Cucsat.G16544 (gene) Cucumber (B10) v3

Overview
NameCucsat.G16544
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionNodulation receptor kinase
Locationctg24: 429296 .. 431703 (+)
RNA-Seq ExpressionCucsat.G16544
SyntenyCucsat.G16544
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGGGAAAAAAACTATATAGTATACAATGTAATAATGAGTATGTTTGTTTTGAATCTAAGAAAAACTCATCATTGCCCTTAGACATAATTGAGCAAAGAGACATTCTCACTATGTTGAAATGGGAATTAAATTCAAGGAGTCTCTGCACAAACATAGCCGTCAATCAGCCATGGCCATCACATGGCTCACATCTGTCTTTTCTGCAAAATTTCCCCTTCTTTCTTTCTCTCTCTGCCTTCTGAAATGACCCATAATGCAATAACAACAATAATAATAACCTTATTCTTCTCTTGATCCAATGCTCATTCTAAAGCAATAGTTCTTCTTCTCTCTCCAAACAACTCCGAAAACAAGAAATGAACCAGACTTCAACTTCTCAGAAAACCATGAAACTGTTCTCTTCTGGCTTTTAAGGTTCTTCTTCTCTTGATTGAACCTCTTTTTTCTGTTCAAAGAAGGAGACTTTTCATCAAAAGAAGATGGCCCAGTTGATGAATCTAGCCATTGGAGTGATTTTAGTTGCAGTTTTAGTGGTTCTTCTTCTCATCTTCTTGAACAGAAGAGGAAAAGGGAAGGATAATGATGAATACAATGATATAGAGAGGAAACAAGAAAGGGAGAAAGGGGAAGAAGAAAGAGAAGAATTGGTGAGATTTCAAGGTGGACAAGATCTTACTATTGTGGATATCTTGGAAGCTCCCGGTGAAGTTATTGGGAAAGCCAATCATGGAACTTTGTATAAGGCTTATTTGCAAGGAAGCAATGCAGTTAGGTTACTGAGGTTTCTGAGGCCAGTTTGTACTGCTGGGCTTGAAGATTTTGTCAGTGAAATTGAGTTTTTGGGTTCCATAAGACACCCAAATTTGGTGCCTCTTTTGGGATTCTATTCAGGTCCAAGAGCTGAGAAGCTTCTCATTCATCCATTTTATAGAAGAGGAAATCTAGCTCAGTTCATTAGAGGTAAACAAAATCCATGCTCTTTCAGTTGTTTCTCTGTTTCTACATAAACAATGTGTTTTAGGACAACTATCTTCTGTGATATGGCTATGAAGCCTTTTGGAGTGAAACAAAAAAAGAAAATCACGAGGGTGTATGTCCAAAGTGGATAATATGCCATTGTTAAGATATCTAGAGGTCGTTGTCCCTAAGTGGATTTTTTTCCTACTTGCTTCCTTGTTATTTTCCTAGATAATGATCTTTCTTGTGAACCAATCAACAGAAATACAAGCATCTCTATAATATGTTTGTTAAACTAATGTACTTAACTTGATTCCGCTTGGCAGATGGGAACGGTGACTCGCACCGATGGGGAGTTATAAACAAGATCTCGGTTGGTATTGCGAAAGGGTTGGATCATCTTCATACTGGCTTGCAAAAGCCAACAATCCATGGGAATCTTCAATCAAAAAATGTTCTTTTGGACAGAAATTATGAACCGTATGTTTCTGATTTCGGTCTACATTTACTGTTGAATTCCTCATCTGCCCAAGAGGTAGTTGAAGCTTCAGCAGCCAATGGCTACAAAGCTCCTGAGCTGATCAAGATGAAGGATGCAACTGAGGAGACTGATATCTATAGCTATGGGGTGATATTACTCGAGTTACTTTCGGGGAAGGAACCTTTCAATGAAAAACCAACTGTTCCTGATGAGGATTTTTACCTGCCTAGCTTCATGAGAAATGCAGTTCTTGGACATAGAATTGCAGATTTGTTCCATCCAGAAATACTTCTATATAGCAGCATTGATGGAAATCATGTTACTGAAGAAAAGATTCTCAAATTCTTTCAGCTCGCTATGGCTTGTTGCTCTCCTTCTCCTGCTCTTAGACCAAGTATGAAGCAAGTACTACGGAAGCTCAATGAAATCAGAACGTAATATGGATTCTCGAGCATGTTACATCTCATAGGAGGAAGTTCTAAAAAAAAATGGGTATCAGTATAATTCTTGAGATCAACTAACTTCTCCATGTCCAACATGATTAAAGAGGGTATTGATTCTACTGTACATATATGGAATACCATTGGAGCAAAGAAGCTAAAGTTTTGGCATTTGAAGGGCTAAAGAAGCTGTACAAGTTGGGTATTTTTGAAGAATTTTTTGTTAAAAGATTCAATATGATTGAAGCCAAGTAGTGTTTTGCAAAATAGGTTTCTGCATTTTAGAACAAAGATTCAATAAAAACATATATCAGATAATTAGTGTATGAAAAATTCTCGCTTCTGATATAGTTTTACAAGAAACATGTTTTTGATAATAGTTTAAGTTATTCTTTCTTCCAAGATCGTGGATTTGTGAAAAGTTTCCCTAAGTACACAATAGCCTTGCTCATGCAAAATCAACAGTAAACCAATCCTTAAAAGAAATTTAAAAAAAAAAAGAAAGCAAAAGGCTGTATTTCAGAGCAA

Coding sequence (CDS)

ATGGCCCAGTTGATGAATCTAGCCATTGGAGTGATTTTAGTTGCAGTTTTAGTGGTTCTTCTTCTCATCTTCTTGAACAGAAGAGGAAAAGGGAAGGATAATGATGAATACAATGATATAGAGAGGAAACAAGAAAGGGAGAAAGGGGAAGAAGAAAGAGAAGAATTGGTGAGATTTCAAGGTGGACAAGATCTTACTATTGTGGATATCTTGGAAGCTCCCGGTGAAGTTATTGGGAAAGCCAATCATGGAACTTTGTATAAGGCTTATTTGCAAGGAAGCAATGCAGTTAGGTTACTGAGGTTTCTGAGGCCAGTTTGTACTGCTGGGCTTGAAGATTTTGTCAGTGAAATTGAGTTTTTGGGTTCCATAAGACACCCAAATTTGGTGCCTCTTTTGGGATTCTATTCAGGTCCAAGAGCTGAGAAGCTTCTCATTCATCCATTTTATAGAAGAGGAAATCTAGCTCAGTTCATTAGAGATGGGAACGGTGACTCGCACCGATGGGGAGTTATAAACAAGATCTCGGTTGGTATTGCGAAAGGGTTGGATCATCTTCATACTGGCTTGCAAAAGCCAACAATCCATGGGAATCTTCAATCAAAAAATGTTCTTTTGGACAGAAATTATGAACCGTATGTTTCTGATTTCGGTCTACATTTACTGTTGAATTCCTCATCTGCCCAAGAGGTAGTTGAAGCTTCAGCAGCCAATGGCTACAAAGCTCCTGAGCTGATCAAGATGAAGGATGCAACTGAGGAGACTGATATCTATAGCTATGGGGTGATATTACTCGAGTTACTTTCGGGGAAGGAACCTTTCAATGAAAAACCAACTGTTCCTGATGAGGATTTTTACCTGCCTAGCTTCATGAGAAATGCAGTTCTTGGACATAGAATTGCAGATTTGTTCCATCCAGAAATACTTCTATATAGCAGCATTGATGGAAATCATGTTACTGAAGAAAAGATTCTCAAATTCTTTCAGCTCGCTATGGCTTGTTGCTCTCCTTCTCCTGCTCTTAGACCAAGTATGAAGCAAGTACTACGGAAGCTCAATGAAATCAGAACGTAA

Protein sequence

MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQGGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEFLGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIAKGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGYKAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRIADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT
Homology
BLAST of Cucsat.G16544 vs. ExPASy Swiss-Prot
Match: P33543 (Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.3e-55
Identity = 138/356 (38.76%), Postives = 208/356 (58.43%), Query Frame = 0

Query: 7   LAIGVILVAVLVVLLLI--FLNRRGKGKDNDEYNDIERKQEREKGEEEREE--LVRFQGG 66
           L IG++  AV+V  LLI    N++ K     E +  E  +E E GE+E  E  LV FQGG
Sbjct: 304 LVIGLMSGAVVVASLLIGYLQNKKRKSSIESEDDLEEGDEEDEIGEKEGGEGKLVVFQGG 363

Query: 67  QDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEFLG 126
           ++LT+ D+L A G+V+ K ++GT+YKA L     +  LR LR          +  I  LG
Sbjct: 364 ENLTLDDVLNATGQVMEKTSYGTVYKAKLSDGGNI-ALRLLREGTCKDRSSCLPVIRQLG 423

Query: 127 SIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDG--NGDSHRWGVINKISVGIA 186
            IRH NLVPL  FY G R EKLLI+ +    +L   + +      +  W   +KI++GIA
Sbjct: 424 RIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHDLLHESKPRKPALNWARRHKIALGIA 483

Query: 187 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 246
           +GL +LHTG + P IHGN++SKNVL+D  +   +++FGL  ++  + A E+V  + ++GY
Sbjct: 484 RGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLTEFGLDKIMVQAVADEIVSQAKSDGY 543

Query: 247 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 306
           KAPEL KMK     +D+Y++G++LLE+L GK+P  +     +E   LPS ++ AVL    
Sbjct: 544 KAPELHKMKKCNPRSDVYAFGILLLEILMGKKP-GKSGRNGNEFVDLPSLVKAAVLEETT 603

Query: 307 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIR 357
            ++F  E +           EE ++   +LAM CC+P   +RPSM++V+++L E R
Sbjct: 604 MEVFDLEAMK----GIRSPMEEGLVHALKLAMGCCAPVTTVRPSMEEVVKQLEENR 653

BLAST of Cucsat.G16544 vs. ExPASy Swiss-Prot
Match: C0LGP9 (Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis thaliana OX=3702 GN=IMK3 PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 5.2e-52
Identity = 137/355 (38.59%), Postives = 195/355 (54.93%), Query Frame = 0

Query: 7   LAIGVILVAVLVVLLLIFLN----RRGKGKDNDEYNDIERKQEREKGEEEREELVRFQGG 66
           L + +ILV VL  LL    N    + G+          E+  E E G E   +LV F G 
Sbjct: 419 LIVMLILVCVLCCLLRKKANETKAKGGEAGPGAVAAKTEKGGEAEAGGETGGKLVHFDGP 478

Query: 67  QDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEFLG 126
              T  D+L A  E++GK+ +GT+YKA L+  + V + R LR   T   ++F +EI  LG
Sbjct: 479 MAFTADDLLCATAEIMGKSTYGTVYKATLEDGSQVAVKR-LREKITKSQKEFENEINVLG 538

Query: 127 SIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSH-RWGVINKISVGIAK 186
            IRHPNL+ L  +Y GP+ EKL++  +  RG+LA F+     D H  W     +  G+A+
Sbjct: 539 RIRHPNLLALRAYYLGPKGEKLVVFDYMSRGSLATFLHARGPDVHINWPTRMSLIKGMAR 598

Query: 187 GLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGYK 246
           GL +LHT      IHGNL S NVLLD N    +SD+GL  L+ +++   V+  + A GY+
Sbjct: 599 GLFYLHT--HANIIHGNLTSSNVLLDENITAKISDYGLSRLMTAAAGSSVIATAGALGYR 658

Query: 247 APELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRIA 306
           APEL K+K A  +TD+YS GVI+LELL+GK P      V      LP ++  AV      
Sbjct: 659 APELSKLKKANTKTDVYSLGVIILELLTGKSPSEALNGVD-----LPQWVATAVKEEWTN 718

Query: 307 DLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIR 357
           ++F  E+L     D N + +E IL   +LA+ C   +P+ RP  +QV+ +L EIR
Sbjct: 719 EVFDLELL----NDVNTMGDE-ILNTLKLALHCVDATPSTRPEAQQVMTQLGEIR 760

BLAST of Cucsat.G16544 vs. ExPASy Swiss-Prot
Match: Q9SCT4 (Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabidopsis thaliana OX=3702 GN=IMK2 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 8.3e-50
Identity = 126/361 (34.90%), Postives = 196/361 (54.29%), Query Frame = 0

Query: 7   LAIGVILVAVLV---VLLLIFLNRRGKGKDNDEYNDIERKQ-------EREKGEEEREEL 66
           +AIG +L  +L+   +LL   + +R   K  D  +    K            G E   +L
Sbjct: 462 IAIGALLAILLLLCCILLCCLIKKRAALKQKDGKDKTSEKTVSAGVAGTASAGGEMGGKL 521

Query: 67  VRFQGGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVS 126
           V F G    T  D+L A  E++GK+ +GT YKA L+  N V + R LR   T G+++F  
Sbjct: 522 VHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGNEVAVKR-LREKTTKGVKEFEG 581

Query: 127 EIEFLGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIR-DGNGDSHRWGVINKI 186
           E+  LG IRH NL+ L  +Y GP+ EKLL+  +  +G+L+ F+   G      W    KI
Sbjct: 582 EVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLSAFLHARGPETLIPWETRMKI 641

Query: 187 SVGIAKGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEAS 246
           + GI++GL HLH+   +  IH NL + N+LLD     +++D+GL  L+ +++A  V+  +
Sbjct: 642 AKGISRGLAHLHS--NENMIHENLTASNILLDEQTNAHIADYGLSRLMTAAAATNVIATA 701

Query: 247 AANGYKAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAV 306
              GY+APE  K+K+A+ +TD+YS G+I+LELL+GK P       P     LP ++ + V
Sbjct: 702 GTLGYRAPEFSKIKNASAKTDVYSLGIIILELLTGKSPGE-----PTNGMDLPQWVASIV 761

Query: 307 LGHRIADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEI 357
                 ++F  E++  +   G     +++L   +LA+ C  PSPA RP   QV+ +L EI
Sbjct: 762 KEEWTNEVFDLELMRETQSVG-----DELLNTLKLALHCVDPSPAARPEANQVVEQLEEI 809

BLAST of Cucsat.G16544 vs. ExPASy Swiss-Prot
Match: Q9LP77 (Probable inactive receptor kinase At1g48480 OS=Arabidopsis thaliana OX=3702 GN=RKL1 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 2.3e-44
Identity = 105/291 (36.08%), Postives = 167/291 (57.39%), Query Frame = 0

Query: 69  DILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEFLGSIRHPN 128
           D+L A  EV+GK   GT YKA L     V + R L+ V  A  ++F  +IE +G++ H N
Sbjct: 368 DLLRASAEVLGKGTFGTAYKAVLDAVTVVAVKR-LKDVMMAD-KEFKEKIELVGAMDHEN 427

Query: 129 LVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSH---RWGVINKISVGIAKGLDH 188
           LVPL  +Y   R EKLL++ F   G+L+  +    G       W V ++I++G A+GLD+
Sbjct: 428 LVPLRAYYFS-RDEKLLVYDFMPMGSLSALLHGNRGAGRSPLNWDVRSRIAIGAARGLDY 487

Query: 189 LHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGYKAPEL 248
           LH+     T HGN++S N+LL ++++  VSDFGL  L+ SS+       + A GY+APE+
Sbjct: 488 LHS-QGTSTSHGNIKSSNILLTKSHDAKVSDFGLAQLVGSSA----TNPNRATGYRAPEV 547

Query: 249 IKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRIADLFH 308
              K  +++ D+YS+GV+LLEL++GK P N    + +E   LP ++++        ++F 
Sbjct: 548 TDPKRVSQKGDVYSFGVVLLELITGKAPSNS--VMNEEGVDLPRWVKSVARDEWRREVFD 607

Query: 309 PEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIR 357
            E+L  ++ +     EE + +  QL + C S  P  RP M +V+RK+  +R
Sbjct: 608 SELLSLATDE-----EEMMAEMVQLGLECTSQHPDQRPEMSEVVRKMENLR 643

BLAST of Cucsat.G16544 vs. ExPASy Swiss-Prot
Match: Q9M9C5 (Probable leucine-rich repeat receptor-like protein kinase At1g68400 OS=Arabidopsis thaliana OX=3702 GN=At1g68400 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.2e-43
Identity = 110/323 (34.06%), Postives = 177/323 (54.80%), Query Frame = 0

Query: 38  NDIERKQEREKGEEEREELVRFQGGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAV 97
           N+ + +Q  +KG     ++V F+G +   + D+L A  E++GK   GT YKA L+  N V
Sbjct: 333 NNNQNQQVGDKG-----KMVFFEGTRRFELEDLLRASAEMLGKGGFGTAYKAVLEDGNEV 392

Query: 98  RLLRFLRPVCTAGLEDFVSEIEFLGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQ 157
            + R    V  AG ++F  ++E LG +RH NLV L  +Y   R EKLL++ +   G+L  
Sbjct: 393 AVKRLKDAVTVAGKKEFEQQMEVLGRLRHTNLVSLKAYYFA-REEKLLVYDYMPNGSLFW 452

Query: 158 FIRDGNGDSHR---WGVINKISVGIAKGLDHLHTGLQKPTI-HGNLQSKNVLLDRNYEPY 217
            +    G       W    KI+ G A+GL  +H   +   + HG+++S NVLLDR+    
Sbjct: 453 LLHGNRGPGRTPLDWTTRLKIAAGAARGLAFIHGSCKTLKLTHGDIKSTNVLLDRSGNAR 512

Query: 218 VSDFGLHLLLNSSSAQEVVEASAANGYKAPELIKMKDATEETDIYSYGVILLELLSGKEP 277
           VSDFGL +   S +       + +NGY+APELI  +  T+++D+YS+GV+LLE+L+GK P
Sbjct: 513 VSDFGLSIFAPSQT------VAKSNGYRAPELIDGRKHTQKSDVYSFGVLLLEILTGKCP 572

Query: 278 FNEKPTVPDEDFYLPSFMRNAVLGHRIADLFHPEILLYSSIDGNHVTEEKILKFFQLAMA 337
              +         LP ++++ V     A++F  E++ Y  I      EE+++   Q+AMA
Sbjct: 573 NMVETGHSGGAVDLPRWVQSVVREEWTAEVFDLELMRYKDI------EEEMVGLLQIAMA 632

Query: 338 CCSPSPALRPSMKQVLRKLNEIR 357
           C + +   RP M  V++ + +IR
Sbjct: 633 CTAVAADHRPKMGHVVKLIEDIR 637

BLAST of Cucsat.G16544 vs. NCBI nr
Match: XP_004146910.1 (putative kinase-like protein TMKL1 [Cucumis sativus] >KGN53161.1 hypothetical protein Csa_015214 [Cucumis sativus])

HSP 1 Score: 704 bits (1818), Expect = 8.96e-256
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60
           MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ
Sbjct: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60

Query: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120
           GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF
Sbjct: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120

Query: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180
           LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA
Sbjct: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180

Query: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240
           KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY
Sbjct: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240

Query: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300
           KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI
Sbjct: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300

Query: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357
           ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT
Sbjct: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357

BLAST of Cucsat.G16544 vs. NCBI nr
Match: XP_008453881.1 (PREDICTED: putative kinase-like protein TMKL1 [Cucumis melo] >KAA0044668.1 putative kinase-like protein TMKL1 [Cucumis melo var. makuwa] >TYK16914.1 putative kinase-like protein TMKL1 [Cucumis melo var. makuwa])

HSP 1 Score: 693 bits (1788), Expect = 3.35e-251
Identity = 349/357 (97.76%), Postives = 354/357 (99.16%), Query Frame = 0

Query: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60
           M Q++NLAIGVILVAVLVVLLL+FLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ
Sbjct: 1   MTQMVNLAIGVILVAVLVVLLLVFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60

Query: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120
           GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF
Sbjct: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120

Query: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180
           LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA
Sbjct: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180

Query: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240
           KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY
Sbjct: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240

Query: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300
           KAPELIKMKDATEETDIYSYGVILLELLSGKEP NEKPTVPDEDFYLP+FMRNAVLGHRI
Sbjct: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPINEKPTVPDEDFYLPNFMRNAVLGHRI 300

Query: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357
           ADLFHPEILLYS+IDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEI T
Sbjct: 301 ADLFHPEILLYSNIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIGT 357

BLAST of Cucsat.G16544 vs. NCBI nr
Match: XP_038881903.1 (putative kinase-like protein TMKL1 [Benincasa hispida])

HSP 1 Score: 675 bits (1741), Expect = 4.88e-244
Identity = 339/357 (94.96%), Postives = 348/357 (97.48%), Query Frame = 0

Query: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60
           M QL+NLA+GV+LVAVLV+L LIFLNRRGKGKD DEYNDIERKQ+R+K EEEREELV FQ
Sbjct: 1   MTQLLNLALGVVLVAVLVILFLIFLNRRGKGKDIDEYNDIERKQDRDKEEEEREELVCFQ 60

Query: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120
           GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSN VRLLRFLRPVCTAGLEDFVSEIEF
Sbjct: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNTVRLLRFLRPVCTAGLEDFVSEIEF 120

Query: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180
           LGSIRH NLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA
Sbjct: 121 LGSIRHQNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180

Query: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240
           KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNS SAQE++EASAANGY
Sbjct: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSPSAQEILEASAANGY 240

Query: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300
           KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLP+FMRNAVLGHRI
Sbjct: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPNFMRNAVLGHRI 300

Query: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357
           ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEI T
Sbjct: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIGT 357

BLAST of Cucsat.G16544 vs. NCBI nr
Match: KAG6576821.1 (putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 630 bits (1626), Expect = 4.44e-226
Identity = 317/357 (88.80%), Postives = 333/357 (93.28%), Query Frame = 0

Query: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60
           M Q +NLA+GV  V  L VLLLIFLNRRGKGKD D +ND+ER Q+  KG EERE+L+ FQ
Sbjct: 29  MTQFLNLALGVFFVLGLAVLLLIFLNRRGKGKDGDGFNDLERNQQSVKGAEEREDLLTFQ 88

Query: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120
           GGQDLTI+DILEAPGEVIGKANHGTLYKAYLQGS+ VRLLRFLRPVCTAG +DFVSEIEF
Sbjct: 89  GGQDLTILDILEAPGEVIGKANHGTLYKAYLQGSSTVRLLRFLRPVCTAGHDDFVSEIEF 148

Query: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180
           LGSIRHPNLVPLLGFYSGPR EKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKIS+GIA
Sbjct: 149 LGSIRHPNLVPLLGFYSGPRTEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISIGIA 208

Query: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240
           KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEV+E  AANGY
Sbjct: 209 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVLEGLAANGY 268

Query: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300
           KAPE+IKMKDATEETD+YSYGVILLELLSGKEP NEKP   DEDFYLP+FMRNAVLGHRI
Sbjct: 269 KAPEVIKMKDATEETDVYSYGVILLELLSGKEPVNEKPAF-DEDFYLPNFMRNAVLGHRI 328

Query: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357
           ADLFHPEILLYSSIDGN+VTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEI T
Sbjct: 329 ADLFHPEILLYSSIDGNNVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIGT 384

BLAST of Cucsat.G16544 vs. NCBI nr
Match: XP_022985481.1 (putative kinase-like protein TMKL1 [Cucurbita maxima])

HSP 1 Score: 630 bits (1624), Expect = 8.95e-226
Identity = 315/357 (88.24%), Postives = 335/357 (93.84%), Query Frame = 0

Query: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60
           M Q +NLA+GV+ V  L V+LLIFLNRRGKGK+ D +ND+ER Q+  KG EERE+L+ FQ
Sbjct: 29  MTQFLNLALGVVFVLGLAVVLLIFLNRRGKGKNGDGFNDLERNQQSVKGAEEREDLLTFQ 88

Query: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120
           GGQDLTI+DILEAPGEVIGKANHGTLYKAYLQGS+ VRLLRFLRPVCTAG +DFVSEIEF
Sbjct: 89  GGQDLTILDILEAPGEVIGKANHGTLYKAYLQGSSTVRLLRFLRPVCTAGHDDFVSEIEF 148

Query: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180
           LGSIRHPNLVPLLGFYSGPR EKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA
Sbjct: 149 LGSIRHPNLVPLLGFYSGPRTEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 208

Query: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240
           KGLDHLHTGLQKPTIHGNLQSKN+LLDRNYEPYVSDFGLHLLLNSSSAQEV+E  AANGY
Sbjct: 209 KGLDHLHTGLQKPTIHGNLQSKNILLDRNYEPYVSDFGLHLLLNSSSAQEVLEGLAANGY 268

Query: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300
           KAPE+IKMKDATEETD+YSYGVILLELLSGKEP NEKP   DEDFYLP+FMRNAVLGHRI
Sbjct: 269 KAPEVIKMKDATEETDVYSYGVILLELLSGKEPVNEKPAF-DEDFYLPNFMRNAVLGHRI 328

Query: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357
           ADLFHPEILLYSSIDGN+VTEEKILKFFQLAMACCSPSPALRPSMKQVL+KLNEIRT
Sbjct: 329 ADLFHPEILLYSSIDGNNVTEEKILKFFQLAMACCSPSPALRPSMKQVLQKLNEIRT 384

BLAST of Cucsat.G16544 vs. ExPASy TrEMBL
Match: A0A0A0KZ69 (Nodulation receptor kinase OS=Cucumis sativus OX=3659 GN=Csa_4G022965 PE=4 SV=1)

HSP 1 Score: 704 bits (1818), Expect = 4.34e-256
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60
           MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ
Sbjct: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60

Query: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120
           GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF
Sbjct: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120

Query: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180
           LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA
Sbjct: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180

Query: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240
           KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY
Sbjct: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240

Query: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300
           KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI
Sbjct: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300

Query: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357
           ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT
Sbjct: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357

BLAST of Cucsat.G16544 vs. ExPASy TrEMBL
Match: A0A5D3D101 (Putative kinase-like protein TMKL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00500 PE=4 SV=1)

HSP 1 Score: 693 bits (1788), Expect = 1.62e-251
Identity = 349/357 (97.76%), Postives = 354/357 (99.16%), Query Frame = 0

Query: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60
           M Q++NLAIGVILVAVLVVLLL+FLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ
Sbjct: 1   MTQMVNLAIGVILVAVLVVLLLVFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60

Query: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120
           GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF
Sbjct: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120

Query: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180
           LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA
Sbjct: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180

Query: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240
           KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY
Sbjct: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240

Query: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300
           KAPELIKMKDATEETDIYSYGVILLELLSGKEP NEKPTVPDEDFYLP+FMRNAVLGHRI
Sbjct: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPINEKPTVPDEDFYLPNFMRNAVLGHRI 300

Query: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357
           ADLFHPEILLYS+IDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEI T
Sbjct: 301 ADLFHPEILLYSNIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIGT 357

BLAST of Cucsat.G16544 vs. ExPASy TrEMBL
Match: A0A1S3BXA5 (putative kinase-like protein TMKL1 OS=Cucumis melo OX=3656 GN=LOC103494468 PE=4 SV=1)

HSP 1 Score: 693 bits (1788), Expect = 1.62e-251
Identity = 349/357 (97.76%), Postives = 354/357 (99.16%), Query Frame = 0

Query: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60
           M Q++NLAIGVILVAVLVVLLL+FLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ
Sbjct: 1   MTQMVNLAIGVILVAVLVVLLLVFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60

Query: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120
           GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF
Sbjct: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120

Query: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180
           LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA
Sbjct: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180

Query: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240
           KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY
Sbjct: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240

Query: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300
           KAPELIKMKDATEETDIYSYGVILLELLSGKEP NEKPTVPDEDFYLP+FMRNAVLGHRI
Sbjct: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPINEKPTVPDEDFYLPNFMRNAVLGHRI 300

Query: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357
           ADLFHPEILLYS+IDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEI T
Sbjct: 301 ADLFHPEILLYSNIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIGT 357

BLAST of Cucsat.G16544 vs. ExPASy TrEMBL
Match: A0A6J1J8A3 (putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111483478 PE=4 SV=1)

HSP 1 Score: 630 bits (1624), Expect = 4.33e-226
Identity = 315/357 (88.24%), Postives = 335/357 (93.84%), Query Frame = 0

Query: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60
           M Q +NLA+GV+ V  L V+LLIFLNRRGKGK+ D +ND+ER Q+  KG EERE+L+ FQ
Sbjct: 29  MTQFLNLALGVVFVLGLAVVLLIFLNRRGKGKNGDGFNDLERNQQSVKGAEEREDLLTFQ 88

Query: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120
           GGQDLTI+DILEAPGEVIGKANHGTLYKAYLQGS+ VRLLRFLRPVCTAG +DFVSEIEF
Sbjct: 89  GGQDLTILDILEAPGEVIGKANHGTLYKAYLQGSSTVRLLRFLRPVCTAGHDDFVSEIEF 148

Query: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180
           LGSIRHPNLVPLLGFYSGPR EKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA
Sbjct: 149 LGSIRHPNLVPLLGFYSGPRTEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 208

Query: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240
           KGLDHLHTGLQKPTIHGNLQSKN+LLDRNYEPYVSDFGLHLLLNSSSAQEV+E  AANGY
Sbjct: 209 KGLDHLHTGLQKPTIHGNLQSKNILLDRNYEPYVSDFGLHLLLNSSSAQEVLEGLAANGY 268

Query: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300
           KAPE+IKMKDATEETD+YSYGVILLELLSGKEP NEKP   DEDFYLP+FMRNAVLGHRI
Sbjct: 269 KAPEVIKMKDATEETDVYSYGVILLELLSGKEPVNEKPAF-DEDFYLPNFMRNAVLGHRI 328

Query: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357
           ADLFHPEILLYSSIDGN+VTEEKILKFFQLAMACCSPSPALRPSMKQVL+KLNEIRT
Sbjct: 329 ADLFHPEILLYSSIDGNNVTEEKILKFFQLAMACCSPSPALRPSMKQVLQKLNEIRT 384

BLAST of Cucsat.G16544 vs. ExPASy TrEMBL
Match: A0A6J1CI76 (putative kinase-like protein TMKL1 OS=Momordica charantia OX=3673 GN=LOC111011407 PE=4 SV=1)

HSP 1 Score: 627 bits (1618), Expect = 1.21e-225
Identity = 314/357 (87.96%), Postives = 339/357 (94.96%), Query Frame = 0

Query: 1   MAQLMNLAIGVILVAVLVVLLLIFLNRRGKGKDNDEYNDIERKQEREKGEEEREELVRFQ 60
           M Q++NLA+G +LV +LV+LL++FLNRRG+GKD + YNDIE KQ+REKG+EEREELV F+
Sbjct: 1   MTQMLNLALGAVLVTILVILLVVFLNRRGRGKD-EYYNDIESKQQREKGDEEREELVSFE 60

Query: 61  GGQDLTIVDILEAPGEVIGKANHGTLYKAYLQGSNAVRLLRFLRPVCTAGLEDFVSEIEF 120
           GGQDLTIVD+LEAPGEVIGKANHGTLYKAYLQGS+ VRLLRFLRPVCT+GL++FVSEI+F
Sbjct: 61  GGQDLTIVDVLEAPGEVIGKANHGTLYKAYLQGSSTVRLLRFLRPVCTSGLQEFVSEIQF 120

Query: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNGDSHRWGVINKISVGIA 180
           LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGN DSHRWGVI KISVGIA
Sbjct: 121 LGSIRHPNLVPLLGFYSGPRAEKLLIHPFYRRGNLAQFIRDGNSDSHRWGVILKISVGIA 180

Query: 181 KGLDHLHTGLQKPTIHGNLQSKNVLLDRNYEPYVSDFGLHLLLNSSSAQEVVEASAANGY 240
           KGLDHLHTGL+KP IHGNLQSKNVLLDRN+EPYVSDFGLHLLLNS SAQEV+EASAANGY
Sbjct: 181 KGLDHLHTGLKKPIIHGNLQSKNVLLDRNFEPYVSDFGLHLLLNSPSAQEVLEASAANGY 240

Query: 241 KAPELIKMKDATEETDIYSYGVILLELLSGKEPFNEKPTVPDEDFYLPSFMRNAVLGHRI 300
           KAPELIKMKDA EETD YSYGVILLELLSG+EP NEKPT PDEDFYLPSFMRNAVLGHRI
Sbjct: 241 KAPELIKMKDAAEETDAYSYGVILLELLSGREPINEKPT-PDEDFYLPSFMRNAVLGHRI 300

Query: 301 ADLFHPEILLYSSIDGNHVTEEKILKFFQLAMACCSPSPALRPSMKQVLRKLNEIRT 357
           ADLFHPEILLYSSIDGN VTEEKIL+FFQLAMACCSPSPALRPSMKQVLRKL+EI T
Sbjct: 301 ADLFHPEILLYSSIDGNAVTEEKILQFFQLAMACCSPSPALRPSMKQVLRKLDEIGT 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P335431.3e-5538.76Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1... [more]
C0LGP95.2e-5238.59Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis th... [more]
Q9SCT48.3e-5034.90Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabi... [more]
Q9LP772.3e-4436.08Probable inactive receptor kinase At1g48480 OS=Arabidopsis thaliana OX=3702 GN=R... [more]
Q9M9C51.2e-4334.06Probable leucine-rich repeat receptor-like protein kinase At1g68400 OS=Arabidops... [more]
Match NameE-valueIdentityDescription
XP_004146910.18.96e-256100.00putative kinase-like protein TMKL1 [Cucumis sativus] >KGN53161.1 hypothetical pr... [more]
XP_008453881.13.35e-25197.76PREDICTED: putative kinase-like protein TMKL1 [Cucumis melo] >KAA0044668.1 putat... [more]
XP_038881903.14.88e-24494.96putative kinase-like protein TMKL1 [Benincasa hispida][more]
KAG6576821.14.44e-22688.80putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022985481.18.95e-22688.24putative kinase-like protein TMKL1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0A0KZ694.34e-256100.00Nodulation receptor kinase OS=Cucumis sativus OX=3659 GN=Csa_4G022965 PE=4 SV=1[more]
A0A5D3D1011.62e-25197.76Putative kinase-like protein TMKL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BXA51.62e-25197.76putative kinase-like protein TMKL1 OS=Cucumis melo OX=3656 GN=LOC103494468 PE=4 ... [more]
A0A6J1J8A34.33e-22688.24putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111483478 P... [more]
A0A6J1CI761.21e-22587.96putative kinase-like protein TMKL1 OS=Momordica charantia OX=3673 GN=LOC11101140... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 34..54
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 151..357
e-value: 2.6E-43
score: 149.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 41..149
e-value: 2.0E-11
score: 45.6
NoneNo IPR availablePANTHERPTHR48008:SF13PROTEIN KINASE SUPERFAMILY PROTEINcoord: 5..356
NoneNo IPR availablePANTHERPTHR48008LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATEDcoord: 5..356
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 76..351
e-value: 1.0E-31
score: 110.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 72..356
score: 30.025576
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 75..352

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G16544.T1Cucsat.G16544.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity