Cucsat.G17487 (gene) Cucumber (B10) v3

Overview
NameCucsat.G17487
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionRhodanese domain-containing protein
Locationctg278: 137650 .. 142919 (-)
RNA-Seq ExpressionCucsat.G17487
SyntenyCucsat.G17487
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACGGAGAAAGAACGAAGAACGCGAACGACTCGTCGTCCTCACCCTCCATGCTCATCAGATATAGAGCTCCTCCATTGTTTCCTCTGGGAATGGTATCGCCATTTCCTTCTTCTTCCTCCTCCTCCTTTTCTTCCCTTTCTTCCTTTTCTTCAAACTCTAATCTCATCTCATTCAATTTCAGTCCCTCTTTCCCTATTCCAAGACCCTCCCTTTCTTTCCCTTCGCTGCTCTCCGCTTTCCTTCACAAGCCCTGTCGGAGCATGACTCATTCCGTTTGCTTCTCTGGACCTTCCCCCACCGCTCCCATTCATAGCTCATCTGAAGAATCTGAGGATGTGGAATCAGCTTCTCTTGTTGTTGTTTCTTTCTATAAGTTCGCTGATTTTCCTGACCATGCCGAATTTCGCTTGCCCTTGAAGAAGCTCTGCGAGGAACTGGTACTTGTTTTTTCCTGCTTTATGTGTTTCGATTGCTGTTTTATATGGTTTAGTTGTGTTTGGGAGTTGGGGGTTTCTTGATTATGAGGTTATGATGCTGAAGGGTTTTGGGGTTTCATAAGTGGGTTCTTGTTATGAAGTGATACATTTGGAATTTTGGCTCAGTCTTTGGAGTAATGGAACTTGGTGTTGAGATATAAGTTTACCAAATATTCGGTTGTTGATTAGTTAAGAGCAAGCCTGTTGGTAGCTCTTACCTCTGAAGAGAGTAATTACTAATGAGTAAAAGTTTCACTTGCTCATCATAATGTGTTGTGCATGTAGTTGCCTTCCCATTTCTTTGTAACAAGGGCATCTTTCAACGAATTAGAAAGAAATTTTGTTTGTCTTAGAAATTTTCCTCTCCTTCTGATTAGGAACCTACATTGCTCTGGAAGAGCTCGAAAGGCTTATAGTAATAGAAACACGTCAATCAAATATTTTTTATTTCCTTTAATTCATATTTACACAGCAGAAGGAACCAACGAAATGACTGGTCAAGCATCTGGGAGGAAGAGGTATAAAGTTAGGGAGTGATTCATGATCGATCCCTCCTAAAGAAACAATCCAAGAGAAGACCCTGGCATGCTTTGGGATCTTAACCTTCCATAGGTTAGAATAGAAATGGATGGGAACTGGAGATGAGGAAGCAAAGATTAAATCTCGAGCAGCAGAAGTAAAAAGTTTATTGAAAAAAACACTTGCACAAATCTTCTTCAGAAAAAGAAATAAGACTTACATGGGCCTGCGCAAGGAAATTGCATGGCCCCTATGCAAGGATGACATGCACAAAAAAGAAAAAGAAAGAGAAAAAAGACTTGCACAAAAATACTCGGGAAGGATTAAGAGAAAGACAGTGATAATGTAAACCCCAGACGAAGACTCGAGTTAAATCAATGACAGTAGGGAAGTCCACCTCTTTAATGGAAAGGGAAGAGCTTTTATTTATTTATTTATAAAGAATGAATTATTTTGGGCATCCAACAGGAAGTCCTTCGAAAGTAAGGGACTTCAGGAGAAAGCTCCCTGTAGAAATAAAAAGCTGAGGCTTTAGATACTCCCTTGACAAGGACTTGGTTACAGTTTCGCAGCTTTAGATACTCCCTTGACGAGGACTGGGTTACAGTTTGGGACTAGCAAACGAAAGGGATTACCACCAATTCAGTGGTTGTCCCAACCCAGACTTAAAATTGATTTATTTTCCATCGTTTAGGGAAGTAGATAATCAACTATTTCTTATCTGAAAAAGATCAGCAAATATTATAAAATTTATTTGATTGTCATTCTTTAGCAAGAACATATAGGTATTAGCTGGATATATTTAACATATGCCAGTAAGAACTCGAAGTAATTTGCAATATAGAGAACCCTTTTTATTTCATGAAGTTGAAAGATGAACTTGAGTTTTTATTCCACAGTATAAAGAATGAAGTGAATACAAGTTAACCACTAATACTAAGAGAGACTAGATATGAAACATTTTCCAACTTCCAGTTTGTAAACTTCATCAGTTATTATAAATTTATACCTCATTTTGCTTCCCTGTTTGGATTACGGTACTAAGCTAATCTTGCTCAGCGTGTTTCAGGAGGTATCATTCTCGCACCTGAAGGAATTAATGGGAGCATATGTGGATCTCGGGAGTCTGTCCAAAGGGTTCTTGGATTCATCCAAAGTGATGAACGGCTAAAAGGACTAAGGCAGGTGGAAACACCTGTTAGTCCTGAAGAAGAAGCCATCCACAACGGTCATACTAGCAGTTCCCCTCTTGCAGCGGGGGAAGATGCACCCTTTCGTTGGGACCATGTCAGGGTCAAATTAAAGAAAGAGGTACCAAAATTTTAATTTATTTTCTTTATTCTTAAGGATGTGGGAATCGGGATGGAGGGCAGATGTAGAATATAAGGGCTCGGATATGTTTTCTTATTTGTTGATTTTTATTCATTTATTGATGCTTTTGTCCCAATGCGGTGTTTGTTTTTGTAGATTGTCACTCTTGGAATGCCTGTTATATCACCTGTTGAAAGGGTTGGACAATATGTGAAACCAAGGGATTGGAATGCACTGATAAGTGATCCAGATACTGTGAGTAGTTTTACTTGTCTTAGTTTTTGTTAAATTAGGGTTTATTGGGTTTATTCTTATCTAATATTTTCCATATTTGTATGTTTCCTTTATTAGTATTATGGTCTCTATTTATTCACCAATGTAATAGGTTATTATGTATACTTTTATTTATTCAATATAGTATATAAAATTTAACCGTTTTAATTATATTATTTAAGTTACCAATTGTTTACGTTGGATTCAGTTTTCTGCATATGTTTTGATTATTGAACTGTTATTGAACTTTAAAATGTAACATCACGATGGGAACTGCATTCCGTTGGTTAAATGAGCATATATATTCTGATTGTCTGGCCTAGCATTCAGAGATTATATACTTCTTCTCATTGAAGAGAATAAGGCGACCTTGCTAAATAAGTAAATGATAATGTAGGTGGTTATTGATGTGCGAAATCACTACGAAACAAGGATTGGGAAGTTCAAAACAGCAGTTGACCCTTGTACTACAGCATTTCGAGATTTTCCAGCTTGGGTGGAAGATAAACTCCAACTCCCTAAATTAGATTGCAAGCATTCAAATCAAGGAGGTGAAGAAACAGTGAACCCTAAAGAGAGCTCGCCTCCACGAGTTGCTATGTATTGCACTGGAGGGATCCGTTGTGAGAAAGCCTCGAGTTATCTCCTCAGCAAAGGCTTTAAAGAGGTGAACTTGTTGATCTCTATATAATATGATTTGCTTTTGATCAACTTTTTGGATAAACTTTGGACATGACATATGTTCGTCAAAGCTCAAACGCAATAGGACTTGTAGGGAAAATCCTAATAATTATTTTCTTTTCAAAATAATAATAATCTTGATGAAGGGCTGCGTAGAAGGTGCACGCCTTGCCTTTTTAAGTTCTTTTGGATAATTTCGTCCTTGAAAAATCACAAGCTTTTCAGAAATAAATGTTCCTAGATTGATGATCTATGCATGCACTTAAATCTGGAAAATGTTTAATCAAATTATTTTTGCATGACACGAAACTGCATTCTCATGTATTTTTCTGGCCGAGACAGTAATAACCTGATGCATCATCTTATCTCAATCTTTTTTAGGTTTATCATTTGGAAGGTGGAATTCTGAAATATCTGGAGGAAGTTCCAAAGTCAGAGAGCCTATGGGAAGGTGAATGCTTTGTATTTGACAAGCGTGTCTCGGTTGAGCATGGTCTGGAGCAGGGCACATTCAAGCTCTGCTATGGGTGCAAGCAACCAGTGAGTGATGCTGACATGGAATCTCCTGAGTGGGAGTATGGGGTTTCTTGTCCTTACTGTTTCTCAACGAAATCTGAAGAAGAGAAGGAGAGAGCAAGAGCTCGGCAAAGGCAATTTGAGACTTGGGGCATCATAGGTGGTCCGGACAAAGGCCGGCGTCCAACACAAAATGATGACAATGTTAGTCATCCAAATTCAGTTTGATGCCTTGCTGCTTAAGGTTCTTGTCATTTTTGTATTAGGGAATTATACAAAGGACAGATCAAAATATCCATCTCCACTTATTAATTATTGTCTTGTGTACAATATATCATTTTTTTGTTGATTATGACAAAATTGAGAAATTTTGGAACTTGGTCCTCTGTTAAATGAACTTCGATACTTTCAGATTCGATGACTTTCTCCCAGCTCTTTTCTGTCTCTCTGTTTTAACGACACGTGAGCAACAAAGAAAGCCGTAAAGTGATGAGAACAAAACAAAGATTTATTAAACTAAATTCGGTACAAGTATGGCTCCTTTAGGGCTAAAGAGAGAAAGAAACATATAACAAATGAAGACAGGTGAAAAGGGTTTGTTGAAGTTCCTCCATCCAAAGCTCAGGAATGTCCTACATCCCATCAACATTGAAGCTGCTGCGTTATGGAGTGTTGCTGGGATAGCCACTGCTCTTTGGCTTGTACAGGTATTGGTCTTTCTTTTCTTTTCATCTACCATCATGTTTCTGTTTGTTCAATGCTATTTGTGAAAGATCGTAACTATTCTTCTCGTCTTGTAACTGAAAAACATGGCAGCCATTTGACTGGTTGAAGAAGAAGGTTTGGGCGGTAGAGGAGAAAGGAGAAAGCGGGGTGCAAACTAATGAATAAGATAGCTAGTGTAAAAATATATCCACTATCTGAAGAACTCTAATATCTGAATAAAGATTTGATATGATATGAACATGTCCATCCTTCTTCTTCTTTCTATTTCTTCAAATTCATCATCAGGATTCAAGAATGGTGAGGAGGAGAAGGAGGAGGAGCAACAGCGTCAGAATGTTGCTTGAGAGCTTGGAAAACTTCAGAACTGGTACTGAAAATCTTAGCTCTTCTATTATAATCTGTTGGGTCTCTCCCTTCATCTTCTCCCATAATGGCTTCTTTTTCCAAACATTCAAGTAGCTCCACAATTCCCTTTCCTCCGCCGCCGCACTTCTCCTCCAATCTCCCTAGCTTCAGCCACTCTTCTATGTCCACCTCCCCCACACCCACAGAGCTTCCCCATCCACCCTGACTAACACATCTTGGCTCTGCCCCTGCCTCCGCAGGTCGATGGCAGAGCCATGGTGAGGAAAGGGATGGAGTTGGCCTGAGGTTGGGGAAGAAGGCGGTGGAGGGGCGGTGGAGGAGGGGAGGAGGTGGTGGAGACATGAGAGATGCTGAGAGTTGCATATTGTGTTGGTGTGGTGGTGAGGTGTGTTTATGAATGATTGATAAAATGATGGGCTTCCTTTCTTCTTGTTTGAGAGATTTGGGGAGGGGTGTTTGGAGTATGTATTATTATAGGAATTT

Coding sequence (CDS)

AACGGAGAAAGAACGAAGAACGCGAACGACTCGTCGTCCTCACCCTCCATGCTCATCAGATATAGAGCTCCTCCATTGTTTCCTCTGGGAATGGTATCGCCATTTCCTTCTTCTTCCTCCTCCTCCTTTTCTTCCCTTTCTTCCTTTTCTTCAAACTCTAATCTCATCTCATTCAATTTCAGTCCCTCTTTCCCTATTCCAAGACCCTCCCTTTCTTTCCCTTCGCTGCTCTCCGCTTTCCTTCACAAGCCCTGTCGGAGCATGACTCATTCCGTTTGCTTCTCTGGACCTTCCCCCACCGCTCCCATTCATAGCTCATCTGAAGAATCTGAGGATGTGGAATCAGCTTCTCTTGTTGTTGTTTCTTTCTATAAGTTCGCTGATTTTCCTGACCATGCCGAATTTCGCTTGCCCTTGAAGAAGCTCTGCGAGGAACTGCGTGTTTCAGGAGGTATCATTCTCGCACCTGAAGGAATTAATGGGAGCATATGTGGATCTCGGGAGTCTGTCCAAAGGGTTCTTGGATTCATCCAAAGTGATGAACGGCTAAAAGGACTAAGGCAGGTGGAAACACCTGTTAGTCCTGAAGAAGAAGCCATCCACAACGGTCATACTAGCAGTTCCCCTCTTGCAGCGGGGGAAGATGCACCCTTTCGTTGGGACCATGTCAGGGTCAAATTAAAGAAAGAGATTGTCACTCTTGGAATGCCTGTTATATCACCTGTTGAAAGGGTTGGACAATATGTGAAACCAAGGGATTGGAATGCACTGATAAGTGATCCAGATACTGTGGTTATTGATGTGCGAAATCACTACGAAACAAGGATTGGGAAGTTCAAAACAGCAGTTGACCCTTGTACTACAGCATTTCGAGATTTTCCAGCTTGGGTGGAAGATAAACTCCAACTCCCTAAATTAGATTGCAAGCATTCAAATCAAGGAGGTGAAGAAACAGTGAACCCTAAAGAGAGCTCGCCTCCACGAGTTGCTATGTATTGCACTGGAGGGATCCGTTGTGAGAAAGCCTCGAGTTATCTCCTCAGCAAAGGCTTTAAAGAGGTTTATCATTTGGAAGGTGGAATTCTGAAATATCTGGAGGAAGTTCCAAAGTCAGAGAGCCTATGGGAAGGTGAATGCTTTGTATTTGACAAGCGTGTCTCGGTTGAGCATGGTCTGGAGCAGGGCACATTCAAGCTCTGCTATGGGTGCAAGCAACCAGTGAGTGATGCTGACATGGAATCTCCTGAGTGGGAGTATGGGGTTTCTTGTCCTTACTGTTTCTCAACGAAATCTGAAGAAGAGAAGGAGAGAGCAAGAGCTCGGCAAAGGCAATTTGAGACTTGGGGCATCATAGGTGGTCCGGACAAAGGCCGGCGTCCAACACAAAATGATGACAATGTTAGTCATCCAAATTCAGTTTGA

Protein sequence

NGERTKNANDSSSSPSMLIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPSLSFPSLLSAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFRLPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPEEEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNALISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGEETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKERARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV
Homology
BLAST of Cucsat.G17487 vs. ExPASy Swiss-Prot
Match: Q1JPN0 (Rhodanese-like domain-containing protein 7 OS=Arabidopsis thaliana OX=3702 GN=STR7 PE=2 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 4.3e-163
Identity = 304/466 (65.24%), Postives = 345/466 (74.03%), Query Frame = 0

Query: 21  YRAPPLFPL--GMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPSLSFPSLLS 80
           +R PP   +   + SP P  S S F   S    NSN +  N  P    P+   S P  LS
Sbjct: 7   WRFPPSLAVVRMLSSPPPPHSHSPF---SGGGVNSNSVGGNSKPELQFPQ---SQPHKLS 66

Query: 81  AFLHKPCRSMTHSVCFSGP-------SPTAPIHSSSEESEDVESASLVVVSFYKFADFPD 140
           +    P  S+  +V  S         +  +   S   ES D +  SLVVVSFYKFADFP+
Sbjct: 67  S---SPSSSLKSTVACSNAGAIRRSMATVSQAFSERTESIDSDLGSLVVVSFYKFADFPE 126

Query: 141 HAEFRLPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVET 200
           HA+FR PLK LCE+LRVSGGIILAPEGINGSICG RESV+ VL FIQ D RL GLRQVET
Sbjct: 127 HADFRKPLKDLCEKLRVSGGIILAPEGINGSICGIRESVEEVLAFIQRDVRLNGLRQVET 186

Query: 201 PVSPEEEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKP 260
           PVSPE+EAIH+GH+SSSPLAAGEDAPFRWDHVRVKLKKEIVTLG+P +SP+ERVG YV P
Sbjct: 187 PVSPEQEAIHHGHSSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGIPSVSPIERVGTYVSP 246

Query: 261 RDWNALISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQL-------- 320
            +WN LISDP+TVVIDVRN YETRIGKFK AVDPCTTAFR+FP+WVE++  L        
Sbjct: 247 EEWNELISDPETVVIDVRNTYETRIGKFKGAVDPCTTAFRNFPSWVENQFALKQEGNETQ 306

Query: 321 ---PKLDCKHSNQGGEETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGG 380
               K D        ++   PK  + PR+AMYCTGGIRCEKASS LLS+GF+EVYHL+GG
Sbjct: 307 AKVEKEDFSEITHKEDKAEKPK--TLPRIAMYCTGGIRCEKASSLLLSQGFEEVYHLKGG 366

Query: 381 ILKYLEEVPKSESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYG 440
           ILKYLEEVPK+ESLWEGECFVFDKRVSVEHGL QGT KLCYGCKQP+SD DME+PE+EYG
Sbjct: 367 ILKYLEEVPKTESLWEGECFVFDKRVSVEHGLAQGTHKLCYGCKQPISDEDMEAPEYEYG 426

Query: 441 VSCPYCFSTKSEEEKERARARQRQFETWGIIGGPDKGRRPTQNDDN 467
           VSCPYC+S KSEEEKERARARQ QFE WG+IGGPDKGRRP    D+
Sbjct: 427 VSCPYCYSKKSEEEKERARARQTQFEEWGVIGGPDKGRRPATKPDS 461

BLAST of Cucsat.G17487 vs. ExPASy Swiss-Prot
Match: Q1GYV1 (tRNA uridine(34) hydroxylase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) OX=265072 GN=trhO PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 4.0e-84
Identity = 155/329 (47.11%), Postives = 205/329 (62.31%), Query Frame = 0

Query: 118 LVVVSFYKFADFPDHAEFRLPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFI 177
           +VV + YKF   PD+ E +  L  LC    + G ++LA EGING++ GSR ++  +L ++
Sbjct: 4   IVVAALYKFVSLPDYQELQPGLLALCNAQGIKGTLLLAEEGINGTVAGSRAAIDALLTYL 63

Query: 178 QSDERLKGLRQVETPVSPEEEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMP 237
           + D R+  +   E                    +  ++ PF    ++V+LKKEIVTLG+P
Sbjct: 64  RQDSRMVDIDHKE--------------------SFADEMPFY--RMKVRLKKEIVTLGVP 123

Query: 238 VISPVERVGQYVKPRDWNALISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWV 297
            ISP ++VG YVKP DWN LISDPD VVID RN+YE  IG FK A+DP TT FR FP +V
Sbjct: 124 GISPNKKVGTYVKPEDWNTLISDPDVVVIDTRNNYEYEIGTFKGAIDPATTTFRQFPEFV 183

Query: 298 EDKLQLPKLDCKHSNQGGEETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHL 357
              L   K                      +VAM+CTGGIRCEKASSY+L +GFKEVYHL
Sbjct: 184 RKNLDPAK--------------------NKKVAMFCTGGIRCEKASSYMLEQGFKEVYHL 243

Query: 358 EGGILKYLEEVPKSESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEW 417
           +GGILKYLE +P+ ESLWEGECFVFD+RV+V+HGL+ G +  C+ C+ P+S ADM S ++
Sbjct: 244 QGGILKYLETIPEEESLWEGECFVFDQRVAVKHGLQVGDYDQCFACRHPISAADMASSKY 290

Query: 418 EYGVSCPYCFSTKSEEEKERARARQRQFE 447
             G+SCP C+   S E++ R   RQRQ E
Sbjct: 304 VKGISCPRCYDRMSAEKRARIAERQRQIE 290

BLAST of Cucsat.G17487 vs. ExPASy Swiss-Prot
Match: Q92LX6 (tRNA uridine(34) hydroxylase OS=Rhizobium meliloti (strain 1021) OX=266834 GN=trhO PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 5.7e-83
Identity = 155/326 (47.55%), Postives = 209/326 (64.11%), Query Frame = 0

Query: 119 VVVSFYKFADFPDHAEFRLPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQ 178
           +V + Y F  FP  A+FR PL+ +C+   V G ++LA EGING+I G+ E +  VL  ++
Sbjct: 18  LVAALYHFVSFPRFADFREPLQAICDANGVKGTLLLAHEGINGTIAGTEEGIAAVLACVR 77

Query: 179 SDERLKGLRQVETPVSPEEEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPV 238
           +     GL             IH    +S+        PF    ++V+LKKEIVT+G+  
Sbjct: 78  AQPEFAGL-------------IHKESRASA-------MPFL--RMKVRLKKEIVTMGVEN 137

Query: 239 ISPVERVGQYVKPRDWNALISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVE 298
           I P + VG YV+P+DWNALISDP+T+VID RN YET IG F+ AVDP T  FR+FP WV 
Sbjct: 138 IDPKKVVGTYVEPKDWNALISDPETLVIDTRNDYETAIGLFQGAVDPKTKTFREFPEWVR 197

Query: 299 DKLQLPKLDCKHSNQGGEETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLE 358
           +                    N    + P++AMYCTGGIRCEKA++++  +GF+EVYHL+
Sbjct: 198 N--------------------NSGLHNKPKIAMYCTGGIRCEKATAFMKEQGFEEVYHLK 257

Query: 359 GGILKYLEEVPKSESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWE 418
           GGILKYLEE+P  ESLWEG CFVFD+RVSV HGL++G   LC+ C+QP++  D++SP  E
Sbjct: 258 GGILKYLEEIPPEESLWEGACFVFDERVSVTHGLQEGEHTLCHACRQPLTPEDLQSPLHE 301

Query: 419 YGVSCPYCFSTKSEEEKERARARQRQ 445
            GVSC +C  T++EE++ER R RQRQ
Sbjct: 318 EGVSCIHCHDTRTEEDRERYRQRQRQ 301

BLAST of Cucsat.G17487 vs. ExPASy Swiss-Prot
Match: B2IVX7 (tRNA uridine(34) hydroxylase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=trhO PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 9.8e-83
Identity = 153/329 (46.50%), Postives = 211/329 (64.13%), Query Frame = 0

Query: 119 VVVSFYKFADFPDHAEFRLPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQ 178
           +V + YKF   PD A+ R PL   C+   V G I+LA EGING+I GSR+++  VL F++
Sbjct: 8   IVAALYKFVKLPDFAQKRDPLLSYCQAQGVKGTILLAQEGINGTIAGSRQAIDSVLWFLR 67

Query: 179 SDERLKGLRQVETPVSPEEEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPV 238
           SD RL  L   E+            +T + P          ++ ++V+LK EIVTLG+P 
Sbjct: 68  SDPRLADLEYKES------------YTETPP----------FERMKVRLKSEIVTLGLPE 127

Query: 239 ISPVERVGQYVKPRDWNALISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVE 298
           I P E+VG YV P++WN LI DP+  VID RN YE  IG F+ A +P T +FR+FP +V 
Sbjct: 128 IDPTEKVGTYVSPQEWNDLICDPEVTVIDTRNDYEVNIGTFQGAENPQTGSFREFPDYVV 187

Query: 299 DKLQLPKLDCKHSNQGGEETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLE 358
             L       KH                 +VA++CTGGIRCEKASS++L++GF EVYHL+
Sbjct: 188 HNLD----PTKHK----------------KVALFCTGGIRCEKASSFMLAQGFAEVYHLK 247

Query: 359 GGILKYLEEVPKSESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWE 418
           GGILKYLEEVP  ESLW+GECFVFD+RV+V HGLE+G+++LC+ C  P+S+ D  SP++E
Sbjct: 248 GGILKYLEEVPAQESLWQGECFVFDERVAVSHGLEEGSYELCFCCGYPISEEDKISPKYE 294

Query: 419 YGVSCPYCFSTKSEEEKERARARQRQFET 448
            G+SC YCF + +EE++ R + + R ++T
Sbjct: 308 QGISCAYCFDSLTEEKRARQQEKWRHYQT 294

BLAST of Cucsat.G17487 vs. ExPASy Swiss-Prot
Match: Q8YZS3 (tRNA uridine(34) hydroxylase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=trhO PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 9.8e-83
Identity = 153/328 (46.65%), Postives = 212/328 (64.63%), Query Frame = 0

Query: 119 VVVSFYKFADFPDHAEFRLPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQ 178
           +V +FYKF   PD AE + PL   C    + G I+LA EGING+I GSR S+  VL +++
Sbjct: 8   IVAAFYKFVSLPDFAEKQEPLLAYCLAQNIKGTILLAKEGINGTIAGSRLSIDNVLSYLR 67

Query: 179 SDERLKGLRQVETPVSPEEEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPV 238
           SD RL+ L              H   T+ +P       PF  + ++V+LKKEIVTLG+P 
Sbjct: 68  SDLRLQDLE-------------HKESTADTP-------PF--ERMKVRLKKEIVTLGLPE 127

Query: 239 ISPVERVGQYVKPRDWNALISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVE 298
           + P E+VG YV P +WN LISDP+ +VID RN YE  IG F+ A +P T +FRDFP +V 
Sbjct: 128 VDPNEQVGAYVTPEEWNELISDPEVIVIDTRNDYEVHIGTFQGAQNPQTNSFRDFPEYVR 187

Query: 299 DKLQLPKLDCKHSNQGGEETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLE 358
             L                  +P +    +VAM+CTGGIRCEKASS++LS+GF EVYHL+
Sbjct: 188 QNL------------------DPNQHK--KVAMFCTGGIRCEKASSFMLSQGFAEVYHLK 247

Query: 359 GGILKYLEEVPKSESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWE 418
           GGILKYLE++P  ESLW+GECFVFD+R++V HGLE GT +LC+ C  P+++ D  S ++E
Sbjct: 248 GGILKYLEQIPPEESLWQGECFVFDERIAVVHGLEPGTHELCFCCGHPLAEEDKASLQYE 293

Query: 419 YGVSCPYCFSTKSEEEKERARARQRQFE 447
            G+SCP+CF + +E+++ R + + RQ++
Sbjct: 308 EGISCPHCFDSLTEDKRMRQQEKWRQYQ 293

BLAST of Cucsat.G17487 vs. NCBI nr
Match: XP_004146431.2 (rhodanese-like domain-containing protein 7 [Cucumis sativus])

HSP 1 Score: 928 bits (2398), Expect = 0.0
Identity = 456/457 (99.78%), Postives = 456/457 (99.78%), Query Frame = 0

Query: 17  MLIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPSLSFPSL 76
           MLIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRP LSFPSL
Sbjct: 1   MLIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPFLSFPSL 60

Query: 77  LSAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFR 136
           LSAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFR
Sbjct: 61  LSAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFR 120

Query: 137 LPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPE 196
           LPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPE
Sbjct: 121 LPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPE 180

Query: 197 EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNA 256
           EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNA
Sbjct: 181 EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNA 240

Query: 257 LISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGE 316
           LISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGE
Sbjct: 241 LISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGE 300

Query: 317 ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE 376
           ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE
Sbjct: 301 ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE 360

Query: 377 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE 436
           GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE
Sbjct: 361 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE 420

Query: 437 RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV 473
           RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV
Sbjct: 421 RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV 457

BLAST of Cucsat.G17487 vs. NCBI nr
Match: XP_016902023.1 (PREDICTED: LOW QUALITY PROTEIN: rhodanese-like domain-containing protein 7 [Cucumis melo])

HSP 1 Score: 825 bits (2132), Expect = 4.58e-300
Identity = 410/457 (89.72%), Postives = 427/457 (93.44%), Query Frame = 0

Query: 17  MLIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPSLSFPSL 76
           MLIRYRAPPL  LGMVSP PSSSSSSFSS      N+NLISFNFSPSFP+P+PS SFPSL
Sbjct: 1   MLIRYRAPPLLALGMVSPLPSSSSSSFSS------NTNLISFNFSPSFPVPKPSSSFPSL 60

Query: 77  LSAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFR 136
           LS+F+HKP RSMTHS+CFSGPSPTAPIHSS EESED++S SLVVVSFYKFA FPDHAEFR
Sbjct: 61  LSSFVHKPSRSMTHSICFSGPSPTAPIHSSCEESEDMDSISLVVVSFYKFAHFPDHAEFR 120

Query: 137 LPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPE 196
            PLKKLCEELRV GGIILAPEGINGSICGSRESVQRVLGFI+SDERLKGLRQVET VSPE
Sbjct: 121 SPLKKLCEELRVLGGIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETSVSPE 180

Query: 197 EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNA 256
           EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRD N+
Sbjct: 181 EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDXNS 240

Query: 257 LISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGE 316
           LISDPDTVV DV+NHYETRI KFKTAVDP TTAFRDFPAWVEDK QLPKLDCKHSNQG E
Sbjct: 241 LISDPDTVVTDVQNHYETRIEKFKTAVDPSTTAFRDFPAWVEDKFQLPKLDCKHSNQGDE 300

Query: 317 ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE 376
           ETVNP+ESSP RVAMYCTGGIRCEKASSYLL KGFKEVYHLEGGILKYLEEVP+SESL E
Sbjct: 301 ETVNPEESSPLRVAMYCTGGIRCEKASSYLLRKGFKEVYHLEGGILKYLEEVPRSESLRE 360

Query: 377 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE 436
           GECFVFDKR SVEHGLEQGTFKLCYG KQPVSDADMESP+WEYGVSCPYCFS KS+EEKE
Sbjct: 361 GECFVFDKRDSVEHGLEQGTFKLCYGYKQPVSDADMESPQWEYGVSCPYCFSMKSDEEKE 420

Query: 437 RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV 473
           RARARQRQFETWGIIGGPDKGRRPTQND+NV+H NSV
Sbjct: 421 RARARQRQFETWGIIGGPDKGRRPTQNDNNVNHLNSV 451

BLAST of Cucsat.G17487 vs. NCBI nr
Match: KAE8648056.1 (hypothetical protein Csa_005784 [Cucumis sativus])

HSP 1 Score: 800 bits (2067), Expect = 3.12e-291
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 0

Query: 88  MTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFRLPLKKLCEELR 147
           MTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFRLPLKKLCEELR
Sbjct: 1   MTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFRLPLKKLCEELR 60

Query: 148 VSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPEEEAIHNGHTSS 207
           VSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPEEEAIHNGHTSS
Sbjct: 61  VSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPEEEAIHNGHTSS 120

Query: 208 SPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNALISDPDTVVID 267
           SPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNALISDPDTVVID
Sbjct: 121 SPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNALISDPDTVVID 180

Query: 268 VRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGEETVNPKESSPP 327
           VRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGEETVNPKESSPP
Sbjct: 181 VRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGEETVNPKESSPP 240

Query: 328 RVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWEGECFVFDKRVS 387
           RVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWEGECFVFDKRVS
Sbjct: 241 RVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWEGECFVFDKRVS 300

Query: 388 VEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKERARARQRQFET 447
           VEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKERARARQRQFET
Sbjct: 301 VEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKERARARQRQFET 360

Query: 448 WGIIGGPDKGRRPTQNDDNVSHPNSV 473
           WGIIGGPDKGRRPTQNDDNVSHPNSV
Sbjct: 361 WGIIGGPDKGRRPTQNDDNVSHPNSV 386

BLAST of Cucsat.G17487 vs. NCBI nr
Match: XP_038892558.1 (rhodanese-like domain-containing protein 7 [Benincasa hispida])

HSP 1 Score: 786 bits (2030), Expect = 1.17e-284
Identity = 394/456 (86.40%), Postives = 410/456 (89.91%), Query Frame = 0

Query: 18  LIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPSLSFPSLL 77
           ++RYRA PL  LGMVSP PSSS SSFSS      N N I FN  PSFPIP+PS S P  L
Sbjct: 1   MLRYRASPLLALGMVSPLPSSSPSSFSS------NPNFIPFN--PSFPIPKPSSSLPLFL 60

Query: 78  SAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFRL 137
           S+FL KP RSMTHS CFSGPSPT        E E+++SASLVVVSFYKFAD PDHAEFR 
Sbjct: 61  SSFLQKPTRSMTHSGCFSGPSPTPC------EVEEMDSASLVVVSFYKFADLPDHAEFRS 120

Query: 138 PLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPEE 197
           PLK LCEELRVSGGIILAPEGINGSICG+RESVQ+VLGFI+SDERLKGLRQVETPVSPEE
Sbjct: 121 PLKNLCEELRVSGGIILAPEGINGSICGTRESVQKVLGFIESDERLKGLRQVETPVSPEE 180

Query: 198 EAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNAL 257
           EAIH+GHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNAL
Sbjct: 181 EAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNAL 240

Query: 258 ISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQG-GE 317
           ISDPD VVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDK QLPK DC+HSNQG  E
Sbjct: 241 ISDPDIVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKFQLPKSDCEHSNQGVEE 300

Query: 318 ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE 377
           ETVNP+E SPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLW 
Sbjct: 301 ETVNPEEDSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWV 360

Query: 378 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE 437
           GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYC+ TKSEEEKE
Sbjct: 361 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCYFTKSEEEKE 420

Query: 438 RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNS 472
           RARARQRQFETWGIIGGPDKGRRPTQ+D+N   PNS
Sbjct: 421 RARARQRQFETWGIIGGPDKGRRPTQSDNNDDPPNS 442

BLAST of Cucsat.G17487 vs. NCBI nr
Match: KAG7031505.1 (Rhodanese-like domain-containing protein 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 781 bits (2017), Expect = 1.51e-282
Identity = 388/457 (84.90%), Postives = 418/457 (91.47%), Query Frame = 0

Query: 18  LIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPSLSFPSLL 77
           ++RYRAPPL  LGMVSP  SSSSSS SS   FSSN NLI+FN  PSFP+ +PS + PSLL
Sbjct: 1   MLRYRAPPLLALGMVSPLLSSSSSSSSS---FSSNPNLITFN--PSFPVLKPSPTLPSLL 60

Query: 78  SAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFRL 137
           S+F  KP RSMTHS CFSGPSP  PI ++ E +++V+S SLVVVSFYKFADFPDHAEFR 
Sbjct: 61  SSFHQKPTRSMTHSKCFSGPSPAVPIPTACE-AQEVDSVSLVVVSFYKFADFPDHAEFRS 120

Query: 138 PLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPEE 197
           PLKKLC+ELRVSGGIILAPEGINGSICGSRESVQRVLGFI+SDERLKGLRQVETPVSPEE
Sbjct: 121 PLKKLCQELRVSGGIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEE 180

Query: 198 EAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNAL 257
           EAIH+GH+S SPLAAGEDAPFRWDHVRVKLKKEIVTLGMP +SPVERVGQYVKPRDWNAL
Sbjct: 181 EAIHHGHSSGSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPDVSPVERVGQYVKPRDWNAL 240

Query: 258 ISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGE- 317
           ISDPDTVVIDVRNHYETRIGKFK AVDPCT AFRDFP WVEDK QLPK DC+HSN+  E 
Sbjct: 241 ISDPDTVVIDVRNHYETRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEG 300

Query: 318 ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE 377
           +TVNP+E+SPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPK+ESLWE
Sbjct: 301 KTVNPEENSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWE 360

Query: 378 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE 437
           GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESP+WEYGVSCP+C+STKSEEEKE
Sbjct: 361 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKE 420

Query: 438 RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV 473
           RARARQRQFETWGIIGGPDKGRRP Q+D N + PNS+
Sbjct: 421 RARARQRQFETWGIIGGPDKGRRPKQSDKNATLPNSI 451

BLAST of Cucsat.G17487 vs. ExPASy TrEMBL
Match: A0A0A0KM63 (Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G223080 PE=3 SV=1)

HSP 1 Score: 928 bits (2398), Expect = 0.0
Identity = 456/457 (99.78%), Postives = 456/457 (99.78%), Query Frame = 0

Query: 17  MLIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPSLSFPSL 76
           MLIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRP LSFPSL
Sbjct: 1   MLIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPFLSFPSL 60

Query: 77  LSAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFR 136
           LSAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFR
Sbjct: 61  LSAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFR 120

Query: 137 LPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPE 196
           LPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPE
Sbjct: 121 LPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPE 180

Query: 197 EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNA 256
           EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNA
Sbjct: 181 EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNA 240

Query: 257 LISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGE 316
           LISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGE
Sbjct: 241 LISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGE 300

Query: 317 ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE 376
           ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE
Sbjct: 301 ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE 360

Query: 377 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE 436
           GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE
Sbjct: 361 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE 420

Query: 437 RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV 473
           RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV
Sbjct: 421 RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV 457

BLAST of Cucsat.G17487 vs. ExPASy TrEMBL
Match: A0A1S4E1C5 (LOW QUALITY PROTEIN: rhodanese-like domain-containing protein 7 OS=Cucumis melo OX=3656 GN=LOC103496761 PE=3 SV=1)

HSP 1 Score: 825 bits (2132), Expect = 2.22e-300
Identity = 410/457 (89.72%), Postives = 427/457 (93.44%), Query Frame = 0

Query: 17  MLIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPSLSFPSL 76
           MLIRYRAPPL  LGMVSP PSSSSSSFSS      N+NLISFNFSPSFP+P+PS SFPSL
Sbjct: 1   MLIRYRAPPLLALGMVSPLPSSSSSSFSS------NTNLISFNFSPSFPVPKPSSSFPSL 60

Query: 77  LSAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFR 136
           LS+F+HKP RSMTHS+CFSGPSPTAPIHSS EESED++S SLVVVSFYKFA FPDHAEFR
Sbjct: 61  LSSFVHKPSRSMTHSICFSGPSPTAPIHSSCEESEDMDSISLVVVSFYKFAHFPDHAEFR 120

Query: 137 LPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPE 196
            PLKKLCEELRV GGIILAPEGINGSICGSRESVQRVLGFI+SDERLKGLRQVET VSPE
Sbjct: 121 SPLKKLCEELRVLGGIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETSVSPE 180

Query: 197 EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNA 256
           EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRD N+
Sbjct: 181 EEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDXNS 240

Query: 257 LISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGE 316
           LISDPDTVV DV+NHYETRI KFKTAVDP TTAFRDFPAWVEDK QLPKLDCKHSNQG E
Sbjct: 241 LISDPDTVVTDVQNHYETRIEKFKTAVDPSTTAFRDFPAWVEDKFQLPKLDCKHSNQGDE 300

Query: 317 ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE 376
           ETVNP+ESSP RVAMYCTGGIRCEKASSYLL KGFKEVYHLEGGILKYLEEVP+SESL E
Sbjct: 301 ETVNPEESSPLRVAMYCTGGIRCEKASSYLLRKGFKEVYHLEGGILKYLEEVPRSESLRE 360

Query: 377 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE 436
           GECFVFDKR SVEHGLEQGTFKLCYG KQPVSDADMESP+WEYGVSCPYCFS KS+EEKE
Sbjct: 361 GECFVFDKRDSVEHGLEQGTFKLCYGYKQPVSDADMESPQWEYGVSCPYCFSMKSDEEKE 420

Query: 437 RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV 473
           RARARQRQFETWGIIGGPDKGRRPTQND+NV+H NSV
Sbjct: 421 RARARQRQFETWGIIGGPDKGRRPTQNDNNVNHLNSV 451

BLAST of Cucsat.G17487 vs. ExPASy TrEMBL
Match: A0A6J1FRM3 (rhodanese-like domain-containing protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111447816 PE=3 SV=1)

HSP 1 Score: 773 bits (1997), Expect = 8.11e-280
Identity = 384/457 (84.03%), Postives = 416/457 (91.03%), Query Frame = 0

Query: 18  LIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPSLSFPSLL 77
           ++RYRAPPL  LGMVSP  +SSSSS SS   FSSN NLI+FN  PSF + +PS + PSLL
Sbjct: 1   MLRYRAPPLLALGMVSPLLTSSSSSSSS---FSSNPNLITFN--PSFLVLKPSPTLPSLL 60

Query: 78  SAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFRL 137
           S+F  KP RSMTHS CFSGPSP  PI ++ E +++V+S SLVVVSFYKFADFPDHAEFR 
Sbjct: 61  SSFHQKPTRSMTHSKCFSGPSPAVPIPTACE-AQEVDSVSLVVVSFYKFADFPDHAEFRS 120

Query: 138 PLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPEE 197
           PLKKLC+ELRVSGGIILAPEGINGSICGSRESVQRVLGFI+SDERLKGLRQVETPVSPEE
Sbjct: 121 PLKKLCQELRVSGGIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEE 180

Query: 198 EAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNAL 257
           EAIH+GH+S SPLAAGEDAPFRWDHVRVKLKKEIVTLGMP +SPVER GQYVKPRDWNAL
Sbjct: 181 EAIHHGHSSGSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPDVSPVERAGQYVKPRDWNAL 240

Query: 258 ISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGE- 317
           ISDPDTVVIDVRNHYETRIGKFK AVDPCT AFRDFP WVEDK QLPK DC+HSN+  E 
Sbjct: 241 ISDPDTVVIDVRNHYETRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEG 300

Query: 318 ETVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWE 377
           +TVNP+++SPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPK+ESLWE
Sbjct: 301 KTVNPEQNSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWE 360

Query: 378 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKE 437
           GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESP+WEYGVSCP+C+STKSEEEKE
Sbjct: 361 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKE 420

Query: 438 RARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV 473
           RARARQRQFETWGIIGGPDKGRRP Q+D N + PNS+
Sbjct: 421 RARARQRQFETWGIIGGPDKGRRPKQSDKNATLPNSI 451

BLAST of Cucsat.G17487 vs. ExPASy TrEMBL
Match: A0A6J1JDN9 (rhodanese-like domain-containing protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111485926 PE=3 SV=1)

HSP 1 Score: 759 bits (1960), Expect = 2.07e-274
Identity = 380/444 (85.59%), Postives = 404/444 (90.99%), Query Frame = 0

Query: 31  MVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPSLSFPSLLSAFLHKPCRSMTH 90
           MVSP  SSSSS     SSFSSN NLI+FN  PSFPI +PS + PSLLS+F  KP RSMTH
Sbjct: 1   MVSPLLSSSSS----YSSFSSNPNLITFN--PSFPILKPSPTLPSLLSSFHQKPSRSMTH 60

Query: 91  SVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFRLPLKKLCEELRVSG 150
           S CFSGPSP  PI S+ E  E V+S SLVVVSFYKFADFPDHAEFR PLKKLC+ELRVSG
Sbjct: 61  SKCFSGPSPAVPIPSACEVQE-VDSMSLVVVSFYKFADFPDHAEFRSPLKKLCQELRVSG 120

Query: 151 GIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPEEEAIHNGHTSSSPL 210
           GIILAPEGINGSICGSRESVQRVLGFI+SDERLKGLRQVETPVSPEEEAIH+GH+SSSPL
Sbjct: 121 GIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHSSSSPL 180

Query: 211 AAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNALISDPDTVVIDVRN 270
           AAGEDAPFRWDHVRVKLKKEIVTLGMP +SPVERVGQYVKPRDWN LISDPDTVVIDVRN
Sbjct: 181 AAGEDAPFRWDHVRVKLKKEIVTLGMPDVSPVERVGQYVKPRDWNTLISDPDTVVIDVRN 240

Query: 271 HYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQGGE-ETVNPKESSPPRV 330
           HYETRIGKFK AVDPCT AFRDFP WVEDK QLPK DC+HSN+  E +TVNP+E+SPPRV
Sbjct: 241 HYETRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNPEENSPPRV 300

Query: 331 AMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWEGECFVFDKRVSVE 390
           AMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPK+ESLWEGECFVFDKRVSVE
Sbjct: 301 AMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVE 360

Query: 391 HGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKERARARQRQFETWG 450
           HGLEQGTFKLCYGCKQPVSDAD+ESP+WEYGVSCP+C+STKSEEEKERARARQRQFETWG
Sbjct: 361 HGLEQGTFKLCYGCKQPVSDADIESPQWEYGVSCPHCYSTKSEEEKERARARQRQFETWG 420

Query: 451 IIGGPDKGRRPTQNDDNVSHPNSV 473
           IIGGPDKGRRP Q+D N + PNS+
Sbjct: 421 IIGGPDKGRRPKQSDKNATLPNSI 437

BLAST of Cucsat.G17487 vs. ExPASy TrEMBL
Match: A0A6J1CYE5 (rhodanese-like domain-containing protein 7 OS=Momordica charantia OX=3673 GN=LOC111015925 PE=3 SV=1)

HSP 1 Score: 754 bits (1946), Expect = 3.15e-271
Identity = 387/476 (81.30%), Postives = 419/476 (88.03%), Query Frame = 0

Query: 6   KNANDSSSSPSMLIRYRAPPLFPLGMVSPFP----SSSSSSFSSLSSFSSNSNLISFNFS 65
           K  NDSSS+ + +IRYRA     L MVSP P    SSSSSS SS SSFSSN N I+F  S
Sbjct: 35  KEPNDSSSTRASMIRYRA-----LAMVSPSPLPSSSSSSSSSSSSSSFSSNPNFITF--S 94

Query: 66  PSFPIPRPSLSFPSLLSAFL---HKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASL 125
            SFP+ +PSL  PSLLS+FL    K  RSMT+S CFSG +   PI S+   +E+ +S SL
Sbjct: 95  SSFPVLKPSLPLPSLLSSFLSALRKHTRSMTYSRCFSGSTTAVPIPSACH-AEETDSVSL 154

Query: 126 VVVSFYKFADFPDHAEFRLPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIQ 185
           VVVSFYKFADFPDHA+FR PLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFI+
Sbjct: 155 VVVSFYKFADFPDHAQFRQPLKKLCEELRVSGGIILAPEGINGSICGSRESVQRVLGFIE 214

Query: 186 SDERLKGLRQVETPVSPEEEAIHNGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPV 245
           SDERLKGLRQVETPVSPEEEAIH+GH SSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPV
Sbjct: 215 SDERLKGLRQVETPVSPEEEAIHHGHISSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPV 274

Query: 246 ISPVERVGQYVKPRDWNALISDPDTVVIDVRNHYETRIGKFKTAVDPCTTAFRDFPAWVE 305
           ISPVERVG+YVKPRDWN LISDPDTVVIDVRNHYETRIGKFK+AVDPCTTAFRDFP+WVE
Sbjct: 275 ISPVERVGKYVKPRDWNKLISDPDTVVIDVRNHYETRIGKFKSAVDPCTTAFRDFPSWVE 334

Query: 306 DKLQLPKLDCKHSNQGGEE-TVNPKESSPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHL 365
           DK Q+ K D KHSNQG EE  VNP++++PPRVAMYCTGGIRCEKASSYLLSKGFKEVYHL
Sbjct: 335 DKFQVSKSDRKHSNQGVEEDAVNPEQNAPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHL 394

Query: 366 EGGILKYLEEVPKSESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEW 425
           EGGILKYLEEVPK+ESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEW
Sbjct: 395 EGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEW 454

Query: 426 EYGVSCPYCFSTKSEEEKERARARQRQFETWGIIGGPDKGRRPTQNDDNVSHPNSV 473
           EYGVSCPYC+STKSEEEKERARARQRQFETWGIIGGPDKGR P  ++++V+  NS+
Sbjct: 455 EYGVSCPYCYSTKSEEEKERARARQRQFETWGIIGGPDKGRSPNPSNNSVNLSNSI 502

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q1JPN04.3e-16365.24Rhodanese-like domain-containing protein 7 OS=Arabidopsis thaliana OX=3702 GN=ST... [more]
Q1GYV14.0e-8447.11tRNA uridine(34) hydroxylase OS=Methylobacillus flagellatus (strain KT / ATCC 51... [more]
Q92LX65.7e-8347.55tRNA uridine(34) hydroxylase OS=Rhizobium meliloti (strain 1021) OX=266834 GN=tr... [more]
B2IVX79.8e-8346.50tRNA uridine(34) hydroxylase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 7310... [more]
Q8YZS39.8e-8346.65tRNA uridine(34) hydroxylase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2... [more]
Match NameE-valueIdentityDescription
XP_004146431.20.099.78rhodanese-like domain-containing protein 7 [Cucumis sativus][more]
XP_016902023.14.58e-30089.72PREDICTED: LOW QUALITY PROTEIN: rhodanese-like domain-containing protein 7 [Cucu... [more]
KAE8648056.13.12e-291100.00hypothetical protein Csa_005784 [Cucumis sativus][more]
XP_038892558.11.17e-28486.40rhodanese-like domain-containing protein 7 [Benincasa hispida][more]
KAG7031505.11.51e-28284.90Rhodanese-like domain-containing protein 7, partial [Cucurbita argyrosperma subs... [more]
Match NameE-valueIdentityDescription
A0A0A0KM630.099.78Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G223080 P... [more]
A0A1S4E1C52.22e-30089.72LOW QUALITY PROTEIN: rhodanese-like domain-containing protein 7 OS=Cucumis melo ... [more]
A0A6J1FRM38.11e-28084.03rhodanese-like domain-containing protein 7 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1JDN92.07e-27485.59rhodanese-like domain-containing protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1CYE53.15e-27181.30rhodanese-like domain-containing protein 7 OS=Momordica charantia OX=3673 GN=LOC... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001763Rhodanese-like domainSMARTSM00450rhod_4coord: 14..135
e-value: 2.6E-19
score: 80.2
IPR001763Rhodanese-like domainPFAMPF00581Rhodanesecoord: 21..131
e-value: 7.2E-5
score: 23.3
IPR001763Rhodanese-like domainPROSITEPS50206RHODANESE_3coord: 24..138
score: 16.666222
IPR036873Rhodanese-like domain superfamilyGENE3D3.40.250.10coord: 1..174
e-value: 7.1E-52
score: 177.0
IPR036873Rhodanese-like domain superfamilySUPERFAMILY52821Rhodanese/Cell cycle control phosphatasecoord: 3..164
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 202..233
NoneNo IPR availablePANTHERPTHR43268:SF3THIOSULFATE SULFURTRANSFERASE/RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2coord: 2..224
NoneNo IPR availableCDDcd01518RHOD_YceAcoord: 11..131
e-value: 1.6332E-53
score: 165.831
IPR020936tRNA uridine(34) hydroxylasePANTHERPTHR43268THIOSULFATE SULFURTRANSFERASE/RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2coord: 2..224

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G17487.T1Cucsat.G17487.T1mRNA
Cucsat.G17487.T5Cucsat.G17487.T5mRNA
Cucsat.G17487.T9Cucsat.G17487.T9mRNA
Cucsat.G17487.T4Cucsat.G17487.T4mRNA
Cucsat.G17487.T8Cucsat.G17487.T8mRNA
Cucsat.G17487.T3Cucsat.G17487.T3mRNA
Cucsat.G17487.T2Cucsat.G17487.T2mRNA
Cucsat.G17487.T7Cucsat.G17487.T7mRNA
Cucsat.G17487.T6Cucsat.G17487.T6mRNA
Cucsat.G17487.T12Cucsat.G17487.T12mRNA
Cucsat.G17487.T11Cucsat.G17487.T11mRNA