Cucsat.G18270 (gene) Cucumber (B10) v3

Overview
NameCucsat.G18270
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionSAM domain-containing protein
Locationctg3345: 4329904 .. 4332482 (-)
RNA-Seq ExpressionCucsat.G18270
SyntenyCucsat.G18270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAATGAAAGAGAGGGAGAGGGAGAGGGAGAGAAAAAAGGAATAGTATATAGTATTTGGTGAGTGAGGAAAAATAAAGAAGAAGCGTGTGGTAGACGAGAAATAAGAAGCGCGTGTGGAATGGGAGTTTTAGAGACAGTAAGGCAAAGCATAGAAGTGCGAGGGAATGGAATTGAGGAAAGAGAGAAAAAAAGAAAAGAAAAGAAATAATGAAATGATCAAAACCCTTCTCTCATTTCTTCTTCCTTCCATTCTTCCTTCCTTCCTTCTTCAACTCTCACCTCTCACCTCTCTACTCCCAACTTTCAACTCTCAAACATCATCAACAACAACAACAGCAACAACGCTCCTCTCCCTCTCTTTGTTTCAACTTCTCTTTCTCAATCAAATGTAGAGAAATTTCCTCTGACCTACCTTTTCTTCTCAACAACAAGGTGAATTTCAACTTCTCTCTCACTCACTTCCATCAATTTCTTCTCTATCACCTTAATTTCTGATGCGTTTTCTTTTCAATTTTTAGGCTTTCTGAAATTCCGTTCCACCTCATAGGGAGATGAACAAGGAACAGCGACCTGAACCTCTTGATTTCTTCATTTGGACTGTTGAGGTATTTCCAAATCATACCTCCTCCTTCCTTTTCGATCCTTTTCTGCTAAGATCTTTGACTCCTTTGCCTTTCTACTCCAAAATCTGATTGGTTCTGTGTTAATTACAGTGTTGGTTTCTGAGGTTTGAGATTGATGCCTATTCACTACTCTACCCTTTCTCATTCATTGCTTTTTCCAGTATTTGATAGTCAATACAGTGATTTCTTTTGTTTTTTTCTCTTCTGTTTATTAATGGTAACGTGTGACTACTGGATCAATGAGGTTTGATTGGTGTCTAATTTCTTTTTTTCTAATTGTGTATGTTATGATTAGGATGTTGGTGTTTCGTTAGCCACTGAAGACTTTTCTAACTAATTGTTAATAGTGAAATGACATAAATGAATATATTGATAGTCCTTAATGTAGGTAGGAGCTTGATTCTAAAATATCACTTTCTGGAATTCATTTACACACGCAAAGATCTGTTTATTGCACTAGGATTTGTTTTCCTTCCTTTTTGGAATTTTTTACTTTTCCTTGTTGTAGTTCCATTTCATCTTCTTTATTATTTCCTTTGTGAACTTTGTAATTACCTGGAGCTCTCGAAAATTTGATTCCTAGAAATCTTGAATAAGAAATTATTTTTGGTGGAACTGAAAATTTTACATTAGCAATGGCATAGTGCTTCTATGTTCCGTTTTCAGTATGCTTTTAGATGTCACATAATTTCTTAGCTTGTACAAATGCAATAATGTGTGAAGATATCATTTTAAACTTCGCCCTGTCCTTTCAAGTTGGAGTGGTTATGTGTGCATTGCTTAGCTTGATCCTTGTGTAATCTTCTGAAACCGTATCATCATTCTTATTATACGGGGTTAGAAAAGATATCCTTACATGGAAAAAGATTTTTTCAGCATTTGGACTATACATTATATCACATCTCTCGGGTCAAAATTTTAACAGTAAATGTAACTAAATTTGTTTTATAATGTGCCTCCTGCATGCTTATTATAACGCCTTTTCCCTTTAGGATGTTGGTTTGTGGTTGGAAGAGATAAATCTTGGAGGCTATCGCCAGATTTTTAAAGAAAACGGGGTCAATGGTGAGTATCTTGAAGGGATGTCAATGTTCACAACTGAACAGATTCTAAGGTTTATCAGACGATGCCATATGAAATGGGGAGATTTCATTACACTTTGTAAGGAACTTCGACGAATTAAAGGTCTGTGTGCAAAGCAACATTTCTTCTCTTTTGAATTTAATTTTATTAGGAAAAAGAACTCCAGAGGTGTCTTACTGCTATATTTAGATATTTCAGAAAGTCTTACATTTGGTTTGAAATTAATTAAATTCTGAACAGAACAAAATCCACCAAATCTTAACTAAATGCATGCAAATTAGCATATTTTACAACAGAGTAGGAGTAAGCCTTTATATGGGACTTTCTTCAATGTCTCTTACCTTTTTTTTTCCCTCTGGTTTAAGCAGTCTGTGTTCGTCTTTAAAAGTTCGGTGCTGTACATCAACTTTTGTCTCCTTATTTGATGTATTTGCAATCTCTTGTTGTTATTAGCATGTTTCTGATAGTCCTAGTTGAAAAATTGATGCACGCACTGACTGTTATTCTAAGAGGGGGTTTGAGATAACTTTTGGGGCAGCCACCCTACATCTTTTTCCTCCTTTTCCCCCTTTTCTCCCTTTATATTAGAAGCAACATTAGACTAGTACCTTTTTTGCCTTTTTTGACAGAAGTGAAAGAATGTACGATTCCATACAGGAACACAAGTCCATAAAAAGAGAATTTCAAATGTTACAAACGAAATAGACCCCAAACCAAATCATTAGGCTATTTTAAGGTCGGTCCTTTTAAGTACTCTTAGAACTTTCAAAACAACATTTAACTTTGTTTTTAATTCCAATGCAGTAACTTGATGTATATGTCTGCTTAACTTGGCTTTTGAAACATTAGAACTTTCAGAATTGCCATTCCAAAAGCAACCCCAACTATGTATGTCTGCTTTTCCAGTTGACATCAAGATCATGCTGTTTATTCTTTAAGTTATGTACTTCCAGTGGCTTGCTTGAAAGGGGAGCAAAAGGTACGCCGGCCATGGTGGGCACCATCGTGCCTCTCTATGGTGTTTCTCAAGGTGGCTAAGCGTAACAGACAATCTCGAGTTGTTTCCTTGAAGCTGGAACCATAGAACTGAGATCAACTTATTTGTATGTGCTCATGTATTTCTTTTTATGCGTGAAGAATATAAAAGGTAGAGAAGAAACATATTAGTTTATCCTTTTCTAAAAAAATTTAATCGGTCAATATTACCTTCTCTTTTTTGTCCTTTGTTTAGAACTATGTCTATATATACAAGGTTTATGTGTTCAGTTTGGCGATCTCGCTTGAGGAAAAACAATGAAGCAACCAAGATCTTGTTGCAAATTATACAAGTTTTGCTCTTTTACCCTCACTAACTCTTCAAATATGTATCTACTTTCAACATTCAATTTCGTAATGACTTTGGTTTAGGAAATTATGCCTGTATATAGTCGAATCATGTTTTGTCCTCTAATTTTGGGCAGTCCTTTCTTAAGATGGGTGTTTCTAATTATGGTATAGAATAGTGGGAGAAGCTTGCGAGTTTCGGAGCTTGCGTTGGACACTACTAATTGTTTGTACATTGAATTGATAGTGAAAAGGATACATTTAATATCTGTGGCACTCAAATCCACAAACCCACATGGAAGACAGAGGACATTATTCCATCATCTTCTTCCAAGAACTGCAAAGTAGAAAGTTTCCATTAAAATCCTACCAAGATCTCACCCTTTCTATCCCATTTCTTTTTATTTCCCATTGTTTCAGGTGAAGTTTCTTTCCTCTTCTTCCATGGAGCTGAAAGGTTGGTTGGTAATTCAGTTTTCTTCTTAT

Coding sequence (CDS)

ATGAACAAGGAACAGCGACCTGAACCTCTTGATTTCTTCATTTGGACTGTTGAGGATGTTGGTTTGTGGTTGGAAGAGATAAATCTTGGAGGCTATCGCCAGATTTTTAAAGAAAACGGGGTCAATGGTGAGTATCTTGAAGGGATGTCAATGTTCACAACTGAACAGATTCTAAGGTTTATCAGACGATGCCATATGAAATGGGGAGATTTCATTACACTTTGTAAGGAACTTCGACGAATTAAAGAACTTTCAGAATTGCCATTCCAAAAGCAACCCCAACTATGTATGTCTGCTTTTCCAGTTGACATCAAGATCATGCTGTTTATTCTTTAA

Protein sequence

MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRFIRRCHMKWGDFITLCKELRRIKELSELPFQKQPQLCMSAFPVDIKIMLFIL
Homology
[Close]
BLAST of Cucsat.G18270 vs. ExPASy Swiss-Prot
Match: P18160 (Dual specificity protein kinase splA OS=Dictyostelium discoideum OX=44689 GN=splA PE=1 SV=3)

HSP 1 Score: 46.2 bits (108), Expect = 2.6e-04
Identity = 16/36 (44.44%), Postives = 25/36 (69.44%), Query Frame = 0 

Query: 15   WTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMS 51
            W   DV +WLE  N G YR+ F++N ++G +LEG++
Sbjct: 1734 WAPNDVAIWLESFNYGQYRKNFRDNNISGRHLEGIT 1769

[Close]
BLAST of Cucsat.G18270 vs. NCBI nr
Match: XP_031743876.1 (uncharacterized protein LOC101220276 isoform X2 [Cucumis sativus])

HSP 1 Score: 178 bits (452), Expect = 8.37e-56
Identity = 82/82 (100.00%), Postives = 82/82 (100.00%), Query Frame = 0 

Query: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60
          MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF
Sbjct: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60

Query: 61 IRRCHMKWGDFITLCKELRRIK 82
          IRRCHMKWGDFITLCKELRRIK
Sbjct: 61 IRRCHMKWGDFITLCKELRRIK 82

[Close]
BLAST of Cucsat.G18270 vs. NCBI nr
Match: XP_004140005.1 (uncharacterized protein LOC101220276 isoform X1 [Cucumis sativus] >KGN46598.1 hypothetical protein Csa_005059 [Cucumis sativus])

HSP 1 Score: 178 bits (452), Expect = 1.31e-55
Identity = 82/82 (100.00%), Postives = 82/82 (100.00%), Query Frame = 0 

Query: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60
          MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF
Sbjct: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60

Query: 61 IRRCHMKWGDFITLCKELRRIK 82
          IRRCHMKWGDFITLCKELRRIK
Sbjct: 61 IRRCHMKWGDFITLCKELRRIK 82

[Close]
BLAST of Cucsat.G18270 vs. NCBI nr
Match: KAG7010778.1 (hypothetical protein SDJN02_27574 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 176 bits (445), Expect = 5.57e-55
Identity = 80/83 (96.39%), Postives = 82/83 (98.80%), Query Frame = 0 

Query: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60
          MNK+QRPEPLDFFIWTVEDVGLWLEEI+LG YRQIFKENGVNGEYLEGMSMFTTEQILRF
Sbjct: 1  MNKDQRPEPLDFFIWTVEDVGLWLEEISLGSYRQIFKENGVNGEYLEGMSMFTTEQILRF 60

Query: 61 IRRCHMKWGDFITLCKELRRIKG 83
          IRRCHMKWGDFITLCKELRRIKG
Sbjct: 61 IRRCHMKWGDFITLCKELRRIKG 83

[Close]
BLAST of Cucsat.G18270 vs. NCBI nr
Match: NP_001284396.1 (uncharacterized LOC103502119 [Cucumis melo] >XP_008464174.1 PREDICTED: uncharacterized LOC103502119 isoform X1 [Cucumis melo] >KAA0046055.1 hypothetical protein E6C27_scaffold243G006330 [Cucumis melo var. makuwa] >TYK11934.1 hypothetical protein E5676_scaffold177G001110 [Cucumis melo var. makuwa] >BAG55245.1 downward leaf curling protein [Cucumis melo])

HSP 1 Score: 176 bits (447), Expect = 7.56e-55
Identity = 81/82 (98.78%), Postives = 81/82 (98.78%), Query Frame = 0 

Query: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60
          MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQ FKENGVNGEYLEGMSMFTTEQILRF
Sbjct: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQTFKENGVNGEYLEGMSMFTTEQILRF 60

Query: 61 IRRCHMKWGDFITLCKELRRIK 82
          IRRCHMKWGDFITLCKELRRIK
Sbjct: 61 IRRCHMKWGDFITLCKELRRIK 82

[Close]
BLAST of Cucsat.G18270 vs. NCBI nr
Match: XP_022140752.1 (uncharacterized protein LOC111011338 [Momordica charantia] >XP_022140753.1 uncharacterized protein LOC111011338 [Momordica charantia] >XP_038900530.1 uncharacterized protein LOC120087724 [Benincasa hispida])

HSP 1 Score: 176 bits (446), Expect = 1.07e-54
Identity = 81/82 (98.78%), Postives = 81/82 (98.78%), Query Frame = 0 

Query: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60
          MNKEQRPEPLDFFIWTVEDVGLWLEEINLG YRQIFKENGVNGEYLEGMSMFTTEQILRF
Sbjct: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGSYRQIFKENGVNGEYLEGMSMFTTEQILRF 60

Query: 61 IRRCHMKWGDFITLCKELRRIK 82
          IRRCHMKWGDFITLCKELRRIK
Sbjct: 61 IRRCHMKWGDFITLCKELRRIK 82

[Close]
BLAST of Cucsat.G18270 vs. ExPASy TrEMBL
Match: A0A0A0KAT3 (SAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G111390 PE=4 SV=1)

HSP 1 Score: 178 bits (452), Expect = 6.33e-56
Identity = 82/82 (100.00%), Postives = 82/82 (100.00%), Query Frame = 0 

Query: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60
          MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF
Sbjct: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60

Query: 61 IRRCHMKWGDFITLCKELRRIK 82
          IRRCHMKWGDFITLCKELRRIK
Sbjct: 61 IRRCHMKWGDFITLCKELRRIK 82

[Close]
BLAST of Cucsat.G18270 vs. ExPASy TrEMBL
Match: B3XZN9 (Downward leaf curling protein OS=Cucumis melo OX=3656 GN=CmDLC PE=2 SV=1)

HSP 1 Score: 176 bits (447), Expect = 3.66e-55
Identity = 81/82 (98.78%), Postives = 81/82 (98.78%), Query Frame = 0 

Query: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60
          MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQ FKENGVNGEYLEGMSMFTTEQILRF
Sbjct: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQTFKENGVNGEYLEGMSMFTTEQILRF 60

Query: 61 IRRCHMKWGDFITLCKELRRIK 82
          IRRCHMKWGDFITLCKELRRIK
Sbjct: 61 IRRCHMKWGDFITLCKELRRIK 82

[Close]
BLAST of Cucsat.G18270 vs. ExPASy TrEMBL
Match: A0A5D3CL86 (SAM domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold177G001110 PE=4 SV=1)

HSP 1 Score: 176 bits (447), Expect = 3.66e-55
Identity = 81/82 (98.78%), Postives = 81/82 (98.78%), Query Frame = 0 

Query: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60
          MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQ FKENGVNGEYLEGMSMFTTEQILRF
Sbjct: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQTFKENGVNGEYLEGMSMFTTEQILRF 60

Query: 61 IRRCHMKWGDFITLCKELRRIK 82
          IRRCHMKWGDFITLCKELRRIK
Sbjct: 61 IRRCHMKWGDFITLCKELRRIK 82

[Close]
BLAST of Cucsat.G18270 vs. ExPASy TrEMBL
Match: A0A6J1CHY9 (uncharacterized protein LOC111011338 OS=Momordica charantia OX=3673 GN=LOC111011338 PE=4 SV=1)

HSP 1 Score: 176 bits (446), Expect = 5.20e-55
Identity = 81/82 (98.78%), Postives = 81/82 (98.78%), Query Frame = 0 

Query: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60
          MNKEQRPEPLDFFIWTVEDVGLWLEEINLG YRQIFKENGVNGEYLEGMSMFTTEQILRF
Sbjct: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGSYRQIFKENGVNGEYLEGMSMFTTEQILRF 60

Query: 61 IRRCHMKWGDFITLCKELRRIK 82
          IRRCHMKWGDFITLCKELRRIK
Sbjct: 61 IRRCHMKWGDFITLCKELRRIK 82

[Close]
BLAST of Cucsat.G18270 vs. ExPASy TrEMBL
Match: A0A6J1I147 (uncharacterized protein LOC111469897 OS=Cucurbita maxima OX=3661 GN=LOC111469897 PE=4 SV=1)

HSP 1 Score: 176 bits (445), Expect = 7.38e-55
Identity = 80/82 (97.56%), Postives = 81/82 (98.78%), Query Frame = 0 

Query: 1  MNKEQRPEPLDFFIWTVEDVGLWLEEINLGGYRQIFKENGVNGEYLEGMSMFTTEQILRF 60
          MNKEQRPEPLDFFIWT+EDVGLWLEEINLG YRQIFKENGVNGEYLEGMSMFTTEQILRF
Sbjct: 1  MNKEQRPEPLDFFIWTIEDVGLWLEEINLGSYRQIFKENGVNGEYLEGMSMFTTEQILRF 60

Query: 61 IRRCHMKWGDFITLCKELRRIK 82
          IRRCHMKWGDFITLCKELRRIK
Sbjct: 61 IRRCHMKWGDFITLCKELRRIK 82

The following BLAST results are available for this feature:
BLAST of Cucsat.G18270 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P181602.6e-0444.44Dual specificity protein kinase splA OS=Dictyostelium discoideum OX=44689 GN=spl... [more]
BLAST of Cucsat.G18270 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_031743876.18.37e-56100.00uncharacterized protein LOC101220276 isoform X2 [Cucumis sativus][more]
XP_004140005.11.31e-55100.00uncharacterized protein LOC101220276 isoform X1 [Cucumis sativus] >KGN46598.1 hy... [more]
KAG7010778.15.57e-5596.39hypothetical protein SDJN02_27574 [Cucurbita argyrosperma subsp. argyrosperma][more]
NP_001284396.17.56e-5598.78uncharacterized LOC103502119 [Cucumis melo] >XP_008464174.1 PREDICTED: uncharact... [more]
XP_022140752.11.07e-5498.78uncharacterized protein LOC111011338 [Momordica charantia] >XP_022140753.1 uncha... [more]
BLAST of Cucsat.G18270 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KAT36.33e-56100.00SAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G111390 PE=4 SV... [more]
B3XZN93.66e-5598.78Downward leaf curling protein OS=Cucumis melo OX=3656 GN=CmDLC PE=2 SV=1[more]
A0A5D3CL863.66e-5598.78SAM domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A6J1CHY95.20e-5598.78uncharacterized protein LOC111011338 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A6J1I1477.38e-5597.56uncharacterized protein LOC111469897 OS=Cucurbita maxima OX=3661 GN=LOC111469897... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013761Sterile alpha motif/pointed domain superfamilyGENE3D1.10.150.50coord: 14..85
e-value: 2.9E-9
score: 39.2
IPR013761Sterile alpha motif/pointed domain superfamilySUPERFAMILY47769SAM/Pointed domaincoord: 7..85
IPR001660Sterile alpha motif domainPFAMPF07647SAM_2coord: 15..51
e-value: 2.4E-7
score: 30.8
IPR001660Sterile alpha motif domainPROSITEPS50105SAM_DOMAINcoord: 15..50
score: 11.070639
NoneNo IPR availablePANTHERPTHR47626STERILE ALPHA MOTIF (SAM) DOMAIN-CONTAINING PROTEINcoord: 1..125
NoneNo IPR availablePANTHERPTHR47626:SF4SUBFAMILY NOT NAMEDcoord: 1..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G18270.T4Cucsat.G18270.T4mRNA
Cucsat.G18270.T2Cucsat.G18270.T2mRNA
Cucsat.G18270.T1Cucsat.G18270.T1mRNA
Cucsat.G18270.T14Cucsat.G18270.T14mRNA
Cucsat.G18270.T11Cucsat.G18270.T11mRNA
Cucsat.G18270.T17Cucsat.G18270.T17mRNA
Cucsat.G18270.T16Cucsat.G18270.T16mRNA
Cucsat.G18270.T15Cucsat.G18270.T15mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity
molecular_function GO:0005515 protein binding