Cucsat.G20714 (gene) Cucumber (B10) v3

BLAST of Cucsat.G20714 vs. ExPASy Swiss-Prot
Match: P98188 (Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.4e-183
Identity = 327/509 (64.24%), Postives = 391/509 (76.82%), Query Frame = 0

Query: 6   LLSFSLFLLPLLFFAFIFTRTPN-----PHFTPPIKLPKSYPIVGSFFALFANRHRRLQW 65
           L+S+ LF   L +F F+ T+T +     P  T    +PKSYPI GS F+L AN HRR+QW
Sbjct: 6   LISWLLFSTSLFWFLFLATKTKSKPPKTPSSTTNTPIPKSYPIFGSAFSLLANFHRRIQW 65

Query: 66  LSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDG 125
            SD+LQ  P++TF LHR FG RQ FTA PAVVQHIL+T F  Y KG +F     DFLGDG
Sbjct: 66  TSDILQTIPSSTFVLHRPFGARQVFTAQPAVVQHILRTNFTCYGKGLTFYQSINDFLGDG 125

Query: 126 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDV 185
           IFNADGESWKFQRQ+SSHEF T+SLRKFVETVVD ELSDRLVP+L  A++S   LD QD+
Sbjct: 126 IFNADGESWKFQRQISSHEFNTRSLRKFVETVVDVELSDRLVPVLSQASNSQTTLDFQDI 185

Query: 186 LQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLN 245
           LQR  FDN+C IAFGYDP YL PS  +  FAKAF+E+ ++S  R+ +LIP +WK+K+FLN
Sbjct: 186 LQRLTFDNICMIAFGYDPEYLLPSLPEIPFAKAFDESSQLSIERLNALIPLLWKVKRFLN 245

Query: 246 IGSEKQLRIAVAEVRGYANKIINDKKAELK---ANSSIDAVDLLSRFLTSGHSNHNFNID 305
           IG E+QL+ AVAEVRG A KI+ +KK ELK     S  ++VDLLSRFL+SGHS+ +F  D
Sbjct: 246 IGVERQLKEAVAEVRGLATKIVKNKKKELKEKALQSESESVDLLSRFLSSGHSDESFVTD 305

Query: 306 IIISFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAA 365
           ++IS ILAG+DTTSAALTW FWLL+KH  VE  I +EI+ KSE + GYDEVKD+VYTHAA
Sbjct: 306 MVISIILAGRDTTSAALTWFFWLLSKHSHVENEILKEITGKSETV-GYDEVKDMVYTHAA 365

Query: 366 LCESMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPE 425
           LCESMRLYPP+PVD K A  DDVLPDGT+V+KG RV YH YAMGR E IWG DWAEFRPE
Sbjct: 366 LCESMRLYPPLPVDTKVAVHDDVLPDGTLVKKGWRVTYHIYAMGRSEKIWGPDWAEFRPE 425

Query: 426 RWLESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVP 485
           RWL    DE G  KW FVG D Y+YPVFQAGPR+C+GKEMAF+QMKR+VAGI+ RFRVVP
Sbjct: 426 RWLSR--DEVG--KWSFVGIDYYSYPVFQAGPRVCIGKEMAFLQMKRVVAGIMGRFRVVP 485

Query: 486 AAEEGVEPRFVQYMTAKMEGGFPVRIKVR 507
           A  EG+EP +  + T+ M+GGFPV+I+ R
Sbjct: 486 AMVEGIEPEYTAHFTSVMKGGFPVKIEKR 509

BLAST of Cucsat.G20714 vs. ExPASy Swiss-Prot
Match: O81117 (Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2)

HSP 1 Score: 619.4 bits (1596), Expect = 3.7e-176
Identity = 308/513 (60.04%), Postives = 388/513 (75.63%), Query Frame = 0

Query: 1   MLAPLLLSFSLFLLPLLFFAFIFTRTPNPHFTPP------IKLPKSYPIVGSFFALFANR 60
           +L P LL   L +LP   F   F   PN   +        I LPKSYP++GS+ +   N 
Sbjct: 8   VLLPYLLPLLLLILPTTIF---FLTKPNNKVSSTSTNNNIITLPKSYPLIGSYLSFRKNL 67

Query: 61  HRRLQWLSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFT 120
           HRR+QWLSD++QISP+ATF L    G+RQ  T NP+ VQHILK +F  YQKG +F +  +
Sbjct: 68  HRRIQWLSDIVQISPSATFQLDGTLGKRQIITGNPSTVQHILKNQFSNYQKGTTFTNTLS 127

Query: 121 DFLGDGIFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCV 180
           DFLG GIFN +G +WKFQRQV+SHEF TKS+R FVE +VD EL++RL+PIL ++  +  +
Sbjct: 128 DFLGTGIFNTNGPNWKFQRQVASHEFNTKSIRNFVEHIVDTELTNRLIPILTSSTQTNNI 187

Query: 181 LDLQDVLQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWK 240
           LD QD+LQRF FDN+C IAFGYDP YL+PS  +SKFA+A+E+A  ISS R +  +P +WK
Sbjct: 188 LDFQDILQRFTFDNICNIAFGYDPEYLTPSTNRSKFAEAYEDATEISSKRFRLPLPIIWK 247

Query: 241 LKKFLNIGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNF 300
           +KK+ NIGSEK+L+ AV EVR +A K++ +KK EL+  SS++  D+LSRFL+SGHS+ +F
Sbjct: 248 IKKYFNIGSEKRLKEAVTEVRSFAKKLVREKKRELEEKSSLETEDMLSRFLSSGHSDEDF 307

Query: 301 NIDIIISFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYT 360
             DI+ISFILAG+DTTSAALTW FWLL K+P+VE  I  E+S+KSE L  YDEVK++VYT
Sbjct: 308 VADIVISFILAGKDTTSAALTWFFWLLWKNPRVEEEIVNELSKKSE-LMVYDEVKEMVYT 367

Query: 361 HAALCESMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEF 420
           HAAL ESMRLYPPVP+D K+A  DDVLPDG VV+KG  V YH YAMGRM+ +WG DWAEF
Sbjct: 368 HAALSESMRLYPPVPMDSKEAVNDDVLPDGWVVKKGTIVTYHVYAMGRMKSLWGDDWAEF 427

Query: 421 RPERWLESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFR 480
           RPERWLE   DE    KW FVGRD+Y+YPVFQAGPR+CLGKEMAFMQMKRIVAGIV +F+
Sbjct: 428 RPERWLEK--DEVNG-KWVFVGRDSYSYPVFQAGPRVCLGKEMAFMQMKRIVAGIVGKFK 487

Query: 481 VVPAAEEGVEPRFVQYMTAKMEGGFPVRIKVRE 508
           VVP A    EP F+ +++++MEGGFPV I+ R+
Sbjct: 488 VVPEAHLAQEPGFISFLSSQMEGGFPVTIQKRD 513

BLAST of Cucsat.G20714 vs. ExPASy Swiss-Prot
Match: Q9FMV7 (Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 3.7e-128
Identity = 236/507 (46.55%), Postives = 328/507 (64.69%), Query Frame = 0

Query: 5   LLLSFSLFLLPLLFFAFIF---TRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQWL 64
           +L +  L L P++ F  IF   T+T       P   P SY ++GS  +   NRHR LQW 
Sbjct: 3   MLNAIILILFPIIGFVLIFSFPTKTLKAKTASPSN-PTSYQLIGSILSFNKNRHRLLQWY 62

Query: 65  SDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDGI 124
           +D+L++SP+ T T+  LFG+R   TANP  V+HILKT F+ + KG  F  +  D LG GI
Sbjct: 63  TDLLRLSPSQTITVDLLFGRRTIITANPENVEHILKTNFYNFPKGKPFTDLLGDLLGGGI 122

Query: 125 FNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDVL 184
           FN+DGE W  QR+++SHEFT +SLR+F   ++  E+ +RL+P+L +A      +D Q+VL
Sbjct: 123 FNSDGELWSSQRKLASHEFTMRSLREFTFEILREEVQNRLIPVLSSAVDCGETVDFQEVL 182

Query: 185 QRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLNI 244
           +RFAFD VCK++ G+DP  L  +    +  KAF+ A  IS+ R    +  VWK+K+FLN+
Sbjct: 183 KRFAFDVVCKVSLGWDPDCLDLTRPVPELVKAFDVAAEISARRATEPVYAVWKVKRFLNV 242

Query: 245 GSEKQLRIAVAEVRGYANKIINDKKAELKANSSI-DAVDLLSRFLTSGHSNHNFNIDIII 304
           GSEK+LR A+  V    ++II  KK  L     + D  DLLSRFL +GH       D +I
Sbjct: 243 GSEKRLREAIKTVHLSVSEIIRAKKKSLDIGGDVSDKQDLLSRFLAAGHGEEAVR-DSVI 302

Query: 305 SFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALCE 364
           SFI+AG+DTTSAA+TWLFWLL+++  VET+I +E+  K     G+++++++ YT A LCE
Sbjct: 303 SFIMAGRDTTSAAMTWLFWLLSQNDDVETKILDELRNKGSLGLGFEDLREMSYTKACLCE 362

Query: 365 SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWL 424
           +MRLYPPV  D K AA DD+LPDGT ++KG++V Y PY MGRME +WGKDW EF+P RW 
Sbjct: 363 AMRLYPPVAWDSKHAANDDILPDGTPLKKGDKVTYFPYGMGRMEKVWGKDWDEFKPNRWF 422

Query: 425 ESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAAE 484
           E   + +   K       ++ +PVFQAGPR+C+GKEMAF QMK +V  ++ RF+++P   
Sbjct: 423 EE--EPSYGTKPVLKSVSSFKFPVFQAGPRVCIGKEMAFTQMKYVVGSVLSRFKIIPVCN 482

Query: 485 EGVEPRFVQYMTAKMEGGFPVRIKVRE 508
               P FV  +TA M GG  V+IK RE
Sbjct: 483 N--RPVFVPLLTAHMAGGLKVKIKRRE 503

BLAST of Cucsat.G20714 vs. ExPASy Swiss-Prot
Match: Q9SMP5 (Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 3.0e-122
Identity = 236/507 (46.55%), Postives = 324/507 (63.91%), Query Frame = 0

Query: 6   LLSFSL--FLLPLLFFAFIFTR---TPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQW 65
           LLSF +  FL+ ++FF    +      N  + PP     SYP++GS  +   NRHR LQW
Sbjct: 4   LLSFLILAFLITIIFFLSSSSTKKVQENTTYGPP-----SYPLIGSILSFNKNRHRLLQW 63

Query: 66  LSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDG 125
            +++L++SP+ T  +  L  +R   T NP  V++ILKT F  + KG  F  +  D LG G
Sbjct: 64  YTELLRLSPSQTILVPLLGNRRTIITTNPLNVEYILKTNFFNFPKGKPFTDLLGDLLGGG 123

Query: 126 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDV 185
           IFN DG SW  QR+++SHEF+T+SLR F   V+  E+ +RLVP+L TAA     +DLQDV
Sbjct: 124 IFNVDGHSWSSQRKLASHEFSTRSLRSFAFEVLKDEVENRLVPVLSTAADVGTTVDLQDV 183

Query: 186 LQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLN 245
           L+RFAFD VCK++ G+DP  L  +   +   +AF+ A  IS+ R    I  VWK K+ LN
Sbjct: 184 LKRFAFDVVCKVSLGWDPDCLDLTRPVNPLVEAFDTAAEISARRATEPIYAVWKTKRVLN 243

Query: 246 IGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDA-VDLLSRFLTSGHSNHNFNIDII 305
           +GSE++LR A+  V    ++I+  KK  L+  +  +A  DLLSRFL +GH+      D++
Sbjct: 244 VGSERKLREAIRTVHVLVSEIVRAKKKSLEIGTGAEAKQDLLSRFLAAGHNGEAVR-DMV 303

Query: 306 ISFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALC 365
           ISFI+AG+DTTSAA+TWLFWLL ++  VE +I EE+        G++++K++ YT A LC
Sbjct: 304 ISFIMAGRDTTSAAMTWLFWLLTENDDVERKILEEVDPLVSLGLGFEDLKEMAYTKACLC 363

Query: 366 ESMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERW 425
           E+MRLYPPV  D K AA DDVLPDGT V++G++V Y PY MGRME +WG D  EF P RW
Sbjct: 364 EAMRLYPPVSWDSKHAANDDVLPDGTRVKRGDKVTYFPYGMGRMETLWGTDSEEFNPNRW 423

Query: 426 LESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAA 485
            +S   E G+ +        Y +PVFQAGPR+C+GKEMAFMQMK +V  ++ RF +VP  
Sbjct: 424 FDS---EPGSTRPVLKPISPYKFPVFQAGPRVCVGKEMAFMQMKYVVGSVLSRFEIVPVN 483

Query: 486 EEGVEPRFVQYMTAKMEGGFPVRIKVR 507
           ++   P FV  +TA M GG  V+IK R
Sbjct: 484 KD--RPVFVPLLTAHMAGGLKVKIKRR 499

BLAST of Cucsat.G20714 vs. ExPASy Swiss-Prot
Match: Q9ZUX1 (Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.6e-99
Identity = 201/455 (44.18%), Postives = 281/455 (61.76%), Query Frame = 0

Query: 60  WLSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGD 119
           W + +L+ SP +T  +H L       TANP+ V+HILKT FH Y KG  F  I  D LG 
Sbjct: 55  WYTHLLRRSPTSTIKVHVL---NSVITANPSNVEHILKTNFHNYPKGKQFSVILGDLLGR 114

Query: 120 GIFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASS-CCVLDLQ 179
           GIFN+DG++W+FQR+++S E  + S+R F   +V  E+  RL+PIL + + +   VLDLQ
Sbjct: 115 GIFNSDGDTWRFQRKLASLELGSVSVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDLQ 174

Query: 180 DVLQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKF 239
           DV +RF+FD + K++FG+DP  L   F  S+FA AF+ A  +S+ R  +  P +WK K+ 
Sbjct: 175 DVFRRFSFDTISKLSFGFDPDCLRLPFPISEFAVAFDTASLLSAKRALAPFPLLWKTKRL 234

Query: 240 LNIGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFL-TSGHSNHNFNID 299
           L IGSEK+L+ ++  +    N++  D   + +    +   DL+SRF+      +  +  D
Sbjct: 235 LRIGSEKKLQESINVI----NRLAGDLIKQRRLTGLMGKNDLISRFMAVVAEDDDEYLRD 294

Query: 300 IIISFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFG-----YDEVKDLV 359
           I++SF+LAG+DT +A LT  FWLL +HP+VE RI EE+ +     F       DE++++ 
Sbjct: 295 IVVSFLLAGRDTVAAGLTGFFWLLTRHPEVENRIREELDRVMGTGFDSVTARCDEMREMD 354

Query: 360 YTHAALCESMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWA 419
           Y HA+L ESMRL+PPV  D K A  DDVL DGT V  G RV YH YAMGRM+ IWG D+ 
Sbjct: 355 YLHASLYESMRLFPPVQFDSKFALNDDVLSDGTFVNSGTRVTYHAYAMGRMDRIWGPDYE 414

Query: 420 EFRPERWLESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKR 479
           EF+PERWL++ G        +F   +   YPVFQAG R+C+GKEMA M+MK I   I++R
Sbjct: 415 EFKPERWLDNEG--------KFRPENPVKYPVFQAGARVCIGKEMAIMEMKSIAVAIIRR 474

Query: 480 FRVVPAAEEGVEP-RFVQYMTAKMEGGFPVRIKVR 507
           F    A+ E  E  RF   +TA + GG PV I+ R
Sbjct: 475 FETRVASPETTETLRFAPGLTATVNGGLPVMIQER 494

BLAST of Cucsat.G20714 vs. NCBI nr
Match: XP_011658957.1 (cytochrome P450 94A2 [Cucumis sativus] >KGN44126.1 hypothetical protein Csa_016676 [Cucumis sativus])

HSP 1 Score: 1023 bits (2646), Expect = 0.0
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0

Query: 1   MLAPLLLSFSLFLLPLLFFAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQW 60
           MLAPLLLSFSLFLLPLLFFAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQW
Sbjct: 1   MLAPLLLSFSLFLLPLLFFAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQW 60

Query: 61  LSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDG 120
           LSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDG
Sbjct: 61  LSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDG 120

Query: 121 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDV 180
           IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDV
Sbjct: 121 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDV 180

Query: 181 LQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLN 240
           LQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLN
Sbjct: 181 LQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLN 240

Query: 241 IGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDIII 300
           IGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDIII
Sbjct: 241 IGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDIII 300

Query: 301 SFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALCE 360
           SFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALCE
Sbjct: 301 SFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALCE 360

Query: 361 SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWL 420
           SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWL
Sbjct: 361 SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWL 420

Query: 421 ESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAAE 480
           ESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAAE
Sbjct: 421 ESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAAE 480

Query: 481 EGVEPRFVQYMTAKMEGGFPVRIKVREGSE 510
           EGVEPRFVQYMTAKMEGGFPVRIKVREGSE
Sbjct: 481 EGVEPRFVQYMTAKMEGGFPVRIKVREGSE 510

BLAST of Cucsat.G20714 vs. NCBI nr
Match: XP_008447772.1 (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 94A2-like [Cucumis melo])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 476/510 (93.33%), Postives = 492/510 (96.47%), Query Frame = 0

Query: 1   MLAPLLLSFSLFLLPLLFFAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQW 60
           MLA LLLSFSLFLLPL+FFAFIFT+TPNPHFTPPIKLPKSYPIVGSFFA+FANR RRLQW
Sbjct: 1   MLAQLLLSFSLFLLPLIFFAFIFTKTPNPHFTPPIKLPKSYPIVGSFFAVFANRSRRLQW 60

Query: 61  LSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDG 120
           LSDVLQISPAATFTLHRLFGQRQ FTANPAVVQHILKTKFHIYQKGDSFRS+FTDFLGDG
Sbjct: 61  LSDVLQISPAATFTLHRLFGQRQVFTANPAVVQHILKTKFHIYQKGDSFRSVFTDFLGDG 120

Query: 121 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDV 180
           IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELS+RLVPIL+TAAS+C VLDLQDV
Sbjct: 121 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSERLVPILYTAASTCSVLDLQDV 180

Query: 181 LQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLN 240
           LQRFAFDNVCKIAFGYDPAYLSPSF QSKFA+AFEEAVRISSLR QSL+P VWKLKKFLN
Sbjct: 181 LQRFAFDNVCKIAFGYDPAYLSPSFGQSKFARAFEEAVRISSLRFQSLLPIVWKLKKFLN 240

Query: 241 IGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDIII 300
           +GSEKQLRIA+AEVRGYANKII DK+ ELKANSSIDAVDLLSRFLTSGHSNHNF +DIII
Sbjct: 241 VGSEKQLRIAIAEVRGYANKIIKDKETELKANSSIDAVDLLSRFLTSGHSNHNFIVDIII 300

Query: 301 SFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALCE 360
           SFILAGQDTTSAALTW FWLLAK+PQVETRIF+EISQK E LFGYDEVKDLVYTHAALCE
Sbjct: 301 SFILAGQDTTSAALTWFFWLLAKYPQVETRIFQEISQKPEDLFGYDEVKDLVYTHAALCE 360

Query: 361 SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWL 420
           SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEG WGKDWAEFRPERWL
Sbjct: 361 SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGFWGKDWAEFRPERWL 420

Query: 421 ESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAAE 480
           ES GDE G VKWRFVGRDNYTYPVFQAGPRICLGKEMAF+QMKRIVAGIVKRFRVV AAE
Sbjct: 421 ESCGDEEGPVKWRFVGRDNYTYPVFQAGPRICLGKEMAFLQMKRIVAGIVKRFRVVLAAE 480

Query: 481 EGVEPRFVQYMTAKMEGGFPVRIKVREGSE 510
           EGVEPRFVQYMTAKMEGGFPVRIKVREGSE
Sbjct: 481 EGVEPRFVQYMTAKMEGGFPVRIKVREGSE 510

BLAST of Cucsat.G20714 vs. NCBI nr
Match: XP_038887465.1 (cytochrome P450 94A2-like [Benincasa hispida])

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 449/511 (87.87%), Postives = 470/511 (91.98%), Query Frame = 0

Query: 1   MLAPLLLSFSLFLLPLLFFAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQW 60
           MLAPLLL   LFLLPLLFF FIFT+TPN HFTP IKLP+SYP+VGSFFA+FANRHRRLQW
Sbjct: 1   MLAPLLLFIFLFLLPLLFFIFIFTKTPNSHFTPSIKLPRSYPLVGSFFAVFANRHRRLQW 60

Query: 61  LSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDG 120
           LSDVLQ+SPAATFTLHRLFG+RQ FTANPAVVQHILKT+FHIYQKGDS RS F+DFLGDG
Sbjct: 61  LSDVLQVSPAATFTLHRLFGRRQIFTANPAVVQHILKTQFHIYQKGDSIRSPFSDFLGDG 120

Query: 121 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDV 180
           IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPIL+TAA+ C VLD QDV
Sbjct: 121 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILYTAATDCSVLDFQDV 180

Query: 181 LQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLN 240
           LQRFAFDNVCKIAFGYDPAYLSPSF QSKFAKAFEEAVRISSLR QSL+P VWKLKKFL+
Sbjct: 181 LQRFAFDNVCKIAFGYDPAYLSPSFGQSKFAKAFEEAVRISSLRFQSLLPIVWKLKKFLD 240

Query: 241 IGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDIII 300
           IGSEKQLRIA+AEVR YAN II DKKAELK NSSI AVDLLSRFLTSGHS+ NF  DIII
Sbjct: 241 IGSEKQLRIAIAEVRSYANNIIKDKKAELKTNSSIAAVDLLSRFLTSGHSDQNFITDIII 300

Query: 301 SFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALCE 360
           SFILAGQDTTSAALTW FWLLAK+PQVE RI EEISQK+E LF YDEVKDL YTHAALCE
Sbjct: 301 SFILAGQDTTSAALTWFFWLLAKYPQVEIRILEEISQKTEDLFSYDEVKDLTYTHAALCE 360

Query: 361 SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWL 420
           SMRLYPPVP+DGKQ AADDVLPDGTVVRKGERVAYHPYAMGRME IWGKDWAEFRPERWL
Sbjct: 361 SMRLYPPVPMDGKQTAADDVLPDGTVVRKGERVAYHPYAMGRMETIWGKDWAEFRPERWL 420

Query: 421 ESGG-DEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAA 480
           E G  DE   VKWRFVGRDNYTYPVFQAGPRICLGKEMAF+QMKR+VAGI+KRFRVVPA 
Sbjct: 421 ERGSADEVATVKWRFVGRDNYTYPVFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAV 480

Query: 481 EEGVEPRFVQYMTAKMEGGFPVRIKVREGSE 510
             GVEPRFVQYMTAKMEGGFPVRI+VREG E
Sbjct: 481 AVGVEPRFVQYMTAKMEGGFPVRIEVREGLE 511

BLAST of Cucsat.G20714 vs. NCBI nr
Match: XP_022931203.1 (cytochrome P450 94A2-like [Cucurbita moschata])

HSP 1 Score: 844 bits (2181), Expect = 6.30e-306
Identity = 425/507 (83.83%), Postives = 458/507 (90.34%), Query Frame = 0

Query: 3   APLLLSFS-LFLLPLLF--FAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQ 62
           APLLLSF  LFLLPLLF  F FIFT+TP    TP IKLP+SYP+VGSFFA+F NRHRRL+
Sbjct: 3   APLLLSFLFLFLLPLLFSFFFFIFTKTPTSSSTPSIKLPRSYPLVGSFFAVFVNRHRRLE 62

Query: 63  WLSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGD 122
           WLSD+LQ+SP ATFTLHRLFGQ Q FTANPAVVQHILKT+FHIYQKGD FRS  +DFLGD
Sbjct: 63  WLSDILQVSPNATFTLHRLFGQAQVFTANPAVVQHILKTQFHIYQKGDPFRSALSDFLGD 122

Query: 123 GIFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQD 182
           GIFNADGESWKFQRQVSSHEF+TKSLRKFVETVVDAELSDRLVP+L+TAA++C VLD QD
Sbjct: 123 GIFNADGESWKFQRQVSSHEFSTKSLRKFVETVVDAELSDRLVPVLNTAATACSVLDFQD 182

Query: 183 VLQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFL 242
           VLQRFAFDNVCKIAFGYDP+YLSPSF QSKFAKAFE+AVRISSLR QSL+P  WKLKKFL
Sbjct: 183 VLQRFAFDNVCKIAFGYDPSYLSPSFGQSKFAKAFEDAVRISSLRFQSLVPIFWKLKKFL 242

Query: 243 NIGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDII 302
           +IGSEK+LRIAVAEVRGYA  II +KK EL+ NSSI +VDLLSRFL+SGHS+ +F  DII
Sbjct: 243 DIGSEKRLRIAVAEVRGYAKNIIEEKKNELQTNSSIASVDLLSRFLSSGHSDEDFVTDII 302

Query: 303 ISFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALC 362
           ISFILAGQDTTSAALTW FWLL K+PQVE RI +EI  KSE  FGYDEVK+L YTHAALC
Sbjct: 303 ISFILAGQDTTSAALTWFFWLLPKYPQVEDRILDEICNKSED-FGYDEVKNLTYTHAALC 362

Query: 363 ESMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERW 422
           ESMRLYPPVPVDGKQA ADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERW
Sbjct: 363 ESMRLYPPVPVDGKQAVADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERW 422

Query: 423 LESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAA 482
           LESG D A   KWRFVGRDNYTYPVFQAGPRICLGKEMAF+QMKR+VAGI+KRFRVVPAA
Sbjct: 423 LESGDDAA--AKWRFVGRDNYTYPVFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAA 482

Query: 483 EEGVEPRFVQYMTAKMEGGFPVRIKVR 506
            EGVEP+FVQYMTAKMEGGFPVRI+ R
Sbjct: 483 AEGVEPKFVQYMTAKMEGGFPVRIEER 506

BLAST of Cucsat.G20714 vs. NCBI nr
Match: KAG6606392.1 (Cytochrome P450 94A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 844 bits (2180), Expect = 9.65e-306
Identity = 425/509 (83.50%), Postives = 458/509 (89.98%), Query Frame = 0

Query: 3   APLLLSFS-LFLLPLLF----FAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRR 62
           APLLLSF  LFLLPLLF    F FIFT+TP    TP IKLP+SYP+VGSFFA+F NRHRR
Sbjct: 3   APLLLSFLFLFLLPLLFYFFFFFFIFTKTPTSSSTPSIKLPRSYPLVGSFFAVFVNRHRR 62

Query: 63  LQWLSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFL 122
           L+WLSD+LQ+SP ATFTLHRLFGQ Q FTANPAVVQHILKT+FHIYQKGD FRS  +DFL
Sbjct: 63  LEWLSDILQVSPNATFTLHRLFGQAQVFTANPAVVQHILKTQFHIYQKGDPFRSALSDFL 122

Query: 123 GDGIFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDL 182
           GDGIFNADGESWKFQRQVSSHEF+TKSLRKFVETVVDAELSDRLVP+L+TAA++C VLD 
Sbjct: 123 GDGIFNADGESWKFQRQVSSHEFSTKSLRKFVETVVDAELSDRLVPVLNTAATACSVLDF 182

Query: 183 QDVLQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKK 242
           QDVLQRFAFDNVCKIAFGYDPAYLSPSF QS+FAKAFE+AVRISSLR QSL+P  WKLKK
Sbjct: 183 QDVLQRFAFDNVCKIAFGYDPAYLSPSFGQSQFAKAFEDAVRISSLRFQSLVPIFWKLKK 242

Query: 243 FLNIGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNID 302
           FL+IGSEK+LRIAVAEVRGYA  II +KK EL+ NSSI +VDLLSRFL+SGHS+ +F  D
Sbjct: 243 FLDIGSEKRLRIAVAEVRGYAKNIIEEKKNELQTNSSIASVDLLSRFLSSGHSDEDFVTD 302

Query: 303 IIISFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAA 362
           IIISFILAGQDTTSAALTW FWLL K+PQVE RI +EI  KSE  FGYDEVK+L YTHAA
Sbjct: 303 IIISFILAGQDTTSAALTWFFWLLPKYPQVEDRILDEICNKSED-FGYDEVKNLTYTHAA 362

Query: 363 LCESMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPE 422
           LCESMRLYPPVPVDGKQA ADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPE
Sbjct: 363 LCESMRLYPPVPVDGKQAVADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPE 422

Query: 423 RWLESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVP 482
           RWLESG D A   KWRFVGRDNYTYPVFQAGPRICLGKEMAF+QMKR+VAGI+KRFRVVP
Sbjct: 423 RWLESGDDAA--AKWRFVGRDNYTYPVFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVP 482

Query: 483 AAEEGVEPRFVQYMTAKMEGGFPVRIKVR 506
           AA EGVEP+FVQYMTAKMEGGFPVRI+ R
Sbjct: 483 AAAEGVEPKFVQYMTAKMEGGFPVRIEAR 508

BLAST of Cucsat.G20714 vs. ExPASy TrEMBL
Match: A0A0A0K8L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G198310 PE=3 SV=1)

HSP 1 Score: 1023 bits (2646), Expect = 0.0
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0

Query: 1   MLAPLLLSFSLFLLPLLFFAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQW 60
           MLAPLLLSFSLFLLPLLFFAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQW
Sbjct: 1   MLAPLLLSFSLFLLPLLFFAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQW 60

Query: 61  LSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDG 120
           LSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDG
Sbjct: 61  LSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDG 120

Query: 121 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDV 180
           IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDV
Sbjct: 121 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDV 180

Query: 181 LQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLN 240
           LQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLN
Sbjct: 181 LQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLN 240

Query: 241 IGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDIII 300
           IGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDIII
Sbjct: 241 IGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDIII 300

Query: 301 SFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALCE 360
           SFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALCE
Sbjct: 301 SFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALCE 360

Query: 361 SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWL 420
           SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWL
Sbjct: 361 SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWL 420

Query: 421 ESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAAE 480
           ESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAAE
Sbjct: 421 ESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAAE 480

Query: 481 EGVEPRFVQYMTAKMEGGFPVRIKVREGSE 510
           EGVEPRFVQYMTAKMEGGFPVRIKVREGSE
Sbjct: 481 EGVEPRFVQYMTAKMEGGFPVRIKVREGSE 510

BLAST of Cucsat.G20714 vs. ExPASy TrEMBL
Match: A0A1S3BHL2 (LOW QUALITY PROTEIN: cytochrome P450 94A2-like OS=Cucumis melo OX=3656 GN=LOC103490164 PE=3 SV=1)

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 476/510 (93.33%), Postives = 492/510 (96.47%), Query Frame = 0

Query: 1   MLAPLLLSFSLFLLPLLFFAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQW 60
           MLA LLLSFSLFLLPL+FFAFIFT+TPNPHFTPPIKLPKSYPIVGSFFA+FANR RRLQW
Sbjct: 1   MLAQLLLSFSLFLLPLIFFAFIFTKTPNPHFTPPIKLPKSYPIVGSFFAVFANRSRRLQW 60

Query: 61  LSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDG 120
           LSDVLQISPAATFTLHRLFGQRQ FTANPAVVQHILKTKFHIYQKGDSFRS+FTDFLGDG
Sbjct: 61  LSDVLQISPAATFTLHRLFGQRQVFTANPAVVQHILKTKFHIYQKGDSFRSVFTDFLGDG 120

Query: 121 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDV 180
           IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELS+RLVPIL+TAAS+C VLDLQDV
Sbjct: 121 IFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSERLVPILYTAASTCSVLDLQDV 180

Query: 181 LQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLN 240
           LQRFAFDNVCKIAFGYDPAYLSPSF QSKFA+AFEEAVRISSLR QSL+P VWKLKKFLN
Sbjct: 181 LQRFAFDNVCKIAFGYDPAYLSPSFGQSKFARAFEEAVRISSLRFQSLLPIVWKLKKFLN 240

Query: 241 IGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDIII 300
           +GSEKQLRIA+AEVRGYANKII DK+ ELKANSSIDAVDLLSRFLTSGHSNHNF +DIII
Sbjct: 241 VGSEKQLRIAIAEVRGYANKIIKDKETELKANSSIDAVDLLSRFLTSGHSNHNFIVDIII 300

Query: 301 SFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALCE 360
           SFILAGQDTTSAALTW FWLLAK+PQVETRIF+EISQK E LFGYDEVKDLVYTHAALCE
Sbjct: 301 SFILAGQDTTSAALTWFFWLLAKYPQVETRIFQEISQKPEDLFGYDEVKDLVYTHAALCE 360

Query: 361 SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWL 420
           SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEG WGKDWAEFRPERWL
Sbjct: 361 SMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGFWGKDWAEFRPERWL 420

Query: 421 ESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAAE 480
           ES GDE G VKWRFVGRDNYTYPVFQAGPRICLGKEMAF+QMKRIVAGIVKRFRVV AAE
Sbjct: 421 ESCGDEEGPVKWRFVGRDNYTYPVFQAGPRICLGKEMAFLQMKRIVAGIVKRFRVVLAAE 480

Query: 481 EGVEPRFVQYMTAKMEGGFPVRIKVREGSE 510
           EGVEPRFVQYMTAKMEGGFPVRIKVREGSE
Sbjct: 481 EGVEPRFVQYMTAKMEGGFPVRIKVREGSE 510

BLAST of Cucsat.G20714 vs. ExPASy TrEMBL
Match: A0A6J1EXS7 (cytochrome P450 94A2-like OS=Cucurbita moschata OX=3662 GN=LOC111437452 PE=3 SV=1)

HSP 1 Score: 844 bits (2181), Expect = 3.05e-306
Identity = 425/507 (83.83%), Postives = 458/507 (90.34%), Query Frame = 0

Query: 3   APLLLSFS-LFLLPLLF--FAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQ 62
           APLLLSF  LFLLPLLF  F FIFT+TP    TP IKLP+SYP+VGSFFA+F NRHRRL+
Sbjct: 3   APLLLSFLFLFLLPLLFSFFFFIFTKTPTSSSTPSIKLPRSYPLVGSFFAVFVNRHRRLE 62

Query: 63  WLSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGD 122
           WLSD+LQ+SP ATFTLHRLFGQ Q FTANPAVVQHILKT+FHIYQKGD FRS  +DFLGD
Sbjct: 63  WLSDILQVSPNATFTLHRLFGQAQVFTANPAVVQHILKTQFHIYQKGDPFRSALSDFLGD 122

Query: 123 GIFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQD 182
           GIFNADGESWKFQRQVSSHEF+TKSLRKFVETVVDAELSDRLVP+L+TAA++C VLD QD
Sbjct: 123 GIFNADGESWKFQRQVSSHEFSTKSLRKFVETVVDAELSDRLVPVLNTAATACSVLDFQD 182

Query: 183 VLQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFL 242
           VLQRFAFDNVCKIAFGYDP+YLSPSF QSKFAKAFE+AVRISSLR QSL+P  WKLKKFL
Sbjct: 183 VLQRFAFDNVCKIAFGYDPSYLSPSFGQSKFAKAFEDAVRISSLRFQSLVPIFWKLKKFL 242

Query: 243 NIGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDII 302
           +IGSEK+LRIAVAEVRGYA  II +KK EL+ NSSI +VDLLSRFL+SGHS+ +F  DII
Sbjct: 243 DIGSEKRLRIAVAEVRGYAKNIIEEKKNELQTNSSIASVDLLSRFLSSGHSDEDFVTDII 302

Query: 303 ISFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYTHAALC 362
           ISFILAGQDTTSAALTW FWLL K+PQVE RI +EI  KSE  FGYDEVK+L YTHAALC
Sbjct: 303 ISFILAGQDTTSAALTWFFWLLPKYPQVEDRILDEICNKSED-FGYDEVKNLTYTHAALC 362

Query: 363 ESMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERW 422
           ESMRLYPPVPVDGKQA ADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERW
Sbjct: 363 ESMRLYPPVPVDGKQAVADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERW 422

Query: 423 LESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAA 482
           LESG D A   KWRFVGRDNYTYPVFQAGPRICLGKEMAF+QMKR+VAGI+KRFRVVPAA
Sbjct: 423 LESGDDAA--AKWRFVGRDNYTYPVFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAA 482

Query: 483 EEGVEPRFVQYMTAKMEGGFPVRIKVR 506
            EGVEP+FVQYMTAKMEGGFPVRI+ R
Sbjct: 483 AEGVEPKFVQYMTAKMEGGFPVRIEER 506

BLAST of Cucsat.G20714 vs. ExPASy TrEMBL
Match: A0A6J1K0F2 (cytochrome P450 94A2-like OS=Cucurbita maxima OX=3661 GN=LOC111491315 PE=3 SV=1)

HSP 1 Score: 842 bits (2174), Expect = 3.55e-305
Identity = 424/512 (82.81%), Postives = 458/512 (89.45%), Query Frame = 0

Query: 1   MLAPLLLSFSLFLLPLLFFAF------IFTRTPNPHFTPPIKLPKSYPIVGSFFALFANR 60
           M+APLLLSF   LLPLLFF F      IFT+TP    TP IKLP++YP+VGSFFA+F NR
Sbjct: 1   MVAPLLLSF---LLPLLFFFFFFFFFFIFTKTPTSSSTPSIKLPRAYPLVGSFFAVFVNR 60

Query: 61  HRRLQWLSDVLQISPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFT 120
           HRRL+WLSD+LQ+SP ATFTLHRLFGQ Q FTANPAVVQHILKT+FHIYQKGD FRS  +
Sbjct: 61  HRRLEWLSDILQVSPNATFTLHRLFGQAQVFTANPAVVQHILKTQFHIYQKGDPFRSALS 120

Query: 121 DFLGDGIFNADGESWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCV 180
           DFLGDGIFNADGESWKFQRQVSSHEF+TKSLRKFVETVVDAELSDRL P+L+TAA++C V
Sbjct: 121 DFLGDGIFNADGESWKFQRQVSSHEFSTKSLRKFVETVVDAELSDRLTPVLNTAATACSV 180

Query: 181 LDLQDVLQRFAFDNVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWK 240
           LD QDVLQRFAFDNVCKIAFGYDPAYLSPSF QSKFAKAFE+AVRISSLR QSL+P  WK
Sbjct: 181 LDFQDVLQRFAFDNVCKIAFGYDPAYLSPSFGQSKFAKAFEDAVRISSLRFQSLVPIFWK 240

Query: 241 LKKFLNIGSEKQLRIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNF 300
           LKKFL+IGSEK+LRIAVAEVRGYA  II++KK EL+ NSSI +VDLLSRFL+SGHS+ +F
Sbjct: 241 LKKFLDIGSEKRLRIAVAEVRGYAKNIIDEKKNELQTNSSIASVDLLSRFLSSGHSDEDF 300

Query: 301 NIDIIISFILAGQDTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFGYDEVKDLVYT 360
             DIIISFILAGQDTTSAALTW FWLL K+PQVE RI EEI  KSE  FGYDEVK+L YT
Sbjct: 301 VTDIIISFILAGQDTTSAALTWFFWLLPKYPQVEDRILEEICNKSED-FGYDEVKNLTYT 360

Query: 361 HAALCESMRLYPPVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEF 420
           HAALCESMRLYPPVPVDGKQA ADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEF
Sbjct: 361 HAALCESMRLYPPVPVDGKQAVADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEF 420

Query: 421 RPERWLESGGDEAGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFR 480
           RPERWLESG D A   KWRFVGRDNYTYPVFQAGPRICLGKEMAF+QMKR+VAGI+KRFR
Sbjct: 421 RPERWLESGDDAA--AKWRFVGRDNYTYPVFQAGPRICLGKEMAFLQMKRMVAGILKRFR 480

Query: 481 VVPAAEEGVEPRFVQYMTAKMEGGFPVRIKVR 506
           VVPAA EGVEP+FVQYMTAKMEGGFPVRI+ R
Sbjct: 481 VVPAAAEGVEPKFVQYMTAKMEGGFPVRIEAR 506

BLAST of Cucsat.G20714 vs. ExPASy TrEMBL
Match: A0A6J1CX51 (cytochrome P450 94A2-like OS=Momordica charantia OX=3673 GN=LOC111015229 PE=3 SV=1)

HSP 1 Score: 791 bits (2043), Expect = 2.59e-285
Identity = 398/501 (79.44%), Postives = 439/501 (87.62%), Query Frame = 0

Query: 9   FSLF-LLPLLFFAFIFTRTPNPHFTPPIKLPKSYPIVGSFFALFANRHRRLQWLSDVLQI 68
           FSLF L PLLF  F F    N +    ++LPKSYP+VGSFFA+FANRHRRLQWLSD+LQI
Sbjct: 8   FSLFSLFPLLFCIFFFFTKTNKN-PSSVELPKSYPLVGSFFAVFANRHRRLQWLSDLLQI 67

Query: 69  SPAATFTLHRLFGQRQFFTANPAVVQHILKTKFHIYQKGDSFRSIFTDFLGDGIFNADGE 128
           SPAATFTLHRLFGQRQ FTA+PA V HILKT FHIYQKG SFRS  TDFLGDGIFNADG 
Sbjct: 68  SPAATFTLHRLFGQRQVFTADPAAVHHILKTHFHIYQKGHSFRSTLTDFLGDGIFNADGH 127

Query: 129 SWKFQRQVSSHEFTTKSLRKFVETVVDAELSDRLVPILHTAASSCCVLDLQDVLQRFAFD 188
           SWKFQRQVSSHEF+T+SLRKFVE+VVD ELSDRL+PIL +AA++  VLDLQDVLQRFAFD
Sbjct: 128 SWKFQRQVSSHEFSTRSLRKFVESVVDVELSDRLLPILRSAATAPSVLDLQDVLQRFAFD 187

Query: 189 NVCKIAFGYDPAYLSPSFVQSKFAKAFEEAVRISSLRIQSLIPNVWKLKKFLNIGSEKQL 248
           NVCKIAFGYDPAYLSPSF QSKFA AFE+AVRISS R  SL+P VWK+KKFL+IGSEK+L
Sbjct: 188 NVCKIAFGYDPAYLSPSFGQSKFAMAFEDAVRISSGRFASLLPIVWKVKKFLDIGSEKRL 247

Query: 249 RIAVAEVRGYANKIINDKKAELKANSSIDAVDLLSRFLTSGHSNHNFNIDIIISFILAGQ 308
           RIA++EVRGYA  II +KK E+K N+SID+VDLLSRFL+SGHS+ NF  DI+ISFILAG+
Sbjct: 248 RIAISEVRGYAKNIIEEKKNEIKTNASIDSVDLLSRFLSSGHSDDNFVADIVISFILAGR 307

Query: 309 DTTSAALTWLFWLLAKHPQVETRIFEEISQKSEHLFG-YDEVKDLVYTHAALCESMRLYP 368
           DTTSAALTW FWLL+K+P+VE RI +EI+ KSE   G YDEVKDLVYTHAALCESMRLYP
Sbjct: 308 DTTSAALTWFFWLLSKYPEVEDRILDEINDKSEISGGGYDEVKDLVYTHAALCESMRLYP 367

Query: 369 PVPVDGKQAAADDVLPDGTVVRKGERVAYHPYAMGRMEGIWGKDWAEFRPERWLESGGDE 428
           PVPVDGK+AA DDVLPDGT VRKGERVAYHPYAMGR+E IWG DWAEFRPERWLE     
Sbjct: 368 PVPVDGKEAAEDDVLPDGTAVRKGERVAYHPYAMGRLESIWGSDWAEFRPERWLE----R 427

Query: 429 AGAVKWRFVGRDNYTYPVFQAGPRICLGKEMAFMQMKRIVAGIVKRFRVVPAAEEGVEPR 488
            G  KWRFVGRDNYTYPVFQAGPRICLGKEMAF+QMKR+VAGI+KRFRVVPAAEEG EP+
Sbjct: 428 DGPAKWRFVGRDNYTYPVFQAGPRICLGKEMAFLQMKRMVAGILKRFRVVPAAEEGGEPK 487

Query: 489 FVQYMTAKMEGGFPVRIKVRE 507
           FVQYMTAKMEGGFPVRI+ RE
Sbjct: 488 FVQYMTAKMEGGFPVRIEERE 503