Cucsat.G1683 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G1683
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Protein-lysine N-methyltransferase
Location	ctg1002: 47653 .. 51646 (-)
RNA-Seq Expression	Cucsat.G1683
Synteny	Cucsat.G1683

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTAATATGTATATATAAATAAAAGAAAAGTATACTTTACCTAAAAAGCATTCCCAACAAATAGAATTAATTCCCTCTATCATTTATCGCCGCCGCTTCTGTTCTTCGCGCGAGAGAAAACGGCCTCAAAGATTACATCAGAAATGGAGTCACCCACCAAATCGGAGAGCATTTGTAGGGACCCTACAGTTGTTGAAGAGGATGATGTTGATGATGATCCGCCATTGCTGAGCTCTCATGCCCTAGCTGCACTAAAGGAGTTCCTTGCCGAACAAACCCAGCCCTCCACTGAGCCTCACTTGGAAAATGGAGTTTCCGAGGATTCGGAGGTCGCTTTAGTGGCGGAGGATTGGAGGCTGAGTCAATTCTGGTACGACCGGGTCACGGCGGAGACAGTGGCTAATGAGGTTCTTACGCTATGCCAGAACTTTGATTCTCCTCGAGTCGCATGTATTGCTTGCCCGACTCTCTACACTTATCTGAAGGTAATTGGATAGTCGTTTTTGTTTATCATATTTTGGAACTTGAATTTACTTGGTTGAGACTGGAGTTTCAAGTGGGGAGCAGATAAATGTACATCCCTTGAATCATACCGATGATAAGAATGTGTTTTACTCGCTTTATACCAACGTAACATGTTATGAACTTATGGTACACCTCACACTTTGATTTTAAAACAAATATAAGTTATTTTAAAACAAATATAAGTTGCACAAGGAATGATTTGTTGTTTCACTTTATAGTTAAAAACTGGTTTTTGATGAGAGGAAAATTTCGTTCGTGGAAGAAGGGACGAGGCTGGATATGTTAAGAGGCTGATGGACTCTCTAATTAATTGGATATTGAAAGGGAAAATTTAGAAATTGACATATCTAAGAGGAAAAAAAAAGCCTGTTTCCAAGCTCAGGATCACTAAATAGCCCTGAAATATCTGATGTATGAGCTCCAAAGCCATGGCTCCAACGAACATGGTCCACTCGGCTTTAGGTTTGCCATGAAGACAGTGTAAAAAACACTTCACAATTGCAGTAGAAAATAGTTCAACTGATGGCCAGCACCATGTAGATTATGGAAGAGAACCGGGACCAAACTAATTGGAAAAGGTGCATTTTATAGGAAGGCTTCCATTTATTTTCATTAACTTACTACAGGCGAGAGTCCAAAGAAGAATTTTATGTTTATTTTTATTTAGGGGCCTTGCTAGACATTTTCGCTTGGAAAAGCTTAGTGATTCACATTGCCCTTAGCATTCGTTAGATCCTTGGACCAGTGATATTTCACCTTCTGTTTACTTCTTGGCCATTCGGTTTGATGTCTACACTGGTACACCAAAGGTTACCCCATTCTACAACTTCCCTATCATTAAGAGTCCTTTTGAATCATTGGATTTCTGAGCCAATGATCAGCATCATAAGTCATAACTCCAAGCCCTTTCCACAAGAGTTAATGACAAACTTGTTCAGTAGTCCTTCAAATTTTGAAGGGATTTACTACAAGGAGAGATAGTATATGGGTGTATTTGCTCAGTGACTTGAAGCTTGAATTGATGTATGTTTATCATCTTTAGAAAATTAGGACTTCATTTGTTACTGTTTCCCAATTCACATTGTCTCAGTCCATTCATAGTGATTAGAAGCCTTGTTCTTTTCTGATCTCTTTCATTCACCCCTTCAGGATTTGAGACTATTAATACTTCCTGGAAGGATTTGAGCTTTTTTCTATTGGCCTCCTAAAGTACTAATTATTTTAAGCTATTTTTGTAGACATTAACCCATGATATTTTAGACTATAAGATTTGACAAATTAATAGTCTCTATAGTCTCAAACCCTCCAAAGAAACAGGAAGCTCAACAATATAGAGACTATTATTTGTCAAGGCTTATAGCCTCAAAACCTTATGGGTTAATTTTGTCGAAACATTACTTGTTGAAATATGGTTTGAAAGGAATCAAAGGGTTTTCCATGATAAACCCCTACAGTGGATTGACTGTTTTGAAGTTGCAAGACTCAATGATTCTACTTGGTGTTCTCTTTCTAAGCTTTTTGCGAGATTTTCAATTATTTATTTGAACTTAGGAGCTTTTATCTTGTCTATTTTGATCATTGAATTGTCTTGTACCTTTAATGGCTTATATTTTGTTTCCTTGTAATTTGAAATATAATTCTCCTTTCATTTTATCAATGATAAGATGTTTTCCTAAAAAAAGAAAAAAAGAAGTTAGGATTAGAAATATGTAGTTTGAACAGAAAATGAAATCTGACCATGAATTCATTGTACTGCAAATTCACAAGGTTATTTATTATTTATTTTTTTCAAGTTGATTCATAAACATTACATTTTAAATTTAAATTATAAATATTCTCTAACACAATTTGATTTGGTTATGATAATAGTTAATGTTTGGTTGCGAGGTTTTGCACTGGTGCAGTGCACCAGTGCCTATAATTGAGCCTAAGACATTAAATAGTCTATATGAGTTGTGAACATTCTTTTGAAACGAAAACCTTATCCTTTTTTCCAAACCAGATTGTTATTTTCTTCACCTTTTTGTTTGTTATAAACCAAAAGTAACTCGTTCTATTTTGAAGCCATTTTCTTCCAGAACTAACCAGTTTCTCAATTGACTAATATATTGCCTTAGTCTGATCTTCATTTGTCAGAAAATTGGCCCCAACGTATCTGCACAACTTCTTGAGTATGACAAACGCTTTTCCCAACATGGTAGTGAATTCACATTTTACGATTACAATGAACCGGAAGAATTGCCAATGGAATTGAAGCACAATTTTCAGATTATAGTAGCGGATCCTCCTTACCTGGTGAGAGATAATGTCTTTGCCTATGTTTAGTTTTTCTTTTCTTTTTTGTACACTTTTGGATGCTTAATTTGTGAATCAGGGTTAAATATCCTTCTCGTTTACTGGATTTTTGTGTATGTGTGCGCACATGTGATTATTATCACATCCTTCTCAGAATGCTTTACGGAAAAATCCGTTTCTTTACTCTCCCAAGTTTGACTGTTCTATTTGAACATTCTAGTTGTGCGTAACACTAACAATACATGAGTTTACGTCATATAAAGACTGAACTCATACAAACGACAGTTTTTCTACAAGACCGTATAACGCAGCTTTCCCAGTTGATGATTCTGTATTCTGATTGTATCAGTGCTTTGGATTATATATATATATATATATATTTGTTAAACAATGCTTCTGTACCAAGCTATGATTAATTTGTGCTCCGTTTATGTTCCTTCTGGCAGAGCAAAGAATGTTTGGAGAAAGTCACTCATACAATTTCTTTTCTAGCACAACACAGTGAATCTTACCTGCTATTACTAACAGGTATTGCAACGATGAACAACCCTTGTGCCGCTATTATATATTTCACGTTGCAATTTGTTTATATATCTGAGATAATTGTGATTTTATTCTATTGGTTTCCTTATGATTCCACATTGCATCATCAAATTGCAGGAGAAGTGCAAGCAGAAAGAGCAGCTGAGCTAATGGGTTTCCATACGTGTGGTTTTAGACCACACCACTCTAGCAAGCTCGGGAACGAGTTCCGATTGTTCACGAACTATAATCCGGGGACAAGATTAGGAGGTTGGGAGCAATAGATTTTGGATCACTATTTTTCTCTCTTGTAATTTTAAAGAGGCACGTGGATTAGAAATCTCTCATCTCATCATTTTGTATTCCGTTCAAGTGGTGGTGATGGTTCATGCCTACAGTCGAAGAAGGATTCAGTGAGAACTCTGAACTTGTAGAAGTGAATTGGTGCAAAAGCCAAAATGTAATGGGCAAAGTGCTTACCGTTGGGAGTGTAATTAATTTAAAGTACCGAGGGAAAACTAACGCTCACTAGTACTTGTAGTGGAAATATTTGTAAGCTCCATGATTTCTATTAGTTGGGGAATAGGCTGTTCATTTTTGTTGTTGAGGGCTGATTTTGAAATGACTAAAAACCCTTTTGTTGTTTTTAAAATCATTTAAATCATTCAAAATTAATTTTAATAGTATGAAAAAAGCATTTAAAGGTGTAAAATTAAAGATTGAATTAATTTTGATTATGTCACAATGTGGATAAAGAATGGAATTCTGAAAGCAGTTCAAAGGAGTCCATTTTGCTAGGCTTGCGAACATTAAATCAACTCGGCTATTGAAATTTA

Coding sequence (CDS)

ATGGAGTCACCCACCAAATCGGAGAGCATTTGTAGGGACCCTACAGTTGTTGAAGAGGATGATGTTGATGATGATCCGCCATTGCTGAGCTCTCATGCCCTAGCTGCACTAAAGGAGTTCCTTGCCGAACAAACCCAGCCCTCCACTGAGCCTCACTTGGAAAATGGAGTTTCCGAGGATTCGGAGGTCGCTTTAGTGGCGGAGGATTGGAGGCTGAGTCAATTCTGGTACGACCGGGTCACGGCGGAGACAGTGGCTAATGAGGTTCTTACGCTATGCCAGAACTTTGATTCTCCTCGAGTCGCATGTATTGCTTGCCCGACTCTCTACACTTATCTGAAGAAAATTGGCCCCAACGTATCTGCACAACTTCTTGAGTATGACAAACGCTTTTCCCAACATGGTAGTGAATTCACATTTTACGATTACAATGAACCGGAAGAATTGCCAATGGAATTGAAGCACAATTTTCAGATTATAGTAGCGGATCCTCCTTACCTGAGCAAAGAATGTTTGGAGAAAGTCACTCATACAATTTCTTTTCTAGCACAACACAGTGAATCTTACCTGCTATTACTAACAGGAGAAGTGCAAGCAGAAAGAGCAGCTGAGCTAATGGGTTTCCATACGTGTGGTTTTAGACCACACCACTCTAGCAAGCTCGGGAACGAGTTCCGATTGTTCACGAACTATAATCCGGGGACAAGATTAGGAGGTTGGGAGCAATAG

Protein sequence

MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSEDSEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLKKIGPNVSAQLLEYDKRFSQHGSEFTFYDYNEPEELPMELKHNFQIIVADPPYLSKECLEKVTHTISFLAQHSESYLLLLTGEVQAERAAELMGFHTCGFRPHHSSKLGNEFRLFTNYNPGTRLGGWEQ

Homology

[Close]

BLAST of Cucsat.G1683 vs. ExPASy Swiss-Prot
Match: Q6GN98 (EEF1A lysine methyltransferase 1 OS=Xenopus laevis OX=8355 GN=eef1akmt1 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.7e-13
Identity = 45/98 (45.92%), Postives = 59/98 (60.20%), Query Frame = 0


Query: 17  VEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSEDSEVALVAEDWRLSQFW 76
           +E  D DD  P LSSHALAAL+EF AEQ Q   E        E   V  V EDW+LSQFW
Sbjct: 1   MEGSDDDDGVPQLSSHALAALQEFYAEQQQ--REAQKLGPDHEKFSVGSVEEDWQLSQFW 60

Query: 77  YDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 115
           Y   TA ++A E + +C   ++ R+ACI+ P++Y  L+
Sbjct: 61  YSDETALSLAKEAIEVCG--ENGRIACISAPSIYQKLR 94

[Close]

BLAST of Cucsat.G1683 vs. ExPASy Swiss-Prot
Match: Q8WVE0 (EEF1A lysine methyltransferase 1 OS=Homo sapiens OX=9606 GN=EEF1AKMT1 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.5e-12
Identity = 37/92 (40.22%), Postives = 57/92 (61.96%), Query Frame = 0


Query: 23  DDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSEDSEVALVAEDWRLSQFWYDRVTA 82
           DD+ P LS+HALAAL+EF AEQ Q      +E G  +   + ++ E+W+LSQFWY + TA
Sbjct: 6   DDETPQLSAHALAALQEFYAEQKQ-----QIEPGEDDKYNIGIIEENWQLSQFWYSQETA 65

Query: 83  ETVANEVLTLCQNFDSPRVACIACPTLYTYLK 115
             +A E +      +  R+AC++ P++Y  L+
Sbjct: 66  LQLAQEAIAAVG--EGGRIACVSAPSVYQKLR 90

[Close]

BLAST of Cucsat.G1683 vs. ExPASy Swiss-Prot
Match: Q5ZKT6 (EEF1A lysine methyltransferase 1 OS=Gallus gallus OX=9031 GN=EEF1AKMT1 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 5.6e-12
Identity = 42/95 (44.21%), Postives = 55/95 (57.89%), Query Frame = 0


Query: 20  DDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSEDSEVALVAEDWRLSQFWYDR 79
           DD DDD P LSSH LAAL+EF  EQ Q         G ++ S V  + EDW+LSQFWY  
Sbjct: 2   DDDDDDIPQLSSHTLAALQEFYLEQ-QQREGMKTSQGFNQYS-VGSIEEDWQLSQFWYSD 61

Query: 80  VTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 115
            TA  +ANE +         R+AC++ P++Y  L+
Sbjct: 62  ETASCLANEAIVAAGK--GGRIACVSAPSVYQKLR 92

[Close]

BLAST of Cucsat.G1683 vs. ExPASy Swiss-Prot
Match: Q9CY45 (EEF1A lysine methyltransferase 1 OS=Mus musculus OX=10090 GN=Eef1akmt1 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 4.7e-11
Identity = 39/92 (42.39%), Postives = 56/92 (60.87%), Query Frame = 0


Query: 23  DDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSEDSEVALVAEDWRLSQFWYDRVTA 82
           DDD P LSSH LAAL+EF AEQ Q S  P  +    +   V ++ E+W+LSQFWY + TA
Sbjct: 6   DDDIPQLSSHTLAALQEFYAEQKQ-SVNPRGD----DKYNVGVIEENWQLSQFWYSQDTA 65

Query: 83  ETVANEVLTLCQNFDSPRVACIACPTLYTYLK 115
             +A E +      +  R+AC++ P++Y  L+
Sbjct: 66  LRLAREAIDAAG--EGGRIACVSAPSVYQKLR 90

[Close]

BLAST of Cucsat.G1683 vs. ExPASy Swiss-Prot
Match: Q17QF2 (EEF1A lysine methyltransferase 1 OS=Bos taurus OX=9913 GN=EEF1AKMT1 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 8.9e-10
Identity = 35/96 (36.46%), Postives = 57/96 (59.38%), Query Frame = 0


Query: 20  DDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSEDS-EVALVAEDWRLSQFWYD 79
           D  ++ PP LSS+ALAAL+EF AEQ     + H  +   +D   + ++ E+W+LSQFWY 
Sbjct: 3   DSENEGPPQLSSYALAALQEFYAEQ-----QHHHSDLCGDDKYNIGIIEENWQLSQFWYS 62

Query: 80  RVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 115
             TA  +A + +      +  R+AC++ P++Y  L+
Sbjct: 63  PETATCLAEDAVAAAG--EGGRIACVSAPSVYQKLR 91

[Close]

BLAST of Cucsat.G1683 vs. NCBI nr
Match: XP_004143344.1 (EEF1A lysine methyltransferase 1 [Cucumis sativus] >KGN48324.1 hypothetical protein Csa_003182 [Cucumis sativus])

HSP 1 Score: 227 bits (579), Expect = 8.00e-73
Identity = 113/114 (99.12%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60
           MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED
Sbjct: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 114
           SEVALVAEDWRLSQFWYDRVTAETVA+EVLTLCQNFDSPRVACIACPTLYTYLK
Sbjct: 61  SEVALVAEDWRLSQFWYDRVTAETVADEVLTLCQNFDSPRVACIACPTLYTYLK 114

[Close]

BLAST of Cucsat.G1683 vs. NCBI nr
Match: XP_008462634.1 (PREDICTED: protein-lysine N-methyltransferase N6AMT2 [Cucumis melo] >KAA0025209.1 protein-lysine N-methyltransferase N6AMT2 [Cucumis melo var. makuwa] >TYK07457.1 protein-lysine N-methyltransferase N6AMT2 [Cucumis melo var. makuwa])

HSP 1 Score: 214 bits (546), Expect = 8.25e-68
Identity = 107/114 (93.86%), Postives = 109/114 (95.61%), Query Frame = 0


Query: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60
           MESPTKSES+CRDP VV EDDVDDDPPLLS HALAALKEFLAEQTQPSTEPHLENGVSED
Sbjct: 1   MESPTKSESVCRDPRVVGEDDVDDDPPLLSFHALAALKEFLAEQTQPSTEPHLENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 114
           SEVALVAEDWRLSQFWYDR TAETVA+EVLTLCQ FDSPRVACIACPTLYTYLK
Sbjct: 61  SEVALVAEDWRLSQFWYDRHTAETVADEVLTLCQKFDSPRVACIACPTLYTYLK 114

[Close]

BLAST of Cucsat.G1683 vs. NCBI nr
Match: XP_038880976.1 (EEF1A lysine methyltransferase 1 [Benincasa hispida])

HSP 1 Score: 208 bits (530), Expect = 2.15e-65
Identity = 105/114 (92.11%), Postives = 109/114 (95.61%), Query Frame = 0


Query: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60
           MESP+KSESICRDP +VE+DD D DPPLLSSHALAALKEFL EQTQPSTEPHLENGVSED
Sbjct: 1   MESPSKSESICRDPRIVEDDD-DADPPLLSSHALAALKEFLTEQTQPSTEPHLENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 114
           SEVALVAEDWRLSQFWYDR TAETVANEVLTLCQNF+SPRVACIACPTLYTYLK
Sbjct: 61  SEVALVAEDWRLSQFWYDRDTAETVANEVLTLCQNFNSPRVACIACPTLYTYLK 113

[Close]

BLAST of Cucsat.G1683 vs. NCBI nr
Match: KAG6604311.1 (EEF1A lysine methyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034465.1 EEF1A lysine methyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 187 bits (476), Expect = 3.34e-57
Identity = 95/114 (83.33%), Postives = 101/114 (88.60%), Query Frame = 0


Query: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60
           MESP+KSES CRDP + EE+D DDDPPLLSSHALAALKEFL EQ Q   + HLE+GVSED
Sbjct: 1   MESPSKSESTCRDPRIEEEND-DDDPPLLSSHALAALKEFLDEQAQTQPDVHLESGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 114
           SEVALV EDWRLSQFWYDR TAETV NEVLTLCQNF+SPRVACIACPTLYTYLK
Sbjct: 61  SEVALVTEDWRLSQFWYDRDTAETVTNEVLTLCQNFESPRVACIACPTLYTYLK 113

[Close]

BLAST of Cucsat.G1683 vs. NCBI nr
Match: ALO20340.1 (N(6)-adenine-specific DNA methyltransferase 2 [Momordica charantia])

HSP 1 Score: 186 bits (473), Expect = 8.95e-57
Identity = 95/114 (83.33%), Postives = 102/114 (89.47%), Query Frame = 0


Query: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60
           MESPTKS +IC D  + EE+D   DPPLLSSHALAALKEFLAEQ+QPS+E H ENGVSED
Sbjct: 1   MESPTKSPTICTDLRIEEEED---DPPLLSSHALAALKEFLAEQSQPSSELHSENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 114
           S+VALVAEDWRLSQFWYDR TAETVANEVLTLCQ FDSPRVAC+ACPTLYTYLK
Sbjct: 61  SQVALVAEDWRLSQFWYDRDTAETVANEVLTLCQKFDSPRVACVACPTLYTYLK 111

[Close]

BLAST of Cucsat.G1683 vs. ExPASy TrEMBL
Match: A0A0A0KHM5 (Protein-lysine N-methyltransferase Csa_6G476640 OS=Cucumis sativus OX=3659 GN=Csa_6G476640 PE=3 SV=1)

HSP 1 Score: 227 bits (579), Expect = 3.88e-73
Identity = 113/114 (99.12%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60
           MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED
Sbjct: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 114
           SEVALVAEDWRLSQFWYDRVTAETVA+EVLTLCQNFDSPRVACIACPTLYTYLK
Sbjct: 61  SEVALVAEDWRLSQFWYDRVTAETVADEVLTLCQNFDSPRVACIACPTLYTYLK 114

[Close]

BLAST of Cucsat.G1683 vs. ExPASy TrEMBL
Match: A0A5D3C6D8 (Protein-lysine N-methyltransferase E5676_scaffold202G001880 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001880 PE=3 SV=1)

HSP 1 Score: 214 bits (546), Expect = 3.99e-68
Identity = 107/114 (93.86%), Postives = 109/114 (95.61%), Query Frame = 0


Query: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60
           MESPTKSES+CRDP VV EDDVDDDPPLLS HALAALKEFLAEQTQPSTEPHLENGVSED
Sbjct: 1   MESPTKSESVCRDPRVVGEDDVDDDPPLLSFHALAALKEFLAEQTQPSTEPHLENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 114
           SEVALVAEDWRLSQFWYDR TAETVA+EVLTLCQ FDSPRVACIACPTLYTYLK
Sbjct: 61  SEVALVAEDWRLSQFWYDRHTAETVADEVLTLCQKFDSPRVACIACPTLYTYLK 114

[Close]

BLAST of Cucsat.G1683 vs. ExPASy TrEMBL
Match: A0A1S3CHE6 (Protein-lysine N-methyltransferase LOC103500945 OS=Cucumis melo OX=3656 GN=LOC103500945 PE=3 SV=1)

HSP 1 Score: 214 bits (546), Expect = 3.99e-68
Identity = 107/114 (93.86%), Postives = 109/114 (95.61%), Query Frame = 0


Query: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60
           MESPTKSES+CRDP VV EDDVDDDPPLLS HALAALKEFLAEQTQPSTEPHLENGVSED
Sbjct: 1   MESPTKSESVCRDPRVVGEDDVDDDPPLLSFHALAALKEFLAEQTQPSTEPHLENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 114
           SEVALVAEDWRLSQFWYDR TAETVA+EVLTLCQ FDSPRVACIACPTLYTYLK
Sbjct: 61  SEVALVAEDWRLSQFWYDRHTAETVADEVLTLCQKFDSPRVACIACPTLYTYLK 114

[Close]

BLAST of Cucsat.G1683 vs. ExPASy TrEMBL
Match: A0A6J1BT81 (Protein-lysine N-methyltransferase LOC111005552 OS=Momordica charantia OX=3673 GN=LOC111005552 PE=3 SV=1)

HSP 1 Score: 186 bits (473), Expect = 4.34e-57
Identity = 95/114 (83.33%), Postives = 102/114 (89.47%), Query Frame = 0


Query: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60
           MESPTKS +IC D  + EE+D   DPPLLSSHALAALKEFLAEQ+QPS+E H ENGVSED
Sbjct: 1   MESPTKSPTICTDLRIEEEED---DPPLLSSHALAALKEFLAEQSQPSSELHSENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 114
           S+VALVAEDWRLSQFWYDR TAETVANEVLTLCQ FDSPRVAC+ACPTLYTYLK
Sbjct: 61  SQVALVAEDWRLSQFWYDRDTAETVANEVLTLCQKFDSPRVACVACPTLYTYLK 111

[Close]

BLAST of Cucsat.G1683 vs. ExPASy TrEMBL
Match: A0A0S2I9N1 (Protein-lysine N-methyltransferase OS=Momordica charantia OX=3673 PE=2 SV=1)

HSP 1 Score: 186 bits (473), Expect = 4.34e-57
Identity = 95/114 (83.33%), Postives = 102/114 (89.47%), Query Frame = 0


Query: 1   MESPTKSESICRDPTVVEEDDVDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSED 60
           MESPTKS +IC D  + EE+D   DPPLLSSHALAALKEFLAEQ+QPS+E H ENGVSED
Sbjct: 1   MESPTKSPTICTDLRIEEEED---DPPLLSSHALAALKEFLAEQSQPSSELHSENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 114
           S+VALVAEDWRLSQFWYDR TAETVANEVLTLCQ FDSPRVAC+ACPTLYTYLK
Sbjct: 61  SQVALVAEDWRLSQFWYDRDTAETVANEVLTLCQKFDSPRVACVACPTLYTYLK 111

[Close]

BLAST of Cucsat.G1683 vs. TAIR 10
Match: AT3G58470.1 (nucleic acid binding;methyltransferases )

HSP 1 Score: 109.8 bits (273), Expect = 1.7e-24
Identity = 65/118 (55.08%), Postives = 77/118 (65.25%), Query Frame = 0


Query: 1   MESPTKSESICRDPTVVEEDDVDDDPPL-LSSHALAALKEFLAEQ--TQPSTEPHLE-NG 60
           ME   + + +    +   + DV+DD PL LSS ALAAL+EFLA+Q  T  ST P     G
Sbjct: 1   MEKEDELQKLNGQVSAPRDGDVEDDDPLVLSSQALAALREFLADQNKTVASTPPASSVAG 60

Query: 61  VSEDSEVALVAEDWRLSQFWYDRVTAETVANEVLTLCQNFDSPRVACIACPTLYTYLK 115
             E  +V LV EDWRLSQFWY+  TAETVA+EV+TL Q     RVACIACPTLY YLK
Sbjct: 61  GEESDKVELVTEDWRLSQFWYEPETAETVADEVVTLSQRIPGCRVACIACPTLYVYLK 118

The following BLAST results are available for this feature:

BLAST of Cucsat.G1683 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6GN98	1.7e-13	45.92	EEF1A lysine methyltransferase 1 OS=Xenopus laevis OX=8355 GN=eef1akmt1 PE=2 SV=...	[more]
Q8WVE0	1.5e-12	40.22	EEF1A lysine methyltransferase 1 OS=Homo sapiens OX=9606 GN=EEF1AKMT1 PE=1 SV=1	[more]
Q5ZKT6	5.6e-12	44.21	EEF1A lysine methyltransferase 1 OS=Gallus gallus OX=9031 GN=EEF1AKMT1 PE=2 SV=1	[more]
Q9CY45	4.7e-11	42.39	EEF1A lysine methyltransferase 1 OS=Mus musculus OX=10090 GN=Eef1akmt1 PE=1 SV=1	[more]
Q17QF2	8.9e-10	36.46	EEF1A lysine methyltransferase 1 OS=Bos taurus OX=9913 GN=EEF1AKMT1 PE=2 SV=1	[more]

BLAST of Cucsat.G1683 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143344.1	8.00e-73	99.12	EEF1A lysine methyltransferase 1 [Cucumis sativus] >KGN48324.1 hypothetical prot...	[more]
XP_008462634.1	8.25e-68	93.86	PREDICTED: protein-lysine N-methyltransferase N6AMT2 [Cucumis melo] >KAA0025209....	[more]
XP_038880976.1	2.15e-65	92.11	EEF1A lysine methyltransferase 1 [Benincasa hispida]	[more]
KAG6604311.1	3.34e-57	83.33	EEF1A lysine methyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia...	[more]
ALO20340.1	8.95e-57	83.33	N(6)-adenine-specific DNA methyltransferase 2 [Momordica charantia]	[more]

BLAST of Cucsat.G1683 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KHM5	3.88e-73	99.12	Protein-lysine N-methyltransferase Csa_6G476640 OS=Cucumis sativus OX=3659 GN=Cs...	[more]
A0A5D3C6D8	3.99e-68	93.86	Protein-lysine N-methyltransferase E5676_scaffold202G001880 OS=Cucumis melo var....	[more]
A0A1S3CHE6	3.99e-68	93.86	Protein-lysine N-methyltransferase LOC103500945 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1BT81	4.34e-57	83.33	Protein-lysine N-methyltransferase LOC111005552 OS=Momordica charantia OX=3673 G...	[more]
A0A0S2I9N1	4.34e-57	83.33	Protein-lysine N-methyltransferase OS=Momordica charantia OX=3673 PE=2 SV=1	[more]

BLAST of Cucsat.G1683 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58470.1	1.7e-24	55.08	nucleic acid binding;methyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 72..216 e-value: 1.1E-5 score: 26.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..28
None	No IPR available	PANTHER	PTHR13200:SF0	EEF1A LYSINE METHYLTRANSFERASE 1	coord: 16..241
IPR041370	Methyltransferase EEF1AKMT1/ZCCHC4	PFAM	PF10237	N6-adenineMlase	coord: 68..232 e-value: 7.3E-51 score: 172.3
IPR019369	Protein-lysine N-methyltransferase Efm5/EEF1AKMT1	PANTHER	PTHR13200	UNCHARACTERIZED	coord: 16..241
IPR019369	Protein-lysine N-methyltransferase Efm5/EEF1AKMT1	HAMAP	MF_03187	Methyltr_EFM5	coord: 21..237 score: 35.833881
IPR002052	DNA methylase, N-6 adenine-specific, conserved site	PROSITE	PS00092	N6_MTASE	coord: 160..166

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G1683.T1	Cucsat.G1683.T1	mRNA
Cucsat.G1683.T2	Cucsat.G1683.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0018022	peptidyl-lysine methylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016020	membrane
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0016279	protein-lysine N-methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity