Cucsat.G16997 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G16997
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	RING-type E3 ubiquitin transferase
Location	ctg2465: 276312 .. 277841 (-)
RNA-Seq Expression	Cucsat.G16997
Synteny	Cucsat.G16997

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTTACAACTTCCCAACAACTCTTTTCTCTCATCTCTCCCTCTCTTACAACTTTTTTCTTCCTCAATGGCAGAATTAGATCAAGTACTTGACCCTCCTCCCTATTTTCTCTGTCCCATCTCCTTCCAAATCATGAAAGATCCCGTTACAGTCGCCTCTGGCATAACCTACGACCGTGAAAGCATCGAGAAATGGTTGCTTTCTCACAAACACAACACATGCCCCGTCTCACACATAGTTTTATCCCACTTCCACATCACTCCCAACCATACCTTAAGACGAGTCATTCAAGCTTGGTGCACTCTCAATGCTTCTAAAGGCGTAGAGAGGATACCCACGCCGAAGCCCCCAGTCGACCGGGAGCAAGTCGTTCGGATTCTTGCCCATGCTAACTTATCTCCATTCTCTCAAAAGAATTCTCTTTGTCGCCTTCGATCTATTGCTACGGCAAATGAAAGTAATAAACGTTGCATGGAATCTGCTGGAGTTGTGGAGTTCTTGGCTGGGGTTGTGTGTAATAATTCTACCACCAACATGGAGTATGGCTTAGAAGGTAATATTCAAAACTCAAAACAAATTTAAAGTTTCTTATTTTGCATACCCCTCATTGCCAATAATAAATGTGACGTTATAATGTATAATATATATAAGGTATGAGAATTTAATAATATTGGTTATTTAATTTATGCTTATTTGATGATGCAGATTACGCGTTTGACGACATGTCCGCAAATTCAGCCGATGAGGCTTTGCTCATTCTTCACAAACTCCAAGTCTCCGAACCCTCTCTAAAATTTCTCCTCTCCAACAATGGCGGCATCTTCGTCTCCACTTTAACCAAAATCTTACAAAACCGAAGCTATTCCTCTAGATCATATTCAGTAATGCTTCTTAATTCAATGTTCGAAGTCGCAGATCAGATTCAAATTCAAAATCTCACCGCCGATTTCTTCACCGAAATCATCCAAATCCTCAAAGATCAGATATCCAAACAAGCCTCCAAATCGGCATTGAAATTGCTAATCGCCGTCTCTTCTAGTTTCAGAAACAGAGTAAAGGCGGTTCAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCATCGGAGAATAAGCGTTTGTGCGAAATGATTCTCGTACTGCTGGATCTGCTTTGCAGCTGTGCCGATGGCAGGGCGGAGCTGTTGAATCACGCGGCGGGGATCGCCGTGGTGTCGAAGAAGATTCTGAGAGTTTCGTCGGTTGGAAGTGAGAAAGCGGTTGGGATCTTGTGGTCTGTTGCGAAATTCTCGGGGAGTCAAAGCGTGGTTCAGGAAATGGTGCGGATTGGTGTTGTGACGAAGCTTTGTTTTGTGCTTCAGGTGGTGGGGGCCGGAGTGAAAGCGAAGGAGAAAGCTAAAGAGATTTTGAAGCTTCATGGTCGGACATGGCGGAATTCCTCGTGTTTGCCTTCGACTTTGCGATCAGCTTATCCACAATAACATTATTAGCGTTTTCTTTTCTTCTCTTTACTTGTTT

Coding sequence (CDS)

CTTTTACAACTTCCCAACAACTCTTTTCTCTCATCTCTCCCTCTCTTACAACTTTTTTCTTCCTCAATGGCAGAATTAGATCAAGTACTTGACCCTCCTCCCTATTTTCTCTGTCCCATCTCCTTCCAAATCATGAAAGATCCCGTTACAGTCGCCTCTGGCATAACCTACGACCGTGAAAGCATCGAGAAATGGTTGCTTTCTCACAAACACAACACATGCCCCGTCTCACACATAGTTTTATCCCACTTCCACATCACTCCCAACCATACCTTAAGACGAGTCATTCAAGCTTGGTGCACTCTCAATGCTTCTAAAGGCGTAGAGAGGATACCCACGCCGAAGCCCCCAGTCGACCGGGAGCAAGTCGTTCGGATTCTTGCCCATGCTAACTTATCTCCATTCTCTCAAAAGAATTCTCTTTGTCGCCTTCGATCTATTGCTACGGCAAATGAAAGTAATAAACGTTGCATGGAATCTGCTGGAGTTGTGGAGTTCTTGGCTGGGGTTGTGTGTAATAATTCTACCACCAACATGGAGTATGGCTTAGAAGATTACGCGTTTGACGACATGTCCGCAAATTCAGCCGATGAGGCTTTGCTCATTCTTCACAAACTCCAAGTCTCCGAACCCTCTCTAAAATTTCTCCTCTCCAACAATGGCGGCATCTTCGTCTCCACTTTAACCAAAATCTTACAAAACCGAAGCTATTCCTCTAGATCATATTCAGTAATGCTTCTTAATTCAATGTTCGAAGTCGCAGATCAGATTCAAATTCAAAATCTCACCGCCGATTTCTTCACCGAAATCATCCAAATCCTCAAAGATCAGATATCCAAACAAGCCTCCAAATCGGCATTGAAATTGCTAATCGCCGTCTCTTCTAGTTTCAGAAACAGAGTAAAGGCGGTTCAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGATTCATCGGAGAATAAGCGTTTGTGCGAAATGATTCTCGTACTGCTGGATCTGCTTTGCAGCTGTGCCGATGGCAGGGCGGAGCTGTTGAATCACGCGGCGGGGATCGCCGTGGTGTCGAAGAAGATTCTGAGAGTTTCGTCGGTTGGAAGTGAGAAAGCGGTTGGGATCTTGTGGTCTGTTGCGAAATTCTCGGGGAGTCAAAGCGTGGTTCAGGAAATGGTGCGGATTGGTGTTGTGACGAAGCTTTGTTTTGTGCTTCAGGTGGTGGGGGCCGGAGTGAAAGCGAAGGAGAAAGCTAAAGAGATTTTGAAGCTTCATGGTCGGACATGGCGGAATTCCTCGTGTTTGCCTTCGACTTTGCGATCAGCTTATCCACAATAA

Protein sequence

LLQLPNNSFLSSLPLLQLFSSSMAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYPQ

Homology

[Close]

BLAST of Cucsat.G16997 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.5e-112
Identity = 221/435 (50.80%), Postives = 300/435 (68.97%), Query Frame = 0


Query: 26  LDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFH 85
           +DQ ++ P +FLCPIS  IMKDPV V++GITYDRESIEKWL S K N+CPV+  V++   
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 86  ITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLCRLR 145
           +TPNHTLRR+IQ+WCTLNAS G+ERIPTPKPP+ + ++ +++  ++ S  +Q   L RLR
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 120

Query: 146 SIATANESNKRCMESAGVVEFLAGVVCNN-----------STTNMEYGLEDYAFD---DM 205
            I + N +NKRC+E+A V EFLA +V N+           S++N+    +    +   D 
Sbjct: 121 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 180

Query: 206 SANSADEALLILHKLQVSEPSLKFLLSNNGGI-FVSTLTKILQNRSYSSRSYSVMLLNSM 265
           S +  DEAL +L+ L  SE +LK LL+N  G   V TLTKI+Q   Y SR+Y+ +LL  +
Sbjct: 181 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 240

Query: 266 FEVADQIQIQNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVP 325
            EVAD +QI  L  + F E+IQIL DQIS +A++SA+++L+      RNR KAV+ G + 
Sbjct: 241 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 300

Query: 326 VLIDLLLDLDSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGS 385
           ++I+LL+D   S  +R  EM +V+LD+LC CA+GRAE LNH A IAVVSKKILRVS + S
Sbjct: 301 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 360

Query: 386 EKAVGILWSVAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTW 445
           E+AV +L SV +F  + S++QEM+++GVV KLC VLQ V  G K KEKAKE+LKLH R W
Sbjct: 361 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVW 420

[Close]

BLAST of Cucsat.G16997 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.3e-108
Identity = 210/421 (49.88%), Postives = 292/421 (69.36%), Query Frame = 0


Query: 26  LDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFH 85
           +D+ ++ PP+FLCPIS +IMKDPV V++GITYDR+SIEKWL + K N+CPV+   ++   
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 86  ITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLCRLR 145
           +TPNHTLRR+IQ+WCTLNAS GVERIPTP+PP+ + ++ +++  +  S  +Q   L RLR
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 125

Query: 146 SIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLILHK 205
            I + N +NKRC+E+AGV EFLA +V N               D  + +  DEAL +L+ 
Sbjct: 126 QIVSENATNKRCLEAAGVPEFLANIVSN---------------DSENGSLTDEALNLLYH 185

Query: 206 LQVSEPSLKFLLSN-NGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTA 265
           L+ SE  LK LL+N      V +LTKI+Q   Y SR Y+ +LL ++ EVAD +Q   L  
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP 245

Query: 266 DFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSEN 325
           + FTE++QIL D+IS++A+K+A+ +L+ +    RNR KAV+AG + V+I+LL+D   +  
Sbjct: 246 EVFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSE 305

Query: 326 KRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAKFS 385
           +R  EM +V+LDLLC CA+GRAE LNH A IAVV KKILRVS   S++AV +L SV +F 
Sbjct: 306 RRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFC 365

Query: 386 GSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAY 445
            + +++ EM+++GVV KLC VLQ V  G K KEKAKE+LKLH R W++S CLP  +  AY
Sbjct: 366 ATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAY 410

[Close]

BLAST of Cucsat.G16997 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 4.5e-61
Identity = 169/459 (36.82%), Postives = 245/459 (53.38%), Query Frame = 0


Query: 23  MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 82
           M + ++ ++ P YF+CPIS +IMKDPVT  SGITYDR++I KWL   K  +CPV+   L 
Sbjct: 1   MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 60

Query: 83  -HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQ---- 142
               +TPNH LRR+IQ WC  N ++GV RI TP+ P  +  VV  +   NL  F Q    
Sbjct: 61  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEI--KNLKKFGQEALG 120

Query: 143 -KNSLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSA 202
            + +L +L  +A  + +N+R M   GV + L   V    T+  E G              
Sbjct: 121 REETLQKLEVLA-MDGNNRRLMCECGVHKSLILFVV-KCTSEDEDGRR-------RIKGL 180

Query: 203 DEALLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQ 262
           DE+L +LH + +     K +L  N  + + +LT +L    + S++Y+++LL ++ E    
Sbjct: 181 DESLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSS 240

Query: 263 IQIQNLTADFFTEIIQILKDQIS-----------------------------------KQ 322
             ++ L  + F  II  LKD ++                                   KQ
Sbjct: 241 HIVERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQ 300

Query: 323 ASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENKRLCEMILVLLDLLCSC 382
           A  +AL +L+  SS  RNR   V  GAV  LI+  L++  +  KR+ E++L +L  LC C
Sbjct: 301 AVTAALMILLETSSWSRNRSLLVDLGAVSELIE--LEISYTGEKRITELMLGVLSRLCCC 360

Query: 383 ADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAKFSGSQSVVQEMVRIGVVTK 441
           A+GRAE+L H  GIAVV+K++LRVS    ++A+ IL +V+KFS    VV+EMV +G V K
Sbjct: 361 ANGRAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEK 420

[Close]

BLAST of Cucsat.G16997 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.0e-44
Identity = 139/416 (33.41%), Postives = 218/416 (52.40%), Query Frame = 0


Query: 26  LDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLSHFH 85
           LD  +  P +F CPIS ++M+DPVTV +G TYDR SIE W+    + TCPV+   LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFT 67

Query: 86  ITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHA------NLSPFSQKN 145
           + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A      ++S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 127

Query: 146 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 205
           +L RLR  A  ++ N+  + +    E L  ++ + +T               S+    E+
Sbjct: 128 ALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETT---------------SSELVSES 187

Query: 206 LLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 265
           L +L  L ++EP+    +S++ G  V  LT++L + S  +R  +  L+  +        +
Sbjct: 188 LALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL 247

Query: 266 QNLTAD---FFTEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLID- 325
           +   ++    F  ++ +L++ I S++A K  +K L A+ S    R  A+ AGA  +LID 
Sbjct: 248 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDR 307

Query: 326 LLLDLDSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAV 385
           L  D D  + +R     L  ++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 308 LAADFDRCDTER----ALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAA 367

Query: 386 GILWSVAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTW 431
           G L  +A  +  +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W
Sbjct: 368 GAL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSW 400

[Close]

BLAST of Cucsat.G16997 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 9.3e-43
Identity = 136/428 (31.78%), Postives = 220/428 (51.40%), Query Frame = 0


Query: 22  SMAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVL 81
           ++  LD  +  P +F CPIS  +M DPVT+++G TYDR SI+ W ++  + TCPV+ + L
Sbjct: 4   NLEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSW-IAMGNTTCPVTRVAL 63

Query: 82  SHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHA------NLSPF 141
           S F + PNHTLRR+IQ WC  N S GVERIPTPK P D   V  +L+ A      ++S  
Sbjct: 64  SDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVR 123

Query: 142 SQKNSLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANS 201
           S+  ++ RLR +A  +E N+  +      E L  ++             D     +S+  
Sbjct: 124 SRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRIL-----------FADIETTSLSSEL 183

Query: 202 ADEALLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVAD 261
             E+L +L  L ++E   + + S+     V  +T++L + S   R  +  L+  +   A 
Sbjct: 184 VSESLALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAK 243

Query: 262 QIQIQNLTA---DFFTEIIQILKDQI-SKQASKSALKLLIAVSSSFRNRVKAVQAGAVPV 321
            + ++ + +     F  ++ +LK+ I S++A K  +K + A+    + R  A+ AGA  +
Sbjct: 244 SMDLKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGI 303

Query: 322 LID-LLLDLDSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGS 381
           LID L  D D  + +R     L  ++LLC   +G A    HA  + ++ K ILRVS   +
Sbjct: 304 LIDRLAADFDRCDTER----GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRAT 363

Query: 382 EKAVGILWSVAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTW 439
           E A G L  +A  +  +    E    G+VT+L  ++Q      +AK KA+ +LKL   +W
Sbjct: 364 EYAAGAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSW 410

[Close]

BLAST of Cucsat.G16997 vs. NCBI nr
Match: XP_011653475.1 (E3 ubiquitin-protein ligase PUB22 [Cucumis sativus] >KGN53961.1 hypothetical protein Csa_021573 [Cucumis sativus])

HSP 1 Score: 809 bits (2089), Expect = 2.00e-294
Identity = 422/424 (99.53%), Postives = 423/424 (99.76%), Query Frame = 0


Query: 23  MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 82
           MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS
Sbjct: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60

Query: 83  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 142
           HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC
Sbjct: 61  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 120

Query: 143 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI 202
           RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI
Sbjct: 121 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI 180

Query: 203 LHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNL 262
           LHKLQVSE SLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMF+VADQIQIQNL
Sbjct: 181 LHKLQVSESSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFQVADQIQIQNL 240

Query: 263 TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS 322
           TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS
Sbjct: 241 TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS 300

Query: 323 ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK 382
           ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK
Sbjct: 301 ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK 360

Query: 383 FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS 442
           FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS
Sbjct: 361 FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS 420

Query: 443 AYPQ 446
           AYPQ
Sbjct: 421 AYPQ 424

[Close]

BLAST of Cucsat.G16997 vs. NCBI nr
Match: XP_008441961.1 (PREDICTED: E3 ubiquitin-protein ligase PUB22 [Cucumis melo] >KAA0060143.1 E3 ubiquitin-protein ligase PUB22 [Cucumis melo var. makuwa] >TYK08456.1 E3 ubiquitin-protein ligase PUB22 [Cucumis melo var. makuwa])

HSP 1 Score: 756 bits (1953), Expect = 1.13e-273
Identity = 397/427 (92.97%), Postives = 409/427 (95.78%), Query Frame = 0


Query: 23  MAELD---QVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHI 82
           MAELD   QV+DPPPYFLCPIS QIMKDPVT+ASGITYDRESIEKWL S KH TCPVSHI
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 83  VLSHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKN 142
           VLS F +TPNHTLRRVIQAWCT+NASKGVERIPTPKPPVDREQVVRILA A LSPFSQKN
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 143 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 202
            LCRLRSIA ANESNKRCMESAGVVEFLAGVVCN STTNMEYGLEDYAFDDMSANSADEA
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCN-STTNMEYGLEDYAFDDMSANSADEA 180

Query: 203 LLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 262
           L+ILHKLQ+SE SLKFLLSNNGGIF+STLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI
Sbjct: 181 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240

Query: 263 QNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 322
           QNLTADFF EIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL
Sbjct: 241 QNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300

Query: 323 DSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWS 382
           DSSENKRLCEMILVLLD LC CADGRAELLNHAAGIAVVSKKILR+S+VGSEKAVGILWS
Sbjct: 301 DSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWS 360

Query: 383 VAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 442
           +A+FSGSQSVVQEMVRIGVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST
Sbjct: 361 IARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420

Query: 443 LRSAYPQ 446
           LRSAYPQ
Sbjct: 421 LRSAYPQ 426

[Close]

BLAST of Cucsat.G16997 vs. NCBI nr
Match: XP_038882772.1 (E3 ubiquitin-protein ligase PUB22-like [Benincasa hispida])

HSP 1 Score: 682 bits (1761), Expect = 1.82e-244
Identity = 363/425 (85.41%), Postives = 391/425 (92.00%), Query Frame = 0


Query: 23  MAELDQ-VLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVL 82
           MAELDQ V+DPPP+FLCPIS QIMKDPVT+ASGITYDRE+IEKWL S KH TCPVS +VL
Sbjct: 1   MAELDQEVIDPPPFFLCPISLQIMKDPVTIASGITYDRENIEKWLFSDKHKTCPVSKVVL 60

Query: 83  SHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSL 142
             F ITPNHTLRRVIQAWCT NA+ GVERIPTPKPPVDR+QVVRILA A LSPFSQ+N L
Sbjct: 61  LDFDITPNHTLRRVIQAWCTANATSGVERIPTPKPPVDRKQVVRILADAKLSPFSQQNCL 120

Query: 143 CRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALL 202
            RLRSIA ANE+NKRCME+AGVVEFLAG+VC+ STTN+E GLEDYAFDDMSAN  DEAL 
Sbjct: 121 RRLRSIAAANETNKRCMEAAGVVEFLAGLVCS-STTNVESGLEDYAFDDMSANLGDEALS 180

Query: 203 ILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQN 262
           IL+KLQ+SE SLKFLLSNNGGIF+STLTKILQNRSYSSR+YS+MLLNSMFEVADQIQI N
Sbjct: 181 ILYKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRAYSIMLLNSMFEVADQIQILN 240

Query: 263 LTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDS 322
           LT DFF EI+QILKDQISKQASKSALKLLI V SS RNRVKAV+AGAVPVL+DLLLDLDS
Sbjct: 241 LTTDFFIEIVQILKDQISKQASKSALKLLIGVCSSIRNRVKAVEAGAVPVLVDLLLDLDS 300

Query: 323 SENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVA 382
           SE KRL EMILV+LDLLC CADGRA LLNHAAGIAVVSKKILRVSS+GSEKAVGIL SVA
Sbjct: 301 SE-KRLNEMILVVLDLLCGCADGRAGLLNHAAGIAVVSKKILRVSSLGSEKAVGILCSVA 360

Query: 383 KFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLR 442
           +FS SQSV+QEMVR+GVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS+LR
Sbjct: 361 RFSASQSVLQEMVRLGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSSLR 420

Query: 443 SAYPQ 446
           SAYPQ
Sbjct: 421 SAYPQ 423

[Close]

BLAST of Cucsat.G16997 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 649 bits (1673), Expect = 4.34e-231
Identity = 341/425 (80.24%), Postives = 380/425 (89.41%), Query Frame = 0


Query: 23  MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 82
           MAELDQ LDPPP+FLCPIS QIMKDPVTVASGITYDRESIE+WL S +H TCPV+ +VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 83  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 142
            F ITPNHT+RRVIQAWCT+NA KG+ERIPTPKPP+D +QVVRILA A LSPFSQ+N L 
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 143 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAF--DDMSANSADEAL 202
           RLRSIA A+ESNKRCME+AG VEFLAG+VCN+  TN+E+ LEDYA+  DD  AN  DEAL
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNS--TNVEFSLEDYAYLIDDQFANLGDEAL 180

Query: 203 LILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 262
            IL+KLQ+SE  LKFLL+NNGGIFVSTLTKILQN +Y SR+YSVMLL SMFEVADQIQI 
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 263 NLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 322
           +L  DFF EI+QILKDQISKQASKSALKLLI + SS RNRVKAVQAGA+PVLIDLLLDLD
Sbjct: 241 SLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD 300

Query: 323 SSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSV 382
           SSE KR CEMILV LDLLC CADGRAE+LNHAAGIAVVSKKILRVS++GSEKAVGIL S+
Sbjct: 301 SSE-KRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSI 360

Query: 383 AKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 442
           A+FS S+SVVQEM+R+GVVTKLCF+LQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 420

Query: 443 RSAYP 445
           RS++P
Sbjct: 421 RSSFP 422

[Close]

BLAST of Cucsat.G16997 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645 bits (1665), Expect = 7.16e-230
Identity = 341/425 (80.24%), Postives = 378/425 (88.94%), Query Frame = 0


Query: 23  MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 82
           MAELDQ LDPPP+FLCPIS QIMKDPVTVASGITYDRESIEKWL S +H TCPV+ +VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60

Query: 83  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 142
            F ITPNHTLRRVIQAWCT+NA KG+ERIPTPKPPVD +QVVRILA A LSPFSQ+N L 
Sbjct: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120

Query: 143 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAF--DDMSANSADEAL 202
           RLRSIA  +ESNKRCME+AG VEFLAG+VCN+  TN+E+  EDYA+  DD  AN  DEAL
Sbjct: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNS--TNVEFSSEDYAYLIDDQFANLGDEAL 180

Query: 203 LILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 262
            IL+KLQ+SE  LKFLL+NNGGIFVSTLTKILQN +Y SR+YSVMLL SMFEVADQIQI 
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 263 NLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 322
           +L  DFF EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPV+IDLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLD 300

Query: 323 SSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSV 382
           SSE KR CEMILV LDLLC CADGRAE+LNHAAGIAVVSKKILRVS++GSEKAVGIL S+
Sbjct: 301 SSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSI 360

Query: 383 AKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 442
           A+FS S+SVVQEM+R+GVVTKLCF+LQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 420

Query: 443 RSAYP 445
           RS++P
Sbjct: 421 RSSFP 422

[Close]

BLAST of Cucsat.G16997 vs. ExPASy TrEMBL
Match: A0A0A0L1F5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G194780 PE=4 SV=1)

HSP 1 Score: 809 bits (2089), Expect = 9.70e-295
Identity = 422/424 (99.53%), Postives = 423/424 (99.76%), Query Frame = 0


Query: 23  MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 82
           MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS
Sbjct: 1   MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 60

Query: 83  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 142
           HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC
Sbjct: 61  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 120

Query: 143 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI 202
           RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI
Sbjct: 121 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEALLI 180

Query: 203 LHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNL 262
           LHKLQVSE SLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMF+VADQIQIQNL
Sbjct: 181 LHKLQVSESSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFQVADQIQIQNL 240

Query: 263 TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS 322
           TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS
Sbjct: 241 TADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSS 300

Query: 323 ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK 382
           ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK
Sbjct: 301 ENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSVAK 360

Query: 383 FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS 442
           FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS
Sbjct: 361 FSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRS 420

Query: 443 AYPQ 446
           AYPQ
Sbjct: 421 AYPQ 424

[Close]

BLAST of Cucsat.G16997 vs. ExPASy TrEMBL
Match: A0A5A7UVZ1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00730 PE=4 SV=1)

HSP 1 Score: 756 bits (1953), Expect = 5.49e-274
Identity = 397/427 (92.97%), Postives = 409/427 (95.78%), Query Frame = 0


Query: 23  MAELD---QVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHI 82
           MAELD   QV+DPPPYFLCPIS QIMKDPVT+ASGITYDRESIEKWL S KH TCPVSHI
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 83  VLSHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKN 142
           VLS F +TPNHTLRRVIQAWCT+NASKGVERIPTPKPPVDREQVVRILA A LSPFSQKN
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 143 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 202
            LCRLRSIA ANESNKRCMESAGVVEFLAGVVCN STTNMEYGLEDYAFDDMSANSADEA
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCN-STTNMEYGLEDYAFDDMSANSADEA 180

Query: 203 LLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 262
           L+ILHKLQ+SE SLKFLLSNNGGIF+STLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI
Sbjct: 181 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240

Query: 263 QNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 322
           QNLTADFF EIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL
Sbjct: 241 QNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300

Query: 323 DSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWS 382
           DSSENKRLCEMILVLLD LC CADGRAELLNHAAGIAVVSKKILR+S+VGSEKAVGILWS
Sbjct: 301 DSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWS 360

Query: 383 VAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 442
           +A+FSGSQSVVQEMVRIGVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST
Sbjct: 361 IARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420

Query: 443 LRSAYPQ 446
           LRSAYPQ
Sbjct: 421 LRSAYPQ 426

[Close]

BLAST of Cucsat.G16997 vs. ExPASy TrEMBL
Match: A0A1S3B4K2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 SV=1)

HSP 1 Score: 756 bits (1953), Expect = 5.49e-274
Identity = 397/427 (92.97%), Postives = 409/427 (95.78%), Query Frame = 0


Query: 23  MAELD---QVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHI 82
           MAELD   QV+DPPPYFLCPIS QIMKDPVT+ASGITYDRESIEKWL S KH TCPVSHI
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 83  VLSHFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKN 142
           VLS F +TPNHTLRRVIQAWCT+NASKGVERIPTPKPPVDREQVVRILA A LSPFSQKN
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 143 SLCRLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAFDDMSANSADEA 202
            LCRLRSIA ANESNKRCMESAGVVEFLAGVVCN STTNMEYGLEDYAFDDMSANSADEA
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCN-STTNMEYGLEDYAFDDMSANSADEA 180

Query: 203 LLILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 262
           L+ILHKLQ+SE SLKFLLSNNGGIF+STLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI
Sbjct: 181 LIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQI 240

Query: 263 QNLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 322
           QNLTADFF EIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL
Sbjct: 241 QNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDL 300

Query: 323 DSSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWS 382
           DSSENKRLCEMILVLLD LC CADGRAELLNHAAGIAVVSKKILR+S+VGSEKAVGILWS
Sbjct: 301 DSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWS 360

Query: 383 VAKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 442
           +A+FSGSQSVVQEMVRIGVVTKLCF+LQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST
Sbjct: 361 IARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPST 420

Query: 443 LRSAYPQ 446
           LRSAYPQ
Sbjct: 421 LRSAYPQ 426

[Close]

BLAST of Cucsat.G16997 vs. ExPASy TrEMBL
Match: A0A6J1IKG7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 PE=4 SV=1)

HSP 1 Score: 649 bits (1673), Expect = 2.10e-231
Identity = 341/425 (80.24%), Postives = 380/425 (89.41%), Query Frame = 0


Query: 23  MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 82
           MAELDQ LDPPP+FLCPIS QIMKDPVTVASGITYDRESIE+WL S +H TCPV+ +VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 83  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 142
            F ITPNHT+RRVIQAWCT+NA KG+ERIPTPKPP+D +QVVRILA A LSPFSQ+N L 
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 143 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAF--DDMSANSADEAL 202
           RLRSIA A+ESNKRCME+AG VEFLAG+VCN+  TN+E+ LEDYA+  DD  AN  DEAL
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNS--TNVEFSLEDYAYLIDDQFANLGDEAL 180

Query: 203 LILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 262
            IL+KLQ+SE  LKFLL+NNGGIFVSTLTKILQN +Y SR+YSVMLL SMFEVADQIQI 
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 263 NLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 322
           +L  DFF EI+QILKDQISKQASKSALKLLI + SS RNRVKAVQAGA+PVLIDLLLDLD
Sbjct: 241 SLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD 300

Query: 323 SSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSV 382
           SSE KR CEMILV LDLLC CADGRAE+LNHAAGIAVVSKKILRVS++GSEKAVGIL S+
Sbjct: 301 SSE-KRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSI 360

Query: 383 AKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 442
           A+FS S+SVVQEM+R+GVVTKLCF+LQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 420

Query: 443 RSAYP 445
           RS++P
Sbjct: 421 RSSFP 422

[Close]

BLAST of Cucsat.G16997 vs. ExPASy TrEMBL
Match: A0A6J1GGQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790 PE=4 SV=1)

HSP 1 Score: 640 bits (1650), Expect = 6.65e-228
Identity = 338/425 (79.53%), Postives = 378/425 (88.94%), Query Frame = 0


Query: 23  MAELDQVLDPPPYFLCPISFQIMKDPVTVASGITYDRESIEKWLLSHKHNTCPVSHIVLS 82
           MAELDQ LDPPP+FLCPIS QIMKDPVTVASGITYDRESIE+WL S +H TCP++ +VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60

Query: 83  HFHITPNHTLRRVIQAWCTLNASKGVERIPTPKPPVDREQVVRILAHANLSPFSQKNSLC 142
           +F ITPNHTLRRVIQAWCT+NA KGVERIPTPKPPVD +QVVRILA A LS FSQ+N L 
Sbjct: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120

Query: 143 RLRSIATANESNKRCMESAGVVEFLAGVVCNNSTTNMEYGLEDYAF--DDMSANSADEAL 202
           RL+SIA A+ESNKRCME+AG VEFLAG+VCN+  TN+E+ LEDYA+  DD  AN   EAL
Sbjct: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNS--TNVEFSLEDYAYLIDDQLANLGFEAL 180

Query: 203 LILHKLQVSEPSLKFLLSNNGGIFVSTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 262
            IL+KLQ+SE  LKFLL+NNGGIFVSTLTKILQN +Y SR+YSVMLL SMFEVADQIQI 
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQIL 240

Query: 263 NLTADFFTEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 322
           +L  DFF EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPVLIDLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD 300

Query: 323 SSENKRLCEMILVLLDLLCSCADGRAELLNHAAGIAVVSKKILRVSSVGSEKAVGILWSV 382
           SSE KR CEMILV LDLLC CADGRAE+LNHAAGIA+VSKKILRVS++GSEKAVGIL S+
Sbjct: 301 SSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSI 360

Query: 383 AKFSGSQSVVQEMVRIGVVTKLCFVLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 442
           A+FS S+SVVQEM+R+GVVTKLCF+LQV GAG KAKEKAKEILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 420

Query: 443 RSAYP 445
           RS++P
Sbjct: 421 RSSFP 422

The following BLAST results are available for this feature:

BLAST of Cucsat.G16997 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SVC6	1.5e-112	50.80	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q84TG3	1.3e-108	49.88	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SF15	4.5e-61	36.82	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	1.0e-44	33.41	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9FXA4	9.3e-43	31.78	U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2...	[more]

BLAST of Cucsat.G16997 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011653475.1	2.00e-294	99.53	E3 ubiquitin-protein ligase PUB22 [Cucumis sativus] >KGN53961.1 hypothetical pro...	[more]
XP_008441961.1	1.13e-273	92.97	PREDICTED: E3 ubiquitin-protein ligase PUB22 [Cucumis melo] >KAA0060143.1 E3 ubi...	[more]
XP_038882772.1	1.82e-244	85.41	E3 ubiquitin-protein ligase PUB22-like [Benincasa hispida]	[more]
XP_022978192.1	4.34e-231	80.24	E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima]	[more]
XP_023543358.1	7.16e-230	80.24	E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G16997 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1F5	9.70e-295	99.53	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G194780 PE...	[more]
A0A5A7UVZ1	5.49e-274	92.97	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B4K2	5.49e-274	92.97	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 ...	[more]
A0A6J1IKG7	2.10e-231	80.24	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 P...	[more]
A0A6J1GGQ8	6.65e-228	79.53	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 35..99 e-value: 1.3E-31 score: 121.0
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 33..101 e-value: 4.1E-21 score: 74.9
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 31..106 score: 39.170525
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 20..111 e-value: 1.3E-27 score: 97.6
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 120..427 e-value: 3.3E-14 score: 54.5
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 30..445
None	No IPR available	PANTHER	PTHR22849:SF132	E3 UBIQUITIN-PROTEIN LIGASE PUB23	coord: 30..445
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 36..78 e-value: 1.88652E-20 score: 82.2815
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 28..104
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 124..407

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G16997.T1	Cucsat.G16997.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity