Cucsat.G4307 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G4307
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	chloroplast processing peptidase-like
Location	ctg1170: 192320 .. 195792 (+)
RNA-Seq Expression	Cucsat.G4307
Synteny	Cucsat.G4307

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAAATTTGAAGAATGAAGAACGTAGTCTCATTTGAAGAACAAAATCACTGTATCAACTCATTTTTTTCTCCACTCCTCACTTTCCCAGAAACTTACACACGAGGTCACTTCCTCCTCTTTCTCTTCCATTTTCTCCAATTTCAATCCAAATTCCATCGGACCTCCTCCATTCTCCACACACATTTCCTCTCTTTCCTCCCATATCTGGAATGACCTTTTTGAGGCCCTTTCTGTTTTACTCTTCGCTGCGATGGATGACTTTTCACAGTTGGGGTTTTCCCAGATGGCCTAGCTTTGACGCTTTCCTTAGATTTCTTGTCCTTTTGATCTTGTGGTCTATGCTTTTGGAGATTCATTCCATTCCTTCCTCATCAATGTATCCAACTCTTCGCGTCGGCGATCGTATTCTTGTCGAGACGGTAATATTTTTCAAGCTGGGTTTTGTATTTATCTGTCGAATTATGTGCTTAATTTTTTTTTTTTTTAAATTTCAGACTTCCACTAGGTTTTAGGGTATTTTTGTGCATGTGATGTGAAAATGGATTGAGATTTTAAGCGTGGTTATGATGAAGCTCGATCGATCGACTGAATCTTCAAAATTATGTGTCAGTTATATCATATTCGAAGAGTATCGGTGAATCATGTGTATTAAAGTATTAACTATGCATTAGTTTACAAAAACGTTGGTAGTTTCTTGTTAGTTCTATTATTATATAAAGAAAAAAGAATCAAACATATAGAAAACAGTATAATCATGAGCAAGACAACGGAATGTGATTTCAGTAATTTAGACAGAAATTATTTTCGTCTTTATAAGTTTTACAAGAGCCTGTCATTTTTTGAACGCGAAAACTCGTTTTTGAATAGTTTGGTCTAAGCTGAGGTAACATAGCTAGGTGAACAAAGTAGAGGGACTTTTGGGGGAATAAGTTTAAACTCTGTTGTATCGATGACCTATGACATTTTGTAACGTCTAAACATTGTCCTATGACATTAGTTGTGAGACACTCACAGATAACAAAGAAAAAAAAAGGATGGTTAATGTGATTTACATTGACTGTTCTGAAAATTTTCATGTGATCTCTGCTCAAACTAATATCAATGTCCTGGTTGCATTTTCTTCAGGCCTCTTATCTTATCAGAAATCCTTCTATAAATGACATTATAACCTTCCGAGACCCGACACAGGTTAGTTGACAGATGACTAATCTGCATGTCTTTTACTTGAACAGTTTTTCCTAACAGGATCGTGTTGGAATTATGGTGTGCTATAGCAATCTGGACTCAGAGAAGAGAACATTGTTGTAAAGAGAGTTGTTGCCAAAGCCGGAGACACAGTTGAGGTATGGAAGATTGTTGTTATGTTTCTATTGAAAATAACTAGTGATATAAATGTCTAAGACATCAAATTATTATTTATATTTATTTTGATGTAATCTTAGTATTTTCCTATCTTATCCTTTTTATGTTCTATTATTCAGTAGGGGGTATTTTTGTCATTTTACTATGATAAATGACGTGGCATTAGGATTTTCATTAGGCAATTGTGGCCTTTGTGCTCAGTTTAGCTCAGGGAGCTTCTTGAATTTGGTAAATATTGAATTGCATGCCAAGAACATTCGAGTGGCATTAATCGTCCTATCTTGTAGAGCGTTCAAAGCTTGAGCGCAACTTATTCTTGTTCTTGTCTCAAGAGCTTTGATCTTAAGATAATTTGTATCTAACACAATCCTTAGGGTTGAAATCATATAGATGGAGGGTTCTAATGTATGAGAATTAGGAATTCTGATAGCAAAAGAGTTCCTAATTCTAAGTCACTAGTGTTTTCGTATCAATGGGAGTGACCACTTTTTGTGCACTAATAAATGTACACTTATAGCTACCCTTAAATGGTGACTCTAGTAATAAGAAATTGGGTTTGTTGATGTATCTAAGGTCCAAGCTTTGCCTATACAGAAGATTGTTGCCTGAAATAATTTCGATAAGCTACGAACATAATGCAAGAAATGGGACTCCCCAACAGTCCAGTTATGAAAGAAAATGTATATTGGCAGTTTATGTTTAACTAATTTTCCATTTTATGAGTCACTGACTGCTAAAAACGCCATATCAATTAGCAGGAGAAGAACTTCTAGTGTTGAAGTCAAAGAAACTAACCATTGATGTGGTTTTTATCCAACACCTTTCAGGTTTGTCAGGGGTGCCTCTATGTCAATGGGGTTGCTAAGGATGAAAGCTTCTTACTGGAGCGGCCAATGTACACCACAAAGTCAACTGTGAGTTTTGTTTGAAACTTGACTTTCGTTTTCATTTTTACTGATTCCTCCTACAATTTTCTATTAGAGAGATAAGGCTTTTAAAGCACGTCCTTAGAGATTTTATAGATGAACCGAAATCGTTAAGCCGTCAAGGTGATTTCATAATGCAAAACGAACCCATATTCTTTAGAAATCATCTTCAGACTGTCATTGATTTTCGCCACAGCTAAGTCATTGATATTTTGATCAGTTGTTGGAATCAGACTTGAATAATACTGGTTTCCTACGGAATGTGACTTGTAGTTTCAAGTTTGTAGTTAATTTCACTTGTTTGAAACTTGCAGCAAATTCCAGAAGGCCATGTTTACGTGTTGGGCGACAACCGCAACAACAGCTATGACTCCCATATTTGGTTAGTGTTCCCAACTTCATGTTATATTATGCATTTTTGCTGGTTGTCGTTGGTTCTGATTGGGGTCAGGGTGGTTCGGTGTCAGTTTGGAAATATTCCAAATCGACAAATTTAAAAAAATAAAAAATAAAAAATAAAAAATAGAACCGATAGACCTTTACTCCTCAACAGTCAAGGTCGGTTCTAACATTTACTAAACTAACCGTAGTTGGTTTGGTGGCAATGTGGTCGAAAAACCGACTCCAATTGACCTATGCTCACCCATAGTTCTCATGCGTATAGTTTGCCTCTCCAAAGAAAAAGTCTTTCAACATTTTTGAATTGTATCATTGTTCTTGGAATTGAATGCTTCCCAAGTTTTTATTTCTCTCTCTCAACTTCATCTACGATGTTCTTATAATTTTTGTCACTCACTTGAACAGGGGACCTCTGCCCGTAAAAAACATCATAGGGAGATACCTGTGTTCATGCCATAAACCAACAAGTAAATGATACATTCAACAAATTGAAATGAGGTCAGAAACATTATTCTATTTGTATGTGATAAATTATTTTAGTTCCAAATCTCATGTAGGATTAGGCTTGTTTGGCATGGATGGATATGAATACTAAAGAAACATTTTATTCATTCTTAGGTGTGGTTTATAAATTTGTGTATTAGGTGTGATGGAAGTTAGAGTTATTTTTTTTATTCGTTATCTTTAACAACCATGCCCTTTTATTTGTTTATAATATTCTTTTTAAATGTACAATTAAGGATGAATGATAAAAATATGTTGTTTCTTTAGAATCTCTGCT

Coding sequence (CDS)

ATGACCTTTTTGAGGCCCTTTCTGTTTTACTCTTCGCTGCGATGGATGACTTTTCACAGTTGGGGTTTTCCCAGATGGCCTAGCTTTGACGCTTTCCTTAGATTTCTTGTCCTTTTGATCTTGTGGTCTATGCTTTTGGAGATTCATTCCATTCCTTCCTCATCAATGTATCCAACTCTTCGCGTCGGCGATCGTATTCTTGTCGAGACGGCCTCTTATCTTATCAGAAATCCTTCTATAAATGACATTATAACCTTCCGAGACCCGACACAGCAATCTGGACTCAGAGAAGAGAACATTGTTGTAAAGAGAGTTGTTGCCAAAGCCGGAGACACAGTTGAGGTTTGTCAGGGGTGCCTCTATGTCAATGGGGTTGCTAAGGATGAAAGCTTCTTACTGGAGCGGCCAATGTACACCACAAAGTCAACTCAAATTCCAGAAGGCCATGTTTACGTGTTGGGCGACAACCGCAACAACAGCTATGACTCCCATATTTGGGGACCTCTGCCCGTAAAAAACATCATAGGGAGATACCTGTGTTCATGCCATAAACCAACAAGTAAATGA

Protein sequence

MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTLRVGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTVEVCQGCLYVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLCSCHKPTSK

Homology

[Close]

BLAST of Cucsat.G4307 vs. ExPASy Swiss-Prot
Match: Q8H0W1 (Chloroplast processing peptidase OS=Arabidopsis thaliana OX=3702 GN=PLSP1 PE=2 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 2.2e-38
Identity = 81/163 (49.69%), Postives = 103/163 (63.19%), Query Frame = 0


Query: 23  FPRWPSFDA------FLRFLVLLILWSMLLEIHSIPSSSMYPTLRVGDRILVETASYLIR 82
           FP W  F +      F+   V L     + E   IPS SMYPT  VGDR++ E  SY  R
Sbjct: 104 FPEWLDFTSDDAQTVFVAIAVSLAFRYFIAEPRYIPSLSMYPTFDVGDRLVAEKVSYYFR 163

Query: 83  NPSINDIITFRDP--TQQSGLREENIVVKRVVAKAGDTVEVCQGCLYVNGVAKDESFLLE 142
            P  NDI+ F+ P   Q+ G  + ++ +KR+VAK GD VEV  G L VNGVA++E F+LE
Sbjct: 164 KPCANDIVIFKSPPVLQEVGYTDADVFIKRIVAKEGDLVEVHNGKLMVNGVARNEKFILE 223

Query: 143 RPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGR 178
            P Y     ++PE  V+V+GDNRNNSYDSH+WGPLP+KNIIGR
Sbjct: 224 PPGYEMTPIRVPENSVFVMGDNRNNSYDSHVWGPLPLKNIIGR 266

[Close]

BLAST of Cucsat.G4307 vs. ExPASy Swiss-Prot
Match: Q9M9Z2 (Probable thylakoidal processing peptidase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPP2 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.0e-34
Identity = 75/149 (50.34%), Postives = 96/149 (64.43%), Query Frame = 0


Query: 31  AFLRFLVLLILWSMLLEIHSIPSSSMYPTLRVGDRILVETASYLIRNPSINDIITFRDP- 90
           AF    V L+  S L E  SIPS+SM PTL VGDR++ E  SY  R P ++DI+ F+ P 
Sbjct: 190 AFTAVTVSLLFRSALAEPKSIPSTSMLPTLDVGDRVIAEKVSYFFRKPEVSDIVIFKAPP 249

Query: 91  -TQQSGLREENIVVKRVVAKAGDTVEVCQGCLYVNGVAKDESFLLERPMYTTKSTQIPEG 150
              + G    ++ +KR+VA  GD VEVC G L VN   + E F+LE   Y  +   +PEG
Sbjct: 250 ILVEHGYSCADVFIKRIVASEGDWVEVCDGKLLVNDTVQAEDFVLEPIDYEMEPMFVPEG 309

Query: 151 HVYVLGDNRNNSYDSHIWGPLPVKNIIGR 178
           +V+VLGDNRN S+DSH WGPLP+KNIIGR
Sbjct: 310 YVFVLGDNRNKSFDSHNWGPLPIKNIIGR 338

[Close]

BLAST of Cucsat.G4307 vs. ExPASy Swiss-Prot
Match: O04348 (Thylakoidal processing peptidase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPP1 PE=2 SV=2)

HSP 1 Score: 145.2 bits (365), Expect = 7.4e-34
Identity = 70/152 (46.05%), Postives = 98/152 (64.47%), Query Frame = 0


Query: 31  AFLRFLVLLILWSMLLEIHSIPSSSMYPTLRVGDRILVETASYLIRNPSINDIITFRDPT 90
           AF    V ++  S L E  SIPS+SMYPTL  GDR++ E  SY  R P ++DI+ F+ P 
Sbjct: 160 AFTAVTVSILFRSALAEPKSIPSTSMYPTLDKGDRVMAEKVSYFFRKPEVSDIVIFKAPP 219

Query: 91  -----QQSGLREENIVVKRVVAKAGDTVEVCQGCLYVNGVAKDESFLLERPMYTTKSTQI 150
                 + G    ++ +KR+VA  GD VEV  G L+VN + ++E F+LE   Y  +   +
Sbjct: 220 ILLEYPEYGYSSNDVFIKRIVASEGDWVEVRDGKLFVNDIVQEEDFVLEPMSYEMEPMFV 279

Query: 151 PEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGR 178
           P+G+V+VLGDNRN S+DSH WGPLP++NI+GR
Sbjct: 280 PKGYVFVLGDNRNKSFDSHNWGPLPIENIVGR 311

[Close]

BLAST of Cucsat.G4307 vs. ExPASy Swiss-Prot
Match: P72660 (Probable signal peptidase I-1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=lepB1 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.2e-28
Identity = 66/146 (45.21%), Postives = 87/146 (59.59%), Query Frame = 0


Query: 36  LVLLILWSMLLEIHSIPSSSMYPTLRVGDRILVETASYLIRNPSINDIITFRDP--TQQS 95
           ++ L+L   + E   IPS SM PTL  GDR++VE  SY    P + DII F  P   Q  
Sbjct: 25  VLALLLRFFVAEPRYIPSDSMLPTLEQGDRLVVEKVSYHFHPPQVGDIIVFHPPELLQVQ 84

Query: 96  GLREENIVVKRVVAKAGDTVEVCQGCLYVNGVAKDESFLLERPMYTTKSTQIPEGHVYVL 155
           G       +KRV+A  G TVEV  G +Y +G    E ++LE P Y   + ++P+G V+V+
Sbjct: 85  GYDLGQAFIKRVIALPGQTVEVNNGIVYRDGQPLQEEYILEPPQYNLPAVRVPDGQVFVM 144

Query: 156 GDNRNNSYDSHIWGPLPVKNIIGRYL 180
           GDNRNNS DSH+WG LP +NIIG  L
Sbjct: 145 GDNRNNSNDSHVWGFLPQQNIIGHAL 170

[Close]

BLAST of Cucsat.G4307 vs. ExPASy Swiss-Prot
Match: P73157 (Probable signal peptidase I-2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=lepB2 PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 7.5e-26
Identity = 54/135 (40.00%), Postives = 80/135 (59.26%), Query Frame = 0


Query: 43  SMLLEIHSIPSSSMYPTLRVGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVV 102
           + + E   IPSSSM PTL++ DR+++E  SY +R+P   +I+ F            +  +
Sbjct: 40  TFVAEARYIPSSSMEPTLQINDRLIIEKISYRLRDPERGEIVVFNPTDALKAKNFHDAFI 99

Query: 103 KRVVAKAGDTVEVCQGCLYVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYD 162
           KR++   GD V V QG +YVNG   DE+++   P Y     ++P+    VLGDNRNNSYD
Sbjct: 100 KRIIGLPGDEVRVSQGNVYVNGKMLDENYIAAPPAYEYGPVKVPDDQYLVLGDNRNNSYD 159

Query: 163 SHIWGPLPVKNIIGR 178
           SH WG +P + ++GR
Sbjct: 160 SHYWGFVPREKLLGR 174

[Close]

BLAST of Cucsat.G4307 vs. NCBI nr
Match: XP_004149428.1 (chloroplast processing peptidase [Cucumis sativus] >KGN47696.1 hypothetical protein Csa_018302 [Cucumis sativus])

HSP 1 Score: 385 bits (989), Expect = 4.57e-135
Identity = 187/188 (99.47%), Postives = 187/188 (99.47%), Query Frame = 0


Query: 1   MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL 60
           MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL
Sbjct: 1   MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL 60

Query: 61  RVGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTVEVCQGCL 120
           RVGDRILVETASYLIRNPSINDIITFRDPTQQS LREENIVVKRVVAKAGDTVEVCQGCL
Sbjct: 61  RVGDRILVETASYLIRNPSINDIITFRDPTQQSALREENIVVKRVVAKAGDTVEVCQGCL 120

Query: 121 YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC 180
           YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC
Sbjct: 121 YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC 180

Query: 181 SCHKPTSK 188
           SCHKPTSK
Sbjct: 181 SCHKPTSK 188

[Close]

BLAST of Cucsat.G4307 vs. NCBI nr
Match: XP_008466751.1 (PREDICTED: chloroplast processing peptidase-like [Cucumis melo])

HSP 1 Score: 357 bits (917), Expect = 4.36e-124
Identity = 171/188 (90.96%), Postives = 178/188 (94.68%), Query Frame = 0


Query: 1   MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL 60
           M+FLRPFLFYSSLRWM FHSWGFPRWP FD FLRF+VLLILWSMLLEIHSIPSSSMYPTL
Sbjct: 1   MSFLRPFLFYSSLRWMIFHSWGFPRWPGFDGFLRFIVLLILWSMLLEIHSIPSSSMYPTL 60

Query: 61  RVGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTVEVCQGCL 120
           RVGDRILVE ASY IRNPSINDIITF+DPTQQSG REENIVVKRVVAKAGDTVEV QGCL
Sbjct: 61  RVGDRILVEKASYFIRNPSINDIITFQDPTQQSGCREENIVVKRVVAKAGDTVEVFQGCL 120

Query: 121 YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC 180
           YVNG+A+DESFL ERP YTTKSTQIPEGH+YVLGDNRNNSYDSHIWGPLP+KNIIGR LC
Sbjct: 121 YVNGIAQDESFLRERPTYTTKSTQIPEGHIYVLGDNRNNSYDSHIWGPLPIKNIIGRCLC 180

Query: 181 SCHKPTSK 188
           SCHKPTSK
Sbjct: 181 SCHKPTSK 188

[Close]

BLAST of Cucsat.G4307 vs. NCBI nr
Match: XP_038885468.1 (chloroplast processing peptidase-like [Benincasa hispida])

HSP 1 Score: 346 bits (888), Expect = 1.11e-119
Identity = 167/187 (89.30%), Postives = 174/187 (93.05%), Query Frame = 0


Query: 1   MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL 60
           M+FLRPFL YSSLRWM   SWGFPR P FD  LRFLV+LILWSMLLEIHSIPSSSMYPTL
Sbjct: 1   MSFLRPFLLYSSLRWMNCQSWGFPRLPGFDGLLRFLVVLILWSMLLEIHSIPSSSMYPTL 60

Query: 61  RVGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTVEVCQGCL 120
           RVGDRILVE ASY IRNPSI+DIITFRDPTQQSG REENIVVKRVVAKAGDTVEVCQGCL
Sbjct: 61  RVGDRILVEKASYYIRNPSIDDIITFRDPTQQSGRREENIVVKRVVAKAGDTVEVCQGCL 120

Query: 121 YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC 180
           YVNG+A+DESFL ERP YTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLP+KNI+GRYLC
Sbjct: 121 YVNGIAQDESFLAERPTYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPIKNILGRYLC 180

Query: 181 SCHKPTS 187
           SCHKPTS
Sbjct: 181 SCHKPTS 187

[Close]

BLAST of Cucsat.G4307 vs. NCBI nr
Match: XP_022141359.1 (chloroplast processing peptidase-like isoform X1 [Momordica charantia])

HSP 1 Score: 330 bits (845), Expect = 4.42e-113
Identity = 155/186 (83.33%), Postives = 169/186 (90.86%), Query Frame = 0


Query: 3   FLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTLRV 62
           FLRPF+ YSSLRWMT  SWGFPRWP FD   RFLV+LILWSMLLEIH IPSSSMYPTLRV
Sbjct: 5   FLRPFILYSSLRWMTCQSWGFPRWPGFDGLFRFLVVLILWSMLLEIHYIPSSSMYPTLRV 64

Query: 63  GDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTVEVCQGCLYV 122
           GDRILVE ASY IRNP+I+DIITFRDPTQQSG REENIVVKRVVA +GD VEVC+GCLYV
Sbjct: 65  GDRILVEKASYYIRNPAIDDIITFRDPTQQSGRREENIVVKRVVATSGDMVEVCEGCLYV 124

Query: 123 NGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLCSC 182
           NG+A+ E+FL ++P YTTKSTQ+PEGHVYVLGDNRNNSYDSHIWGPLP+KNI+GRYL SC
Sbjct: 125 NGIAQKENFLADQPTYTTKSTQVPEGHVYVLGDNRNNSYDSHIWGPLPIKNIVGRYLWSC 184

Query: 183 HKPTSK 188
           HKPTSK
Sbjct: 185 HKPTSK 190

[Close]

BLAST of Cucsat.G4307 vs. NCBI nr
Match: XP_022991150.1 (chloroplast processing peptidase-like [Cucurbita maxima])

HSP 1 Score: 329 bits (843), Expect = 8.32e-113
Identity = 155/188 (82.45%), Postives = 169/188 (89.89%), Query Frame = 0


Query: 1   MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL 60
           M+FLRPF+ YS+LRWMT  SWGFPRWP FD  LR LV+LILWSM LEIH IPSSSMYPTL
Sbjct: 1   MSFLRPFMLYSTLRWMTCSSWGFPRWPGFDGLLRCLVVLILWSMYLEIHFIPSSSMYPTL 60

Query: 61  RVGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTVEVCQGCL 120
           R+GDRILVE  SY IRNPSI+DIITFRDPTQQSG REEN+VVKRVVAKAGD VEVC+GCL
Sbjct: 61  RIGDRILVEKVSYYIRNPSIDDIITFRDPTQQSGRREENLVVKRVVAKAGDMVEVCEGCL 120

Query: 121 YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC 180
           YVNG+A+DE FL ERP YTTKSTQ+P+GHVYVLGDNRNNSYDSHIWGPLP+KNIIGRYL 
Sbjct: 121 YVNGIAQDERFLAERPTYTTKSTQVPKGHVYVLGDNRNNSYDSHIWGPLPIKNIIGRYLW 180

Query: 181 SCHKPTSK 188
           SCH PT+K
Sbjct: 181 SCHNPTTK 188

[Close]

BLAST of Cucsat.G4307 vs. ExPASy TrEMBL
Match: A0A0A0KIU5 (Peptidase_S26 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G382350 PE=4 SV=1)

HSP 1 Score: 385 bits (989), Expect = 2.21e-135
Identity = 187/188 (99.47%), Postives = 187/188 (99.47%), Query Frame = 0


Query: 1   MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL 60
           MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL
Sbjct: 1   MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL 60

Query: 61  RVGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTVEVCQGCL 120
           RVGDRILVETASYLIRNPSINDIITFRDPTQQS LREENIVVKRVVAKAGDTVEVCQGCL
Sbjct: 61  RVGDRILVETASYLIRNPSINDIITFRDPTQQSALREENIVVKRVVAKAGDTVEVCQGCL 120

Query: 121 YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC 180
           YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC
Sbjct: 121 YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC 180

Query: 181 SCHKPTSK 188
           SCHKPTSK
Sbjct: 181 SCHKPTSK 188

[Close]

BLAST of Cucsat.G4307 vs. ExPASy TrEMBL
Match: A0A1S3CRY6 (chloroplast processing peptidase-like OS=Cucumis melo OX=3656 GN=LOC103504090 PE=4 SV=1)

HSP 1 Score: 357 bits (917), Expect = 2.11e-124
Identity = 171/188 (90.96%), Postives = 178/188 (94.68%), Query Frame = 0


Query: 1   MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL 60
           M+FLRPFLFYSSLRWM FHSWGFPRWP FD FLRF+VLLILWSMLLEIHSIPSSSMYPTL
Sbjct: 1   MSFLRPFLFYSSLRWMIFHSWGFPRWPGFDGFLRFIVLLILWSMLLEIHSIPSSSMYPTL 60

Query: 61  RVGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTVEVCQGCL 120
           RVGDRILVE ASY IRNPSINDIITF+DPTQQSG REENIVVKRVVAKAGDTVEV QGCL
Sbjct: 61  RVGDRILVEKASYFIRNPSINDIITFQDPTQQSGCREENIVVKRVVAKAGDTVEVFQGCL 120

Query: 121 YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC 180
           YVNG+A+DESFL ERP YTTKSTQIPEGH+YVLGDNRNNSYDSHIWGPLP+KNIIGR LC
Sbjct: 121 YVNGIAQDESFLRERPTYTTKSTQIPEGHIYVLGDNRNNSYDSHIWGPLPIKNIIGRCLC 180

Query: 181 SCHKPTSK 188
           SCHKPTSK
Sbjct: 181 SCHKPTSK 188

[Close]

BLAST of Cucsat.G4307 vs. ExPASy TrEMBL
Match: A0A6J1CIF2 (chloroplast processing peptidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011778 PE=4 SV=1)

HSP 1 Score: 330 bits (845), Expect = 2.14e-113
Identity = 155/186 (83.33%), Postives = 169/186 (90.86%), Query Frame = 0


Query: 3   FLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTLRV 62
           FLRPF+ YSSLRWMT  SWGFPRWP FD   RFLV+LILWSMLLEIH IPSSSMYPTLRV
Sbjct: 5   FLRPFILYSSLRWMTCQSWGFPRWPGFDGLFRFLVVLILWSMLLEIHYIPSSSMYPTLRV 64

Query: 63  GDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTVEVCQGCLYV 122
           GDRILVE ASY IRNP+I+DIITFRDPTQQSG REENIVVKRVVA +GD VEVC+GCLYV
Sbjct: 65  GDRILVEKASYYIRNPAIDDIITFRDPTQQSGRREENIVVKRVVATSGDMVEVCEGCLYV 124

Query: 123 NGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLCSC 182
           NG+A+ E+FL ++P YTTKSTQ+PEGHVYVLGDNRNNSYDSHIWGPLP+KNI+GRYL SC
Sbjct: 125 NGIAQKENFLADQPTYTTKSTQVPEGHVYVLGDNRNNSYDSHIWGPLPIKNIVGRYLWSC 184

Query: 183 HKPTSK 188
           HKPTSK
Sbjct: 185 HKPTSK 190

[Close]

BLAST of Cucsat.G4307 vs. ExPASy TrEMBL
Match: A0A6J1JS29 (chloroplast processing peptidase-like OS=Cucurbita maxima OX=3661 GN=LOC111487833 PE=4 SV=1)

HSP 1 Score: 329 bits (843), Expect = 4.03e-113
Identity = 155/188 (82.45%), Postives = 169/188 (89.89%), Query Frame = 0


Query: 1   MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL 60
           M+FLRPF+ YS+LRWMT  SWGFPRWP FD  LR LV+LILWSM LEIH IPSSSMYPTL
Sbjct: 1   MSFLRPFMLYSTLRWMTCSSWGFPRWPGFDGLLRCLVVLILWSMYLEIHFIPSSSMYPTL 60

Query: 61  RVGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTVEVCQGCL 120
           R+GDRILVE  SY IRNPSI+DIITFRDPTQQSG REEN+VVKRVVAKAGD VEVC+GCL
Sbjct: 61  RIGDRILVEKVSYYIRNPSIDDIITFRDPTQQSGRREENLVVKRVVAKAGDMVEVCEGCL 120

Query: 121 YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC 180
           YVNG+A+DE FL ERP YTTKSTQ+P+GHVYVLGDNRNNSYDSHIWGPLP+KNIIGRYL 
Sbjct: 121 YVNGIAQDERFLAERPTYTTKSTQVPKGHVYVLGDNRNNSYDSHIWGPLPIKNIIGRYLW 180

Query: 181 SCHKPTSK 188
           SCH PT+K
Sbjct: 181 SCHNPTTK 188

[Close]

BLAST of Cucsat.G4307 vs. ExPASy TrEMBL
Match: A0A6J1GPV1 (chloroplast processing peptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111456402 PE=4 SV=1)

HSP 1 Score: 328 bits (842), Expect = 5.72e-113
Identity = 156/188 (82.98%), Postives = 169/188 (89.89%), Query Frame = 0


Query: 1   MTFLRPFLFYSSLRWMTFHSWGFPRWPSFDAFLRFLVLLILWSMLLEIHSIPSSSMYPTL 60
           M+FLRPF+ YS+LRWMT  SWGFPRWP FD  LR LV+LILWSM LEIH IPSSSMYPTL
Sbjct: 1   MSFLRPFMLYSTLRWMTCLSWGFPRWPGFDGLLRCLVVLILWSMYLEIHFIPSSSMYPTL 60

Query: 61  RVGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTVEVCQGCL 120
           R+GDRILVE  SY IRNPSI+DIITFRDPTQQSG REEN+VVKRVVAKAGD VEVC+GCL
Sbjct: 61  RIGDRILVEKVSYYIRNPSIDDIITFRDPTQQSGRREENLVVKRVVAKAGDMVEVCEGCL 120

Query: 121 YVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGRYLC 180
           YVNGVA+DE FL ERP YTTKSTQ+P+GHVYVLGDNRNNSYDSHIWGPLP+KNIIGRYL 
Sbjct: 121 YVNGVAQDERFLAERPTYTTKSTQVPKGHVYVLGDNRNNSYDSHIWGPLPIKNIIGRYLW 180

Query: 181 SCHKPTSK 188
           SCH PT+K
Sbjct: 181 SCHNPTTK 188

[Close]

BLAST of Cucsat.G4307 vs. TAIR 10
Match: AT3G24590.1 (plastidic type i signal peptidase 1 )

HSP 1 Score: 160.2 bits (404), Expect = 1.6e-39
Identity = 81/163 (49.69%), Postives = 103/163 (63.19%), Query Frame = 0


Query: 23  FPRWPSFDA------FLRFLVLLILWSMLLEIHSIPSSSMYPTLRVGDRILVETASYLIR 82
           FP W  F +      F+   V L     + E   IPS SMYPT  VGDR++ E  SY  R
Sbjct: 104 FPEWLDFTSDDAQTVFVAIAVSLAFRYFIAEPRYIPSLSMYPTFDVGDRLVAEKVSYYFR 163

Query: 83  NPSINDIITFRDP--TQQSGLREENIVVKRVVAKAGDTVEVCQGCLYVNGVAKDESFLLE 142
            P  NDI+ F+ P   Q+ G  + ++ +KR+VAK GD VEV  G L VNGVA++E F+LE
Sbjct: 164 KPCANDIVIFKSPPVLQEVGYTDADVFIKRIVAKEGDLVEVHNGKLMVNGVARNEKFILE 223

Query: 143 RPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGR 178
            P Y     ++PE  V+V+GDNRNNSYDSH+WGPLP+KNIIGR
Sbjct: 224 PPGYEMTPIRVPENSVFVMGDNRNNSYDSHVWGPLPLKNIIGR 266

[Close]

BLAST of Cucsat.G4307 vs. TAIR 10
Match: AT1G06870.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 147.1 bits (370), Expect = 1.4e-35
Identity = 75/149 (50.34%), Postives = 96/149 (64.43%), Query Frame = 0


Query: 31  AFLRFLVLLILWSMLLEIHSIPSSSMYPTLRVGDRILVETASYLIRNPSINDIITFRDP- 90
           AF    V L+  S L E  SIPS+SM PTL VGDR++ E  SY  R P ++DI+ F+ P 
Sbjct: 190 AFTAVTVSLLFRSALAEPKSIPSTSMLPTLDVGDRVIAEKVSYFFRKPEVSDIVIFKAPP 249

Query: 91  -TQQSGLREENIVVKRVVAKAGDTVEVCQGCLYVNGVAKDESFLLERPMYTTKSTQIPEG 150
              + G    ++ +KR+VA  GD VEVC G L VN   + E F+LE   Y  +   +PEG
Sbjct: 250 ILVEHGYSCADVFIKRIVASEGDWVEVCDGKLLVNDTVQAEDFVLEPIDYEMEPMFVPEG 309

Query: 151 HVYVLGDNRNNSYDSHIWGPLPVKNIIGR 178
           +V+VLGDNRN S+DSH WGPLP+KNIIGR
Sbjct: 310 YVFVLGDNRNKSFDSHNWGPLPIKNIIGR 338

[Close]

BLAST of Cucsat.G4307 vs. TAIR 10
Match: AT2G30440.1 (thylakoid processing peptide )

HSP 1 Score: 145.2 bits (365), Expect = 5.3e-35
Identity = 70/152 (46.05%), Postives = 98/152 (64.47%), Query Frame = 0


Query: 31  AFLRFLVLLILWSMLLEIHSIPSSSMYPTLRVGDRILVETASYLIRNPSINDIITFRDPT 90
           AF    V ++  S L E  SIPS+SMYPTL  GDR++ E  SY  R P ++DI+ F+ P 
Sbjct: 160 AFTAVTVSILFRSALAEPKSIPSTSMYPTLDKGDRVMAEKVSYFFRKPEVSDIVIFKAPP 219

Query: 91  -----QQSGLREENIVVKRVVAKAGDTVEVCQGCLYVNGVAKDESFLLERPMYTTKSTQI 150
                 + G    ++ +KR+VA  GD VEV  G L+VN + ++E F+LE   Y  +   +
Sbjct: 220 ILLEYPEYGYSSNDVFIKRIVASEGDWVEVRDGKLFVNDIVQEEDFVLEPMSYEMEPMFV 279

Query: 151 PEGHVYVLGDNRNNSYDSHIWGPLPVKNIIGR 178
           P+G+V+VLGDNRN S+DSH WGPLP++NI+GR
Sbjct: 280 PKGYVFVLGDNRNKSFDSHNWGPLPIENIVGR 311

[Close]

BLAST of Cucsat.G4307 vs. TAIR 10
Match: AT1G23465.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.3e-09
Identity = 43/126 (34.13%), Postives = 60/126 (47.62%), Query Frame = 0


Query: 55  SMYPTLR-VGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTV 114
           SM PTL   G+ +L E  S   + PS  DI+  R P   +        +KRVV   GD +
Sbjct: 47  SMIPTLHPSGNMLLAERISKRYQKPSRGDIVVIRSPENPN-----KTPIKRVVGVEGDCI 106

Query: 115 EVCQGCLYVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKN 174
                   ++ V  DES          ++  +P+GHV+V GD  +NS DS  +GP+P   
Sbjct: 107 S-----FVIDPVKSDES----------QTIVVPKGHVFVQGDYTHNSRDSRNFGPVPYGL 152

Query: 175 IIGRYL 180
           I GR L
Sbjct: 167 IQGRVL 152

[Close]

BLAST of Cucsat.G4307 vs. TAIR 10
Match: AT1G29960.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.6e-07
Identity = 40/126 (31.75%), Postives = 58/126 (46.03%), Query Frame = 0


Query: 55  SMYPTLR-VGDRILVETASYLIRNPSINDIITFRDPTQQSGLREENIVVKRVVAKAGDTV 114
           SM PTL   G+ +L E  S   + PS  DI+  R P   +        +KRV+   GD +
Sbjct: 47  SMTPTLHPSGNVLLAERISKRYQKPSRGDIVVIRSPENPN-----KTPIKRVIGIEGDCI 106

Query: 115 EVCQGCLYVNGVAKDESFLLERPMYTTKSTQIPEGHVYVLGDNRNNSYDSHIWGPLPVKN 174
                   ++    DES          ++  +P+GHV+V GD  +NS DS  +G +P   
Sbjct: 107 S-----FVIDSRKSDES----------QTIVVPKGHVFVQGDYTHNSRDSRNFGTVPYGL 152

Query: 175 IIGRYL 180
           I GR L
Sbjct: 167 IQGRVL 152

The following BLAST results are available for this feature:

BLAST of Cucsat.G4307 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H0W1	2.2e-38	49.69	Chloroplast processing peptidase OS=Arabidopsis thaliana OX=3702 GN=PLSP1 PE=2 S...	[more]
Q9M9Z2	2.0e-34	50.34	Probable thylakoidal processing peptidase 2, chloroplastic OS=Arabidopsis thalia...	[more]
O04348	7.4e-34	46.05	Thylakoidal processing peptidase 1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
P72660	1.2e-28	45.21	Probable signal peptidase I-1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX...	[more]
P73157	7.5e-26	40.00	Probable signal peptidase I-2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX...	[more]

BLAST of Cucsat.G4307 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149428.1	4.57e-135	99.47	chloroplast processing peptidase [Cucumis sativus] >KGN47696.1 hypothetical prot...	[more]
XP_008466751.1	4.36e-124	90.96	PREDICTED: chloroplast processing peptidase-like [Cucumis melo]	[more]
XP_038885468.1	1.11e-119	89.30	chloroplast processing peptidase-like [Benincasa hispida]	[more]
XP_022141359.1	4.42e-113	83.33	chloroplast processing peptidase-like isoform X1 [Momordica charantia]	[more]
XP_022991150.1	8.32e-113	82.45	chloroplast processing peptidase-like [Cucurbita maxima]	[more]

BLAST of Cucsat.G4307 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIU5	2.21e-135	99.47	Peptidase_S26 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G3823...	[more]
A0A1S3CRY6	2.11e-124	90.96	chloroplast processing peptidase-like OS=Cucumis melo OX=3656 GN=LOC103504090 PE...	[more]
A0A6J1CIF2	2.14e-113	83.33	chloroplast processing peptidase-like isoform X1 OS=Momordica charantia OX=3673 ...	[more]
A0A6J1JS29	4.03e-113	82.45	chloroplast processing peptidase-like OS=Cucurbita maxima OX=3661 GN=LOC11148783...	[more]
A0A6J1GPV1	5.72e-113	82.98	chloroplast processing peptidase-like OS=Cucurbita moschata OX=3662 GN=LOC111456...	[more]

BLAST of Cucsat.G4307 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24590.1	1.6e-39	49.69	plastidic type i signal peptidase 1	[more]
AT1G06870.1	1.4e-35	50.34	Peptidase S24/S26A/S26B/S26C family protein	[more]
AT2G30440.1	5.3e-35	46.05	thylakoid processing peptide	[more]
AT1G23465.1	1.3e-09	34.13	Peptidase S24/S26A/S26B/S26C family protein	[more]
AT1G29960.1	1.6e-07	31.75	Peptidase S24/S26A/S26B/S26C family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000223	Peptidase S26A, signal peptidase I	PRINTS	PR00727	LEADERPTASE	coord: 145..164 score: 54.17 coord: 44..60 score: 43.98 coord: 101..113 score: 51.28
IPR000223	Peptidase S26A, signal peptidase I	TIGRFAM	TIGR02227	TIGR02227	coord: 34..179 e-value: 1.3E-36 score: 123.8
IPR000223	Peptidase S26A, signal peptidase I	PANTHER	PTHR43390	SIGNAL PEPTIDASE I	coord: 5..187
IPR019533	Peptidase S26	PFAM	PF10502	Peptidase_S26	coord: 32..178 e-value: 3.4E-37 score: 128.0
IPR019533	Peptidase S26	CDD	cd06530	S26_SPase_I	coord: 51..177 e-value: 5.38398E-26 score: 93.4191
None	No IPR available	GENE3D	2.10.109.10	Umud Fragment, subunit A	coord: 53..184 e-value: 3.4E-34 score: 119.5
None	No IPR available	PANTHER	PTHR43390:SF10	CHLOROPLAST PROCESSING PEPTIDASE-LIKE	coord: 5..187
IPR019756	Peptidase S26A, signal peptidase I, serine active site	PROSITE	PS00501	SPASE_I_1	coord: 53..60
IPR019758	Peptidase S26A, signal peptidase I, conserved site	PROSITE	PS00761	SPASE_I_3	coord: 150..163
IPR036286	LexA/Signal peptidase-like superfamily	SUPERFAMILY	51306	LexA/Signal peptidase	coord: 44..179

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G4307.T2	Cucsat.G4307.T2	mRNA
Cucsat.G4307.T6	Cucsat.G4307.T6	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006465	signal peptide processing
biological_process	GO:0010027	thylakoid membrane organization
biological_process	GO:0006508	proteolysis
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0005887	integral component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity