Cucsat.G18286 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G18286
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	ENTH domain-containing protein
Location	ctg3345: 4720255 .. 4722321 (-)
RNA-Seq Expression	Cucsat.G18286
Synteny	Cucsat.G18286

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAATCCACCAAAGTTTACACAAGTGGTGAGAAGGGAGTTGGGGAGTGATTGTCAACAATCCATTTATTAAAGTTTCAAAGCCGAAAGGATCGCCGCTCATCAGTCTTAAAATGTGCTAAGCACTTTGACTTTTACACTCAATACATTTCCATAAATTCAACTTTTCATACCAAATCTCATACTTTCACCATTTCCAGATGCCTTCTTTCTTCGTGTGAAAATAAGCTACCCAAAATTTCGTCTTTTTCTTTCTTTCATTCTCTCATCTTTGTTTGTGAAACATCAAATATCATGTCTTCCATCTTGAGCAAATCCAACACCATGCCCACTCCCTCTTTCCATGAATTGAAGAAACAAGCTTCTTTCTTTTTCAAGGAGAAAATCAAGACTGCTAGATTAGCCCTCACAGATGTTACCTCTGCCGAACTGTAAGTTCATTAATTTTTCCTTATGTATTACTAAGATGTCCTGAATATGTTTATCTTATTAGATTATTTAATTATTTACAATTTATTTACGATTATGATCCTTCAAATCTTTTTTGTTTTTGTTCTTGTCTCTGCTTTTTAGGTTGACTGAAGAAGCCATCAGTGGGAACCCTGATGCAAGATCTTTGAGTTCAATATCAAAGGCAGCTTTTGAGGTTGATGACTACTGGAGAATTGTAGCTATTCTCCATAAAAGGTCTTAGACTCTATTTCTTTCATCAATGTTTTGAAGTTTTTTATGTTTCTATTTCAATATAAATTGGACCTGTTTGTCATTTGGATAAAACCCATCATTGATAAAGTTATAGATTCAAAGGGTACAAAGGCTATACTATCTTTTTTAGTCACATTAGACAACGATTGATGGAAACTATATTGATTAGTTTTCAAAATTTGAGTTGGTTTTCTAAACATGTAGAAATCGGTAATATCAAATAGGCTCGGTTCCGTAACATTGTTCGATTTGACAGATAGGTGAAAACACAAACATACTTTATTTCATGATTGTTTAAGTAATAGCAATGAGATTCTCCAAAATCATGAAATAAAGCATTGAATAAGATCTCAGTTTTTTCTTTCTTTTTCTTTAAAAATTTAGTTTTGATTTTGCTGATGGGATCCTTGATTGATAAAAGTAAACAAACTTGCAAGGCAGATTGCTGAAATTTGAAAAGAAGAATTGGAGACTTTCCTACAATTCTCTCATCATTCTCGAACATCTCTTAACTCGTGGACCAGAGAGTGTAGCAGAAGAGTTTCAAACTGACAAAGATGTCATTAACCAAATGGGATCCTTCCAATATGTTGATGAAAAGGGGTAATGCCCGTGTTCGTGTTTTCGTCGAAAACCTATTTTTCTTGAATTTTATTCATAATTTAATGCTGAATTGGTTTTAGGTTCAATTGGGGGATATCAGTGAGGAAGAGATCAGAGAGGATATTGAATCTCCTTGACAAAGGGCCTGTTCTCAAAGAAGAGAGGGAGAAGGCAAGAAAGTTGACGAGAGAAATTCTAGGATTTGGAAGCTTCTCCTTGAGAACAAAGTGTCAAGAAATTGTGGAAGAATCTTCTTCTTCACCCATTGGAAGATATGGAAAATGCAATTCAAACTTTAATTGTCTTGAGAATCTTGACCAAGAAGAACATTTCGTTTTGTTAGAACAAAAGGAAGTGATTTCAGTTAATGATTATCATCATCCTTTCATCAGCAACGAATCGAAGTCGAATGCTTCATTGCTGTTAGGATAATGACAGAGGAATTTGGAATTGTTTCTTTTGAGGTCCATTCATGATTCAGTATCGTATTTACATCTGCTTGAATAGCACATAAAAAGAAAATAAAACATTGCTACCAATGAAAGGAAACGGGTTTTTTATTTTTATTTTTATGTTTTATTTAATGATGAAGAGTATGAAAATTAGCAGTTGTTGGTTTTGTTTGGCTTGCAGAATTCAAAATGAAAAGGCTAAAAGTTTAGCATCTATCTGTATAAAGATTAGTACTCAGTGAGAAACTTTCATATTTTGTTGAAACTGTTATTTCCGTCATTCCAATTATAAGAGCATTTTCTTACAG

Coding sequence (CDS)

ATGGGATCCTTCCAATATGTTGATGAAAAGGGGTTCAATTGGGGGATATCAGTGAGGAAGAGATCAGAGAGGATATTGAATCTCCTTGACAAAGGGCCTGTTCTCAAAGAAGAGAGGGAGAAGGCAAGAAAGTTGACGAGAGAAATTCTAGGATTTGGAAGCTTCTCCTTGAGAACAAAGTGTCAAGAAATTGTGGAAGAATCTTCTTCTTCACCCATTGGAAGATATGGAAAATGCAATTCAAACTTTAATTGTCTTGAGAATCTTGACCAAGAAGAACATTTCGTTTTGTTAGAACAAAAGGAAGTGATTTCAGTTAATGATTATCATCATCCTTTCATCAGCAACGAATCGAAGTCGAATGCTTCATTGCTGTTAGGATAA

Protein sequence

MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTKCQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQKEVISVNDYHHPFISNESKSNASLLLG

Homology

[Close]

BLAST of Cucsat.G18286 vs. ExPASy Swiss-Prot
Match: Q8VY07 (Clathrin interactor EPSIN 1 OS=Arabidopsis thaliana OX=3702 GN=EPSIN1 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.2e-04
Identity = 30/91 (32.97%), Postives = 49/91 (53.85%), Query Frame = 0


Query: 3   SFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTKCQ 62
           SF+YV+  G + GI+VRK++E I+ LL+    + E R+KA     + +G  S  +  K  
Sbjct: 116 SFEYVEPNGKDVGINVRKKAENIVALLNNKEKISEIRDKAVANRNKYVGLSSTGITYK-- 175

Query: 63  EIVEESSSSPIGRYGKCNSNFNCLENLDQEE 94
                SS+S  G +   +SNF+  ++ D  E
Sbjct: 176 ---SGSSASFGGSFQSGSSNFDSYKDRDSRE 201

[Close]

BLAST of Cucsat.G18286 vs. NCBI nr
Match: XP_004140497.1 (clathrin interactor 1 [Cucumis sativus] >KAE8646791.1 hypothetical protein Csa_005646 [Cucumis sativus])

HSP 1 Score: 255 bits (651), Expect = 1.14e-83
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0


Query: 1   MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 60
           MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK
Sbjct: 128 MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 187

Query: 61  CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQKEVISVNDYHHPFISNESKS 120
           CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQKEVISVNDYHHPFISNESKS
Sbjct: 188 CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQKEVISVNDYHHPFISNESKS 247

Query: 121 NASLLLG 127
           NASLLLG
Sbjct: 248 NASLLLG 254

[Close]

BLAST of Cucsat.G18286 vs. NCBI nr
Match: KAA0039750.1 (ENTH domain-containing protein-like protein [Cucumis melo var. makuwa] >TYK24747.1 ENTH domain-containing protein-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 234 bits (598), Expect = 9.39e-76
Identity = 118/127 (92.91%), Postives = 122/127 (96.06%), Query Frame = 0


Query: 1   MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 60
           MGSFQYVDEKGFNWGISVRKRSERIL+LLDK PVLKEEREKARKLTREILGFGSFSLR+K
Sbjct: 118 MGSFQYVDEKGFNWGISVRKRSERILDLLDKRPVLKEEREKARKLTREILGFGSFSLRSK 177

Query: 61  CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQKEVISVNDYHHPFISNESKS 120
           CQEIVE SSSSPIGRYGKCNSNFN LENL+QEEH VLLEQKEVISVNDYHHPFI +ESKS
Sbjct: 178 CQEIVEHSSSSPIGRYGKCNSNFNSLENLEQEEHLVLLEQKEVISVNDYHHPFIIDESKS 237

Query: 121 NASLLLG 127
           NASLLLG
Sbjct: 238 NASLLLG 244

[Close]

BLAST of Cucsat.G18286 vs. NCBI nr
Match: XP_008459736.1 (PREDICTED: ENTH domain-containing protein C794.11c-like [Cucumis melo])

HSP 1 Score: 234 bits (598), Expect = 1.25e-75
Identity = 118/127 (92.91%), Postives = 122/127 (96.06%), Query Frame = 0


Query: 1   MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 60
           MGSFQYVDEKGFNWGISVRKRSERIL+LLDK PVLKEEREKARKLTREILGFGSFSLR+K
Sbjct: 127 MGSFQYVDEKGFNWGISVRKRSERILDLLDKRPVLKEEREKARKLTREILGFGSFSLRSK 186

Query: 61  CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQKEVISVNDYHHPFISNESKS 120
           CQEIVE SSSSPIGRYGKCNSNFN LENL+QEEH VLLEQKEVISVNDYHHPFI +ESKS
Sbjct: 187 CQEIVEHSSSSPIGRYGKCNSNFNSLENLEQEEHLVLLEQKEVISVNDYHHPFIIDESKS 246

Query: 121 NASLLLG 127
           NASLLLG
Sbjct: 247 NASLLLG 253

[Close]

BLAST of Cucsat.G18286 vs. NCBI nr
Match: XP_038875427.1 (clathrin interactor 1-like [Benincasa hispida])

HSP 1 Score: 208 bits (530), Expect = 2.74e-65
Identity = 109/129 (84.50%), Postives = 120/129 (93.02%), Query Frame = 0


Query: 1   MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 60
           +G+ QYVDE+GFNWGISVRKRSERILNLLDKG VLKEEREKARKLTR+ILGFGSFSLR+K
Sbjct: 127 IGTLQYVDERGFNWGISVRKRSERILNLLDKGSVLKEEREKARKLTRDILGFGSFSLRSK 186

Query: 61  CQEIVEESSSS-PIGRYGKCNSNFNCLENLDQEEHFVLLEQKE-VISVNDYHHPFISNES 120
           CQEIVE+SSSS PIGRYGKCNSNFN LENL+QEEH VLLEQK+ +IS NDYH PFI++ES
Sbjct: 187 CQEIVEDSSSSSPIGRYGKCNSNFNRLENLEQEEHLVLLEQKDGIISANDYH-PFINDES 246

Query: 121 KSNASLLLG 127
           K NASLLLG
Sbjct: 247 KLNASLLLG 254

[Close]

BLAST of Cucsat.G18286 vs. NCBI nr
Match: XP_038893947.1 (clathrin interactor EPSIN 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 153 bits (387), Expect = 4.09e-43
Identity = 81/100 (81.00%), Postives = 88/100 (88.00%), Query Frame = 0


Query: 1   MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 60
           MG+FQYVDEKGFNWGISVRKRSERILNLLDK  +LK ER+KARKLTREILGFGSFSLR+K
Sbjct: 118 MGNFQYVDEKGFNWGISVRKRSERILNLLDKRSLLKNERDKARKLTREILGFGSFSLRSK 177

Query: 61  CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQ 100
            Q I+E SSS PI RYGKCNSNFN L+NLDQE H VL +Q
Sbjct: 178 SQGILEHSSS-PIARYGKCNSNFNSLDNLDQE-HQVLSDQ 215

[Close]

BLAST of Cucsat.G18286 vs. ExPASy TrEMBL
Match: A0A0A0KDV3 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G108500 PE=4 SV=1)

HSP 1 Score: 255 bits (651), Expect = 3.96e-84
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0


Query: 1   MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 60
           MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK
Sbjct: 118 MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 177

Query: 61  CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQKEVISVNDYHHPFISNESKS 120
           CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQKEVISVNDYHHPFISNESKS
Sbjct: 178 CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQKEVISVNDYHHPFISNESKS 237

Query: 121 NASLLLG 127
           NASLLLG
Sbjct: 238 NASLLLG 244

[Close]

BLAST of Cucsat.G18286 vs. ExPASy TrEMBL
Match: A0A5A7T920 (ENTH domain-containing protein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G003100 PE=4 SV=1)

HSP 1 Score: 234 bits (598), Expect = 4.55e-76
Identity = 118/127 (92.91%), Postives = 122/127 (96.06%), Query Frame = 0


Query: 1   MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 60
           MGSFQYVDEKGFNWGISVRKRSERIL+LLDK PVLKEEREKARKLTREILGFGSFSLR+K
Sbjct: 118 MGSFQYVDEKGFNWGISVRKRSERILDLLDKRPVLKEEREKARKLTREILGFGSFSLRSK 177

Query: 61  CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQKEVISVNDYHHPFISNESKS 120
           CQEIVE SSSSPIGRYGKCNSNFN LENL+QEEH VLLEQKEVISVNDYHHPFI +ESKS
Sbjct: 178 CQEIVEHSSSSPIGRYGKCNSNFNSLENLEQEEHLVLLEQKEVISVNDYHHPFIIDESKS 237

Query: 121 NASLLLG 127
           NASLLLG
Sbjct: 238 NASLLLG 244

[Close]

BLAST of Cucsat.G18286 vs. ExPASy TrEMBL
Match: A0A1S3CAD9 (ENTH domain-containing protein C794.11c-like OS=Cucumis melo OX=3656 GN=LOC103498779 PE=4 SV=1)

HSP 1 Score: 234 bits (598), Expect = 6.07e-76
Identity = 118/127 (92.91%), Postives = 122/127 (96.06%), Query Frame = 0


Query: 1   MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 60
           MGSFQYVDEKGFNWGISVRKRSERIL+LLDK PVLKEEREKARKLTREILGFGSFSLR+K
Sbjct: 127 MGSFQYVDEKGFNWGISVRKRSERILDLLDKRPVLKEEREKARKLTREILGFGSFSLRSK 186

Query: 61  CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQKEVISVNDYHHPFISNESKS 120
           CQEIVE SSSSPIGRYGKCNSNFN LENL+QEEH VLLEQKEVISVNDYHHPFI +ESKS
Sbjct: 187 CQEIVEHSSSSPIGRYGKCNSNFNSLENLEQEEHLVLLEQKEVISVNDYHHPFIIDESKS 246

Query: 121 NASLLLG 127
           NASLLLG
Sbjct: 247 NASLLLG 253

[Close]

BLAST of Cucsat.G18286 vs. ExPASy TrEMBL
Match: A0A6J1DDE5 (clathrin interactor EPSIN 1 OS=Momordica charantia OX=3673 GN=LOC111019473 PE=4 SV=1)

HSP 1 Score: 149 bits (376), Expect = 1.24e-41
Identity = 79/101 (78.22%), Postives = 86/101 (85.15%), Query Frame = 0


Query: 1   MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 60
           MG FQYVDEKGFNWGISVRKRSERIL+LL+KG +LKEEREKARKLTREILGFGSFSLR+K
Sbjct: 128 MGRFQYVDEKGFNWGISVRKRSERILSLLEKGSLLKEEREKARKLTREILGFGSFSLRSK 187

Query: 61  CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFVLLEQK 101
              I+ ESS S IGRYGKCNSN N  +NLDQE   VL +QK
Sbjct: 188 SPGILVESSQS-IGRYGKCNSNSNIQDNLDQEHDRVLSDQK 227

[Close]

BLAST of Cucsat.G18286 vs. ExPASy TrEMBL
Match: A0A0A0LVB4 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G507460 PE=4 SV=1)

HSP 1 Score: 143 bits (361), Expect = 1.71e-39
Identity = 74/114 (64.91%), Postives = 88/114 (77.19%), Query Frame = 0


Query: 1   MGSFQYVDEKGFNWGISVRKRSERILNLLDKGPVLKEEREKARKLTREILGFGSFSLRTK 60
           M +FQYVDEKGFNWG+SVRKRSERILNLLDKG +LK EREKARKLTREILGFGSFSLR+ 
Sbjct: 118 MANFQYVDEKGFNWGLSVRKRSERILNLLDKGSLLKNEREKARKLTREILGFGSFSLRSN 177

Query: 61  CQEIVEESSSSPIGRYGKCNSNFNCLENLDQEEHFV-------LLEQKEVISVN 107
            Q I+ +  SSPI RYGKCNSNF+ L+N+  ++  V       +LE +E +  N
Sbjct: 178 SQGIILQHPSSPIARYGKCNSNFDSLDNILHQDGSVVDGQRIEMLETRESVDEN 231

The following BLAST results are available for this feature:

BLAST of Cucsat.G18286 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VY07	4.2e-04	32.97	Clathrin interactor EPSIN 1 OS=Arabidopsis thaliana OX=3702 GN=EPSIN1 PE=1 SV=1	[more]

BLAST of Cucsat.G18286 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140497.1	1.14e-83	100.00	clathrin interactor 1 [Cucumis sativus] >KAE8646791.1 hypothetical protein Csa_0...	[more]
KAA0039750.1	9.39e-76	92.91	ENTH domain-containing protein-like protein [Cucumis melo var. makuwa] >TYK24747...	[more]
XP_008459736.1	1.25e-75	92.91	PREDICTED: ENTH domain-containing protein C794.11c-like [Cucumis melo]	[more]
XP_038875427.1	2.74e-65	84.50	clathrin interactor 1-like [Benincasa hispida]	[more]
XP_038893947.1	4.09e-43	81.00	clathrin interactor EPSIN 1 isoform X1 [Benincasa hispida]	[more]

BLAST of Cucsat.G18286 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KDV3	3.96e-84	100.00	ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G108500 PE=4 S...	[more]
A0A5A7T920	4.55e-76	92.91	ENTH domain-containing protein-like protein OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3CAD9	6.07e-76	92.91	ENTH domain-containing protein C794.11c-like OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A6J1DDE5	1.24e-41	78.22	clathrin interactor EPSIN 1 OS=Momordica charantia OX=3673 GN=LOC111019473 PE=4 ...	[more]
A0A0A0LVB4	1.71e-39	64.91	ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G507460 PE=4 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008942	ENTH/VHS	GENE3D	1.25.40.90		coord: 1..55 e-value: 1.4E-10 score: 43.2
IPR008942	ENTH/VHS	SUPERFAMILY	48464	ENTH/VHS domain	coord: 3..53
None	No IPR available	PANTHER	PTHR12276:SF99	EPSIN-2-LIKE	coord: 1..125
None	No IPR available	PANTHER	PTHR12276	EPSIN/ENT-RELATED	coord: 1..125
IPR013809	ENTH domain	PROSITE	PS50942	ENTH	coord: 1..39 score: 10.768232

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G18286.T1	Cucsat.G18286.T1	mRNA
Cucsat.G18286.T2	Cucsat.G18286.T2	mRNA
Cucsat.G18286.T3	Cucsat.G18286.T3	mRNA
Cucsat.G18286.T5	Cucsat.G18286.T5	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006897	endocytosis
cellular_component	GO:0030125	clathrin vesicle coat
cellular_component	GO:0005768	endosome
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0030276	clathrin binding
molecular_function	GO:0005543	phospholipid binding