Cucsat.G16467 (gene) Cucumber (B10) v3

Overview
NameCucsat.G16467
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionallene oxide synthase 3-like
Locationctg2387: 331763 .. 333779 (+)
RNA-Seq ExpressionCucsat.G16467
SyntenyCucsat.G16467
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAAATAGGCACAGGTGGTTGGGGCTACTTATATTAAAAATTGTGTTGCAAATTCCAATGAACGCATTTGGAATAAAATGAATACAAAGACAGAGGGATACACACACACACACACACACAACATTGGATTAAACATGTGCTTCCTAAATATCATTCATTAAAATCCTATTCCTCTCCCCATCTTCTCCGTTCTCCAATAACTTTTTTTAAGAAAAAAATGACTTCATCTTCTTCAGAACACCCACAAATTCCCCTCCCTCTGAAACCCATCCCCGGCAGCTATGGCTTCCCCATCTTTGGACCCATCATAGATCGCTACCATTACTTCTACATCCAAGGCAGAGAAACCTTCTTCCGATCCCGAATGGCCAAGTACAATTCCACTGTGTTCCGTACCAACATGCCGCCAGGTCCCTTCATCTCTTCCAATTCCAAAGTAATCGTCCTTCTCGACGCCCTCACTTTTCCGATCCTCTTCGACACCACTAAAGTTGAGAAACGCAACGTTCTCGATGGAACTTACATGCCCTCCCTTGCCTTCACTGGAGGCATTCGTACTTGTGCTTTCTTAGACCCATCTGAAACAGAGCACTCTGTTCTCAAACGCCATTTCCTCAAATTTCTTGCCTCTCGTCACCACCAATTCATTCCTCTCTTTCGAACCTCCATCTCCGAGATGTTTGATAAACTTGAAAAGGAATTACAAAACAATAACGTCGCCAATTTTAATCCTATCAGTGATTACGCATCCTTTGATTTCATTTTCCGATTGCTTTCCGATCGCAGCCCCGATAAGAATTTCAGCTCCGAGGGACCGGGTTTAGTCGACCGATGGCTTACTATGCAGCTCGCTCCATTAGCTACACTTGGTTTGCCCAAAATTTTCAGCTGCTTTGAAGATCTCATCATCCATACTTTCCGTTTACCGTTCGCACTCGTTAAAAGCGCTTACAGGTAAATGAATGAAAACTGAAATCAACTTCTTTCTTAAATTACTAAAAACTATCTAGAATCAGAACCACAAACTGATTAATGCTGTATTACAGGAAGCTCTACGAATCGTTTTACGAGTCGTCAGGTTCATTTCTAGACGAAGCCGAGAAACAGGGAATAAACAGAGAGAAAGCATGTCATAATTTAGTGTTTTTAGCTGGATTCAACGCATACGCCGGAATGAAAGTGTTGTTGCCGATTCTGCTGAACTGGGTTGGATCTGCCGGCGAGGAGCTTCATCGGAAACTAGTGGGCGAAATCAGAGCCGCCGTGAAGATAGACGGGGGAATAACATTCGGGGCTTTAGAGAAGATGAGTCTGCTGAAATCCGTGGTGTACGAGGTTCTGAGGATCGACCCGCCGGTGCCGTACCAATACGCAAAGGCGAAACAAGACATTGTGATAGAGAGCCATGATTCTGCTTTCGAGATCAAGAAAGGGGAAATGATTTTTGGGTATCAGCCGATTGCGACAAAAGATCCGAAAGTGTTTGAGAATGCGGAGGAGTTTGTGGGCGAAAGGTTCGTGGGCGAGAAAGGGGAGAAGCTTTTGAAGTATGTTTATTGGTCAAACGGTCGGGAGACGGAGGAGCCAACGGCGGAGAACAAGCAGTGTCCGGCGAGGGATCTGGTGGTGCTGATGTGCAGAGTAGTGTTGGTGGAACTTTTCCTCCGTTATGAGACGTTCACCGTCGAGGGAACCCGGTCGTCGTTGGGTTGGTCTGTGAAGGTGAAATCGTTGACTAAAGCCTAATCGCTATCAACGGTTGCGCCAGCTTCCTCTAAGCTCTACGCCTTTCAAACAAATAAAGACTGAAATTCATTTTTAGTCCTAATTACTCTCTTCAATTTTTAAATTATGACCTTATTTCCAATTTCTCTTCTTTTTTTTTTTTTTTTTTTTTTTGAACACTCTATATTGCATTGTGAGAATAAATTTTAGTAATATATCATACATAAATATATATTGATAATTATTTATTAAGCTAAGTTGATCATGTTTTAAATTAGAATCATTGGTGATT

Coding sequence (CDS)

ATGACTTCATCTTCTTCAGAACACCCACAAATTCCCCTCCCTCTGAAACCCATCCCCGGCAGCTATGGCTTCCCCATCTTTGGACCCATCATAGATCGCTACCATTACTTCTACATCCAAGGCAGAGAAACCTTCTTCCGATCCCGAATGGCCAAGTACAATTCCACTGTGTTCCGTACCAACATGCCGCCAGGTCCCTTCATCTCTTCCAATTCCAAAGTAATCGTCCTTCTCGACGCCCTCACTTTTCCGATCCTCTTCGACACCACTAAAGTTGAGAAACGCAACGTTCTCGATGGAACTTACATGCCCTCCCTTGCCTTCACTGGAGGCATTCGTACTTGTGCTTTCTTAGACCCATCTGAAACAGAGCACTCTGTTCTCAAACGCCATTTCCTCAAATTTCTTGCCTCTCGTCACCACCAATTCATTCCTCTCTTTCGAACCTCCATCTCCGAGATGTTTGATAAACTTGAAAAGGAATTACAAAACAATAACGTCGCCAATTTTAATCCTATCAGTGATTACGCATCCTTTGATTTCATTTTCCGATTGCTTTCCGATCGCAGCCCCGATAAGAATTTCAGCTCCGAGGGACCGGGTTTAGTCGACCGATGGCTTACTATGCAGCTCGCTCCATTAGCTACACTTGGTTTGCCCAAAATTTTCAGCTGCTTTGAAGATCTCATCATCCATACTTTCCGTTTACCGTTCGCACTCGTTAAAAGCGCTTACAGGAAGCTCTACGAATCGTTTTACGAGTCGTCAGGTTCATTTCTAGACGAAGCCGAGAAACAGGGAATAAACAGAGAGAAAGCATGTCATAATTTAGTGTTTTTAGCTGGATTCAACGCATACGCCGGAATGAAAGTGTTGTTGCCGATTCTGCTGAACTGGGTTGGATCTGCCGGCGAGGAGCTTCATCGGAAACTAGTGGGCGAAATCAGAGCCGCCGTGAAGATAGACGGGGGAATAACATTCGGGGCTTTAGAGAAGATGAGTCTGCTGAAATCCGTGGTGTACGAGGTTCTGAGGATCGACCCGCCGGTGCCGTACCAATACGCAAAGGCGAAACAAGACATTGTGATAGAGAGCCATGATTCTGCTTTCGAGATCAAGAAAGGGGAAATGATTTTTGGGTATCAGCCGATTGCGACAAAAGATCCGAAAGTGTTTGAGAATGCGGAGGAGTTTGTGGGCGAAAGGTTCGTGGGCGAGAAAGGGGAGAAGCTTTTGAAGTATGTTTATTGGTCAAACGGTCGGGAGACGGAGGAGCCAACGGCGGAGAACAAGCAGTGTCCGGCGAGGGATCTGGTGGTGCTGATGTGCAGAGTAGTGTTGGTGGAACTTTTCCTCCGTTATGAGACGTTCACCGTCGAGGGAACCCGGTCGTCGTTGGGTTGGTCTGTGAAGGTGAAATCGTTGACTAAAGCCTAA

Protein sequence

MTSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRTNMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDPSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFDFIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFALVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWVGSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNGRETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA
Homology
BLAST of Cucsat.G16467 vs. ExPASy Swiss-Prot
Match: Q8GZP5 (Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 1.1e-153
Identity = 266/480 (55.42%), Postives = 351/480 (73.12%), Query Frame = 0

Query: 1   MTSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRT 60
           +T  + E     LP+K IPG YG P FG I DRY + Y QG + FFRSRM KY+STVFRT
Sbjct: 11  ITMDTKESSIPSLPMKEIPGDYGVPFFGAIKDRYDFHYNQGADEFFRSRMKKYDSTVFRT 70

Query: 61  NMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDP 120
           N+PPGPF + NSKV+VL+DA+++PILFD ++V+K N  +GT+M S +F GG + C FL  
Sbjct: 71  NVPPGPFNARNSKVVVLVDAVSYPILFDNSQVDKENYFEGTFMSSPSFNGGYKVCGFLGT 130

Query: 121 SETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFD 180
           S+ +H+ LK  FL  L   H +FIP+F TSI+ MF  LEKEL     + FNPI D  SF+
Sbjct: 131 SDPKHTTLKGLFLSTLTRLHDKFIPIFTTSITSMFTSLEKELSEKGTSYFNPIGDNLSFE 190

Query: 181 FIFRLLSD-RSP-DKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPF 240
           F+FRL  + ++P D +    GP +VD+W+ +QLAPL +LGL  + +  EDL++HTF LP+
Sbjct: 191 FLFRLFCEGKNPIDTSVGPNGPKIVDKWVFLQLAPLISLGLKFVPNFLEDLVLHTFPLPY 250

Query: 241 ALVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLN 300
            LVK  ++KLY +FY S    LDEAEK G+ R++ACHN VFLAGFN+Y G+KV  P L+ 
Sbjct: 251 ILVKRDHQKLYNAFYNSMKDILDEAEKLGVKRDEACHNFVFLAGFNSYGGLKVFFPSLIK 310

Query: 301 WVGSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAK 360
           W+G++G  LH +LV EIR AVK  GG+T  A++KM L+KSVVYE LR+DPPVP+Q  KA+
Sbjct: 311 WIGTSGPSLHARLVKEIRTAVKEAGGVTLSAIDKMPLVKSVVYETLRMDPPVPFQTVKAR 370

Query: 361 QDIVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWS 420
           ++I+I +H+S+F IKK E+IFGYQP+ATKD KVF+NAEEF  +RFVG  GEKLLKYVYWS
Sbjct: 371 KNIIITNHESSFLIKKDELIFGYQPLATKDSKVFKNAEEFNPDRFVG-GGEKLLKYVYWS 430

Query: 421 NGRETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 479
           NG+E + P+  +KQCP +DL+VLM R+++VE F+RY+TF VE  +  LG  V  KSLTKA
Sbjct: 431 NGKEIDNPSVNDKQCPGKDLIVLMGRLLVVEFFMRYDTFEVEFGKLLLGSKVTFKSLTKA 489

BLAST of Cucsat.G16467 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 4.4e-147
Identity = 258/480 (53.75%), Postives = 343/480 (71.46%), Query Frame = 0

Query: 3   SSSSEHP--QIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRT 62
           SSSS  P  Q  LP + +PG YG P+ GP  DR  YFY QG+  FF+SR+ K+ STVFRT
Sbjct: 56  SSSSSPPVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQSTVFRT 115

Query: 63  NMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDP 122
           NMPPGPFIS N  V+VLLD  +FP+LFD +KVEK+++  GT+MPS   TGG R  ++LDP
Sbjct: 116 NMPPGPFISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRVLSYLDP 175

Query: 123 SETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFD 182
           SE  H+ LK+     L+SR ++ IP F  S SE+F+ LE EL     A  N  +D A+F+
Sbjct: 176 SEPNHAKLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGKAGLNAANDQAAFN 235

Query: 183 FIFRLLSDRSP-DKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 242
           F+ R L   +P D    ++GP L+ +W+  QL PL  LGLPK+    EDL++HTFRLP A
Sbjct: 236 FLARSLYGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPKV---LEDLVMHTFRLPPA 295

Query: 243 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNW 302
           LVK  Y++LY  FYE+S S LDEAEK GI+RE+ACHNL+F   FN++ G+K+  P +L W
Sbjct: 296 LVKKDYQRLYNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIFFPNMLKW 355

Query: 303 VGSAGEELHRKLVGEIRAAVKIDGG-ITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAK 362
           +G AG +LH +L  EIR+ +  + G +T  A+EKM L+KSVVYE LRI+PPV  QY +AK
Sbjct: 356 IGRAGAKLHSQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVASQYGRAK 415

Query: 363 QDIVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWS 422
            D+VIESHD++FEIK+GE+++GYQP ATKDPK+F+ +EEFV +RF+GE+GEKLLK+V WS
Sbjct: 416 HDMVIESHDASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKLLKHVLWS 475

Query: 423 NGRETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 479
           NG ETE  +  NKQC  +D VVL+ R++LVELFLRY++F +E   S LG ++ + SL +A
Sbjct: 476 NGSETENASINNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAITLTSLRRA 532

BLAST of Cucsat.G16467 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 505.0 bits (1299), Expect = 9.4e-142
Identity = 247/468 (52.78%), Postives = 332/468 (70.94%), Query Frame = 0

Query: 13  LPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRTNMPPGPFISSNS 72
           LP++ IPG+YG PI GPI DR+ YFY QG E FF+SR+ KYNSTV+R NMPPG FI+ N 
Sbjct: 52  LPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENP 111

Query: 73  KVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDPSETEHSVLKRHF 132
           +V+ LLD  +FP+LFD  KVEK+++  GTYMPS   TGG R  ++LDPSE +H  LK   
Sbjct: 112 QVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLL 171

Query: 133 LKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFDFIFRLLSDRSP- 192
              L S  ++  P F+ + SE+FD LEKEL     A+F   SD  +F+F+ R     +P 
Sbjct: 172 FFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPA 231

Query: 193 DKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFALVKSAYRKLYES 252
           D    ++ PGL+ +W+   L PL ++GLP++    E+ +IHTF LP ALVKS Y++LYE 
Sbjct: 232 DTKLKADAPGLITKWVLFNLHPLLSIGLPRV---IEEPLIHTFSLPPALVKSDYQRLYEF 291

Query: 253 FYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWVGSAGEELHRKL 312
           F ES+G  L EA+K GI+RE+A HNL+F   FN + GMK+L P ++  +G AG ++H +L
Sbjct: 292 FLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRL 351

Query: 313 VGEIRAAVKIDGG-ITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAF 372
             EIR+ +K +GG +T GA+EKM L KSVVYE LR +PPV  QY +AK+D+VIESHD+AF
Sbjct: 352 AEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAF 411

Query: 373 EIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNGRETEEPTAEN 432
           ++K GEM++GYQP+AT+DPK+F+ A+EFV ERFVGE+GEKLL++V WSNG ETE PT  N
Sbjct: 412 KVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGN 471

Query: 433 KQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 479
           KQC  +D VVL+ R+ ++E+F RY++F +E   S LG SV   SL KA
Sbjct: 472 KQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKA 516

BLAST of Cucsat.G16467 vs. ExPASy Swiss-Prot
Match: Q9LLB0 (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 PE=1 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 2.1e-141
Identity = 247/470 (52.55%), Postives = 328/470 (69.79%), Query Frame = 0

Query: 11  IPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRTNMPPGPFISS 70
           I L  + IPG YG P  GP  DR  YFY QG+  FF SR+AKY ST+FRTNMPPGPFI+S
Sbjct: 44  IKLSTRTIPGDYGLPGIGPWKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMPPGPFITS 103

Query: 71  NSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDPSETEHSVLKR 130
           N KVIVLLD  +FP+LFD +KVEK+++  GT++PS   TGG R  ++LDPSE  H  LK+
Sbjct: 104 NPKVIVLLDGKSFPVLFDASKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEPNHEKLKK 163

Query: 131 HFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFDFIFRLLSDRS 190
                L+SR    IP F  + +E+F+ L+KE++      FN  SD A+F+F+ R L   +
Sbjct: 164 LMFFLLSSRRDHVIPEFHETYTELFETLDKEMEEKGTVGFNSGSDQAAFNFLARSLFGVN 223

Query: 191 P-DKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFALVKSAYRKLY 250
           P +    ++GP L+ +W+ +QL P+ TLGLPK     +D+++HTFRLP  LVK  Y++LY
Sbjct: 224 PVETKLGTDGPALIGKWILLQLHPVITLGLPKF---LDDVLLHTFRLPPILVKKDYQRLY 283

Query: 251 ESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWVGSAGEELHR 310
           + FY +S +   EAEK GI++++ACHNL+F   FN++ GMK+  P +L  +  AG E+H 
Sbjct: 284 DFFYTNSANLFIEAEKLGISKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGVEIHT 343

Query: 311 KLVGEIRAAVKIDGG-ITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDS 370
           +L  EIR+ VK  GG IT  A+EKM L+KSVVYE LR+DPPV  QY +AKQD+ IESHD+
Sbjct: 344 RLANEIRSEVKSAGGKITMSAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIESHDA 403

Query: 371 AFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNGRETEEPTA 430
            FE+KKGE++FGYQP ATKDPK+F+   EFV +RFVGE+GEKLLK+V WSNG ETE PT 
Sbjct: 404 VFEVKKGEILFGYQPFATKDPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETESPTV 463

Query: 431 ENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 479
            NKQC  +D VV++ R+ + E FLRY T  V+   S+LG S+ + SL KA
Sbjct: 464 GNKQCAGKDFVVMVSRLFVTEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

BLAST of Cucsat.G16467 vs. ExPASy Swiss-Prot
Match: P48417 (Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE=1 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 2.0e-136
Identity = 237/469 (50.53%), Postives = 329/469 (70.15%), Query Frame = 0

Query: 13  LPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRTNMPPGPFISSNS 72
           LP++ IPG YG P  GPI DR  YFY QGRE FF+SR+ KY STV+R NMPPGPFI+SN 
Sbjct: 70  LPIRQIPGDYGLPGIGPIQDRLDYFYNQGREEFFKSRLQKYKSTVYRANMPPGPFIASNP 129

Query: 73  KVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDPSETEHSVLKRHF 132
           +VIVLLDA +FP+LFD +KVEK+++  GTYMPS   TGG R  ++LDPSE  H+ LK+  
Sbjct: 130 RVIVLLDAKSFPVLFDMSKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPNHTKLKQLL 189

Query: 133 LKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFDFIFRLLSDRSP- 192
              + +R    IP F +S +++ + +E +L     A FN  ++ A+F+F+ R      P 
Sbjct: 190 FNLIKNRRDYVIPEFSSSFTDLCEVVEYDLATKGKAAFNDPAEQAAFNFLSRAFFGVKPI 249

Query: 193 DKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFALVKSAYRKLYES 252
           D     + P L+ +W+   LAP+ ++GLPK     E+  +H+ RLP  LV++ Y +LYE 
Sbjct: 250 DTPLGKDAPSLISKWVLFNLAPILSVGLPKE---VEEATLHSVRLPPLLVQNDYHRLYEF 309

Query: 253 FYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWVGSAGEELHRKL 312
           F  ++GS LDEAE+ GI+R++ACHN++F   FN++ G K+L P L+ W+G AG ELH KL
Sbjct: 310 FTSAAGSVLDEAEQSGISRDEACHNILFAVCFNSWGGFKILFPSLMKWIGRAGLELHTKL 369

Query: 313 VGEIRAAVKIDGG--ITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSA 372
             EIR+A++  GG  +T  A+E+M L+KSVVYE LRI+PPV  QY KAK+D ++ESH++A
Sbjct: 370 AQEIRSAIQSTGGGKVTMAAMEQMPLMKSVVYETLRIEPPVALQYGKAKKDFILESHEAA 429

Query: 373 FEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNGRETEEPTAE 432
           +++K+GEM+FGYQP ATKDPK+F+  EEFV +RFVGE G KL++YV WSNG ETE P+  
Sbjct: 430 YQVKEGEMLFGYQPFATKDPKIFDRPEEFVADRFVGE-GVKLMEYVMWSNGPETETPSVA 489

Query: 433 NKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 479
           NKQC  +D VV+  R+ +VELF RY++F +E   SSLG S+ + SL ++
Sbjct: 490 NKQCAGKDFVVMAARLFVVELFKRYDSFDIEVGTSSLGASITLTSLKRS 534

BLAST of Cucsat.G16467 vs. NCBI nr
Match: XP_004137005.1 (allene oxide synthase 3 [Cucumis sativus] >KGN43980.1 hypothetical protein Csa_017715 [Cucumis sativus])

HSP 1 Score: 959 bits (2478), Expect = 0.0
Identity = 477/478 (99.79%), Postives = 478/478 (100.00%), Query Frame = 0

Query: 1   MTSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRT 60
           MTSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRT
Sbjct: 1   MTSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRT 60

Query: 61  NMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDP 120
           NMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDP
Sbjct: 61  NMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDP 120

Query: 121 SETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFD 180
           SETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFD
Sbjct: 121 SETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFD 180

Query: 181 FIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFAL 240
           FIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFAL
Sbjct: 181 FIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFAL 240

Query: 241 VKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWV 300
           VKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWV
Sbjct: 241 VKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWV 300

Query: 301 GSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQD 360
           GSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQD
Sbjct: 301 GSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQD 360

Query: 361 IVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNG 420
           IVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNG
Sbjct: 361 IVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNG 420

Query: 421 RETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 478
           RETEEPTAENKQCPARDLVVLMCRVVLVELFLRY+TFTVEGTRSSLGWSVKVKSLTKA
Sbjct: 421 RETEEPTAENKQCPARDLVVLMCRVVLVELFLRYDTFTVEGTRSSLGWSVKVKSLTKA 478

BLAST of Cucsat.G16467 vs. NCBI nr
Match: XP_008454884.1 (PREDICTED: allene oxide synthase 3-like [Cucumis melo] >KAA0031258.1 allene oxide synthase 3-like [Cucumis melo var. makuwa] >TYK06709.1 allene oxide synthase 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 454/479 (94.78%), Postives = 466/479 (97.29%), Query Frame = 0

Query: 1   MTSSSS-EHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFR 60
           MTSSSS EHP IPLPLKPIPG+ GFPIFGPI DRYHYFYIQGRETFFRSRMAKYNSTVFR
Sbjct: 1   MTSSSSPEHPHIPLPLKPIPGTNGFPIFGPIKDRYHYFYIQGRETFFRSRMAKYNSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120
           TNMPPGPFISSNSKVIVLLDAL+FPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASF 180
           PSETEHSVLKRHFL FLASRHHQFIPLFRTSISEMFDKLEKEL+NNNVANFNP+SD ASF
Sbjct: 121 PSETEHSVLKRHFLNFLASRHHQFIPLFRTSISEMFDKLEKELKNNNVANFNPVSDSASF 180

Query: 181 DFIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240
           DFIFRLLSDRSP+KNF SEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA
Sbjct: 181 DFIFRLLSDRSPNKNFISEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNW 300
           LVKSAYRKLYESFYESSG+FLDEAEKQGINREKACHNLVFLAGFNAYAGMKVL PILLNW
Sbjct: 241 LVKSAYRKLYESFYESSGTFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLFPILLNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAGEELHRKLV EIRAAVK D G+TFGALEKMSLLKSVVYEVLRIDPPVPYQYA+AKQ
Sbjct: 301 VGSAGEELHRKLVSEIRAAVKKDEGVTFGALEKMSLLKSVVYEVLRIDPPVPYQYARAKQ 360

Query: 361 DIVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSN 420
           DIVIE+HDSAF+IKKGEMIFGYQPIATKDPKVFENAEEFVG+RFVGEKGEKLLKYVYWSN
Sbjct: 361 DIVIENHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 478
           GRETEEPTAENKQCPARDLVVLM RVVLVELFLRY+TF VEGTRSSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 479

BLAST of Cucsat.G16467 vs. NCBI nr
Match: XP_038888826.1 (allene oxide synthase 3-like [Benincasa hispida])

HSP 1 Score: 863 bits (2231), Expect = 1.44e-314
Identity = 426/479 (88.94%), Postives = 455/479 (94.99%), Query Frame = 0

Query: 1   MTSS-SSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFR 60
           MTSS SSE P IPLPLKPIPGSYGFPIFGPI DRY YFYIQGRE FFR+RMAKY+STVFR
Sbjct: 1   MTSSPSSEQPLIPLPLKPIPGSYGFPIFGPIKDRYDYFYIQGREKFFRTRMAKYDSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120
           TNMPPGPFISSNSKVIVLLDAL+FPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASF 180
           PSETEHS+LKRHFLKFLASRHHQFIPLFR+S+SEMF+KL++EL+ N VA+FNP+SD ASF
Sbjct: 121 PSETEHSLLKRHFLKFLASRHHQFIPLFRSSVSEMFNKLDRELEKNKVADFNPVSDSASF 180

Query: 181 DFIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240
           DF+FRLLSDRSPDK+ SS+GPGLVDRWL +QLAPLATLGLPK  SCFED+IIHTFRLPFA
Sbjct: 181 DFVFRLLSDRSPDKSLSSKGPGLVDRWLALQLAPLATLGLPKFLSCFEDIIIHTFRLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNW 300
           L +SAY KLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNW
Sbjct: 241 LARSAYSKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAGE LHRKL  EIRAAVK DGG+TF ALEKMSLLKSV+YEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGEGLHRKLAVEIRAAVKEDGGVTFSALEKMSLLKSVIYEVLRIDPPVPYQYAKAKQ 360

Query: 361 DIVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSN 420
           DIVIESHDSAF+IKKGEMIFGYQPIATKDP+VFEN EEF+G+RFVGEKGEKLLKYVYWSN
Sbjct: 361 DIVIESHDSAFKIKKGEMIFGYQPIATKDPRVFENPEEFIGDRFVGEKGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 478
           GRETEEPTAENKQC ARDLVVL+ RVVLVELFLRY+TF VEGT+SSLGWSVKVKSL+KA
Sbjct: 421 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTQSSLGWSVKVKSLSKA 479

BLAST of Cucsat.G16467 vs. NCBI nr
Match: KAG6572010.1 (Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 821 bits (2120), Expect = 1.08e-297
Identity = 403/477 (84.49%), Postives = 436/477 (91.40%), Query Frame = 0

Query: 2   TSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRTN 61
           +SSSSEHP  PLPLKPIPGSYG P FGPI  RY YFY QGRE FFRSRMA+Y STVFRTN
Sbjct: 3   SSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFRTN 62

Query: 62  MPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDPS 121
           MPPGPFISS+S+VIVLLDA +FPILFDTTKVEKRNVLDGTYMPS++FTGGIRTCAFLDPS
Sbjct: 63  MPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLDPS 122

Query: 122 ETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFDF 181
           ETEHSVLKRHFLKFLASRH +FIPLFR S+SEMF KLEKEL+ NN A+FN +SD ASFDF
Sbjct: 123 ETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNNAADFNSVSDAASFDF 182

Query: 182 IFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFALV 241
           IFRLLSD  PD+   S+G  LVDRWL MQLAPLATLGLPK  SCFEDLIIHTF LPFA V
Sbjct: 183 IFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFAFV 242

Query: 242 KSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWVG 301
           K+AYRKLY++FY+SS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNWVG
Sbjct: 243 KNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNWVG 302

Query: 302 SAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 361
           SAG+ELHRKL GEIRAAVK + G+TF A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQDI
Sbjct: 303 SAGKELHRKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 362

Query: 362 VIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNGR 421
           +IESHDSAFEIKKGEMIFGYQPIAT+DPKVFE  EEF+G+RFVGE+GEKLLKYVYWSNGR
Sbjct: 363 IIESHDSAFEIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWSNGR 422

Query: 422 ETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 478
           ETEEPTAENKQCPARDLVVL+ RVVL ELFLRY+TF VEGT+SSLGWSVKVKSLTKA
Sbjct: 423 ETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTKA 479

BLAST of Cucsat.G16467 vs. NCBI nr
Match: XP_022952977.1 (allene oxide synthase 3-like [Cucurbita moschata])

HSP 1 Score: 818 bits (2113), Expect = 1.26e-296
Identity = 401/476 (84.24%), Postives = 436/476 (91.60%), Query Frame = 0

Query: 2   TSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRTN 61
           +SSSSEHP  PLPLKPIPGSYG P FGPI  RY YFY QGRE FFRSRMA+Y STVFRTN
Sbjct: 3   SSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFRTN 62

Query: 62  MPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDPS 121
           MPPGPFISS+S+VIVLLDA +FPILFDTTKVEKRNVLDGTYMPS++FTGGIRTCAFLDPS
Sbjct: 63  MPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLDPS 122

Query: 122 ETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFDF 181
           ETEHSVLKRHFLKFLASRH +FIPLFR S+SEMF KLEKEL+ NN A+FN +SD ASFDF
Sbjct: 123 ETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNNAADFNSVSDAASFDF 182

Query: 182 IFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFALV 241
           IFRLLSD  PD+   S+G  LVDRWL MQLAPLATLGLPK  SCFEDLIIHTF LPFALV
Sbjct: 183 IFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFALV 242

Query: 242 KSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWVG 301
           K+AYRKLY++FY+SS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNWVG
Sbjct: 243 KNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNWVG 302

Query: 302 SAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 361
           SAG+ELH+KL GEIRAAVK + G+TF A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQDI
Sbjct: 303 SAGKELHKKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 362

Query: 362 VIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNGR 421
           +IESHDSAF+IKKGEMIFGYQPIAT+DPKVFE  EEF+G+RFVGE+GEKLLKYVYWSNGR
Sbjct: 363 IIESHDSAFKIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWSNGR 422

Query: 422 ETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTK 477
           ETEEPTAENKQCPARDLVVL+ RVVL ELFLRY+TF VEGT+SSLGWSVKVKSLTK
Sbjct: 423 ETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTK 478

BLAST of Cucsat.G16467 vs. ExPASy TrEMBL
Match: A0A0A0K2U8 (Fatty acid 9-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G075600 PE=4 SV=1)

HSP 1 Score: 959 bits (2478), Expect = 0.0
Identity = 477/478 (99.79%), Postives = 478/478 (100.00%), Query Frame = 0

Query: 1   MTSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRT 60
           MTSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRT
Sbjct: 1   MTSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRT 60

Query: 61  NMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDP 120
           NMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDP
Sbjct: 61  NMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDP 120

Query: 121 SETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFD 180
           SETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFD
Sbjct: 121 SETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFD 180

Query: 181 FIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFAL 240
           FIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFAL
Sbjct: 181 FIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFAL 240

Query: 241 VKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWV 300
           VKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWV
Sbjct: 241 VKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWV 300

Query: 301 GSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQD 360
           GSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQD
Sbjct: 301 GSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQD 360

Query: 361 IVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNG 420
           IVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNG
Sbjct: 361 IVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNG 420

Query: 421 RETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 478
           RETEEPTAENKQCPARDLVVLMCRVVLVELFLRY+TFTVEGTRSSLGWSVKVKSLTKA
Sbjct: 421 RETEEPTAENKQCPARDLVVLMCRVVLVELFLRYDTFTVEGTRSSLGWSVKVKSLTKA 478

BLAST of Cucsat.G16467 vs. ExPASy TrEMBL
Match: A0A5A7SKU9 (Allene oxide synthase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00100 PE=4 SV=1)

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 454/479 (94.78%), Postives = 466/479 (97.29%), Query Frame = 0

Query: 1   MTSSSS-EHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFR 60
           MTSSSS EHP IPLPLKPIPG+ GFPIFGPI DRYHYFYIQGRETFFRSRMAKYNSTVFR
Sbjct: 1   MTSSSSPEHPHIPLPLKPIPGTNGFPIFGPIKDRYHYFYIQGRETFFRSRMAKYNSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120
           TNMPPGPFISSNSKVIVLLDAL+FPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASF 180
           PSETEHSVLKRHFL FLASRHHQFIPLFRTSISEMFDKLEKEL+NNNVANFNP+SD ASF
Sbjct: 121 PSETEHSVLKRHFLNFLASRHHQFIPLFRTSISEMFDKLEKELKNNNVANFNPVSDSASF 180

Query: 181 DFIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240
           DFIFRLLSDRSP+KNF SEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA
Sbjct: 181 DFIFRLLSDRSPNKNFISEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNW 300
           LVKSAYRKLYESFYESSG+FLDEAEKQGINREKACHNLVFLAGFNAYAGMKVL PILLNW
Sbjct: 241 LVKSAYRKLYESFYESSGTFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLFPILLNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAGEELHRKLV EIRAAVK D G+TFGALEKMSLLKSVVYEVLRIDPPVPYQYA+AKQ
Sbjct: 301 VGSAGEELHRKLVSEIRAAVKKDEGVTFGALEKMSLLKSVVYEVLRIDPPVPYQYARAKQ 360

Query: 361 DIVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSN 420
           DIVIE+HDSAF+IKKGEMIFGYQPIATKDPKVFENAEEFVG+RFVGEKGEKLLKYVYWSN
Sbjct: 361 DIVIENHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 478
           GRETEEPTAENKQCPARDLVVLM RVVLVELFLRY+TF VEGTRSSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 479

BLAST of Cucsat.G16467 vs. ExPASy TrEMBL
Match: A0A1S3BZQ0 (allene oxide synthase 3-like OS=Cucumis melo OX=3656 GN=LOC103495194 PE=4 SV=1)

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 454/479 (94.78%), Postives = 466/479 (97.29%), Query Frame = 0

Query: 1   MTSSSS-EHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFR 60
           MTSSSS EHP IPLPLKPIPG+ GFPIFGPI DRYHYFYIQGRETFFRSRMAKYNSTVFR
Sbjct: 1   MTSSSSPEHPHIPLPLKPIPGTNGFPIFGPIKDRYHYFYIQGRETFFRSRMAKYNSTVFR 60

Query: 61  TNMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120
           TNMPPGPFISSNSKVIVLLDAL+FPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASF 180
           PSETEHSVLKRHFL FLASRHHQFIPLFRTSISEMFDKLEKEL+NNNVANFNP+SD ASF
Sbjct: 121 PSETEHSVLKRHFLNFLASRHHQFIPLFRTSISEMFDKLEKELKNNNVANFNPVSDSASF 180

Query: 181 DFIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240
           DFIFRLLSDRSP+KNF SEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA
Sbjct: 181 DFIFRLLSDRSPNKNFISEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240

Query: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNW 300
           LVKSAYRKLYESFYESSG+FLDEAEKQGINREKACHNLVFLAGFNAYAGMKVL PILLNW
Sbjct: 241 LVKSAYRKLYESFYESSGTFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLFPILLNW 300

Query: 301 VGSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360
           VGSAGEELHRKLV EIRAAVK D G+TFGALEKMSLLKSVVYEVLRIDPPVPYQYA+AKQ
Sbjct: 301 VGSAGEELHRKLVSEIRAAVKKDEGVTFGALEKMSLLKSVVYEVLRIDPPVPYQYARAKQ 360

Query: 361 DIVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSN 420
           DIVIE+HDSAF+IKKGEMIFGYQPIATKDPKVFENAEEFVG+RFVGEKGEKLLKYVYWSN
Sbjct: 361 DIVIENHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420

Query: 421 GRETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 478
           GRETEEPTAENKQCPARDLVVLM RVVLVELFLRY+TF VEGTRSSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 479

BLAST of Cucsat.G16467 vs. ExPASy TrEMBL
Match: A0A6J1GLX0 (allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111455491 PE=4 SV=1)

HSP 1 Score: 818 bits (2113), Expect = 6.09e-297
Identity = 401/476 (84.24%), Postives = 436/476 (91.60%), Query Frame = 0

Query: 2   TSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRTN 61
           +SSSSEHP  PLPLKPIPGSYG P FGPI  RY YFY QGRE FFRSRMA+Y STVFRTN
Sbjct: 3   SSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFRTN 62

Query: 62  MPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDPS 121
           MPPGPFISS+S+VIVLLDA +FPILFDTTKVEKRNVLDGTYMPS++FTGGIRTCAFLDPS
Sbjct: 63  MPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLDPS 122

Query: 122 ETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFDF 181
           ETEHSVLKRHFLKFLASRH +FIPLFR S+SEMF KLEKEL+ NN A+FN +SD ASFDF
Sbjct: 123 ETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNNAADFNSVSDAASFDF 182

Query: 182 IFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFALV 241
           IFRLLSD  PD+   S+G  LVDRWL MQLAPLATLGLPK  SCFEDLIIHTF LPFALV
Sbjct: 183 IFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFALV 242

Query: 242 KSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWVG 301
           K+AYRKLY++FY+SS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNWVG
Sbjct: 243 KNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNWVG 302

Query: 302 SAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 361
           SAG+ELH+KL GEIRAAVK + G+TF A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQDI
Sbjct: 303 SAGKELHKKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 362

Query: 362 VIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNGR 421
           +IESHDSAF+IKKGEMIFGYQPIAT+DPKVFE  EEF+G+RFVGE+GEKLLKYVYWSNGR
Sbjct: 363 IIESHDSAFKIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWSNGR 422

Query: 422 ETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTK 477
           ETEEPTAENKQCPARDLVVL+ RVVL ELFLRY+TF VEGT+SSLGWSVKVKSLTK
Sbjct: 423 ETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTK 478

BLAST of Cucsat.G16467 vs. ExPASy TrEMBL
Match: A0A6J1IB84 (allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470925 PE=4 SV=1)

HSP 1 Score: 815 bits (2106), Expect = 7.10e-296
Identity = 403/477 (84.49%), Postives = 433/477 (90.78%), Query Frame = 0

Query: 2   TSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRTN 61
           +SSSSEHP  P  LKPIPG YG P FGPI  RY YFY QGRE FFRSRMA+Y STVFRTN
Sbjct: 3   SSSSSEHPPAPPSLKPIPGGYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFRTN 62

Query: 62  MPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDPS 121
           MPPGPFISS+S+VIVLLDA +FPILFDTTKVEKRNVLDGTYMPS++FTGGIRTCAFLDPS
Sbjct: 63  MPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLDPS 122

Query: 122 ETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASFDF 181
           ETEHSVLKRHFLKFLASRH +FIPLFRTS+SEMF KLEKEL+  N A+FN +SD ASFDF
Sbjct: 123 ETEHSVLKRHFLKFLASRHDKFIPLFRTSVSEMFSKLEKELEKTNAADFNSVSDAASFDF 182

Query: 182 IFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFALV 241
           IFRLLSD  P +   S G  LVDRWL MQLAPLATLGLPK  SCFEDLIIHTF LPFALV
Sbjct: 183 IFRLLSDGKPGEKLGSNGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFALV 242

Query: 242 KSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNWVG 301
           K+AYRKLY++FYESS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNWVG
Sbjct: 243 KNAYRKLYDAFYESSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNWVG 302

Query: 302 SAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 361
           SAG+ELHRKL GEIRAAVK + G+TF A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQDI
Sbjct: 303 SAGKELHRKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQDI 362

Query: 362 VIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSNGR 421
           +IESHDSAFEIKKGEMIFGYQPIAT+DPKVFE  EEF+GERFVGE+GEKLLKYVYWSNGR
Sbjct: 363 IIESHDSAFEIKKGEMIFGYQPIATRDPKVFEKPEEFIGERFVGEEGEKLLKYVYWSNGR 422

Query: 422 ETEEPTAENKQCPARDLVVLMCRVVLVELFLRYETFTVEGTRSSLGWSVKVKSLTKA 478
           ETEEPTAENKQCPARDLVVL+ RVVL ELFLRY+TFTVEGT+SSLGWSVKVKSLTKA
Sbjct: 423 ETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFTVEGTQSSLGWSVKVKSLTKA 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GZP51.1e-15355.42Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1[more]
K4BV524.4e-14753.75Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P... [more]
Q962429.4e-14252.78Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P... [more]
Q9LLB02.1e-14152.55Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 P... [more]
P484172.0e-13650.53Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE... [more]
Match NameE-valueIdentityDescription
XP_004137005.10.099.79allene oxide synthase 3 [Cucumis sativus] >KGN43980.1 hypothetical protein Csa_0... [more]
XP_008454884.10.094.78PREDICTED: allene oxide synthase 3-like [Cucumis melo] >KAA0031258.1 allene oxid... [more]
XP_038888826.11.44e-31488.94allene oxide synthase 3-like [Benincasa hispida][more]
KAG6572010.11.08e-29784.49Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022952977.11.26e-29684.24allene oxide synthase 3-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0K2U80.099.79Fatty acid 9-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G075600 PE=4... [more]
A0A5A7SKU90.094.78Allene oxide synthase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BZQ00.094.78allene oxide synthase 3-like OS=Cucumis melo OX=3656 GN=LOC103495194 PE=4 SV=1[more]
A0A6J1GLX06.09e-29784.24allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111455491 PE=4 ... [more]
A0A6J1IB847.10e-29684.49allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470925 PE=4 SV... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 386..404
score: 24.7
coord: 333..349
score: 37.1
coord: 370..384
score: 17.18
coord: 433..451
score: 17.21
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 13..478
e-value: 3.1E-112
score: 377.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 12..462
IPR001128Cytochrome P450PFAMPF00067p450coord: 306..416
e-value: 1.4E-15
score: 57.0
NoneNo IPR availablePANTHERPTHR24286:SF302HYDROPEROXIDE LYASEcoord: 13..459
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 13..459

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G16467.T1Cucsat.G16467.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen