Cucsat.G3354 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G3354
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Stachyose synthase
Location	ctg1041: 5849349 .. 5853297 (+)
RNA-Seq Expression	Cucsat.G3354
Synteny	Cucsat.G3354

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAAAAGCTACTAAGATTATCCATTAAATTCGTAATTTTAGGGATTTGTGGGTCCAATAGCAAATTAAAAGAAATGTATATGTGCATAAAACAAAATTAAAGCGAAATCACCGACTAACATAACTAACTTAACCAACCCCTTATCTAATTTAATTAAAGCTCTTTATGTCTTTTGGTCAACATGACACAATTGGACTCTATTCTACTTTTCAAAAAATAAATTCTTCCCACGAATTCCAGAACATCCGTGTATATGCAAGTGTCATAGTTATATTTGCTTGATGAACCTCAACCAAAATTCTTCAGAGCCTTTTACTTTTATATCATCAAACCTTATTCCTTGTTGGTTTCAGCTTTTGGATTTGAATAAATTCCAGTGTCCACTCAACACAATTTGTTGATTGTTTTCTCATGGTGTGTGTCATCATTTTCAGGTGGTAAATTTGGCTTCTAATTGATCAAGATAAGGTCTTTTCACTTTTTACCTATTTATACCCTTCAGTCATAAACAAACTACTACTTAAATTGATTCTCTGTCTTCAATGAGGTGAAGTTTTCTCTTTTTCCTTCCCACTTGAACTTTTCCTCCTTTAATTTTGGATAACTTCTTTGATTCTACTTTGTGCAACTGTTTGTTTGCATTCACCTATTTCATTCTGATGGTTTGCATGGTTTCTCTATAAAACCCATCAAAGGTTTCAGCTCTTTCTGCATACCATTCTTCTCTTTCCTTCTAGCTACACTAGATTACAAATACCAATTACCAACCTTCTTTTGTTCTTCTCATTACAAGTGTAGTTTTTTGTTTGAGCTTACCACCTGGCTTTGATATTGTCCCTGTATGGTCACAGGGTTGTTTGTCTTTCATTGAAATTTGTTAGAAAGCTTCCAAATTTCTGAGTGACTAGAGATTTATTTCTCCTTGTTTATCTTATTTCCAAGTTCTTTGTCAACCATCTGCCATGGCACCTCCAAATGACCCAGCTGCCTTAAACGCTTCTGTTCTGAAATCTGATGGTTTGGAAAATCTTATTGATTTTTCAGATGGGAAGATCAGTGTTAAAGGAGTTCCAGTGCTGTCCGAAGTCCCAACCAATGTCTTTTTCAGTCCTTTCTCTTCGATAAGCCAATCCTCTGATGCACCACTTCCTTTGCTCCAACGTGTGCATAGTCTGTCCTATAAGGGCGGATTTCTTGGTTTCGACCAAACACAGCCTTCTGATAGGCTGACGAATTCTTTGGGAAAATTCAAGGGTAGGGAGTTTGTGAGTGTTTTCAGGTTCAAAACATGGTGGTCGACCATGTGGGTTGGGAATTCTGGGTCGGATTTACAAATGGAAACTCAATGGGTCATGCTAAATATCCCTGAAATAAAGTCATATGTCGTTATCATTCCCATTATTGAAGGAAGTTTCAGGTCTGCCATGCATCCTGGGACTGATGGGCAAGTCTTGATTTGTGCTGAAAGTGGCTCAACTCATGTGAAAACATCAAGCTTTGATGCCATAGCCTACGTTCATGTGTCTGATAACCCCTACAGGTTAATGAAAGAGGCCTATGCTGCTGTTAGAGTCCATTTGAATACTTTTAGACTCTTGGAAGAGAAGCCTGTCACACATCTGGTGGACAAATTCGGTTGGTGCACCTGGGATGCTTTTTACTTAACAGTAGACCCTGTTGGAATTTGGAATGGGGTTAGTGATTTTGTTGAAGGTGGTATCTCGCCAAGGTTTCTCATCATTGATGATGGATGGCAAAGTATCAATTTGGATGGTGAAGACCCGACTCGAGATGCAAAAAATCTTGTTCTGGGTGGAACTCAAATGACTGCTAGGCTCTATAGATTTGATGAGTGTGAAAAGTTCAGAAAGTACAAAGGTGGATCTTTGACGGGTCCAAATGCTCCGTCGTTTGATCCAAAAAAGCCAAAGTTGTTGATCGCGAAGGCAATCGAGATTGAGCATGCTGAGAAAGAAAGAGACAAGGCCATTGGCTCAGGAGTCACTAATGTCTCTAAGTTTGAGACCAAAATTCAGAAGCTAAAGGAAGAGTTGCATGGGATTTTTGGGAAAGAAGAAGAAGAAGAAAGTAGTGCCATAAACAAAGGTTGTACAAGCTGTTCTTGCAAGGCAGATAACTCTGGAATGAAGGCCTTCACTAGGGACTTAAGAACAAAATTCAAAGGTTTGGACGATATCTTCGTTTGGCATGCTCTGGCTGGTGCTTGGGGTGGTGTAAGGCCTGGTGCTACCCATCTGAATTCCAAGATAGTTCCCTGCAAGCTCTCTCCTGGGCTCGATGGCACAATGACTGATCTTGCTGTTGTGAAGATCATCGAAGGAAGCATTGGACTCGTTCATCCTGATCAAGCTGACGACTTCTTCGATTCCATGCATTCTTATCTTTCTAAAGTTGGGATTACAGGAGTGAAAGTTGATGTGATGCACGTAAGTATCTTGAAAACAAAGCCTTTCAGTTTAATTTTTTAAACTTAGTGTGATTAATAACTGATCTATCAACTTTTTCTTCTTAATGTTTGTTCAATTCAGACTCTAGAATATGTCTCAGAAGAATATGGAGGAAGAGTTGATCTTGCAAAAGCCTATTATAAGGGTCTGACCAACTCTCTTCTTAAGAACTTCAAAGGGACTGGCCTTTTCTCTAGTATGCAACAATGCAATGATTTCTTCTACCTTGGCACAAAGCAAAACTCCATAGGAAGAGTTGGTAAGAATTTCAACTTTTTCCATTTCTTCGTTCTTCAGTCGTTTGAGTTATAAAAAAATTGACTACAACCAATATTTTGGGGTACAGGCGATGATTTTTGGTTTCAAGATCCAAATGGTGATCCCATGGGTGTTTACTGGTTACAAGGTGTCCATATGATCCACTGTGCATACAACAGCATGTGGATGGGGCAAATCATACAGCCTGATTGGGACATGTTCCAATCAGACCATCTATGTGCAAAATTCCATGCAGGATCAAGAGCCATCTGTGGAGGTCCTGTGTATGTGAGTGATTCAGTGGGCGGCCACAATTTTGATCTCATAAAGCAGCTCGTGTATCCTGATGGAACTATTCCTAGATGCCAACATTTTGCCCTCCCCACTAGAGACTGCCTCTTCAAGAACCCACTATTTGACAACAAAACTGTTCTAAAGATCTGGAACCTTAACAAGGTACTACTTCCAACTTCTATTTTTTACTTACTAAAACATCCGTTTCTTCATATCTTTCCAAAACATGACATATCATTTCATCTTCTTGGTTAAAATTTAATTGCAGTATGGAGGTGTAATTGGGACTTTCAACTGCCAAGGAGCAGGGTGGGACCCAAAAGAACAAAGAATCAAGGGACATCCCGAATGCTACAAACCAATGTCCACAACAGTGCACGTCAATGATATAGAATGGGACCAAAAACCAGAAGCAGCCCCAATGGGGAATTTCGTCGAATACATTGTGTACCTGAACCAAGCTGAACAAATTCTCCACACGACCCCAAAATCCGAACCGCTAAAAGCGACCATTCAACCATCTACATTCGAACTCTTCAACTTCATACCGCTAAGAAAGCTAGGTTCCAACATCAAATTCGCTCCCATTGGCCTGACGAACATGTTCAACTGTTCAGGAACCATTCAGCATTTGAAGTATAACGAAAATGGAGTAGAATTGAAAGTGAAAGGAGGAGGAAATTTCTTGGCTTACTCAAGTGGGTCACCGAAGAAATGCGTCTCCAATGGAATTGAAGTGGAATTTGAATGGAAATCCGATGGAAAGCTGAGTTTTGATCTTCATTGGATTGAAGAAGCTGGTGGAGTTTCTAATTTGGATATTTTCTTTTGAGAGAGAGAGAGAGAGAGAGAGAGAAAAAAATGAATATCTAAGTTGAAATTTTATTTAATTTCATGCATTTTGTTGTGTTGTGTTAAGTGACATGTGTGCTAAGGAATAGCCATGTCAATTAAATAAAAAGCTTTTATCATTATTATTATTACTATTTTTGTATTTTTCAACTTTTTATTT

Coding sequence (CDS)

ATGGCACCTCCAAATGACCCAGCTGCCTTAAACGCTTCTGTTCTGAAATCTGATGGTTTGGAAAATCTTATTGATTTTTCAGATGGGAAGATCAGTGTTAAAGGAGTTCCAGTGCTGTCCGAAGTCCCAACCAATGTCTTTTTCAGTCCTTTCTCTTCGATAAGCCAATCCTCTGATGCACCACTTCCTTTGCTCCAACGTGTGCATAGTCTGTCCTATAAGGGCGGATTTCTTGGTTTCGACCAAACACAGCCTTCTGATAGGCTGACGAATTCTTTGGGAAAATTCAAGGGTAGGGAGTTTGTGAGTGTTTTCAGGTTCAAAACATGGTGGTCGACCATGTGGGTTGGGAATTCTGGGTCGGATTTACAAATGGAAACTCAATGGGTCATGCTAAATATCCCTGAAATAAAGTCATATGTCGTTATCATTCCCATTATTGAAGGAAGTTTCAGGTCTGCCATGCATCCTGGGACTGATGGGCAAGTCTTGATTTGTGCTGAAAGTGGCTCAACTCATGTGAAAACATCAAGCTTTGATGCCATAGCCTACGTTCATGTGTCTGATAACCCCTACAGGTTAATGAAAGAGGCCTATGCTGCTGTTAGAGTCCATTTGAATACTTTTAGACTCTTGGAAGAGAAGCCTGTCACACATCTGGTGGACAAATTCGGTTGGTGCACCTGGGATGCTTTTTACTTAACAGTAGACCCTGTTGGAATTTGGAATGGGGTTAGTGATTTTGTTGAAGGTGGTATCTCGCCAAGGTTTCTCATCATTGATGATGGATGGCAAAGTATCAATTTGGATGGTGAAGACCCGACTCGAGATGCAAAAAATCTTGTTCTGGGTGGAACTCAAATGACTGCTAGGCTCTATAGATTTGATGAGTGTGAAAAGTTCAGAAAGTACAAAGGTGGATCTTTGACGGGTCCAAATGCTCCGTCGTTTGATCCAAAAAAGCCAAAGTTGTTGATCGCGAAGGCAATCGAGATTGAGCATGCTGAGAAAGAAAGAGACAAGGCCATTGGCTCAGGAGTCACTAATGTCTCTAAGTTTGAGACCAAAATTCAGAAGCTAAAGGAAGAGTTGCATGGGATTTTTGGGAAAGAAGAAGAAGAAGAAAGTAGTGCCATAAACAAAGGTTGTACAAGCTGTTCTTGCAAGGCAGATAACTCTGGAATGAAGGCCTTCACTAGGGACTTAAGAACAAAATTCAAAGGTTTGGACGATATCTTCGTTTGGCATGCTCTGGCTGGTGCTTGGGGTGGTGTAAGGCCTGGTGCTACCCATCTGAATTCCAAGATAGTTCCCTGCAAGCTCTCTCCTGGGCTCGATGGCACAATGACTGATCTTGCTGTTGTGAAGATCATCGAAGGAAGCATTGGACTCGTTCATCCTGATCAAGCTGACGACTTCTTCGATTCCATGCATTCTTATCTTTCTAAAGTTGGGATTACAGGAGTGAAAGTTGATGTGATGCACGTAAGTATCTTGAAAACAAAGCCTTTCAGTTTAATTTTTTAA

Protein sequence

MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFDAIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVGIWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKLKEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMHVSILKTKPFSLIF

Homology

[Close]

BLAST of Cucsat.G3354 vs. ExPASy Swiss-Prot
Match: Q93XK2 (Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 1.6e-176
Identity = 300/497 (60.36%), Postives = 377/497 (75.86%), Query Frame = 0


Query: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQ--SS 60
           MAPP     LN++       E++ D S+ K  VKG P+  +VP NV F  FSSI +   S
Sbjct: 1   MAPP-----LNSTTSNLIKTESIFDLSERKFKVKGFPLFHDVPENVSFRSFSSICKPSES 60

Query: 61  DAPLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGN 120
           +AP  LLQ+V + S+KGGF GF    PSDRL NS+G F G++F+S+FRFKTWWST W+G 
Sbjct: 61  NAPPSLLQKVLAYSHKGGFFGFSHETPSDRLMNSIGSFNGKDFLSIFRFKTWWSTQWIGK 120

Query: 121 SGSDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSS 180
           SGSDLQMETQW+++ +PE KSYVVIIPIIE  FRSA+ PG +  V I AESGST VK S+
Sbjct: 121 SGSDLQMETQWILIEVPETKSYVVIIPIIEKCFRSALFPGFNDHVKIIAESGSTKVKEST 180

Query: 181 FDAIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDP 240
           F++IAYVH S+NPY LMKEAY+A+RVHLN+FRLLEEK + +LVDKFGWCTWDAFYLTV+P
Sbjct: 181 FNSIAYVHFSENPYDLMKEAYSAIRVHLNSFRLLEEKTIPNLVDKFGWCTWDAFYLTVNP 240

Query: 241 VGIWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDEC 300
           +GI++G+ DF +GG+ PRF+IIDDGWQSI+ DG DP  DAKNLVLGG QM+ RL+RFDEC
Sbjct: 241 IGIFHGLDDFSKGGVEPRFVIIDDGWQSISFDGYDPNEDAKNLVLGGEQMSGRLHRFDEC 300

Query: 301 EKFRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQ 360
            KFRKY+ G L GPN+P +DP     LI K IE E   K+R++AI S  +++++ E+KI+
Sbjct: 301 YKFRKYESGLLLGPNSPPYDPNNFTDLILKGIEHEKLRKKREEAISSKSSDLAEIESKIK 360

Query: 361 KLKEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHAL 420
           K+ +E+  +FG E+              S      G+KAFT+DLRTKFKGLDD++VWHAL
Sbjct: 361 KVVKEIDDLFGGEQFSSGEK--------SEMKSEYGLKAFTKDLRTKFKGLDDVYVWHAL 420

Query: 421 AGAWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMH 480
            GAWGGVRP  THL++KIVPCKLSPGLDGTM DLAVV+I + S+GLVHP QA++ +DSMH
Sbjct: 421 CGAWGGVRPETTHLDTKIVPCKLSPGLDGTMEDLAVVEISKASLGLVHPSQANELYDSMH 480

Query: 481 SYLSKVGITGVKVDVMH 496
           SYL++ GITGVKVDV+H
Sbjct: 481 SYLAESGITGVKVDVIH 484

[Close]

BLAST of Cucsat.G3354 vs. ExPASy Swiss-Prot
Match: Q9SYJ4 (Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=RFS4 PE=2 SV=3)

HSP 1 Score: 575.9 bits (1483), Expect = 4.6e-163
Identity = 283/475 (59.58%), Postives = 352/475 (74.11%), Query Frame = 0


Query: 22  NLIDFSDGKISVK-GVPVLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGF 81
           N  + S+G +  K   P+L +VP NV F+PFSS S S+DAPLP+L RV + ++KGGFLGF
Sbjct: 32  NSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGF 91

Query: 82  DQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVMLNIPEIKSY 141
            +  PSDRLTNSLG+F+ REF+S+FRFK WWST W+G SGSDLQ ETQWVML IPEI SY
Sbjct: 92  TKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVMLKIPEIDSY 151

Query: 142 VVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFDAIAYVHVSDNPYRLMKEAYA 201
           V IIP IEG+FR+++ PG  G VLICAESGST VK SSF +IAY+H+ DNPY LMKEA++
Sbjct: 152 VAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEAFS 211

Query: 202 AVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVGIWNGVSDFVEGGISPRFLII 261
           A+RVH+NTF+LLEEK +  +VDKFGWCTWDA YLTVDP  IW GV +F +GG+ P+F+II
Sbjct: 212 ALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFVII 271

Query: 262 DDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLTGPNAPSFDPK 321
           DDGWQSIN DG++  +DA+NLVLGG QMTARL  F EC+KFR YKGGS    +A  F+P 
Sbjct: 272 DDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPL 331

Query: 322 KPKLLIAKAIEIEHAEKERDKAI-GSGVTNVSKFETKIQKLKEELHGIFGKEEEEESSAI 381
           KPK+LI KA E   A   R K +  SG  ++++ + KI+ L EEL+ +F + E+EES   
Sbjct: 332 KPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLG- 391

Query: 382 NKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGA-THLNSKIVP 441
                  S     SGM AFT+DLR +FK LDDI+VWHAL GAW GVRP     L +K+ P
Sbjct: 392 -------SDDVSGSGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKVAP 451

Query: 442 CKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDV 494
            +LSP L  TM DLAV K++E  IGLVHP +A +F+DSMHSYL+ VG+TG K+DV
Sbjct: 452 FELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDV 498

[Close]

BLAST of Cucsat.G3354 vs. ExPASy Swiss-Prot
Match: Q8VWN6 (Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 5.6e-92
Identity = 184/483 (38.10%), Postives = 257/483 (53.21%), Query Frame = 0


Query: 33  VKGVPVLSEVPTNVFF------SPFSSISQSSDAPLPLLQRVHSLSYKGGFLGFDQTQPS 92
           V G P L++VP N+        SPF     + D    +    ++L  +G F+GF+ T+  
Sbjct: 39  VNGHPFLTQVPPNITTTTTSTPSPFLDFKSNKDT---IANNNNTLQQQGCFVGFNTTEAK 98

Query: 93  DRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVML--NIPEIKSYVVII 152
                 LGK KG +F S+FRFK WW+T WVG +G +LQ ETQ ++L  NI   + YV+++
Sbjct: 99  SHHVVPLGKLKGIKFTSIFRFKVWWTTHWVGTNGHELQHETQILILDKNISLGRPYVLLL 158

Query: 153 PIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFDAIAYVHVSDNPYRLMKEAYAAVRV 212
           PI+E SFR+++ PG +  V +  ESGSTHV  S+F A  Y+H+S++PYRL+KEA   ++ 
Sbjct: 159 PILENSFRTSLQPGLNDYVDMSVESGSTHVTGSTFKACLYLHLSNDPYRLVKEAVKVIQT 218

Query: 213 HLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVGIWNGVSDFVEGGISPRFLIIDDGW 272
            L TF+ LEEK    +++KFGWCTWDAFYL V P G+W GV    +GG  P F+IIDDGW
Sbjct: 219 KLGTFKTLEEKTPPSIIEKFGWCTWDAFYLKVHPKGVWEGVKALTDGGCPPGFVIIDDGW 278

Query: 273 QSINLDGEDPT--RDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLTGPNAPSFDPKKP 332
           QSI+ D +DP   RD  N    G QM  RL +++E  KFR+Y+ G   G           
Sbjct: 279 QSISHDDDDPVTERDGMNRTSAGEQMPCRLIKYEENYKFREYENGDNGG----------- 338

Query: 333 KLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKLKEELHGIFGKEEEEESSAINKG 392
                                                                       
Sbjct: 339 ------------------------------------------------------------ 398

Query: 393 CTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGATHL-NSKIVPCKL 452
                      G+  F RDL+ +F+ ++ ++VWHAL G WGGVRP    +  +K+V  KL
Sbjct: 399 ---------KKGLVGFVRDLKEEFRSVESVYVWHALCGYWGGVRPKVCGMPEAKVVVPKL 438

Query: 453 SPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMHVSILKT 505
           SPG+  TM DLAV KI+E  +GLV P+ A + FD +HS+L   GI GVKVDV+H+  L +
Sbjct: 459 SPGVKMTMEDLAVDKIVENGVGLVPPNLAQEMFDGIHSHLESAGIDGVKVDVIHLLELLS 438

[Close]

BLAST of Cucsat.G3354 vs. ExPASy Swiss-Prot
Match: Q9FND9 (Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=RFS5 PE=1 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 5.2e-90
Identity = 190/498 (38.15%), Postives = 256/498 (51.41%), Query Frame = 0


Query: 16  KSDGLENLIDFS------DGKISVKGVPVLSEVPTNVFFSPFSSISQSSDAPLPLLQRVH 75
           KSD   N +DF+      D  +   G  VL++VP NV  +    +      PL +     
Sbjct: 8   KSDSGINGVDFTEKFRLEDSTLLANGQVVLTDVPVNVTLTSSPYLVDKDGVPLDV----- 67

Query: 76  SLSYKGGFLGFD-QTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQ 135
                G F+GF+   +P      S+GK K   F+S+FRFK WW+T WVG++G D++ ETQ
Sbjct: 68  ---SAGSFIGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQ 127

Query: 136 WVMLNIPEIKS---------YVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSF 195
            ++L+     S         YV+++P++EGSFRS+   G D  V +C ESGST V  S F
Sbjct: 128 IIILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEF 187

Query: 196 DAIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPV 255
             I YVH  D+P++L+K+A   +RVH+NTF+LLEEK    +VDKFGWCTWDAFYLTV+P 
Sbjct: 188 RQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPD 247

Query: 256 GIWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECE 315
           G+  GV   V+GG  P  ++IDDGWQSI  D +    +  N+ + G QM  RL +F+E  
Sbjct: 248 GVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENH 307

Query: 316 KFRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQK 375
           KF+ Y              PK                                       
Sbjct: 308 KFKDY------------VSPK--------------------------------------- 367

Query: 376 LKEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALA 435
                                          ++ GMKAF RDL+ +F  +D I+VWHAL 
Sbjct: 368 -----------------------------DQNDVGMKAFVRDLKDEFSTVDYIYVWHALC 417

Query: 436 GAWGGVRPGATHL-NSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMH 495
           G WGG+RP A  L  S I+  +LSPGL  TM DLAV KIIE  IG   PD A +F++ +H
Sbjct: 428 GYWGGLRPEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLH 417

Query: 496 SYLSKVGITGVKVDVMHV 497
           S+L   GI GVKVDV+H+
Sbjct: 488 SHLQNAGIDGVKVDVIHI 417

[Close]

BLAST of Cucsat.G3354 vs. ExPASy Swiss-Prot
Match: Q5VQG4 (Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=RFS PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.1e-84
Identity = 184/500 (36.80%), Postives = 253/500 (50.60%), Query Frame = 0


Query: 10  LNASVLKSDGLENLIDFS-DGK-ISVKGVPVLSEVPTNVFFSPFSSISQSSDAPLPLLQR 69
           L   V+  DGL     F+  GK ++V G P L +VP N+  +P S++  +SD P      
Sbjct: 12  LIGDVVAVDGLIKPPRFTLKGKDLAVDGHPFLLDVPANIRLTPASTLVPNSDVP------ 71

Query: 70  VHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMET 129
               +  G FLGFD     DR    +GK +   F+S+FRFK WW+T WVG +G D++ ET
Sbjct: 72  ---AAAAGSFLGFDAPAAKDRHVVPIGKLRDTRFMSIFRFKVWWTTHWVGTNGRDVENET 131

Query: 130 QWVMLNIPEIKS-------YVVIIPIIEGSFRSAMHPG-TDGQVLICAESGSTHVKTSSF 189
           Q ++L+    KS       YV+++PI+EG FR+ +  G  +  V +  ESGS+ V+ S F
Sbjct: 132 QMMILDQSGTKSSPTGPRPYVLLLPIVEGPFRACLESGKAEDYVHMVLESGSSTVRGSVF 191

Query: 190 DAIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPV 249
            +  Y+H  D+P+ L+K+A   VR HL TFRL+EEK    +VDKFGWCTWDAFYL V P 
Sbjct: 192 RSAVYLHAGDDPFDLVKDAMRVVRAHLGTFRLMEEKTPPPIVDKFGWCTWDAFYLKVHPE 251

Query: 250 GIWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAK--NLVLGGTQMTARLYRFDE 309
           G+W GV    +GG  P  ++IDDGWQSI  D +D    A+  N    G QM  RL +F E
Sbjct: 252 GVWEGVRRLADGGCPPGLVLIDDGWQSICHDDDDLGSGAEGMNRTSAGEQMPCRLIKFQE 311

Query: 310 CEKFRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKI 369
             KFR+YKG                                                   
Sbjct: 312 NYKFREYKG--------------------------------------------------- 371

Query: 370 QKLKEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHA 429
                                               GM  F R+++  F  ++ ++VWHA
Sbjct: 372 ------------------------------------GMGGFVREMKAAFPTVEQVYVWHA 415

Query: 430 LAGAWGGVRPGATHL-NSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDS 489
           L G WGG+RPGA  L  +K+V  +LSPGL  TM DLAV KI+   +GLV P +A + ++ 
Sbjct: 432 LCGYWGGLRPGAPGLPPAKVVAPRLSPGLQRTMEDLAVDKIVNNGVGLVDPRRARELYEG 415

Query: 490 MHSYLSKVGITGVKVDVMHV 497
           +HS+L   GI GVKVDV+H+
Sbjct: 492 LHSHLQASGIDGVKVDVIHL 415

[Close]

BLAST of Cucsat.G3354 vs. NCBI nr
Match: ABV44498.1 (stachyose synthetase variant 1 [Cucumis sativus] >ABV44499.1 stachyose synthetase variant 2 [Cucumis sativus] >ABV44500.1 stachyose synthetase variant 3 [Cucumis sativus])

HSP 1 Score: 1009 bits (2608), Expect = 0.0
Identity = 495/495 (100.00%), Postives = 495/495 (100.00%), Query Frame = 0


Query: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180
           SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360
           FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMH 495
           LSKVGITGVKVDVMH
Sbjct: 481 LSKVGITGVKVDVMH 495

[Close]

BLAST of Cucsat.G3354 vs. NCBI nr
Match: NP_001267675.1 (steryl-sulfatase [Cucumis sativus] >ABQ53598.1 stachyose synthase [Cucumis sativus])

HSP 1 Score: 1009 bits (2608), Expect = 0.0
Identity = 495/495 (100.00%), Postives = 495/495 (100.00%), Query Frame = 0


Query: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180
           SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360
           FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMH 495
           LSKVGITGVKVDVMH
Sbjct: 481 LSKVGITGVKVDVMH 495

[Close]

BLAST of Cucsat.G3354 vs. NCBI nr
Match: KAA0046706.1 (stachyose synthase [Cucumis melo var. makuwa] >TYK18242.1 stachyose synthase [Cucumis melo var. makuwa])

HSP 1 Score: 992 bits (2564), Expect = 0.0
Identity = 486/495 (98.18%), Postives = 490/495 (98.99%), Query Frame = 0


Query: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVP NVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPNNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180
           SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSA+HPGTDGQVLICAESGSTHVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180

Query: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGV+DFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360
           FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           KEEL  IFG EEEEES+AINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELLEIFGNEEEEESTAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPGATHLNSKI+PCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMH 495
           LSKVGITGVKVDVMH
Sbjct: 481 LSKVGITGVKVDVMH 495

[Close]

BLAST of Cucsat.G3354 vs. NCBI nr
Match: XP_008451468.1 (PREDICTED: stachyose synthase [Cucumis melo])

HSP 1 Score: 987 bits (2552), Expect = 0.0
Identity = 484/495 (97.78%), Postives = 488/495 (98.59%), Query Frame = 0


Query: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVP NVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPNNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180
           SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSA+HPGTDGQVLICAESGSTHVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180

Query: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGV+DFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLY FDECEK
Sbjct: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYSFDECEK 300

Query: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL
Sbjct: 301 FRKYKGGSLMGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           KEEL  IFG EEEEES+AINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELLEIFGNEEEEESTAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPGATHLNSKI+PCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMH 495
           LSKVGITGVKVDVMH
Sbjct: 481 LSKVGITGVKVDVMH 495

[Close]

BLAST of Cucsat.G3354 vs. NCBI nr
Match: XP_038897999.1 (stachyose synthase [Benincasa hispida])

HSP 1 Score: 973 bits (2515), Expect = 0.0
Identity = 471/495 (95.15%), Postives = 491/495 (99.19%), Query Frame = 0


Query: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKS+ LENLIDF DGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSESLENLIDFLDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGF++TQPSDRLTNSLGKFKGREFVS+FRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFNRTQPSDRLTNSLGKFKGREFVSIFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180
           SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSA+HPGTDGQVLICAESGSTHVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180

Query: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPY+LMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGV+DFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360
           FRKYKGGSL+GP+APSFDPKKPKLLIAKAIEIEHAEK+RDKAIGSGVT++SKFETKI+KL
Sbjct: 301 FRKYKGGSLSGPSAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDISKFETKIKKL 360

Query: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           KEELH IFGKEEEE+S+AI+KGCTSCSCKA+NSGMKAFTRDLRT+FKGLDDIFVWHALAG
Sbjct: 361 KEELHEIFGKEEEEDSTAISKGCTSCSCKAENSGMKAFTRDLRTQFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THLNSKI+PCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGSTHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMH 495
           LSKVGITGVKVDVMH
Sbjct: 481 LSKVGITGVKVDVMH 495

[Close]

BLAST of Cucsat.G3354 vs. ExPASy TrEMBL
Match: A8CM21 (Stachyose synthetase variant 1 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1009 bits (2608), Expect = 0.0
Identity = 495/495 (100.00%), Postives = 495/495 (100.00%), Query Frame = 0


Query: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180
           SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360
           FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMH 495
           LSKVGITGVKVDVMH
Sbjct: 481 LSKVGITGVKVDVMH 495

[Close]

BLAST of Cucsat.G3354 vs. ExPASy TrEMBL
Match: B8LG99 (Stachyose synthase OS=Cucumis sativus OX=3659 GN=Csa_7G407800 PE=2 SV=1)

HSP 1 Score: 1009 bits (2608), Expect = 0.0
Identity = 495/495 (100.00%), Postives = 495/495 (100.00%), Query Frame = 0


Query: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180
           SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360
           FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMH 495
           LSKVGITGVKVDVMH
Sbjct: 481 LSKVGITGVKVDVMH 495

[Close]

BLAST of Cucsat.G3354 vs. ExPASy TrEMBL
Match: A0A5A7TXN2 (Stachyose synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001820 PE=3 SV=1)

HSP 1 Score: 992 bits (2564), Expect = 0.0
Identity = 486/495 (98.18%), Postives = 490/495 (98.99%), Query Frame = 0


Query: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVP NVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPNNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180
           SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSA+HPGTDGQVLICAESGSTHVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180

Query: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGV+DFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360
           FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           KEEL  IFG EEEEES+AINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELLEIFGNEEEEESTAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPGATHLNSKI+PCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMH 495
           LSKVGITGVKVDVMH
Sbjct: 481 LSKVGITGVKVDVMH 495

[Close]

BLAST of Cucsat.G3354 vs. ExPASy TrEMBL
Match: A0A1S3BRI8 (stachyose synthase OS=Cucumis melo OX=3656 GN=LOC103492745 PE=3 SV=1)

HSP 1 Score: 987 bits (2552), Expect = 0.0
Identity = 484/495 (97.78%), Postives = 488/495 (98.59%), Query Frame = 0


Query: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVP NVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPNNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180
           SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSA+HPGTDGQVLICAESGSTHVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180

Query: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGV+DFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLY FDECEK
Sbjct: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYSFDECEK 300

Query: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL
Sbjct: 301 FRKYKGGSLMGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           KEEL  IFG EEEEES+AINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELLEIFGNEEEEESTAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPGATHLNSKI+PCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMH 495
           LSKVGITGVKVDVMH
Sbjct: 481 LSKVGITGVKVDVMH 495

[Close]

BLAST of Cucsat.G3354 vs. ExPASy TrEMBL
Match: A0A6J1H730 (stachyose synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111461037 PE=3 SV=1)

HSP 1 Score: 919 bits (2375), Expect = 0.0
Identity = 440/495 (88.89%), Postives = 472/495 (95.35%), Query Frame = 0


Query: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDP++L +S LKSDGLENL+DF+DGK+SVKGV +LS+VP+NVFFS FSSI QSSDA
Sbjct: 1   MAPPNDPSSLTSSPLKSDGLENLVDFADGKLSVKGVSLLSDVPSNVFFSSFSSICQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVH+LS+KGGFLGFDQT+ SDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHTLSHKGGFLGFDQTKSSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180
           SDLQMETQW MLN+PEIKSYVV IPIIEGSFRSA+HPG DG V+ICAESGSTHVKTSSFD
Sbjct: 121 SDLQMETQWFMLNVPEIKSYVVFIPIIEGSFRSAIHPGVDGHVMICAESGSTHVKTSSFD 180

Query: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240
           AIAYVHVSDNPY+LMKEAYAA+RVH+NTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG
Sbjct: 181 AIAYVHVSDNPYKLMKEAYAAIRVHMNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGV+DF EGG+SPRFLIIDDGWQSIN+DGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVNDFAEGGVSPRFLIIDDGWQSINIDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360
           FRKYKGGSL+GPNAPSFDPKKPKLLIAKAIEIEHAEK+RDKAIGSGV +++KFETKI KL
Sbjct: 301 FRKYKGGSLSGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVADITKFETKIHKL 360

Query: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           KEEL+ IFGKE++        GC+SCSCK D+SGMKAFTRDLRTKFKGLDD+FVWHALAG
Sbjct: 361 KEELNAIFGKEQD-------SGCSSCSCKGDDSGMKAFTRDLRTKFKGLDDVFVWHALAG 420

Query: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMH 495
           LSKVGITGVKVDVMH
Sbjct: 481 LSKVGITGVKVDVMH 488

[Close]

BLAST of Cucsat.G3354 vs. TAIR 10
Match: AT4G01970.1 (stachyose synthase )

HSP 1 Score: 575.9 bits (1483), Expect = 3.3e-164
Identity = 283/475 (59.58%), Postives = 352/475 (74.11%), Query Frame = 0


Query: 22  NLIDFSDGKISVK-GVPVLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGF 81
           N  + S+G +  K   P+L +VP NV F+PFSS S S+DAPLP+L RV + ++KGGFLGF
Sbjct: 32  NSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGF 91

Query: 82  DQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVMLNIPEIKSY 141
            +  PSDRLTNSLG+F+ REF+S+FRFK WWST W+G SGSDLQ ETQWVML IPEI SY
Sbjct: 92  TKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVMLKIPEIDSY 151

Query: 142 VVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFDAIAYVHVSDNPYRLMKEAYA 201
           V IIP IEG+FR+++ PG  G VLICAESGST VK SSF +IAY+H+ DNPY LMKEA++
Sbjct: 152 VAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEAFS 211

Query: 202 AVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVGIWNGVSDFVEGGISPRFLII 261
           A+RVH+NTF+LLEEK +  +VDKFGWCTWDA YLTVDP  IW GV +F +GG+ P+F+II
Sbjct: 212 ALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFVII 271

Query: 262 DDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLTGPNAPSFDPK 321
           DDGWQSIN DG++  +DA+NLVLGG QMTARL  F EC+KFR YKGGS    +A  F+P 
Sbjct: 272 DDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPL 331

Query: 322 KPKLLIAKAIEIEHAEKERDKAI-GSGVTNVSKFETKIQKLKEELHGIFGKEEEEESSAI 381
           KPK+LI KA E   A   R K +  SG  ++++ + KI+ L EEL+ +F + E+EES   
Sbjct: 332 KPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLG- 391

Query: 382 NKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGA-THLNSKIVP 441
                  S     SGM AFT+DLR +FK LDDI+VWHAL GAW GVRP     L +K+ P
Sbjct: 392 -------SDDVSGSGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKVAP 451

Query: 442 CKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDV 494
            +LSP L  TM DLAV K++E  IGLVHP +A +F+DSMHSYL+ VG+TG K+DV
Sbjct: 452 FELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDV 498

[Close]

BLAST of Cucsat.G3354 vs. TAIR 10
Match: AT5G40390.1 (Raffinose synthase family protein )

HSP 1 Score: 333.2 bits (853), Expect = 3.7e-91
Identity = 190/498 (38.15%), Postives = 256/498 (51.41%), Query Frame = 0


Query: 16  KSDGLENLIDFS------DGKISVKGVPVLSEVPTNVFFSPFSSISQSSDAPLPLLQRVH 75
           KSD   N +DF+      D  +   G  VL++VP NV  +    +      PL +     
Sbjct: 8   KSDSGINGVDFTEKFRLEDSTLLANGQVVLTDVPVNVTLTSSPYLVDKDGVPLDV----- 67

Query: 76  SLSYKGGFLGFD-QTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQ 135
                G F+GF+   +P      S+GK K   F+S+FRFK WW+T WVG++G D++ ETQ
Sbjct: 68  ---SAGSFIGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQ 127

Query: 136 WVMLNIPEIKS---------YVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSF 195
            ++L+     S         YV+++P++EGSFRS+   G D  V +C ESGST V  S F
Sbjct: 128 IIILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEF 187

Query: 196 DAIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPV 255
             I YVH  D+P++L+K+A   +RVH+NTF+LLEEK    +VDKFGWCTWDAFYLTV+P 
Sbjct: 188 RQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPD 247

Query: 256 GIWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECE 315
           G+  GV   V+GG  P  ++IDDGWQSI  D +    +  N+ + G QM  RL +F+E  
Sbjct: 248 GVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENH 307

Query: 316 KFRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQK 375
           KF+ Y              PK                                       
Sbjct: 308 KFKDY------------VSPK--------------------------------------- 367

Query: 376 LKEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALA 435
                                          ++ GMKAF RDL+ +F  +D I+VWHAL 
Sbjct: 368 -----------------------------DQNDVGMKAFVRDLKDEFSTVDYIYVWHALC 417

Query: 436 GAWGGVRPGATHL-NSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMH 495
           G WGG+RP A  L  S I+  +LSPGL  TM DLAV KIIE  IG   PD A +F++ +H
Sbjct: 428 GYWGGLRPEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLH 417

Query: 496 SYLSKVGITGVKVDVMHV 497
           S+L   GI GVKVDV+H+
Sbjct: 488 SHLQNAGIDGVKVDVIHI 417

[Close]

BLAST of Cucsat.G3354 vs. TAIR 10
Match: AT3G57520.1 (seed imbibition 2 )

HSP 1 Score: 221.1 bits (562), Expect = 2.1e-57
Identity = 137/485 (28.25%), Postives = 214/485 (44.12%), Query Frame = 0


Query: 24  IDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGFDQT 83
           I   +  + V+G  +L+++P N+  +P +     S                G F+G    
Sbjct: 7   ISVQNDNLVVQGKTILTKIPDNIILTPVTGNGFVS----------------GSFIGATFE 66

Query: 84  QPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVML-NIPEIKS--- 143
           Q        +G  +G  F+  FRFK WW T  +G+ G D+ +ETQ+++L +  E++    
Sbjct: 67  QSKSLHVFPIGVLEGLRFMCCFRFKLWWMTQRMGSCGKDIPLETQFMLLESKDEVEGNGD 126

Query: 144 -----YVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFDAIAYVHVSDNPYRL 203
                Y V +P++EG FR+ +      ++ IC ESG   V+TS    + YVH   NP+ +
Sbjct: 127 DAPTVYTVFLPLLEGQFRAVLQGNEKNEIEICFESGDKAVETSQGTHLVYVHAGTNPFEV 186

Query: 204 MKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVGIWNGVSDFVEGGIS 263
           ++++  AV  H+ TF   E+K +   +D FGWCTWDAFY  V   G+  G+    EGG  
Sbjct: 187 IRQSVKAVERHMQTFHHREKKKLPSFLDWFGWCTWDAFYTDVTAEGVDEGLKSLSEGGTP 246

Query: 264 PRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLTGPNA 323
           P+FLIIDDGWQ I    E+  +D   +V  G Q   RL    E  KF+K           
Sbjct: 247 PKFLIIDDGWQQI----ENKEKDENCVVQEGAQFATRLVGIKENAKFQK----------- 306

Query: 324 PSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKLKEELHGIFGKEEEE 383
                                  ++D       T VS  ++ +   K+            
Sbjct: 307 ---------------------SDQKD-------TQVSGLKSVVDNAKQR----------- 366

Query: 384 ESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGAT---H 443
                                             +  ++ WHALAG WGGV+P A+   H
Sbjct: 367 --------------------------------HNVKQVYAWHALAGYWGGVKPAASGMEH 389

Query: 444 LNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKV 497
            +S +     SPG+ G   D+ +  +    +GLV+P +  +F++ +HSYL+  GI GVKV
Sbjct: 427 YDSALAYPVQSPGVLGNQPDIVMDSLAVHGLGLVNPKKVFNFYNELHSYLASCGIDGVKV 389

[Close]

BLAST of Cucsat.G3354 vs. TAIR 10
Match: AT3G57520.3 (seed imbibition 2 )

HSP 1 Score: 221.1 bits (562), Expect = 2.1e-57
Identity = 137/485 (28.25%), Postives = 214/485 (44.12%), Query Frame = 0


Query: 24  IDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGFDQT 83
           I   +  + V+G  +L+++P N+  +P +     S                G F+G    
Sbjct: 7   ISVQNDNLVVQGKTILTKIPDNIILTPVTGNGFVS----------------GSFIGATFE 66

Query: 84  QPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVML-NIPEIKS--- 143
           Q        +G  +G  F+  FRFK WW T  +G+ G D+ +ETQ+++L +  E++    
Sbjct: 67  QSKSLHVFPIGVLEGLRFMCCFRFKLWWMTQRMGSCGKDIPLETQFMLLESKDEVEGNGD 126

Query: 144 -----YVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFDAIAYVHVSDNPYRL 203
                Y V +P++EG FR+ +      ++ IC ESG   V+TS    + YVH   NP+ +
Sbjct: 127 DAPTVYTVFLPLLEGQFRAVLQGNEKNEIEICFESGDKAVETSQGTHLVYVHAGTNPFEV 186

Query: 204 MKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVGIWNGVSDFVEGGIS 263
           ++++  AV  H+ TF   E+K +   +D FGWCTWDAFY  V   G+  G+    EGG  
Sbjct: 187 IRQSVKAVERHMQTFHHREKKKLPSFLDWFGWCTWDAFYTDVTAEGVDEGLKSLSEGGTP 246

Query: 264 PRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLTGPNA 323
           P+FLIIDDGWQ I    E+  +D   +V  G Q   RL    E  KF+K           
Sbjct: 247 PKFLIIDDGWQQI----ENKEKDENCVVQEGAQFATRLVGIKENAKFQK----------- 306

Query: 324 PSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKLKEELHGIFGKEEEE 383
                                  ++D       T VS  ++ +   K+            
Sbjct: 307 ---------------------SDQKD-------TQVSGLKSVVDNAKQR----------- 366

Query: 384 ESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGAT---H 443
                                             +  ++ WHALAG WGGV+P A+   H
Sbjct: 367 --------------------------------HNVKQVYAWHALAGYWGGVKPAASGMEH 389

Query: 444 LNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKV 497
            +S +     SPG+ G   D+ +  +    +GLV+P +  +F++ +HSYL+  GI GVKV
Sbjct: 427 YDSALAYPVQSPGVLGNQPDIVMDSLAVHGLGLVNPKKVFNFYNELHSYLASCGIDGVKV 389

[Close]

BLAST of Cucsat.G3354 vs. TAIR 10
Match: AT3G57520.2 (seed imbibition 2 )

HSP 1 Score: 221.1 bits (562), Expect = 2.1e-57
Identity = 137/485 (28.25%), Postives = 214/485 (44.12%), Query Frame = 0


Query: 24  IDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGFDQT 83
           I   +  + V+G  +L+++P N+  +P +     S                G F+G    
Sbjct: 7   ISVQNDNLVVQGKTILTKIPDNIILTPVTGNGFVS----------------GSFIGATFE 66

Query: 84  QPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVML-NIPEIKS--- 143
           Q        +G  +G  F+  FRFK WW T  +G+ G D+ +ETQ+++L +  E++    
Sbjct: 67  QSKSLHVFPIGVLEGLRFMCCFRFKLWWMTQRMGSCGKDIPLETQFMLLESKDEVEGNGD 126

Query: 144 -----YVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFDAIAYVHVSDNPYRL 203
                Y V +P++EG FR+ +      ++ IC ESG   V+TS    + YVH   NP+ +
Sbjct: 127 DAPTVYTVFLPLLEGQFRAVLQGNEKNEIEICFESGDKAVETSQGTHLVYVHAGTNPFEV 186

Query: 204 MKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVGIWNGVSDFVEGGIS 263
           ++++  AV  H+ TF   E+K +   +D FGWCTWDAFY  V   G+  G+    EGG  
Sbjct: 187 IRQSVKAVERHMQTFHHREKKKLPSFLDWFGWCTWDAFYTDVTAEGVDEGLKSLSEGGTP 246

Query: 264 PRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLTGPNA 323
           P+FLIIDDGWQ I    E+  +D   +V  G Q   RL    E  KF+K           
Sbjct: 247 PKFLIIDDGWQQI----ENKEKDENCVVQEGAQFATRLVGIKENAKFQK----------- 306

Query: 324 PSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKLKEELHGIFGKEEEE 383
                                  ++D       T VS  ++ +   K+            
Sbjct: 307 ---------------------SDQKD-------TQVSGLKSVVDNAKQR----------- 366

Query: 384 ESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGAT---H 443
                                             +  ++ WHALAG WGGV+P A+   H
Sbjct: 367 --------------------------------HNVKQVYAWHALAGYWGGVKPAASGMEH 389

Query: 444 LNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKV 497
            +S +     SPG+ G   D+ +  +    +GLV+P +  +F++ +HSYL+  GI GVKV
Sbjct: 427 YDSALAYPVQSPGVLGNQPDIVMDSLAVHGLGLVNPKKVFNFYNELHSYLASCGIDGVKV 389

The following BLAST results are available for this feature:

BLAST of Cucsat.G3354 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93XK2	1.6e-176	60.36	Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1	[more]
Q9SYJ4	4.6e-163	59.58	Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=...	[more]
Q8VWN6	5.6e-92	38.10	Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 S...	[more]
Q9FND9	5.2e-90	38.15	Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=...	[more]
Q5VQG4	1.1e-84	36.80	Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=399...	[more]

BLAST of Cucsat.G3354 vs. NCBI nr


Match Name	Identity	Description
ABV44498.1	100.00	stachyose synthetase variant 1 [Cucumis sativus] >ABV44499.1 stachyose synthetas...	[more]
NP_001267675.1	100.00	steryl-sulfatase [Cucumis sativus] >ABQ53598.1 stachyose synthase [Cucumis sativ...	[more]
KAA0046706.1	98.18	stachyose synthase [Cucumis melo var. makuwa] >TYK18242.1 stachyose synthase [Cu...	[more]
XP_008451468.1	97.78	PREDICTED: stachyose synthase [Cucumis melo]	[more]
XP_038897999.1	95.15	stachyose synthase [Benincasa hispida]	[more]

BLAST of Cucsat.G3354 vs. ExPASy TrEMBL


Match Name	Identity	Description
A8CM21	100.00	Stachyose synthetase variant 1 OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
B8LG99	100.00	Stachyose synthase OS=Cucumis sativus OX=3659 GN=Csa_7G407800 PE=2 SV=1	[more]
A0A5A7TXN2	98.18	Stachyose synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G0...	[more]
A0A1S3BRI8	97.78	stachyose synthase OS=Cucumis melo OX=3656 GN=LOC103492745 PE=3 SV=1	[more]
A0A6J1H730	88.89	stachyose synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111461037 PE=3 SV=1	[more]

BLAST of Cucsat.G3354 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G01970.1	3.3e-164	59.58	stachyose synthase	[more]
AT5G40390.1	3.7e-91	38.15	Raffinose synthase family protein	[more]
AT3G57520.1	2.1e-57	28.25	seed imbibition 2	[more]
AT3G57520.3	2.1e-57	28.25	seed imbibition 2	[more]
AT3G57520.2	2.1e-57	28.25	seed imbibition 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008811	Glycosyl hydrolases 36	PFAM	PF05691	Raffinose_syn	coord: 26..496 e-value: 2.7E-153 score: 511.6
IPR008811	Glycosyl hydrolases 36	PANTHER	PTHR31268	FAMILY NOT NAMED	coord: 1..495
None	No IPR available	PANTHER	PTHR31268:SF8	GALACTINOL--SUCROSE GALACTOSYLTRANSFERASE 4-RELATED	coord: 1..495
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 220..499

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G3354.T3	Cucsat.G3354.T3	mRNA
Cucsat.G3354.T2	Cucsat.G3354.T2	mRNA
Cucsat.G3354.T5	Cucsat.G3354.T5	mRNA
Cucsat.G3354.T4	Cucsat.G3354.T4	mRNA
Cucsat.G3354.T6	Cucsat.G3354.T6	mRNA
Cucsat.G3354.T1	Cucsat.G3354.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0003824	catalytic activity