Cucsat.G16028 (gene) Cucumber (B10) v3

BLAST of Cucsat.G16028 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.4e-74
Identity = 153/374 (40.91%), Postives = 221/374 (59.09%), Query Frame = 0

Query: 29  LGICFFFFFILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNYLNGTIRT 88
           + I F  + I+  I +         + N  L   +   F+FG+S  DAGNNNY++     
Sbjct: 7   VSITFLAYTIIISIGSINC------IDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSV 66

Query: 89  RSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDP--NNSNYMNGVNFGSG 148
           RSN+ PYG++ F  PTGR  DGR+IPDF+AEYA +P +PP L P   NS +  GVNF SG
Sbjct: 67  RSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASG 126

Query: 149 GAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGDILH 208
           GA  L  + +   ++L+TQ+  FK VE+ +R  +G +   +  +S +V+LF+IG  D  +
Sbjct: 127 GAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLG-DAEGKRVISRAVYLFHIGLNDYQY 186

Query: 209 PFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRL--- 268
           PF ++  +F +I S E+Y + V+ NMT   KE+YNLGGRKFG+L   P    P+S +   
Sbjct: 187 PFTTNSSLFQSI-SNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQ 246

Query: 269 AKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKV 328
            K     Q    L  ++N+ LL  L++L  +L GFKY+  D +    +R+ +P+KYGFK 
Sbjct: 247 TKIRSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKE 306

Query: 329 VDTACCGSDEFRGSYNCGRNTG-TIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGG 388
              ACCGS   RG   CG   G +  +  C+N++DYLF+D +H TEKA  Q A+LIWSG 
Sbjct: 307 GKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGP 366

Query: 389 VDIVKPYSFKQLFQ 397
            +I  PY+ K LF+
Sbjct: 367 TNITGPYNLKALFE 372

BLAST of Cucsat.G16028 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 273.9 bits (699), Expect = 3.0e-72
Identity = 141/339 (41.59%), Postives = 217/339 (64.01%), Query Frame = 0

Query: 67  FIFGNSFVDAGNNNYLNGTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFL 126
           F+FG+SF+DAGNNNY+N T   ++NFPPYG++FF +PTGR+ DGR+I DF+AEYA +P +
Sbjct: 50  FLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLI 109

Query: 127 PPFLDPNNS-NYMNGVNFGSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETL 186
           PPFL+P NS   + GVNF S GA  L E+   + ++L+TQ++ +K VE+  R + G E  
Sbjct: 110 PPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEE- 169

Query: 187 SQTFLSNSVFLFNIGGGDILHPFESSFDIFNTIE-SQEQYANMVINNMTIALKEIYNLGG 246
           S+  +S +V+L +IG  D    + S F    ++  S  Q+ ++VI N+T  + EIY +GG
Sbjct: 170 SKKRISRAVYLISIGSND----YSSIFLTNQSLPISMSQHVDIVIGNLTTFIHEIYKIGG 229

Query: 247 RKFGVLGVLPSGYLPSSRLAK---NEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYS 306
           RKFG L V   G  P+ R+ +   ++  ++ ++ L+ ++N+ L   L ++ +Q+KGFK+S
Sbjct: 230 RKFGFLNVPDLGCFPALRILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFS 289

Query: 307 YVDAYNFFMQRIQNPTKYGFKVVDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFY 366
             D       R+Q+P+K+GFK  + ACCG+ ++RG ++CG       +  C+N  DY+F+
Sbjct: 290 LFDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFW 349

Query: 367 DSYHPTEKAYEQFAKLIWSGG----VDIVKPYSFKQLFQ 397
           DS H T+  Y QFA LIW+GG      +V PY+   LFQ
Sbjct: 350 DSLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQ 383

BLAST of Cucsat.G16028 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.1e-71
Identity = 145/351 (41.31%), Postives = 214/351 (60.97%), Query Frame = 0

Query: 48  AFLKIQLPNVPL-SEKRFGFFIFGNSFVDAGNNNYLNGTIRTRSNFPPYGESFFPIPTGR 107
           A L + LP   L S++    FIFG+S  D GNNN++N  +  ++NF PYG+S+F  PTGR
Sbjct: 15  AVLCLSLPTGCLSSQQAAALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPTGR 74

Query: 108 YCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGVNFGSGGAPILPESTNETALSLQTQI 167
           + DGRIIPDF+AEYA +P +P +L+PNN ++ +G NF S GA  L  S    A+ LQTQ+
Sbjct: 75  FSDGRIIPDFIAEYASLPIIPAYLEPNN-DFTHGANFASAGAGALIASHAGLAVGLQTQL 134

Query: 168 EFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGDILHPFESSFDIFNTIESQEQYAN 227
            +F  +    R+++G +  S+  LS++V+LF+ GG D   P+           +QEQY +
Sbjct: 135 RYFGDLVDHYRQNLG-DIKSRQLLSDAVYLFSCGGNDYQSPYYP--------YTQEQYVD 194

Query: 228 MVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRLAK-NEEFIQKSNSLSKVYNKLLL 287
           +VI NMT  +K IY  GGRKFGV+ V   G  P  R  +       + + L++++N+   
Sbjct: 195 IVIGNMTNVIKGIYEKGGRKFGVVNVPLIGCWPGMRAKQPGNTCNTEVDELTRLHNQAFA 254

Query: 288 IALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKVVDTACCGSDEFRGSYNCGRNTG 347
             L++L KQL+GF Y+  D     + R++NP+KYGFK  ++ACCGS  F G+Y+CGR   
Sbjct: 255 KRLEQLEKQLEGFVYAKFDLSTAILNRMKNPSKYGFKEGESACCGSGPFGGNYDCGR--- 314

Query: 348 TIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGVDIVKPYSFKQLFQ 397
              F  C N ++Y F+D +HP E A  QFA++ W G   + +PY+ K LF+
Sbjct: 315 IKEFGLCDNATEYFFFDPFHPNELASRQFAEMFWDGDSMVTQPYNLKALFE 352

BLAST of Cucsat.G16028 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.5e-68
Identity = 137/347 (39.48%), Postives = 207/347 (59.65%), Query Frame = 0

Query: 56  NVPLSEKRFGFFIFGNSFVDAGNNNYLNGTIRTRSNFPPYGESFFPIPTGRYCDGRIIPD 115
           N  L   +   F+FG+S  DAGNNNY++     RSN+ PYG++ F  PTGR  DGR IPD
Sbjct: 30  NNNLVTNQSALFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPD 89

Query: 116 FLAEYAGMPFLPPFLDPNN--SNYMNGVNFGSGGAPILPESTNETALSLQTQIEFFKIVE 175
           F+AEYA +P +P +L P+N  + +  GV+F S GA  L  +     ++L++Q+  FK VE
Sbjct: 90  FIAEYAWLPLIPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVE 149

Query: 176 KSIRKDMGNETLSQTFLSNSVFLFNIGGGDILHPFESSFDIFNTIESQEQYANMVINNMT 235
           K +R  +G E   +  +S +V+LF+IG  D  +PF ++  IF +   QE Y + V+ N T
Sbjct: 150 KLLRSTLG-EAQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQS-SPQEIYVDFVVGNTT 209

Query: 236 IALKEIYNLGGRKFGVLGVLPSGYLPSSRLAKNEEF---IQKSNSLSKVYNKLLLIALQK 295
             +KE+Y +GGRKFG L +      P+S +    +     +    L  ++N+ L   L++
Sbjct: 210 AVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRR 269

Query: 296 LVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKVVDTACCGSDEFRGSYNCGRNTG-TIPF 355
           L ++L GFKY+  D +     R+ NP+KYGFK    ACCG+   RG   CG   G +  +
Sbjct: 270 LERELSGFKYALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSY 329

Query: 356 SHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGVDIVKPYSFKQLFQ 397
             C+ ++DYLF+D +H TEKA++Q A+LIWSG  ++ KPY+ + LF+
Sbjct: 330 ELCEKVTDYLFFDHFHLTEKAHQQIAELIWSGPTNVTKPYNLQALFE 374

BLAST of Cucsat.G16028 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 259.2 bits (661), Expect = 7.6e-68
Identity = 147/372 (39.52%), Postives = 212/372 (56.99%), Query Frame = 0

Query: 31  ICFFFFFILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNYLNGTIRTRS 90
           I FF + I+  I +         + N  L   +   F+FG+S  DAGNNNY+N     RS
Sbjct: 7   IIFFVYTIILSIGSINC------IDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSFRS 66

Query: 91  NFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSN--YMNGVNFGSGGA 150
           N  PYG++ F  PTGR  DG        E A +P +PP L PNN N  +  GV+F S GA
Sbjct: 67  NIWPYGQTNFKFPTGRLSDG-------PEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGA 126

Query: 151 PILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGDILHPF 210
             L ES     ++L TQ+  FK VEKS+R ++G+    + F S +V+LF+IG  D  +PF
Sbjct: 127 GALAESFLGMVINLGTQLNNFKDVEKSLRSELGDAETKRVF-SRAVYLFHIGANDYFYPF 186

Query: 211 ESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRL---AK 270
            ++   F +  S+E++ + VI N+T  ++E+Y +GGRKFG L V P    P+S +    K
Sbjct: 187 SANSSTFKS-NSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTK 246

Query: 271 NEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKVVD 330
                +    L  ++NK     L++L +QL GF+Y+  D +    +RI +P+KYGFK   
Sbjct: 247 IGSCFKPVAELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYGFKEGK 306

Query: 331 TACCGSDEFRGSYNCGRNTG-TIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGVD 390
            ACCGS   RG   CG   G +  +  C+N++DYLFYDS H TEKA+ Q A+LIW+G  +
Sbjct: 307 KACCGSGPLRGINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNGPPN 363

Query: 391 IVKPYSFKQLFQ 397
           + +PY+ K LF+
Sbjct: 367 VTRPYNLKALFE 363

BLAST of Cucsat.G16028 vs. NCBI nr
Match: KAE8651958.1 (hypothetical protein Csa_006781 [Cucumis sativus])

HSP 1 Score: 827 bits (2136), Expect = 1.10e-302
Identity = 403/403 (100.00%), Postives = 403/403 (100.00%), Query Frame = 0

Query: 1   MWSIFIYCIYKNIISSPTHHKEEMKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLS 60
           MWSIFIYCIYKNIISSPTHHKEEMKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLS
Sbjct: 1   MWSIFIYCIYKNIISSPTHHKEEMKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLS 60

Query: 61  EKRFGFFIFGNSFVDAGNNNYLNGTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEY 120
           EKRFGFFIFGNSFVDAGNNNYLNGTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEY
Sbjct: 61  EKRFGFFIFGNSFVDAGNNNYLNGTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEY 120

Query: 121 AGMPFLPPFLDPNNSNYMNGVNFGSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDM 180
           AGMPFLPPFLDPNNSNYMNGVNFGSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDM
Sbjct: 121 AGMPFLPPFLDPNNSNYMNGVNFGSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDM 180

Query: 181 GNETLSQTFLSNSVFLFNIGGGDILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIY 240
           GNETLSQTFLSNSVFLFNIGGGDILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIY
Sbjct: 181 GNETLSQTFLSNSVFLFNIGGGDILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIY 240

Query: 241 NLGGRKFGVLGVLPSGYLPSSRLAKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKY 300
           NLGGRKFGVLGVLPSGYLPSSRLAKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKY
Sbjct: 241 NLGGRKFGVLGVLPSGYLPSSRLAKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKY 300

Query: 301 SYVDAYNFFMQRIQNPTKYGFKVVDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLF 360
           SYVDAYNFFMQRIQNPTKYGFKVVDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLF
Sbjct: 301 SYVDAYNFFMQRIQNPTKYGFKVVDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLF 360

Query: 361 YDSYHPTEKAYEQFAKLIWSGGVDIVKPYSFKQLFQSDSTFGV 403
           YDSYHPTEKAYEQFAKLIWSGGVDIVKPYSFKQLFQSDSTFGV
Sbjct: 361 YDSYHPTEKAYEQFAKLIWSGGVDIVKPYSFKQLFQSDSTFGV 403

BLAST of Cucsat.G16028 vs. NCBI nr
Match: XP_004151316.1 (GDSL esterase/lipase 1 [Cucumis sativus])

HSP 1 Score: 775 bits (2000), Expect = 2.47e-282
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 24  MKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNYLN 83
           MKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNYLN
Sbjct: 1   MKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNYLN 60

Query: 84  GTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGVNF 143
           GTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGVNF
Sbjct: 61  GTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGVNF 120

Query: 144 GSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGD 203
           GSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGD
Sbjct: 121 GSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGD 180

Query: 204 ILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRL 263
           ILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRL
Sbjct: 181 ILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRL 240

Query: 264 AKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKV 323
           AKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKV
Sbjct: 241 AKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKV 300

Query: 324 VDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGV 383
           VDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGV
Sbjct: 301 VDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGV 360

Query: 384 DIVKPYSFKQLFQSDSTFGV 403
           DIVKPYSFKQLFQSDSTFGV
Sbjct: 361 DIVKPYSFKQLFQSDSTFGV 380

BLAST of Cucsat.G16028 vs. NCBI nr
Match: XP_038901400.1 (GDSL lipase-like isoform X3 [Benincasa hispida])

HSP 1 Score: 522 bits (1345), Expect = 1.45e-182
Identity = 252/378 (66.67%), Postives = 307/378 (81.22%), Query Frame = 0

Query: 24  MKFPNLGICFFFF--FILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNY 83
           MK  NLGIC FFF  FILF IAA QSAFLKIQLP+V    KRFGFFIFG+S+VDAGNNNY
Sbjct: 1   MKISNLGICVFFFYIFILFGIAAPQSAFLKIQLPHVQSWGKRFGFFIFGDSYVDAGNNNY 60

Query: 84  LNGTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGV 143
           +N T   R+NFPPYGE+FFPIPTGR+ DGRIIPDFLAEYA MPF+  +LDPNN+NY NGV
Sbjct: 61  INLTSDCRANFPPYGETFFPIPTGRFSDGRIIPDFLAEYAQMPFIQAYLDPNNNNYTNGV 120

Query: 144 NFGSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGG 203
           NF SGGA  L E+  + A+ LQTQ+ FFK V+KS+RK +GNE  SQ+FLSNSVFLF+ GG
Sbjct: 121 NFASGGAGALVETNQKKAIGLQTQMMFFKRVKKSLRKKLGNER-SQSFLSNSVFLFHFGG 180

Query: 204 GDILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSS 263
            D L+P + ++DIF TIE+QEQ+ NMVI N+TIALKE+YN GGRKFG++ + P+GYLP+S
Sbjct: 181 NDYLNPLDINYDIFKTIEAQEQFVNMVIGNITIALKEVYNYGGRKFGIMALPPTGYLPNS 240

Query: 264 RLAKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGF 323
           R  ++E++ Q+S+S+++++N LL IALQKL  QL GFKY++ D + F +Q I NPTK+GF
Sbjct: 241 RWKRSEQYFQESSSITRIHNNLLFIALQKLATQLNGFKYAFADTHTFLLQTILNPTKFGF 300

Query: 324 KVVDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSG 383
           KVVDTACCGS EFRG Y+CGR    +PF+HC N+ DYLFYDSYHPTEKA EQ AKLIW+G
Sbjct: 301 KVVDTACCGSGEFRGIYSCGRKEEPLPFTHCTNLQDYLFYDSYHPTEKASEQLAKLIWNG 360

Query: 384 GVDIVKPYSFKQLFQSDS 399
             +IVKPY+FKQLFQ DS
Sbjct: 361 DDEIVKPYNFKQLFQYDS 377

BLAST of Cucsat.G16028 vs. NCBI nr
Match: XP_038901398.1 (GDSL lipase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 508 bits (1307), Expect = 2.27e-176
Identity = 252/405 (62.22%), Postives = 307/405 (75.80%), Query Frame = 0

Query: 24  MKFPNLGICFFFF--FILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNY 83
           MK  NLGIC FFF  FILF IAA QSAFLKIQLP+V    KRFGFFIFG+S+VDAGNNNY
Sbjct: 1   MKISNLGICVFFFYIFILFGIAAPQSAFLKIQLPHVQSWGKRFGFFIFGDSYVDAGNNNY 60

Query: 84  LNGTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGV 143
           +N T   R+NFPPYGE+FFPIPTGR+ DGRIIPDFLAEYA MPF+  +LDPNN+NY NGV
Sbjct: 61  INLTSDCRANFPPYGETFFPIPTGRFSDGRIIPDFLAEYAQMPFIQAYLDPNNNNYTNGV 120

Query: 144 NFGSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGG 203
           NF SGGA  L E+  + A+ LQTQ+ FFK V+KS+RK +GNE  SQ+FLSNSVFLF+ GG
Sbjct: 121 NFASGGAGALVETNQKKAIGLQTQMMFFKRVKKSLRKKLGNER-SQSFLSNSVFLFHFGG 180

Query: 204 GDILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSS 263
            D L+P + ++DIF TIE+QEQ+ NMVI N+TIALKE+YN GGRKFG++ + P+GYLP+S
Sbjct: 181 NDYLNPLDINYDIFKTIEAQEQFVNMVIGNITIALKEVYNYGGRKFGIMALPPTGYLPNS 240

Query: 264 RLAKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYG- 323
           R  ++E++ Q+S+S+++++N LL IALQKL  QL GFKY++ D + F +Q I NPTK+G 
Sbjct: 241 RWKRSEQYFQESSSITRIHNNLLFIALQKLATQLNGFKYAFADTHTFLLQTILNPTKFGE 300

Query: 324 --------------------------FKVVDTACCGSDEFRGSYNCGRNTGTIPFSHCKN 383
                                     FKVVDTACCGS EFRG Y+CGR    +PF+HC N
Sbjct: 301 STTRLVGVVCGRPLTKPFENTVKLFCFKVVDTACCGSGEFRGIYSCGRKEEPLPFTHCTN 360

Query: 384 ISDYLFYDSYHPTEKAYEQFAKLIWSGGVDIVKPYSFKQLFQSDS 399
           + DYLFYDSYHPTEKA EQ AKLIW+G  +IVKPY+FKQLFQ DS
Sbjct: 361 LQDYLFYDSYHPTEKASEQLAKLIWNGDDEIVKPYNFKQLFQYDS 404

BLAST of Cucsat.G16028 vs. NCBI nr
Match: XP_038900701.1 (GDSL lipase-like [Benincasa hispida])

HSP 1 Score: 489 bits (1260), Expect = 9.07e-170
Identity = 235/376 (62.50%), Postives = 297/376 (78.99%), Query Frame = 0

Query: 24  MKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNYLN 83
           MKFP LGICFF   ILF  A +++A  +IQLP +P SE RFG FIFG+S+VDAGNNNY+N
Sbjct: 1   MKFPKLGICFF---ILFS-AVSEAA--RIQLPGIPSSESRFGLFIFGDSYVDAGNNNYIN 60

Query: 84  GTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGVNF 143
            T   ++NFPPYGESFF IPTGR+ DGR IPDFL EYA +P LPP+LDP+N+ Y +GVNF
Sbjct: 61  TTSDFQANFPPYGESFFQIPTGRFTDGRNIPDFLGEYANLPLLPPYLDPHNNLYEHGVNF 120

Query: 144 GSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGD 203
            SGG   L  S  + A+ LQTQ++FFK VEKS+RK +G    S+TFLSNSVFLFN GG D
Sbjct: 121 ASGGGGALALSHQDQAIGLQTQLKFFKNVEKSLRKKLGYAR-SKTFLSNSVFLFNFGGND 180

Query: 204 ILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRL 263
            L+PF+ S+DIF TIE+QE Y NMVI N+TIA+KE+Y  GGRKFG L V P GY+PSSRL
Sbjct: 181 YLNPFDISYDIFKTIEAQEHYVNMVIGNITIAVKEVYKYGGRKFGFLAVPPLGYMPSSRL 240

Query: 264 AKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKV 323
            K+ +F ++++S+++++NKLL IALQKL KQLKGFKY++ D +   +QRI NPTKYGFK+
Sbjct: 241 KKSAQFFEEASSIARIHNKLLPIALQKLSKQLKGFKYTFADVHTLLLQRILNPTKYGFKI 300

Query: 324 VDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGV 383
           VDTACCGSDEFRG YNCGR  G +P++HC+N+ D++F+DS+HPT+K ++Q A+  WSG  
Sbjct: 301 VDTACCGSDEFRGVYNCGRKFGDLPYTHCENLEDHMFFDSFHPTQKVFKQLAEEFWSGDA 360

Query: 384 DIVKPYSFKQLFQSDS 399
           +IVKP +FKQLFQ DS
Sbjct: 361 EIVKPLNFKQLFQYDS 369

BLAST of Cucsat.G16028 vs. ExPASy TrEMBL
Match: A0A0A0LJN7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G296100 PE=3 SV=1)

HSP 1 Score: 775 bits (2000), Expect = 1.20e-282
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 24  MKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNYLN 83
           MKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNYLN
Sbjct: 1   MKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNYLN 60

Query: 84  GTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGVNF 143
           GTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGVNF
Sbjct: 61  GTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGVNF 120

Query: 144 GSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGD 203
           GSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGD
Sbjct: 121 GSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGD 180

Query: 204 ILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRL 263
           ILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRL
Sbjct: 181 ILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRL 240

Query: 264 AKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKV 323
           AKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKV
Sbjct: 241 AKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKV 300

Query: 324 VDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGV 383
           VDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGV
Sbjct: 301 VDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGV 360

Query: 384 DIVKPYSFKQLFQSDSTFGV 403
           DIVKPYSFKQLFQSDSTFGV
Sbjct: 361 DIVKPYSFKQLFQSDSTFGV 380

BLAST of Cucsat.G16028 vs. ExPASy TrEMBL
Match: A0A6J1EIS0 (GDSL esterase/lipase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111434936 PE=3 SV=1)

HSP 1 Score: 488 bits (1257), Expect = 1.67e-169
Identity = 235/383 (61.36%), Postives = 298/383 (77.81%), Query Frame = 0

Query: 24  MKFPNLGICFFFFFILFDIAAAQSAF------LKIQLPNVPLSEKRFGFFIFGNSFVDAG 83
           MKFPNLG CFFF  ILF   AAQ  F      + IQLP  P SE RFGFFIFG+S+VD G
Sbjct: 1   MKFPNLGSCFFF--ILF---AAQCGFPTISEAVGIQLPGAPSSENRFGFFIFGDSYVDVG 60

Query: 84  NNNYLNGTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNY 143
           NNNY+N T   ++NFPPYGESFFP+ TGR+ DGR IPDFL EYA +P LPP+LDP+N  Y
Sbjct: 61  NNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLY 120

Query: 144 MNGVNFGSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLF 203
             GVNF SGG   L  S  E AL LQTQ++FFK V+KS+RK +GN   +Q+F SNSVFLF
Sbjct: 121 DYGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNAR-TQSFFSNSVFLF 180

Query: 204 NIGGGDILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGY 263
           N GG D L+PF+ S+DIF TI++QEQ+ NMVI N+T ALKE+Y  GGRKFG++GV P GY
Sbjct: 181 NFGGNDYLNPFDISYDIFKTIDAQEQFVNMVIGNITKALKEVYKHGGRKFGLMGVPPLGY 240

Query: 264 LPSSRLAKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPT 323
           +PSSRL K+ +F ++++S+++++NKLL IAL KL KQLKGFKY++ D +   +QRI NP+
Sbjct: 241 MPSSRLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADTHTLLLQRILNPS 300

Query: 324 KYGFKVVDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKL 383
           +YGFKVVDTACCGSDEFRG YNCGR  G++PF+HCKN+ D++F+DS+HPTEK ++Q  + 
Sbjct: 301 RYGFKVVDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLTEQ 360

Query: 384 IWSGGVDIVKPYSFKQLFQSDST 400
           +WSGG+++VKPY+FKQLF  + T
Sbjct: 361 MWSGGLEVVKPYNFKQLFDYEPT 377

BLAST of Cucsat.G16028 vs. ExPASy TrEMBL
Match: A0A6J1EJ18 (GDSL esterase/lipase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434897 PE=3 SV=1)

HSP 1 Score: 483 bits (1243), Expect = 2.08e-167
Identity = 231/379 (60.95%), Postives = 289/379 (76.25%), Query Frame = 0

Query: 24  MKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNYLN 83
           MK   +G  FFFFFIL  IAAA S F  IQL ++P SE R GFFIFG+S+VDAGNNNY+N
Sbjct: 1   MKISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYIN 60

Query: 84  GTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGVNF 143
            T    +NFPPYGE+FF  PTGR+ DGRIIPDFLAEYA +P + P+LDP N+ Y+NGVNF
Sbjct: 61  TTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNF 120

Query: 144 GSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGD 203
            SGG   L  S    A+ LQTQ++FFK V+ S+ K +GN   +Q+F SNSVFLFN GG D
Sbjct: 121 ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNAR-TQSFFSNSVFLFNFGGND 180

Query: 204 ILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRL 263
            L+PF+ S+DIF T+++QEQ+A+MVI N+TIALKE+Y  GGRKFG + V P GY PSSRL
Sbjct: 181 YLNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRL 240

Query: 264 AKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKV 323
            KN +F  +++SL++V+NKL L+A+Q+L  QL GFKY++ D +   +QRI NPTKYGFK+
Sbjct: 241 KKNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKI 300

Query: 324 VDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGV 383
           VDTACCGSDEFRG +NCGR  G IP++ C+N+ DY+F+DSYHPTEK +EQ AK +WSG  
Sbjct: 301 VDTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDA 360

Query: 384 DIVKPYSFKQLFQSDSTFG 402
            IVKPY+FKQLFQ D   G
Sbjct: 361 HIVKPYNFKQLFQYDPFLG 378

BLAST of Cucsat.G16028 vs. ExPASy TrEMBL
Match: A0A6J1I9A0 (GDSL esterase/lipase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470360 PE=3 SV=1)

HSP 1 Score: 482 bits (1240), Expect = 6.16e-167
Identity = 233/383 (60.84%), Postives = 294/383 (76.76%), Query Frame = 0

Query: 24  MKFPNLGICFFFFFILFDIAAAQSAF------LKIQLPNVPLSEKRFGFFIFGNSFVDAG 83
           MKFPN   CFFF  ILF    AQ  F      + IQL   P SE RFGFFIFG+S+VDAG
Sbjct: 1   MKFPNRSSCFFF--ILF---VAQCGFPTISEAVGIQLTGAPSSENRFGFFIFGDSYVDAG 60

Query: 84  NNNYLNGTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNY 143
           NNNY+N T   ++NFPPYGESFFP+ TGR+ DGR IPDFL EYA +P LPP+LDP+N  Y
Sbjct: 61  NNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLY 120

Query: 144 MNGVNFGSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLF 203
             GVNF SGG   LP S  E AL LQTQ+ FFK V+KS+RK +GN   +Q+F SNSVFLF
Sbjct: 121 DYGVNFASGGGGALPLSHQEQALGLQTQMNFFKRVDKSLRKKLGNAR-TQSFFSNSVFLF 180

Query: 204 NIGGGDILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGY 263
           N GG D L+PF+ S+DIF TI++QEQ+ NMVI N+T  LKE+Y  GGRKFG++GV P GY
Sbjct: 181 NFGGNDYLNPFDISYDIFKTIDTQEQFVNMVIGNITTVLKEVYKHGGRKFGLMGVPPLGY 240

Query: 264 LPSSRLAKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPT 323
           +PSSRL K+ ++ ++++S+++++NKLL IAL KL KQLKGFKY++ D +   +QRI NP+
Sbjct: 241 MPSSRLKKSAQYFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADVHMLLLQRILNPS 300

Query: 324 KYGFKVVDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKL 383
           +YGFKVVDTACCGSDEFRG YNCGR  G+ PF+HCKNI D++F+DSYHPTEK ++Q A+ 
Sbjct: 301 QYGFKVVDTACCGSDEFRGVYNCGRKLGSFPFTHCKNIEDHMFFDSYHPTEKVFKQLAEQ 360

Query: 384 IWSGGVDIVKPYSFKQLFQSDST 400
           +WSGG+++V PY+FKQLF+ + T
Sbjct: 361 MWSGGLEVVTPYNFKQLFEYEPT 377

BLAST of Cucsat.G16028 vs. ExPASy TrEMBL
Match: A0A6J1I3Y7 (GDSL esterase/lipase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111470363 PE=3 SV=1)

HSP 1 Score: 471 bits (1211), Expect = 1.46e-162
Identity = 225/375 (60.00%), Postives = 288/375 (76.80%), Query Frame = 0

Query: 24  MKFPNLGICFFFFFILFDIAAAQSAFLKIQLPNVPLSEKRFGFFIFGNSFVDAGNNNYLN 83
           MK   +G  FFFFFIL   AAA S F  IQLP++P SE R GFFIFG+S+VDAGNNNY+N
Sbjct: 1   MKISKMG-SFFFFFILLGNAAAGSGFPGIQLPDIPSSENRSGFFIFGDSYVDAGNNNYIN 60

Query: 84  GTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSNYMNGVNF 143
            T    +N+PPYGE+FF  PTGR+ DGRIIPDFLAEYA +P + P+LDP N+ Y+NGVNF
Sbjct: 61  TTGDFLANYPPYGETFFTTPTGRFSDGRIIPDFLAEYAKLPLIQPYLDPKNNLYLNGVNF 120

Query: 144 GSGGAPILPESTNETALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVFLFNIGGGD 203
            SGG   L ES    A+ LQTQ++FFK V+ S+ K +G+   +++F SNSVFLFN GG D
Sbjct: 121 ASGGGGALAESHQGQAIGLQTQMKFFKRVKNSLTKRLGSAR-TRSFFSNSVFLFNFGGND 180

Query: 204 ILHPFESSFDIFNTIESQEQYANMVINNMTIALKEIYNLGGRKFGVLGVLPSGYLPSSRL 263
            L+PF+ S+DIF T+++QEQ+ANMVI N+TIALKE+Y  GGRKFG + V P GY PSSRL
Sbjct: 181 YLNPFDISYDIFKTLDAQEQFANMVIGNITIALKEVYKCGGRKFGFMTVPPFGYTPSSRL 240

Query: 264 AKNEEFIQKSNSLSKVYNKLLLIALQKLVKQLKGFKYSYVDAYNFFMQRIQNPTKYGFKV 323
            K+ +F  +++SL++V+NKL L+A+Q+L  QL GFKY++ D +   ++RI NPTKYGFK+
Sbjct: 241 KKSAQFFYETSSLARVHNKLFLMAIQRLATQLHGFKYAFADTHTLLLRRILNPTKYGFKI 300

Query: 324 VDTACCGSDEFRGSYNCGRNTGTIPFSHCKNISDYLFYDSYHPTEKAYEQFAKLIWSGGV 383
           +DTACCGSDEFRG +NCGR  G +P++ C+NI DY+F+DSYHPTEK +EQ A  +WSG  
Sbjct: 301 IDTACCGSDEFRGFFNCGRKKGALPYTLCENIQDYMFFDSYHPTEKTFEQLANEMWSGDA 360

Query: 384 DIVKPYSFKQLFQSD 398
           DIVK Y+FKQLFQ D
Sbjct: 361 DIVKSYNFKQLFQYD 373