Cucsat.G16214 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G16214
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Serine proteinase inhibitor
Location	ctg2231: 182323 .. 183285 (+)
RNA-Seq Expression	Cucsat.G16214
Synteny	Cucsat.G16214

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGAATTTTCTAAACTCATCCATAACATTTTTCCCTATAAATACTCACAACTCTTCCCTATTTGCATTATCCAAACACTATTATTCTTCAACAAATACATTCAAACCCAAATTCACATTCCTCAACTTCAACCAAACGATGGCTGATATTTGTCGTCCTGGTATGTTTCCATTTATGGTTTTACATTACTACAAGTTTTGAGACACTCTGTATACATTTTATAAAGTGTGCTCCTGAGTTTTCTATTTCAATGTTTACAATTGGGGTTAGATTTTTTGTAATCGAATATACCCATTTGAAGCATCTTTTTGCATAGAATGTGTTTCTGAGTTTTGCTTCTTCATTATAATTATGAGATACTTCTGTTGATGATCTTTAGACTGTAGTTTTGTTTGGATTATCTCTTTGGATTTTTCATATGGTCGTGATTATTTGATTATTTGTTTTGGTATATAATTATGATTAGAGAAAAACCAGTGGCCAGAACTTGTTGGAATAAAAGCAACAATTGCGGAGTATATCATCAAGAAAGACAATCCTAATGTAGAAAATGTTATAGTTCTCTTGGCTGGAACTGGAACCACTAAAGATTTTAGGAGTGATCGAGTTTGGTTGTTTACTAATATAGACGGGAGAGTTGTTGAAATGCCAACGGTGGGTTAGATAAAAGACTATCAAAATACGTTGTATGCTTTTATTTGTATTCCTAAATCACTTTCTAAAACTATTGATGTAAGCAAAATAAGGGTAGAAACTATACCATATATATTAATATTTCTACCACATGTTTAATTATCAATTTCCCTTTGTCTTATCTATATTGTATTATTTCTAGTTTTTCTTTTTCATATCAATTTGTAAAAATAAATCAAAGTGGAAAAGTCATAAAAATCATGAGAAACTCTTCTGTATACATTATGATTTCATTTTCAGAAGTAAACAACGAAAAAGTGCTATATGTGT

Coding sequence (CDS)

CTGAATTTTCTAAACTCATCCATAACATTTTTCCCTATAAATACTCACAACTCTTCCCTATTTGCATTATCCAAACACTATTATTCTTCAACAAATACATTCAAACCCAAATTCACATTCCTCAACTTCAACCAAACGATGGCTGATATTTGTCGTCCTGAGAAAAACCAGTGGCCAGAACTTGTTGGAATAAAAGCAACAATTGCGGAGTATATCATCAAGAAAGACAATCCTAATGTAGAAAATGTTATAGTTCTCTTGGCTGGAACTGGAACCACTAAAGATTTTAGGAGTGATCGAGTTTGGTTGTTTACTAATATAGACGGGAGAGTTGTTGAAATGCCAACGGTGGGTTAG

Protein sequence

LNFLNSSITFFPINTHNSSLFALSKHYYSSTNTFKPKFTFLNFNQTMADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTNIDGRVVEMPTVG

Homology

[Close]

BLAST of Cucsat.G16214 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.0e-12
Identity = 34/63 (53.97%), Postives = 46/63 (73.02%), Query Frame = 0


Query: 55  KNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTNIDGRVVEM 114
           K  WPEL+G+ A  A+ II+K+NP++ N+ +LL G+  TKDFR DRV LF NI G VV++
Sbjct: 48  KQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQI 107

Query: 115 PTV 118
           P V
Sbjct: 108 PRV 110

[Close]

BLAST of Cucsat.G16214 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.1e-11
Identity = 31/63 (49.21%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 55  KNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTNIDGRVVEM 114
           K  WPEL+G+   +A+ II+K+NP++ N+ +LL+G+  T D+  DRV LF NI G VV+M
Sbjct: 48  KQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQM 107

Query: 115 PTV 118
           P V
Sbjct: 108 PVV 110

[Close]

BLAST of Cucsat.G16214 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 9.5e-11
Identity = 32/64 (50.00%), Postives = 44/64 (68.75%), Query Frame = 0


Query: 55  KNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTNIDGRVVEM 114
           K +WPEL+G+   +A+ II+K+N  + NV +LL G+  T D R DRV LF NI G VV++
Sbjct: 44  KLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYVVDI 103

Query: 115 PTVG 119
           P VG
Sbjct: 104 PVVG 107

[Close]

BLAST of Cucsat.G16214 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 2.8e-10
Identity = 31/65 (47.69%), Postives = 43/65 (66.15%), Query Frame = 0


Query: 54  EKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTNIDGRVVE 113
           +K  WPEL+G+ A  A  II+K+N  + NV  +L G+  TKDFR +RV LF N+   VV+
Sbjct: 30  KKETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQ 89

Query: 114 MPTVG 119
           +P VG
Sbjct: 90  IPRVG 94

[Close]

BLAST of Cucsat.G16214 vs. ExPASy Swiss-Prot
Match: P01052 (Chymotrypsin inhibitor I, A, B and C subunits OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 4.7e-10
Identity = 31/63 (49.21%), Postives = 43/63 (68.25%), Query Frame = 0


Query: 55  KNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTNIDGRVVEM 114
           K QWPEL+G+   +A+ II+K N  + NV +LL G+  T DFR +RV LF +I G VV++
Sbjct: 8   KLQWPELIGVPTKLAKEIIEKQNSLISNVHILLNGSPVTMDFRCNRVRLFDDILGSVVQI 67

Query: 115 PTV 118
           P V
Sbjct: 68  PRV 70

[Close]

BLAST of Cucsat.G16214 vs. NCBI nr
Match: KGN50838.1 (hypothetical protein Csa_004692 [Cucumis sativus])

HSP 1 Score: 151 bits (381), Expect = 3.02e-45
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 0


Query: 47  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 106
           MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN
Sbjct: 1   MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 60

Query: 107 IDGRVVEMPTVG 118
           IDGRVVEMPTVG
Sbjct: 61  IDGRVVEMPTVG 72

[Close]

BLAST of Cucsat.G16214 vs. NCBI nr
Match: ALD47589.1 (serine proteinase inhibitor [Cucumis metulifer])

HSP 1 Score: 122 bits (307), Expect = 5.72e-34
Identity = 58/72 (80.56%), Postives = 63/72 (87.50%), Query Frame = 0


Query: 47  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 106
           MADIC P KNQWPELVGIKAT AEYII+KDNP+VE VIVLLAGTGTTKD R DRVWLF N
Sbjct: 1   MADICPPGKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVN 60

Query: 107 IDGRVVEMPTVG 118
           I+ +VVE+P VG
Sbjct: 61  IEEKVVEIPKVG 72

[Close]

BLAST of Cucsat.G16214 vs. NCBI nr
Match: KGN50840.1 (hypothetical protein Csa_004718 [Cucumis sativus])

HSP 1 Score: 119 bits (298), Expect = 1.34e-32
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = 0


Query: 47  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 106
           MADIC P KNQWPELVG+KAT A+YIIKKDNPNVENV+VLLAG+GTT+D R DRVW+F N
Sbjct: 1   MADICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVN 60

Query: 107 IDGRVVEMPTVG 118
           I   VVE+P VG
Sbjct: 61  IHDLVVEVPKVG 72

[Close]

BLAST of Cucsat.G16214 vs. NCBI nr
Match: KAG6571494.1 (hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 99.8 bits (247), Expect = 7.72e-25
Identity = 45/72 (62.50%), Postives = 58/72 (80.56%), Query Frame = 0


Query: 47  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 106
           MAD C P KN+WPELVGIK+TIAE+II+KDN NVE+V  +LAG+  T+D R DRV +F N
Sbjct: 1   MADFCPPGKNRWPELVGIKSTIAEHIIRKDNSNVEHVETILAGSAVTEDLRCDRVRIFVN 60

Query: 107 IDGRVVEMPTVG 118
           I G +V++PT+G
Sbjct: 61  IKGIIVQIPTIG 72

[Close]

BLAST of Cucsat.G16214 vs. NCBI nr
Match: XP_026426365.1 (inhibitor of trypsin and hageman factor-like [Papaver somniferum] >RZC83600.1 hypothetical protein C5167_046386 [Papaver somniferum])

HSP 1 Score: 83.6 bits (205), Expect = 1.80e-18
Identity = 42/72 (58.33%), Postives = 49/72 (68.06%), Query Frame = 0


Query: 47  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 106
           MA  C P K+ WPELVG     A  II+++ PNV N IVLL GT TT DFR DRVW+F N
Sbjct: 1   MASECGPGKSSWPELVGFDGETAARIIEREIPNV-NAIVLLEGTPTTLDFRCDRVWVFVN 60

Query: 107 IDGRVVEMPTVG 118
             G+VVE PT+G
Sbjct: 61  EAGKVVETPTIG 71

[Close]

BLAST of Cucsat.G16214 vs. ExPASy TrEMBL
Match: A0A0A0KMM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1)

HSP 1 Score: 151 bits (381), Expect = 1.46e-45
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 0


Query: 47  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 106
           MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN
Sbjct: 1   MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 60

Query: 107 IDGRVVEMPTVG 118
           IDGRVVEMPTVG
Sbjct: 61  IDGRVVEMPTVG 72

[Close]

BLAST of Cucsat.G16214 vs. ExPASy TrEMBL
Match: A0A0M3SAG8 (Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1)

HSP 1 Score: 122 bits (307), Expect = 2.77e-34
Identity = 58/72 (80.56%), Postives = 63/72 (87.50%), Query Frame = 0


Query: 47  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 106
           MADIC P KNQWPELVGIKAT AEYII+KDNP+VE VIVLLAGTGTTKD R DRVWLF N
Sbjct: 1   MADICPPGKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVN 60

Query: 107 IDGRVVEMPTVG 118
           I+ +VVE+P VG
Sbjct: 61  IEEKVVEIPKVG 72

[Close]

BLAST of Cucsat.G16214 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 119 bits (298), Expect = 6.49e-33
Identity = 55/72 (76.39%), Postives = 62/72 (86.11%), Query Frame = 0


Query: 47  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 106
           MADIC P KNQWPELVG+KAT A+YIIKKDNPNVENV+VLLAG+GTT+D R DRVW+F N
Sbjct: 1   MADICPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVN 60

Query: 107 IDGRVVEMPTVG 118
           I   VVE+P VG
Sbjct: 61  IHDLVVEVPKVG 72

[Close]

BLAST of Cucsat.G16214 vs. ExPASy TrEMBL
Match: A0A4Y7LG33 (Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_046386 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 8.72e-19
Identity = 42/72 (58.33%), Postives = 49/72 (68.06%), Query Frame = 0


Query: 47  MADICRPEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTN 106
           MA  C P K+ WPELVG     A  II+++ PNV N IVLL GT TT DFR DRVW+F N
Sbjct: 1   MASECGPGKSSWPELVGFDGETAARIIEREIPNV-NAIVLLEGTPTTLDFRCDRVWVFVN 60

Query: 107 IDGRVVEMPTVG 118
             G+VVE PT+G
Sbjct: 61  EAGKVVETPTIG 71

[Close]

BLAST of Cucsat.G16214 vs. ExPASy TrEMBL
Match: A0A4Y7LFX7 (Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_046390 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.50e-17
Identity = 39/66 (59.09%), Postives = 46/66 (69.70%), Query Frame = 0


Query: 53  PEKNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLFTNIDGRVV 112
           P K+ WPELVG     A  II++ NPNV N IVLL GT TT DFR DRVW+F N  G+VV
Sbjct: 15  PGKSSWPELVGFNGGTAARIIERQNPNV-NAIVLLEGTPTTLDFRCDRVWVFVNEAGKVV 74

Query: 113 EMPTVG 118
           + PT+G
Sbjct: 75  QTPTIG 79

The following BLAST results are available for this feature:

BLAST of Cucsat.G16214 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P16231	1.0e-12	53.97	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P05118	1.1e-11	49.21	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]
Q00783	9.5e-11	50.00	Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1	[more]
Q02214	2.8e-10	47.69	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P01052	4.7e-10	49.21	Chymotrypsin inhibitor I, A, B and C subunits OS=Solanum tuberosum OX=4113 PE=1 ...	[more]

BLAST of Cucsat.G16214 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN50838.1	3.02e-45	100.00	hypothetical protein Csa_004692 [Cucumis sativus]	[more]
ALD47589.1	5.72e-34	80.56	serine proteinase inhibitor [Cucumis metulifer]	[more]
KGN50840.1	1.34e-32	76.39	hypothetical protein Csa_004718 [Cucumis sativus]	[more]
KAG6571494.1	7.72e-25	62.50	hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_026426365.1	1.80e-18	58.33	inhibitor of trypsin and hageman factor-like [Papaver somniferum] >RZC83600.1 hy...	[more]

BLAST of Cucsat.G16214 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KMM0	1.46e-45	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1	[more]
A0A0M3SAG8	2.77e-34	80.56	Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1	[more]
A0A0A0KME7	6.49e-33	76.39	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1	[more]
A0A4Y7LG33	8.72e-19	58.33	Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_046386 PE=3 SV=1	[more]
A0A4Y7LFX7	2.50e-17	59.09	Uncharacterized protein OS=Papaver somniferum OX=3469 GN=C5167_046390 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 57..70 score: 47.52 coord: 83..94 score: 33.33 coord: 95..105 score: 49.8
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 55..118 e-value: 2.8E-22 score: 79.2
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 41..118
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 58..69
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 51..118 e-value: 1.0E-21 score: 79.0
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 41..118
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 52..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G16214.T1	Cucsat.G16214.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity