Cucsat.G3997 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G3997
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	WAT1-related protein
Location	ctg105: 1824503 .. 1825392 (-)
RNA-Seq Expression	Cucsat.G3997
Synteny	Cucsat.G3997

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAATCCAAAAGGAATAGCAAAAGTAATGGGGACATTGGTTTCATTGGGTGGTGTGTTGATAATGACATTCTATAAAGGACCTATAATTAGAAATGTATGCCAACCTCTCATCCATATTCAACACAAAGCTACTTATCATCTCCATGAGGATTGGCTTAAGGGTTCACTTCTTACCGTTTCAAGCTGCCTTTCTTGGGCCATTTCCTACATTTTGCAGGTCCTTAATCATTTATAGTAATCCTTACTTTTTTTCACCTAAAGTACTTACTATGTTAGATGAACCTAAAATCTCACTACACAAAACTTAAAACTTATAACGTGCTTAAACCTCAAGGATAACCAAAAGTACCATTTCGCATAAATTCTTTAATTTCTTAATCCCTCATATCTTACAAATCATTTCAAAAGATACACGTAACGATTAAGTTTAAACTTTTCGATATTGAAAACTGTAGGCTTTTACGTTAAAGCGATACCCAGCACCGTTGTCGTTAACGACGTGGATGAACCTGTTTGGAGCAGTACAAACAGCTGTGTACACAGTGTCGACACAACACAAGGCAGGAGTTTGGAATATTGGATTCAACATTGACCTCTGGGCCATTATTTATGCAGTAAGGAAATTTATTGAGCTGAAATCCCTTACCGCGTTTGGTAACCATTTTATAAGATAAAAGAAAGGAAATAAATTTAATTTTTAGAAGAAAAAAATTGAAATGATTAGCAAAGGAAACTAAATAAAATTTGAAAAGCGCCATTTTTTGTTGCAGGGACTTATGTGCTCAAGTATTATTATATATATACAACTATGGTGCACAGAAGAAAGAGGGCCAGTTTTTGTGACAATGTACAACCCACTTGGTTCCATTTTGGTTGCTCTTTTATCTTA

Coding sequence (CDS)

AATCCAAAAGGAATAGCAAAAGTAATGGGGACATTGGTTTCATTGGGTGGTGTGTTGATAATGACATTCTATAAAGGACCTATAATTAGAAATGTATGCCAACCTCTCATCCATATTCAACACAAAGCTACTTATCATCTCCATGAGGATTGGCTTAAGGGTTCACTTCTTACCGTTTCAAGCTGCCTTTCTTGGGCCATTTCCTACATTTTGCAGGCTTTTACGTTAAAGCGATACCCAGCACCGTTGTCGTTAACGACGTGGATGAACCTGTTTGGAGCAGTACAAACAGCTGTGTACACAGTGTCGACACAACACAAGGCAGGAGTTTGGAATATTGGATTCAACATTGACCTCTGGGCCATTATTTATGCAGGACTTATGTGCTCAAGTATTATTATATATATACAACTATGGTGCACAGAAGAAAGAGGGCCAGTTTTTGTGACAATGTACAACCCACTTGGTTCCATTTTGGTTGCTCTTTTATCT

Protein sequence

NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVSSCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLWAIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS

Homology

[Close]

BLAST of Cucsat.G3997 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 6.3e-29
Identity = 73/166 (43.98%), Postives = 106/166 (63.86%), Query Frame = 0


Query: 6   AKVMGTLVSLGGVLIMTFYKGPII------RNVCQPLIHIQHKATYHLHEDWLKGSLLTV 65
           AK++GTLV LGG L+MT YKGP+I       N+ Q   H  +      H +W+ G+LL +
Sbjct: 138 AKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNSQD---HNNWVVGTLLIL 197

Query: 66  SSCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQT-AVYTVSTQHKAGVWNIGFNID 125
             C++W+  Y+LQ+ T+K YPA LSL+  + L GAVQ+ AV  V  +H +G W +G++  
Sbjct: 198 LGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVERHPSG-WAVGWDAR 257

Query: 126 LWAIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 165
           L+A +Y G++ S I  Y+Q    + RGPVFVT +NPL  ILVAL++
Sbjct: 258 LFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIA 299

[Close]

BLAST of Cucsat.G3997 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.1e-25
Identity = 64/160 (40.00%), Postives = 96/160 (60.00%), Query Frame = 0


Query: 5   IAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVSSCLS 64
           + KVMGTLV++ G ++M FYKGP I      L       T     D+LK ++  + + LS
Sbjct: 135 LVKVMGTLVTVVGSILMIFYKGPFINFFRSHLTAASSPPT----ADYLKAAVFLLLASLS 194

Query: 65  WAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLWAIIY 124
           WA  ++LQA TLK+Y A LS++T +   G +Q+       +H     NIGF+++L A  Y
Sbjct: 195 WASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNIGFDMNLLASAY 254

Query: 125 AGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 165
           AG+M SSI  Y+Q    + +GPVFVT +NPL  ++V+++S
Sbjct: 255 AGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMS 290

[Close]

BLAST of Cucsat.G3997 vs. ExPASy Swiss-Prot
Match: Q501F8 (WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.5e-25
Identity = 59/165 (35.76%), Postives = 92/165 (55.76%), Query Frame = 0


Query: 3   KGIAKVMGTLVSLGGVLIMTFYKGPIIR--NVCQPLIHIQHKATYH--LHEDWLKGSLLT 62
           + +AKV+GT +++GG ++MT YKGP I         +H     T      ++W+ G+L  
Sbjct: 133 RSLAKVIGTAITVGGAMVMTLYKGPAIELFKTAHSSLHGGSSGTSSETTDQNWVTGTLAV 192

Query: 63  VSSCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNID 122
           + S  +WA  +ILQ+FTLK+YPA LSL  W+   G V   + ++        W +G +  
Sbjct: 193 MGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKVGMDSG 252

Query: 123 LWAIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALL 164
             A +Y+G++CS +  YIQ     ERGPVF T ++P+  I+ A L
Sbjct: 253 TLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFL 297

[Close]

BLAST of Cucsat.G3997 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.5e-25
Identity = 63/171 (36.84%), Postives = 97/171 (56.73%), Query Frame = 0


Query: 3   KGIAKVMGTLVSLGGVLIMTFYKGPIIRNV--------CQPLIHIQH--KATYHLHEDWL 62
           + IAKV+GT++++ G L+MT YKGPI+  +                H       + + W+
Sbjct: 133 RSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGAAAMDKHWI 192

Query: 63  KGSLLTVSSCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWN 122
            G+L+ +     WA  +ILQ+FTLK+YPA LSLTT + L G ++    ++ T      W 
Sbjct: 193 PGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVTVRDLSAWK 252

Query: 123 IGFNIDLWAIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALL 164
           IGF+ +L+A  Y+G++CS +  Y+Q     ERGPVFV  +NPL  ++ A L
Sbjct: 253 IGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAAL 303

[Close]

BLAST of Cucsat.G3997 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.2e-25
Identity = 63/163 (38.65%), Postives = 96/163 (58.90%), Query Frame = 0


Query: 3   KGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVSSC 62
           +   KV+GTL ++GG +IMT  KGP++       +   + A   +H   +KG++L    C
Sbjct: 137 RSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNTAGTDIHSA-IKGAVLVTIGC 196

Query: 63  LSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQ-TAVYTVSTQHKAGVWNIGFNIDLWA 122
            S+A   ILQA TL+ YPA LSLT W+ L G ++ TAV  V  +     W IG++  L  
Sbjct: 197 FSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGWDTKLLT 256

Query: 123 IIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 165
             Y+G++CS++  Y+     + RGPVFVT ++PL  I+VA++S
Sbjct: 257 ATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMS 298

[Close]

BLAST of Cucsat.G3997 vs. NCBI nr
Match: XP_004142259.1 (WAT1-related protein At4g08300 [Cucumis sativus] >KAE8651819.1 hypothetical protein Csa_006157 [Cucumis sativus])

HSP 1 Score: 341 bits (874), Expect = 2.18e-115
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 60
           NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS
Sbjct: 136 NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 195

Query: 61  SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 120
           SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW
Sbjct: 196 SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 255

Query: 121 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 164
           AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS
Sbjct: 256 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 299

[Close]

BLAST of Cucsat.G3997 vs. NCBI nr
Match: XP_008460633.2 (PREDICTED: LOW QUALITY PROTEIN: WAT1-related protein At4g08300-like [Cucumis melo])

HSP 1 Score: 313 bits (801), Expect = 2.65e-104
Identity = 148/164 (90.24%), Postives = 156/164 (95.12%), Query Frame = 0


Query: 1   NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 60
           NPKGIAKVMGTLVSLGGVLIMTFYKGPII++VC PLIHIQHKATY+LHEDWLKGSLLTVS
Sbjct: 137 NPKGIAKVMGTLVSLGGVLIMTFYKGPIIKSVCHPLIHIQHKATYYLHEDWLKGSLLTVS 196

Query: 61  SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 120
           SCLSWAISYILQAFTLKRYPAPLSLTTWMN+FGA QTA YTV TQ KAGVWN+G NIDLW
Sbjct: 197 SCLSWAISYILQAFTLKRYPAPLSLTTWMNMFGAAQTAFYTVLTQRKAGVWNLGLNIDLW 256

Query: 121 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 164
           AIIYAG+MCS IIIYIQLWCTEE+GPVFVTMYNPL SILVALL+
Sbjct: 257 AIIYAGIMCSGIIIYIQLWCTEEKGPVFVTMYNPLCSILVALLA 300

[Close]

BLAST of Cucsat.G3997 vs. NCBI nr
Match: XP_038906925.1 (WAT1-related protein At5g07050-like [Benincasa hispida])

HSP 1 Score: 285 bits (730), Expect = 8.03e-94
Identity = 134/164 (81.71%), Postives = 149/164 (90.85%), Query Frame = 0


Query: 1   NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 60
           +PKGIAKVMGTLVSLGGV+IMTFYKGPIIRN+  P IHIQH+AT + HEDWLKGSLLTVS
Sbjct: 137 HPKGIAKVMGTLVSLGGVMIMTFYKGPIIRNIWHPFIHIQHRAT-NFHEDWLKGSLLTVS 196

Query: 61  SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 120
           SCLSWAISY++QAFTLKRYPA LSLT WMNL GA Q  VYTV TQHK GVW++GFNIDLW
Sbjct: 197 SCLSWAISYVMQAFTLKRYPAQLSLTMWMNLIGAAQAGVYTVLTQHKPGVWSVGFNIDLW 256

Query: 121 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 164
           AIIYAG++CS +IIYIQLWCTEE+GPVFVTMYNPL +ILVALL+
Sbjct: 257 AIIYAGIVCSGLIIYIQLWCTEEKGPVFVTMYNPLCTILVALLA 299

[Close]

BLAST of Cucsat.G3997 vs. NCBI nr
Match: XP_022958777.1 (WAT1-related protein At4g08300-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 274 bits (701), Expect = 8.52e-90
Identity = 120/164 (73.17%), Postives = 148/164 (90.24%), Query Frame = 0


Query: 1   NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 60
           NP+GIAKV+GTLVSLGG ++MTFYKGPIIRN+  PLIH+QH AT+H+HE+WLKGS+LTVS
Sbjct: 98  NPRGIAKVLGTLVSLGGAMVMTFYKGPIIRNLWHPLIHMQHTATHHVHENWLKGSVLTVS 157

Query: 61  SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 120
           SC+SWA+SYI+QAFTLKRYPA LSLTTWMNL GA QT V+ + TQHK GVW +G NIDLW
Sbjct: 158 SCISWALSYIMQAFTLKRYPAQLSLTTWMNLIGAAQTGVFALLTQHKPGVWKVGINIDLW 217

Query: 121 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 164
            IIYAG++ S++++YIQLWCTE++GPVF TMY+PLG++LVALL+
Sbjct: 218 CIIYAGIVGSALVVYIQLWCTEKKGPVFATMYSPLGAVLVALLA 261

[Close]

BLAST of Cucsat.G3997 vs. NCBI nr
Match: KAG6574923.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 274 bits (701), Expect = 8.52e-90
Identity = 120/164 (73.17%), Postives = 148/164 (90.24%), Query Frame = 0


Query: 1   NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 60
           NP+GIAKV+GTLVSLGG ++MTFYKGPIIRN+  PLIH+QH AT+H+HE+WLKGS+LTVS
Sbjct: 98  NPRGIAKVLGTLVSLGGAMVMTFYKGPIIRNLWHPLIHMQHTATHHVHENWLKGSVLTVS 157

Query: 61  SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 120
           SC+SWA+SYI+QAFTLKRYPA LSLTTWMNL GA QT V+ + TQHK GVW +G NIDLW
Sbjct: 158 SCISWALSYIMQAFTLKRYPAQLSLTTWMNLIGAAQTGVFALLTQHKPGVWKVGINIDLW 217

Query: 121 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 164
            IIYAG++ S++++YIQLWCTE++GPVF TMY+PLG++LVALL+
Sbjct: 218 CIIYAGIVGSALVVYIQLWCTEKKGPVFATMYSPLGAVLVALLA 261

[Close]

BLAST of Cucsat.G3997 vs. ExPASy TrEMBL
Match: A0A0A0LLJ5 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_2G179220 PE=3 SV=1)

HSP 1 Score: 341 bits (874), Expect = 2.24e-115
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 60
           NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS
Sbjct: 159 NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 218

Query: 61  SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 120
           SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW
Sbjct: 219 SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 278

Query: 121 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 164
           AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS
Sbjct: 279 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 322

[Close]

BLAST of Cucsat.G3997 vs. ExPASy TrEMBL
Match: A0A1S3CCF6 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103499410 PE=3 SV=1)

HSP 1 Score: 313 bits (801), Expect = 1.28e-104
Identity = 148/164 (90.24%), Postives = 156/164 (95.12%), Query Frame = 0


Query: 1   NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 60
           NPKGIAKVMGTLVSLGGVLIMTFYKGPII++VC PLIHIQHKATY+LHEDWLKGSLLTVS
Sbjct: 137 NPKGIAKVMGTLVSLGGVLIMTFYKGPIIKSVCHPLIHIQHKATYYLHEDWLKGSLLTVS 196

Query: 61  SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 120
           SCLSWAISYILQAFTLKRYPAPLSLTTWMN+FGA QTA YTV TQ KAGVWN+G NIDLW
Sbjct: 197 SCLSWAISYILQAFTLKRYPAPLSLTTWMNMFGAAQTAFYTVLTQRKAGVWNLGLNIDLW 256

Query: 121 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 164
           AIIYAG+MCS IIIYIQLWCTEE+GPVFVTMYNPL SILVALL+
Sbjct: 257 AIIYAGIMCSGIIIYIQLWCTEEKGPVFVTMYNPLCSILVALLA 300

[Close]

BLAST of Cucsat.G3997 vs. ExPASy TrEMBL
Match: A0A6J1H2S1 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111459947 PE=3 SV=1)

HSP 1 Score: 274 bits (701), Expect = 4.13e-90
Identity = 120/164 (73.17%), Postives = 148/164 (90.24%), Query Frame = 0


Query: 1   NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 60
           NP+GIAKV+GTLVSLGG ++MTFYKGPIIRN+  PLIH+QH AT+H+HE+WLKGS+LTVS
Sbjct: 98  NPRGIAKVLGTLVSLGGAMVMTFYKGPIIRNLWHPLIHMQHTATHHVHENWLKGSVLTVS 157

Query: 61  SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 120
           SC+SWA+SYI+QAFTLKRYPA LSLTTWMNL GA QT V+ + TQHK GVW +G NIDLW
Sbjct: 158 SCISWALSYIMQAFTLKRYPAQLSLTTWMNLIGAAQTGVFALLTQHKPGVWKVGINIDLW 217

Query: 121 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 164
            IIYAG++ S++++YIQLWCTE++GPVF TMY+PLG++LVALL+
Sbjct: 218 CIIYAGIVGSALVVYIQLWCTEKKGPVFATMYSPLGAVLVALLA 261

[Close]

BLAST of Cucsat.G3997 vs. ExPASy TrEMBL
Match: A0A6J1H306 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111459947 PE=3 SV=1)

HSP 1 Score: 274 bits (701), Expect = 7.32e-90
Identity = 120/164 (73.17%), Postives = 148/164 (90.24%), Query Frame = 0


Query: 1   NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 60
           NP+GIAKV+GTLVSLGG ++MTFYKGPIIRN+  PLIH+QH AT+H+HE+WLKGS+LTVS
Sbjct: 116 NPRGIAKVLGTLVSLGGAMVMTFYKGPIIRNLWHPLIHMQHTATHHVHENWLKGSVLTVS 175

Query: 61  SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 120
           SC+SWA+SYI+QAFTLKRYPA LSLTTWMNL GA QT V+ + TQHK GVW +G NIDLW
Sbjct: 176 SCISWALSYIMQAFTLKRYPAQLSLTTWMNLIGAAQTGVFALLTQHKPGVWKVGINIDLW 235

Query: 121 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 164
            IIYAG++ S++++YIQLWCTE++GPVF TMY+PLG++LVALL+
Sbjct: 236 CIIYAGIVGSALVVYIQLWCTEKKGPVFATMYSPLGAVLVALLA 279

[Close]

BLAST of Cucsat.G3997 vs. ExPASy TrEMBL
Match: A0A6J1H4F2 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111459947 PE=3 SV=1)

HSP 1 Score: 274 bits (701), Expect = 1.41e-89
Identity = 120/164 (73.17%), Postives = 148/164 (90.24%), Query Frame = 0


Query: 1   NPKGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVS 60
           NP+GIAKV+GTLVSLGG ++MTFYKGPIIRN+  PLIH+QH AT+H+HE+WLKGS+LTVS
Sbjct: 137 NPRGIAKVLGTLVSLGGAMVMTFYKGPIIRNLWHPLIHMQHTATHHVHENWLKGSVLTVS 196

Query: 61  SCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLW 120
           SC+SWA+SYI+QAFTLKRYPA LSLTTWMNL GA QT V+ + TQHK GVW +G NIDLW
Sbjct: 197 SCISWALSYIMQAFTLKRYPAQLSLTTWMNLIGAAQTGVFALLTQHKPGVWKVGINIDLW 256

Query: 121 AIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 164
            IIYAG++ S++++YIQLWCTE++GPVF TMY+PLG++LVALL+
Sbjct: 257 CIIYAGIVGSALVVYIQLWCTEKKGPVFATMYSPLGAVLVALLA 300

[Close]

BLAST of Cucsat.G3997 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 128.6 bits (322), Expect = 4.5e-30
Identity = 73/166 (43.98%), Postives = 106/166 (63.86%), Query Frame = 0


Query: 6   AKVMGTLVSLGGVLIMTFYKGPII------RNVCQPLIHIQHKATYHLHEDWLKGSLLTV 65
           AK++GTLV LGG L+MT YKGP+I       N+ Q   H  +      H +W+ G+LL +
Sbjct: 138 AKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNSQD---HNNWVVGTLLIL 197

Query: 66  SSCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQT-AVYTVSTQHKAGVWNIGFNID 125
             C++W+  Y+LQ+ T+K YPA LSL+  + L GAVQ+ AV  V  +H +G W +G++  
Sbjct: 198 LGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVERHPSG-WAVGWDAR 257

Query: 126 LWAIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 165
           L+A +Y G++ S I  Y+Q    + RGPVFVT +NPL  ILVAL++
Sbjct: 258 LFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIA 299

[Close]

BLAST of Cucsat.G3997 vs. TAIR 10
Match: AT2G40900.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 117.9 bits (294), Expect = 7.9e-27
Identity = 64/160 (40.00%), Postives = 96/160 (60.00%), Query Frame = 0


Query: 5   IAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVSSCLS 64
           + KVMGTLV++ G ++M FYKGP I      L       T     D+LK ++  + + LS
Sbjct: 135 LVKVMGTLVTVVGSILMIFYKGPFINFFRSHLTAASSPPT----ADYLKAAVFLLLASLS 194

Query: 65  WAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNIDLWAIIY 124
           WA  ++LQA TLK+Y A LS++T +   G +Q+       +H     NIGF+++L A  Y
Sbjct: 195 WASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNIGFDMNLLASAY 254

Query: 125 AGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 165
           AG+M SSI  Y+Q    + +GPVFVT +NPL  ++V+++S
Sbjct: 255 AGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMS 290

[Close]

BLAST of Cucsat.G3997 vs. TAIR 10
Match: AT4G08300.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.0e-26
Identity = 59/165 (35.76%), Postives = 92/165 (55.76%), Query Frame = 0


Query: 3   KGIAKVMGTLVSLGGVLIMTFYKGPIIR--NVCQPLIHIQHKATYH--LHEDWLKGSLLT 62
           + +AKV+GT +++GG ++MT YKGP I         +H     T      ++W+ G+L  
Sbjct: 133 RSLAKVIGTAITVGGAMVMTLYKGPAIELFKTAHSSLHGGSSGTSSETTDQNWVTGTLAV 192

Query: 63  VSSCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWNIGFNID 122
           + S  +WA  +ILQ+FTLK+YPA LSL  W+   G V   + ++        W +G +  
Sbjct: 193 MGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKVGMDSG 252

Query: 123 LWAIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALL 164
             A +Y+G++CS +  YIQ     ERGPVF T ++P+  I+ A L
Sbjct: 253 TLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFL 297

[Close]

BLAST of Cucsat.G3997 vs. TAIR 10
Match: AT1G21890.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 116.7 bits (291), Expect = 1.8e-26
Identity = 63/171 (36.84%), Postives = 97/171 (56.73%), Query Frame = 0


Query: 3   KGIAKVMGTLVSLGGVLIMTFYKGPIIRNV--------CQPLIHIQH--KATYHLHEDWL 62
           + IAKV+GT++++ G L+MT YKGPI+  +                H       + + W+
Sbjct: 133 RSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGAAAMDKHWI 192

Query: 63  KGSLLTVSSCLSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQTAVYTVSTQHKAGVWN 122
            G+L+ +     WA  +ILQ+FTLK+YPA LSLTT + L G ++    ++ T      W 
Sbjct: 193 PGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVTVRDLSAWK 252

Query: 123 IGFNIDLWAIIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALL 164
           IGF+ +L+A  Y+G++CS +  Y+Q     ERGPVFV  +NPL  ++ A L
Sbjct: 253 IGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAAL 303

[Close]

BLAST of Cucsat.G3997 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 116.3 bits (290), Expect = 2.3e-26
Identity = 63/163 (38.65%), Postives = 96/163 (58.90%), Query Frame = 0


Query: 3   KGIAKVMGTLVSLGGVLIMTFYKGPIIRNVCQPLIHIQHKATYHLHEDWLKGSLLTVSSC 62
           +   KV+GTL ++GG +IMT  KGP++       +   + A   +H   +KG++L    C
Sbjct: 137 RSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNTAGTDIHSA-IKGAVLVTIGC 196

Query: 63  LSWAISYILQAFTLKRYPAPLSLTTWMNLFGAVQ-TAVYTVSTQHKAGVWNIGFNIDLWA 122
            S+A   ILQA TL+ YPA LSLT W+ L G ++ TAV  V  +     W IG++  L  
Sbjct: 197 FSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGWDTKLLT 256

Query: 123 IIYAGLMCSSIIIYIQLWCTEERGPVFVTMYNPLGSILVALLS 165
             Y+G++CS++  Y+     + RGPVFVT ++PL  I+VA++S
Sbjct: 257 ATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMS 298

The following BLAST results are available for this feature:

BLAST of Cucsat.G3997 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SUF1	6.3e-29	43.98	WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2...	[more]
F4IJ08	1.1e-25	40.00	WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2...	[more]
Q501F8	1.5e-25	35.76	WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2...	[more]
F4HZQ7	2.5e-25	36.84	WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2...	[more]
Q9ZUS1	3.2e-25	38.65	WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2...	[more]

BLAST of Cucsat.G3997 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142259.1	2.18e-115	100.00	WAT1-related protein At4g08300 [Cucumis sativus] >KAE8651819.1 hypothetical prot...	[more]
XP_008460633.2	2.65e-104	90.24	PREDICTED: LOW QUALITY PROTEIN: WAT1-related protein At4g08300-like [Cucumis mel...	[more]
XP_038906925.1	8.03e-94	81.71	WAT1-related protein At5g07050-like [Benincasa hispida]	[more]
XP_022958777.1	8.52e-90	73.17	WAT1-related protein At4g08300-like isoform X3 [Cucurbita moschata]	[more]
KAG6574923.1	8.52e-90	73.17	WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cucsat.G3997 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LLJ5	2.24e-115	100.00	WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_2G179220 PE=3 SV=1	[more]
A0A1S3CCF6	1.28e-104	90.24	WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103499410 PE=3 SV=1	[more]
A0A6J1H2S1	4.13e-90	73.17	WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111459947 PE=3 SV=1	[more]
A0A6J1H306	7.32e-90	73.17	WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111459947 PE=3 SV=1	[more]
A0A6J1H4F2	1.41e-89	73.17	WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111459947 PE=3 SV=1	[more]

BLAST of Cucsat.G3997 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G08290.1	4.5e-30	43.98	nodulin MtN21 /EamA-like transporter family protein	[more]
AT2G40900.1	7.9e-27	40.00	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G08300.1	1.0e-26	35.76	nodulin MtN21 /EamA-like transporter family protein	[more]
AT1G21890.1	1.8e-26	36.84	nodulin MtN21 /EamA-like transporter family protein	[more]
AT2G37460.1	2.3e-26	38.65	nodulin MtN21 /EamA-like transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 53..164 e-value: 6.3E-7 score: 29.6
None	No IPR available	PANTHER	PTHR31218:SF46	WAT1-RELATED PROTEIN	coord: 1..163
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 1..163

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G3997.T1	Cucsat.G3997.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity