Cucsat.G4053 (gene) Cucumber (B10) v3

Overview
NameCucsat.G4053
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionAB hydrolase-1 domain-containing protein
Locationctg105: 3226203 .. 3229608 (-)
RNA-Seq ExpressionCucsat.G4053
SyntenyCucsat.G4053
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGGAGGATAAAAGCAGGGTGGGACATTGAATTTAAACCATAATATTTTTGTGAAGTGAAAGTCCAAATTAAAACAAATTTAAATAGAACCCCAAGAACACATCCCACCGGATACACTGAAATAACTTCAAAATCCCCAAATTAAAGAGAGAATAATGCATAATTTGAATCTCCTTCTCCTCTTCTTCTTCTTCTTCTTCCTCTTTCTTTCTCCCACACTAACCAAATCCCTATCAGATCCGCCGCCTAGCACCGACGTAGGCGGCGGTGGAGGAAAGCACTTCGTCCTAGTTCACGGAGCCTGCCTGGGGGCCTGGTCGTGGTACAAGCTATCAACGCTGCTCCGATCGGCGGGGCATCGGGTGACAGCGTTAGACATGGCGGGGGCTGGAATTGATCCCCGGGAGGCGGAGAGGCTGAAATCGTTCAACGAATACGTTGAGCCGTTGAGAAATTTGATGGGGGAGGTGGGGGAGGAGGAGAAGGTGATTCTGGTAGGACACAGCCAAGGCGGGCTTTGCATATCGAAGGCAATGGAGGAATTTCCGGAGAAAATATCTGTTGCGGTTTTTGTGGTTGCCGCTATGCCTGGCCCAGCCCTAAACGCTTCATTTCTAATTGGACAGGTTTGAATCCGATCAATCTTCTTCTTCGTCTTACATTTCTTTTCAACAAATATCTTCTAAATAATAATTTTAAAAAAAGAATTCCTTTTAATTTTAATTTAAAATTAACTCTGTTTGTTGTTTTGTGTTAACTTTTGATTTTAAAATAATAAATTTATATAAGCAATCCATTCCCTTCAAAATATTCATTTCCCTCCCATAAGTTGTCGTTTGTGATGATTGTTGAAGTTGCTTGGTGAAAATTGGGAGTTGGAGGTGATTGGGGGATAAAGTTATGGGAAAATGTTTTAGAAGAGAGTTGGTGAGAGTGTTGAGTTGATTACTATAGTCAATAGTTGTTATGTTGGGTGAGGTCTATTTTAGTCTACCAACAAAAAGGTAAAGAGATGATGTGTGGAATATTATAAACACTATAACATATAGTAAATATTGTAACAAATAAATATTTGAAATAATTGTACCATAATTGAGCACAAGTATGGAGTTATAATATCTCTTTCCATCCACAAAGTTAGGGACCCATTGTCCAAATGCTCTTTGAGTTGTAAATAGTTTAATGTAGAAAAACGAGGGTTAAAATTAACTTTATTTTATTCATACCCTAGACACCAAATGAACTATTACAAGTTGCTCAAGCCACTCCAAGTTGGAGCTTTAAATACCCTTTAATAATTCTTCAAATTATAAAGTGAAATCAAAGAAAATTAACGATCGATTGAAACATCTATTAAGGAGATAGTTGGATTGACTAATTAAAAAAGATATTTTTAAAATCTATTATTATTTAAATTCTTTTGATAAAATCTATTTGAAATAAACTTTAAAGATTTTTTGAACGATGAAGTTCATCCTTTTCTTTTTTTCTTTCAATATTTAATTCACTTTCATTACAAACCTTTCTATAAAATCCAAAACCTCACATTAGAAGAAAAATAAAAGAAACCCATTGTATTTGTTTTAACATCAAAGTAAACTTCATCAATTGTTTTCACATAAATAAAATAAAAACATTAATGTATTGTTGTAGGTTAATTTTTATAAATATAACAAATCGACAAATAAAATATTTATGGTCCGTGTAACACAATGAAAAAGTCCATTAAGCTGGCTATTTTTTAAAAATGTTTTAGGTTTTCTCTCTTTTTTCTCCTTTTCCTTTTTGTGATTTTCCTTCTCCTTATCATTTTTTGCAATATTTTTTTTCAAACTATTATTTGGTTCAAAATCGTGTATCAAATATAAAAGATTTTAAAAAAATATATTTCAAATTGTTGTTTGGTTGTTTAGGTTGTGTGACAAATACAAAAAAAATGTACAAAATAACGAAATATAAACGATGATCGTGTACAAATCTAAACGATGTGTACAAAAAATAAATTACCTTTTATCAAATCTAAACAAAACGACCGTGTACCAAATAAATCATGAAAAAAATTATTGAGTTTTGACAATCCAAATTTAAACGATTAATGTAAATGATTGTATACTAAAAAATTATTAAAAAACCGTTTAGATTTAGATTTGGTCATTTATCATTTAGAATCTAGGAGAGAATAGCCTAATATAAATGATCATTTTCCAAATATTAAATGATCGTTTCCCAAATATTCTATGTGCTGGATGCTAATTAATCGGGGCATTTTGAAATTTTCTATTGTTTGCTTGGACAAATATTTTACTGGTTTGTTATACTTTTTTAAAAGATCTCATTCTTGTATGACATTTTATTTTGGATAATTATCTTGTACAACAACTTTTGGAACAAGTAATGAAGTATATAACAACATTTTAAAATATTTGTAAATATAATAAAATCTATCAAAGACTATATATCAATAATTGCAACATAGTTAGACAGTACAAATTTTAATATTTTAATATATCTGCAGTTACGGAAGTGGTTAGATTTTGGGCCAGACAGCCATTATACATATGGTAATGGGCCAAGAAGCCCACCAACAACTTTGACATTTGGGCCATTGTTCTTGGCCGCAAAAGTATTCAACAAAAGCCCCCTGGAGGTAAATCAATTTATTAAACAACTTTTCTAAATTCTACCATCTTCTTCTAAGTGCTTTAAAATCCTTCGATCCAGACTCTCGATTAACATCTCTCTGAAATATTACATGTTTCTGATACATTTATAAAACTCAACAAAAGTGGTTATAAAACGGAGTGATAGAACACGTTTTCGTTGTTGTTGTTCTTTTTTTGGTATGGTATAGGACTTGACATTAGGGAGGACATTGGTGAGACCAACCCATCTGTTTGGTGGAGAGCAATGGAACAAAGATTTAGTTTTGACAAAGGAGAGATATGGGTCTGTCAAAAGAGTTTTTGTTGTTTCGGATAATGATAAGGTCATCAAGAAAAGCTTCCAAAAATGGGTTATTCGTAGAAACCCACCTGATGGTGTTGTGGAAGTTAAAGGATCTGATCATATGGTCATGATGTCTAAGCCTTTGCATCTCTTCAACATACTTTCACACATTGCTCGTCAACATTCTTAATCCTCATTTGTATTGTTTTAGTATTCTGTTTCTTACTGCTGTGTAATAATCCTATATATTGTTGGGTTTTGGCAAAATATTAAAAGAGTGGTTGTAATATAAAAGAAGTTTGTATCTTTAGGGAGTGGTTGTCTTGTAGTTTTTCACTTGGTGTAATTTTTATGTACCACCACCATTGTTATCAACCCTTCAATCTTCGTTTCAAAATTTTCATTCACTACATATTGGCATTGCCATGTAGTTCTAATAATTTACCTTCAAACTCTTTTT

Coding sequence (CDS)

ATGCATAATTTGAATCTCCTTCTCCTCTTCTTCTTCTTCTTCTTCCTCTTTCTTTCTCCCACACTAACCAAATCCCTATCAGATCCGCCGCCTAGCACCGACGTAGGCGGCGGTGGAGGAAAGCACTTCGTCCTAGTTCACGGAGCCTGCCTGGGGGCCTGGTCGTGGTACAAGCTATCAACGCTGCTCCGATCGGCGGGGCATCGGGTGACAGCGTTAGACATGGCGGGGGCTGGAATTGATCCCCGGGAGGCGGAGAGGCTGAAATCGTTCAACGAATACGTTGAGCCGTTGAGAAATTTGATGGGGGAGGTGGGGGAGGAGGAGAAGGTGATTCTGGTAGGACACAGCCAAGGCGGGCTTTGCATATCGAAGGCAATGGAGGAATTTCCGGAGAAAATATCTGTTGCGGTTTTTGTGGTTGCCGCTATGCCTGGCCCAGCCCTAAACGCTTCATTTCTAATTGGACAGTTACGGAAGTGGTTAGATTTTGGGCCAGACAGCCATTATACATATGGTAATGGGCCAAGAAGCCCACCAACAACTTTGACATTTGGGCCATTGTTCTTGGCCGCAAAAGTATTCAACAAAAGCCCCCTGGAGGACTTGACATTAGGGAGGACATTGGTGAGACCAACCCATCTGTTTGGTGGAGAGCAATGGAACAAAGATTTAGTTTTGACAAAGGAGAGATATGGGTCTGTCAAAAGAGTTTTTGTTGTTTCGGATAATGATAAGGTCATCAAGAAAAGCTTCCAAAAATGGGTTATTCGTAGAAACCCACCTGATGGTGTTGTGGAAGTTAAAGGATCTGATCATATGGTCATGATGTCTAAGCCTTTGCATCTCTTCAACATACTTTCACACATTGCTCGTCAACATTCTTAA

Protein sequence

MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGGLCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPPTTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS
Homology
BLAST of Cucsat.G4053 vs. ExPASy Swiss-Prot
Match: Q6RYA0 (Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.0e-58
Identity = 122/262 (46.56%), Postives = 163/262 (62.21%), Query Frame = 0

Query: 40  GKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLR 99
           GKHFVLVHGAC G WSWYKL  LL +AGH+VTALD+A +G D R+ E L++  +Y  PL 
Sbjct: 4   GKHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPLM 63

Query: 100 NLMGEVGEEEKVILVGHSQGGLCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLR 159
            LM  +  +EKVILVGHS GG+ +  AME++P+KI  AVF+ A MP    N+SF++ Q  
Sbjct: 64  ELMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLEQYN 123

Query: 160 K------WLDFGPDSHYTYGNGPRSPPTTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPT 219
           +      WLD        YG+ P  P T++ FGP FLA K++     EDL L  +LVRP+
Sbjct: 124 ERTPAENWLD---TQFLPYGS-PEEPLTSMFFGPKFLAHKLYQLCSPEDLALASSLVRPS 183

Query: 220 HLFGGEQWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDH 279
            LF  E  +K    T ER+GSVKRV++V   DK I + FQ+W I        +E+KG+DH
Sbjct: 184 SLF-MEDLSKAKYFTDERFGSVKRVYIVCTEDKGIPEEFQRWQIDNIGVTEAIEIKGADH 243

Query: 280 MVMMSKPLHLFNILSHIARQHS 296
           M M+ +P  L   L  IA +++
Sbjct: 244 MAMLCEPQKLCASLLEIAHKYN 260

BLAST of Cucsat.G4053 vs. ExPASy Swiss-Prot
Match: Q9SE93 (Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 4.9e-56
Identity = 114/254 (44.88%), Postives = 157/254 (61.81%), Query Frame = 0

Query: 41  KHFVLVHGACLGAWSWYKLSTLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRN 100
           KHFVLVHG CLGAW WYKL  LL SAGH+VTA+D++ AGI+PR  + + +F +Y EPL  
Sbjct: 11  KHFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIHTFRDYSEPLME 70

Query: 101 LMGEVGEEEKVILVGHSQGGLCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRK 160
           +M  +  +EKV+L+GHS GG+ +  AME +PEKISVAVF+ A MP P  + ++   +  +
Sbjct: 71  VMASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDPNHSLTYPFEKYNE 130

Query: 161 W--LDFGPDSHY-TYGNGPRSPPTTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFG 220
               D   DS + TYGN P +P  ++  GP F+A K+F    +EDL L + L RP  LF 
Sbjct: 131 KCPADMMLDSQFSTYGN-PENPGMSMILGPQFMALKMFQNCSVEDLELAKMLTRPGSLF- 190

Query: 221 GEQWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMM 280
            +   K    + ERYGSVKR ++  + DK     FQKW +     D V E+K +DHM M+
Sbjct: 191 FQDLAKAKKFSTERYGSVKRAYIFCNEDKSFPVEFQKWFVESVGADKVKEIKEADHMGML 250

Query: 281 SKPLHLFNILSHIA 292
           S+P  +   L  I+
Sbjct: 251 SQPREVCKCLLDIS 262

BLAST of Cucsat.G4053 vs. ExPASy Swiss-Prot
Match: Q8S9K8 (Methylesterase 10 OS=Arabidopsis thaliana OX=3702 GN=MES10 PE=2 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.9e-55
Identity = 108/256 (42.19%), Postives = 157/256 (61.33%), Query Frame = 0

Query: 42  HFVLVHGACLGAWSWYKLSTLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNL 101
           HFV VHG+C GAW W+KL+  L+  GHRVTA+D+ G+G+D R+   ++  + Y+EPL + 
Sbjct: 21  HFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVSAYLEPLMSF 80

Query: 102 MGEVGEEEKVILVGHSQGGLCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKW 161
           M  + E EKV+LVGHS GG+  S AME FP K+SV +F+ A MP      + LI +    
Sbjct: 81  MESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMPHHDSPPAVLIQEYFTR 140

Query: 162 L--DFGPDSHYTYGNGPRSPPTTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGE 221
           L   F  D  +T+  G   PP+++ FG  FL  K ++   LEDL L   L++P+ L+  E
Sbjct: 141 LPEGFAMDCEFTFEEGLEHPPSSVLFGTSFLKEKAYSNCQLEDLELAMALMKPSWLYTKE 200

Query: 222 QWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSK 281
              +DL+ TKERYGS KRVF+V + D V+ +  QKW+I    P  V  ++ + HM M++K
Sbjct: 201 MGGEDLI-TKERYGSGKRVFIVCEGDNVVPEEIQKWMISNYEPHEVKRIEEAGHMAMLTK 260

Query: 282 PLHLFNILSHIARQHS 296
           P  L  +L  IA +++
Sbjct: 261 PHELSQLLQEIAAKYN 275

BLAST of Cucsat.G4053 vs. ExPASy Swiss-Prot
Match: A2WYS8 (Probable esterase PIR7A OS=Oryza sativa subsp. indica OX=39946 GN=PIR7A PE=3 SV=2)

HSP 1 Score: 210.7 bits (535), Expect = 2.3e-53
Identity = 111/259 (42.86%), Postives = 153/259 (59.07%), Query Frame = 0

Query: 39  GGKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPL 98
           GGKHFV VHG   GAW WY++   LR+AGHR TALDMA AG  P  A+ + S  EY  PL
Sbjct: 4   GGKHFVFVHGLGHGAWCWYRVVAALRAAGHRATALDMAAAGAHPARADEVGSLEEYSRPL 63

Query: 99  RNLMGEVGEEEKVILVGHSQGGLCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQL 158
            + +      E+++LVGHS GGL ++ AME FP+K++ AVF+ A MP    +    + + 
Sbjct: 64  LDAVAAAAPGERLVLVGHSLGGLSLALAMERFPDKVAAAVFLAACMPAAGKHMGITLEEF 123

Query: 159 RKWL--DFGPDSHYTYGNGPRSPPTTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRP-THL 218
            + +  DF  DS     N  + P T +  GP  LA K++N+SP EDLTL   LVRP T+ 
Sbjct: 124 MRRIKPDFFMDSKTIVLNTNQEPRTAVLLGPKLLAEKLYNRSPPEDLTLATMLVRPGTNY 183

Query: 219 FGGEQWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMV 278
                   + +LT+  YGSVKRVF+V+ +D    +  Q+W I  +P   V E+ G+DHM 
Sbjct: 184 IDDPIMKDETLLTEGNYGSVKRVFLVAMDDASSDEEMQRWTIDLSPGVEVEELAGADHMA 243

Query: 279 MMSKPLHLFNILSHIARQH 295
           M SKP  L ++L  IA ++
Sbjct: 244 MCSKPRELCDLLLRIAAKY 262

BLAST of Cucsat.G4053 vs. ExPASy Swiss-Prot
Match: Q0JG98 (Probable esterase PIR7A OS=Oryza sativa subsp. japonica OX=39947 GN=PIR7A PE=3 SV=2)

HSP 1 Score: 210.7 bits (535), Expect = 2.3e-53
Identity = 111/259 (42.86%), Postives = 153/259 (59.07%), Query Frame = 0

Query: 39  GGKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPL 98
           GGKHFV VHG   GAW WY++   LR+AGHR TALDMA AG  P  A+ + S  EY  PL
Sbjct: 4   GGKHFVFVHGLGHGAWCWYRVVAALRAAGHRATALDMAAAGAHPARADEVGSLEEYSRPL 63

Query: 99  RNLMGEVGEEEKVILVGHSQGGLCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQL 158
            + +      E+++LVGHS GGL ++ AME FP+K++ AVF+ A MP    +    + + 
Sbjct: 64  LDAVAAAAPGERLVLVGHSLGGLSLALAMERFPDKVAAAVFLAACMPAAGKHMGITLEEF 123

Query: 159 RKWL--DFGPDSHYTYGNGPRSPPTTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRP-THL 218
            + +  DF  DS     N  + P T +  GP  LA K++N+SP EDLTL   LVRP T+ 
Sbjct: 124 MRRIKPDFFMDSKTIVLNTNQEPRTAVLLGPKLLAEKLYNRSPPEDLTLATMLVRPGTNY 183

Query: 219 FGGEQWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMV 278
                   + +LT+  YGSVKRVF+V+ +D    +  Q+W I  +P   V E+ G+DHM 
Sbjct: 184 IDDPIMKDETLLTEGNYGSVKRVFLVAMDDASSDEEMQRWTIDLSPGVEVEELAGADHMA 243

Query: 279 MMSKPLHLFNILSHIARQH 295
           M SKP  L ++L  IA ++
Sbjct: 244 MCSKPRELCDLLLRIAAKY 262

BLAST of Cucsat.G4053 vs. NCBI nr
Match: XP_004153671.1 (salicylic acid-binding protein 2 [Cucumis sativus] >KGN61744.1 hypothetical protein Csa_006815 [Cucumis sativus])

HSP 1 Score: 598 bits (1542), Expect = 8.49e-216
Identity = 295/295 (100.00%), Postives = 295/295 (100.00%), Query Frame = 0

Query: 1   MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLS 60
           MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLS
Sbjct: 1   MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLS 60

Query: 61  TLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGG 120
           TLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGG
Sbjct: 61  TLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGG 120

Query: 121 LCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPP 180
           LCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPP
Sbjct: 121 LCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPP 180

Query: 181 TTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFV 240
           TTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFV
Sbjct: 181 TTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFV 240

Query: 241 VSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295
           VSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS
Sbjct: 241 VSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295

BLAST of Cucsat.G4053 vs. NCBI nr
Match: XP_008459227.1 (PREDICTED: salicylic acid-binding protein 2-like [Cucumis melo])

HSP 1 Score: 516 bits (1330), Expect = 1.35e-183
Identity = 263/295 (89.15%), Postives = 272/295 (92.20%), Query Frame = 0

Query: 1   MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLS 60
           MH LNLL     FFFLFLSPTLTKSLSDPPPS DV  GG KHFVLVHGACLGAWSWYKLS
Sbjct: 1   MHYLNLL-----FFFLFLSPTLTKSLSDPPPSPDVSSGG-KHFVLVHGACLGAWSWYKLS 60

Query: 61  TLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGG 120
           TLLRSAGHRVTALDMAGAGIDPREAE L +FNEYVEPLR+ M EVGE+EKVILVGHSQGG
Sbjct: 61  TLLRSAGHRVTALDMAGAGIDPREAESL-TFNEYVEPLRSFMAEVGEDEKVILVGHSQGG 120

Query: 121 LCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPP 180
           LCISKAMEE+PEKI VAVFVVAAMPGPALNASFLIG+LRK LDFGPDS YTYGNGP SPP
Sbjct: 121 LCISKAMEEYPEKICVAVFVVAAMPGPALNASFLIGELRKRLDFGPDSRYTYGNGPTSPP 180

Query: 181 TTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFV 240
           TTLTFGPLFLAA VFNKSP EDLTLGR LVRPTHLFGGEQWNKDLVLTKERYG+VKRVFV
Sbjct: 181 TTLTFGPLFLAASVFNKSPPEDLTLGRILVRPTHLFGGEQWNKDLVLTKERYGAVKRVFV 240

Query: 241 VSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295
           VSD DKVIKK+FQKWVIRRNPP+GVVEVKGSDHMVMMSKPL LFNILSHIARQ+S
Sbjct: 241 VSDKDKVIKKNFQKWVIRRNPPNGVVEVKGSDHMVMMSKPLDLFNILSHIARQYS 288

BLAST of Cucsat.G4053 vs. NCBI nr
Match: XP_038900290.1 (LOW QUALITY PROTEIN: salicylic acid-binding protein 2-like [Benincasa hispida])

HSP 1 Score: 467 bits (1201), Expect = 5.43e-164
Identity = 234/295 (79.32%), Postives = 256/295 (86.78%), Query Frame = 0

Query: 1   MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLS 60
           MH LN+LL     FFLFLS TL K+ SDPP S      GGKHFVLVHGACLGAWSWYKL 
Sbjct: 1   MHYLNILL-----FFLFLSSTLAKTKSDPPSSP-----GGKHFVLVHGACLGAWSWYKLY 60

Query: 61  TLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGG 120
           TLLRSAGHRVT LDMAGAGIDPREAE LKSF+EYV+PL + M E  EEEKVILVGHSQGG
Sbjct: 61  TLLRSAGHRVTVLDMAGAGIDPREAESLKSFSEYVQPLTDFMAEXAEEEKVILVGHSQGG 120

Query: 121 LCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPP 180
           LCISKAME+FP+KISVA+FV AAMPGP+LNASFL+ QLRK +DFGPDS YT+GNGP+SPP
Sbjct: 121 LCISKAMEDFPDKISVAIFVAAAMPGPSLNASFLLQQLRKRIDFGPDSRYTFGNGPKSPP 180

Query: 181 TTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFV 240
           TTLTFGPLFLA+K+FNKSP EDLTLGRTL+RPTHLF G+QWNKDLVLTK+RYG+VKRVFV
Sbjct: 181 TTLTFGPLFLASKLFNKSPKEDLTLGRTLMRPTHLFEGQQWNKDLVLTKQRYGAVKRVFV 240

Query: 241 VSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295
           V D DKVI+KSFQKWVIRRNPP  VVEV GSDHMVMMSKPL LF+ LSHIA  +S
Sbjct: 241 VLDKDKVIQKSFQKWVIRRNPPTAVVEVTGSDHMVMMSKPLDLFHKLSHIAHHYS 285

BLAST of Cucsat.G4053 vs. NCBI nr
Match: KAG7035115.1 (Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 446 bits (1147), Expect = 9.74e-156
Identity = 218/282 (77.30%), Postives = 249/282 (88.30%), Query Frame = 0

Query: 14  FFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTAL 73
           FFLFLS T+T + S+PPPS   GGG GKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTAL
Sbjct: 9   FFLFLSSTVTLTRSNPPPS---GGGDGKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTAL 68

Query: 74  DMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGGLCISKAMEEFPEK 133
           DMAGAGIDPREAE L+SF+EYV+PL + M ++  EEKVILVGHSQGGLCISKAME+FP+K
Sbjct: 69  DMAGAGIDPREAEDLRSFSEYVQPLTDFMAKLPAEEKVILVGHSQGGLCISKAMEDFPDK 128

Query: 134 ISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPPTTLTFGPLFLAAK 193
           ISVAVFV AAMPGP+LNASFL+ Q RK +DFGPD+ YT+GNGP+SPPTTLTFGPLF A K
Sbjct: 129 ISVAVFVAAAMPGPSLNASFLLEQFRKRIDFGPDTRYTFGNGPKSPPTTLTFGPLFSATK 188

Query: 194 VFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQ 253
           +F++SP +DLTLG TL+R THLF G QW+KDL LT +RYGSVKRVFVVS+ DKVIKKSFQ
Sbjct: 189 LFDRSPKQDLTLGNTLMRQTHLFVGRQWSKDLPLTTQRYGSVKRVFVVSEKDKVIKKSFQ 248

Query: 254 KWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295
           +WVI+RNPP  VVEV+GSDHMVM+SKP  LFN LSHIAR++S
Sbjct: 249 QWVIKRNPPTDVVEVRGSDHMVMISKPFDLFNKLSHIARRYS 287

BLAST of Cucsat.G4053 vs. NCBI nr
Match: XP_022947435.1 (salicylic acid-binding protein 2-like [Cucurbita moschata])

HSP 1 Score: 446 bits (1147), Expect = 9.74e-156
Identity = 218/282 (77.30%), Postives = 249/282 (88.30%), Query Frame = 0

Query: 14  FFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTAL 73
           FFLFLS T+T + S+PPPS   GGG GKHFVLVHGACLGAWSWYKLSTLLRS GHRVTAL
Sbjct: 9   FFLFLSSTVTLTRSNPPPS---GGGDGKHFVLVHGACLGAWSWYKLSTLLRSVGHRVTAL 68

Query: 74  DMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGGLCISKAMEEFPEK 133
           DMAGAGIDPREAE L+SF+EYV+PL + M ++  EEKVILVGHSQGGLCISKAME+FP+K
Sbjct: 69  DMAGAGIDPREAEDLRSFSEYVQPLTDFMAKLPAEEKVILVGHSQGGLCISKAMEDFPDK 128

Query: 134 ISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPPTTLTFGPLFLAAK 193
           ISVAVFVVAAMPGP+LNASFL+ Q RK +DFGPD+ YT+GNGP+SPPTTLTFGPLF A K
Sbjct: 129 ISVAVFVVAAMPGPSLNASFLLEQFRKRIDFGPDTRYTFGNGPKSPPTTLTFGPLFSATK 188

Query: 194 VFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQ 253
           +F++SP +DLTLG TL+R THLF G QW+KDL LT +RYGSVKRVFVVS+ DKVIKKSFQ
Sbjct: 189 LFDRSPKQDLTLGNTLMRQTHLFVGRQWSKDLPLTTQRYGSVKRVFVVSEKDKVIKKSFQ 248

Query: 254 KWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295
           +WVI+RNPP  VVEV+GSDHMVM+SKP  LFN LSHIAR++S
Sbjct: 249 QWVIKRNPPTDVVEVRGSDHMVMISKPFDLFNKLSHIARRYS 287

BLAST of Cucsat.G4053 vs. ExPASy TrEMBL
Match: A0A0A0LL04 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G237140 PE=4 SV=1)

HSP 1 Score: 598 bits (1542), Expect = 4.11e-216
Identity = 295/295 (100.00%), Postives = 295/295 (100.00%), Query Frame = 0

Query: 1   MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLS 60
           MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLS
Sbjct: 1   MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLS 60

Query: 61  TLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGG 120
           TLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGG
Sbjct: 61  TLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGG 120

Query: 121 LCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPP 180
           LCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPP
Sbjct: 121 LCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPP 180

Query: 181 TTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFV 240
           TTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFV
Sbjct: 181 TTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFV 240

Query: 241 VSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295
           VSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS
Sbjct: 241 VSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295

BLAST of Cucsat.G4053 vs. ExPASy TrEMBL
Match: A0A1S3C9T3 (salicylic acid-binding protein 2-like OS=Cucumis melo OX=3656 GN=LOC103498416 PE=4 SV=1)

HSP 1 Score: 516 bits (1330), Expect = 6.54e-184
Identity = 263/295 (89.15%), Postives = 272/295 (92.20%), Query Frame = 0

Query: 1   MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLS 60
           MH LNLL     FFFLFLSPTLTKSLSDPPPS DV  GG KHFVLVHGACLGAWSWYKLS
Sbjct: 1   MHYLNLL-----FFFLFLSPTLTKSLSDPPPSPDVSSGG-KHFVLVHGACLGAWSWYKLS 60

Query: 61  TLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGG 120
           TLLRSAGHRVTALDMAGAGIDPREAE L +FNEYVEPLR+ M EVGE+EKVILVGHSQGG
Sbjct: 61  TLLRSAGHRVTALDMAGAGIDPREAESL-TFNEYVEPLRSFMAEVGEDEKVILVGHSQGG 120

Query: 121 LCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPP 180
           LCISKAMEE+PEKI VAVFVVAAMPGPALNASFLIG+LRK LDFGPDS YTYGNGP SPP
Sbjct: 121 LCISKAMEEYPEKICVAVFVVAAMPGPALNASFLIGELRKRLDFGPDSRYTYGNGPTSPP 180

Query: 181 TTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFV 240
           TTLTFGPLFLAA VFNKSP EDLTLGR LVRPTHLFGGEQWNKDLVLTKERYG+VKRVFV
Sbjct: 181 TTLTFGPLFLAASVFNKSPPEDLTLGRILVRPTHLFGGEQWNKDLVLTKERYGAVKRVFV 240

Query: 241 VSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295
           VSD DKVIKK+FQKWVIRRNPP+GVVEVKGSDHMVMMSKPL LFNILSHIARQ+S
Sbjct: 241 VSDKDKVIKKNFQKWVIRRNPPNGVVEVKGSDHMVMMSKPLDLFNILSHIARQYS 288

BLAST of Cucsat.G4053 vs. ExPASy TrEMBL
Match: A0A6J1L366 (salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111499981 PE=4 SV=1)

HSP 1 Score: 446 bits (1147), Expect = 4.71e-156
Identity = 219/282 (77.66%), Postives = 248/282 (87.94%), Query Frame = 0

Query: 14  FFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTAL 73
           FFLFLS T+T + S+PPPS   GGG GKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTAL
Sbjct: 9   FFLFLSSTVTLTRSNPPPS---GGGDGKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTAL 68

Query: 74  DMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGGLCISKAMEEFPEK 133
           DMAGAGIDPREAE LKSF+EYV+PL + M ++  EEKVILVGHSQGGLCISKAME+FP+K
Sbjct: 69  DMAGAGIDPREAEDLKSFSEYVQPLTDFMAKLPAEEKVILVGHSQGGLCISKAMEDFPDK 128

Query: 134 ISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPPTTLTFGPLFLAAK 193
           ISVAVFV AAMPGP+LNASFL+ Q  K +DFGPDS YT+GNGP+SPPTTL FGPLF A K
Sbjct: 129 ISVAVFVAAAMPGPSLNASFLLEQFSKRIDFGPDSRYTFGNGPKSPPTTLKFGPLFSATK 188

Query: 194 VFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQ 253
           +F++SP +DLTLG TL+R THLF G QW+KDL LT +RYGSVKRVFVVS+ DKVIKKSFQ
Sbjct: 189 LFDRSPKQDLTLGNTLMRQTHLFVGRQWSKDLPLTTQRYGSVKRVFVVSEKDKVIKKSFQ 248

Query: 254 KWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295
           +WVI+RNPP+ VVEV+GSDHMVMMSKP  LFN LSHIAR++S
Sbjct: 249 QWVIKRNPPNDVVEVRGSDHMVMMSKPFDLFNKLSHIARRYS 287

BLAST of Cucsat.G4053 vs. ExPASy TrEMBL
Match: A0A6J1G6W0 (salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111451294 PE=4 SV=1)

HSP 1 Score: 446 bits (1147), Expect = 4.71e-156
Identity = 218/282 (77.30%), Postives = 249/282 (88.30%), Query Frame = 0

Query: 14  FFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLSTLLRSAGHRVTAL 73
           FFLFLS T+T + S+PPPS   GGG GKHFVLVHGACLGAWSWYKLSTLLRS GHRVTAL
Sbjct: 9   FFLFLSSTVTLTRSNPPPS---GGGDGKHFVLVHGACLGAWSWYKLSTLLRSVGHRVTAL 68

Query: 74  DMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGGLCISKAMEEFPEK 133
           DMAGAGIDPREAE L+SF+EYV+PL + M ++  EEKVILVGHSQGGLCISKAME+FP+K
Sbjct: 69  DMAGAGIDPREAEDLRSFSEYVQPLTDFMAKLPAEEKVILVGHSQGGLCISKAMEDFPDK 128

Query: 134 ISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPPTTLTFGPLFLAAK 193
           ISVAVFVVAAMPGP+LNASFL+ Q RK +DFGPD+ YT+GNGP+SPPTTLTFGPLF A K
Sbjct: 129 ISVAVFVVAAMPGPSLNASFLLEQFRKRIDFGPDTRYTFGNGPKSPPTTLTFGPLFSATK 188

Query: 194 VFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQ 253
           +F++SP +DLTLG TL+R THLF G QW+KDL LT +RYGSVKRVFVVS+ DKVIKKSFQ
Sbjct: 189 LFDRSPKQDLTLGNTLMRQTHLFVGRQWSKDLPLTTQRYGSVKRVFVVSEKDKVIKKSFQ 248

Query: 254 KWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295
           +WVI+RNPP  VVEV+GSDHMVM+SKP  LFN LSHIAR++S
Sbjct: 249 QWVIKRNPPTDVVEVRGSDHMVMISKPFDLFNKLSHIARRYS 287

BLAST of Cucsat.G4053 vs. ExPASy TrEMBL
Match: A0A6J1D5N6 (salicylic acid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC111017157 PE=4 SV=1)

HSP 1 Score: 417 bits (1073), Expect = 8.35e-145
Identity = 209/295 (70.85%), Postives = 239/295 (81.02%), Query Frame = 0

Query: 1   MHNLNLLLLFFFFFFLFLSPTLTKSLSDPPPSTDVGGGGGKHFVLVHGACLGAWSWYKLS 60
           M NLNLLLL   F FLFL  ++ K+ SDP P          HFVLVHGAC GAWSWYK++
Sbjct: 1   MPNLNLLLL--LFLFLFLCSSVAKTRSDPAPRRH-------HFVLVHGACHGAWSWYKVA 60

Query: 61  TLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNLMGEVGEEEKVILVGHSQGG 120
            LLRSAGHRVTA+DMAG+GID REAERL+SF+EYV PLR+ M  V  EEKV+LVGHSQGG
Sbjct: 61  ALLRSAGHRVTAVDMAGSGIDRREAERLRSFSEYVGPLRDAMAAVAAEEKVVLVGHSQGG 120

Query: 121 LCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKWLDFGPDSHYTYGNGPRSPP 180
           LCISKAME FP+KISVAVFV AAMPGP+LN SFL+ Q+ + +DFGPDS YTYGNGPR+PP
Sbjct: 121 LCISKAMESFPDKISVAVFVAAAMPGPSLNVSFLLQQMLRRVDFGPDSRYTYGNGPRNPP 180

Query: 181 TTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQWNKDLVLTKERYGSVKRVFV 240
           TTLTFGPLF A+K++N SP EDLTL  TL+RPTHLFG +QW+KDL LT ERYGSVKRVFV
Sbjct: 181 TTLTFGPLFSASKLYNNSPKEDLTLANTLMRPTHLFGTQQWSKDLNLTVERYGSVKRVFV 240

Query: 241 VSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKPLHLFNILSHIARQHS 295
           VS ND++I K FQ WVI+RNPP  VVEV+GSDHMVMMSKP HLF  L  IAR ++
Sbjct: 241 VSGNDRIINKKFQWWVIKRNPPTAVVEVRGSDHMVMMSKPFHLFKKLHRIARDYA 286

BLAST of Cucsat.G4053 vs. TAIR 10
Match: AT3G50440.1 (methyl esterase 10 )

HSP 1 Score: 217.6 bits (553), Expect = 1.3e-56
Identity = 108/256 (42.19%), Postives = 157/256 (61.33%), Query Frame = 0

Query: 42  HFVLVHGACLGAWSWYKLSTLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRNL 101
           HFV VHG+C GAW W+KL+  L+  GHRVTA+D+ G+G+D R+   ++  + Y+EPL + 
Sbjct: 34  HFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVSAYLEPLMSF 93

Query: 102 MGEVGEEEKVILVGHSQGGLCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRKW 161
           M  + E EKV+LVGHS GG+  S AME FP K+SV +F+ A MP      + LI +    
Sbjct: 94  MESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMPHHDSPPAVLIQEYFTR 153

Query: 162 L--DFGPDSHYTYGNGPRSPPTTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGE 221
           L   F  D  +T+  G   PP+++ FG  FL  K ++   LEDL L   L++P+ L+  E
Sbjct: 154 LPEGFAMDCEFTFEEGLEHPPSSVLFGTSFLKEKAYSNCQLEDLELAMALMKPSWLYTKE 213

Query: 222 QWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSK 281
              +DL+ TKERYGS KRVF+V + D V+ +  QKW+I    P  V  ++ + HM M++K
Sbjct: 214 MGGEDLI-TKERYGSGKRVFIVCEGDNVVPEEIQKWMISNYEPHEVKRIEEAGHMAMLTK 273

Query: 282 PLHLFNILSHIARQHS 296
           P  L  +L  IA +++
Sbjct: 274 PHELSQLLQEIAAKYN 288

BLAST of Cucsat.G4053 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 199.1 bits (505), Expect = 4.8e-51
Identity = 113/266 (42.48%), Postives = 158/266 (59.40%), Query Frame = 0

Query: 41  KHFVLVHGACLGAWSWYKLSTLLRSAGHRVTALDMAGAGID-PREAERLKSFNEYVEPLR 100
           +H VLVHGAC GAW WYK+   L ++GHRVTA+D+A +GID  R    + +  +Y EPL 
Sbjct: 8   QHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSEPLM 67

Query: 101 NLMGEVGEEEKVILVGHSQGGLCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLR 160
            LM  + ++EKV+LVGHS GGL ++ AM+ FP KISV+VFV A MP    + SF+  +LR
Sbjct: 68  QLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWDKLR 127

Query: 161 K------WLDFGPDSHYTYGNGPRSPPTTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPT 220
           K      WL    D+ +T    P  P     FGP F+A  ++  SP++DL L + LVR  
Sbjct: 128 KETSREEWL----DTVFT-SEKPDFPSEFWIFGPEFMAKNLYQLSPVQDLELAKMLVRAN 187

Query: 221 HLFGGEQWNKDLV----LTKERYGSVKRVFVVSDNDKVIKKSFQKWVIRRNPPDGVVEVK 280
            L       KD+      ++E YGSV R+F+V   D V  + +Q+ +I   PP  V+E+K
Sbjct: 188 PLI-----KKDMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMISNFPPKEVMEIK 247

Query: 281 GSDHMVMMSKPLHLFNILSHIARQHS 296
            +DHM M SKP  L  +L  IA +++
Sbjct: 248 DADHMPMFSKPQQLCALLLEIANKYA 263

BLAST of Cucsat.G4053 vs. TAIR 10
Match: AT2G23590.1 (methyl esterase 8 )

HSP 1 Score: 192.6 bits (488), Expect = 4.5e-49
Identity = 104/255 (40.78%), Postives = 155/255 (60.78%), Query Frame = 0

Query: 41  KHFVLVHGACLGAWSWYKLSTLLRSAGHRVTALDMAGAGIDPREAERLKSFNEYVEPLRN 100
           +HFVLVHG+CLGAW WYK+  LL ++GHRVTALD+A  GID R    + +  +Y EPL  
Sbjct: 26  QHFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQYSEPLIQ 85

Query: 101 LMGEVGEEEKVILVGHSQGGLCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLRK 160
           LM  +  +EKV+LVGHS GGL ++ AM++FP+KISV+VFV + MP    + SF++ +   
Sbjct: 86  LMTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMPDTKNSPSFVLEKFAS 145

Query: 161 WLDFGPDSHYTYGNGPRSPPTTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPTHLFGGEQ 220
            +   P+       G    P  + F   F   ++   SP+EDL L   L RP  LF  + 
Sbjct: 146 TMT--PEDWM----GSELEP-YVVFSAEFTKHRILQLSPIEDLELRLLLKRPGSLFLND- 205

Query: 221 WNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDHMVMMSKP 280
            ++    +++ YGSV R ++VS +D  I + +Q+W+I   PP+ V+E++G+DH+ +  KP
Sbjct: 206 LSRMKNFSEKGYGSVPRAYIVSKDDHTISEEYQRWMIDNYPPNLVIEMEGTDHLPLFCKP 265

Query: 281 LHLFNILSHIARQHS 296
             L + L  IA + S
Sbjct: 266 QLLSDHLLAIADKFS 272

BLAST of Cucsat.G4053 vs. TAIR 10
Match: AT2G23620.1 (methyl esterase 1 )

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-48
Identity = 110/260 (42.31%), Postives = 156/260 (60.00%), Query Frame = 0

Query: 41  KHFVLVHGACLGAWSWYKLSTLLRSAGHRVTALDMAGAGID-PREAERLKSFNEYVEPLR 100
           +HFVLVHG+C GAW WYK+  LL + GHRVTA+D+A +GID  R    + +  +Y EPL 
Sbjct: 8   QHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPLT 67

Query: 101 NLMGEVGEEEKVILVGHSQGGLCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQL- 160
            L+  +  +EKV+LVGHS GGL ++ AME+FPEKISVAVF+ A MP    + SF++ +  
Sbjct: 68  KLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKFG 127

Query: 161 -----RKWL--DFGPDSHYTYGNGPRSPPTTLTFGPLFLAAKVFNKSPLEDLTLGRTLVR 220
                  W+  +F P     YG+   +   ++ F P F+   ++  SP+EDL LG  L+R
Sbjct: 128 SNMPQEAWMGTEFEP-----YGSD--NSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMR 187

Query: 221 PTHLFGGEQWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQKWVIRRNPPDGVVEVKGS 280
           P  LF  +  +K    + E YGSV RVF+V   DK I +  Q+W+I   P + V+E++ +
Sbjct: 188 PGSLFIND-LSKMKNFSDEGYGSVPRVFIVCKEDKAIPEERQRWMIDNFPVNLVMEMEET 247

Query: 281 DHMVMMSKPLHLFNILSHIA 292
           DHM M  KP  L +    IA
Sbjct: 248 DHMPMFCKPQQLSDYFLKIA 259

BLAST of Cucsat.G4053 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 188.7 bits (478), Expect = 6.5e-48
Identity = 107/258 (41.47%), Postives = 154/258 (59.69%), Query Frame = 0

Query: 41  KHFVLVHGACLGAWSWYKLSTLLRSAGHRVTALDMAGAGID-PREAERLKSFNEYVEPLR 100
           +HFVLVHGAC GAW WYK+  LL + GHRVTALD+A +GID  R    + +  +Y EPL 
Sbjct: 8   QHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPLM 67

Query: 101 NLMGEVGEEEKVILVGHSQGGLCISKAMEEFPEKISVAVFVVAAMPGPALNASFLIGQLR 160
            LM  +  +EKV+LVGHS GGL ++ AM++FP+KISV+VFV A MP    + SF+  +  
Sbjct: 68  QLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKFA 127

Query: 161 K------WLDFGPDSHYTYGNGPRSPPTTLTFGPLFLAAKVFNKSPLEDLTLGRTLVRPT 220
                  W+        TYG+   +   ++ F   F+  +++  SP+EDL LG  L RP+
Sbjct: 128 SSMTPEGWMG---SELETYGSD--NSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPS 187

Query: 221 HLFGGEQWNKDLVLTKERYGSVKRVFVVSDNDKVIKKSFQKWVIRRNPPDGVVEVKGSDH 280
            LF  E  +K    +++ YGSV R ++V   D +I +  Q+W+I   P + V+E++ +DH
Sbjct: 188 SLFINE-LSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDH 247

Query: 281 MVMMSKPLHLFNILSHIA 292
           M M  KP  L + L  IA
Sbjct: 248 MPMFCKPQLLSDHLLAIA 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6RYA01.0e-5846.56Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1[more]
Q9SE934.9e-5644.88Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=... [more]
Q8S9K81.9e-5542.19Methylesterase 10 OS=Arabidopsis thaliana OX=3702 GN=MES10 PE=2 SV=1[more]
A2WYS82.3e-5342.86Probable esterase PIR7A OS=Oryza sativa subsp. indica OX=39946 GN=PIR7A PE=3 SV=... [more]
Q0JG982.3e-5342.86Probable esterase PIR7A OS=Oryza sativa subsp. japonica OX=39947 GN=PIR7A PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_004153671.18.49e-216100.00salicylic acid-binding protein 2 [Cucumis sativus] >KGN61744.1 hypothetical prot... [more]
XP_008459227.11.35e-18389.15PREDICTED: salicylic acid-binding protein 2-like [Cucumis melo][more]
XP_038900290.15.43e-16479.32LOW QUALITY PROTEIN: salicylic acid-binding protein 2-like [Benincasa hispida][more]
KAG7035115.19.74e-15677.30Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. argyros... [more]
XP_022947435.19.74e-15677.30salicylic acid-binding protein 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0LL044.11e-216100.00AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G237... [more]
A0A1S3C9T36.54e-18489.15salicylic acid-binding protein 2-like OS=Cucumis melo OX=3656 GN=LOC103498416 PE... [more]
A0A6J1L3664.71e-15677.66salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11149998... [more]
A0A6J1G6W04.71e-15677.30salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111451... [more]
A0A6J1D5N68.35e-14570.85salicylic acid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC11101... [more]
Match NameE-valueIdentityDescription
AT3G50440.11.3e-5642.19methyl esterase 10 [more]
AT2G23610.14.8e-5142.48methyl esterase 3 [more]
AT2G23590.14.5e-4940.78methyl esterase 8 [more]
AT2G23620.11.3e-4842.31methyl esterase 1 [more]
AT2G23600.16.5e-4841.47acetone-cyanohydrin lyase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 40..210
e-value: 1.3E-40
score: 141.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 36..157
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 43..177
e-value: 6.4E-14
score: 53.1
NoneNo IPR availablePANTHERPTHR10992:SF1002ESTERASE PIR7A-RELATEDcoord: 37..203
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 37..203

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G4053.T1Cucsat.G4053.T1mRNA
Cucsat.G4053.T2Cucsat.G4053.T2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity