Cucsat.G21085 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G21085
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Mannan endo-1,4-beta-mannosidase
Location	ctg910: 1408039 .. 1410032 (-)
RNA-Seq Expression	Cucsat.G21085
Synteny	Cucsat.G21085

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCATTTCCTATTCATCTCATTTCTCCAACCACTAAAATGGCTAAAGCTTTCCCTTTAATTTCTATATTATTTCTCTCTTTGTTGGTTCTTTCACAATCCTTACCTCTTTCCACAAGAGGAAGATGGATTGTAGACTCTAAAACAGGCCAACGTGTGAAGCTGAGTTGTGTCAATTTGGTATCTCATGCACAATCTATGGTGGCACAAGGTCTTGACAAACGACCATTGAAGGACTTAGCTAATGAGATTGTCAGTCGTGATTACAACTGTGTGCGCTTGACTTGGTCAGTCTACATGTTCACTCGTTACCCCTTCGAGACTATAGGTGATGTCCTAGACGGTCTCGACATCAACAAAGTGAAAAATGGAGTCAAAAAACACAACCCTCAGTTTTTGAACATGACAGTTACAAGTGCATTCAAAACTGTTGTTGATGGGTTGGGCAATGCCGGCCTCATGGTCATTTTGGACAATCACATAAGCCAACCCAGATGGTGTTGTTCTCTCCATGATGGAAATGGCTTCTTTGGAGACCGCAATTTCAAGCCTATTGAATGGCTTCGAGGCTTGGCCTATGTTGCTAGACATTTCAGTTGGCATCCTAAAGTGAGCTTTTTTTTTTTTCTTTTTTTAAATTACTTTTCTTTTTAAAAGTTAACATATATGTGTTGTTCTGTGTGCATAGGTGGTAGGAATGAGCCTAAGAAATGAACTTCGAGGGTCGAACAACGTCGGCGTGTGGCGGAAATATGTGAAACTAGGAAGTCACTTGATTCATAGAATAAATCCTAGACTTCTTGTAGTGATTTCCGGTCTAAACTACGATAACGATCTAAGTTACCTGAAGAAAAAGCCATTAGGGTACAACTTAAACAACAAAGTAGTGTTGGAGGCACATTTATACTCATTCAGTGGGGAACCTGAAAGCAAGTTTGTGAAGAAGCCATTAAACATAGCATGCAATCAAGTGATGGATAAATTCGAGAGAGAAGCTGGATTTGTGGTGGACATGAAGGATCCATATCCACTGTTTCTTAGTGAATTTGGGTACGATCTAAGGGGTGGTAACAAGGCTCAAAATAGGTTCATGAGTTGTTTTTTGGCTCGCATTATTGGCAAGGATATTGACTGGGCTTATTGGGCTTTCCAAGGATCCTATATGTATAGGCAAGGCCAACAAGATGTTGATGAAAGTTTTGGTATCATGGATAGCAGTTGGACTAAAGATCGAAGCCCTCGTCTCCAACAAATGCTTCAACTTGCCAAAAGAATCAACCAAGGTTACGTTTCTTAACTAACTCCAATCCTAACCCTAAACCTAAACTCTAACTTTTTTTTTTCTTTGTTGAACAGATCCAAACTCCAAAGGACCAATGTCCTACATAATGCTACATCCAGTGTCAGGGCAATGTGTCAAACTAGATGGCAAGGGAGGAATTGAACTAGGTGACTGTGAGACACCGACGTTGTGGGACCATACTGGAGATGGAAGTCCGATGAAGTTGTGGAACGGGCAGTGTTTGAAGTCAGCCGGAGATGGGAAGCCGCCTGTGGTGTCGGCCGAATGCTCTGGTGACGGAAGCAGTTGGACGGTGGCTTCTAAGGCAAAGCTTCAATTGTCCACCAAAAGTGGGGGAGAAAACATTTGCTTAGAGAAGGAATCTGATACTTCAATTGTTGTGAAGAAATGCATTTGCCTTAAAGATGAGTGGAATTGCTTCGATGATCCTCAGAGCCAGTGGTTCAAGCTTGTTCCAACAAATGTTGCTTAGTTTAGTAATTAGTTTTATTTAGGAAATTAGATTGTTAATTTAAAAGGGAATAATTGAGAATTAGTTTAAGATATTTAGTTAATAGTTTTTTTCTTCTAGTTTTTTAGGGATTTTGGCATGAAGCATATAGAGAGGTTAGAGTCGAAGGTTGTCTGATATGTTATTAGTTAATGTGAATATCTTATTACATAATTTTGTGAATAACAAATTTTCTCATCCTT

Coding sequence (CDS)

TCATTTCCTATTCATCTCATTTCTCCAACCACTAAAATGGCTAAAGCTTTCCCTTTAATTTCTATATTATTTCTCTCTTTGTTGGTTCTTTCACAATCCTTACCTCTTTCCACAAGAGGAAGATGGATTGTAGACTCTAAAACAGGCCAACGTGTGAAGCTGAGTTGTGTCAATTTGGTATCTCATGCACAATCTATGGTGGCACAAGGTCTTGACAAACGACCATTGAAGGACTTAGCTAATGAGATTGTCAGTCGTGATTACAACTGTGTGCGCTTGACTTGGTCAGTCTACATGTTCACTCGTTACCCCTTCGAGACTATAGGTGATGTCCTAGACGGTCTCGACATCAACAAAGTGAAAAATGGAGTCAAAAAACACAACCCTCAGTTTTTGAACATGACAGTTACAAGTGCATTCAAAACTGTTGTTGATGGGTTGGGCAATGCCGGCCTCATGGTCATTTTGGACAATCACATAAGCCAACCCAGATGGTGTTGTTCTCTCCATGATGGAAATGGCTTCTTTGGAGACCGCAATTTCAAGCCTATTGAATGGCTTCGAGGCTTGGCCTATGTTGCTAGACATTTCAGTTGGCATCCTAAAGTGGTAGGAATGAGCCTAAGAAATGAACTTCGAGGGTCGAACAACGTCGGCGTGTGGCGGAAATATGTGAAACTAGGAAGTCACTTGATTCATAGAATAAATCCTAGACTTCTTGTAGTGATTTCCGGTCTAAACTACGATAACGATCTAAGTTACCTGAAGAAAAAGCCATTAGGGTACAACTTAAACAACAAAGTAGTGTTGGAGGCACATTTATACTCATTCAGTGGGGAACCTGAAAGCAAGTTTGTGAAGAAGCCATTAAACATAGCATGCAATCAAGTGATGGATAAATTCGAGAGAGAAGCTGGATTTGTGGTGGACATGAAGGATCCATATCCACTGTTTCTTAGTGAATTTGGGTACGATCTAAGGGGTGGTAACAAGGCTCAAAATAGGTTCATGAGTTGTTTTTTGGCTCGCATTATTGGCAAGGATATTGACTGGGCTTATTGGGCTTTCCAAGGATCCTATATGTATAGGCAAGGCCAACAAGATGTTGATGAAAGTTTTGGTATCATGGATAGCAGTTGGACTAAAGATCGAAGCCCTCGTCTCCAACAAATGCTTCAACTTGCCAAAAGAATCAACCAAGATCCAAACTCCAAAGGACCAATGTCCTACATAATGCTACATCCAGTGTCAGGGCAATGTGTCAAACTAGATGGCAAGGGAGGAATTGAACTAGGTGACTGTGAGACACCGACGTTGTGGGACCATACTGGAGATGGAAGTCCGATGAAGTTGTGGAACGGGCAGTGTTTGAAGTCAGCCGGAGATGGGAAGCCGCCTGTGGTGTCGGCCGAATGCTCTGGTGACGGAAGCAGTTGGACGGTGGCTTCTAAGGCAAAGCTTCAATTGTCCACCAAAAGTGGGGGAGAAAACATTTGCTTAGAGAAGGAATCTGATACTTCAATTGTTGTGAAGAAATGCATTTGCCTTAAAGATGAGTGGAATTGCTTCGATGATCCTCAGAGCCAGTGGTTCAAGCTTGTTCCAACAAATGTTGCTTAG

Protein sequence

SFPIHLISPTTKMAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFLNMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAYVARHFSWHPKVVGMSLRNELRGSNNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDLSYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMKDPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDESFGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELGDCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTKSGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA

Homology

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BLAST of Cucsat.G21085 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.5e-108
Identity = 206/539 (38.22%), Postives = 319/539 (59.18%), Query Frame = 0


Query: 19  LISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLDKRPLKD 78
           L ++L L +   S SLPL TRGRWIVD  TG RVKL+CVN V H +  + +GL++ P+  
Sbjct: 14  LTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVAT 73

Query: 79  LANEIVSRDYNCVRLTWSVYMFTR--YPFETIGDVLDGLDINKVKNGVKKHNPQFLNMTV 138
           +A+ I S  +NCVRLT+S++M TR  Y   T+      L++ +  +G++ +NP+ L++  
Sbjct: 74  VAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGH 133

Query: 139 TSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAYVARH 198
            +A+  VV  L  AG+MVILDNH+S+P+WCC++ DGNGFFGDR F P  W+ GL  +A +
Sbjct: 134 VAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATY 193

Query: 199 FSWHPKVVGMSLRNELRGSNNVGV-WRKYVKLGSHLIHRINPRLLVVISGLNYDNDLSYL 258
           F+  P VV MSLRNELRG+ +  + W ++++ G+  +H+ NP++LV++SGL +D DLS+L
Sbjct: 194 FNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFL 253

Query: 259 KKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMKD-- 318
              P+      K+V E H YSF G P    +    N  C     +F+   GFV    +  
Sbjct: 254 PVLPVTLPFKEKIVYEGHWYSF-GVPWRTGLP---NDVCKNETGRFKSNVGFVTSSANAT 313

Query: 319 PYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQ---DVD 378
             PLF+SEFG D R  N   NR+++C LA +  +D+DWA W   GSY YR  +Q   D +
Sbjct: 314 AAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFE 373

Query: 379 ESFGIMDSSWTKDRSPRLQQMLQLAKRINQDPN-SKGPMSYIMLHPVSGQCVKLDGKGGI 438
           E++G  +  W++ R+P     L+  ++  QDP  + GP   I+ HP SG CV+      +
Sbjct: 374 ETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVESGIGNTV 433

Query: 439 ELGDCET-PTLWDHTGD--GSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSS-WTVASKA 498
            LG C++  + W++     G    + +  C+ + G+G P +++  CS   ++ W+  S A
Sbjct: 434 HLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSA 493

Query: 499 KLQLSTKSGGEN-----ICLEKESDTSIVVKKCICLKDEWNCFD--DPQSQWFKLVPTN 538
           +LQL T+  G++     +CL+      I   +CIC+ D  +C+   +P+ QWFK++ TN
Sbjct: 494 QLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICITDS-HCYPKLNPEKQWFKVITTN 547

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BLAST of Cucsat.G21085 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 1.1e-10
Identity = 81/371 (21.83%), Postives = 143/371 (38.54%), Query Frame = 0


Query: 41  RWIVDSKTGQRVKLSCVNLVS-HAQSMVAQGLDKRPLKDLANEIVSRDYNCVRLTWSVYM 100
           R IVD  +G+ V+L  VN+      + V  GL  R  KD+  ++    +N VRL +    
Sbjct: 32  RQIVDD-SGKVVQLKGVNVFGFETGNHVMHGLWARNWKDMIVQMQGLGFNAVRLPFC--- 91

Query: 101 FTRYPFETIGDVLD-GLDINKVKNGVKKHNPQFLNMTVTSAFKTVVDGLGNAGLMVILDN 160
               P     D +   +D ++        N     +T       V+      G+ V+LD+
Sbjct: 92  ----PATLRSDTMPASIDYSR--------NADLQGLTSLQILDKVIAEFNARGMYVLLDH 151

Query: 161 HISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAYVARHFSWHPKVVGMSLRNELRGSNNV 220
           H      C  + +    +   ++   +WL  L +VA  +   P V+G+ L+NE  G+   
Sbjct: 152 HTPD---CAGISE---LWYTGSYTEAQWLADLRFVANRYKNVPYVLGLDLKNEPHGAATW 211

Query: 221 GV------WRKYVKLGSHLIHRINPRLLVVISGLN------------YDNDLSYLKKKPL 280
           G       W K  + GS  +  + P+ L+ + G+             +  +L  L   PL
Sbjct: 212 GTGNAATDWNKAAERGSAAVLAVAPKWLIAVEGITDNPVCSTNGGIFWGGNLQPLACTPL 271

Query: 281 GYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIA--CNQVMDKFEREAGFVVDMKDPYPLF 340
                N+++L  H+Y         FV+   N +   N +   +ER  G        + L 
Sbjct: 272 NIPA-NRLLLAPHVYG-----PDVFVQSYFNDSNFPNNMPAIWERHFG---QFAGTHALL 331

Query: 341 LSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWA-YWAFQGSYMYRQGQQDVDESFGIMD 389
           L EFG     G+     +    +  +  K I+   YW++           +  ++ GI+ 
Sbjct: 332 LGEFGGKYGEGDARDKTWQDALVKYLRSKGINQGFYWSW---------NPNSGDTGGILR 362

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BLAST of Cucsat.G21085 vs. NCBI nr
Match: XP_004143963.2 (glycosyl hydrolase 5 family protein [Cucumis sativus])

HSP 1 Score: 1093 bits (2827), Expect = 0.0
Identity = 527/527 (100.00%), Postives = 527/527 (100.00%), Query Frame = 0


Query: 13  MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 72
           MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD
Sbjct: 1   MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 60

Query: 73  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL 132
           KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL
Sbjct: 61  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL 120

Query: 133 NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY 192
           NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY
Sbjct: 121 NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY 180

Query: 193 VARHFSWHPKVVGMSLRNELRGSNNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDL 252
           VARHFSWHPKVVGMSLRNELRGSNNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDL
Sbjct: 181 VARHFSWHPKVVGMSLRNELRGSNNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDL 240

Query: 253 SYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK 312
           SYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK
Sbjct: 241 SYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK 300

Query: 313 DPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDES 372
           DPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDES
Sbjct: 301 DPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDES 360

Query: 373 FGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELG 432
           FGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELG
Sbjct: 361 FGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELG 420

Query: 433 DCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTK 492
           DCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTK
Sbjct: 421 DCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTK 480

Query: 493 SGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 539
           SGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA
Sbjct: 481 SGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 527

[Close]

BLAST of Cucsat.G21085 vs. NCBI nr
Match: XP_008437726.1 (PREDICTED: uncharacterized protein LOC103483073 [Cucumis melo] >KAA0042708.1 major extracellular endoglucanase-like [Cucumis melo var. makuwa] >TYK06112.1 major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1016 bits (2626), Expect = 0.0
Identity = 495/527 (93.93%), Postives = 507/527 (96.20%), Query Frame = 0


Query: 13  MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 72
           MAKAF LIS+LFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD
Sbjct: 1   MAKAFSLISLLFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 60

Query: 73  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL 132
           KRPLKDLANEIVSRDYNCVRLTWSVYMFTR+PFETIGDVLDGLDINKVKNGVKKHNPQ L
Sbjct: 61  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRHPFETIGDVLDGLDINKVKNGVKKHNPQIL 120

Query: 133 NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY 192
           NMTVTSAFKTVVDGLGNAGLMVI+DNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLA+
Sbjct: 121 NMTVTSAFKTVVDGLGNAGLMVIMDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAF 180

Query: 193 VARHFSWHPKVVGMSLRNELRGSNNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDL 252
           V          VGMSLRNELRGSNNV VWRKYVK GSHLIHRINPRLLVVISGLNYDNDL
Sbjct: 181 V----------VGMSLRNELRGSNNVDVWRKYVKQGSHLIHRINPRLLVVISGLNYDNDL 240

Query: 253 SYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK 312
           SYLKKKPLGYNL+NKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK
Sbjct: 241 SYLKKKPLGYNLHNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK 300

Query: 313 DPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDES 372
           +PYPLFLSEFGYDLRG NKAQNRFMSCFLARIIGKDI+WAYWAFQGSYMYRQGQQDVDES
Sbjct: 301 NPYPLFLSEFGYDLRGVNKAQNRFMSCFLARIIGKDIEWAYWAFQGSYMYRQGQQDVDES 360

Query: 373 FGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELG 432
           FGIMDS+WTKDRSPRLQQML+LAKRINQDPNSK P+SYIMLHPVSGQCV+LDGKGGIELG
Sbjct: 361 FGIMDSTWTKDRSPRLQQMLKLAKRINQDPNSKAPLSYIMLHPVSGQCVQLDGKGGIELG 420

Query: 433 DCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTK 492
           DCE PTLWDHTGDGSPMKL NGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQL+TK
Sbjct: 421 DCEKPTLWDHTGDGSPMKLSNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLATK 480

Query: 493 SGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 539
           SGGE ICLEKESDTSIVVKKCICLKDEWNCFDDPQ QWFKLVPTNVA
Sbjct: 481 SGGEKICLEKESDTSIVVKKCICLKDEWNCFDDPQPQWFKLVPTNVA 517

[Close]

BLAST of Cucsat.G21085 vs. NCBI nr
Match: XP_038907081.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 950 bits (2456), Expect = 0.0
Identity = 450/528 (85.23%), Postives = 495/528 (93.75%), Query Frame = 0


Query: 13  MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 72
           MAKA  LIS++  SLLVLSQSLPLST GRWIVDS+TGQRVKLSCVNLVSHAQSM+AQGLD
Sbjct: 1   MAKALSLISLVLFSLLVLSQSLPLSTSGRWIVDSQTGQRVKLSCVNLVSHAQSMLAQGLD 60

Query: 73  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL 132
           KR LKDLAN+I SRD+NCVRLTWS++MFTR+ +ETIGDVLDGLDI KVK+GV+KHNP+ L
Sbjct: 61  KRSLKDLANDISSRDFNCVRLTWSIHMFTRHAYETIGDVLDGLDIGKVKSGVQKHNPEIL 120

Query: 133 NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY 192
           NMTV+ AF+TV++GLGNAGLM++LDNHISQPRWCCSL+DGNGFFGDRNF P EWL+GL Y
Sbjct: 121 NMTVSHAFRTVINGLGNAGLMIVLDNHISQPRWCCSLNDGNGFFGDRNFNPNEWLQGLTY 180

Query: 193 VARHFSWHPKVVGMSLRNELRGSNNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDL 252
           VARHFS  P V+GMSLRNELRGSNNV  W KYV+ GSHLIH INP LLVVISGLNYDNDL
Sbjct: 181 VARHFSCQPHVIGMSLRNELRGSNNVDEWHKYVRRGSHLIHNINPNLLVVISGLNYDNDL 240

Query: 253 SYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK 312
           S+LKKKPLGY L+NKVVLEAHLYSFSG+PESKFVKKPLNIACNQVM+KFEREAGFVVDM+
Sbjct: 241 SHLKKKPLGYKLHNKVVLEAHLYSFSGDPESKFVKKPLNIACNQVMEKFEREAGFVVDME 300

Query: 313 DPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDES 372
           DPYPL++SEFGYD+RGGNKAQNRFM+CFLAR+IGKDIDWA WAFQGSYMYRQGQ+D DES
Sbjct: 301 DPYPLWISEFGYDVRGGNKAQNRFMNCFLARLIGKDIDWALWAFQGSYMYRQGQEDRDES 360

Query: 373 FGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELG 432
           FG+MD++WTKDRSP+L+QMLQLAKRINQDPNSK  MSYIMLHPVSGQCVK DGKGGIELG
Sbjct: 361 FGVMDNTWTKDRSPQLKQMLQLAKRINQDPNSKASMSYIMLHPVSGQCVKSDGKGGIELG 420

Query: 433 DCETPTLWDHTGDGSPMKLW-NGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLST 492
           DC TPTLW+HTGDG+PMKL  NGQCLKSAGDGKPP+VSA+CSGDGS+WTVASKAKLQL+T
Sbjct: 421 DCTTPTLWNHTGDGTPMKLLSNGQCLKSAGDGKPPMVSADCSGDGSNWTVASKAKLQLAT 480

Query: 493 KSGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 539
           KSGGEN CLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA
Sbjct: 481 KSGGENFCLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 528

[Close]

BLAST of Cucsat.G21085 vs. NCBI nr
Match: XP_038907078.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 876 bits (2264), Expect = 0.0
Identity = 415/528 (78.60%), Postives = 470/528 (89.02%), Query Frame = 0


Query: 13  MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 72
           MAKAF LIS++F SLL LSQSL LSTRGRWIVDS+TGQRVKLSCVNLVSH QSM+ QGLD
Sbjct: 1   MAKAFSLISLVFFSLLPLSQSLTLSTRGRWIVDSQTGQRVKLSCVNLVSHGQSMLTQGLD 60

Query: 73  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL 132
           KRPL DLAN+I+SRD+NCVRLTWS++MFTRY   TI DVLD LDI  VK+GV+ HN + L
Sbjct: 61  KRPLNDLANDIISRDFNCVRLTWSIHMFTRYSQMTIVDVLDNLDIKNVKSGVQTHNLEML 120

Query: 133 NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY 192
           NMTV  AFKTV+DGLGNAGLM++LDNHISQPRWCCSL DGNGFFGDR+F  +EWLRGL Y
Sbjct: 121 NMTVAHAFKTVIDGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFHTVEWLRGLTY 180

Query: 193 VARHFSWHPKVVGMSLRNELRGS-NNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDND 252
           VARH+SWHP+V+GMSLRNELRG   N+  W KYV+ GSHLIHR NP LLV+ISG+NYDND
Sbjct: 181 VARHYSWHPQVIGMSLRNELRGPFTNMDKWYKYVRKGSHLIHRENPNLLVMISGINYDND 240

Query: 253 LSYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDM 312
           LS+LKKKPLGYNLNNKVVLEAHLYSFS EPESKF++KPLN+AC+ VM++FEREAGFVVDM
Sbjct: 241 LSFLKKKPLGYNLNNKVVLEAHLYSFSEEPESKFLEKPLNVACDHVMERFEREAGFVVDM 300

Query: 313 KDPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDE 372
           ++PYPL+LSEFGY+  G N+AQNRFMSCFLARIIGKD+DWA WAFQG+YMYRQGQ++ +E
Sbjct: 301 ENPYPLWLSEFGYNQGGANEAQNRFMSCFLARIIGKDLDWALWAFQGTYMYRQGQENAEE 360

Query: 373 SFGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIEL 432
           +FG+MDSSWTKDR+P+L+QMLQLAKRIN DPNS  PM YIMLHPVSGQCV+ D +GGI L
Sbjct: 361 TFGVMDSSWTKDRNPQLKQMLQLAKRINLDPNSNEPMLYIMLHPVSGQCVRSDNEGGIVL 420

Query: 433 GDCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLST 492
           GDC TPT W H+GDGS ++L  G CLKS GDGKPPVVSA+CSGDGS WTVASKAKLQL+T
Sbjct: 421 GDCLTPTRWSHSGDGSLLQLSEGLCLKSVGDGKPPVVSADCSGDGSIWTVASKAKLQLAT 480

Query: 493 KSGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 539
           KSGGEN CLEK+SD+ IVVKKCICLKDE NCFDDPQSQWFKLVPTNVA
Sbjct: 481 KSGGENFCLEKKSDSKIVVKKCICLKDELNCFDDPQSQWFKLVPTNVA 528

[Close]

BLAST of Cucsat.G21085 vs. NCBI nr
Match: KAG6601845.1 (hypothetical protein SDJN03_07078, partial [Cucurbita argyrosperma subsp. sororia] >KAG6601853.1 hypothetical protein SDJN03_07086, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 836 bits (2159), Expect = 8.64e-302
Identity = 399/529 (75.43%), Postives = 459/529 (86.77%), Query Frame = 0


Query: 13  MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 72
           MAK+F LI+++  SL   SQSLPL TRGRWI+D++TG+RVKL+CVNLVSHAQ+MVAQGLD
Sbjct: 1   MAKSFSLIALILFSLFSFSQSLPLCTRGRWIIDAQTGKRVKLNCVNLVSHAQTMVAQGLD 60

Query: 73  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL 132
           KRPLK+LA EI SR +NCVRLTWSV+MFTRY +ETIGDVLDGLDI  VKNGV+KHNP+ L
Sbjct: 61  KRPLKELAAEISSRQFNCVRLTWSVHMFTRYAYETIGDVLDGLDIGDVKNGVEKHNPEIL 120

Query: 133 NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY 192
            M+V  AF+TV++ LG+ G+MVILDNHISQPRWCCSL DGNGFFGDRNF P EW +GL++
Sbjct: 121 KMSVAKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNEWFQGLSF 180

Query: 193 VARHFSWHPKVVGMSLRNELRGS-NNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDND 252
           VA  F+ +P V+GMSLRNELRG   N   W  Y++ GS LIHRINP +L++ISGLNYDND
Sbjct: 181 VATQFTCNPHVIGMSLRNELRGPFTNNDAWCYYMRQGSQLIHRINPNILIIISGLNYDND 240

Query: 253 LSYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDM 312
           LSYLKK+PLGY L+NKVVLEAHLYSFSG+ ESKFVKKPLNI CN++M+KFEREAGFVVDM
Sbjct: 241 LSYLKKRPLGYTLHNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFEREAGFVVDM 300

Query: 313 KDPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDE 372
           ++PYPLFLSEFGYD  GGN+A+NRFMSCFLARI  KDIDWA WAFQGSYMYRQG  D DE
Sbjct: 301 ENPYPLFLSEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQGHTDPDE 360

Query: 373 SFGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIEL 432
           SFG+MD SWTKDR+P+L Q+LQL KRINQDPNSK PMSYIMLH VSGQCV+ D KGGIEL
Sbjct: 361 SFGVMDHSWTKDRNPKLAQLLQLVKRINQDPNSKAPMSYIMLHSVSGQCVRSDNKGGIEL 420

Query: 433 GDCETPTLWDHTGDGSPMKLW-NGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLS 492
           GDC +PT W+HTGD SPM L  NGQCLKSAGDGK P+VSAECSGDGS+WTV+SKAKLQL+
Sbjct: 421 GDCTSPTQWNHTGDASPMMLLSNGQCLKSAGDGKSPMVSAECSGDGSNWTVSSKAKLQLA 480

Query: 493 TKSGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 539
           TK G +N CLEKES+ SIV+KKCICL DE +C DDPQ+QWFKLVPTNV+
Sbjct: 481 TKIGEDNFCLEKESNASIVLKKCICL-DEESCMDDPQTQWFKLVPTNVS 528

[Close]

BLAST of Cucsat.G21085 vs. ExPASy TrEMBL
Match: A0A0A0KNT6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151630 PE=3 SV=1)

HSP 1 Score: 1093 bits (2827), Expect = 0.0
Identity = 527/527 (100.00%), Postives = 527/527 (100.00%), Query Frame = 0


Query: 13  MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 72
           MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD
Sbjct: 1   MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 60

Query: 73  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL 132
           KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL
Sbjct: 61  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL 120

Query: 133 NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY 192
           NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY
Sbjct: 121 NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY 180

Query: 193 VARHFSWHPKVVGMSLRNELRGSNNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDL 252
           VARHFSWHPKVVGMSLRNELRGSNNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDL
Sbjct: 181 VARHFSWHPKVVGMSLRNELRGSNNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDL 240

Query: 253 SYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK 312
           SYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK
Sbjct: 241 SYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK 300

Query: 313 DPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDES 372
           DPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDES
Sbjct: 301 DPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDES 360

Query: 373 FGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELG 432
           FGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELG
Sbjct: 361 FGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELG 420

Query: 433 DCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTK 492
           DCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTK
Sbjct: 421 DCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTK 480

Query: 493 SGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 539
           SGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA
Sbjct: 481 SGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 527

[Close]

BLAST of Cucsat.G21085 vs. ExPASy TrEMBL
Match: A0A5A7TIV3 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G002130 PE=3 SV=1)

HSP 1 Score: 1016 bits (2626), Expect = 0.0
Identity = 495/527 (93.93%), Postives = 507/527 (96.20%), Query Frame = 0


Query: 13  MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 72
           MAKAF LIS+LFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD
Sbjct: 1   MAKAFSLISLLFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 60

Query: 73  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL 132
           KRPLKDLANEIVSRDYNCVRLTWSVYMFTR+PFETIGDVLDGLDINKVKNGVKKHNPQ L
Sbjct: 61  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRHPFETIGDVLDGLDINKVKNGVKKHNPQIL 120

Query: 133 NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY 192
           NMTVTSAFKTVVDGLGNAGLMVI+DNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLA+
Sbjct: 121 NMTVTSAFKTVVDGLGNAGLMVIMDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAF 180

Query: 193 VARHFSWHPKVVGMSLRNELRGSNNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDL 252
           V          VGMSLRNELRGSNNV VWRKYVK GSHLIHRINPRLLVVISGLNYDNDL
Sbjct: 181 V----------VGMSLRNELRGSNNVDVWRKYVKQGSHLIHRINPRLLVVISGLNYDNDL 240

Query: 253 SYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK 312
           SYLKKKPLGYNL+NKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK
Sbjct: 241 SYLKKKPLGYNLHNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK 300

Query: 313 DPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDES 372
           +PYPLFLSEFGYDLRG NKAQNRFMSCFLARIIGKDI+WAYWAFQGSYMYRQGQQDVDES
Sbjct: 301 NPYPLFLSEFGYDLRGVNKAQNRFMSCFLARIIGKDIEWAYWAFQGSYMYRQGQQDVDES 360

Query: 373 FGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELG 432
           FGIMDS+WTKDRSPRLQQML+LAKRINQDPNSK P+SYIMLHPVSGQCV+LDGKGGIELG
Sbjct: 361 FGIMDSTWTKDRSPRLQQMLKLAKRINQDPNSKAPLSYIMLHPVSGQCVQLDGKGGIELG 420

Query: 433 DCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTK 492
           DCE PTLWDHTGDGSPMKL NGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQL+TK
Sbjct: 421 DCEKPTLWDHTGDGSPMKLSNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLATK 480

Query: 493 SGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 539
           SGGE ICLEKESDTSIVVKKCICLKDEWNCFDDPQ QWFKLVPTNVA
Sbjct: 481 SGGEKICLEKESDTSIVVKKCICLKDEWNCFDDPQPQWFKLVPTNVA 517

[Close]

BLAST of Cucsat.G21085 vs. ExPASy TrEMBL
Match: A0A1S3AUC3 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103483073 PE=3 SV=1)

HSP 1 Score: 1016 bits (2626), Expect = 0.0
Identity = 495/527 (93.93%), Postives = 507/527 (96.20%), Query Frame = 0


Query: 13  MAKAFPLISILFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 72
           MAKAF LIS+LFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD
Sbjct: 1   MAKAFSLISLLFLSLLVLSQSLPLSTRGRWIVDSKTGQRVKLSCVNLVSHAQSMVAQGLD 60

Query: 73  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVKKHNPQFL 132
           KRPLKDLANEIVSRDYNCVRLTWSVYMFTR+PFETIGDVLDGLDINKVKNGVKKHNPQ L
Sbjct: 61  KRPLKDLANEIVSRDYNCVRLTWSVYMFTRHPFETIGDVLDGLDINKVKNGVKKHNPQIL 120

Query: 133 NMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAY 192
           NMTVTSAFKTVVDGLGNAGLMVI+DNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLA+
Sbjct: 121 NMTVTSAFKTVVDGLGNAGLMVIMDNHISQPRWCCSLHDGNGFFGDRNFKPIEWLRGLAF 180

Query: 193 VARHFSWHPKVVGMSLRNELRGSNNVGVWRKYVKLGSHLIHRINPRLLVVISGLNYDNDL 252
           V          VGMSLRNELRGSNNV VWRKYVK GSHLIHRINPRLLVVISGLNYDNDL
Sbjct: 181 V----------VGMSLRNELRGSNNVDVWRKYVKQGSHLIHRINPRLLVVISGLNYDNDL 240

Query: 253 SYLKKKPLGYNLNNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK 312
           SYLKKKPLGYNL+NKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK
Sbjct: 241 SYLKKKPLGYNLHNKVVLEAHLYSFSGEPESKFVKKPLNIACNQVMDKFEREAGFVVDMK 300

Query: 313 DPYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSYMYRQGQQDVDES 372
           +PYPLFLSEFGYDLRG NKAQNRFMSCFLARIIGKDI+WAYWAFQGSYMYRQGQQDVDES
Sbjct: 301 NPYPLFLSEFGYDLRGVNKAQNRFMSCFLARIIGKDIEWAYWAFQGSYMYRQGQQDVDES 360

Query: 373 FGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQCVKLDGKGGIELG 432
           FGIMDS+WTKDRSPRLQQML+LAKRINQDPNSK P+SYIMLHPVSGQCV+LDGKGGIELG
Sbjct: 361 FGIMDSTWTKDRSPRLQQMLKLAKRINQDPNSKAPLSYIMLHPVSGQCVQLDGKGGIELG 420

Query: 433 DCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLSTK 492
           DCE PTLWDHTGDGSPMKL NGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQL+TK
Sbjct: 421 DCEKPTLWDHTGDGSPMKLSNGQCLKSAGDGKPPVVSAECSGDGSSWTVASKAKLQLATK 480

Query: 493 SGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 539
           SGGE ICLEKESDTSIVVKKCICLKDEWNCFDDPQ QWFKLVPTNVA
Sbjct: 481 SGGEKICLEKESDTSIVVKKCICLKDEWNCFDDPQPQWFKLVPTNVA 517

[Close]

BLAST of Cucsat.G21085 vs. ExPASy TrEMBL
Match: A0A0A0KQT4 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151620 PE=3 SV=1)

HSP 1 Score: 776 bits (2005), Expect = 1.84e-279
Identity = 368/479 (76.83%), Postives = 419/479 (87.47%), Query Frame = 0


Query: 66  MVAQGLDKRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVK 125
           M+AQGLDKR LKDLAN+I+SRD+NCVRLTWS +MFTRY   TI DVLD LDI  VK+GV+
Sbjct: 1   MLAQGLDKRTLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIIDVLDNLDIQNVKSGVQ 60

Query: 126 KHNPQFLNMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIE 185
           KHNP+ LNMTV  AFKTVV GLGNAGLM++LDNHISQPRWCCSL DGNGFFGDR+F  +E
Sbjct: 61  KHNPKMLNMTVDHAFKTVVYGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDTLE 120

Query: 186 WLRGLAYVARHFSWHPKVVGMSLRNELRGS-NNVGVWRKYVKLGSHLIHRINPRLLVVIS 245
           WL+GL YVARHF+WH +V+GMSLRNELRG   N+  W KYVK GSHLIH  N +LLV+IS
Sbjct: 121 WLQGLDYVARHFTWHSQVIGMSLRNELRGPYTNMDNWYKYVKEGSHLIHTKNRKLLVIIS 180

Query: 246 GLNYDNDLSYLKKKPLGYNLNNKVVLEAHLYSFSGE---PESKFVKKPLNIACNQVMDKF 305
           G+ +DNDLS+LKKK LGYNL+NKVVLEAHLY FSG    PESKFVKKPLNI CNQVM+KF
Sbjct: 181 GITFDNDLSFLKKKSLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNIVCNQVMEKF 240

Query: 306 EREAGFVVDMKD-PYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSY 365
           EREAGFVV+M+D PYPL+LSEFGYDLRG NKAQNRFMSCFLA I+ KD+DWAYWAFQG+Y
Sbjct: 241 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVAKDLDWAYWAFQGTY 300

Query: 366 MYRQGQQDVDESFGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQC 425
           MYRQGQ+ VDE+FG+MDS+WT DR+P+L QMLQLAKRINQDPNS   MSYI+LHPVSGQC
Sbjct: 301 MYRQGQESVDETFGVMDSTWTNDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 360

Query: 426 VKLDGKGGIELGDCETPTLWDHTGDGSPMKLWNGQCLKSAGDGKPPVVSAECSGDGSSWT 485
           ++ +G+GGI LGDC TPT W H+GDGS MKL NGQCL+SAGDGKP +VSAECS DGS WT
Sbjct: 361 IRSNGQGGIVLGDCLTPTHWTHSGDGSSMKLSNGQCLQSAGDGKPLIVSAECSSDGSKWT 420

Query: 486 VASKAKLQLSTKSGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 539
           +ASKAKLQL+TKSGGEN CLEK+S+T IVVK+CICL+DE NCF+DPQ QWFKLVPTNVA
Sbjct: 421 MASKAKLQLATKSGGENFCLEKKSNTKIVVKRCICLEDELNCFNDPQPQWFKLVPTNVA 479

[Close]

BLAST of Cucsat.G21085 vs. ExPASy TrEMBL
Match: A0A5A7TLU0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G002120 PE=3 SV=1)

HSP 1 Score: 773 bits (1996), Expect = 4.48e-278
Identity = 368/480 (76.67%), Postives = 419/480 (87.29%), Query Frame = 0


Query: 66  MVAQGLDKRPLKDLANEIVSRDYNCVRLTWSVYMFTRYPFETIGDVLDGLDINKVKNGVK 125
           M+AQGLDKRPLKDLAN+I+SRD+NCVRLTWS +MFTRY   TI DVLD LDI  VK+GV+
Sbjct: 1   MLAQGLDKRPLKDLANDIISRDFNCVRLTWSTHMFTRYSHMTIVDVLDDLDIQNVKSGVQ 60

Query: 126 KHNPQFLNMTVTSAFKTVVDGLGNAGLMVILDNHISQPRWCCSLHDGNGFFGDRNFKPIE 185
            HNP+ LNMTV  AFKTVVDGLGNAGLM++LDNHISQPRWCCSL DGNGFFGDR+F  +E
Sbjct: 61  THNPEMLNMTVAHAFKTVVDGLGNAGLMIVLDNHISQPRWCCSLDDGNGFFGDRHFDALE 120

Query: 186 WLRGLAYVARHFSWHPKVVGMSLRNELRGS-NNVGVWRKYVKLGSHLIHRINPRLLVVIS 245
           WL+GL YVAR+F+ H +V+GMSLRNELRG   N+  W KYV  GSHLIH  NP LLV+IS
Sbjct: 121 WLQGLDYVARNFTGHSQVIGMSLRNELRGPYTNMDNWYKYVSNGSHLIHTKNPNLLVIIS 180

Query: 246 GLNYDNDLSYLKKKPLGYNLNNKVVLEAHLYSFSGE---PESKFVKKPLNIACNQVMDKF 305
           G+ +D DLS+LK+KPLGYNL+NKVVLEAHLY FSG    PESKFVKKPLN+ CNQV +KF
Sbjct: 181 GVTFDTDLSFLKEKPLGYNLHNKVVLEAHLYPFSGSEKLPESKFVKKPLNVVCNQVKEKF 240

Query: 306 EREAGFVVDMKD-PYPLFLSEFGYDLRGGNKAQNRFMSCFLARIIGKDIDWAYWAFQGSY 365
           EREAGFVV+M+D PYPL+LSEFGYDLRG NKAQNRFMSCFLA I+GKD+DWAYWAFQG+Y
Sbjct: 241 EREAGFVVNMEDEPYPLWLSEFGYDLRGDNKAQNRFMSCFLAHIVGKDLDWAYWAFQGTY 300

Query: 366 MYRQGQQDVDESFGIMDSSWTKDRSPRLQQMLQLAKRINQDPNSKGPMSYIMLHPVSGQC 425
           MYRQGQ+ VDE+FGIMDS+WTKDR+P+L QMLQLAKRINQDPNS   MSYI+LHPVSGQC
Sbjct: 301 MYRQGQESVDEAFGIMDSTWTKDRNPQLNQMLQLAKRINQDPNSNASMSYIILHPVSGQC 360

Query: 426 VKLDGKGGIELGDCETPTLWDHTGDGSPMKLW-NGQCLKSAGDGKPPVVSAECSGDGSSW 485
           ++ +G+GGI LGDC TPT W ++GDGS MKL  NGQCLKSAGDGKPPVVS ECSG+ S W
Sbjct: 361 IRSNGQGGIALGDCLTPTQWTYSGDGSSMKLLSNGQCLKSAGDGKPPVVSTECSGEESKW 420

Query: 486 TVASKAKLQLSTKSGGENICLEKESDTSIVVKKCICLKDEWNCFDDPQSQWFKLVPTNVA 539
           T+ASKAKLQL+TKSGGE+ CLEK+SDT IVVK+CICLKDE NCF+DPQ QWFKLVPTNVA
Sbjct: 421 TMASKAKLQLATKSGGEDFCLEKKSDTKIVVKRCICLKDELNCFNDPQPQWFKLVPTNVA 480

The following BLAST results are available for this feature:

BLAST of Cucsat.G21085 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	1.5e-108	38.22	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P19487	1.1e-10	21.83	Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra...	[more]

BLAST of Cucsat.G21085 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143963.2	0.0	100.00	glycosyl hydrolase 5 family protein [Cucumis sativus]	[more]
XP_008437726.1	0.0	93.93	PREDICTED: uncharacterized protein LOC103483073 [Cucumis melo] >KAA0042708.1 maj...	[more]
XP_038907081.1	0.0	85.23	glycosyl hydrolase 5 family protein-like [Benincasa hispida]	[more]
XP_038907078.1	0.0	78.60	glycosyl hydrolase 5 family protein-like [Benincasa hispida]	[more]
KAG6601845.1	8.64e-302	75.43	hypothetical protein SDJN03_07078, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Cucsat.G21085 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KNT6	0.0	100.00	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151630 PE=3...	[more]
A0A5A7TIV3	0.0	93.93	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3AUC3	0.0	93.93	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103483073 PE=3 SV...	[more]
A0A0A0KQT4	1.84e-279	76.83	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G151620 PE=3...	[more]
A0A5A7TLU0	4.48e-278	76.67	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 33..401 e-value: 6.0E-63 score: 215.2
None	No IPR available	GENE3D	2.80.10.50		coord: 410..521 e-value: 6.6E-5 score: 25.0
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 20..535
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 73..356 e-value: 4.6E-19 score: 68.9
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 407..532
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 34..393

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G21085.T1	Cucsat.G21085.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds