Cucsat.G4251 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G4251
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	E3 ubiquitin ligase BIG BROTHER-related-like
Location	ctg1170: 107261 .. 109687 (-)
RNA-Seq Expression	Cucsat.G4251
Synteny	Cucsat.G4251

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TATATCCTAAACCATGGCTCTTGCATAGCATGAATGTTCTCTAAGATTTTGATCATTTTTAATGGAAGAGCAAGAGGATAATAAGCAGCAGCAGCAGCAGCAACCACAGCTTCCCCAAAGGATTTCTCTTGCTTATGTTGAACAGGTGAACACAGATTTGATCATGGCCTTGGCAATGCAGCAGCAGGAGCACGAGATGGCCTATACGACGCTGGAGACCATTGCAAGTGATAGTGAAGAAGACGAAAATTCAGATTCCAACAGCAACAATGGTCTCGATACAAATGCTACCTCACAAAGGCAAGAACTTGTATCCCGGTGGGCTTTTTTGGAAGACGATGAGGAGACTACGGATGGAAATGATGATATGGAAGAAGATGAAGATGGGGATTTTGAGGGTTTTGATCTGGATGAGCTAACTTATGAGGAGTTGATTGCATTAGGGGAGTTCATTGGGGAAGAAAAGAGGGGCCTACCGATAAACGAAATACCTTCATGCTTGCACTCGAGCAAGTTCCAAACTATCGAAAACAGAAGTGGGATAGATCGGTGTGTGATTTGCCAAGTTGAATATGATGATGGTGAAGAATTGGCTGCTCTTCCTTGTGAGCATCCGTATCATTCAGAATGTATAGGCGAATGGCTTCAAATCAAAAGGGTTTGTCCAATATGTGGCACAGAAGTTTCATCCCCCAATGGTTCTAAAAATGAATAATCTCCAAACAAATTGGTGGGCTAATTGCAAAATGGGGGAAAAGGTCAGATCAATGTTCGTAATTTGTTCATTTAATTGAAAGGAAATGTTAAGAACTTCACAAAAAAGATAGGAAGGCTTCTTTATATTTTTATTGTTTTTAAAACCCAACATCTCATTCTAAAGGTAGCCTCTTTAGGTTCATTATGTTGGAAATTATTTGTATTAAGATTGTTGTGATTAACTGATACTTCTCGTTAAATTATTTACCTCATCAAATTCTTAGGAACAAAATCTAGGTAACAGATATACTTCATGTAATTATTTTAGATGAAGCAAAATGTGTGAGGAAAAAGATCTGAATAAATAATTTGAGCTGTATATAAATTAGGAATTAGGGAGATACAAAAGAATTCACAATTTTGTTGCTTACTTACTAAACTACACAAATTTATTGTTTATAAATTTATGTCAAAACCTTTCCATGGTTGTCAAAACCTTTCCATGGTTGTTTTGAAAAAACAAAAGGAAAAAGAAAGAAAAACCCAAACTTTGAAGATTAAAACGAAAACTTCCAAGTTTAGGGTATAATTAAAATCAACTCCTAGCTTACGGCTATTTGTATTTTGTGATAATTTAATCATCTTTTTTAAAACAAAAAAGATACAAGTAATAAACATTTCAGAAGAATTAAGATTAAAAAGCATTTTTGTTCCTTGAAAAGCAACAATTTAATCTATCAAACTTAGATGGTATTTATACTTTTAAATTTAAATTATTTAAATCCTATACAATTTATAAAGATTTTGTTTCTATTGAAAAAAAAACATCAAAATAAAGTTGTTTATTAGGTAATGAATTAATTTTATTTAATCACACTAATATTTTTCACCATAAAAACTAAAGCATTAACTGTTATTTATTAAATTTTAGAGACTATCAAAATTTAAAAGTATAAAAAACTAAATTGTTTTTATTTTCAATCAAATTTAAAAGTATAGAGACTAATTTCACAAATTAATTTTTAGACAGGTCATCATGGAATGAACTTTCTACTTTTTTTTTTATCTCCTCTTTTTAGTTCGGAGACTAAAGTTATTTTTCATAAAAATAAAGATTTAAATTTGTTAGTTTCACCAAAGTAGACACTCTTTTTCAAGCCTAGTAGACTCATTTAAGTAGAATCAGCTAATTACAAATGCACAAGAAGGAATCATACTATAATTTTTCTTGCTACTCTTTTATATATATATATATATATATATATATATATATATATATATATTAAGAATTATCTTAGTGCAGTAATATTGAGCAGGAATAATATTAAAAAATGGTCTATGTATTGCCTAAATTAAGACCGTAATTTATTTAAAGTTTTACATGATAAAACTAGGAGCCCAACAAGAAATCGAACAGCTGATTTGCTATGATAAAAAAACCTATTTACTTTTTCCTTTTACATTCATAAATGATGAGTAATTACAAAACCAACAAAACCAAACCCATCTTGTTCCTAAATACAACTCCTAAGTTTATGATTAATAAATTAATAGCCATATTGACTGTTAAAGAAAGTTACTTTCCCTTTAATTCAACATTATGCATTTCTTATTACTAAAACACCATTTTCCCCCTTCTTCTAGGACTCTTCATTAAACCACAACTCGTGATAAGCTTATATATAAACAGGAAACATGACCAGTTATTCTCACATTACATTCTGATTTCTG

Coding sequence (CDS)

ATGGAAGAGCAAGAGGATAATAAGCAGCAGCAGCAGCAGCAACCACAGCTTCCCCAAAGGATTTCTCTTGCTTATGTTGAACAGGTGAACACAGATTTGATCATGGCCTTGGCAATGCAGCAGCAGGAGCACGAGATGGCCTATACGACGCTGGAGACCATTGCAAGTGATAGTGAAGAAGACGAAAATTCAGATTCCAACAGCAACAATGGTCTCGATACAAATGCTACCTCACAAAGGCAAGAACTTGTATCCCGGTGGGCTTTTTTGGAAGACGATGAGGAGACTACGGATGGAAATGATGATATGGAAGAAGATGAAGATGGGGATTTTGAGGGTTTTGATCTGGATGAGCTAACTTATGAGGAGTTGATTGCATTAGGGGAGTTCATTGGGGAAGAAAAGAGGGGCCTACCGATAAACGAAATACCTTCATGCTTGCACTCGAGCAAGTTCCAAACTATCGAAAACAGAAGTGGGATAGATCGGTGTGTGATTTGCCAAGTTGAATATGATGATGGTGAAGAATTGGCTGCTCTTCCTTGTGAGCATCCGTATCATTCAGAATGTATAGGCGAATGGCTTCAAATCAAAAGGGTTTGTCCAATATGTGGCACAGAAGTTTCATCCCCCAATGGTTCTAAAAATGAATAA

Protein sequence

MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASDSEEDENSDSNSNNGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELAALPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE

Homology

[Close]

BLAST of Cucsat.G4251 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.5e-22
Identity = 80/258 (31.01%), Postives = 121/258 (46.90%), Query Frame = 0


Query: 20  RISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASD----------SEEDENSD---- 79
           R+    + Q++ DL +A  +Q+QE      T+ +  SD           +EDE  D    
Sbjct: 85  RVPFTNLSQIDADLALARTLQEQERAYMMLTMNSEISDYGSWETGSYVYDEDEFDDPENE 144

Query: 80  --SNSNNGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGF--------- 139
              +  +  +T+   Q   L       EDD+E  DGN D+EE    D E +         
Sbjct: 145 DEDDDEDEYETDDDPQEDGLDVNVHANEDDQE-DDGNSDIEEVAYTDDEAYARALQEAEE 204

Query: 140 -----------------------------------DLDELTYEELIALGEFIGEEKRGLP 199
                                              D DEL+YEEL+ALG+ +G E RGL 
Sbjct: 205 RDMAARLSALSGLANRVVEDLEDESHTSQDAWDEMDPDELSYEELLALGDIVGTESRGLS 264

Query: 200 INEIPSCLHSSKFQTIENRSGI-DRCVICQVEYDDGEELAALPCEHPYHSECIGEWLQIK 217
            + I S L S +++  +N++G  + CVIC+++Y+D E+L  LPC+H YHSECI  WL+I 
Sbjct: 265 ADTIAS-LPSKRYKEGDNQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKIN 324

[Close]

BLAST of Cucsat.G4251 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.2e-19
Identity = 53/143 (37.06%), Postives = 79/143 (55.24%), Query Frame = 0


Query: 75  NATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDF------EGFDLDELTYEELIALG 134
           N     +  V+R   + D +E ++  + +  + D D       +  D D +TYEEL+ LG
Sbjct: 100 NVADDPENTVARSVQIGDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELG 159

Query: 135 EFIGEEKRGLPINEIPSCLHSSKFQ---TIENRSGIDRCVICQVEYDDGEELAALPCEHP 194
           E +G E RGL   E+   L + K++       +   +RCVICQ++Y  GE    LPC+H 
Sbjct: 160 EAVGTESRGLS-QELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHV 219

Query: 195 YHSECIGEWLQIKRVCPICGTEV 209
           YHSECI +WL I +VCP+C +EV
Sbjct: 220 YHSECISKWLSINKVCPVCNSEV 241

[Close]

BLAST of Cucsat.G4251 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.5e-17
Identity = 42/98 (42.86%), Postives = 58/98 (59.18%), Query Frame = 0


Query: 115 DLDELTYEELIALGEFIGEEKRGLPINEIPSCLH-----SSKFQTIENRSGIDRCVICQV 174
           D+D ++YEEL+ALGE IG+   GL    I   +      SS   ++E    I+ C ICQ 
Sbjct: 602 DVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQE 661

Query: 175 EYDDGEELAALPCEHPYHSECIGEWLQIKRVCPICGTE 208
           EY +G+ L  L C H +H +CI +W+ IK +CPIC TE
Sbjct: 662 EYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

[Close]

BLAST of Cucsat.G4251 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 2.1e-16
Identity = 38/92 (41.30%), Postives = 53/92 (57.61%), Query Frame = 0


Query: 115 DLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDR--CVICQVEYD 174
           D+D ++YEEL+AL E IG    GL   E+   L   KF +    + ++   C ICQ EY 
Sbjct: 569 DIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQEEYV 628

Query: 175 DGEELAALPCEHPYHSECIGEWLQIKRVCPIC 205
           DG++L  L C H +H  C+ +WL +K  CPIC
Sbjct: 629 DGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

[Close]

BLAST of Cucsat.G4251 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.7e-16
Identity = 38/93 (40.86%), Postives = 59/93 (63.44%), Query Frame = 0


Query: 115 DLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQT-IENRSGIDRCVICQVEYDD 174
           D+D ++YEEL+AL E IG+   G+    I + L   K+++  ++    + C +CQ EY +
Sbjct: 587 DVDNMSYEELLALEERIGDVCTGVNEETISNRLKQRKYKSNTKSPQDAEPCCVCQEEYTE 646

Query: 175 GEELAALPCEHPYHSECIGEWLQIKRVCPICGT 207
           GE++  L C H +HS+CI EWL+ K +CPIC T
Sbjct: 647 GEDMGTLECGHEFHSQCIKEWLKQKNLCPICKT 679

[Close]

BLAST of Cucsat.G4251 vs. NCBI nr
Match: XP_004149804.1 (E3 ubiquitin ligase BIG BROTHER-related [Cucumis sativus] >KGN47704.1 hypothetical protein Csa_018241 [Cucumis sativus])

HSP 1 Score: 420 bits (1080), Expect = 5.60e-148
Identity = 216/217 (99.54%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASDSEE 60
           MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHE+AYTTLETIASDSEE
Sbjct: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHELAYTTLETIASDSEE 60

Query: 61  DENSDSNSNNGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGFDLDELT 120
           DENSDSNSNNGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGFDLDELT
Sbjct: 61  DENSDSNSNNGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGFDLDELT 120

Query: 121 YEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELAAL 180
           YEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELAAL
Sbjct: 121 YEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELAAL 180

Query: 181 PCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 217
           PCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE
Sbjct: 181 PCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 217

[Close]

BLAST of Cucsat.G4251 vs. NCBI nr
Match: XP_008466738.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER-related-like [Cucumis melo])

HSP 1 Score: 367 bits (941), Expect = 1.27e-126
Identity = 196/230 (85.22%), Postives = 208/230 (90.43%), Query Frame = 0


Query: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASDSEE 60
           MEEQEDN +QQQQQ  LPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIAS+SEE
Sbjct: 1   MEEQEDNNKQQQQQ--LPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASESEE 60

Query: 61  DENSDSNSNNGLDTNATSQRQELVSRWAFLEDDEE---TTDG----------NDDMEEDE 120
           DE+SDSNSNNGL+TNA SQR+EL+ RWAF +++E      DG          N+DMEEDE
Sbjct: 61  DESSDSNSNNGLNTNAASQREELICRWAFPDENEYMEGNEDGDYEGSDMDELNEDMEEDE 120

Query: 121 DGDFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVIC 180
           DGDFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIEN+SGIDRCVIC
Sbjct: 121 DGDFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENKSGIDRCVIC 180

Query: 181 QVEYDDGEELAALPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 217
           QVEYDDGEELAALPC+HPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE
Sbjct: 181 QVEYDDGEELAALPCQHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 228

[Close]

BLAST of Cucsat.G4251 vs. NCBI nr
Match: XP_038886321.1 (E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida] >XP_038886322.1 E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida] >XP_038886323.1 E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida])

HSP 1 Score: 365 bits (936), Expect = 3.89e-126
Identity = 189/216 (87.50%), Postives = 200/216 (92.59%), Query Frame = 0


Query: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASDSEE 60
           MEEQED +Q       LPQRISLA+VEQVN+DLIMALAMQQQEHEMAYT LETI SDS+E
Sbjct: 1   MEEQEDKQQ-------LPQRISLAHVEQVNSDLIMALAMQQQEHEMAYTMLETIESDSDE 60

Query: 61  DENSDSNSNNGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGFDLDELT 120
           DENSDSNSN+GL+TNA+SQRQEL+SRWAFLEDDEET D N+DMEEDEDGDFE FDLDELT
Sbjct: 61  DENSDSNSNHGLNTNASSQRQELLSRWAFLEDDEETADENEDMEEDEDGDFEDFDLDELT 120

Query: 121 YEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELAAL 180
           YEELIALGEFIG EKRGLPINEIPSCLH SKFQTIENRSGIDRCVICQVEYD+GEELAAL
Sbjct: 121 YEELIALGEFIGTEKRGLPINEIPSCLHPSKFQTIENRSGIDRCVICQVEYDEGEELAAL 180

Query: 181 PCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKN 216
           PCEHPYHSECIG+WLQIKRVCPICGTEVSSP GSKN
Sbjct: 181 PCEHPYHSECIGKWLQIKRVCPICGTEVSSPKGSKN 209

[Close]

BLAST of Cucsat.G4251 vs. NCBI nr
Match: KAA0055464.1 (E3 ubiquitin ligase BIG BROTHER-related-like [Cucumis melo var. makuwa] >TYK08946.1 E3 ubiquitin ligase BIG BROTHER-related-like [Cucumis melo var. makuwa])

HSP 1 Score: 363 bits (933), Expect = 6.43e-122
Identity = 194/228 (85.09%), Postives = 206/228 (90.35%), Query Frame = 0


Query: 3   EQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASDSEEDE 62
           EQEDN +QQQQQ  LPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIAS+SEEDE
Sbjct: 241 EQEDNNKQQQQQ--LPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASESEEDE 300

Query: 63  NSDSNSNNGLDTNATSQRQELVSRWAFLEDDEE---TTDG----------NDDMEEDEDG 122
           +SDSNSNNGL+TNA SQR+EL+ RWAF +++E      DG          N+DMEEDEDG
Sbjct: 301 SSDSNSNNGLNTNAASQREELICRWAFPDENEYMEGNEDGDYEGSDMDELNEDMEEDEDG 360

Query: 123 DFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQV 182
           DFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIEN+SGIDRCVICQV
Sbjct: 361 DFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENKSGIDRCVICQV 420

Query: 183 EYDDGEELAALPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 217
           EYDDGEELAALPC+HPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE
Sbjct: 421 EYDDGEELAALPCQHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 466

[Close]

BLAST of Cucsat.G4251 vs. NCBI nr
Match: XP_022954880.1 (E3 ubiquitin ligase BIG BROTHER-related [Cucurbita moschata])

HSP 1 Score: 319 bits (817), Expect = 5.39e-108
Identity = 174/218 (79.82%), Postives = 184/218 (84.40%), Query Frame = 0


Query: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASDSEE 60
           MEEQED         QLPQRIS AYVEQVNTD IMALAMQQQEHE  YT LETI SDSEE
Sbjct: 1   MEEQEDK--------QLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEE 60

Query: 61  DENSDSNSNNGLD-TNATSQRQELVSRWAFLEDDEE-TTDGNDDMEEDEDGDFEGFDLDE 120
           DE SDSNS+NGLD TN + Q QEL S W FL DDEE TTD N+ M+EDE+ D E FDLDE
Sbjct: 61  DERSDSNSSNGLDHTNDSFQSQELHSPWGFLADDEEETTDDNEYMDEDEEEDIEEFDLDE 120

Query: 121 LTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELA 180
           L+YEELIALGEFIGEEKRGLP+NEIPSCLHSSKFQTIEN+SGIDRCVICQVEY+D EELA
Sbjct: 121 LSYEELIALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELA 180

Query: 181 ALPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKN 216
           ALPCEHPYHSECI +WLQIKRVCPICGTEVSSP  SKN
Sbjct: 181 ALPCEHPYHSECISKWLQIKRVCPICGTEVSSPKPSKN 210

[Close]

BLAST of Cucsat.G4251 vs. ExPASy TrEMBL
Match: A0A0A0KFS4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G382920 PE=4 SV=1)

HSP 1 Score: 420 bits (1080), Expect = 2.71e-148
Identity = 216/217 (99.54%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASDSEE 60
           MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHE+AYTTLETIASDSEE
Sbjct: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHELAYTTLETIASDSEE 60

Query: 61  DENSDSNSNNGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGFDLDELT 120
           DENSDSNSNNGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGFDLDELT
Sbjct: 61  DENSDSNSNNGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGFDLDELT 120

Query: 121 YEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELAAL 180
           YEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELAAL
Sbjct: 121 YEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELAAL 180

Query: 181 PCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 217
           PCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE
Sbjct: 181 PCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 217

[Close]

BLAST of Cucsat.G4251 vs. ExPASy TrEMBL
Match: A0A1S3CT85 (E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucumis melo OX=3656 GN=LOC103504080 PE=4 SV=1)

HSP 1 Score: 367 bits (941), Expect = 6.16e-127
Identity = 196/230 (85.22%), Postives = 208/230 (90.43%), Query Frame = 0


Query: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASDSEE 60
           MEEQEDN +QQQQQ  LPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIAS+SEE
Sbjct: 1   MEEQEDNNKQQQQQ--LPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASESEE 60

Query: 61  DENSDSNSNNGLDTNATSQRQELVSRWAFLEDDEE---TTDG----------NDDMEEDE 120
           DE+SDSNSNNGL+TNA SQR+EL+ RWAF +++E      DG          N+DMEEDE
Sbjct: 61  DESSDSNSNNGLNTNAASQREELICRWAFPDENEYMEGNEDGDYEGSDMDELNEDMEEDE 120

Query: 121 DGDFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVIC 180
           DGDFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIEN+SGIDRCVIC
Sbjct: 121 DGDFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENKSGIDRCVIC 180

Query: 181 QVEYDDGEELAALPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 217
           QVEYDDGEELAALPC+HPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE
Sbjct: 181 QVEYDDGEELAALPCQHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 228

[Close]

BLAST of Cucsat.G4251 vs. ExPASy TrEMBL
Match: A0A5D3CF09 (E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold314G00370 PE=4 SV=1)

HSP 1 Score: 363 bits (933), Expect = 3.11e-122
Identity = 194/228 (85.09%), Postives = 206/228 (90.35%), Query Frame = 0


Query: 3   EQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASDSEEDE 62
           EQEDN +QQQQQ  LPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIAS+SEEDE
Sbjct: 241 EQEDNNKQQQQQ--LPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASESEEDE 300

Query: 63  NSDSNSNNGLDTNATSQRQELVSRWAFLEDDEE---TTDG----------NDDMEEDEDG 122
           +SDSNSNNGL+TNA SQR+EL+ RWAF +++E      DG          N+DMEEDEDG
Sbjct: 301 SSDSNSNNGLNTNAASQREELICRWAFPDENEYMEGNEDGDYEGSDMDELNEDMEEDEDG 360

Query: 123 DFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQV 182
           DFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIEN+SGIDRCVICQV
Sbjct: 361 DFEGFDLDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENKSGIDRCVICQV 420

Query: 183 EYDDGEELAALPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 217
           EYDDGEELAALPC+HPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE
Sbjct: 421 EYDDGEELAALPCQHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKNE 466

[Close]

BLAST of Cucsat.G4251 vs. ExPASy TrEMBL
Match: A0A6J1GTM7 (E3 ubiquitin ligase BIG BROTHER-related OS=Cucurbita moschata OX=3662 GN=LOC111457005 PE=4 SV=1)

HSP 1 Score: 319 bits (817), Expect = 2.61e-108
Identity = 174/218 (79.82%), Postives = 184/218 (84.40%), Query Frame = 0


Query: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASDSEE 60
           MEEQED         QLPQRIS AYVEQVNTD IMALAMQQQEHE  YT LETI SDSEE
Sbjct: 1   MEEQEDK--------QLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEE 60

Query: 61  DENSDSNSNNGLD-TNATSQRQELVSRWAFLEDDEE-TTDGNDDMEEDEDGDFEGFDLDE 120
           DE SDSNS+NGLD TN + Q QEL S W FL DDEE TTD N+ M+EDE+ D E FDLDE
Sbjct: 61  DERSDSNSSNGLDHTNDSFQSQELHSPWGFLADDEEETTDDNEYMDEDEEEDIEEFDLDE 120

Query: 121 LTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELA 180
           L+YEELIALGEFIGEEKRGLP+NEIPSCLHSSKFQTIEN+SGIDRCVICQVEY+D EELA
Sbjct: 121 LSYEELIALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELA 180

Query: 181 ALPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKN 216
           ALPCEHPYHSECI +WLQIKRVCPICGTEVSSP  SKN
Sbjct: 181 ALPCEHPYHSECISKWLQIKRVCPICGTEVSSPKPSKN 210

[Close]

BLAST of Cucsat.G4251 vs. ExPASy TrEMBL
Match: A0A6J1JR32 (E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC111487582 PE=4 SV=1)

HSP 1 Score: 317 bits (811), Expect = 2.14e-107
Identity = 173/218 (79.36%), Postives = 183/218 (83.94%), Query Frame = 0


Query: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASDSEE 60
           MEEQED         QLPQRIS AYVEQVNTD IMALAMQQQEHE  YT LETI SDSEE
Sbjct: 1   MEEQEDK--------QLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEE 60

Query: 61  DENSDSNSNNGLD-TNATSQRQELVSRWAFLEDDEE-TTDGNDDMEEDEDGDFEGFDLDE 120
           DE SDSNS+NGLD TN + Q QEL S W FL DDEE TTD N+ M+EDE+ D E FDLDE
Sbjct: 61  DERSDSNSSNGLDHTNDSLQSQELHSPWGFLADDEEETTDDNEYMDEDEEEDIEEFDLDE 120

Query: 121 LTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELA 180
           L+YEELIALGEFIGEEKRGLP+NEIPSCLHSSKFQTIEN+SGIDRCVICQVEY+D EELA
Sbjct: 121 LSYEELIALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELA 180

Query: 181 ALPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKN 216
           ALPCEHPYHSECI +WLQIKRVCPICGTEVSS   SKN
Sbjct: 181 ALPCEHPYHSECISKWLQIKRVCPICGTEVSSSKSSKN 210

[Close]

BLAST of Cucsat.G4251 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 1.8e-31
Identity = 83/215 (38.60%), Postives = 133/215 (61.86%), Query Frame = 0


Query: 6   DNKQQQQQQPQLPQRI-SLAYVEQVNTDLIMALAMQQQE-----HEMAYTTLETIASDSE 65
           DN++++ +QP  P ++  LA  EQ N+++ +A +          H+ + + +  I SD E
Sbjct: 2   DNQEEEPKQP--PNKLPDLALFEQANSEVALAASQANSHFAHAMHDSSPSMISMIESDEE 61

Query: 66  EDENSDSNSN--NGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDED--GDFEGFD 125
            ++  + N N     D+N     ++ ++   FLED E     N ++EE++D   + +  D
Sbjct: 62  SEDEEEINENYYEYFDSNGFGVDEDEINE--FLEDQE----SNSNLEEEDDFLEEEDEID 121

Query: 126 LDELTYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGE 185
            D+L+YEELIALG+FIG E RGL   EI +CL++S +    N++ IDRCV+CQ+E+++ E
Sbjct: 122 PDQLSYEELIALGDFIGVENRGLTPIEISTCLNASTYVFSHNKNEIDRCVVCQMEFEERE 181

Query: 186 ELAAL-PCEHPYHSECIGEWLQIKRVCPICGTEVS 210
            L  L PC+HPYHSECI +WL+ K++CPIC +E S
Sbjct: 182 SLVVLRPCDHPYHSECITKWLETKKICPICCSEPS 208

[Close]

BLAST of Cucsat.G4251 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 107.8 bits (268), Expect = 1.1e-23
Identity = 80/258 (31.01%), Postives = 121/258 (46.90%), Query Frame = 0


Query: 20  RISLAYVEQVNTDLIMALAMQQQEHEMAYTTLETIASD----------SEEDENSD---- 79
           R+    + Q++ DL +A  +Q+QE      T+ +  SD           +EDE  D    
Sbjct: 85  RVPFTNLSQIDADLALARTLQEQERAYMMLTMNSEISDYGSWETGSYVYDEDEFDDPENE 144

Query: 80  --SNSNNGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGF--------- 139
              +  +  +T+   Q   L       EDD+E  DGN D+EE    D E +         
Sbjct: 145 DEDDDEDEYETDDDPQEDGLDVNVHANEDDQE-DDGNSDIEEVAYTDDEAYARALQEAEE 204

Query: 140 -----------------------------------DLDELTYEELIALGEFIGEEKRGLP 199
                                              D DEL+YEEL+ALG+ +G E RGL 
Sbjct: 205 RDMAARLSALSGLANRVVEDLEDESHTSQDAWDEMDPDELSYEELLALGDIVGTESRGLS 264

Query: 200 INEIPSCLHSSKFQTIENRSGI-DRCVICQVEYDDGEELAALPCEHPYHSECIGEWLQIK 217
            + I S L S +++  +N++G  + CVIC+++Y+D E+L  LPC+H YHSECI  WL+I 
Sbjct: 265 ADTIAS-LPSKRYKEGDNQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKIN 324

[Close]

BLAST of Cucsat.G4251 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 8.6e-21
Identity = 53/143 (37.06%), Postives = 79/143 (55.24%), Query Frame = 0


Query: 75  NATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDF------EGFDLDELTYEELIALG 134
           N     +  V+R   + D +E ++  + +  + D D       +  D D +TYEEL+ LG
Sbjct: 100 NVADDPENTVARSVQIGDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELG 159

Query: 135 EFIGEEKRGLPINEIPSCLHSSKFQ---TIENRSGIDRCVICQVEYDDGEELAALPCEHP 194
           E +G E RGL   E+   L + K++       +   +RCVICQ++Y  GE    LPC+H 
Sbjct: 160 EAVGTESRGLS-QELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHV 219

Query: 195 YHSECIGEWLQIKRVCPICGTEV 209
           YHSECI +WL I +VCP+C +EV
Sbjct: 220 YHSECISKWLSINKVCPVCNSEV 241

[Close]

BLAST of Cucsat.G4251 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 8.6e-21
Identity = 53/143 (37.06%), Postives = 79/143 (55.24%), Query Frame = 0


Query: 75  NATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDF------EGFDLDELTYEELIALG 134
           N     +  V+R   + D +E ++  + +  + D D       +  D D +TYEEL+ LG
Sbjct: 100 NVADDPENTVARSVQIGDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELG 159

Query: 135 EFIGEEKRGLPINEIPSCLHSSKFQ---TIENRSGIDRCVICQVEYDDGEELAALPCEHP 194
           E +G E RGL   E+   L + K++       +   +RCVICQ++Y  GE    LPC+H 
Sbjct: 160 EAVGTESRGLS-QELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHV 219

Query: 195 YHSECIGEWLQIKRVCPICGTEV 209
           YHSECI +WL I +VCP+C +EV
Sbjct: 220 YHSECISKWLSINKVCPVCNSEV 241

[Close]

BLAST of Cucsat.G4251 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 1.0e-18
Identity = 42/98 (42.86%), Postives = 58/98 (59.18%), Query Frame = 0


Query: 115 DLDELTYEELIALGEFIGEEKRGLPINEIPSCLH-----SSKFQTIENRSGIDRCVICQV 174
           D+D ++YEEL+ALGE IG+   GL    I   +      SS   ++E    I+ C ICQ 
Sbjct: 602 DVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQE 661

Query: 175 EYDDGEELAALPCEHPYHSECIGEWLQIKRVCPICGTE 208
           EY +G+ L  L C H +H +CI +W+ IK +CPIC TE
Sbjct: 662 EYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

The following BLAST results are available for this feature:

BLAST of Cucsat.G4251 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LT17	1.5e-22	31.01	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]
Q8L649	1.2e-19	37.06	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9ZQF9	1.5e-17	42.86	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]
Q7XTV7	2.1e-16	41.30	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399...	[more]
Q9FMM4	4.7e-16	40.86	Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]

BLAST of Cucsat.G4251 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149804.1	5.60e-148	99.54	E3 ubiquitin ligase BIG BROTHER-related [Cucumis sativus] >KGN47704.1 hypothetic...	[more]
XP_008466738.1	1.27e-126	85.22	PREDICTED: E3 ubiquitin ligase BIG BROTHER-related-like [Cucumis melo]	[more]
XP_038886321.1	3.89e-126	87.50	E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida] >XP_038886322.1...	[more]
KAA0055464.1	6.43e-122	85.09	E3 ubiquitin ligase BIG BROTHER-related-like [Cucumis melo var. makuwa] >TYK0894...	[more]
XP_022954880.1	5.39e-108	79.82	E3 ubiquitin ligase BIG BROTHER-related [Cucurbita moschata]	[more]

BLAST of Cucsat.G4251 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KFS4	2.71e-148	99.54	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G382920 P...	[more]
A0A1S3CT85	6.16e-127	85.22	E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
A0A5D3CF09	3.11e-122	85.09	E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A6J1GTM7	2.61e-108	79.82	E3 ubiquitin ligase BIG BROTHER-related OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1JR32	2.14e-107	79.36	E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

BLAST of Cucsat.G4251 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47180.1	1.8e-31	38.60	RING/U-box superfamily protein	[more]
AT3G19910.1	1.1e-23	31.01	RING/U-box superfamily protein	[more]
AT3G63530.1	8.6e-21	37.06	RING/U-box superfamily protein	[more]
AT3G63530.2	8.6e-21	37.06	RING/U-box superfamily protein	[more]
AT2G15530.1	1.0e-18	42.86	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 130..170 e-value: 1.8E-5 score: 34.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 129..170 e-value: 1.7E-11 score: 44.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 130..170 score: 11.22052
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 106..179 e-value: 1.0E-17 score: 65.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..77
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..50
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 60..77
None	No IPR available	PANTHER	PTHR46400:SF8	E3 UBIQUITIN LIGASE BIG BROTHER-RELATED-LIKE	coord: 2..179
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 129..177
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 2..179

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G4251.T1	Cucsat.G4251.T1	mRNA
Cucsat.G4251.T2	Cucsat.G4251.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0019774	proteasome core complex, beta-subunit complex
molecular_function	GO:0004298	threonine-type endopeptidase activity
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity