Cucsat.G21212 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G21212
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	gibberellin 20 oxidase 1-like
Location	ctg910: 3612881 .. 3614334 (-)
RNA-Seq Expression	Cucsat.G21212
Synteny	Cucsat.G21212

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GACACACTACACACACTCCATGTAATGTTGTCACTGCCCAAACAAAAATAAAATAAAATAACTTAATTTATTTAAAGAAGAAGAAATTAAATTTGCCAATTGGGAGCTAGCTAGAAGACCCTTTTGGCAATTTTTAACATGATGAAGCCCCTTGTTTTTGTAAAATCAAAACTTTTATTTTTCTCAACATGTTTGCTCACACATAAATTCAGAACCTCCCCATGGCTTTTCTTTTGTCACCCTCTTTTCTAATTTCTTTTTCTCTCTAATAACCATGCATGCATGTAGTAACATCATCCATGGCTAAAGAGGCCGCCAATAACGACGGCGTTCCATTAGTGTTCGATGCATCGGTTCTACGACACCAACATAAGATTCCAAAGCAATTCATATGGCCAGATGAGGAGAAGCCAAGCGAGGCGGGGTGTCCAGAGATGGAGGTCCCATTAATCGACTTGTCCGGATTTTTATCCGGAGATAAAGATTCAGTTCGAGAAGCCGTCCGACACGTCGGTGAGGCTTGTGAGAAACATGGATTTTTTCTTGTGGTGAACCATGGAGTTGACTTAAAACTCATAGCAGATGCTCACAAATACATGAATGAATTCTTTGAATTGCCGCTTTGTGAAAAACAAAGGGCTCAAAGAAAAGTTGGCGAACATTGTGGTTATGCCAGTAGTTTCACTGGTCGATTCTCTTCTAAGCTACCTTGGAAAGAAACTCTTTCGTTTCGATTCTCTGCTGACGAATCCTTGGATGACCATGTTTTTCATTACCTTCGCACCAAACTCGGTGACCGATTTGCCAACTTTGGGTACTTCTTCCTTTCTCTAACCGTTCTCTTTGGAATGACTTTTTAATATAATATTTTCCATTTGGTTATACAGTAATTTTTGTTTATTTCAAACGTATGTTGTATAATATATGTAGTAATAATATTGTTCTGGTTTAAACAACAGTAGGGTTTATCAAGAGTACTGTGAAGCAATGAGTGGACTATCTTTGGGGATAATGGAGCTATTGGGGAAAAGCCTTGGAGTAGAAGGAGAGTACTTCAAGAACTTCTTCAAAGACGATGATTCAATAATGAGATTGAATTTTTATCCACCATGTCAAAAGCCACATCTCACCTTAGGGACAGGCCCTCATTGTGACCCAACTTCTTTGACCATTCTTCACCAAGACCAAGTTGGTGGCTTACAAGTTTTTGTAGACAACCAATGGAGACTCATTGCCCCTAATTTTGATGCTTTTGTTGTCAATATTGGTGACACTTTCATGGTAATCAAACAAACCACCCCCATCATCATCAAAATATACTCTTAACTTTTTTCTTTTTCTTTTCCAATTTTACCTTTAAAAAATCTACTCTCTTAACTTACCTAAGTTTAAAACGATTAAAATCACTCTCCACGATGCTTTTTCAAATTGATGATATAGAATTAACTTCTAAAAATATGATAGGTTTAAAAGTAATTTTTAGTTAACAATTTCAAAACTGAATTGTCAATTAATCACTTCTAAAAGTTTGACATCTTTGTTTTTTTAAAAAAACGTATTCATAAGAATAATATTAACAATTAGAGAACCTATACACACTTTGACTTGATAAAAGATAAAGTCGAGTTTTCTAATCGTAGGACCTACAAACTTTCTTAAACCAACTCTGATAACATATGCCTTGGTTCGTACGTATATATACACATACTAATGATGGTTTTGTTGTTTTTGTGGGGAAAAAAAGGCACTATCAAATGGAAGATACAAGAGTTGCTTGCATAGAGCAGTGGTGAACAGCAAGAGAACAAGGAAATCTTTAGCATTTTTTTTATGTCCAAGAAATGATAAAGTGGTAAAGCCACCAATGGAGTTGGTGGACAATGAGAACCCAAGAATATATCCAGATTTTACATGGTCCATGCTTCTCCAATTCACACAGAATAATTATAGAGCTGATATGAAAACACTTGAAGTTTTTTCAGCTTCTCTTCAACAACAACAATCTTAATTATTAATTATTTATAATATATTTATTCTTCCTCTCTTCTTTCCTTAGTATTATTGCCTCTTTCTCTATGCTAAAAGAGTTTTTTCTTGTGGTGATGAGGAAAATAGAAGTGACTCTTTTGTTTCTTTTTCTTTTTTAATTTTACTCTCTCAATGGAATGACAAGGGAAAAGTTGAGGGAAAAGCCAGTTCCTTTTCTTTTGTTTTTGTCTTTTTCTTCTTCTTATTTTATATATATATATATATATGTCTTAATGTGTTTTTGGATCTTAGTTAGTAACTTTCTTAGCTTCTAGTGAAATGAACCTCTAGTTTTAAAAAAGATAAATTGCTAATAATGTTTTGTGTGTGTGTAGTTTTTAGTCCCTAATAGTTTCTG

Coding sequence (CDS)

ATGCATGTAGTAACATCATCCATGGCTAAAGAGGCCGCCAATAACGACGGCGTTCCATTAGTGTTCGATGCATCGGTTCTACGACACCAACATAAGATTCCAAAGCAATTCATATGGCCAGATGAGGAGAAGCCAAGCGAGGCGGGGTGTCCAGAGATGGAGGTCCCATTAATCGACTTGTCCGGATTTTTATCCGGAGATAAAGATTCAGTTCGAGAAGCCGTCCGACACGTCGGTGAGGCTTGTGAGAAACATGGATTTTTTCTTGTGGTGAACCATGGAGTTGACTTAAAACTCATAGCAGATGCTCACAAATACATGAATGAATTCTTTGAATTGCCGCTTTGTGAAAAACAAAGGGCTCAAAGAAAAGTTGGCGAACATTGTGGTTATGCCAGTAGTTTCACTGGTCGATTCTCTTCTAAGCTACCTTGGAAAGAAACTCTTTCGTTTCGATTCTCTGCTGACGAATCCTTGGATGACCATGTTTTTCATTACCTTCGCACCAAACTCGGTGACCGATTTGCCAACTTTGGTAGGGTTTATCAAGAGTACTGTGAAGCAATGAGTGGACTATCTTTGGGGATAATGGAGCTATTGGGGAAAAGCCTTGGAGTAGAAGGAGAGTACTTCAAGAACTTCTTCAAAGACGATGATTCAATAATGAGATTGAATTTTTATCCACCATGTCAAAAGCCACATCTCACCTTAGGGACAGGCCCTCATTGTGACCCAACTTCTTTGACCATTCTTCACCAAGACCAAGTTGGTGGCTTACAAGTTTTTGTAGACAACCAATGGAGACTCATTGCCCCTAATTTTGATGCTTTTGTTGTCAATATTGGTGACACTTTCATGGCACTATCAAATGGAAGATACAAGAGTTGCTTGCATAGAGCAGTGGTGAACAGCAAGAGAACAAGGAAATCTTTAGCATTTTTTTTATGTCCAAGAAATGATAAAGTGGTAAAGCCACCAATGGAGTTGGTGGACAATGAGAACCCAAGAATATATCCAGATTTTACATGGTCCATGCTTCTCCAATTCACACAGAATAATTATAGAGCTGATATGAAAACACTTGAAGTTTTTTCAGCTTCTCTTCAACAACAACAATCTTAA

Protein sequence

MHVVTSSMAKEAANNDGVPLVFDASVLRHQHKIPKQFIWPDEEKPSEAGCPEMEVPLIDLSGFLSGDKDSVREAVRHVGEACEKHGFFLVVNHGVDLKLIADAHKYMNEFFELPLCEKQRAQRKVGEHCGYASSFTGRFSSKLPWKETLSFRFSADESLDDHVFHYLRTKLGDRFANFGRVYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQKPHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGRYKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQNNYRADMKTLEVFSASLQQQQS

Homology

[Close]

BLAST of Cucsat.G21212 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 303.1 bits (775), Expect = 2.3e-81
Identity = 145/191 (75.92%), Postives = 161/191 (84.29%), Query Frame = 0


Query: 3   FANFG-VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQKPH 62
           F  FG VYQEYCEAMS LSL IMELLG SLGV+ +YF+ FF+++DSIMRLN+YPPC KP 
Sbjct: 180 FQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPD 239

Query: 63  LTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGRYK 122
           LTLGTGPHCDPTSLTILHQD V GLQVFV+NQWR I PN  AFVVNIGDTFMALSN RYK
Sbjct: 240 LTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYK 299

Query: 123 SCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQNNY 182
           SCLHRAVVNS+  RKSLAFFLCP+ D+VV PP EL+D+   R YPDFTWSM L+FTQ +Y
Sbjct: 300 SCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLDSITSRRYPDFTWSMFLEFTQKHY 359

Query: 183 RADMKTLEVFS 193
           RADM TL+ FS
Sbjct: 360 RADMNTLQAFS 370

[Close]

BLAST of Cucsat.G21212 vs. ExPASy Swiss-Prot
Match: Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 1.2e-79
Identity = 145/203 (71.43%), Postives = 166/203 (81.77%), Query Frame = 0


Query: 1   DRFANFG-VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQK 60
           D + +FG VYQEY EAM+ LSL IMELLG SLGVE  YFK FF+D DSI RLN+YP C++
Sbjct: 177 DGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQ 236

Query: 61  PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 120
           P L LGTGPHCDPTSLTILHQDQVGGLQVFVDN+W+ I PN  AFVVNIGDTFMAL+NGR
Sbjct: 237 PELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGR 296

Query: 121 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVD--NENPRIYPDFTWSMLLQFT 180
           YKSCLHRAVVNS+R RK+ AFFLCP+ +KVVKPP ELV+      R YPDFTWSM L+FT
Sbjct: 297 YKSCLHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFT 356

Query: 181 QNNYRADMKTLEVFSASLQQQQS 201
           Q +YRADM TL+ FS  L+ ++S
Sbjct: 357 QKHYRADMNTLDEFSIWLKNRRS 379

[Close]

BLAST of Cucsat.G21212 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 1.8e-78
Identity = 138/191 (72.25%), Postives = 158/191 (82.72%), Query Frame = 0


Query: 3   FANFG-VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQKPH 62
           F  FG VYQ+YCEAMS LSL IMELLG SLGV  +YF+ FF+++DSIMRLN YPPCQ P 
Sbjct: 178 FEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPD 237

Query: 63  LTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGRYK 122
           LTLGTGPHCDP+SLTILHQD V GLQVFVDNQW+ I PN  AFVVNIGDTFMALSNG +K
Sbjct: 238 LTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNGIFK 297

Query: 123 SCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQNNY 182
           SCLHRAVVN +  RKS+AFFLCP+ DKVVKPP ++++    R YPDFTWSM L+FTQ +Y
Sbjct: 298 SCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQKHY 357

Query: 183 RADMKTLEVFS 193
           RAD+ TL+ FS
Sbjct: 358 RADVNTLDSFS 368

[Close]

BLAST of Cucsat.G21212 vs. ExPASy Swiss-Prot
Match: O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 4.0e-78
Identity = 141/192 (73.44%), Postives = 155/192 (80.73%), Query Frame = 0


Query: 8   VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQKPHLTLGTG 67
           VY  YC  MS LSL IME+LG+SLGV   +++ FF+ +DSIMRLN+YPPCQ+P  TLGTG
Sbjct: 163 VYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPMETLGTG 222

Query: 68  PHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGRYKSCLHRA 127
           PHCDPTSLTILHQD VGGLQV  + +WR I P  DAFVVNIGDTFMALSNGRYKSCLHRA
Sbjct: 223 PHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRA 282

Query: 128 VVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQNNYRADMKT 187
           VVNSK  RKSLAFFLCP  DKVV PP  LVD  NPR YPDFTW  LL FTQ +YRADMKT
Sbjct: 283 VVNSKVPRKSLAFFLCPEMDKVVAPPGTLVDAANPRAYPDFTWRSLLDFTQKHYRADMKT 342

Query: 188 LEVFSASLQQQQ 200
           LEVFS+ + QQQ
Sbjct: 343 LEVFSSWIVQQQ 354

[Close]

BLAST of Cucsat.G21212 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 8.9e-78
Identity = 140/192 (72.92%), Postives = 155/192 (80.73%), Query Frame = 0


Query: 8   VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQKPHLTLGTG 67
           VY  YC  MS LSL IME+LG+SLGV   +++ FF+ +DSIMRLN+YPPCQ+P  TLGTG
Sbjct: 163 VYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTG 222

Query: 68  PHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGRYKSCLHRA 127
           PHCDPTSLTILHQD VGGLQV  + +WR I P  DAFVVNIGDTFMALSNGRYKSCLHRA
Sbjct: 223 PHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRA 282

Query: 128 VVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQNNYRADMKT 187
           VVNS+  RKSLAFFLCP  DKVV PP  LVD  NPR YPDFTW  LL FTQ +YRADMKT
Sbjct: 283 VVNSRVPRKSLAFFLCPEMDKVVAPPGTLVDASNPRAYPDFTWRSLLDFTQKHYRADMKT 342

Query: 188 LEVFSASLQQQQ 200
           LEVFS+ + QQQ
Sbjct: 343 LEVFSSWIVQQQ 354

[Close]

BLAST of Cucsat.G21212 vs. NCBI nr
Match: XP_004143646.1 (gibberellin 20 oxidase 1-D [Cucumis sativus] >KGN50396.1 hypothetical protein Csa_000340 [Cucumis sativus] >CBX88043.1 gibberellin 20-oxidase, partial [Cucumis sativus])

HSP 1 Score: 410 bits (1055), Expect = 4.49e-142
Identity = 199/201 (99.00%), Postives = 200/201 (99.50%), Query Frame = 0


Query: 1   DRFANFG-VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQK 60
           DRFANFG VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKD+DSIMRLNFYPPCQK
Sbjct: 173 DRFANFGRVYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDNDSIMRLNFYPPCQK 232

Query: 61  PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 120
           PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR
Sbjct: 233 PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 292

Query: 121 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQN 180
           YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQN
Sbjct: 293 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQN 352

Query: 181 NYRADMKTLEVFSASLQQQQS 200
           NYRADMKTLEVFSASLQQQQS
Sbjct: 353 NYRADMKTLEVFSASLQQQQS 373

[Close]

BLAST of Cucsat.G21212 vs. NCBI nr
Match: XP_008467255.1 (PREDICTED: gibberellin 20 oxidase 1-like [Cucumis melo] >KAA0055149.1 gibberellin 20 oxidase 1-like [Cucumis melo var. makuwa] >TYK00313.1 gibberellin 20 oxidase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 397 bits (1021), Expect = 7.13e-137
Identity = 193/199 (96.98%), Postives = 194/199 (97.49%), Query Frame = 0


Query: 1   DRFANFG-VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQK 60
           DRF NFG VYQEYCEAMSGLSLGIMELLGKSLGV GEYFKNFFKD+DSIMRLNFYPPCQK
Sbjct: 173 DRFVNFGRVYQEYCEAMSGLSLGIMELLGKSLGVGGEYFKNFFKDNDSIMRLNFYPPCQK 232

Query: 61  PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 120
           P LTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR
Sbjct: 233 PDLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 292

Query: 121 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQN 180
           YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPP ELVDNENPRIYPDFTWSMLLQFTQN
Sbjct: 293 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPRELVDNENPRIYPDFTWSMLLQFTQN 352

Query: 181 NYRADMKTLEVFSASLQQQ 198
           NYRADMKTLEVFSASLQQQ
Sbjct: 353 NYRADMKTLEVFSASLQQQ 371

[Close]

BLAST of Cucsat.G21212 vs. NCBI nr
Match: XP_038875530.1 (gibberellin 20 oxidase 1-like [Benincasa hispida])

HSP 1 Score: 395 bits (1015), Expect = 4.59e-136
Identity = 190/199 (95.48%), Postives = 195/199 (97.99%), Query Frame = 0


Query: 1   DRFANFG-VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQK 60
           D+FANFG VYQEYCEAMSGLSLGIMELLGKSLGVEG+YFKNFFKD+DSIMRLNFYPPCQK
Sbjct: 169 DQFANFGRVYQEYCEAMSGLSLGIMELLGKSLGVEGDYFKNFFKDNDSIMRLNFYPPCQK 228

Query: 61  PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 120
           PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR
Sbjct: 229 PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 288

Query: 121 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQN 180
           YKSCLHRAVVNS+ TRKSLAFFLCPRNDKVVKPP ELVD +NPRIYPDFTWSMLLQFTQN
Sbjct: 289 YKSCLHRAVVNSETTRKSLAFFLCPRNDKVVKPPKELVDTQNPRIYPDFTWSMLLQFTQN 348

Query: 181 NYRADMKTLEVFSASLQQQ 198
           NYRADMKTLEVFSASLQQQ
Sbjct: 349 NYRADMKTLEVFSASLQQQ 367

[Close]

BLAST of Cucsat.G21212 vs. NCBI nr
Match: KAG6579665.1 (Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 379 bits (974), Expect = 3.47e-130
Identity = 178/197 (90.36%), Postives = 189/197 (95.94%), Query Frame = 0


Query: 1   DRFANFGVYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQKP 60
           D+F +FGVYQEYCEAMSGLSLGIMELLGKSLGVEG+YFKNFFKD+DSIMRLNFYPPCQKP
Sbjct: 147 DQFVDFGVYQEYCEAMSGLSLGIMELLGKSLGVEGDYFKNFFKDNDSIMRLNFYPPCQKP 206

Query: 61  HLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGRY 120
           HLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFV+NIGDTFMALSNGRY
Sbjct: 207 HLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVINIGDTFMALSNGRY 266

Query: 121 KSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQNN 180
           KSCLHRAVVN++ TRKSLAFFLCPRNDKVV+PP ELVD +NPRIYPDFTWSML  FT NN
Sbjct: 267 KSCLHRAVVNNETTRKSLAFFLCPRNDKVVRPPRELVDTQNPRIYPDFTWSMLHHFTLNN 326

Query: 181 YRADMKTLEVFSASLQQ 197
           YRADMKTL+VFSA L+Q
Sbjct: 327 YRADMKTLDVFSAWLRQ 343

[Close]

BLAST of Cucsat.G21212 vs. NCBI nr
Match: KAG6579667.1 (Gibberellin 20 oxidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 379 bits (974), Expect = 5.99e-130
Identity = 178/197 (90.36%), Postives = 189/197 (95.94%), Query Frame = 0


Query: 1   DRFANFGVYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQKP 60
           D+F +FGVYQEYCEAMSGLSLGIMELLGKSLGVEG+YFKNFFKD+DSIMRLNFYPPCQKP
Sbjct: 163 DQFVDFGVYQEYCEAMSGLSLGIMELLGKSLGVEGDYFKNFFKDNDSIMRLNFYPPCQKP 222

Query: 61  HLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGRY 120
           HLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFV+NIGDTFMALSNGRY
Sbjct: 223 HLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVINIGDTFMALSNGRY 282

Query: 121 KSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQNN 180
           KSCLHRAVVN++ TRKSLAFFLCPRNDKVV+PP ELVD +NPRIYPDFTWSML  FT NN
Sbjct: 283 KSCLHRAVVNNETTRKSLAFFLCPRNDKVVRPPRELVDTQNPRIYPDFTWSMLHHFTLNN 342

Query: 181 YRADMKTLEVFSASLQQ 197
           YRADMKTL+VFSA L+Q
Sbjct: 343 YRADMKTLDVFSAWLRQ 359

[Close]

BLAST of Cucsat.G21212 vs. ExPASy TrEMBL
Match: K7ZU06 (Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=ga20ox1 PE=2 SV=1)

HSP 1 Score: 410 bits (1055), Expect = 2.17e-142
Identity = 199/201 (99.00%), Postives = 200/201 (99.50%), Query Frame = 0


Query: 1   DRFANFG-VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQK 60
           DRFANFG VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKD+DSIMRLNFYPPCQK
Sbjct: 173 DRFANFGRVYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDNDSIMRLNFYPPCQK 232

Query: 61  PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 120
           PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR
Sbjct: 233 PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 292

Query: 121 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQN 180
           YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQN
Sbjct: 293 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQN 352

Query: 181 NYRADMKTLEVFSASLQQQQS 200
           NYRADMKTLEVFSASLQQQQS
Sbjct: 353 NYRADMKTLEVFSASLQQQQS 373

[Close]

BLAST of Cucsat.G21212 vs. ExPASy TrEMBL
Match: A0A5D3BKN1 (Gibberellin 20 oxidase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00600 PE=3 SV=1)

HSP 1 Score: 397 bits (1021), Expect = 3.45e-137
Identity = 193/199 (96.98%), Postives = 194/199 (97.49%), Query Frame = 0


Query: 1   DRFANFG-VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQK 60
           DRF NFG VYQEYCEAMSGLSLGIMELLGKSLGV GEYFKNFFKD+DSIMRLNFYPPCQK
Sbjct: 173 DRFVNFGRVYQEYCEAMSGLSLGIMELLGKSLGVGGEYFKNFFKDNDSIMRLNFYPPCQK 232

Query: 61  PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 120
           P LTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR
Sbjct: 233 PDLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 292

Query: 121 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQN 180
           YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPP ELVDNENPRIYPDFTWSMLLQFTQN
Sbjct: 293 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPRELVDNENPRIYPDFTWSMLLQFTQN 352

Query: 181 NYRADMKTLEVFSASLQQQ 198
           NYRADMKTLEVFSASLQQQ
Sbjct: 353 NYRADMKTLEVFSASLQQQ 371

[Close]

BLAST of Cucsat.G21212 vs. ExPASy TrEMBL
Match: A0A1S3CT51 (gibberellin 20 oxidase 1-like OS=Cucumis melo OX=3656 GN=LOC103504653 PE=3 SV=1)

HSP 1 Score: 397 bits (1021), Expect = 3.45e-137
Identity = 193/199 (96.98%), Postives = 194/199 (97.49%), Query Frame = 0


Query: 1   DRFANFG-VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQK 60
           DRF NFG VYQEYCEAMSGLSLGIMELLGKSLGV GEYFKNFFKD+DSIMRLNFYPPCQK
Sbjct: 173 DRFVNFGRVYQEYCEAMSGLSLGIMELLGKSLGVGGEYFKNFFKDNDSIMRLNFYPPCQK 232

Query: 61  PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 120
           P LTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR
Sbjct: 233 PDLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 292

Query: 121 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQN 180
           YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPP ELVDNENPRIYPDFTWSMLLQFTQN
Sbjct: 293 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPRELVDNENPRIYPDFTWSMLLQFTQN 352

Query: 181 NYRADMKTLEVFSASLQQQ 198
           NYRADMKTLEVFSASLQQQ
Sbjct: 353 NYRADMKTLEVFSASLQQQ 371

[Close]

BLAST of Cucsat.G21212 vs. ExPASy TrEMBL
Match: A0A6J1H0C4 (gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459279 PE=3 SV=1)

HSP 1 Score: 377 bits (968), Expect = 4.35e-129
Identity = 181/200 (90.50%), Postives = 190/200 (95.00%), Query Frame = 0


Query: 1   DRFANFG-VYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQK 60
           D+FA FG VYQ+YCEAMSGLSLGIMELLGKSLGVE +YFKNFFKD+DSIMRLNFYPPCQK
Sbjct: 169 DQFAKFGRVYQDYCEAMSGLSLGIMELLGKSLGVEEQYFKNFFKDNDSIMRLNFYPPCQK 228

Query: 61  PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGR 120
           PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLI PNFDAFVVNIGDTFMALSNGR
Sbjct: 229 PHLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLITPNFDAFVVNIGDTFMALSNGR 288

Query: 121 YKSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQN 180
           YKSCLHRAVVNS+RTRKSLAFFLCPRNDKVV+PP ELVD +NPR YPDFTWSMLL+FTQ 
Sbjct: 289 YKSCLHRAVVNSERTRKSLAFFLCPRNDKVVRPPRELVDTQNPRRYPDFTWSMLLRFTQT 348

Query: 181 NYRADMKTLEVFSASLQQQQ 199
           +YRADMKTLE FSA LQQQQ
Sbjct: 349 HYRADMKTLEAFSAWLQQQQ 368

[Close]

BLAST of Cucsat.G21212 vs. ExPASy TrEMBL
Match: A0A6J1EM68 (gibberellin 20 oxidase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435674 PE=3 SV=1)

HSP 1 Score: 376 bits (966), Expect = 4.77e-129
Identity = 177/197 (89.85%), Postives = 188/197 (95.43%), Query Frame = 0


Query: 1   DRFANFGVYQEYCEAMSGLSLGIMELLGKSLGVEGEYFKNFFKDDDSIMRLNFYPPCQKP 60
           D+F +F VYQEYCEAMSGLSLGIMELLGKSLGVEG+YFKNFFKD+DSIMRLNFYPPCQKP
Sbjct: 163 DQFVDFRVYQEYCEAMSGLSLGIMELLGKSLGVEGDYFKNFFKDNDSIMRLNFYPPCQKP 222

Query: 61  HLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVVNIGDTFMALSNGRY 120
           HLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFV+NIGDTFMALSNGRY
Sbjct: 223 HLTLGTGPHCDPTSLTILHQDQVGGLQVFVDNQWRLIAPNFDAFVINIGDTFMALSNGRY 282

Query: 121 KSCLHRAVVNSKRTRKSLAFFLCPRNDKVVKPPMELVDNENPRIYPDFTWSMLLQFTQNN 180
           KSCLHRAVVN++ TRKSLAFFLCPRNDKVV+PP ELVD +NPRIYPDFTWSML  FT NN
Sbjct: 283 KSCLHRAVVNNETTRKSLAFFLCPRNDKVVRPPRELVDTQNPRIYPDFTWSMLHHFTLNN 342

Query: 181 YRADMKTLEVFSASLQQ 197
           YRADMKTL+VFSA L+Q
Sbjct: 343 YRADMKTLDVFSAWLRQ 359

The following BLAST results are available for this feature:

BLAST of Cucsat.G21212 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39110	2.3e-81	75.92	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]
Q39112	1.2e-79	71.43	Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1	[more]
Q39111	1.8e-78	72.25	Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1	[more]
O04706	4.0e-78	73.44	Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1	[more]
O04707	8.9e-78	72.92	Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1	[more]

BLAST of Cucsat.G21212 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143646.1	4.49e-142	99.00	gibberellin 20 oxidase 1-D [Cucumis sativus] >KGN50396.1 hypothetical protein Cs...	[more]
XP_008467255.1	7.13e-137	96.98	PREDICTED: gibberellin 20 oxidase 1-like [Cucumis melo] >KAA0055149.1 gibberelli...	[more]
XP_038875530.1	4.59e-136	95.48	gibberellin 20 oxidase 1-like [Benincasa hispida]	[more]
KAG6579665.1	3.47e-130	90.36	Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6579667.1	5.99e-130	90.36	Gibberellin 20 oxidase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cucsat.G21212 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
K7ZU06	2.17e-142	99.00	Gibberellin 20-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=ga20ox1 PE=2 SV=...	[more]
A0A5D3BKN1	3.45e-137	96.98	Gibberellin 20 oxidase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3CT51	3.45e-137	96.98	gibberellin 20 oxidase 1-like OS=Cucumis melo OX=3656 GN=LOC103504653 PE=3 SV=1	[more]
A0A6J1H0C4	4.35e-129	90.50	gibberellin 20 oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459279 PE=3...	[more]
A0A6J1EM68	4.77e-129	89.85	gibberellin 20 oxidase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..161 e-value: 1.2E-58 score: 201.3
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 31..129 e-value: 6.5E-29 score: 100.4
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 1..179
None	No IPR available	PANTHER	PTHR47990:SF26	GIBBERELLIN 20-OXIDASE	coord: 1..179
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 1..169
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 29..129 score: 14.2339

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G21212.T1	Cucsat.G21212.T1	mRNA
Cucsat.G21212.T5	Cucsat.G21212.T5	mRNA
Cucsat.G21212.T2	Cucsat.G21212.T2	mRNA
Cucsat.G21212.T3	Cucsat.G21212.T3	mRNA
Cucsat.G21212.T4	Cucsat.G21212.T4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009908	flower development
biological_process	GO:0009686	gibberellin biosynthetic process
biological_process	GO:0009416	response to light stimulus
biological_process	GO:0009826	unidimensional cell growth
molecular_function	GO:0045544	gibberellin 20-oxidase activity
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding