Cucsat.G243 (gene) Cucumber (B10) v3

Overview
NameCucsat.G243
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionMACPF domain-containing protein
Locationctg1: 5460767 .. 5468648 (-)
RNA-Seq ExpressionCucsat.G243
SyntenyCucsat.G243
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCGGAAAGTTTGACGGACGACTTGCTTCTCTCTCACGTGTCCATCTAAACTCTTTCGTCAAACCCTCCAAACAAGAATATATATTTTCATTTATTTTTGTTTTTCCCTTTTTCATTTTCCATTTTTGATATTTCTTCGCTTCTAGGGCTCCCTCGAACTTTACTTCTCTTTACTACCTGCTTTCTTCTCCTCTCAATTTCCATCTCCCCTTAGGGCTCTCTTTCATCTCTTCTAACCTCAACTCTCCAATGTAACCATTCTCACAATCACGCTTCTCTCTCTCTTTCTCTTTTTGGACTTTTCTGCTTCTTAACTTTGCTTCTTTCTCCATTTCTGTCCAATTTTATTCTGCTCTTCACCTTTTCCTTGTATTTTATTTTTACTTACTTGTTCATTTGAGCTTCTTATTCCTCCATTTTTGGGGATCGTCAACGATCTTTCTTTATCACAACCTTAACTGAAGTATAATTGCTGGAGTTTTGGATTTCGTGATGATAGTATGAGCTGTATAAATGGTGTTTTGGATCAATGGGATTCTATTTTCTGTTGTGTTAATGGAGATTTGGATCTAGCTTATTTCTTTGAGCTGGATATGTCTCCGCCTCGTATGTCGGGTCTTATTATTGTTGTTATTAATTTTTTTTTTTTTTTTTTTTTTTTAAACGTTCCTTCTTGTTTCAGCTATGTATTTATCTAGCTGGTGTTTGCTGCTTACTGTGTAGGGTTCATATGGCCTAGGTCAAGGGTGATGTTTGCTTGAGTTATGGCGCTTAAAGTTCATCCTTCCAAGGCAGCCGAGATAGCCATTGGGTCTATTGGTCGTGGATATGACATAACCATAGATCTAAGACTCAAGTATTGTAAAGGGAATTTGCATGGTTCTCGGTTGATAGAAATTGATGAAGGTGTGGGTCATGAGATTGTTCTTCCTGGTGGGATTTTAATCTCAAATGTGCCCAAATCCATTAAGTGTGACAAAGGGGAGCGAACGAGGTTCAGGTCTGATGTTCTCTCTTTCCAACAGGTATTGCACTTAATGTTAATGCTTGGATAGTTTTGGATCTCGTACTATGATTTTCTTCTCAATAGCTTTGGCACGTTTGTGCTGTAGTAGTGTATGACTCTGGGCAATGACTTATTGCTGGTAAATACGTTTGGCCTTTGACTCTGTGGAAATGTAGATAGATTGTTGGGTGAGGAGGAATGAAAGAACAGGTGAATTTTATTTTATCCTTTACCCTTAAAATTGCATCCCCTGAGAAACCACTGTCGTTAACTGGTTAGAAACCACATGTTCTGATTCGACCACAGCAACACAGTTGGCTAGGACAACTAAAATTTTCACGGGCAAGAGTTAACTGTTCCCAGTCATTTTGTTTCTGCTGTATGGGCTGTGAACCACCTCCCTTCCCCATGATCTAACATTGTAAAATATAGTTGATGTGCAATTCTGCAAACCATACTTTTCTGTTCTTGTATGTGATAGGAAATAATAGTATATCAATCAAATTATGCTACCCAATTTAGATAACAGATATTATCCAGCAATCCCAGTTCAGATTCGAATTCCAATATACAGCGGAATAATCAAATTGAAATTCGTAAGCTTAAAAGAATAGACAAAATAATCTAAACTTCAAAGCAATAAGATCAATACTAAACCAAGTTTTAGAGAAGAGAGGTGACACCAATTTGTACTGGTTCACTTTGTGTGGGTTACATCCAGTTCTCTGCTACCAATGCAAGTTTCACTAAGATGATTTTGGAGAAGAACCCAAGAGTTTATCAAAGAATAATTTCTCTTAATTTTGTCTCAAAGAAGAATGCTCTTAAATCACTCTTAGTCTCTTAGAATATCATTCCTACTCAATGGAGAGAACACACACATACAAAAGTCAAGAAAAACTCTCAAAACAATCAAGATTTTTCTTCTTCTTTATTGTGAAAGACACCCAACGGCAGAGAGCAGAAAAGAAAACAAACACTACTGCCTTGCTAACAATTTTTTCTCTCTGATTTCTGACGGCAACCACCCATGAAGCAGTTTTTCTTCTTCTTTATTTTCAGTTACCAAAAAACAAGTGGGCCTTTTGTTTTGGGCTCCAAAATACATAACAGTATGTTCTATCAATGTCTACTGACCATATAGCATTAAAAATTTATAATGCAGATGTCAGAGCAGTTTAATCAGGAGATGACTTTGACTGGTAAAATTCCTTCAGGTCACTTCAACACCATGTTTGAGTTTTCAGGATGTTGGCAGAAAGATGCAGCCAATACAAAGAACCTAGCTTTTGATGGGGTCTTCATTACCCTGTACACGGTTGCTTTGGAAAAATCACAAATGGTACTTTGCGATCACGTAAGGAAAGCTGTGCCATCATCTTGGGACCCTGCTGCACTTGCAAGGTGTATTCTTTTTTACTAGATACATAACATATACATACATATATGCCAGATACAGAGCCAATACAAATATACATTTTTTTTCTTTTCTTTATTACCTCCTTATGAAGTGCAATTGTGCTTAGTTTCTTCTATTTCACACTCCTATAATTTATTTTAGTTCTACTAGATTAGTGGAGAACAACCTTCAAAAAATAAAAAAAGGGTTTAGTGGAGAGAACAAGCTATATGTCCTTGAGTTGAGTGATCGATTCAATAATTAATTTAACTATTTGATTTAAAAGGACGGGGGAGTTCTTAAGGGGGTGGAGGTTCTTGAAGGAGGCTTAAAACTTAACACGCTAAGAAAACAGAAAGAAGAAAAGAGTCTGATAAGAAGTGAGATAGTGATTCTTATTCAGACAAGAAACTTTCCCTAAGGAAAAATACAGAACGCACTTCCTCCCACCGTGGATAAGAAACATACTTCCAAATGAAATAACTGCCTACCTTCCTTAGAAACATCTCTCTACCTATAATGCCTACCTTTCCTAAATAACTTCCTACTGGCCTACATATACCCTTATTTCACTCTTTAACTAACTGTGGTTCCCATCTCCCTTGCTATTGGACGAGTTCACTCTCTCACTTACCTGTACATTCCTCTTTTTACCCTTATGTTGATATTAGAATATAATAGGAGGCCTAACAAAATAATGATATCTAATTTTGTATGTCAGGTTTCTGTGTTAAATTAATCAGGAGATCTTGCATTTATGCTCTCCTCTCTTGTTTTAGTAGTTAAAAAGCCTTATGACTGTTCTGCTTATCGGTCTCCATAATTAATTTTGGGGAAGTCTCCATAAGTTATGTCTTTGCTACCAACAGTTTCTAATAATGTGCTGCCAATGAATGATTGATATATCCTTTTTCTTTTGTTCCTGCCTTGAACAGTAGCTTCTTTCTAAGTCGTATTCTAACCACAGTAATTGTTGTTATCATCTGTGGCTAGAAACACTGATGTTTAAACAAGTTGAATTGTGTTTTTCACCTTTTTTGAGTTGGGGTAGTAAAATTAATCATTTTGCCTACGTAATTGTGTTAGGTTTATTGAAACCTTTGGCACCCACATTATTGTTGGCTTAAAGATGGGAGGAAAGGATGTGATATATATGAAGCAGCAGCATTCATCTACTCTTCAACCTTCTGAACCTTCTGAAGTGCAGAAGAGTTTGAAGGAGATGGCAGATAAAAGGTTTTCAGAGACCATTGCACAATATGGAATGGGTTCTGAACGACTTTACAACAATGACAAGGTTTCTGATATTTAGCATAATTTGTTTCTCAACCAATACTATTTTAGTTCTCGATGATTGCTAAATACTTGAAAACTGAAAAGGGTTTAGATTTTATTTTAGTTCTTGAACTTCCATATTTGTTCTGCTTTGGATTTTCATAAATAAAATTTAACGCCTGAGAAACTTGTCTTTGTCGAGCTTATTATCTTAGTTTGTTTAGAAAAACAATGCATGATTATATTTTAATCATTCCTCCTACCCTCGTTGGGTTTATAGCCGTGATTTTATTTTATTCCTTCGTAGATTACTTTATATATTAATAAATCATACTCTTTAAAAAAAAGAAAAAGAAAAAACTGTGAATGGCATCTGTTTTTGTTTGCTAATTAACCAAATAATACATTCTTTATATATTTTGTTGACTCCTGGATTAACTGTTATTGTTTTTCACTCATTAAAGTACAAGATTTACAAATCCATTCAGTAAACTGATTCTGGTGTTTTTCTTTTCTTTTTGTCAAACGGAAACAAAACTTTACATTGAAGAAATCTCAGAGTACAGTGAAACAGCATGATAAGGGAAATGCAACAAAAAAGAAACCCAAAGCTGGACTTATCCTGAAAATTAATGCAAATACAATCATATACAACCCTAGAGAGAACAAATTGAGATCTTGAATGAAAATTTCAAATGGTGTTCTTATTCATTGTGATTGAATTTCGATTTAAAAGTAATGGACTTCCCATCCATATATTGCCCAAAGTTTTTTGTGATCTTGTTGCTCATGAACGGCATTCTTTTCGAAGTTTTCATCTAAATTTATTCATATTCTGTTTCAGATTGAAATTAGGGAGCAACATTTGAGGTTTGGCGATGCTAGCTCTGCCATTTCTCCACTGAAGGTGATATAAGCTGGAAAATTTATTTATCAATTTGGGAAAATGCCAAGAGTAGTGTTAGAAGCACCCGTCCTCTCTCAAATACAGCTGCTCTTAGAATACTAGCCAAAAGATAACTATACCACCACTAAACTGAGAATATATCTGACCCCTAAAAAAAATGTTCCCTTGGACTTACTTTTAACGAACTTCTAACAGAAATCTATTCTCAATTCTCTATTCTCCCCCTTTCTTCTTTTATGCATGTATAAATAATAGATTAGGAGGTCTATCAGGAGGCGAAATTCATTGAGTTATTTTGTTGGGAGGGTTGTTGGATATCTTTACAACTCTTACTTTTATATTAATCATTACACTCACAAGCGATGAGAAAATTTTGGGTTATTTTGACATTTTCTAGTAAATCAATTTGCTTGCCAGGATATTGTACCCATTTGCAAGAGAAGAGGTGGAAGTGACAGGATTAGCCTCCCTCATCACGAGTGGTTGCAGACAGTTCAGTTGGAGCCAGACGTGATATCAATGTCCTTTATTCCAATTACTTCTTTATTGAATGGAGTTCCAGGAAGTGGATTCTTGAGCCATGCAATTAATCTTTATTTGCGATGTGAGTAAAAAATACATTCTGCTTGTAAAGTTACAATAGATTGTGCTAGCTTATCAATGTGCCTTGTTTATATACAAAAGGAGTCTGTATAAAGGCACTATAAGGAAAGGGCTCCGTGGTTGAGAATAAAAGACGGACCAATAATTATAAAAATCCAACAAAGTAACCCTAGAGAGAGGCTAGAAACCTAAGTGACTATAAATTGTATTCTTATTTGTTTAGGTTTTCTAAATTTATTTGGATTTAACTTACTGTATGGAAGAGCTTTATTGGGGCTCATGTGTTGTAGGGAATGGTAGAAAATTATCAGCAAGAAATACTTGATCTAGAAAAGCTCCCTTTGAAATTTACTCAAGTTGGGTGATCTTTTGTAAACTCCAATGTGGGGTTTGAACCTTTGATGACTCCTCCCCTCTACATTAATGGAAAGGACGTTTATTGTTTCAAAATAGGTTCCAAGAAAGACAGGAGATAAAATGCTGCTTTAGTCGGGAAACCCATATTATACTTTACTTTGACACAAATAAATTTGTTTCTCTTTCACCTTGATCATTGCAATACGTGCATATACATACTGAAATAAGCTTCGTATCTCAAGGTGTTCAATTACATTTTTTCATACATCCTTTTCATTATTGCAGACAAACCACCAATTGAAGAGCTTCATCAATTTTTGGAGTTTCAGCTCCCAAGGCAATGGGCGCCAATTTTCAGTGAGCTTCCACTTGGTCCACAACGGAAACAACACAACTTGGCATCTCTACAGTTTAGTCTTATGGGATCAAAGCTTTATGTGAATACTACTCCCGTATGGTTTCTTTTTCCTAAAGTTTTTAGTTTCTTCATACTGCAAATTGTGAATAATCTTCATTATGGTCTCTACTATCTTCTTTTATCATTCATAACTTAGGAGAGTTTTTTGGATCCATACGAAGACAGACAATAGGAAATATGATGTGATATAAGGGTGAAGTTCCATTAAATTTTCAATCTTTGCTGCACTAGGTTAGGCTACATTGGGGTATTCAACCGGTAACCTCTTTCACAGAAATACAGTCTTTTCCAGTTGACTTTGCTCAGTTTGGCAACATGCCTGAGTGTTTTCTGGTGTACGAATGCCTGTGGATCAAGAAATTAGTACGAAGTTTTGACTGTAATGTGGTATAAGACTCCTACTTTATGCCGGTGGTCTCTCAACCGTTGGTGTAGCACTGTGCTGGTTCATTGGACTTTTCAGAAAAGAACAGAAGCAATAGACTGTATTTCTCGTTGTAGAGTATAGCAGAATCTTTTCTTTAATAATAAAAAAAGAATTATAGAATCTTTGAAAATCTAACGTTAATTAAAAGAAACAATCTAGTTGACCCAAATTATCATCCCATTCCAGGGTTAATAATAATAAACAATGACGAATATAAGTGGTTTTGAGTTCAGTTTTTTACAAGCTTTAGAAGATATAACTGAAGACCATTTTTTATTCACTCGTGTGTTTGATCACAGGTTGATGTGGGCAGGAGGCCGGTTACAGGTCTCCGTCTTTATTTGGAAGGTAAAAGAAGCAACTGTTTATCCATCCACTTACAGCACCTCTCCTCTTTACCCAAGATTTTTCAACTTACGGAGGATCCAAATGGAAACTTCCGTCAGGACTCATATGATCGCAGATATCTTGAAAAAGTTCAATCCAAGTACTTTTCTCATGTATGCACTGCTCCTGTTGAAGCCGACGAGGACCTCTCAATAGTAACTGGGGCACAGTTGCAGGTTGTAAATCAAGGGATGAGAAATGTTCTGTTTCTACGACTTCGCTTTTCTTCAGTTTTGGGAGCAACGGTTGTAAGGCAGGCAGAGTGGGAAGGTTCACCTGGACTAGCAGCCAAGTCTGGACTTATATCAACACTAATCAGTCATCATTTCTCAACAGTAATGAAACCTCCTCCAAAACCAGCTGATGTGAATATAAATTCTGCTGTTTATCCTGGGGGTCCACCAGTACCTGTACAGGCCCCTAAGCTTCTGAAATTTGTAGATACCACTGAAATGAAAAGAGGACCCCAAGAGACTCCTGGGTATTGGGTTGTTTCTGGGGCAAGACTCATGGTGGAGAAGGGCAGGATCTCCCTTCGGGTTAAGTTTTCCTTACTAACCGTAATATTACCGGATGAAGAAGAAATCAATGATAATTAGCTCCTTTTGTTCATCCGTTTGATGTTAAGTGTGAAGGGAAGTTTAAAGACCGTCGAGAATATCGAGAATCAGCAGCAGGGTCTGAAAAGGAGGATTGGTCAGAATCAGAAAGAAGTCGGATCCCTTTCTTTTGGACAATAATCCAATTTAAGCCCAAGGAATGAAAGAACTCAAGGAGGGGTGAGACAATGTTGGATGATTCTGATGTGTATTGCGTTACAATTCGAACAAGGTCATCTATGTTGGTGATGTAATTTACATTTTGTGTAGGTATTGCGAATTCAACGCACTGGAGGTTTTCTTTTGCACCTTTTGACGAAGTATTTGTACATATTACATTTCCAGGTCTTCTGTGGATAAGTAGAGATTTCCAAATGTTTATTGTGTTTGTAGCCATTTATGTGAATGAGAGGGTTCATATAAACACTCTTTTAAATTGTTGAATTGTTGAATTTTGTGTACTGAAGTGAGGAAGGATGGTATTAGAGAGGGTTGTTATAATTTCCTTTCAGTTGCCCTTCCTTCTGAAGAGGTAAGTTGTAAACTTATATCCATAGTATAAAGTGAGTGCCATATAATATAATATATAAAATTCATTTTTATTTTGTTTTATTTTTACTTCGTA

Coding sequence (CDS)

ATGGCGCTTAAAGTTCATCCTTCCAAGGCAGCCGAGATAGCCATTGGGTCTATTGGTCGTGGATATGACATAACCATAGATCTAAGACTCAAGTATTGTAAAGGGAATTTGCATGGTTCTCGGTTGATAGAAATTGATGAAGGTGTGGGTCATGAGATTGTTCTTCCTGGTGGGATTTTAATCTCAAATGTGCCCAAATCCATTAAGTGTGACAAAGGGGAGCGAACGAGGTTCAGGTCTGATGTTCTCTCTTTCCAACAGATGTCAGAGCAGTTTAATCAGGAGATGACTTTGACTGGTAAAATTCCTTCAGGTCACTTCAACACCATGTTTGAGTTTTCAGGATGTTGGCAGAAAGATGCAGCCAATACAAAGAACCTAGCTTTTGATGGGGTCTTCATTACCCTGTACACGGTTGCTTTGGAAAAATCACAAATGGTACTTTGCGATCACGTAAGGAAAGCTGTGCCATCATCTTGGGACCCTGCTGCACTTGCAAGGTTTATTGAAACCTTTGGCACCCACATTATTGTTGGCTTAAAGATGGGAGGAAAGGATGTGATATATATGAAGCAGCAGCATTCATCTACTCTTCAACCTTCTGAACCTTCTGAAGTGCAGAAGAGTTTGAAGGAGATGGCAGATAAAAGGTTTTCAGAGACCATTGCACAATATGGAATGGGTTCTGAACGACTTTACAACAATGACAAGATTGAAATTAGGGAGCAACATTTGAGGTTTGGCGATGCTAGCTCTGCCATTTCTCCACTGAAGGATATTGTACCCATTTGCAAGAGAAGAGGTGGAAGTGACAGGATTAGCCTCCCTCATCACGAGTGGTTGCAGACAGTTCAGTTGGAGCCAGACGTGATATCAATGTCCTTTATTCCAATTACTTCTTTATTGAATGGAGTTCCAGGAAGTGGATTCTTGAGCCATGCAATTAATCTTTATTTGCGATACAAACCACCAATTGAAGAGCTTCATCAATTTTTGGAGTTTCAGCTCCCAAGGCAATGGGCGCCAATTTTCAGTGAGCTTCCACTTGGTCCACAACGGAAACAACACAACTTGGCATCTCTACAGTTTAGTCTTATGGGATCAAAGCTTTATGTGAATACTACTCCCGTTGATGTGGGCAGGAGGCCGGTTACAGGTCTCCGTCTTTATTTGGAAGGTAAAAGAAGCAACTGTTTATCCATCCACTTACAGCACCTCTCCTCTTTACCCAAGATTTTTCAACTTACGGAGGATCCAAATGGAAACTTCCGTCAGGACTCATATGATCGCAGATATCTTGAAAAAGTTCAATCCAAGTACTTTTCTCATGTATGCACTGCTCCTGTTGAAGCCGACGAGGACCTCTCAATAGTAACTGGGGCACAGTTGCAGGTTGTAAATCAAGGGATGAGAAATGTTCTGTTTCTACGACTTCGCTTTTCTTCAGTTTTGGGAGCAACGGTTGTAAGGCAGGCAGAGTGGGAAGGTTCACCTGGACTAGCAGCCAAGTCTGGACTTATATCAACACTAATCAGTCATCATTTCTCAACAGTAATGAAACCTCCTCCAAAACCAGCTGATGTGAATATAAATTCTGCTGTTTATCCTGGGGGTCCACCAGTACCTGTACAGGCCCCTAAGCTTCTGAAATTTGTAGATACCACTGAAATGAAAAGAGGACCCCAAGAGACTCCTGGGTATTGGGTTGTTTCTGGGGCAAGACTCATGGTGGAGAAGGGCAGGATCTCCCTTCGGGTTAAGTTTTCCTTACTAACCGTAATATTACCGGATGAAGAAGAAATCAATGATAATTAG

Protein sequence

MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEIREQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRFSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEINDN
Homology
BLAST of Cucsat.G243 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 1.8e-157
Identity = 278/380 (73.16%), Postives = 319/380 (83.95%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGH-EIVLPGGI 60
           MAL++  SKAAE+AIGSIG GYD+ IDLRLKYCKG    SRL++I EG  + EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  LISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
            I NV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DAA TKNLAFDGVFI+LY+VAL+KSQ++L +HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIE 240
           +KMGGKDVIY KQQHSS LQ   P ++QK LKE+ADKRF E    +  GSER+  + K+E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQ---PEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVE 240

Query: 241 IREQHLRFGDASS--AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIP 300
            +EQ LRF D SS  + +  +D V +CKRRGG+D  +L H+EWLQTVQ+EPDVISMSFIP
Sbjct: 241 TKEQRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 300

Query: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHN 360
           ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ +
Sbjct: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQS 360

Query: 361 LASLQFSLMGSKLYVNTTPV 378
            ASLQFS  G KLYVNTTPV
Sbjct: 361 CASLQFSFFGPKLYVNTTPV 377

BLAST of Cucsat.G243 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.7e-113
Identity = 209/377 (55.44%), Postives = 266/377 (70.56%), Query Frame = 0

Query: 10  AAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSIK 69
           AAE A+  IG GYD+  D+R   CK    GSRL+EID     +++ PGGI+++NV  SIK
Sbjct: 14  AAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK 73

Query: 70  CDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAF 129
           CDKGERTR RSD+LSF QMSE+FNQ+M L+GKIPSG FN MF FS CW KDA++ K LA+
Sbjct: 74  CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAY 133

Query: 130 DGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIY 189
           DG FI+LY+V + + Q+ L D V++ VPSSWD AALA FIE +GTH++VG+ MGGKDVI+
Sbjct: 134 DGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIH 193

Query: 190 MKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEIREQHLRFG- 249
           +KQ   S     EP E+QK LK   D+RF     +       +Y+    E  E  L++G 
Sbjct: 194 VKQMRKSN---HEPEEIQKMLKHWGDERFCVDPVE-SKSPASVYSGKPKE--ENLLQWGL 253

Query: 250 -----DASSAI---SPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 309
                  SSA+   +  ++I+ +C RRGG D +   H  WL TV   P+VISM F+PITS
Sbjct: 254 QPFGTSVSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITS 313

Query: 310 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 369
           LL+G+PG+GFLSHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R + +  S
Sbjct: 314 LLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPS 373

Query: 370 LQFSLMGSKLYVNTTPV 378
           LQFSLMG KLYVNT+ V
Sbjct: 374 LQFSLMGPKLYVNTSKV 383

BLAST of Cucsat.G243 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.1e-85
Identity = 173/383 (45.17%), Postives = 240/383 (62.66%), Query Frame = 0

Query: 12  EIAIGSIGRGYDITIDLRLKYCK---GNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSI 71
           E A+ S+G+G+D+T D RLKYCK   G+    RL+ +D+    E+ +PG  +  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEF-SGCWQKDAANTKNL 131
            CDKGERTRFRSD+L F +MSE FNQ  ++TGKIPSG+FN  F F SG W  DAAN K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 AFDGVFITLYTVALEK-SQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKD 191
             D   +TL+ + +   +++ L D VR AVPSSWDP  LARFIE +GTH+I G+ +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSET--IAQYGMGSERLYNNDKIEIREQH 251
           V+ ++Q  SS L   +   ++  L ++ D+ F+ +  ++   +     +++ + +  E  
Sbjct: 190 VVVVRQDKSSDL---DNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAF 249

Query: 252 LRFGDASS------AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPI 311
             F D  +      +I+    I  IC +RGG  R +  H EWL TV  +PD I+ +FIPI
Sbjct: 250 NVFDDKQTVAFNNFSINSQNGITVICAKRGGDGR-AKSHSEWLITVPDKPDAINFNFIPI 309

Query: 312 TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNL 371
           TSLL  VPGSG LSHA++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      N+
Sbjct: 310 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFG---AAPNM 369

Query: 372 AS----LQFSLMGSKLYVNTTPV 378
           AS    L  + MG KLYVNTTPV
Sbjct: 370 ASAYPALHINFMGPKLYVNTTPV 385

BLAST of Cucsat.G243 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.5e-82
Identity = 167/364 (45.88%), Postives = 222/364 (60.99%), Query Frame = 0

Query: 14  AIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSIKCDKG 73
           AI ++GRG+D+T D+RL YCKG   GSRL+ I+EG   ++ L  G L+ NVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKG-APGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAFDGVF 133
                R  V SF +M+E+FN    + G IP G FN MF ++G WQ DAA+TK+LA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 ITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIYMKQQ 193
           I LY V L K  +VL + +R+AVPSSWDPA+LA FIE +GTHI+  + +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEIREQHLRFGDASSA 253
            SS   P   SE++  + +M   RF E  +Q   G                L++ D    
Sbjct: 204 QSS---PLPVSEIENYVNDMIKHRFHEAESQSITGP---------------LKYKD---- 263

Query: 254 ISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSH 313
               KDI  I +RRGG D +   H  W +TV   PD+I+M+F PI SLL GVPG   L+ 
Sbjct: 264 ----KDITVIFRRRGGDD-LEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTR 323

Query: 314 AINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVN 373
           AI LYL YKPPIE+L  FL++Q+ R WAP  S L    QRK+   +SLQFSLMG KL+++
Sbjct: 324 AIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFIS 355

Query: 374 TTPV 378
              V
Sbjct: 384 ADQV 355

BLAST of Cucsat.G243 vs. NCBI nr
Match: XP_004133715.1 (MACPF domain-containing protein At4g24290 [Cucumis sativus] >XP_011650569.1 MACPF domain-containing protein At4g24290 [Cucumis sativus] >KAE8650184.1 hypothetical protein Csa_010735 [Cucumis sativus])

HSP 1 Score: 763 bits (1970), Expect = 4.75e-274
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240
           KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300
           REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS
Sbjct: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPV 377
           LQFSLMGSKLYVNTTPV
Sbjct: 361 LQFSLMGSKLYVNTTPV 377

BLAST of Cucsat.G243 vs. NCBI nr
Match: XP_008452249.1 (PREDICTED: MACPF domain-containing protein At4g24290 [Cucumis melo] >KAA0060601.1 MACPF domain-containing protein [Cucumis melo var. makuwa] >TYK02270.1 MACPF domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 759 bits (1960), Expect = 1.58e-272
Identity = 374/377 (99.20%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGI+
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVP+SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240
           KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300
           REQHLRFGDAS+AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS
Sbjct: 241 REQHLRFGDASTAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPV 377
           LQFSLMGSKLYVNTTPV
Sbjct: 361 LQFSLMGSKLYVNTTPV 377

BLAST of Cucsat.G243 vs. NCBI nr
Match: XP_038904757.1 (MACPF domain-containing protein At4g24290 [Benincasa hispida] >XP_038904758.1 MACPF domain-containing protein At4g24290 [Benincasa hispida])

HSP 1 Score: 751 bits (1939), Expect = 2.47e-269
Identity = 370/377 (98.14%), Postives = 374/377 (99.20%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAEIAI SIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL
Sbjct: 1   MALKVHPSKAAEIAIESIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSI+CDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIRCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240
           KMGGKDVIYMKQQHSST+QPSEPSEVQKSLKEMADKRFSE IAQYG GSER+Y+NDKIEI
Sbjct: 181 KMGGKDVIYMKQQHSSTIQPSEPSEVQKSLKEMADKRFSENIAQYGTGSERIYSNDKIEI 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300
           REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS
Sbjct: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPV 377
           LQFSLMGSKLYVNTTPV
Sbjct: 361 LQFSLMGSKLYVNTTPV 377

BLAST of Cucsat.G243 vs. NCBI nr
Match: XP_023540812.1 (MACPF domain-containing protein At4g24290 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 698 bits (1802), Expect = 1.57e-248
Identity = 350/378 (92.59%), Postives = 359/378 (94.97%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN + SRLIEIDEGVGH IVLPGGI 
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFNDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240
           KMGGKDVIYMKQQHSSTL   EPSEVQK LK+MADKRF+ET+ QYGMGSE +YNNDK E 
Sbjct: 181 KMGGKDVIYMKQQHSSTL---EPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNNDKFES 240

Query: 241 REQHLRFGDASSAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPIT 300
           REQHL+F DASSAIS LK DIV I KRRGGSDRI+LPHHEWLQTVQLEPDVIS+SFIPIT
Sbjct: 241 REQHLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPIT 300

Query: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLA 360

Query: 361 SLQFSLMGSKLYVNTTPV 377
           SLQFSLMGSKLYVNTTPV
Sbjct: 361 SLQFSLMGSKLYVNTTPV 375

BLAST of Cucsat.G243 vs. NCBI nr
Match: KAG7031360.1 (MACPF domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 696 bits (1797), Expect = 9.06e-248
Identity = 350/378 (92.59%), Postives = 358/378 (94.71%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN H SRLIEIDEGVGH IVLPGGI 
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVL DHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240
           KMGGKDVIYMKQQHSSTL   EPSEVQK LK+MADKRF+ET+ QYGMGSE +YNNDK E 
Sbjct: 181 KMGGKDVIYMKQQHSSTL---EPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNNDKFES 240

Query: 241 REQHLRFGDASSAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPIT 300
           REQHL+F DASSAIS LK DIV I KRRGGSDRI+LPHHEWLQTVQLEPDVIS+SFIPIT
Sbjct: 241 REQHLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPIT 300

Query: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLA 360

Query: 361 SLQFSLMGSKLYVNTTPV 377
           SLQFSLMGSKLYVNTTPV
Sbjct: 361 SLQFSLMGSKLYVNTTPV 375

BLAST of Cucsat.G243 vs. ExPASy TrEMBL
Match: A0A5D3BT29 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G00720 PE=4 SV=1)

HSP 1 Score: 759 bits (1960), Expect = 7.64e-273
Identity = 374/377 (99.20%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGI+
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVP+SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240
           KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300
           REQHLRFGDAS+AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS
Sbjct: 241 REQHLRFGDASTAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPV 377
           LQFSLMGSKLYVNTTPV
Sbjct: 361 LQFSLMGSKLYVNTTPV 377

BLAST of Cucsat.G243 vs. ExPASy TrEMBL
Match: A0A1S3BTG2 (MACPF domain-containing protein At4g24290 OS=Cucumis melo OX=3656 GN=LOC103493321 PE=4 SV=1)

HSP 1 Score: 759 bits (1960), Expect = 7.64e-273
Identity = 374/377 (99.20%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGI+
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVP+SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240
           KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300
           REQHLRFGDAS+AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS
Sbjct: 241 REQHLRFGDASTAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPV 377
           LQFSLMGSKLYVNTTPV
Sbjct: 361 LQFSLMGSKLYVNTTPV 377

BLAST of Cucsat.G243 vs. ExPASy TrEMBL
Match: A0A0A0L5E1 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G110020 PE=4 SV=1)

HSP 1 Score: 711 bits (1835), Expect = 3.71e-254
Identity = 356/377 (94.43%), Postives = 356/377 (94.43%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240
           KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK   
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDK--- 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300
                             DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS
Sbjct: 241 ------------------DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 356

Query: 361 LQFSLMGSKLYVNTTPV 377
           LQFSLMGSKLYVNTTPV
Sbjct: 361 LQFSLMGSKLYVNTTPV 356

BLAST of Cucsat.G243 vs. ExPASy TrEMBL
Match: A0A6J1J8T9 (MACPF domain-containing protein At4g24290 OS=Cucurbita maxima OX=3661 GN=LOC111482322 PE=4 SV=1)

HSP 1 Score: 695 bits (1794), Expect = 1.25e-247
Identity = 348/378 (92.06%), Postives = 359/378 (94.97%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN HGSRLIEIDEGVGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVL DHVRKAVPSSWDPAALARFIETFGTHIIVG 
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240
           KMGGKDVIYMKQQHSSTL   EPSEVQK LK+MADKRF+ET+ QYGMGSE +YN++K E 
Sbjct: 181 KMGGKDVIYMKQQHSSTL---EPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFES 240

Query: 241 REQHLRFGDASSAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPIT 300
           REQHL+F DASSAIS LK DI+ I KRRGGSDRI+LPHHEWLQTVQLEPDVIS+SFIPIT
Sbjct: 241 REQHLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPIT 300

Query: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLA 360

Query: 361 SLQFSLMGSKLYVNTTPV 377
           SLQFSLMGSKLYVNTTPV
Sbjct: 361 SLQFSLMGSKLYVNTTPV 375

BLAST of Cucsat.G243 vs. ExPASy TrEMBL
Match: A0A6J1FQI8 (MACPF domain-containing protein At4g24290 OS=Cucurbita moschata OX=3662 GN=LOC111447540 PE=4 SV=1)

HSP 1 Score: 694 bits (1791), Expect = 3.58e-247
Identity = 349/378 (92.33%), Postives = 357/378 (94.44%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60
           MALKVHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN H SRLIEIDEGVGH IVLPGGI 
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVL DHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240
           KMGGKDVIYMKQQHSSTL   EPSEVQK LK+MADKRF+ET+ QYGM SE +YNNDK E 
Sbjct: 181 KMGGKDVIYMKQQHSSTL---EPSEVQKRLKDMADKRFTETMGQYGMSSEWIYNNDKFES 240

Query: 241 REQHLRFGDASSAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPIT 300
           REQHL+F DASSAIS LK DIV I KRRGGSDRI+LPHHEWLQTVQLEPDVIS+SFIPIT
Sbjct: 241 REQHLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPIT 300

Query: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLA 360

Query: 361 SLQFSLMGSKLYVNTTPV 377
           SLQFSLMGSKLYVNTTPV
Sbjct: 361 SLQFSLMGSKLYVNTTPV 375

BLAST of Cucsat.G243 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 557.0 bits (1434), Expect = 1.3e-158
Identity = 278/380 (73.16%), Postives = 319/380 (83.95%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGH-EIVLPGGI 60
           MAL++  SKAAE+AIGSIG GYD+ IDLRLKYCKG    SRL++I EG  + EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  LISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
            I NV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DAA TKNLAFDGVFI+LY+VAL+KSQ++L +HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIE 240
           +KMGGKDVIY KQQHSS LQ   P ++QK LKE+ADKRF E    +  GSER+  + K+E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQ---PEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVE 240

Query: 241 IREQHLRFGDASS--AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIP 300
            +EQ LRF D SS  + +  +D V +CKRRGG+D  +L H+EWLQTVQ+EPDVISMSFIP
Sbjct: 241 TKEQRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 300

Query: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHN 360
           ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ +
Sbjct: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQS 360

Query: 361 LASLQFSLMGSKLYVNTTPV 378
            ASLQFS  G KLYVNTTPV
Sbjct: 361 CASLQFSFFGPKLYVNTTPV 377

BLAST of Cucsat.G243 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 458.0 bits (1177), Expect = 8.0e-129
Identity = 234/338 (69.23%), Postives = 275/338 (81.36%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGH-EIVLPGGI 60
           MAL++  SKAAE+AIGSIG GYD+ IDLRLKYCKG    SRL++I EG  + EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  LISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
            I NV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DAA TKNLAFDGVFI+LY+VAL+KSQ++L +HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIE 240
           +KMGGKDVIY KQQHSS LQ   P ++QK LKE+ADKRF E    +  GSER+  + K+E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQ---PEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVE 240

Query: 241 IREQHLRFGDASS--AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIP 300
            +EQ LRF D SS  + +  +D V +CKRRGG+D  +L H+EWLQTVQ+EPDVISMSFIP
Sbjct: 241 TKEQRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 300

Query: 301 ITSLLNGVPGSGFLSHAINLYLRYKP--PIEELHQFLE 334
           ITSLLNGVPGSGFLSHAINLYLR KP   +  +H FLE
Sbjct: 301 ITSLLNGVPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335

BLAST of Cucsat.G243 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 410.2 bits (1053), Expect = 1.9e-114
Identity = 209/377 (55.44%), Postives = 266/377 (70.56%), Query Frame = 0

Query: 10  AAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSIK 69
           AAE A+  IG GYD+  D+R   CK    GSRL+EID     +++ PGGI+++NV  SIK
Sbjct: 14  AAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK 73

Query: 70  CDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAF 129
           CDKGERTR RSD+LSF QMSE+FNQ+M L+GKIPSG FN MF FS CW KDA++ K LA+
Sbjct: 74  CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAY 133

Query: 130 DGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIY 189
           DG FI+LY+V + + Q+ L D V++ VPSSWD AALA FIE +GTH++VG+ MGGKDVI+
Sbjct: 134 DGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIH 193

Query: 190 MKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEIREQHLRFG- 249
           +KQ   S     EP E+QK LK   D+RF     +       +Y+    E  E  L++G 
Sbjct: 194 VKQMRKSN---HEPEEIQKMLKHWGDERFCVDPVE-SKSPASVYSGKPKE--ENLLQWGL 253

Query: 250 -----DASSAI---SPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 309
                  SSA+   +  ++I+ +C RRGG D +   H  WL TV   P+VISM F+PITS
Sbjct: 254 QPFGTSVSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITS 313

Query: 310 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 369
           LL+G+PG+GFLSHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R + +  S
Sbjct: 314 LLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPS 373

Query: 370 LQFSLMGSKLYVNTTPV 378
           LQFSLMG KLYVNT+ V
Sbjct: 374 LQFSLMGPKLYVNTSKV 383

BLAST of Cucsat.G243 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 318.5 bits (815), Expect = 7.6e-87
Identity = 173/383 (45.17%), Postives = 240/383 (62.66%), Query Frame = 0

Query: 12  EIAIGSIGRGYDITIDLRLKYCK---GNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSI 71
           E A+ S+G+G+D+T D RLKYCK   G+    RL+ +D+    E+ +PG  +  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEF-SGCWQKDAANTKNL 131
            CDKGERTRFRSD+L F +MSE FNQ  ++TGKIPSG+FN  F F SG W  DAAN K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 AFDGVFITLYTVALEK-SQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKD 191
             D   +TL+ + +   +++ L D VR AVPSSWDP  LARFIE +GTH+I G+ +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSET--IAQYGMGSERLYNNDKIEIREQH 251
           V+ ++Q  SS L   +   ++  L ++ D+ F+ +  ++   +     +++ + +  E  
Sbjct: 190 VVVVRQDKSSDL---DNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAF 249

Query: 252 LRFGDASS------AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPI 311
             F D  +      +I+    I  IC +RGG  R +  H EWL TV  +PD I+ +FIPI
Sbjct: 250 NVFDDKQTVAFNNFSINSQNGITVICAKRGGDGR-AKSHSEWLITVPDKPDAINFNFIPI 309

Query: 312 TSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNL 371
           TSLL  VPGSG LSHA++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      N+
Sbjct: 310 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFG---AAPNM 369

Query: 372 AS----LQFSLMGSKLYVNTTPV 378
           AS    L  + MG KLYVNTTPV
Sbjct: 370 ASAYPALHINFMGPKLYVNTTPV 385

BLAST of Cucsat.G243 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.8e-83
Identity = 167/364 (45.88%), Postives = 222/364 (60.99%), Query Frame = 0

Query: 14  AIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGILISNVPKSIKCDKG 73
           AI ++GRG+D+T D+RL YCKG   GSRL+ I+EG   ++ L  G L+ NVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKG-APGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAFDGVF 133
                R  V SF +M+E+FN    + G IP G FN MF ++G WQ DAA+TK+LA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 ITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIYMKQQ 193
           I LY V L K  +VL + +R+AVPSSWDPA+LA FIE +GTHI+  + +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEIREQHLRFGDASSA 253
            SS   P   SE++  + +M   RF E  +Q   G                L++ D    
Sbjct: 204 QSS---PLPVSEIENYVNDMIKHRFHEAESQSITGP---------------LKYKD---- 263

Query: 254 ISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSH 313
               KDI  I +RRGG D +   H  W +TV   PD+I+M+F PI SLL GVPG   L+ 
Sbjct: 264 ----KDITVIFRRRGGDD-LEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTR 323

Query: 314 AINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVN 373
           AI LYL YKPPIE+L  FL++Q+ R WAP  S L    QRK+   +SLQFSLMG KL+++
Sbjct: 324 AIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFIS 355

Query: 374 TTPV 378
              V
Sbjct: 384 ADQV 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9STW51.8e-15773.16MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4... [more]
Q9SGN62.7e-11355.44MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=... [more]
Q8L6121.1e-8545.17MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Q9C7N22.5e-8245.88MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=... [more]
Match NameE-valueIdentityDescription
XP_004133715.14.75e-274100.00MACPF domain-containing protein At4g24290 [Cucumis sativus] >XP_011650569.1 MACP... [more]
XP_008452249.11.58e-27299.20PREDICTED: MACPF domain-containing protein At4g24290 [Cucumis melo] >KAA0060601.... [more]
XP_038904757.12.47e-26998.14MACPF domain-containing protein At4g24290 [Benincasa hispida] >XP_038904758.1 MA... [more]
XP_023540812.11.57e-24892.59MACPF domain-containing protein At4g24290 [Cucurbita pepo subsp. pepo][more]
KAG7031360.19.06e-24892.59MACPF domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A5D3BT297.64e-27399.20MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BTG27.64e-27399.20MACPF domain-containing protein At4g24290 OS=Cucumis melo OX=3656 GN=LOC10349332... [more]
A0A0A0L5E13.71e-25494.43MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G110020 PE=4 ... [more]
A0A6J1J8T91.25e-24792.06MACPF domain-containing protein At4g24290 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FQI83.58e-24792.33MACPF domain-containing protein At4g24290 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT4G24290.21.3e-15873.16MAC/Perforin domain-containing protein [more]
AT4G24290.18.0e-12969.23MAC/Perforin domain-containing protein [more]
AT1G28380.11.9e-11455.44MAC/Perforin domain-containing protein [more]
AT1G14780.17.6e-8745.17MAC/Perforin domain-containing protein [more]
AT1G29690.11.8e-8345.88MAC/Perforin domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020864Membrane attack complex component/perforin (MACPF) domainSMARTSM00457MACPF_8coord: 124..320
e-value: 6.4E-25
score: 98.8
IPR020864Membrane attack complex component/perforin (MACPF) domainPFAMPF01823MACPFcoord: 106..318
e-value: 8.5E-34
score: 117.5
IPR020864Membrane attack complex component/perforin (MACPF) domainPROSITEPS51412MACPF_2coord: 1..332
score: 20.285645
NoneNo IPR availablePANTHERPTHR33199:SF16MEMBRANE ATTACK COMPLEX COMPONENT/PERFORIN (MACPF) DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 1..599
IPR044663MACPF domain-containing protein CAD1/NSL1-likePANTHERPTHR33199MACPF DOMAIN-CONTAINING PROTEIN CAD1coord: 1..599

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G243.T5Cucsat.G243.T5mRNA
Cucsat.G243.T1Cucsat.G243.T1mRNA
Cucsat.G243.T9Cucsat.G243.T9mRNA
Cucsat.G243.T2Cucsat.G243.T2mRNA
Cucsat.G243.T15Cucsat.G243.T15mRNA
Cucsat.G243.T14Cucsat.G243.T14mRNA
Cucsat.G243.T6Cucsat.G243.T6mRNA
Cucsat.G243.T10Cucsat.G243.T10mRNA
Cucsat.G243.T16Cucsat.G243.T16mRNA
Cucsat.G243.T19Cucsat.G243.T19mRNA
Cucsat.G243.T18Cucsat.G243.T18mRNA
Cucsat.G243.T20Cucsat.G243.T20mRNA
Cucsat.G243.T21Cucsat.G243.T21mRNA
Cucsat.G243.T4Cucsat.G243.T4mRNA
Cucsat.G243.T13Cucsat.G243.T13mRNA
Cucsat.G243.T12Cucsat.G243.T12mRNA
Cucsat.G243.T11Cucsat.G243.T11mRNA
Cucsat.G243.T17Cucsat.G243.T17mRNA
Cucsat.G243.T8Cucsat.G243.T8mRNA
Cucsat.G243.T7Cucsat.G243.T7mRNA
Cucsat.G243.T3Cucsat.G243.T3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0012501 programmed cell death
biological_process GO:2000031 regulation of salicylic acid mediated signaling pathway