Cucsat.G16656 (gene) Cucumber (B10) v3

Overview
NameCucsat.G16656
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionSmall COPII coat GTPase SAR1
Locationctg24: 2325875 .. 2328318 (+)
RNA-Seq ExpressionCucsat.G16656
SyntenyCucsat.G16656
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGAAAGAATAACAAAGAAAAGAAAGAAAGAAAGAAAGAGGAATTTAGTTTAATGGAAGGAATTGGATCCGAAATAGAGAAAGGAAAGGAGAGGTGTGTGCGTGTGTATGTGTGTGTATGTGTGTGTATGTGTGTGTGTGATTTGATTTCACATTAACACCCAAACAACAACAAAAAAGCTGTATTTTCGATCCCGTTTTTATTCTCTCATCTTCTTCTCCAATTCCCAATTCCCAATTCCCAATTCCCAAATTCCACATCCCTTTCTGGGTTTCAGATCGAACACCTTAACCATTCGGATTCCTCACTCCCCGACCTAGATCCCCTCCGATCTGCTTTCCCAATGTTCCTCATTGACTGGTTCTATGGCGTCTTGGCCTCCCTCGGTCTCTGGCAAAAGGAGGCCAAGATCCTCTTTCTCGGCCTCGATAATGCAGGAAAAACCACCCTCCTTCATATGTTGAAAGACGAGGTTTCAATCATTTCTTCCCTTTTTCCCATTTTTCTTTTCACGATCCATCTGTTGTTTTGTTTTCTTAATTGCGACGGATTTTCATTTCTTGCTCATGTTGTGCCTTGGTTATTTTTCTTTGTTTTTTTCTTTTTCTTTTTTCATGCTTTGCTTGTTCTAGAGGTTAGTTCAGCATCAGCCTACCCAGTACCCCACGTCCGAGGAGTTGAGTATTGGAAAAATCAAATTCAAGGCCTTTGATTTGGGTGGACATCAAATTGCTCGTAGAGTCTGGAAGGATTATTATGCCAAGGTACCCAATTTTCTCTAATTCAAGCTTATTGTATTTCCATTTCGTTTGATTTCTTTGCTTTTGTGGTGAGTTTTTTTTTTGGGCATTAAGCTCTTTGAAGACCAGTTTCCTATGAACTACCTCTCAATGCTTGTACTCTTTATCGATTGTCATATGTTCTTAGTCTGAGATTGTTTTGTAAAATATTCTGGACTTGGGTTTCCATCTAACTTGCACACTTATATAATTATGATTTATATGCATCTTCCCCCTCCATTGATGAAAGAAGAATGACACCGGTTCCTTTATTTTACTTATGTTGTAAGAAAAACCCGAGTCCTTCAGCTGCTTATTACTTGCTTAGTGTTTTGGATCTCTTGCACCTGTTGTTTATCTAACTTATTGTTGTGTGGTACTCATGTAATCAGTTATTTGTGTCAGTCTTTTGCTATCATAATATGCTCATCTGCCATTGACTTGTAAAGAATACACATCTAATAGCAGTTGCTTTAATTTGCGGGGGAGTGATATTTCCTACAGTTTAGTGTAATTTGAAAGAAATTTACCCAACTCTTGTGCCAAATGGAGTTTAACAATTTATCTTAGCCCTGAACCTTTGCAAGAAAGTTAACTAAGTTCTTAAAGCTATGGTCCACCTTGTTGATTATCTGTTTTTATTTATCTTTTAAAGCGAGAAAGCATTTTTTCAATGTCTGTTTTGTTGCCTGGTGTCATGTTTGACAATCTCCAGTTTGTAGTATTTCATTTCGAAGGATCTTGGTCATCCTTATAGTTTTAGGCAATTACATTGTGTGAAGTGCTATGTGGTGGATAGTTTTGCATATATTTTTCAGCTTCTCCAAATACTATGGTCCTTAGACATGAGTTCTAATTTGAAATGAAAAATGATATTACAGGTAGATGCTGTCGTGTACCTGGTTGATGCCTTTGACAAAGAGAGGTTTGCAGAATCAAAAAAGGAGCTCGATGCTCTTCTCTCTGATGAGTCTCTTGCTACTGTGCCATTCCTTATTTTGGGCAACAAGATTGACATTCCATATGCTGCCTCAGAGGATGAATTGCGGTATCACCTCGGTTTGACCAACTTCACCACAGGCAAGGGAAAAGTAAATCTGACTGATTCAAACGTACGTCCATTAGAGGTATTCATGTGCAGTATTGTTCGCAAAATGGGCTATGGCGATGGTTTCAAGTGGATGTCTCAGTACATCAAGTAGGTGAGATCAATAGGGTATGCCATACAGTTCCATGAATCGATGATATCCCACCGTCCAAAGCCCCTTCAAGTCGTCAACGTACGGATTGCAGTTTGATATTCAGTGTGGTAAGAGGGGCTTTAAGTTGAACTTTTTTTTTTTTTTTTTTTTTTTGCTTTCAGATGATGTGTGGAAGGAGGTACTGATGCTTACTATGTAAAAATTCACTTCCTTACCTCAAGTTTATTATTTTTGACATTCTTATCTCTCTTGTTATTCATCTGACTGGTTAGATGCGCTTTCCTAATTGTTGTTTTTCATCACTAGTTATAGGTTGAAGGTCTGAGAAACTAAAAACTTGGACTTGAGGGGCATACATGAACATTTGATTAAGATATTTTTTGGGGTTCTGAATAAGAAATCATAGATGATTGAAAGACTGTTTCCGTTTCTGTTTTCTTTTGTTTTTTTAATTCAAGTTTGTTTGTTTACTTTTCCACAATGATT

Coding sequence (CDS)

ATGTTCCTCATTGACTGGTTCTATGGCGTCTTGGCCTCCCTCGGTCTCTGGCAAAAGGAGGCCAAGATCCTCTTTCTCGGCCTCGATAATGCAGGAAAAACCACCCTCCTTCATATGTTGAAAGACGAGAGGTTAGTTCAGCATCAGCCTACCCAGTACCCCACGTCCGAGGAGTTGAGTATTGGAAAAATCAAATTCAAGGCCTTTGATTTGGGTGGACATCAAATTGCTCGTAGAGTCTGGAAGGATTATTATGCCAAGGTAGATGCTGTCGTGTACCTGGTTGATGCCTTTGACAAAGAGAGGTTTGCAGAATCAAAAAAGGAGCTCGATGCTCTTCTCTCTGATGAGTCTCTTGCTACTGTGCCATTCCTTATTTTGGGCAACAAGATTGACATTCCATATGCTGCCTCAGAGGATGAATTGCGGTATCACCTCGGTTTGACCAACTTCACCACAGGCAAGGGAAAAGTAAATCTGACTGATTCAAACGTACGTCCATTAGAGGTATTCATGTGCAGTATTGTTCGCAAAATGGGCTATGGCGATGGTTTCAAGTGGATGTCTCAGTACATCAAGTAG

Protein sequence

MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAFDKERFAESKKELDALLSDESLATVPFLILGNKIDIPYAASEDELRYHLGLTNFTTGKGKVNLTDSNVRPLEVFMCSIVRKMGYGDGFKWMSQYIK
Homology
[Close]
BLAST of Cucsat.G16656 vs. ExPASy Swiss-Prot
Match: O04834 (GTP-binding protein SAR1A OS=Arabidopsis thaliana OX=3702 GN=SAR1A PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.1e-17
Identity = 42/43 (97.67%), Postives = 43/43 (100.00%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 44
          MF+IDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE
Sbjct: 1  MFMIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 43

[Close]
BLAST of Cucsat.G16656 vs. ExPASy Swiss-Prot
Match: O04266 (GTP-binding protein SAR1A OS=Brassica campestris OX=3711 GN=SAR1A PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.4e-17
Identity = 42/43 (97.67%), Postives = 43/43 (100.00%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 44
          MF+IDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE
Sbjct: 1  MFVIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 43

[Close]
BLAST of Cucsat.G16656 vs. ExPASy Swiss-Prot
Match: P52884 (GTP-binding protein SAR2 OS=Solanum lycopersicum OX=4081 GN=SAR2 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 1.8e-17
Identity = 41/43 (95.35%), Postives = 43/43 (100.00%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 44
          MFL+DWFYGVLASLGLWQK+AKILFLGLDNAGKTTLLHMLKDE
Sbjct: 1  MFLVDWFYGVLASLGLWQKDAKILFLGLDNAGKTTLLHMLKDE 43

[Close]
BLAST of Cucsat.G16656 vs. ExPASy Swiss-Prot
Match: Q01474 (GTP-binding protein SAR1B OS=Arabidopsis thaliana OX=3702 GN=SAR1B PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 2.4e-17
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 44
          MFL DWFYG+LASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE
Sbjct: 1  MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 43

[Close]
BLAST of Cucsat.G16656 vs. ExPASy Swiss-Prot
Match: O04267 (GTP-binding protein SAR1B OS=Brassica campestris OX=3711 GN=SAR1B PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 2.4e-17
Identity = 41/43 (95.35%), Postives = 42/43 (97.67%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 44
          MFL DWFYG+LASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE
Sbjct: 1  MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 43

[Close]
BLAST of Cucsat.G16656 vs. NCBI nr
Match: KAF2578612.1 (hypothetical protein F2Q68_00002463 [Brassica cretica] >KAF2612353.1 hypothetical protein F2Q70_00009418 [Brassica cretica])

HSP 1 Score: 91.3 bits (225), Expect = 2.25e-21
Identity = 43/44 (97.73%), Postives = 44/44 (100.00%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEV 44
          MF+IDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEV
Sbjct: 1  MFVIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEV 44

[Close]
BLAST of Cucsat.G16656 vs. NCBI nr
Match: KAF7036149.1 (hypothetical protein CFC21_046895 [Triticum aestivum])

HSP 1 Score: 94.7 bits (234), Expect = 2.52e-21
Identity = 44/47 (93.62%), Postives = 47/47 (100.00%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVSII 47
          MFL+DWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVS++
Sbjct: 1  MFLVDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVSLL 47

[Close]
BLAST of Cucsat.G16656 vs. NCBI nr
Match: AIC33045.1 (putative GTP-binding protein sar1, partial [Panax ginseng])

HSP 1 Score: 90.1 bits (222), Expect = 4.65e-21
Identity = 42/43 (97.67%), Postives = 43/43 (100.00%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 43
          MFL+DWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE
Sbjct: 1  MFLLDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 43

[Close]
BLAST of Cucsat.G16656 vs. NCBI nr
Match: VAH75623.1 (unnamed protein product [Triticum turgidum subsp. durum])

HSP 1 Score: 90.5 bits (223), Expect = 5.94e-21
Identity = 42/43 (97.67%), Postives = 43/43 (100.00%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 43
          MFL+DWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE
Sbjct: 1  MFLVDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 43

[Close]
BLAST of Cucsat.G16656 vs. NCBI nr
Match: ONM31009.1 (GTP-binding protein SAR1B [Zea mays])

HSP 1 Score: 90.5 bits (223), Expect = 6.11e-21
Identity = 42/43 (97.67%), Postives = 43/43 (100.00%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 43
          MFL+DWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE
Sbjct: 1  MFLVDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDE 43

[Close]
BLAST of Cucsat.G16656 vs. ExPASy TrEMBL
Match: A0A287KR25 (Uncharacterized protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 3.04e-23
Identity = 47/55 (85.45%), Postives = 51/55 (92.73%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVSII---SSLFP 52
          MFL+DWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVS++   S +FP
Sbjct: 1  MFLVDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVSLLLFRSLVFP 55

[Close]
BLAST of Cucsat.G16656 vs. ExPASy TrEMBL
Match: A0A453EM49 (Uncharacterized protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 4.68e-23
Identity = 44/47 (93.62%), Postives = 47/47 (100.00%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVSII 47
          MFL+DWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVS++
Sbjct: 1  MFLVDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVSLL 47

[Close]
BLAST of Cucsat.G16656 vs. ExPASy TrEMBL
Match: A0A2P2KJ88 (GTP-binding protein SAR1A OS=Rhizophora mucronata OX=61149 PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 6.34e-23
Identity = 49/62 (79.03%), Postives = 52/62 (83.87%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVSIISSLFPIFLFTIHL 60
          MFL+DWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEV      F +FLF   +
Sbjct: 1  MFLVDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVPKFYLSFYLFLFFFWV 60

Query: 61 LF 62
          LF
Sbjct: 61 LF 62

[Close]
BLAST of Cucsat.G16656 vs. ExPASy TrEMBL
Match: A0A453EM36 (Uncharacterized protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.42e-22
Identity = 43/44 (97.73%), Postives = 44/44 (100.00%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEV 44
          MFL+DWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEV
Sbjct: 2  MFLVDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEV 45

[Close]
BLAST of Cucsat.G16656 vs. ExPASy TrEMBL
Match: A0A287QN97 (Uncharacterized protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 6.60e-22
Identity = 41/45 (91.11%), Postives = 44/45 (97.78%), Query Frame = 0 

Query: 1  MFLIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDEVS 45
          MFL+DWFYGVLASLG+WQKEAKILFLGLDNAGKTTLLHMLKDE+ 
Sbjct: 1  MFLVDWFYGVLASLGMWQKEAKILFLGLDNAGKTTLLHMLKDEIG 45

The following BLAST results are available for this feature:
BLAST of Cucsat.G16656 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O048341.1e-1797.67GTP-binding protein SAR1A OS=Arabidopsis thaliana OX=3702 GN=SAR1A PE=2 SV=1[more]
O042661.4e-1797.67GTP-binding protein SAR1A OS=Brassica campestris OX=3711 GN=SAR1A PE=2 SV=1[more]
P528841.8e-1795.35GTP-binding protein SAR2 OS=Solanum lycopersicum OX=4081 GN=SAR2 PE=2 SV=1[more]
Q014742.4e-1795.35GTP-binding protein SAR1B OS=Arabidopsis thaliana OX=3702 GN=SAR1B PE=1 SV=1[more]
O042672.4e-1795.35GTP-binding protein SAR1B OS=Brassica campestris OX=3711 GN=SAR1B PE=2 SV=1[more]
BLAST of Cucsat.G16656 vs. NCBI nr
Match NameE-valueIdentityDescription
KAF2578612.12.25e-2197.73hypothetical protein F2Q68_00002463 [Brassica cretica] >KAF2612353.1 hypothetica... [more]
KAF7036149.12.52e-2193.62hypothetical protein CFC21_046895 [Triticum aestivum][more]
AIC33045.14.65e-2197.67putative GTP-binding protein sar1, partial [Panax ginseng][more]
VAH75623.15.94e-2197.67unnamed protein product [Triticum turgidum subsp. durum][more]
ONM31009.16.11e-2197.67GTP-binding protein SAR1B [Zea mays][more]
BLAST of Cucsat.G16656 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A287KR253.04e-2385.45Uncharacterized protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1[more]
A0A453EM494.68e-2393.62Uncharacterized protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=3 S... [more]
A0A2P2KJ886.34e-2379.03GTP-binding protein SAR1A OS=Rhizophora mucronata OX=61149 PE=3 SV=1[more]
A0A453EM362.42e-2297.73Uncharacterized protein OS=Aegilops tauschii subsp. strangulata OX=200361 PE=3 S... [more]
A0A287QN976.60e-2291.11Uncharacterized protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006689Small GTPase superfamily, ARF/SAR typePRINTSPR00328SAR1GTPBPcoord: 77..102
score: 82.13
coord: 166..190
score: 79.53
coord: 22..45
score: 94.61
coord: 50..74
score: 86.12
coord: 122..143
score: 90.11
IPR006689Small GTPase superfamily, ARF/SAR typeSMARTSM00178sar_sub_1coord: 4..192
e-value: 1.0E-132
score: 456.9
IPR006689Small GTPase superfamily, ARF/SAR typePFAMPF00025Arfcoord: 8..192
e-value: 5.9E-65
score: 218.0
NoneNo IPR availableSMARTSM00177arf_sub_2coord: 4..193
e-value: 4.0E-17
score: 72.9
NoneNo IPR availablePANTHERPTHR45684:SF32PROTEIN SAR1A, PUTATIVE, EXPRESSED-RELATEDcoord: 1..193
NoneNo IPR availablePROSITEPS51417ARFcoord: 14..192
score: 15.570667
NoneNo IPR availablePROSITEPS51422SAR1coord: 14..192
score: 30.204769
NoneNo IPR availableCDDcd00879Sar1coord: 2..192
e-value: 1.33392E-131
score: 365.062
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 1..193
e-value: 2.9E-75
score: 253.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 17..192
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 19..135
e-value: 1.1E-14
score: 52.5
IPR006687Small GTPase superfamily, SAR1-typePANTHERPTHR45684RE74312Pcoord: 1..193

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G16656.T1Cucsat.G16656.T1mRNA
Cucsat.G16656.T2Cucsat.G16656.T2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding