Cucsat.G377 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G377
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	heavy metal-associated isoprenylated plant protein 3
Location	ctg1: 8063014 .. 8064615 (-)
RNA-Seq Expression	Cucsat.G377
Synteny	Cucsat.G377

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTAACCTTCTCTCTAAAGCTTTCCAAAGGCTGAATTTCAAATAGACAATCTTCAATTTCTACAAGTCCATAAACAAAAAGATGCAAGCAGTTAGGGAGAAGCTACACGATATGAGCGTCATGCGTAAGGCCAAGGCCGAGGCAAAGGCCGAAGAGAAGGTGAGCTCTCGATGATGTTAGTTGTATTATTGAAAACATCTTCAATTCGAAAATATCATGAATACTTATTAATTGTTTTATCTTCCATAACTAGTACTGTTTGTTTTTATCCCTTCCACTTGAGATATATAACAATCGAGATTCAAATAAAAATTTAATATCTACATCTCACATTCAAACTATCAAATTTTTTTTTATATAGAAATCATACATAATCTTCTTTTTTTTTATGTTTTCTAATTTTTTTATCAAATCCTTTCCCATATTTTTATTTTGTTCAATTATCTAAATTTCCTAATGAATTTACCACACATTCAAATGACTTTTTTAAATGGATCTCGGGTGATTCACCATGTAATTTCTGTACGATCTCAAGTCATCGTCCTAGTTGAGATCTCACCGAAAAAGCCCTAAAGAGTAGTCATGCTAGTTATGACTATAGGAAAATGTCTTGCCCGAACAAAGATATTTGAGATTTGAACGATGAAATACGAGTTTTCATTTTATTTTAATTGTAAATGGGCAGGCAGAGAAGGAGCTAGCAAAAGCAAGAGTGGAAGTGGCTCATGAGGTAAGATTGGCACGAGAGGCTGAAGCTGCGATGGATCTTCATGTAGCCAAAGCTGGAATGAAGGCTGAGAAAGAGATGGCTAAGTATGCTGATACTCACACCAATACCTTTGTGTCTCCTGGTGGAACCACCACCCTTCCTGATTACTCCGTACCAGTAGCTCCCGGCATGACTATGGTTGGAGCCCCTGGCACCGTCGACATGGCCGGAGCTCCTTCTCCCGTTAATAAGAAGTTACTGTAAAAAAAAGTTCTTGTGTAACGCCATGCATAAAAAGTTTCATCTTTTATTTGCAAATAATGTACTTTGATTTGTTTTCTTTTTTCTACAAAGTTCTATATTATTCTACCACATCTTTAGATGTTGTGTAGTATATATTATAATCAATACACACAATAGCATTTAAGATCCTATTTTCGTTTCATTTTTCAGGTTACTCGTGGCTCAAGAAATCATGAAGTGGGGGAATATAAATCTAATGGAGGGGAAATTAATTAACCAAATCCAACTTGTTTGTTACCCTAAAGTTCTTAAAAGTTAACACTATTAGAAATGGAAAAGAGTTGATATAATTTGGCATAACCAAAGACTAATATTGGATTAAAATAAAACAAAGAGAATCACCAAGGGCAGGCAGGCAGGTGAACAAACTCTCAGGAATTCTCACCAAAACGATGTCGTGAGGAAAATGTAAAACTCTTTAAAAATTATAAATAAAGTAAAGATCATAAATATTATATATATATATATATATATAATATTTCTTCTGCATATAATTTTGTTGTGTACACAAAAAATAATGGGAGAGAAAGAGAAGGTTGAAGGTATCACTGCCATTTACAAACTAAATCTTCATTGTCATCAATGTTGGAGAGATATCAAAAAACCTCTTTCCACAACACAAGGTAACATCATATATATGTATTTTCAATTAGTTTCCGTTCTTTGTTTTTAAAATTTATATTTCTTTCCCGCTATTAGTTAGTATCGAACTTTTTTTTACACGATAACTTAACTTTCAAAATGTTTTATATACATACGGGATGTTTATTTGGAGATATGACACTATTATTTCATCCTTCTATATCTTTGAAGTGCATGTATGTGTAAATTGTTCGAGTTGATTGGTTAATTAACTTTTTCTTTTTTAATTCATATTTATTGTTTGGGTTCATAATTTTTTTTTGTCTGGGTGGGTGAAATTAGGTGTTCAAAATGTTGAAGTTGATATGGAGAAAAATGAAATTAGGGTGAAGGGTTCAAATTTGGATGTGCTAAAAATTCAGAAGCAAATAGAGAAGTTGAGCAAGAAAAAAGTTGAATTGATTTCTCCCAAAGTGAAGCCCAAAGAGAAAGACCCTCCTAAACCCATTGATGACAAACCCAAACCAACCATTGTGAGTATAAATTCTTAAGCTTAATTATTTCTAATTGATTAAGATTATATGATTATTCTCAACCTTTATACACAAATTAAATGCTTTTTACGAACTATATATATACACACATGATATTTTGATGTCACATGATCATAGTGTTCATATCATTCTCAACACTCTCCATTAGGAAACTAATTTAGATTTCATCGTTCAAAGTAAAATTTTTATCATTATTGTTAGTTAATTGAGTATAAATTATATTAACCGTAAAACCTAATTTTATGCCATGCATAGAAAAGGCACTATGCGAAAATGTTTTTAATAATTACATGGAAAATGTTAATTTAGTAACATTTTTCCTAACAAAATTATTATCTTACCTTAAAAACGACCCTTATTTTCTAACTAAAATCTTATTAAATAACAATTTAGAGAAATTTGGTTAATACATAATGAAAAACGTAGATTTGAAGTTACAAATTTATAGTAAGATTCAATTGAAAAGATCCATCCATCAAGAGAAAGAAAATATTAATAAAATAACAAAATTAATTACATTAAATTAAATGGGGTTTTTCTTTTGATCCACTTTGGCAGGTCAATCGCATAATAACAGCTAAGGTTCACTTGCACTGCCCCAAATGCGAGCAAGACTTGAAGAACAAGTTGCTAAAGCATAAAGGTTTTTGTGGCACACTAATCCCTTTTTTTTAGTATTATTAAATAGGTTTTTCTTACAAAAATAATAATAATAATATGTTTTCTTTCCAAATTCTATTTATTTTATTTTTATAGCTTTTGCTTTAATCTATCATTTTCTTGTAACAAATCTATTTTAATCTCTACCTACTAGAAATATATCCTATTAGATATCACTTGAAGTTGTCATTTTTGGTCACCAGGACTTAAACTCGAAATTATTATATAGAATATGGTGTTCAAAGATTCAAATAGGAAATTTTTCATAACTTGAGATGATGATACAATTATAAAGTTTGAATACTATTTCTCTTGTTTTACTACACTTAGCAGCATGAGTTCAGATGGGGTCTATTGCAAAATATTCACGAAAAATTATTCCCCATTTTGAAGAACAATAATGATGATTTTTAATATTGGCAGGAATATATAGTGTGAAAACAGACATAAAAGCTCAAACCCTAACAATGGAAGGAAGCATTGAAGCAGAGAAGTTCAAATCCTATTTGAAGAATAAGCTACAGAAACATGTAGATATTACAGTGGATACGAAATCGACTGATTCATCGAAGTCGACGGCGGTAGCATCAGAGAAGAAGAAAGAAAGTAATACTGATAAAAAAGAGAAGCCAAAAGAAAAGGCTTCATCTGAAACAACAATAACTACTACTGACAAGAAGACTATTGTTGTTGCAGAGATTCAAAGTAAAGAGAATAATTCTAATGATATTAATAATAAAAATAATAATGTCCCTTATTTCATTCACTATGTCTATGCTCCTCAATTGTTTAGTGATGAAAAT

Coding sequence (CDS)

ATGGGAGAGAAAGAGAAGGTTGAAGGTATCACTGCCATTTACAAACTAAATCTTCATTGTCATCAATGTTGGAGAGATATCAAAAAACCTCTTTCCACAACACAAGGTGTTCAAAATGTTGAAGTTGATATGGAGAAAAATGAAATTAGGGTGAAGGGTTCAAATTTGGATGTGCTAAAAATTCAGAAGCAAATAGAGAAGTTGAGCAAGAAAAAAGTTGAATTGATTTCTCCCAAAGTGAAGCCCAAAGAGAAAGACCCTCCTAAACCCATTGATGACAAACCCAAACCAACCATTGTCAATCGCATAATAACAGCTAAGGTTCACTTGCACTGCCCCAAATGCGAGCAAGACTTGAAGAACAAGTTGCTAAAGCATAAAGGAATATATAGTGTGAAAACAGACATAAAAGCTCAAACCCTAACAATGGAAGGAAGCATTGAAGCAGAGAAGTTCAAATCCTATTTGAAGAATAAGCTACAGAAACATGTAGATATTACAGTGGATACGAAATCGACTGATTCATCGAAGTCGACGGCGGTAGCATCAGAGAAGAAGAAAGAAAGTAATACTGATAAAAAAGAGAAGCCAAAAGAAAAGGCTTCATCTGAAACAACAATAACTACTACTGACAAGAAGACTATTGTTGTTGCAGAGATTCAAAGTAAAGAGAATAATTCTAATGATATTAATAATAAAAATAATAATGTCCCTTATTTCATTCACTATGTCTATGCTCCTCAATTGTTTAGTGATGAAAAT

Protein sequence

MGEKEKVEGITAIYKLNLHCHQCWRDIKKPLSTTQGVQNVEVDMEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRIITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKHVDITVDTKSTDSSKSTAVASEKKKESNTDKKEKPKEKASSETTITTTDKKTIVVAEIQSKENNSNDINNKNNNVPYFIHYVYAPQLFSDEN

Homology

[Close]

BLAST of Cucsat.G377 vs. ExPASy Swiss-Prot
Match: Q9SHQ8 (Heavy metal-associated isoprenylated plant protein 4 OS=Arabidopsis thaliana OX=3702 GN=HIPP04 PE=3 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 1.9e-23
Identity = 69/141 (48.94%), Postives = 96/141 (68.09%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEK----DPPKPIDDKPKPTI 60
           +EKNEI+VKG  ++V+KI KQIEK SKKKVELISPK    +K         + +K    I
Sbjct: 49  LEKNEIKVKG-KIEVVKIHKQIEKWSKKKVELISPKPSEVKKTTTTTTTTSVVEKKTTEI 108

Query: 61  ---VNRIITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYL 120
              V R    KVH+HC +C++DL++KLLKHK I+ VKTD KAQTLT++G+IE+ K  +Y+
Sbjct: 109 KKDVIRTTVLKVHIHCAQCDKDLQHKLLKHKAIHIVKTDTKAQTLTVQGTIESAKLLAYI 168

Query: 121 KNKLQKHVDITVDTKSTDSSK 135
           K K+ KH +I + +K+ +  K
Sbjct: 169 KKKVHKHAEI-ISSKTEEEKK 187

[Close]

BLAST of Cucsat.G377 vs. ExPASy Swiss-Prot
Match: Q9ZUV1 (Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=3702 GN=HIPP01 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.9e-10
Identity = 44/137 (32.12%), Postives = 76/137 (55.47%), Query Frame = 0


Query: 2   EKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKD-PPKPIDDKPKPTIVNRI 61
           + N++ + G  +D +KI ++++K SKKKVELISPK K   K+   K  +DK +  +    
Sbjct: 64  DSNKLTLIGFIMDPVKIAEKLQKKSKKKVELISPKPKKDTKENNEKKANDKTQTVVAVTT 123

Query: 62  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 121
           +  KV+  C  C + ++  +   KG+Y VK D + +T+T+ G+++ +     LK KL+K 
Sbjct: 124 VVLKVNCSCDGCIKRIQKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKT 183

Query: 122 VDITVDTKSTDSSKSTA 138
           V +  + K     K  A
Sbjct: 184 VQVVPEKKKKKKDKDNA 200

[Close]

BLAST of Cucsat.G377 vs. ExPASy Swiss-Prot
Match: Q9C5D3 (Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=3702 GN=HIPP07 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.2e-10
Identity = 37/126 (29.37%), Postives = 72/126 (57.14%), Query Frame = 0


Query: 5   EIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKP-------KPTIV 64
           ++ VKG   D LK+  ++++ + ++V+L+SP   P      K  +DKP       +P +V
Sbjct: 111 KVVVKGEKADPLKVLARVQRKTHRQVQLLSPIPPPPPPPEKKAEEDKPIVEEKKVEPPVV 170

Query: 65  NRIITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKL 124
             ++  KVH+HC  C  ++K ++++ KG+ S ++D+K+  +T++G  E +K   Y+  + 
Sbjct: 171 VTVV-LKVHMHCEACATEIKKRIMRMKGVESAESDLKSSQVTVKGVFEPQKLVEYVYKRT 230

[Close]

BLAST of Cucsat.G377 vs. ExPASy Swiss-Prot
Match: Q9M8T7 (Heavy metal-associated isoprenylated plant protein 8 OS=Arabidopsis thaliana OX=3702 GN=HIPP08 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 2.5e-07
Identity = 40/133 (30.08%), Postives = 69/133 (51.88%), Query Frame = 0


Query: 4   NEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRIITA 63
           N++ V G   D LKI ++++K   +  E+ISPK  PK+         +  P I   I+  
Sbjct: 73  NKVVVSGKFDDPLKILRRVQKKFSRNAEMISPKHNPKQDQKEPQQKKESAPEIKTAIL-- 132

Query: 64  KVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKHVDI 123
           ++++HC  C  ++K  + K KGI SV+ D    T+ + G ++  K    +K KL KH ++
Sbjct: 133 RMNMHCEGCVHEIKRGIEKIKGIQSVEPDRSKSTVVVRGVMDPPKLVEKIKKKLGKHAEL 192

Query: 124 --TVDTKSTDSSK 135
              +  K  D++K
Sbjct: 193 LSQITEKGKDNNK 203

[Close]

BLAST of Cucsat.G377 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 3.3e-07
Identity = 41/136 (30.15%), Postives = 70/136 (51.47%), Query Frame = 0


Query: 13  LDVLKIQKQIEKLSKKKVELISP-----------------KVKPKEKDPPKPIDDKPKPT 72
           LD +K+++++E+ +KKKV+L+SP                 K K +EK  P   D KPK T
Sbjct: 71  LDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKET 130

Query: 73  IVNRIITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKN 132
            V   +  K++ HC  C   ++  + K KG+  +  D +   LT++G+++ +K    L  
Sbjct: 131 PVTTAV-LKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSE 190

[Close]

BLAST of Cucsat.G377 vs. NCBI nr
Match: XP_004134354.1 (heavy metal-associated isoprenylated plant protein 4 [Cucumis sativus] >KAE8650278.1 hypothetical protein Csa_010815 [Cucumis sativus])

HSP 1 Score: 257 bits (657), Expect = 2.64e-84
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60
           MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI
Sbjct: 44  MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 103

Query: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120
           ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH
Sbjct: 104 ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 163

Query: 121 VDITVDTKSTDSSKSTA 137
           VDITVDTKSTDSSKSTA
Sbjct: 164 VDITVDTKSTDSSKSTA 180

[Close]

BLAST of Cucsat.G377 vs. NCBI nr
Match: XP_008438227.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 3 [Cucumis melo])

HSP 1 Score: 244 bits (624), Expect = 2.56e-79
Identity = 133/138 (96.38%), Postives = 134/138 (97.10%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60
           MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPK+KDPPKP  DKPKPTIVNR 
Sbjct: 45  MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKDKDPPKPTADKPKPTIVNRT 104

Query: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120
           ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH
Sbjct: 105 ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 164

Query: 121 VDITVDTKSTD-SSKSTA 137
           VDITVDTKSTD SSKSTA
Sbjct: 165 VDITVDTKSTDYSSKSTA 182

[Close]

BLAST of Cucsat.G377 vs. NCBI nr
Match: XP_038878052.1 (heavy metal-associated isoprenylated plant protein 4 [Benincasa hispida])

HSP 1 Score: 224 bits (571), Expect = 2.61e-71
Identity = 120/136 (88.24%), Postives = 125/136 (91.91%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60
           MEKNEIRVKGSNLDVLKI+KQIEKLSKK+VELISPKVKPKEKDPPKP  +KPKPTIV+R 
Sbjct: 42  MEKNEIRVKGSNLDVLKIEKQIEKLSKKRVELISPKVKPKEKDPPKPPHNKPKPTIVHRT 101

Query: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120
           ITAKVHLHC KCEQDLKNKLLKHKGIYSVKTD+KAQTLTMEGSIEAEKFKSYLK KLQKH
Sbjct: 102 ITAKVHLHCAKCEQDLKNKLLKHKGIYSVKTDMKAQTLTMEGSIEAEKFKSYLKKKLQKH 161

Query: 121 VDITVDTKSTDSSKST 136
            DIT DTK  DS K T
Sbjct: 162 ADITADTKLADSLKPT 177

[Close]

BLAST of Cucsat.G377 vs. NCBI nr
Match: XP_022147087.1 (heavy metal-associated isoprenylated plant protein 4 [Momordica charantia])

HSP 1 Score: 206 bits (525), Expect = 1.95e-64
Identity = 108/134 (80.60%), Postives = 119/134 (88.81%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60
           MEKNEI+VKGSNLDVLK  KQIEKLSKKKVELISPKV+P EK+ PKP D+KPK  +++R 
Sbjct: 45  MEKNEIKVKGSNLDVLKXHKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRT 104

Query: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120
           ITAKVHLHC KCEQDLKNKLLKHKGIYSVKTDIKAQTLT+EG+IE EKF+SYLK KL KH
Sbjct: 105 ITAKVHLHCAKCEQDLKNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKH 164

Query: 121 VDITVDTKSTDSSK 134
           +DIT D KS DSSK
Sbjct: 165 IDITADKKSVDSSK 178

[Close]

BLAST of Cucsat.G377 vs. NCBI nr
Match: XP_023528992.1 (heavy metal-associated isoprenylated plant protein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 202 bits (515), Expect = 5.49e-63
Identity = 108/134 (80.60%), Postives = 120/134 (89.55%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60
           MEKNEIRVKGSNLDVLKI KQIEKLSKK+V+LISPKVKPKEK+ PKP D+KPKP++++R 
Sbjct: 42  MEKNEIRVKGSNLDVLKIHKQIEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIHRT 101

Query: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120
           ITAKVHLHC KCE+DLKN LLKHKGIYSVKTD+KAQTLTMEG+IE EKFKSYLK KLQKH
Sbjct: 102 ITAKVHLHCAKCEKDLKNNLLKHKGIYSVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKH 161

Query: 121 VDITVDTKSTDSSK 134
            DIT    + DSSK
Sbjct: 162 ADITT---AVDSSK 172

[Close]

BLAST of Cucsat.G377 vs. ExPASy TrEMBL
Match: A0A0A0L4G4 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G127060 PE=3 SV=1)

HSP 1 Score: 257 bits (657), Expect = 3.08e-85
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60
           MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI
Sbjct: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60

Query: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120
           ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH
Sbjct: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120

Query: 121 VDITVDTKSTDSSKSTA 137
           VDITVDTKSTDSSKSTA
Sbjct: 121 VDITVDTKSTDSSKSTA 137

[Close]

BLAST of Cucsat.G377 vs. ExPASy TrEMBL
Match: A0A1S3AVV8 (heavy metal-associated isoprenylated plant protein 3 OS=Cucumis melo OX=3656 GN=LOC103483396 PE=3 SV=1)

HSP 1 Score: 244 bits (624), Expect = 1.24e-79
Identity = 133/138 (96.38%), Postives = 134/138 (97.10%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60
           MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPK+KDPPKP  DKPKPTIVNR 
Sbjct: 45  MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKDKDPPKPTADKPKPTIVNRT 104

Query: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120
           ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH
Sbjct: 105 ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 164

Query: 121 VDITVDTKSTD-SSKSTA 137
           VDITVDTKSTD SSKSTA
Sbjct: 165 VDITVDTKSTDYSSKSTA 182

[Close]

BLAST of Cucsat.G377 vs. ExPASy TrEMBL
Match: A0A6J1D1E3 (heavy metal-associated isoprenylated plant protein 4 OS=Momordica charantia OX=3673 GN=LOC111016109 PE=3 SV=1)

HSP 1 Score: 206 bits (525), Expect = 9.44e-65
Identity = 108/134 (80.60%), Postives = 119/134 (88.81%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60
           MEKNEI+VKGSNLDVLK  KQIEKLSKKKVELISPKV+P EK+ PKP D+KPK  +++R 
Sbjct: 45  MEKNEIKVKGSNLDVLKXHKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRT 104

Query: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120
           ITAKVHLHC KCEQDLKNKLLKHKGIYSVKTDIKAQTLT+EG+IE EKF+SYLK KL KH
Sbjct: 105 ITAKVHLHCAKCEQDLKNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKH 164

Query: 121 VDITVDTKSTDSSK 134
           +DIT D KS DSSK
Sbjct: 165 IDITADKKSVDSSK 178

[Close]

BLAST of Cucsat.G377 vs. ExPASy TrEMBL
Match: A0A6J1IZ45 (heavy metal-associated isoprenylated plant protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111479765 PE=3 SV=1)

HSP 1 Score: 202 bits (513), Expect = 5.69e-63
Identity = 107/134 (79.85%), Postives = 120/134 (89.55%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60
           MEKNEIRVKGSNLDVLKI KQIEKLSKK+V+LISPKVKPKEK+ PKP D+KPKP++++R 
Sbjct: 42  MEKNEIRVKGSNLDVLKIHKQIEKLSKKRVDLISPKVKPKEKETPKPTDNKPKPSLIHRT 101

Query: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120
           ITAKVHLHC KCE+DLKN LLKHKGIYSVKTD+KAQTLT+EG+IE EKFKSYLK KLQKH
Sbjct: 102 ITAKVHLHCAKCEKDLKNNLLKHKGIYSVKTDMKAQTLTIEGTIEPEKFKSYLKKKLQKH 161

Query: 121 VDITVDTKSTDSSK 134
            DIT    + DSSK
Sbjct: 162 ADITT---AADSSK 172

[Close]

BLAST of Cucsat.G377 vs. ExPASy TrEMBL
Match: A0A6J1EFL8 (heavy metal-associated isoprenylated plant protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111432082 PE=3 SV=1)

HSP 1 Score: 201 bits (511), Expect = 1.08e-62
Identity = 107/134 (79.85%), Postives = 119/134 (88.81%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60
           MEKNEIRVKGSNLDVLKI KQIEKLSKK+V+LISPKVKPKEK+ PKP D+KPKP+++ R 
Sbjct: 42  MEKNEIRVKGSNLDVLKIHKQIEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRT 101

Query: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120
           ITAKVHLHC KCE+DLKN LLKHKGIY+VKTD+KAQTLTMEG+IE EKFKSYLK KLQKH
Sbjct: 102 ITAKVHLHCAKCEKDLKNNLLKHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKH 161

Query: 121 VDITVDTKSTDSSK 134
            DIT    + DSSK
Sbjct: 162 ADITT---AVDSSK 172

[Close]

BLAST of Cucsat.G377 vs. TAIR 10
Match: AT1G29000.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-24
Identity = 69/141 (48.94%), Postives = 96/141 (68.09%), Query Frame = 0


Query: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEK----DPPKPIDDKPKPTI 60
           +EKNEI+VKG  ++V+KI KQIEK SKKKVELISPK    +K         + +K    I
Sbjct: 49  LEKNEIKVKG-KIEVVKIHKQIEKWSKKKVELISPKPSEVKKTTTTTTTTSVVEKKTTEI 108

Query: 61  ---VNRIITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYL 120
              V R    KVH+HC +C++DL++KLLKHK I+ VKTD KAQTLT++G+IE+ K  +Y+
Sbjct: 109 KKDVIRTTVLKVHIHCAQCDKDLQHKLLKHKAIHIVKTDTKAQTLTVQGTIESAKLLAYI 168

Query: 121 KNKLQKHVDITVDTKSTDSSK 135
           K K+ KH +I + +K+ +  K
Sbjct: 169 KKKVHKHAEI-ISSKTEEEKK 187

[Close]

BLAST of Cucsat.G377 vs. TAIR 10
Match: AT5G50740.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 2.7e-12
Identity = 38/125 (30.40%), Postives = 73/125 (58.40%), Query Frame = 0


Query: 4   NEIRVKGSNLDVLKIQKQIEKLSKKKVELIS--PKVKPKEKDPPKPIDDKPKPTIVNR-- 63
           +++ VKG   D LK+ +++++ S ++VELIS  P+ KP   +P K   +KPKP       
Sbjct: 64  SKVVVKGEKADPLKVLQRLQRKSHRQVELISPIPEPKPVSDEPEKKEKEKPKPEEKKEEV 123

Query: 64  -IITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQ 123
             +  +VH+HC  C  +++ ++++ KG+ SV+ D KA  ++++G    EK   ++  ++ 
Sbjct: 124 VTVVLRVHMHCEACAMEIQKRIMRMKGVESVEPDFKASQVSVKGVFTPEKLVEFIYKRIG 183

[Close]

BLAST of Cucsat.G377 vs. TAIR 10
Match: AT5G50740.3 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 2.7e-12
Identity = 38/125 (30.40%), Postives = 73/125 (58.40%), Query Frame = 0


Query: 4   NEIRVKGSNLDVLKIQKQIEKLSKKKVELIS--PKVKPKEKDPPKPIDDKPKPTIVNR-- 63
           +++ VKG   D LK+ +++++ S ++VELIS  P+ KP   +P K   +KPKP       
Sbjct: 71  SKVVVKGEKADPLKVLQRLQRKSHRQVELISPIPEPKPVSDEPEKKEKEKPKPEEKKEEV 130

Query: 64  -IITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQ 123
             +  +VH+HC  C  +++ ++++ KG+ SV+ D KA  ++++G    EK   ++  ++ 
Sbjct: 131 VTVVLRVHMHCEACAMEIQKRIMRMKGVESVEPDFKASQVSVKGVFTPEKLVEFIYKRIG 190

[Close]

BLAST of Cucsat.G377 vs. TAIR 10
Match: AT2G28090.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-11
Identity = 44/137 (32.12%), Postives = 76/137 (55.47%), Query Frame = 0


Query: 2   EKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKD-PPKPIDDKPKPTIVNRI 61
           + N++ + G  +D +KI ++++K SKKKVELISPK K   K+   K  +DK +  +    
Sbjct: 64  DSNKLTLIGFIMDPVKIAEKLQKKSKKKVELISPKPKKDTKENNEKKANDKTQTVVAVTT 123

Query: 62  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 121
           +  KV+  C  C + ++  +   KG+Y VK D + +T+T+ G+++ +     LK KL+K 
Sbjct: 124 VVLKVNCSCDGCIKRIQKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKT 183

Query: 122 VDITVDTKSTDSSKSTA 138
           V +  + K     K  A
Sbjct: 184 VQVVPEKKKKKKDKDNA 200

[Close]

BLAST of Cucsat.G377 vs. TAIR 10
Match: AT5G63530.1 (farnesylated protein 3 )

HSP 1 Score: 66.2 bits (160), Expect = 2.3e-11
Identity = 37/126 (29.37%), Postives = 72/126 (57.14%), Query Frame = 0


Query: 5   EIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKP-------KPTIV 64
           ++ VKG   D LK+  ++++ + ++V+L+SP   P      K  +DKP       +P +V
Sbjct: 111 KVVVKGEKADPLKVLARVQRKTHRQVQLLSPIPPPPPPPEKKAEEDKPIVEEKKVEPPVV 170

Query: 65  NRIITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKL 124
             ++  KVH+HC  C  ++K ++++ KG+ S ++D+K+  +T++G  E +K   Y+  + 
Sbjct: 171 VTVV-LKVHMHCEACATEIKKRIMRMKGVESAESDLKSSQVTVKGVFEPQKLVEYVYKRT 230

The following BLAST results are available for this feature:

BLAST of Cucsat.G377 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SHQ8	1.9e-23	48.94	Heavy metal-associated isoprenylated plant protein 4 OS=Arabidopsis thaliana OX=...	[more]
Q9ZUV1	1.9e-10	32.12	Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=...	[more]
Q9C5D3	3.2e-10	29.37	Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=...	[more]
Q9M8T7	2.5e-07	30.08	Heavy metal-associated isoprenylated plant protein 8 OS=Arabidopsis thaliana OX=...	[more]
Q9FJH5	3.3e-07	30.15	Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=...	[more]

BLAST of Cucsat.G377 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134354.1	2.64e-84	100.00	heavy metal-associated isoprenylated plant protein 4 [Cucumis sativus] >KAE86502...	[more]
XP_008438227.1	2.56e-79	96.38	PREDICTED: heavy metal-associated isoprenylated plant protein 3 [Cucumis melo]	[more]
XP_038878052.1	2.61e-71	88.24	heavy metal-associated isoprenylated plant protein 4 [Benincasa hispida]	[more]
XP_022147087.1	1.95e-64	80.60	heavy metal-associated isoprenylated plant protein 4 [Momordica charantia]	[more]
XP_023528992.1	5.49e-63	80.60	heavy metal-associated isoprenylated plant protein 4 [Cucurbita pepo subsp. pepo...	[more]

BLAST of Cucsat.G377 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4G4	3.08e-85	100.00	HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G127060 PE=3 SV...	[more]
A0A1S3AVV8	1.24e-79	96.38	heavy metal-associated isoprenylated plant protein 3 OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1D1E3	9.44e-65	80.60	heavy metal-associated isoprenylated plant protein 4 OS=Momordica charantia OX=3...	[more]
A0A6J1IZ45	5.69e-63	79.85	heavy metal-associated isoprenylated plant protein 4 OS=Cucurbita maxima OX=3661...	[more]
A0A6J1EFL8	1.08e-62	79.85	heavy metal-associated isoprenylated plant protein 4 OS=Cucurbita moschata OX=36...	[more]

BLAST of Cucsat.G377 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G29000.1	1.4e-24	48.94	Heavy metal transport/detoxification superfamily protein	[more]
AT5G50740.1	2.7e-12	30.40	Heavy metal transport/detoxification superfamily protein	[more]
AT5G50740.3	2.7e-12	30.40	Heavy metal transport/detoxification superfamily protein	[more]
AT2G28090.1	1.3e-11	32.12	Heavy metal transport/detoxification superfamily protein	[more]
AT5G63530.1	2.3e-11	29.37	farnesylated protein 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.70.100		coord: 7..73 e-value: 7.4E-12 score: 47.5 coord: 102..166 e-value: 1.0E-8 score: 37.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 180..206
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 170..207
None	No IPR available	PANTHER	PTHR46195:SF12	HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 4	coord: 3..254
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 17..71 e-value: 1.6E-10 score: 41.3 coord: 108..162 e-value: 4.4E-11 score: 43.0
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 10..74 score: 9.480129
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 109..150 e-value: 0.00966431 score: 31.8073
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 18..71 e-value: 3.48558E-7 score: 44.5189
IPR044577	Heavy metal-associated isoprenylated plant protein 4/7/8/17/18/19	PANTHER	PTHR46195	HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 7	coord: 3..254
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 13..71
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 102..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G377.T1	Cucsat.G377.T1	mRNA
Cucsat.G377.T2	Cucsat.G377.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding