Cucsat.G16913 (gene) Cucumber (B10) v3

Overview
NameCucsat.G16913
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionPyruvate kinase
Locationctg24: 2938282 .. 2943012 (-)
RNA-Seq ExpressionCucsat.G16913
SyntenyCucsat.G16913
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGCTATCTATTGCAATTTATTGTAGTGATTTTTTAATTTTTTTAAATTTTCATTTGAAACAAGTTAGAAGTAAATAATTGATAATTTAAGAGAAGAATTTGTTAAAGATTTAGGAAAATTCCAAAAGAAATGGGAAAGGGAGAAAAGGGTTTTGGGTCGGACAGAACTGCAGGAAACAGTGGGTGGCACGTGAGGTTTAGAGGTTGAACCTAAACCACAAGCCAACGCCGGGAAAGTTAGTTGACCGGTAAAGTTCAAATTAATTTATATCTCTTCTTAACACCTAAACACTGTGACAAAAGTCTTCTTCCCATTCATAAATAATTAGGGTTCTTCTCATCGAATATTCTTCTTCTTCTTTCTTGTTAAATCCACCATTGTTCCTTACTCGGCGGACTATACTATTTGAAGCTATCTAAGGCCTCTTCTTGCAGAAGAAGGCTAGATAGTTGCAGAGTTACTTAATCTTCTTTCTTGTTTTTTGTTAATGGCGGAGGTCAATGCTATGATGATTCGTGCTCGCTGTAATTTTATGGTGAGAAACCCTGTGTTGGATGTGAAGCGTTTGGGCTTTCGGATGCCGGTTATGGGTCGCCGAACAGTTTGGTGTAGTCGGAGGAGGATGAAGGGGAGAGTTGGAAATGGAGTGAAGGCGACAATGCAGGTCGATTTGGAGGATGAGGAGAGGTTGAAAAGATTGGAGGGTTTAGAGGCCACTTTAGGGCTTGATATTGTAGCGGAGAGGGAATTGAAGGAGAAGGGGTTTTTAGGGATGAGGAAGACGAAGCTTGTATGCACTGTTGGTCCAGCGTGTAGTGGGATTGCGGATTTGGAGAAGTTAGCGTTGGAAGGTATGAATATTGCGAGATTGAATATGTGTCACAACTCGATGGAGTGGCATTGTGATATGATTAAGAAAATCAAGAAGTTAAATGAGGAGAAAGGATTCTGTATTTCGGTTATGATTGATATTGAAGGAAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCTGTAAAAGTTGAGGTAAATTGTAATTGTCATGTCTCTGTTTTCATTGTTTCCCTTTACTGTAGTCTAAATTCTCTGGTCGTCATTGTTCAGGATGGTTCAATCTGGTTGTTCACTGCTAAGAAATTCGAAGGTTCTCGTCCATTCACTGTGCAAGCGAGCTATGAAGGTTTCTCTGAAGGTGCGTTTTGAATCGTGTATTCTATTTGTGATCGAAGAAATATTTGAATCCGAGAAGCTAATTAGGACATGGTTTTGAAGTTGAAGATCTTGTTTTTACGAATTTGGGCAGGTATCCGAGTGGGTGATTCAATTGTAATTGATGGGGGAATGGCAACCTTTGAAGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAGGTGCACAGACCCAGGTTTATTTCTTCCTCGGGCGAAGTGTAGCTTTTGGAGAGATGGAAGGCTTGTTATGAGAAATCACGAGTCTCCCACGTTGTCAAGTAAGGTAGGAACGTTTTTCCTGTAATATCAATATTCATACTGAGAAATTTATTTTTGATAAATATAATTAGAAGTGCTCACTCTTAAGTTAAACTTTCAAGTATGATGTATTCAAAGATCAAGAACCTAAGATGTTGCATTCTTCGTTTTGGTATTACTATGGAGGAAATCTTATATCCATTATTTTCGAAGTTTTCTTTCTGTGGGATTACAAGTTGGGTCCACTATCTTGAGTCGTGGGGACAGTTTCATGGTTCGACATTTTTTGTTACTCTGGGTTGTCAACATGGCAATGTTTTTACTTGATTCAATATGGGAATAACAGTTTAAGGTTCATTATTTAAGAGTTGGCTTCTTGTTGTGGTTGTCAACACTACGAGTTCAATTTCAAAATTTTTCTTTTTTGGGTGCCAGCATTAAAAGTTTATTCCTGGACATTTTCATCTCTGTCGTGAAGAAAATTTATCTATTTTTATGCACGTAAAACTAATTTTCTTGACAATTGTCTCTCCATTGACGAGGTTGACTTTCTCATATTAATGCAGAGTAAAAGGTCTTGGTATTATGTAATTAAATTTATCTAACCTATCAACTTATATATTTTTAGGCTGATCGATGTATTGGATCTTTCTGATTAATGTCATTTCTTCTAATTAATGTTAATTTTCACATGTGAACTTTTTACAAATTTATAATTCACGAGCCCGAGTATTAAACTATTGAAATAATTTCAGGTTGACATGGAATTCAATTAATATGATGGTGTATTACGTTATTCGGTTTTCCGTTGTGCAGGATTGGTCGGACATTGAATTTGGAATTTCAGAAGGCGTGGACTTCATTGCCCTTTCTTTTGTGAACAGTGCAGATCCTGTCAGGCATCTAAAGAACTACCTTTCAACAAAATCAGCGAAGTAAGCATCGATTCAAGAAGTCAATATTTTACCATGAGTTAGGTCGAAATATCACTATCACTACTATGCATGAACTTTTGATTCCTTGTCTTTTTTTAATTGAATTTTCAACAGCATTTCATAAGATGGATTCCCATGACACTATTGAGGCATTATTTTCCGCAAATTTTGATTACTGAAACAGATTGGGTAATTTTAAAACTTCGCTCAAGTGATGAAGTGTATATTCTCTTTTTCATGCACAGATCTATAAGGGTATTGGCTAAGATTGAAAGCTTGGAGGCCCTTCAAAATCTGGAAGAAATTGTAGAGGCCTCTGATGGAATCATGGTGGCTCGAGGGGACCTTGGGGTTGAAATTCCGCTCGAGCAGATACCAGCAGTGCAAGAGGAAATAACCAGAGTTTGTAGGGAATTGAACAAACCAGTGATCATAGCTTCTCAACTTCTAGAGTCCATGGTTGAATATCCTACCCCAACACGTGCTGAGGTACAAGTGACATGTGCTTTTGATTGTTTTCAATTTTGGACTTGTTTGAAATGACTATCTAAGTATTTAGAAAAGTGTCTTTTTAAGGCTCTTCTTGTGCTCTCCAGAAGGCTGAGATTTCATACTCTTCGTGTTCAATAGGTTGCAGATGTTTCTGAAGCTGTCCGACAATATGCTGATGCGTTGATGCTATCTGGGGAGTCTGCCATAGGATCATATGGACAGAAAGCACTTTCGGTTCTACAGATGGCCAGCAGCCGAATGGAATTATGGAGTCGTGAAGAAAATAGGAACAATTTCCTTCCCCAGCATCAACTTGGAGTGTCATTGCATGATCGCATTGCAGAAGAAATTTGCAATTCTGCAGCTGAATTGGGTAAAACTCACTTTTCTCCACTTTCTTTTTAAACAAAGTCCATCGTATGGGATCGTCAAAATAACATCATAATTCCCTTTTGGCTATGTCTCATGTCTATCGGCTACCTTCTTTATTACGTAATCATGCAAAAGAAAATCGTATGAAATATATTGGCTTGCCATTTTAGTCCATTCTTCTGCGCTTAGTTTCAATGTGGTATTTCTGGTTTAGAAGTATATATCATCAAAATCCTTCAAAAACATATCGGCTGGATGTTTTCACTCATTTCTGTTCTGAGTCACTGTCACACTGTTCTCGATCAAATTTTTAAAAAATCTTTTACTTATTAAGTAATTTAAAAACCTTTGTAAAAGGTAAGAGTAGAGTAATATCGTAACTTCCACAAGTACAGGGCTAGTTTTGAAACAAATGGTCAGTTGGAGGATATTTTCTTATGATTTAACCTTAAAAGTTGTTAACAATTGGTAACAACAGAGAAGTTTTGTGAGAAAAAGCTGGTTGTGTCATGGTGTGGTAGAACTAGAACACTGAGTCTTTGATTCTTAGGTTTGAAGAAGTAAATCAATTACCTTCAAAACAAGAGGTTCTGTTTGTTTGAATGTTGCTAATTGCATTTGGCCCACGTTTCCACTAAAATAAAAACCTCCATATTCTTTGTGGCAGCTAACAGACTTTCAGTAGACGCCATATTTGTGCTCACAAATCAAGGGCATATGGCATCTCTCCTTTCCCGGAACCGCCCAGGGCCGCCAATATTTGCATTAACCGACGATGATAGCACAAGAATGGCTTTGAATCTACAATGGGGAGTGTTTCCTTTGCGAATTGACCTCTCAGAAGACATTGAAGCAAACATCTCAAGAGGGATCGAGGTTGTGAAATCAAAGGGATTGGTAAAACAGGGGGACTCTGTGCTTGTGGTCTCAGAGATCTCTCCAGCTCGAGCTGCGTCAATGGCGTCCCAATCAATTCAGCTCAAAACCATCGTGTAGAACAAAGAAAAAACAGGTCAAGCTTTGTAAGTGAAACTTTATGATTGCATGTATCATATGTGGTGGATATTACACGTGAATGTTGCATCTTATCATTTGTGGCCCTCTGTTTGTAACGACTATTGAAGCCTTTGATAGGTTGTTTATTATTATTTGGAAAATGTAATAACTCTACTCAAATTTGAATTTAGTTTTAATAGTATTGTATTGTTTTGTTTGGTAACCCTTTTATTTTTATTTGTTTGAAAACCATCCTAATAATTATCTTTTTAAATGCATAATTAATCATAATTATTTTAAAAGCTATATTGAACATATAATTTTAAGAAAGTTTAGGATTTAATTACTTTAGCTAGAGTCTATATCGATATAATTCCAAGATTTAAAGCCTAAATTGATTTTAGGTCTAAGTCCTACCCAAATGCTTGACGCCATGAGTAGAGTACGTTAATTATAAGATC

Coding sequence (CDS)

ATGGCGGAGGTCAATGCTATGATGATTCGTGCTCGCTGTAATTTTATGGTGAGAAACCCTGTGTTGGATGTGAAGCGTTTGGGCTTTCGGATGCCGGTTATGGGTCGCCGAACAGTTTGGTGTAGTCGGAGGAGGATGAAGGGGAGAGTTGGAAATGGAGTGAAGGCGACAATGCAGGTCGATTTGGAGGATGAGGAGAGGTTGAAAAGATTGGAGGGTTTAGAGGCCACTTTAGGGCTTGATATTGTAGCGGAGAGGGAATTGAAGGAGAAGGGGTTTTTAGGGATGAGGAAGACGAAGCTTGTATGCACTGTTGGTCCAGCGTGTAGTGGGATTGCGGATTTGGAGAAGTTAGCGTTGGAAGGTATGAATATTGCGAGATTGAATATGTGTCACAACTCGATGGAGTGGCATTGTGATATGATTAAGAAAATCAAGAAGTTAAATGAGGAGAAAGGATTCTGTATTTCGGTTATGATTGATATTGAAGGAAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCTGTAAAAGTTGAGGATGGTTCAATCTGGTTGTTCACTGCTAAGAAATTCGAAGGTTCTCGTCCATTCACTGTGCAAGCGAGCTATGAAGGTTTCTCTGAAGGTATCCGAGTGGGTGATTCAATTGTAATTGATGGGGGAATGGCAACCTTTGAAGTTATTGAAAAGATTGGGAATGATTTAAGCTGCAGGTGCACAGACCCAGGTTTATTTCTTCCTCGGGCGAAGTGTAGCTTTTGGAGAGATGGAAGGCTTGTTATGAGAAATCACGAGTCTCCCACGTTGTCAAGTAAGGATTGGTCGGACATTGAATTTGGAATTTCAGAAGGCGTGGACTTCATTGCCCTTTCTTTTGTGAACAGTGCAGATCCTGTCAGGCATCTAAAGAACTACCTTTCAACAAAATCAGCGAAATCTATAAGGGTATTGGCTAAGATTGAAAGCTTGGAGGCCCTTCAAAATCTGGAAGAAATTGTAGAGGCCTCTGATGGAATCATGGTGGCTCGAGGGGACCTTGGGGTTGAAATTCCGCTCGAGCAGATACCAGCAGTGCAAGAGGAAATAACCAGAGTTTGTAGGGAATTGAACAAACCAGTGATCATAGCTTCTCAACTTCTAGAGTCCATGGTTGAATATCCTACCCCAACACGTGCTGAGGTTGCAGATGTTTCTGAAGCTGTCCGACAATATGCTGATGCGTTGATGCTATCTGGGGAGTCTGCCATAGGATCATATGGACAGAAAGCACTTTCGGTTCTACAGATGGCCAGCAGCCGAATGGAATTATGGAGTCGTGAAGAAAATAGGAACAATTTCCTTCCCCAGCATCAACTTGGAGTGTCATTGCATGATCGCATTGCAGAAGAAATTTGCAATTCTGCAGCTGAATTGGCTAACAGACTTTCAGTAGACGCCATATTTGTGCTCACAAATCAAGGGCATATGGCATCTCTCCTTTCCCGGAACCGCCCAGGGCCGCCAATATTTGCATTAACCGACGATGATAGCACAAGAATGGCTTTGAATCTACAATGGGGAGTGTTTCCTTTGCGAATTGACCTCTCAGAAGACATTGAAGCAAACATCTCAAGAGGGATCGAGGTTGTGAAATCAAAGGGATTGGTAAAACAGGGGGACTCTGTGCTTGTGGTCTCAGAGATCTCTCCAGCTCGAGCTGCGTCAATGGCGTCCCAATCAATTCAGCTCAAAACCATCGTGTAG

Protein sequence

MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQVDLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLALEGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKVEDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPVRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV
Homology
BLAST of Cucsat.G16913 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 4.5e-170
Identity = 291/487 (59.75%), Postives = 374/487 (76.80%), Query Frame = 0

Query: 80  LDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLALEGMNIARLNMCHNSMEWHC 139
           +D V E ELKE GF   R+TKL+CT+GPA  G   LE LA+ GMN+ARLNMCH + +WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 140 DMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKVEDGSIWLFTAKKFEGSRP- 199
            +I+ +++LNEEKGF +++M+D EGS+IH+ D G  +S K EDG +W FT + F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 200 FTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSFWRD 259
            T+  SY+GF+E +RVGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 260 GRLVM-RNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPVRHLKNYLSTKS-AKSI 319
           G LV  RN   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ + HLK+YL+ +S    I
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 320 RVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELNKPVI 379
            V+AKIES+++L NLEEI+ ASDG MVARGDLG +IPLEQ+PA Q+ I +VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 380 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRM 439
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 440 ELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMASLLS 499
           E W REE R+  +P   +G S  D+I+EEICNSAA++AN L VDA+FV T  GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 500 RNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVKQGDS 559
           R RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+++   ++KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 560 VLVVSEI 564
           V+ VS++
Sbjct: 580 VIAVSDM 586

BLAST of Cucsat.G16913 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 1.7e-169
Identity = 287/491 (58.45%), Postives = 380/491 (77.39%), Query Frame = 0

Query: 76  ATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLALEGMNIARLNMCHNSM 135
           +++ +D V E ELKE GF   R+TKLVCT+GPA  G  +LE LA+ GMN+AR+NMCH + 
Sbjct: 83  SSIEVDAVTETELKENGFRSTRRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTR 142

Query: 136 EWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKVEDGSIWLFTAKKFEG 195
           EWH  +I+++++LNEEKGF +++M+D EGS+IH+ D G  SS K EDG IW F+ + ++ 
Sbjct: 143 EWHKSVIERVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDS 202

Query: 196 SRP-FTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCS 255
            RP  T+  +Y+GF+E ++VGD +++DGGM  FEVIEKIG D+ CRCTDPGL LPRA  +
Sbjct: 203 PRPERTINVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLT 262

Query: 256 FWRDGRLVM-RNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPVRHLKNYLSTKSA 315
           FWRDG LV  RN   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ + HLK+Y++ +S 
Sbjct: 263 FWRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSR 322

Query: 316 KS-IRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELN 375
            S I V+AKIES+++L+NLEEI+ ASDG MVARGDLG +IPLEQ+P+ Q+ I +VCR+LN
Sbjct: 323 DSDIAVIAKIESIDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLN 382

Query: 376 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 435
           KPVI+ASQLLESM+EYPTPTRAEVADVSEAVRQ ADALMLSGESA+G Y +KAL+VL+  
Sbjct: 383 KPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSV 442

Query: 436 SSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMA 495
           S R+E W REE  +  +    +G +  D I+EEICNSAA++AN L VDA+FV T  GHMA
Sbjct: 443 SVRIEKWWREEKHHEAMELPAIGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMA 502

Query: 496 SLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVK 555
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  ++D+E+N+++   ++K++G++K
Sbjct: 503 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIK 562

Query: 556 QGDSVLVVSEI 564
            GD V+ VS++
Sbjct: 563 SGDLVIAVSDM 573

BLAST of Cucsat.G16913 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.6e-164
Identity = 280/491 (57.03%), Postives = 377/491 (76.78%), Query Frame = 0

Query: 76  ATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLALEGMNIARLNMCHNSM 135
           +++ +D V E ELKE GF   R+TKL+CT+GPA  G   LE+LA  GMN+AR+NMCH + 
Sbjct: 93  SSIEVDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTR 152

Query: 136 EWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKVEDGSIWLFTAKKFEG 195
           EWH  +I+++++LNEEKGF +++M+D EGS+IH+ D G  SS K EDG IW FT + F+ 
Sbjct: 153 EWHRMVIERLRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDP 212

Query: 196 SRP-FTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCS 255
             P  TV  +Y+GF+E ++VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +
Sbjct: 213 PLPERTVTVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLT 272

Query: 256 FWRDGRLVM-RNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPVRHLKNYLSTKSA 315
           FWRDG+LV  RN   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ ++HLK+Y+  ++ 
Sbjct: 273 FWRDGKLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARAR 332

Query: 316 KS-IRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELN 375
            S I V+AKIES+++L+NLEEI++ASDG MVARGDLG +IPLEQ+P+ Q++I ++CR+LN
Sbjct: 333 DSDISVIAKIESIDSLKNLEEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLN 392

Query: 376 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 435
           +PVI+ASQLLESM+EYP PTRAEVADVSEAVRQ  DALMLSGESA+G + +KAL+VL+  
Sbjct: 393 RPVIVASQLLESMIEYPIPTRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSV 452

Query: 436 SSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMA 495
           S R+E   RE+ R+  +    +  S  D I+EEICNSAA++AN L VDA+FV T  GHMA
Sbjct: 453 SLRIERMWREQKRHEVIELPSIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMA 512

Query: 496 SLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVK 555
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+++   ++K++G++K
Sbjct: 513 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIK 572

Query: 556 QGDSVLVVSEI 564
            GD ++ VS++
Sbjct: 573 SGDLIIAVSDM 583

BLAST of Cucsat.G16913 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 5.8e-85
Identity = 182/465 (39.14%), Postives = 274/465 (58.92%), Query Frame = 0

Query: 97  RKTKLVCTVGPACSGIADLEKLALEGMNIARLNMCHNSMEWHCDMIKKIKKLNEE-KGFC 156
           RKTK+VCT+GP+ S    + KLA  GMN+ARLNM H     H   I  +K+ N + +   
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 157 ISVMIDIEGSQIHVVDHGAPSSVKVEDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVG 216
           I++M+D +G +  V+    P  + +++G  + F+ K+   +   TV  +Y+ F   +  G
Sbjct: 151 IAIMLDTKGPE--VISGDVPKPILLKEGQEFNFSIKRGVSTED-TVSVNYDDFINDVEAG 210

Query: 217 DSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKD 276
           D +++DGGM +  V  K  + + C   D G    R      R   +  ++   P+++ KD
Sbjct: 211 DILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSR------RHLNVRGKSATLPSITEKD 270

Query: 277 WSDIEFGISEGVDFIALSFVNSADPVRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIV 336
           W DI+FG++  VDF A+SFV  A  V  LK+YL + +A  I V+ KIES +++ NL  I+
Sbjct: 271 WDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYLKSCNA-DIHVIVKIESADSIPNLHSII 330

Query: 337 EASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELNKPVIIASQLLESMVEYPTPTRAE 396
            ASDG MVARGDLG E+P+E++P +QE+I R C+ + KPVI+A+ +LESM+++PTPTRAE
Sbjct: 331 SASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTPTRAE 390

Query: 397 VADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENRNNFLPQHQLG 456
           V+D+S AVR+ ADA+MLSGE+A G Y  KA+ V+ + + R E       ++   P     
Sbjct: 391 VSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTE---SSLQKSTSSPSQSAA 450

Query: 457 VSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRM 516
              H  + E     ++ +AN LS   I V T  G MA +LS NRP   +FA T+++  + 
Sbjct: 451 YKSH--MGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNNERVKQ 510

Query: 517 ALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVKQGDSVLVV 561
            L L  GV P+ ++ S D E   SR I+++ SK LVK G  V +V
Sbjct: 511 RLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLV 539

BLAST of Cucsat.G16913 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 6.0e-82
Identity = 202/591 (34.18%), Postives = 314/591 (53.13%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQV 60
           M   N   +  R   +++     VK LG      GR +V  SRR +   V +    T  +
Sbjct: 14  MLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSV-RSRRVVDTTVRSARVETEVI 73

Query: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGM-------RKTKLVCTVGPACSGIA 120
            +  E+   R E LE  L +    +        +GM       RKTK+VCTVGP+ +   
Sbjct: 74  PVSPEDVPNREEQLERLLEMQQFGDTS------VGMWSKPTVRRKTKIVCTVGPSTNTRE 133

Query: 121 DLEKLALEGMNIARLNMCHNSMEWHCDMIKKIKKLN-EEKGFCISVMIDIEGSQIHVVDH 180
            + KLA  GMN+AR+NM H     H  +I  +K+ N + K   I++M+D +G ++   D 
Sbjct: 134 MIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGD- 193

Query: 181 GAPSSVKVEDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEK 240
             P  + ++ G  + FT ++   S P  V  +Y+ F   +  GD +++DGGM +F V  K
Sbjct: 194 -LPQPIMLDPGQEFTFTIER-GVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSK 253

Query: 241 IGNDLSCRCTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIAL 300
             + + C   D G    R      R   +  ++   P+++ KDW DI+FG+   VDF A+
Sbjct: 254 TKDSVKCEVVDGGELKSR------RHLNVRGKSATLPSITEKDWEDIKFGVENKVDFYAV 313

Query: 301 SFVNSADPVRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEI 360
           SFV  A  V  LK YL    A  I V+ KIES +++ NL  I+ ASDG MVARGDLG E+
Sbjct: 314 SFVKDAQVVHELKKYLQNSGA-DIHVIVKIESADSIPNLHSIITASDGAMVARGDLGAEL 373

Query: 361 PLEQIPAVQEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALML 420
           P+E++P +QEEI  +CR + K VI+A+ +LESM+ +PTPTRAEV+D++ AVR+ ADA+ML
Sbjct: 374 PIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAVML 433

Query: 421 SGESAIGSYGQKALSVLQMASSRME--LWSREENRNNFLPQHQLGVSLHDRIAEEICNSA 480
           SGE+A G +  KA  V+   + R E  + S E   N       LG +  + ++E     A
Sbjct: 434 SGETAHGKFPLKAAGVMHTVALRTEATITSGEMPPN-------LGQAFKNHMSEMFAYHA 493

Query: 481 AELANRLSVDAIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDL 540
             ++N L    + V T  G MA LLS  RP   I+A T++   +  L L  GV P+ ++ 
Sbjct: 494 TMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEF 553

Query: 541 SEDIEANISRGIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 582
           ++D E   +  +  +  +G+VK+G+ + +V   +     S ++ +IQ++ +
Sbjct: 554 TDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of Cucsat.G16913 vs. NCBI nr
Match: XP_004150267.1 (plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN52735.1 hypothetical protein Csa_015140 [Cucumis sativus])

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 581/582 (99.83%), Postives = 581/582 (99.83%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRV NGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVVNGVKATMQV 60

Query: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120
           DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL
Sbjct: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120

Query: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582
           GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582

BLAST of Cucsat.G16913 vs. NCBI nr
Match: KAA0044313.1 (plastidial pyruvate kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1111 bits (2874), Expect = 0.0
Identity = 567/582 (97.42%), Postives = 575/582 (98.80%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMG RTVWC+RRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120
           DL+DEERL+RLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCT+GPACSGI DLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS EWHCDMIKKIKKLNEEKGFCISVMIDIEGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTA+KFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLV+RNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYL TKSAKSIRVLAKIESLEALQNLEEI+EASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN  NFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582
           GIEVVKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Cucsat.G16913 vs. NCBI nr
Match: XP_008454424.1 (PREDICTED: plastidial pyruvate kinase 1, chloroplastic-like [Cucumis melo] >ADN33819.1 pyruvate kinase [Cucumis melo subsp. melo] >TYK29442.1 plastidial pyruvate kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1109 bits (2869), Expect = 0.0
Identity = 566/582 (97.25%), Postives = 574/582 (98.63%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMG RTVWC+RRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120
           DL+DEERL+RLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCT+GPACSGI DLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS EWHCDMIKKIKKLNEEKGFCISVMIDIEGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTA+KFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLV+RNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYL TKSAKSIRVLAKIESLEALQNLEEI+EASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN  NFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582
           GIEVVKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Cucsat.G16913 vs. NCBI nr
Match: XP_038905899.1 (plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1053 bits (2723), Expect = 0.0
Identity = 538/582 (92.44%), Postives = 563/582 (96.74%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQV 60
           MAEVNA+MIR+R +F+ ++PVLDVKR GF MPV+GR TVWC+RRR +G VGNGVKATMQV
Sbjct: 1   MAEVNAIMIRSRFDFIAKSPVLDVKRSGFGMPVIGR-TVWCNRRRTRGGVGNGVKATMQV 60

Query: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120
           DLEDEERLKRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCT+GPAC+ I DLEKLAL
Sbjct: 61  DLEDEERLKRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACNAIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS EWHCD+I+KIKKLNE+KGFCISVMID EGSQIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEKKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTA+KFEGSRPFTVQASYEGFSEGI+VGDSIVIDGGMATFEVIEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLV+RNHESPTLSSKDWSDI+FGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIKFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYL TKSAKS+RVLAKIESLE+LQNLEEI+EASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VQHLKNYLLTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRME WSREENR NFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMESWSREENRKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRP P IFALTDDDSTRMALNLQWGV PLRID SEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPSPLIFALTDDDSTRMALNLQWGVVPLRIDFSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582
           GIE+VKSKGLVK+GDSVLVVSEISPARAASMASQSIQLKTIV
Sbjct: 541 GIELVKSKGLVKKGDSVLVVSEISPARAASMASQSIQLKTIV 581

BLAST of Cucsat.G16913 vs. NCBI nr
Match: XP_023553283.1 (plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1050 bits (2716), Expect = 0.0
Identity = 535/581 (92.08%), Postives = 558/581 (96.04%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRA CNF+ +NPVLDVKR GF MPV GR TVWC+RRR  GRVGNGVKA MQV
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGR-TVWCNRRRTTGRVGNGVKAVMQV 60

Query: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120
           DLED ERL+RLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCT+GPACS I DLEKLAL
Sbjct: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS EWHCD+I+KIKKLNE KGFCISVMID EGSQIHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180

Query: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTA+KFEGSRPFTVQASYEGFSEGI+VGDS+VIDGGMATFEVIEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGR+V+RNHESPTLSSKDWSDIEFGI+EGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYLSTKSA+S+RVLAKIESLE+LQNLEEI+EASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VKHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMAS RMELWSREENR NFLP +QLGVSL DRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 581
           GIE+VKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI
Sbjct: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 580

BLAST of Cucsat.G16913 vs. ExPASy TrEMBL
Match: A0A0A0KSY4 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000560 PE=3 SV=1)

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 581/582 (99.83%), Postives = 581/582 (99.83%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRV NGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVVNGVKATMQV 60

Query: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120
           DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL
Sbjct: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120

Query: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582
           GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582

BLAST of Cucsat.G16913 vs. ExPASy TrEMBL
Match: A0A5A7TLC2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350 PE=3 SV=1)

HSP 1 Score: 1111 bits (2874), Expect = 0.0
Identity = 567/582 (97.42%), Postives = 575/582 (98.80%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMG RTVWC+RRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120
           DL+DEERL+RLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCT+GPACSGI DLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS EWHCDMIKKIKKLNEEKGFCISVMIDIEGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTA+KFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLV+RNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYL TKSAKSIRVLAKIESLEALQNLEEI+EASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN  NFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582
           GIEVVKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Cucsat.G16913 vs. ExPASy TrEMBL
Match: A0A5D3E0J9 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00350 PE=3 SV=1)

HSP 1 Score: 1109 bits (2869), Expect = 0.0
Identity = 566/582 (97.25%), Postives = 574/582 (98.63%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMG RTVWC+RRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120
           DL+DEERL+RLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCT+GPACSGI DLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS EWHCDMIKKIKKLNEEKGFCISVMIDIEGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTA+KFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLV+RNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYL TKSAKSIRVLAKIESLEALQNLEEI+EASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN  NFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582
           GIEVVKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Cucsat.G16913 vs. ExPASy TrEMBL
Match: E5GBH7 (Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1109 bits (2869), Expect = 0.0
Identity = 566/582 (97.25%), Postives = 574/582 (98.63%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMG RTVWC+RRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120
           DL+DEERL+RLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCT+GPACSGI DLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS EWHCDMIKKIKKLNEEKGFCISVMIDIEGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTA+KFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLV+RNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYL TKSAKSIRVLAKIESLEALQNLEEI+EASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN  NFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582
           GIEVVKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Cucsat.G16913 vs. ExPASy TrEMBL
Match: A0A1S3BYK3 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103494833 PE=3 SV=1)

HSP 1 Score: 1109 bits (2869), Expect = 0.0
Identity = 566/582 (97.25%), Postives = 574/582 (98.63%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMG RTVWC+RRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120
           DL+DEERL+RLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCT+GPACSGI DLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS EWHCDMIKKIKKLNEEKGFCISVMIDIEGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTA+KFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLV+RNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYL TKSAKSIRVLAKIESLEALQNLEEI+EASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN  NFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582
           GIEVVKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIK04.5e-17059.75Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q431171.7e-16958.45Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Q405451.6e-16457.03Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q405465.8e-8539.14Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9FLW96.0e-8234.18Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004150267.10.099.83plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN52735.1 hypoth... [more]
KAA0044313.10.097.42plastidial pyruvate kinase 1 [Cucumis melo var. makuwa][more]
XP_008454424.10.097.25PREDICTED: plastidial pyruvate kinase 1, chloroplastic-like [Cucumis melo] >ADN3... [more]
XP_038905899.10.092.44plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida][more]
XP_023553283.10.092.08plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KSY40.099.83Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000560 PE=3 SV=1[more]
A0A5A7TLC20.097.42Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350... [more]
A0A5D3E0J90.097.25Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G0035... [more]
E5GBH70.097.25Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3BYK30.097.25Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103494833 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 111..127
score: 35.11
coord: 67..91
score: 57.66
coord: 42..66
score: 49.11
coord: 15..41
score: 52.31
coord: 92..110
score: 54.25
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 4..278
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 130..275
e-value: 2.3E-30
score: 107.1
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 157..260
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 11..132
e-value: 1.3E-44
score: 152.6
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 5..129
e-value: 1.1E-52
score: 180.8
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 161..261
e-value: 2.2E-22
score: 79.5
NoneNo IPR availablePANTHERPTHR11817:SF21PYRUVATE KINASEcoord: 4..278
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 13..25
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 9..134

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G16913.T1Cucsat.G16913.T1mRNA
Cucsat.G16913.T2Cucsat.G16913.T2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity