Cucsat.G3575 (gene) Cucumber (B10) v3

Overview
NameCucsat.G3575
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionGlucose-methanol-choline (Gmc) oxidoreductase
Locationctg1047: 1186745 .. 1192155 (-)
RNA-Seq ExpressionCucsat.G3575
SyntenyCucsat.G3575
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACATTGAATTCATCATTAAATAAGCAATTATACAGTAGTTACATTGGAAGTCTTTTTCTTTTCTCTGTCATTTTTTTTCATTACCAGATGGATGTAATATATTGGTGTTTGATTTCAACCCATAATTTTCTAGGGGTTTGTTTCTGAGTATAAATCTGTGACCCACACACAAATCAAGACCAACCAGGAGAAGCCACATACAAGTTTGGAAGAAGCAGCTTCCGTTGTTACTGAGACTGCCTTTTTCCTTTTCTGCAAATCAATGGCGTTGGCTGGGACAGTCAAGCTCTTTCTCTTCCTTGTTCTCTTCACTCTCTTACACCTCCTCTCTTCTTGTCAAGGTTGGTTCTTCTGATACCTTCCATTATAATGCAGCTACTTCATACAAAATTCTGTTTCCATTCATTTCTAATAGAAAAAAAAAAAAAACAGAACGACATCTATTGTAGTTGAGGTTCAACTTTCTAGGATTAGAGAGCATCGAATCACTGGTTTCTGAGTTCATCTCATCTTCTTTGCCCTACAACACCCACACACAAGAAATAAACACAGAATTTTGATCTATTTCTCTTCAGCAAAATAACCTGGTTATTCTTCCCATTATTAACAAGCAAAAGAACTCAAAAACTGTGCAAGTGCAGGTAGGGAAAATTGGATAAAATCGCGGTACCCGTTCATCAAGCGCGCAAGCTCGTTCTATCGTGATGGCCATGAAAGAAAGGGTGGTTACGATTACATAATCGTCGGCGGTGGTACCGCCGGTTGTCCATTGGCGGCTACATTGTCACAAAACTTCAGCGTGCTACTACTGGAAAGAGGAGGTGTTCCTTTCACAAATGCGAATGTGTCTTTCCTTGGAAATTTCCATATTGGCCTGGCCGACACTTCCCCGACGTCGGCTTCTCAGGCCTTCGCTTCGACGGATGGAGTTATCAATGCGAGAGCTAGGGTTTTGGGTGGTGGTTCCGCCATTAATGCAGGCTTCTATACACGAGCAAGCACAAGGTTCGTCCACTTCCATTTCATTTTCCCACCATCTTTTAAATTTTTTTTCTTTTAAACCCTCTTTTTTTAATTTTTACCCTAACAATTTTAAATTTAAACTAAAAAACCACAATAAATCTTTAGGTTCATTTGGTTCAAATTAAACTCAAAAGTTGAATTTAATACTTTCTTAGATTTTTTTTACTTTGTTTTTCTCTCTCCCTTAATTATTAAGTTTAAAAAGTGAATTTAGTTAAAAACGTCATCAATGTTGTGAATTAGTTAGGTTAAGTTTAATTTAGTTGAACTTTAAATAAAACCACATTTTGTATTTATTTCTTTTATTCCTAAGATTAAAATTTTAATACAACGTTCCAAACGTTAAGTTAGATACGTAAGAATTTAGCCAATAACTTATATAAGTTATACGAGAACTAAGTAGTTTTGGGTTTAGCTTTTCTACTCCCATTTTGCACTTAAAAGATTATATATCATTTCTTCTTTTTCCATTTTGATCACGATCTCTTAACGAAAGGAAGTTATAAGGAGGTGAATTATCAAAAATATCAAATTTGACAAAATATTTACAACATATAGAAAATTTCATATTATATCAATAATAGACAATGATATGTTTCTATCAGTAACATTGATAGACAAAGATCTACCATTTCTATATAAATTGATATAATCTGAAATTTTACTGTAGCTGACAAATGTTTTAATTTATTTTGCTATTTTTAAAAATGTTCCTCGTAAAAAATCAATATAGATTAGTACATTTGTAAATATTTTTAATCGTTTTTGTTTTTAAAATAATTTTAAAGACAAACTTTAATAAGTTGAGTTTGGTTTATTTTATTATAAATATAAATATTATTTTCTCAAATGATAAGACTACTTGCTTGTTTTATGATTTCTTCCTTTCCCCTTTATATTTGTTTGAAGAGAAGCTTTGGTTTTCCTTTTTCTTATTTTTTTTAAAACAACAAGAAAAATAAATTAGAGTTGGTAAAAAAAGAAAAAGAAAAAACTCAATAATCTAAAGGTAGGTATTAAGGTGACCAATTTGGAGGTGGTCTATTTAAGAACAAAATTCACTTGTTCCTCCCACTAACTCATGTTTAATATCTATCATTATTCTCATTATTATATATTTCAATAATTTATAAAGTCGAGTATCGAACAACATAAGTTTTAAATCGTACTTAGTGATTTTTTCGTCCACTCAATTTAAAAAACGATGGAAATATAAGTATATGTCACTTAAGGAGATTTTGGTCAACTAAGTGGAGTTAATAAGTCATAAGCTAATAAATATGCGTTCGAGGTGTAGAGTTATAAGATAAAATTTTGTGATGAACGTGAAAAGTAAAAACAGAGGAAAATTTGTAGTTATTCGATAATAAATTTATGTTTGGGTCTTGGAGTTAGAATTAGAAGATGAATTTGTTGTGATAAACGTGAGAAAGTAAGAAAAAAAAGAGACAAATGTGTAATTATTCAATAATGGTGAAAAACAATAATGGATAGAAATACGAAAACTTCAAAGGTATTAATAGGAGTGACAATAGTTGGTTGGAGTTGGGGAGTCGGTTTTCGTGCCATCCAACCGATCACGAGTCTATATGGAAATGGCTAAATCGATCTTGACTACTATTTTCAACAAAACCTAGCAATTGAGTTTTTTTTGTTGGTCGATCGATTTTTTTCTCTCCAACTTTTTGGTAGAAAATAAATAATATGACATGACTTTTTAAATGCATCAAATGAATTTAACACTACTAATAAATTATATATATCTATTGATAAACATTGAAAAAAAAGTTGAGTAGATCAAGAATCGTCCTTTCGACCCCGACCGAGCTCTTTCGATTTTTCGACCTAACATATTAAAAATATTAAAAATATGAATGATGGGTAGATAAACACTTCTAATAATATTATGGGTTTTGTAGGTTCATAAAGAGAGTGGGTTGGGACGAGAGGTTGGTGAACGAATCATACTCATGGGTGGAGAACCGTATAGTTCATCGCCCGGAGCTCGCCGATTGGCAGAAGGCTTTCACCGACAGCATGTTGGACGTGGGAATTTCGCCCTTCAACGGCTTTACCTACGATCATCTCTACGGCACCAAAGTCGGAGGGACCATTTTCGACCGCTTCGGCCGCCGTCACACCACAGCCGAGCTTTTAGCTTCCGGGAATCCAGATAAGCTTACGGTTCTGGTTCATGCAACTGTTCAACGACTCATCTTTGACACCACTGACGGTTCGTTTGCTTCTTTCTCTAGCGTCTTTGTGAATTTATTTTTGCCTGTTCATTCCCTGAAGCATTGAGTTCTGCGGATTTCACATTCGTTACTTTGCCTAATTGATTGGGAAATCATGGTGGAATTGTAGAGATTTACATTGCATTTTAGAGATTAATGTGAATTATGAATGTTGGTCGATTTTTGGATGATACAATAAAGTAGGGAAACCGCCAAAAAGTAGCCCGCTTCATAATCTTTTAATAAGACTGACCAGTAAACTGCTGCTGCGAAAAAATGGTGAACTAATGTAGATATGTAAAAGCCTTCTGATTTTTGAAGTGTAATGACTAGATTGGCACAATCTTGAAAGTTCTGACACCTGTTAAGGAACTTTTTGTTCTAATCAAACATTTACAATTCTGAATGTTCAGGTAAGAAACCAAAGGCGATTGGAGTTGTTTTCAAGGATGACATTGGTAATCAGCACGAAGTTTTTCTATCAAGCAATCGGCAGAGTGAAGTTATAATGTCCAGTGGAGCAATCGGAACTCCACAAATGCTCTTGCTCAGTGGCATTGGTCCGAGGGCTGATCTTGAAAAGTGGAACATATCCATGGTGCTTGACAATGAGTTTGTTGGGAAAAACATGGCTGATAATCCCCTGAACGCCATTTTTGTTCCTAGCAACAGACCAGTTAAGCAGTCACTTATACAAGCAGTTGGAATCACGAAGCGTGGTGTTTATATTGAATCTAGCAGTGGATTTGGTCAGTCGGGGGAAAGTATTCACTGCCACCATGGTTTGATGTCGGCTGAGGTAAATTAAACATCACATATTTACTTATAAAAGATAAATAATACGGAGAGAACTTAAGAGAACTGTCTTTAAATTTTAGTGTTCCCAATCTTTGATTTGAGTTATGAAGTTGCTACTTCAAATGAAAACGTTTGAGTTGAACTTGGTTATCTTTGCACACGAGCAAAGTAGATTTGATTTTCAAAATATGTAGAAAGATATAGATTCTGTATCGTACTCAATCATGTGGTTTAACCAGATTGGGCAGCTATCCACCATTCCTCCGAAACAAAGAACGCCTGAAGCAATTCAAGCTTACATCAAAAGTAAACGAGACTTGCCGCATGAGGCATTTAAAGGAGGTTTCGTTCTAGAAAAAATAGCGTATCCCATCTCAAGGGGACAACTGAGCTTGATCAACACTAATGTTGATGACAATCCTGCTGTAACCTTCAACTATTTTGGTCATCCATATGATCTTCACCGTTGTGTTGAAGGGATTCGCATGGTTGCAAAGATCGTGGAGTCTAAATGTTTCACAAATTTCACACAATGTGATGAGGAGACATTAGACAAGCTGCTTAACATTAGTGTAAAGGCCAACATCAACCTCATACCCAAACATACCAATGACACCAAGTCCTTGGAGCAGTTCTGCAAAGACACTGTGATCACAATCTGGCATTACCATGGAGGGTGTCTAGTGGACAAGGTGGTGAGCCATGACCTTAAAGTTCTTGGTGTAACTAGATTGCGCATTGTTGACGGGTCGACACTCAGTGAGTCCCCAGGCACCAATCCTCAAGCAACTGTTATGATGATGGGAAGGTATACTTATGAACATCTTTTTGTGAGCCGTTACTCTCTTCTAAAGAAGAAAGATGAAAAACAGCAACTAACTACAACTGGATGCCCTTTTTTTTTGTGTGTGTGTGTATGCAGGTACATGGGCCTAAAAATCTTGATGGATAGATTGGGAAAGAAAGGTGGAACATAGGAAGCATTAAGACTACAAAGAATTACAAGCAATGTTAGTCGTTAGTGGAAGAGTGACCTGGGTGCTTGATTCTTAAAGAATAGTGGGCATACAGAACTCTGGCTTGGCCTGGAAATTACAAATTTTTTTATATGAGAAGCATTCTTTATATTTAGTCTTTCAGCTACTTGGTTACTATTTGTTTTGTATTATTTTGATAGTTAACGAGTTATTCTTTTAGAGCACTCAAAATAAAAAAAAATAAAAAAAAAAAGAGAGAGAGAGAAAGAGAAGAAGAAAGAGACTGGTCTCAACTAGACTTGTTCTTCATGTTTGAGCTAGACTGTAGTGATCGTGTGTATCTACAGTTATGTATGTATCTTGTGATCTTGATAGAGGACAGAGTGAATAACTTTTGAATGGAATATCTAACTTATTTGCTTTATTCAATTAATTCTTTATCAATTTCTTTTAATTTTATTGGATTAGTGTTAG

Coding sequence (CDS)

ATGGCGTTGGCTGGGACAGTCAAGCTCTTTCTCTTCCTTGTTCTCTTCACTCTCTTACACCTCCTCTCTTCTTGTCAAGGTAGGGAAAATTGGATAAAATCGCGGTACCCGTTCATCAAGCGCGCAAGCTCGTTCTATCGTGATGGCCATGAAAGAAAGGGTGGTTACGATTACATAATCGTCGGCGGTGGTACCGCCGGTTGTCCATTGGCGGCTACATTGTCACAAAACTTCAGCGTGCTACTACTGGAAAGAGGAGGTGTTCCTTTCACAAATGCGAATGTGTCTTTCCTTGGAAATTTCCATATTGGCCTGGCCGACACTTCCCCGACGTCGGCTTCTCAGGCCTTCGCTTCGACGGATGGAGTTATCAATGCGAGAGCTAGGGTTTTGGGTGGTGGTTCCGCCATTAATGCAGGCTTCTATACACGAGCAAGCACAAGGTTCATAAAGAGAGTGGGTTGGGACGAGAGGTTGGTGAACGAATCATACTCATGGGTGGAGAACCGTATAGTTCATCGCCCGGAGCTCGCCGATTGGCAGAAGGCTTTCACCGACAGCATGTTGGACGTGGGAATTTCGCCCTTCAACGGCTTTACCTACGATCATCTCTACGGCACCAAAGTCGGAGGGACCATTTTCGACCGCTTCGGCCGCCGTCACACCACAGCCGAGCTTTTAGCTTCCGGGAATCCAGATAAGCTTACGGTTCTGGTTCATGCAACTGTTCAACGACTCATCTTTGACACCACTGACGGTAAGAAACCAAAGGCGATTGGAGTTGTTTTCAAGGATGACATTGGTAATCAGCACGAAGTTTTTCTATCAAGCAATCGGCAGAGTGAAGTTATAATGTCCAGTGGAGCAATCGGAACTCCACAAATGCTCTTGCTCAGTGGCATTGGTCCGAGGGCTGATCTTGAAAAGTGGAACATATCCATGGTGCTTGACAATGAGTTTGTTGGGAAAAACATGGCTGATAATCCCCTGAACGCCATTTTTGTTCCTAGCAACAGACCAGTTAAGCAGTCACTTATACAAGCAGTTGGAATCACGAAGCGTGGTGTTTATATTGAATCTAGCAGTGGATTTGGTCAGTCGGGGGAAAGTATTCACTGCCACCATGGTTTGATGTCGGCTGAGGTAAATTAA

Protein sequence

MALAGTVKLFLFLVLFTLLHLLSSCQGRENWIKSRYPFIKRASSFYRDGHERKGGYDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPFTNANVSFLGNFHIGLADTSPTSASQAFASTDGVINARARVLGGGSAINAGFYTRASTRFIKRVGWDERLVNESYSWVENRIVHRPELADWQKAFTDSMLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLIFDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADLEKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQSGESIHCHHGLMSAEVN
Homology
BLAST of Cucsat.G3575 vs. ExPASy Swiss-Prot
Match: Q9S746 (Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 1.3e-163
Identity = 271/400 (67.75%), Postives = 330/400 (82.50%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +L+VG+ PFNGFTYDH+ GTK+GGTIFDRFGRRHT AELLA  NP KL VL++ATVQ+++
Sbjct: 195 LLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQKLRVLIYATVQKIV 254

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDT+ G +P+  GV+FKD+ GNQH+  LS+ + SEVI+SSGAIG+PQML+LSGIGP+ +L
Sbjct: 255 FDTS-GTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKEL 314

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           ++  I +VL+NE VGK MADNP+N I VPS  P++QSLIQ VGITK GVY+E+S+GFGQS
Sbjct: 315 QRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQS 374

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
            ESIH H+G+MS +    STIP KQR PEA QAYI   +   HEAF G F+LEK+AYPIS
Sbjct: 375 PESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGSFILEKLAYPIS 434

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RG LSL+NTNVDDNP+VTFNYF HP DL RCVE IR+V+K+V S  F N+TQCD++ + K
Sbjct: 435 RGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTSNRFLNYTQCDKQNVHK 494

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           +L++SVKANINL PK  NDTKS+ QFCKDTV+TIWHYHGGCLV KVVS + KVLGV RLR
Sbjct: 495 MLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLR 554

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGG 401
           ++DGST  ESPGTNPQAT+MMMGRYMG+KIL +RLG K G
Sbjct: 555 VIDGSTFDESPGTNPQATMMMMGRYMGVKILRERLGNKAG 593

BLAST of Cucsat.G3575 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 8.6e-80
Identity = 165/398 (41.46%), Postives = 231/398 (58.04%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +L+VG+ PFNGFT +H  GTK+GG+ FDR GRRH++A+LL       + V V+ATV+R++
Sbjct: 189 LLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVL 248

Query: 61  F---DTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPR 120
                +  G    AIGVV++D +G  H   +    + EVI+S+GA+G+PQ+L LSGIGPR
Sbjct: 249 LASSPSVSGSNVSAIGVVYRDQLGRFHHALIRD--RGEVILSAGALGSPQLLFLSGIGPR 308

Query: 121 ADLEKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGF 180
           + L  W I + LD   VG  + DNP N I +    P++ SLIQ VG+T+ G ++E++S  
Sbjct: 309 SYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNV 368

Query: 181 GQSGESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAY 240
                 +H      S  I          R P A   Y+               ++EKI  
Sbjct: 369 IPFASPLH------SVFI----------RAP-ASPLYVPVTT-----------IMEKILG 428

Query: 241 PISRGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEET 300
           P+S G L L +T+V  NP V FNYF  P DL RCV G R + +I+ S+   +F   +   
Sbjct: 429 PVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFG 488

Query: 301 LDKLLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVT 360
             +   +        +P   ++   +  FC+ TV TIWHYHGG +V KVV  DLKV+GV 
Sbjct: 489 NRRFRFVGAP-----LPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVN 548

Query: 361 RLRIVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRL 396
            LR+VDGST + SPGTNPQAT+MM+GRYMGLK+L +R+
Sbjct: 549 SLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRERM 551

BLAST of Cucsat.G3575 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.6e-65
Identity = 139/394 (35.28%), Postives = 215/394 (54.57%), Query Frame = 0

Query: 2   LDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLIF 61
           L+ G+ P +GF+ DH  GT++ G+ FD  G RH   ELL  GN + L V VHA+V+++IF
Sbjct: 190 LEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIF 249

Query: 62  DTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADLE 121
               G    A GV+++D  G  H  F+ S  + EVI+S+G IGTPQ+LLLSG+GP + L 
Sbjct: 250 SNAPGL--TATGVIYRDSNGTPHRAFVRS--KGEVIVSAGTIGTPQLLLLSGVGPESYLS 309

Query: 122 KWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQSG 181
             NI +VL + +VG+ + DNP N I +    P++ +++  +GI               S 
Sbjct: 310 SLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI---------------SN 369

Query: 182 ESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPISR 241
           +   C    +        T PP    P        +   LP+  F       K+A P+S 
Sbjct: 370 DFYQCSFSSLP------FTTPPFSFFP-------STSYPLPNSTF--AHFASKVAGPLSY 429

Query: 242 GQLSL-INTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 301
           G L+L  ++NV  +P V FNY+ +P DL  CV G++ + +++ +     +   D   ++ 
Sbjct: 430 GSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEG 489

Query: 302 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 361
              + +      +PK   D  + E FC+++V + WHYHGGCLV KV+  D +V G+  LR
Sbjct: 490 FNILGIP-----LPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDALR 544

Query: 362 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDR 395
           +VDGST   +P ++PQ   +M+GRY+G+KIL +R
Sbjct: 550 VVDGSTFPYTPASHPQGFYLMLGRYVGIKILQER 544

BLAST of Cucsat.G3575 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.3e-64
Identity = 139/394 (35.28%), Postives = 215/394 (54.57%), Query Frame = 0

Query: 2   LDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLIF 61
           L+ G+ P +GF+ DH  GT++ G+ FD  G RH   ELL  GN + L V VHA+V+++IF
Sbjct: 190 LEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIF 249

Query: 62  DTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADLE 121
               G    A GV+++D  G  H+ F+ S  + EVI+S+G IGTPQ+LLLSG+GP + L 
Sbjct: 250 SNAPGL--TATGVIYRDSNGTPHQAFVRS--KGEVIVSAGTIGTPQLLLLSGVGPESYLS 309

Query: 122 KWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQSG 181
             NI +VL + +VG+ + DNP N I +    P++ +++  +GI               S 
Sbjct: 310 SLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI---------------SN 369

Query: 182 ESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPISR 241
           +   C    +        T PP    P A          LP+  F       K+A P+S 
Sbjct: 370 DFYQCSFSSLP------FTTPPFGFFPSA-------SYPLPNSTF--AHFASKVAGPLSY 429

Query: 242 GQLSL-INTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 301
           G L+L  ++NV  +P V FNY+ +  DL  CV G++ + +++ +     +   D   ++ 
Sbjct: 430 GSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEG 489

Query: 302 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 361
              + +      +PK   D  + E FC+++V + WHYHGGCLV KV+  D +V G+  LR
Sbjct: 490 FNILGIP-----LPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINALR 544

Query: 362 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDR 395
           +VDGST   +P ++PQ   +M+GRY+G+KIL +R
Sbjct: 550 VVDGSTFPYTPASHPQGFYLMLGRYVGIKILQER 544

BLAST of Cucsat.G3575 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.3e-64
Identity = 141/398 (35.43%), Postives = 219/398 (55.03%), Query Frame = 0

Query: 2   LDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLIF 61
           L+VGI P NGF+ DHL GT++ G+ FD  G RH + ELL  G+P+ L V VHA V+++IF
Sbjct: 191 LEVGILPDNGFSLDHLEGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVHAAVEKIIF 250

Query: 62  DTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADLE 121
            ++D     AIGV++ D  G  H+ F+  +   EVI+S+G IG+PQ+LLLSG+G  + L 
Sbjct: 251 -SSDSSGVTAIGVIYTDSNGTTHQAFVRGD--GEVILSAGPIGSPQLLLLSGVGLESYLT 310

Query: 122 KWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQSG 181
             NIS+V  + +VG+ + DNP N I +    P++ S +  +GIT         S F Q  
Sbjct: 311 SLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGIT---------SDFYQC- 370

Query: 182 ESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPISR 241
                           +S++P    +      +      LP+  F    ++ K+  P+S 
Sbjct: 371 ---------------SISSLP---FSTAPFGFFPNPTYPLPNTTF--AHIVNKVPGPLSH 430

Query: 242 GQLSLINT-NVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 301
           G + L +T +V   P VTFNY+ +  DL  CV G++ + + + S     +   D   ++ 
Sbjct: 431 GTVLLQSTSDVRVAPNVTFNYYSNTTDLAHCVSGMKKIGEFLSSDALKPYKVEDLPGIEG 490

Query: 302 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 361
              + +      +P++  D  + E FC++ V + WHYHGGCLV +V+  D +V G+  LR
Sbjct: 491 FDILGIP-----LPENQTDDAAFETFCREAVASYWHYHGGCLVGEVLDDDFRVTGINALR 550

Query: 362 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKK 399
           +VDGST   +P ++PQ   +M+GRYMG KIL +RL  +
Sbjct: 551 VVDGSTFPSTPASHPQGFYLMLGRYMGTKILQERLASE 550

BLAST of Cucsat.G3575 vs. NCBI nr
Match: XP_004141804.1 (protein HOTHEAD [Cucumis sativus] >KGN45528.1 hypothetical protein Csa_016740 [Cucumis sativus])

HSP 1 Score: 811 bits (2095), Expect = 1.82e-293
Identity = 401/401 (100.00%), Postives = 401/401 (100.00%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI
Sbjct: 188 MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 247

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL
Sbjct: 248 FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 307

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS
Sbjct: 308 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 367

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
           GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS
Sbjct: 368 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 427

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK
Sbjct: 428 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 487

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR
Sbjct: 488 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 547

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT 401
           IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT
Sbjct: 548 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT 588

BLAST of Cucsat.G3575 vs. NCBI nr
Match: KAA0059432.1 (protein HOTHEAD-like [Cucumis melo var. makuwa] >TYK03893.1 protein HOTHEAD-like [Cucumis melo var. makuwa])

HSP 1 Score: 795 bits (2054), Expect = 3.17e-287
Identity = 392/401 (97.76%), Postives = 397/401 (99.00%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +LDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI
Sbjct: 188 LLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 247

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL
Sbjct: 248 FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 307

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           EKWNISMVLDN+FVGK+MADNPLN+IFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS
Sbjct: 308 EKWNISMVLDNDFVGKDMADNPLNSIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 367

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
           GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIA PIS
Sbjct: 368 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIANPIS 427

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMV KIVESKCFTNFTQCD+ETLDK
Sbjct: 428 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVTKIVESKCFTNFTQCDKETLDK 487

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR
Sbjct: 488 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 547

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT 401
           IVDGST SESPGTNPQATVMMMGRYMGLKILMDRLGKK GT
Sbjct: 548 IVDGSTFSESPGTNPQATVMMMGRYMGLKILMDRLGKKAGT 588

BLAST of Cucsat.G3575 vs. NCBI nr
Match: XP_008462353.1 (PREDICTED: protein HOTHEAD-like [Cucumis melo])

HSP 1 Score: 795 bits (2054), Expect = 3.17e-287
Identity = 392/401 (97.76%), Postives = 397/401 (99.00%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +LDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI
Sbjct: 188 LLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 247

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL
Sbjct: 248 FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 307

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           EKWNISMVLDN+FVGK+MADNPLN+IFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS
Sbjct: 308 EKWNISMVLDNDFVGKDMADNPLNSIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 367

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
           GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIA PIS
Sbjct: 368 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIANPIS 427

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMV KIVESKCFTNFTQCD+ETLDK
Sbjct: 428 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVTKIVESKCFTNFTQCDKETLDK 487

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR
Sbjct: 488 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 547

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT 401
           IVDGST SESPGTNPQATVMMMGRYMGLKILMDRLGKK GT
Sbjct: 548 IVDGSTFSESPGTNPQATVMMMGRYMGLKILMDRLGKKAGT 588

BLAST of Cucsat.G3575 vs. NCBI nr
Match: ADN34109.1 (glucose-methanol-choline oxidoreductase [Cucumis melo subsp. melo])

HSP 1 Score: 784 bits (2025), Expect = 2.37e-283
Identity = 389/401 (97.01%), Postives = 394/401 (98.25%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +LDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI
Sbjct: 157 LLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 216

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL
Sbjct: 217 FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 276

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           EKWNISMVLDN+FVGK+MADNPLN+IFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS
Sbjct: 277 EKWNISMVLDNDFVGKDMADNPLNSIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 336

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
           GESIHCHHGLMSAE   LSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIA PIS
Sbjct: 337 GESIHCHHGLMSAE---LSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIANPIS 396

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMV KIVESKCFTNFTQCD+ETLDK
Sbjct: 397 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVTKIVESKCFTNFTQCDKETLDK 456

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR
Sbjct: 457 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 516

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT 401
           IVDGST SESPGTNPQATVMMMGRYMGLKILMDRLGKK GT
Sbjct: 517 IVDGSTFSESPGTNPQATVMMMGRYMGLKILMDRLGKKAGT 554

BLAST of Cucsat.G3575 vs. NCBI nr
Match: XP_038899986.1 (protein HOTHEAD-like [Benincasa hispida])

HSP 1 Score: 773 bits (1996), Expect = 2.56e-278
Identity = 381/401 (95.01%), Postives = 390/401 (97.26%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +LDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHT AELLASGNPDKLTVLVHATVQRLI
Sbjct: 188 LLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTAAELLASGNPDKLTVLVHATVQRLI 247

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDTT+GKKPKAIGVVF DD GNQHEVFLSS+RQSEVI+S+GAIGTPQMLLLSGIGPRADL
Sbjct: 248 FDTTEGKKPKAIGVVFMDDTGNQHEVFLSSSRQSEVILSNGAIGTPQMLLLSGIGPRADL 307

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           EKWNISMVLDNEFVGK+MADNPLN+IFVPSNRPVKQSLIQAVGITK GVYIESSSGFGQS
Sbjct: 308 EKWNISMVLDNEFVGKDMADNPLNSIFVPSNRPVKQSLIQAVGITKLGVYIESSSGFGQS 367

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
           GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQ YIKSKRDLPHEAFKGGFVLEKIA PIS
Sbjct: 368 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQDYIKSKRDLPHEAFKGGFVLEKIANPIS 427

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RG LSLINTNVDDNPAVTFNYFGHPYDLHRCVEG+RM+ KIVESK FTNFTQCDEETLDK
Sbjct: 428 RGHLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGVRMLTKIVESKYFTNFTQCDEETLDK 487

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR
Sbjct: 488 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 547

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT 401
           IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKK G 
Sbjct: 548 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKAGN 588

BLAST of Cucsat.G3575 vs. ExPASy TrEMBL
Match: A0A0A0K7W4 (Glucose-methanol-choline (Gmc) oxidoreductase OS=Cucumis sativus OX=3659 GN=Csa_7G451340 PE=3 SV=1)

HSP 1 Score: 811 bits (2095), Expect = 8.79e-294
Identity = 401/401 (100.00%), Postives = 401/401 (100.00%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI
Sbjct: 188 MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 247

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL
Sbjct: 248 FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 307

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS
Sbjct: 308 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 367

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
           GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS
Sbjct: 368 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 427

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK
Sbjct: 428 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 487

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR
Sbjct: 488 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 547

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT 401
           IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT
Sbjct: 548 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT 588

BLAST of Cucsat.G3575 vs. ExPASy TrEMBL
Match: A0A5A7UU55 (Protein HOTHEAD-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00880 PE=3 SV=1)

HSP 1 Score: 795 bits (2054), Expect = 1.53e-287
Identity = 392/401 (97.76%), Postives = 397/401 (99.00%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +LDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI
Sbjct: 188 LLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 247

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL
Sbjct: 248 FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 307

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           EKWNISMVLDN+FVGK+MADNPLN+IFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS
Sbjct: 308 EKWNISMVLDNDFVGKDMADNPLNSIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 367

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
           GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIA PIS
Sbjct: 368 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIANPIS 427

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMV KIVESKCFTNFTQCD+ETLDK
Sbjct: 428 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVTKIVESKCFTNFTQCDKETLDK 487

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR
Sbjct: 488 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 547

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT 401
           IVDGST SESPGTNPQATVMMMGRYMGLKILMDRLGKK GT
Sbjct: 548 IVDGSTFSESPGTNPQATVMMMGRYMGLKILMDRLGKKAGT 588

BLAST of Cucsat.G3575 vs. ExPASy TrEMBL
Match: A0A1S3CIA9 (protein HOTHEAD-like OS=Cucumis melo OX=3656 GN=LOC103500726 PE=3 SV=1)

HSP 1 Score: 795 bits (2054), Expect = 1.53e-287
Identity = 392/401 (97.76%), Postives = 397/401 (99.00%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +LDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI
Sbjct: 188 LLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 247

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL
Sbjct: 248 FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 307

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           EKWNISMVLDN+FVGK+MADNPLN+IFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS
Sbjct: 308 EKWNISMVLDNDFVGKDMADNPLNSIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 367

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
           GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIA PIS
Sbjct: 368 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIANPIS 427

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMV KIVESKCFTNFTQCD+ETLDK
Sbjct: 428 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVTKIVESKCFTNFTQCDKETLDK 487

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR
Sbjct: 488 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 547

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT 401
           IVDGST SESPGTNPQATVMMMGRYMGLKILMDRLGKK GT
Sbjct: 548 IVDGSTFSESPGTNPQATVMMMGRYMGLKILMDRLGKKAGT 588

BLAST of Cucsat.G3575 vs. ExPASy TrEMBL
Match: E5GCB0 (Glucose-methanol-choline oxidoreductase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 784 bits (2025), Expect = 1.15e-283
Identity = 389/401 (97.01%), Postives = 394/401 (98.25%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +LDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI
Sbjct: 157 LLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 216

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL
Sbjct: 217 FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 276

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           EKWNISMVLDN+FVGK+MADNPLN+IFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS
Sbjct: 277 EKWNISMVLDNDFVGKDMADNPLNSIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 336

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
           GESIHCHHGLMSAE   LSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIA PIS
Sbjct: 337 GESIHCHHGLMSAE---LSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIANPIS 396

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMV KIVESKCFTNFTQCD+ETLDK
Sbjct: 397 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVTKIVESKCFTNFTQCDKETLDK 456

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR
Sbjct: 457 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 516

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGGT 401
           IVDGST SESPGTNPQATVMMMGRYMGLKILMDRLGKK GT
Sbjct: 517 IVDGSTFSESPGTNPQATVMMMGRYMGLKILMDRLGKKAGT 554

BLAST of Cucsat.G3575 vs. ExPASy TrEMBL
Match: A0A6J1HK27 (protein HOTHEAD-like OS=Cucurbita moschata OX=3662 GN=LOC111464276 PE=3 SV=1)

HSP 1 Score: 748 bits (1931), Expect = 7.47e-269
Identity = 367/400 (91.75%), Postives = 386/400 (96.50%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +LDVGISPFNGFTYDHLYGTKVGGTIFD  GRRHTTAELLASGNPDKLTVLVHATVQRLI
Sbjct: 183 LLDVGISPFNGFTYDHLYGTKVGGTIFDSSGRRHTTAELLASGNPDKLTVLVHATVQRLI 242

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDTTDGKKPKAIGVVFKD+ GN+H+V LSSN +SEVI+SSGAIGTPQMLLLSGIGPR DL
Sbjct: 243 FDTTDGKKPKAIGVVFKDETGNEHKVLLSSNSESEVILSSGAIGTPQMLLLSGIGPRPDL 302

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           EKWNISMVLDNEFVGK+MADNP+NAIFVPSNRPV+QSLIQAVGITK GVYIESSSGFGQS
Sbjct: 303 EKWNISMVLDNEFVGKDMADNPMNAIFVPSNRPVQQSLIQAVGITKLGVYIESSSGFGQS 362

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
            ESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIK+KRDLPHEAFKGGFVL KIA PIS
Sbjct: 363 DESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKTKRDLPHEAFKGGFVLGKIANPIS 422

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           +GQLSLINTNVDDNPAVTFNYF HPYDLHRCVEG+RM+ KIV+SK FTNFTQCDEETL++
Sbjct: 423 KGQLSLINTNVDDNPAVTFNYFHHPYDLHRCVEGVRMLTKIVDSKYFTNFTQCDEETLER 482

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           LLNISVKANINLIPKH+NDTKSLEQFCKD+VITIWHYHGGCLVDKVVSHDLKVLGVTRLR
Sbjct: 483 LLNISVKANINLIPKHSNDTKSLEQFCKDSVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 542

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGG 400
           IVDGSTLS+SPGTNPQATVMMMGRYMGLKILMDRLGKK G
Sbjct: 543 IVDGSTLSKSPGTNPQATVMMMGRYMGLKILMDRLGKKSG 582

BLAST of Cucsat.G3575 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 577.4 bits (1487), Expect = 8.9e-165
Identity = 271/400 (67.75%), Postives = 330/400 (82.50%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +L+VG+ PFNGFTYDH+ GTK+GGTIFDRFGRRHT AELLA  NP KL VL++ATVQ+++
Sbjct: 195 LLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQKLRVLIYATVQKIV 254

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDT+ G +P+  GV+FKD+ GNQH+  LS+ + SEVI+SSGAIG+PQML+LSGIGP+ +L
Sbjct: 255 FDTS-GTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKEL 314

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           ++  I +VL+NE VGK MADNP+N I VPS  P++QSLIQ VGITK GVY+E+S+GFGQS
Sbjct: 315 QRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQS 374

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
            ESIH H+G+MS +    STIP KQR PEA QAYI   +   HEAF G F+LEK+AYPIS
Sbjct: 375 PESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGSFILEKLAYPIS 434

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RG LSL+NTNVDDNP+VTFNYF HP DL RCVE IR+V+K+V S  F N+TQCD++ + K
Sbjct: 435 RGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTSNRFLNYTQCDKQNVHK 494

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           +L++SVKANINL PK  NDTKS+ QFCKDTV+TIWHYHGGCLV KVVS + KVLGV RLR
Sbjct: 495 MLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLR 554

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGG 401
           ++DGST  ESPGTNPQAT+MMMGRYMG+KIL +RLG K G
Sbjct: 555 VIDGSTFDESPGTNPQATMMMMGRYMGVKILRERLGNKAG 593

BLAST of Cucsat.G3575 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 528.5 bits (1360), Expect = 4.7e-150
Identity = 255/400 (63.75%), Postives = 310/400 (77.50%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +L+VG+ PFNGFTYDH+ GTK+GGTIFDRFGRRHT AELLA  NP KL VL++ATVQ+++
Sbjct: 195 LLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQKLRVLIYATVQKIV 254

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           FDT+ G +P+  GV+FKD+ GNQH+  LS+ + SEVI+SSGAIG+PQML+LSGIGP+ +L
Sbjct: 255 FDTS-GTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKEL 314

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQS 180
           ++  I +VL+NE VGK MADNP+N I VPS  P++QSLIQ VGITK GVY+E+S+GFGQS
Sbjct: 315 QRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQS 374

Query: 181 GESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIAYPIS 240
            ESIH H+G+MS +    STIP KQR PEA QAYI   +   HEAF G F+LEK+AYPIS
Sbjct: 375 PESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGSFILEKLAYPIS 434

Query: 241 RGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEETLDK 300
           RG LSL+NTNVDDNP+VTFNYF HP                           CD++ + K
Sbjct: 435 RGHLSLVNTNVDDNPSVTFNYFKHP---------------------------CDKQNVHK 494

Query: 301 LLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLR 360
           +L++SVKANINL PK  NDTKS+ QFCKDTV+TIWHYHGGCLV KVVS + KVLGV RLR
Sbjct: 495 MLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLR 554

Query: 361 IVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKKGG 401
           ++DGST  ESPGTNPQAT+MMMGRYMG+KIL +RLG K G
Sbjct: 555 VIDGSTFDESPGTNPQATMMMMGRYMGVKILRERLGNKAG 566

BLAST of Cucsat.G3575 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 382.1 bits (980), Expect = 5.5e-106
Identity = 195/400 (48.75%), Postives = 260/400 (65.00%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +L+ G  P+NGFTYDH+YGTK+GGTIFDR G RHT A+LL   NP  + V +HA+V +++
Sbjct: 178 LLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKIL 237

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           F T    +PKA GV+F+D  G  H+  L  N  +EVI+S+GAIG+PQ+L+LSGIGP A L
Sbjct: 238 FTTKGRPRPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHL 297

Query: 121 EKWNIS-MVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSG--F 180
               I  +VLD+  VG+ M DNP+NAIF+PS  PV+ SLIQ VGITK   YIE +SG  F
Sbjct: 298 AAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIF 357

Query: 181 GQSGESIHCH---HGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEK 240
             S          + L   +  + ++      + ++I  +      L +   + G +L+K
Sbjct: 358 SYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQK 417

Query: 241 IAYPISRGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCD 300
           IA PISRG L L NTN DDNP+V FNY+  P DL  CVEGI  + K++ SK F+ F   D
Sbjct: 418 IAGPISRGHLELRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPD 477

Query: 301 EETLDKLLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVL 360
             T+  LL++ +    NL P+H     +L QFC DTV+TIWHYHGGC V +VV  + +VL
Sbjct: 478 -ATIHGLLDLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVL 537

Query: 361 GVTRLRIVDGSTLSESPGTNPQATVMMMGRYMGLKILMDR 395
           G+  LR++DGST  +SPGTNPQATVMM+GRYMG +IL +R
Sbjct: 538 GIDSLRVIDGSTFLKSPGTNPQATVMMLGRYMGQRILQER 576

BLAST of Cucsat.G3575 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 382.1 bits (980), Expect = 5.5e-106
Identity = 195/400 (48.75%), Postives = 260/400 (65.00%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +L+ G  P+NGFTYDH+YGTK+GGTIFDR G RHT A+LL   NP  + V +HA+V +++
Sbjct: 145 LLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKIL 204

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           F T    +PKA GV+F+D  G  H+  L  N  +EVI+S+GAIG+PQ+L+LSGIGP A L
Sbjct: 205 FTTKGRPRPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHL 264

Query: 121 EKWNIS-MVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSG--F 180
               I  +VLD+  VG+ M DNP+NAIF+PS  PV+ SLIQ VGITK   YIE +SG  F
Sbjct: 265 AAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIF 324

Query: 181 GQSGESIHCH---HGLMSAEIGQLSTIPPKQRTPEAIQAYIKSKRDLPHEAFKGGFVLEK 240
             S          + L   +  + ++      + ++I  +      L +   + G +L+K
Sbjct: 325 SYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQK 384

Query: 241 IAYPISRGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCD 300
           IA PISRG L L NTN DDNP+V FNY+  P DL  CVEGI  + K++ SK F+ F   D
Sbjct: 385 IAGPISRGHLELRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPD 444

Query: 301 EETLDKLLNISVKANINLIPKHTNDTKSLEQFCKDTVITIWHYHGGCLVDKVVSHDLKVL 360
             T+  LL++ +    NL P+H     +L QFC DTV+TIWHYHGGC V +VV  + +VL
Sbjct: 445 -ATIHGLLDLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVL 504

Query: 361 GVTRLRIVDGSTLSESPGTNPQATVMMMGRYMGLKILMDR 395
           G+  LR++DGST  +SPGTNPQATVMM+GRYMG +IL +R
Sbjct: 505 GIDSLRVIDGSTFLKSPGTNPQATVMMLGRYMGQRILQER 543

BLAST of Cucsat.G3575 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 381.3 bits (978), Expect = 9.4e-106
Identity = 198/406 (48.77%), Postives = 261/406 (64.29%), Query Frame = 0

Query: 1   MLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLI 60
           +L+ GI P NGFTYDH+ GTK GGTIFDR G RHT A+LL   +P  +TVL+HATV R++
Sbjct: 178 LLEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRIL 237

Query: 61  FDTTDGKKPKAIGVVFKDDIGNQHEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADL 120
           F T    KP A GVV++D  G  H  +L     SE+I+S+G +G+PQ+L+LSG+GP A L
Sbjct: 238 FRTRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQL 297

Query: 121 EKWNISMVLDNEFVGKNMADNPLNAIFVPSNRPVKQSLIQAVGITKRGVYIESSSG--FG 180
           +  NI++V+D   VG+ M DNP+NA+FVPS  PV+ SLI+ VGIT  G Y+E++ G  FG
Sbjct: 298 QAQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFG 357

Query: 181 QSGESIHCHHGLMSAEIGQLSTIP-PKQRTPEAIQAYIKSKRDLPH-EAFKGGFVLEKIA 240
             G              G  ST       +P A      S   L   + F+GGF+LEK+ 
Sbjct: 358 GGG----------GGSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVM 417

Query: 241 YPISRGQLSLINTNVDDNPAVTFNYFGHPYDLHRCVEGIRMVAKIVESKCFTNFTQCDEE 300
            P+S G L L   N  DNP VTFNYF HP DL RCV GI+ + ++V+SK F+ +   D  
Sbjct: 418 GPLSTGHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYAD-V 477

Query: 301 TLDKLLNISVKANINLIPKHTNDTKSL----EQFCKDTVITIWHYHGGCLVDKVVSHDLK 360
           + + LLN++    +NL P  +    SL    E+FC+ TV TIWHYHGGC+V +VV  D K
Sbjct: 478 SFEYLLNLTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYK 537

Query: 361 VLGVTRLRIVDGSTLSESPGTNPQATVMMMGRYMGLKILMDRLGKK 399
           V+G+ RLR++D ST+   PGTNPQATVMM+GRYMG+KIL +RL KK
Sbjct: 538 VIGIDRLRVIDMSTVGYCPGTNPQATVMMLGRYMGVKILRERLTKK 572

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S7461.3e-16367.75Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1[more]
Q9SSM28.6e-8041.46(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
P527061.6e-6535.28(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K21.3e-6435.28(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
O500481.3e-6435.43(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004141804.11.82e-293100.00protein HOTHEAD [Cucumis sativus] >KGN45528.1 hypothetical protein Csa_016740 [C... [more]
KAA0059432.13.17e-28797.76protein HOTHEAD-like [Cucumis melo var. makuwa] >TYK03893.1 protein HOTHEAD-like... [more]
XP_008462353.13.17e-28797.76PREDICTED: protein HOTHEAD-like [Cucumis melo][more]
ADN34109.12.37e-28397.01glucose-methanol-choline oxidoreductase [Cucumis melo subsp. melo][more]
XP_038899986.12.56e-27895.01protein HOTHEAD-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0K7W48.79e-294100.00Glucose-methanol-choline (Gmc) oxidoreductase OS=Cucumis sativus OX=3659 GN=Csa_... [more]
A0A5A7UU551.53e-28797.76Protein HOTHEAD-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347... [more]
A0A1S3CIA91.53e-28797.76protein HOTHEAD-like OS=Cucumis melo OX=3656 GN=LOC103500726 PE=3 SV=1[more]
E5GCB01.15e-28397.01Glucose-methanol-choline oxidoreductase OS=Cucumis melo subsp. melo OX=412675 PE... [more]
A0A6J1HK277.47e-26991.75protein HOTHEAD-like OS=Cucurbita moschata OX=3662 GN=LOC111464276 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G72970.18.9e-16567.75Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.24.7e-15063.75Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.15.5e-10648.75Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.25.5e-10648.75Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.19.4e-10648.77Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 41..361
e-value: 8.2E-77
score: 260.4
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 55..351
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 56..165
e-value: 1.1E-10
score: 41.4
coord: 216..329
e-value: 1.6E-18
score: 67.1
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 288..302
IPR031206Protein Hothead-likePANTHERPTHR45968:SF5PROTEIN HOTHEADcoord: 7..382
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 7..382

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G3575.T2Cucsat.G3575.T2mRNA
Cucsat.G3575.T5Cucsat.G3575.T5mRNA
Cucsat.G3575.T4Cucsat.G3575.T4mRNA
Cucsat.G3575.T1Cucsat.G3575.T1mRNA
Cucsat.G3575.T3Cucsat.G3575.T3mRNA
Cucsat.G3575.T6Cucsat.G3575.T6mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors