+P+CLL+DFIE ++  D++EEKRELI+ IP+ R +S+
Sbjct: 181 PESVKKLRELMGYIFYKPALARLVPTCLLHDFIEHALTRDNMEEKRELIKAIPKDRIISE 240

Query: 253 LPKIQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHL 312
           +PK++Q T+I+WGEHDQVFPLE+G RL+ +H+GDN  LV+IK TGH FN E+PK+F+  L
Sbjct: 241 IPKLKQPTLIIWGEHDQVFPLEMGKRLE-KHVGDNGKLVIIKRTGHIFNFEKPKKFIKLL 300

Query: 313 ISFLVD 316
            SFL++
Sbjct: 301 KSFLLE 303

BLAST of Cucsat.G4639 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 354.8 bits (909), Expect = 7.4e-98
Identity = 165/302 (54.64%), Postives = 224/302 (74.17%), Query Frame = 0

Query: 13  MTKCFFFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVPKNPIHTKPNLLLIHGI 72
           M  C  FS V ++N  ++YSF+++GLRS+ +DL DGT  HCW+P   IHTKP LLL+HGI
Sbjct: 1   MASC--FSYVSSRNKCYQYSFSRAGLRSSTSDLGDGTVFHCWIPLTHIHTKPTLLLLHGI 60

Query: 73  GANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKF 132
           GANA+WQW  FI   IP FN+Y+PDLIFFGDS+TT+PDR+E FQA C+++ M+A  V   
Sbjct: 61  GANAMWQWDRFIDRFIPRFNVYVPDLIFFGDSYTTRPDRSESFQATCVMKAMDAYGVRTM 120

Query: 133 SLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQ 192
           ++ GLSYGGFV YS+AA   E V+RVV+ C+GV +EEKD +DG+ KV + E+A A+L PQ
Sbjct: 121 TVAGLSYGGFVAYSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKVKSPEEAAAVLFPQ 180

Query: 193 KPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLSDLPK 252
            P  L++L+  SF++PP    IPSC   D+I  MC D+++E++EL+  + +GR+ ++LPK
Sbjct: 181 SPSMLRRLLQLSFYKPP--IWIPSCFAMDYIHVMCKDYLQERKELVEALHKGRRFANLPK 240

Query: 253 IQQRTMIMWGEHDQVFPLELGHRLKSRHLG-DNATLVVIKNTGHAFNSEEPKEFLSHLIS 312
           I Q T+++WGE DQVFP+EL HRLK R+LG D A LV++K TGHA N E+PKE   H+ S
Sbjct: 241 ITQPTLMIWGEEDQVFPVELAHRLK-RYLGEDRAQLVLLKKTGHAINEEKPKEMYKHMKS 297

Query: 313 FL 314
           FL
Sbjct: 301 FL 297

BLAST of Cucsat.G4639 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 205.3 bits (521), Expect = 7.2e-53
Identity = 113/304 (37.17%), Postives = 170/304 (55.92%), Query Frame = 0

Query: 18  FFSIVETKNWFHRYSFTKSGLRSTITDLKDGTTVHCWVP----KNPIHTKPNLLLIHGIG 77
           F S+        R  F+ +GL      +   TT+  W P     +    KP+LLL+HG G
Sbjct: 5   FLSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLLHGFG 64

Query: 78  ANALWQWGDFIPALIPYFNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKFS 137
            +A+WQW   +  L  +F LY+PDL+FFG S ++  +R+E FQA C+ ++ME   V +FS
Sbjct: 65  PSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEVERFS 124

Query: 138 LVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQK 197
           +VG SYGGFV Y++A + PE VE+VV+  SGV +   D  +  +  +       +++P  
Sbjct: 125 VVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSD-NEAFIARAKCHRIKEVMLPAS 184

Query: 198 PEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGR-KLSDLPK 257
              L++  G        L  +P  +LNDF + M  +  EEK EL+  +  G+   +++  
Sbjct: 185 ATDLRRFSG--MVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDKTNVSP 244

Query: 258 IQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISF 317
           IQQ  M++WGE DQVFPL++ H LK   LG  ATL VI+ T H   +E+ KEF   ++SF
Sbjct: 245 IQQDVMLIWGEQDQVFPLKMAHDLKEM-LGTKATLKVIQKTSHIPQTEKSKEFNGFVMSF 304

BLAST of Cucsat.G4639 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 185.3 bits (469), Expect = 7.8e-47
Identity = 102/285 (35.79%), Postives = 166/285 (58.25%), Query Frame = 0

Query: 36  SGLRSTITDLKDGTTVHCWVPKNPIH----TKPNLLLIHGIGANALWQWGDFIPALIP-Y 95
           +GL S    +   TT+H W P    H     +P +LL+HG G +++WQW   + A  P  
Sbjct: 24  AGLTSQTLSIDSETTIHFWGPPPLDHRSDDDRPVMLLLHGFGPSSMWQWRRQMQAFSPSA 83

Query: 96  FNLYIPDLIFFGDSFTTQPDRTEWFQAQCLIRVMEANTVGKFSLVGLSYGGFVGYSIAAL 155
           F +Y PDL+FFGDS ++  +RTE FQA+C+ ++M    +GK+++ G SYGGFV Y +A +
Sbjct: 84  FRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIGKYNVAGTSYGGFVAYHMAKM 143

Query: 156 RPEMVERVVICCSGVCVEEKDFKDGLLKVSALEDATAILVPQKPEKLKQLVGY-SFFRPP 215
            PE VE+VVI  SG+ + + D  + LL+ S  E    +++P    + + L+   S +R  
Sbjct: 144 WPEKVEKVVIASSGINMRKCD-GESLLQRSNCECIEKVMLPSTATEFRTLMALASSWR-- 203

Query: 216 PLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPRGRKLS-DLPKIQQRTMIMWGEHDQVF 275
            +R+ P  L ND I ++   + +EK EL++ +  GR  + ++  + Q  +I+WG+ DQ+F
Sbjct: 204 LVRMFPDALWNDVINNLYKKNRKEKIELLKGVTFGRSENLNIDSLSQEVLIVWGDKDQIF 263

Query: 276 PLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPKEFLSHLISFL 314
           P+++ + LK   LGD   L +I NT H    E  +EF + ++ FL
Sbjct: 264 PVKMAYELK-EILGDKTKLEIIDNTSHVPQIECAQEFNNIVLRFL 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O052351.8e-1124.82Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yu... [more]
P911431.4e-0831.30Abhydrolase domain-containing protein abhd-5.1 OS=Caenorhabditis elegans OX=6239... [more]
P9WNH21.4e-0821.97Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / ... [more]
P9WNH31.4e-0821.97Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 ... [more]
P0A5731.4e-0821.97Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2... [more]
Match NameE-valueIdentityDescription
XP_031737138.17.82e-231100.00uncharacterized protein LOC101222914 isoform X1 [Cucumis sativus][more]
XP_004152639.12.07e-22899.67uncharacterized protein LOC101222914 isoform X2 [Cucumis sativus] >KGN62693.1 hy... [more]
KAA0065149.11.06e-22196.382-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase [Cucumis melo ... [more]
XP_008444836.21.24e-22096.05PREDICTED: LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dieno... [more]
XP_038885546.12.11e-21292.08uncharacterized protein LOC120075885 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0LLN71.00e-22899.67AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368... [more]
A0A5A7VI515.12e-22296.382-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2, 4-dienoic acid hydrolase OS=Cucumis me... [more]
A0A1S3BC205.98e-22196.05LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hyd... [more]
A0A6J1HDR28.22e-20386.09uncharacterized protein LOC111462585 OS=Cucurbita moschata OX=3662 GN=LOC1114625... [more]
A0A6J1KAV52.75e-20185.76uncharacterized protein LOC111492191 OS=Cucurbita maxima OX=3661 GN=LOC111492191... [more]
Match NameE-valueIdentityDescription
AT5G09430.19.0e-10455.27alpha/beta-Hydrolases superfamily protein [more]
AT1G78210.13.0e-9956.86alpha/beta-Hydrolases superfamily protein [more]
AT4G39955.17.4e-9854.64alpha/beta-Hydrolases superfamily protein [more]
AT5G21950.17.2e-5337.17alpha/beta-Hydrolases superfamily protein [more]
AT4G33180.17.8e-4735.79alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 245..259
score: 42.53
coord: 136..149
score: 30.3
coord: 78..93
score: 27.59
coord: 122..135
score: 37.93
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 52..151
e-value: 3.2E-10
score: 40.1
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 16..266
e-value: 1.7E-34
score: 121.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 27..265
NoneNo IPR availablePANTHERPTHR43139SI:DKEY-122A22.2coord: 6..266
NoneNo IPR availablePANTHERPTHR43139:SF54ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 6..266

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G4639.T2Cucsat.G4639.T2mRNA
Cucsat.G4639.T1Cucsat.G4639.T1mRNA
Cucsat.G4639.T3Cucsat.G4639.T3mRNA
Cucsat.G4639.T5Cucsat.G4639.T5mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity