Cucsat.G16012 (gene) Cucumber (B10) v3

BLAST of Cucsat.G16012 vs. ExPASy Swiss-Prot
Match: O80470 (GDSL esterase/lipase At2g23540 OS=Arabidopsis thaliana OX=3702 GN=At2g23540 PE=2 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.1e-161
Identity = 269/354 (75.99%), Postives = 311/354 (87.85%), Query Frame = 0

Query: 33  GATANNNKK--AVGASFIFGDSLVDAGNNNYLPTLSKANLRPNGMDYKPSGGKPTGRFTN 92
           G   NN+KK   +GASFIFGDSLVDAGNNNYL TLS+AN++PNG+D+K SGG PTGRFTN
Sbjct: 34  GRGVNNDKKGGGLGASFIFGDSLVDAGNNNYLSTLSRANMKPNGIDFKASGGTPTGRFTN 93

Query: 93  GRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGVNYASGGGGILNATGRIFVNRLGMDV 152
           GRTIGDIVGEELG  N+A+PFL P+A GK++L GVNYASGGGGI+NATGRIFVNRLGMDV
Sbjct: 94  GRTIGDIVGEELGSANYAIPFLAPDAKGKALLAGVNYASGGGGIMNATGRIFVNRLGMDV 153

Query: 153 QVDFFNVTRKQFDKIMGAEKAKEYIGKKSIFSITIGANDFLNNYLLPVLSVGARISQTPD 212
           QVDFFN TRKQFD ++G EKAK+YI KKSIFSITIGANDFLNNYL P+LSVG R +QTPD
Sbjct: 154 QVDFFNTTRKQFDDLLGKEKAKDYIAKKSIFSITIGANDFLNNYLFPLLSVGTRFTQTPD 213

Query: 213 AFVDDMISHLKNQLTRLYKMDGRKFVVGNVGPIGCIPYQKTINQLNEDECVDLANKLALQ 272
            F+ DM+ HL++QLTRLY++D RKFV+GNVGPIGCIPYQKTINQL+E+ECVDLANKLA Q
Sbjct: 214 DFIGDMLEHLRDQLTRLYQLDARKFVIGNVGPIGCIPYQKTINQLDENECVDLANKLANQ 273

Query: 273 YNAKLKDLLSSLNKDLPSSTFVYANVYDLVMDLIVNYDNYGFKTASRACCGNGGQFAGII 332
           YN +LK LL  LNK LP + FV+ANVYDLVM+LI NYD YGFK+A++ACCGNGGQ+AGII
Sbjct: 274 YNVRLKSLLEELNKKLPGAMFVHANVYDLVMELITNYDKYGFKSATKACCGNGGQYAGII 333

Query: 333 PCGPQSSLCSERSRHVFWDPYHPSEAANLLIAKKLLDGDHKFISPYNLRQLRDL 385
           PCGP SSLC ER ++VFWDPYHPSEAAN++IAK+LL GD K ISP NL +LRD+
Sbjct: 334 PCGPTSSLCEERDKYVFWDPYHPSEAANVIIAKQLLYGDVKVISPVNLSKLRDM 387

BLAST of Cucsat.G16012 vs. ExPASy Swiss-Prot
Match: Q9M2R9 (GDSL esterase/lipase At3g50400 OS=Arabidopsis thaliana OX=3702 GN=At3g50400 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 7.1e-148
Identity = 245/347 (70.61%), Postives = 299/347 (86.17%), Query Frame = 0

Query: 39  NKKAVGASFIFGDSLVDAGNNNYLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIV 98
           +++A+ ASF+FGDSLVDAGNNNYL TLS+AN  PNG+D+KPS G PTGRFTNGRTI DIV
Sbjct: 28  DQRALAASFVFGDSLVDAGNNNYLQTLSRANSPPNGIDFKPSRGNPTGRFTNGRTIADIV 87

Query: 99  GEELGIPNHAVPFLDPNATGKSILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVT 158
           GE+LG  ++AVP+L PNA+G+++L GVNYASGGGGILNATG +FVNRLGMD+QVD+F  T
Sbjct: 88  GEKLGQQSYAVPYLAPNASGEALLNGVNYASGGGGILNATGSVFVNRLGMDIQVDYFTNT 147

Query: 159 RKQFDKIMGAEKAKEYIGKKSIFSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMIS 218
           RKQFDK++G +KA++YI K+S+FS+ IG+NDFLNNYL+P ++  AR++QTP+ FVDDMIS
Sbjct: 148 RKQFDKLLGQDKARDYIRKRSLFSVVIGSNDFLNNYLVPFVAAQARLTQTPETFVDDMIS 207

Query: 219 HLKNQLTRLYKMDGRKFVVGNVGPIGCIPYQKTINQLNEDECVDLANKLALQYNAKLKDL 278
           HL+NQL RLY MD RKFVVGNV PIGCIPYQK+INQLN+ +CVDLANKLA+QYNA+LKDL
Sbjct: 208 HLRNQLKRLYDMDARKFVVGNVAPIGCIPYQKSINQLNDKQCVDLANKLAIQYNARLKDL 267

Query: 279 LS-SLNKDLPSSTFVYANVYDLVMDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSS 338
           L+  L   L  + FVYANVYDL MDLIVN+ +YGF+TAS ACC   G+ AGI+PCGP SS
Sbjct: 268 LTVELKDSLKDAHFVYANVYDLFMDLIVNFKDYGFRTASEACCETRGRLAGILPCGPTSS 327

Query: 339 LCSERSRHVFWDPYHPSEAANLLIAKKLLDGDHKFISPYNLRQLRDL 385
           LC++RS+HVFWD YHP+EAANLLIA KLL GD KF++P+NL  LRDL
Sbjct: 328 LCTDRSKHVFWDAYHPTEAANLLIADKLLYGDSKFVTPFNLLHLRDL 374

BLAST of Cucsat.G16012 vs. ExPASy Swiss-Prot
Match: O23470 (GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3 SV=2)

HSP 1 Score: 362.5 bits (929), Expect = 6.1e-99
Identity = 177/344 (51.45%), Postives = 237/344 (68.90%), Query Frame = 0

Query: 41  KAVGASFIFGDSLVDAGNNNYLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGE 100
           K + A+F+FGDSLVDAGNNNYL TLSKAN  PNG+D+    G PTGRFTNGRTI DIV +
Sbjct: 26  KKIPANFVFGDSLVDAGNNNYLATLSKANYVPNGIDF----GSPTGRFTNGRTIVDIVYQ 85

Query: 101 ELGIPNHAVPFLDPNATGKSILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRK 160
            LG      P+L P  +G  IL GVNYASGG GILN+TG++F  R+ +D Q+D F  TR+
Sbjct: 86  ALGSDELTPPYLAPTTSGSLILNGVNYASGGSGILNSTGKLFGERINVDAQLDNFATTRQ 145

Query: 161 QFDKIMGAEKAKEYIGKKSIFSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHL 220
                +G  +A + + + +IFS+T G+ND +NNY  PV+S   R    P+ FVD MIS  
Sbjct: 146 DIISWIGESEAAK-LFRSAIFSVTTGSNDLINNYFTPVISTLQRKVVAPEVFVDTMISKF 205

Query: 221 KNQLTRLYKMDGRKFVVGNVGPIGCIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLS 280
           + QLTRLY++  RK VV N+GPIGCIP+++  +    + C+   N++A  YN KLK L+ 
Sbjct: 206 RLQLTRLYQLGARKIVVINIGPIGCIPFERESDPAAGNNCLAEPNEVAQMYNLKLKTLVE 265

Query: 281 SLNKDLPSSTFVYANVYDLVMDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCS 340
            LNK+L  S FVY +V+ +V D+I NY +YGF++    CC   G+  G+IPCGP S +C 
Sbjct: 266 ELNKNLQGSRFVYGDVFRIVDDIIQNYSSYGFESEKIPCCSLVGKVGGLIPCGPPSKVCM 325

Query: 341 ERSRHVFWDPYHPSEAANLLIAKKLLDGDHKFISPYNLRQLRDL 385
           +RS++VFWDPYHP+EAAN++IA++LL GD   I P N+RQL +L
Sbjct: 326 DRSKYVFWDPYHPTEAANIIIARRLLSGDTSDIYPINIRQLANL 364

BLAST of Cucsat.G16012 vs. ExPASy Swiss-Prot
Match: Q9FJ25 (GDSL esterase/lipase At5g41890 OS=Arabidopsis thaliana OX=3702 GN=At5g41890 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 5.9e-86
Identity = 166/359 (46.24%), Postives = 228/359 (63.51%), Query Frame = 0

Query: 36  ANNNKKAVGASFIFGDSLVDAGNNNYLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIG 95
           +NN+      +FIFGDSLVD GNNNY+ TLSKA+  P G+D+ PS G+PTGRFTNGRTI 
Sbjct: 16  SNNSAAQSFTNFIFGDSLVDVGNNNYIFTLSKADSSPYGIDFAPSNGQPTGRFTNGRTIS 75

Query: 96  DIVGEELGIPNHAVPFLDPNATGKSILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFF 155
           DIVGE LG  +   P+L+PN    +I  G+NYASG  GIL+ TG +F+ R+ +  QV  F
Sbjct: 76  DIVGEALGAKSPPPPYLEPNTEANTIRNGINYASGAAGILDDTGLLFIGRVPLREQVSNF 135

Query: 156 NVTRKQFDKIMGAEKAKEYIGKKSIFSITIGANDFLNNYLLPVLSVGARISQTPDAFVDD 215
             +R+   +++G    KE + K ++F+ITIG+ND L NY+ P +   ++     D   D 
Sbjct: 136 EKSREYMVRVIGENGTKEML-KNAMFTITIGSNDIL-NYIQPSIPFFSQDKLPTDVLQDS 195

Query: 216 MISHLKNQLTRLYKMDGRKFVVGNVGPIGCIPYQKTINQLNEDECVDLANKLALQYNAKL 275
           M+ HL   L RL+++ GRKFVV  VGP+GCIP+ + +N +   +C +  N++   YN KL
Sbjct: 196 MVLHLTTHLKRLHQLGGRKFVVVGVGPLGCIPFARALNLIPAGKCSEQVNQVVRGYNMKL 255

Query: 276 KDLLSSLNKDLPS----STFVYANVYDLVMDLIVNYDNYGFKTASRACCGNGGQFAGIIP 335
              L +LN +L S    +TFVYAN YDL + L++NY  +G K A + CC  GG F     
Sbjct: 256 IHSLKTLNNELRSEDYNTTFVYANSYDLFLKLVLNYQLFGLKNADKPCC--GGYFPP-FA 315

Query: 336 C--GP----QSSLCSERSRHVFWDPYHPSEAANLLIAKKLLDGDHKFISPYNLRQLRDL 385
           C  GP      + C +RS+ VFWD YHP+EAANL++AK LLDGD    +P+N+R L DL
Sbjct: 316 CFKGPNQNSSQAACEDRSKFVFWDAYHPTEAANLIVAKALLDGDQTVATPFNIRYLNDL 369

BLAST of Cucsat.G16012 vs. ExPASy Swiss-Prot
Match: Q9FNP2 (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.2e-73
Identity = 158/386 (40.93%), Postives = 224/386 (58.03%), Query Frame = 0

Query: 3   EMMMRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNNYL 62
           +MMM  C +  L++V   L+  +L+     G  ++       A F+FGDSLVD GNNN+L
Sbjct: 11  DMMMMSCTVQTLVLVPWFLVVFVLAG----GEDSSETTAMFPAMFVFGDSLVDNGNNNHL 70

Query: 63  PTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVP-FLDPNATGKSI 122
            +L+++N  P G+D+  +G +PTGRF+NG+TI D +GE LG+P   +P F+D    G  I
Sbjct: 71  NSLARSNYLPYGIDF--AGNQPTGRFSNGKTIVDFIGELLGLPE--IPAFMDTVDGGVDI 130

Query: 123 LYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSIF 182
           L+GVNYAS  GGIL  TGR    R  M  QV+ F  T  +  + M  E  KEY+  KS+ 
Sbjct: 131 LHGVNYASAAGGILEETGRHLGERFSMGRQVENFEKTLMEISRSMRKESVKEYMA-KSLV 190

Query: 183 SITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNVG 242
            +++G ND++NNYL P L + + I   P +F D ++S+    L  LY    RKFV+  VG
Sbjct: 191 VVSLGNNDYINNYLKPRLFLSSSI-YDPTSFADLLLSNFTTHLLELYGKGFRKFVIAGVG 250

Query: 243 PIGCIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSST---FVYANVYD 302
           P+GCIP Q         ECV+  N++A  +N +L  L+  LN D  +++   FVY N Y 
Sbjct: 251 PLGCIPDQLAAQAALPGECVEAVNEMAELFNNRLVSLVDRLNSDNKTASEAIFVYGNTYG 310

Query: 303 LVMDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAAN 362
             +D++ N  NYGF+   R CCG  G+  G I C P +  C+ R RHVFWD +HP++A N
Sbjct: 311 AAVDILTNPFNYGFEVTDRGCCG-VGRNRGEITCLPLAVPCAFRDRHVFWDAFHPTQAFN 370

Query: 363 LLIAKKLLDGDHKFISPYNLRQLRDL 385
           L+IA +  +G      P NL QL  L
Sbjct: 371 LIIALRAFNGSKSDCYPINLSQLSRL 385

BLAST of Cucsat.G16012 vs. NCBI nr
Match: XP_004148069.2 (GDSL esterase/lipase At2g23540 [Cucumis sativus] >KAE8651938.1 hypothetical protein Csa_006363 [Cucumis sativus])

HSP 1 Score: 765 bits (1976), Expect = 5.43e-279
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 4   MMMRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNNYLP 63
           MMMRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNNYLP
Sbjct: 1   MMMRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNNYLP 60

Query: 64  TLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILY 123
           TLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILY
Sbjct: 61  TLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILY 120

Query: 124 GVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSIFSI 183
           GVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSIFSI
Sbjct: 121 GVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSIFSI 180

Query: 184 TIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNVGPI 243
           TIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNVGPI
Sbjct: 181 TIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNVGPI 240

Query: 244 GCIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLVMDL 303
           GCIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLVMDL
Sbjct: 241 GCIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLVMDL 300

Query: 304 IVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLLIAK 363
           IVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLLIAK
Sbjct: 301 IVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLLIAK 360

Query: 364 KLLDGDHKFISPYNLRQLRDL 384
           KLLDGDHKFISPYNLRQLRDL
Sbjct: 361 KLLDGDHKFISPYNLRQLRDL 381

BLAST of Cucsat.G16012 vs. NCBI nr
Match: XP_008457861.1 (PREDICTED: GDSL esterase/lipase At2g23540 [Cucumis melo] >TYK13727.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 741 bits (1913), Expect = 2.26e-269
Identity = 371/384 (96.61%), Postives = 378/384 (98.44%), Query Frame = 0

Query: 1   MVEMMMRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNN 60
           MVE+MMRGCKMGLLI VGLILMECLLSNNGVDGA  NN KKAVGASFIFGDSLVDAGNNN
Sbjct: 1   MVEIMMRGCKMGLLI-VGLILMECLLSNNGVDGAN-NNKKKAVGASFIFGDSLVDAGNNN 60

Query: 61  YLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKS 120
           YLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKS
Sbjct: 61  YLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKS 120

Query: 121 ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSI 180
           ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDK+MGAEKAKEYIGK+SI
Sbjct: 121 ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKMMGAEKAKEYIGKRSI 180

Query: 181 FSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNV 240
           FSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMD RKFVVGNV
Sbjct: 181 FSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDARKFVVGNV 240

Query: 241 GPIGCIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLV 300
           GPIGCIPYQKTINQLNED+CVDLANKLALQYNAKLKDLL++LNKDLPSSTFVYANVYDLV
Sbjct: 241 GPIGCIPYQKTINQLNEDQCVDLANKLALQYNAKLKDLLANLNKDLPSSTFVYANVYDLV 300

Query: 301 MDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLL 360
           MDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANL+
Sbjct: 301 MDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLI 360

Query: 361 IAKKLLDGDHKFISPYNLRQLRDL 384
           IAKKLLDGDHKFI PYNLRQLRDL
Sbjct: 361 IAKKLLDGDHKFIFPYNLRQLRDL 382

BLAST of Cucsat.G16012 vs. NCBI nr
Match: XP_038902261.1 (GDSL esterase/lipase At2g23540 [Benincasa hispida])

HSP 1 Score: 702 bits (1813), Expect = 3.64e-254
Identity = 349/384 (90.89%), Postives = 368/384 (95.83%), Query Frame = 0

Query: 1   MVEMMMRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNN 60
           MV M+M+GCKMGLLI VGLI M CLLSNNGVDGA   N K AVGASFIFGDSLVDAGNNN
Sbjct: 1   MVVMIMKGCKMGLLI-VGLISMGCLLSNNGVDGA---NKKVAVGASFIFGDSLVDAGNNN 60

Query: 61  YLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKS 120
           YLPTLSKANLRPNG+DYKPSGG PTGRFTNGRTIGDIVGEELGIPNHAVP+LDPNATGKS
Sbjct: 61  YLPTLSKANLRPNGIDYKPSGGNPTGRFTNGRTIGDIVGEELGIPNHAVPYLDPNATGKS 120

Query: 121 ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSI 180
           ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRK+FDK+MG  KAKEYI KKSI
Sbjct: 121 ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKEFDKLMGVPKAKEYIAKKSI 180

Query: 181 FSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNV 240
           FSITIGANDFLNNYLLPVLS+GARISQTPD+F+DDMISHLKNQLTRLY+MD RKFVVGNV
Sbjct: 181 FSITIGANDFLNNYLLPVLSIGARISQTPDSFIDDMISHLKNQLTRLYQMDARKFVVGNV 240

Query: 241 GPIGCIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLV 300
           GPIGCIPYQKTINQLNED+CVDLANKLALQYNAKLKDLL++LNK+LP+STFVYANVYDLV
Sbjct: 241 GPIGCIPYQKTINQLNEDQCVDLANKLALQYNAKLKDLLANLNKNLPTSTFVYANVYDLV 300

Query: 301 MDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLL 360
           M+LIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLC+ERSRHVFWDPYHPSEAANL+
Sbjct: 301 MELIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCTERSRHVFWDPYHPSEAANLI 360

Query: 361 IAKKLLDGDHKFISPYNLRQLRDL 384
           IAKKLLDGDHK+I P NLRQLRDL
Sbjct: 361 IAKKLLDGDHKYIFPLNLRQLRDL 380

BLAST of Cucsat.G16012 vs. NCBI nr
Match: XP_022971744.1 (GDSL esterase/lipase At2g23540 [Cucurbita maxima])

HSP 1 Score: 679 bits (1753), Expect = 4.19e-245
Identity = 337/379 (88.92%), Postives = 354/379 (93.40%), Query Frame = 0

Query: 6   MRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNNYLPTL 65
           M GCKM    IVGLILM CLL NNGVDGA   N K  VGASFIFGDSLVDAGNNNYL TL
Sbjct: 4   MGGCKM----IVGLILMGCLLVNNGVDGA---NKKVDVGASFIFGDSLVDAGNNNYLSTL 63

Query: 66  SKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV 125
           SKANLRPNG+DY PSGG PTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV
Sbjct: 64  SKANLRPNGIDYTPSGGNPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV 123

Query: 126 NYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSIFSITI 185
           NYASGGGGILNATGRIFVNRLGMDVQVDFFN+TRKQFDKI+G  KA++YI KKSI SITI
Sbjct: 124 NYASGGGGILNATGRIFVNRLGMDVQVDFFNITRKQFDKILGPSKARDYISKKSIISITI 183

Query: 186 GANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNVGPIGC 245
           GANDFLNNYLLPVLS+GARISQ+PD+F+DDMI+HLKNQLTRLY+MD RKFVVGNVGPIGC
Sbjct: 184 GANDFLNNYLLPVLSIGARISQSPDSFIDDMIAHLKNQLTRLYQMDARKFVVGNVGPIGC 243

Query: 246 IPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLVMDLIV 305
           IPYQKTINQLNED+CVDLANKLALQYNAKLKDLL+ LNK LP+STFVYANVYDLVMDLIV
Sbjct: 244 IPYQKTINQLNEDQCVDLANKLALQYNAKLKDLLADLNKKLPASTFVYANVYDLVMDLIV 303

Query: 306 NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLLIAKKL 365
           NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLC+ERSRHVFWDPYHPSEAANL+IAKKL
Sbjct: 304 NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCTERSRHVFWDPYHPSEAANLIIAKKL 363

Query: 366 LDGDHKFISPYNLRQLRDL 384
           LDGDHK+I P NLRQLRDL
Sbjct: 364 LDGDHKYIFPLNLRQLRDL 375

BLAST of Cucsat.G16012 vs. NCBI nr
Match: KAG7011175.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 676 bits (1745), Expect = 6.20e-244
Identity = 335/379 (88.39%), Postives = 354/379 (93.40%), Query Frame = 0

Query: 6   MRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNNYLPTL 65
           M GCKM    IV LILM CLL NNGVDGA   N K  VGASFIFGDSLVDAGNNNYL TL
Sbjct: 1   MGGCKM----IVRLILMGCLLVNNGVDGA---NKKVDVGASFIFGDSLVDAGNNNYLSTL 60

Query: 66  SKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV 125
           SKANLRPNG+DY PSGG PTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV
Sbjct: 61  SKANLRPNGIDYTPSGGNPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV 120

Query: 126 NYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSIFSITI 185
           NYASGGGGILNATGRIFVNRLGMDVQVDFFN+TRKQFDK++G  KA++YI KKSI SITI
Sbjct: 121 NYASGGGGILNATGRIFVNRLGMDVQVDFFNITRKQFDKLLGPSKARDYISKKSIISITI 180

Query: 186 GANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNVGPIGC 245
           GANDFLNNYLLPVLS+GARISQ+PD+F+DDMI+HLKNQLTRLY+MD RKFVVGNVGPIGC
Sbjct: 181 GANDFLNNYLLPVLSIGARISQSPDSFIDDMIAHLKNQLTRLYQMDARKFVVGNVGPIGC 240

Query: 246 IPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLVMDLIV 305
           IPYQKTINQLNED+CVDLANKLALQYNAKLKDLL+ LNK+LP+STFVYANVYDLVMDLIV
Sbjct: 241 IPYQKTINQLNEDQCVDLANKLALQYNAKLKDLLADLNKNLPASTFVYANVYDLVMDLIV 300

Query: 306 NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLLIAKKL 365
           NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLC+ERSRHVFWDPYHPSEAANL+IAKKL
Sbjct: 301 NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCTERSRHVFWDPYHPSEAANLIIAKKL 360

Query: 366 LDGDHKFISPYNLRQLRDL 384
           LDGDHK+I P NLRQLRDL
Sbjct: 361 LDGDHKYIFPLNLRQLRDL 372

BLAST of Cucsat.G16012 vs. ExPASy TrEMBL
Match: A0A0A0LJV4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292780 PE=3 SV=1)

HSP 1 Score: 764 bits (1973), Expect = 7.25e-279
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 5   MMRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNNYLPT 64
           MMRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNNYLPT
Sbjct: 1   MMRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNNYLPT 60

Query: 65  LSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYG 124
           LSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYG
Sbjct: 61  LSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYG 120

Query: 125 VNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSIFSIT 184
           VNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSIFSIT
Sbjct: 121 VNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSIFSIT 180

Query: 185 IGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNVGPIG 244
           IGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNVGPIG
Sbjct: 181 IGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNVGPIG 240

Query: 245 CIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLVMDLI 304
           CIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLVMDLI
Sbjct: 241 CIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLVMDLI 300

Query: 305 VNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLLIAKK 364
           VNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLLIAKK
Sbjct: 301 VNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLLIAKK 360

Query: 365 LLDGDHKFISPYNLRQLRDL 384
           LLDGDHKFISPYNLRQLRDL
Sbjct: 361 LLDGDHKFISPYNLRQLRDL 380

BLAST of Cucsat.G16012 vs. ExPASy TrEMBL
Match: A0A5D3CPB1 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002550 PE=3 SV=1)

HSP 1 Score: 741 bits (1913), Expect = 1.09e-269
Identity = 371/384 (96.61%), Postives = 378/384 (98.44%), Query Frame = 0

Query: 1   MVEMMMRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNN 60
           MVE+MMRGCKMGLLI VGLILMECLLSNNGVDGA  NN KKAVGASFIFGDSLVDAGNNN
Sbjct: 1   MVEIMMRGCKMGLLI-VGLILMECLLSNNGVDGAN-NNKKKAVGASFIFGDSLVDAGNNN 60

Query: 61  YLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKS 120
           YLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKS
Sbjct: 61  YLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKS 120

Query: 121 ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSI 180
           ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDK+MGAEKAKEYIGK+SI
Sbjct: 121 ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKMMGAEKAKEYIGKRSI 180

Query: 181 FSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNV 240
           FSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMD RKFVVGNV
Sbjct: 181 FSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDARKFVVGNV 240

Query: 241 GPIGCIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLV 300
           GPIGCIPYQKTINQLNED+CVDLANKLALQYNAKLKDLL++LNKDLPSSTFVYANVYDLV
Sbjct: 241 GPIGCIPYQKTINQLNEDQCVDLANKLALQYNAKLKDLLANLNKDLPSSTFVYANVYDLV 300

Query: 301 MDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLL 360
           MDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANL+
Sbjct: 301 MDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLI 360

Query: 361 IAKKLLDGDHKFISPYNLRQLRDL 384
           IAKKLLDGDHKFI PYNLRQLRDL
Sbjct: 361 IAKKLLDGDHKFIFPYNLRQLRDL 382

BLAST of Cucsat.G16012 vs. ExPASy TrEMBL
Match: A0A1S3C7S2 (GDSL esterase/lipase At2g23540 OS=Cucumis melo OX=3656 GN=LOC103497446 PE=3 SV=1)

HSP 1 Score: 741 bits (1913), Expect = 1.09e-269
Identity = 371/384 (96.61%), Postives = 378/384 (98.44%), Query Frame = 0

Query: 1   MVEMMMRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNN 60
           MVE+MMRGCKMGLLI VGLILMECLLSNNGVDGA  NN KKAVGASFIFGDSLVDAGNNN
Sbjct: 1   MVEIMMRGCKMGLLI-VGLILMECLLSNNGVDGAN-NNKKKAVGASFIFGDSLVDAGNNN 60

Query: 61  YLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKS 120
           YLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKS
Sbjct: 61  YLPTLSKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKS 120

Query: 121 ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSI 180
           ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDK+MGAEKAKEYIGK+SI
Sbjct: 121 ILYGVNYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKMMGAEKAKEYIGKRSI 180

Query: 181 FSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNV 240
           FSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMD RKFVVGNV
Sbjct: 181 FSITIGANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDARKFVVGNV 240

Query: 241 GPIGCIPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLV 300
           GPIGCIPYQKTINQLNED+CVDLANKLALQYNAKLKDLL++LNKDLPSSTFVYANVYDLV
Sbjct: 241 GPIGCIPYQKTINQLNEDQCVDLANKLALQYNAKLKDLLANLNKDLPSSTFVYANVYDLV 300

Query: 301 MDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLL 360
           MDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANL+
Sbjct: 301 MDLIVNYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLI 360

Query: 361 IAKKLLDGDHKFISPYNLRQLRDL 384
           IAKKLLDGDHKFI PYNLRQLRDL
Sbjct: 361 IAKKLLDGDHKFIFPYNLRQLRDL 382

BLAST of Cucsat.G16012 vs. ExPASy TrEMBL
Match: A0A6J1I9F4 (GDSL esterase/lipase At2g23540 OS=Cucurbita maxima OX=3661 GN=LOC111470411 PE=3 SV=1)

HSP 1 Score: 679 bits (1753), Expect = 2.03e-245
Identity = 337/379 (88.92%), Postives = 354/379 (93.40%), Query Frame = 0

Query: 6   MRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNNYLPTL 65
           M GCKM    IVGLILM CLL NNGVDGA   N K  VGASFIFGDSLVDAGNNNYL TL
Sbjct: 4   MGGCKM----IVGLILMGCLLVNNGVDGA---NKKVDVGASFIFGDSLVDAGNNNYLSTL 63

Query: 66  SKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV 125
           SKANLRPNG+DY PSGG PTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV
Sbjct: 64  SKANLRPNGIDYTPSGGNPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV 123

Query: 126 NYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSIFSITI 185
           NYASGGGGILNATGRIFVNRLGMDVQVDFFN+TRKQFDKI+G  KA++YI KKSI SITI
Sbjct: 124 NYASGGGGILNATGRIFVNRLGMDVQVDFFNITRKQFDKILGPSKARDYISKKSIISITI 183

Query: 186 GANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNVGPIGC 245
           GANDFLNNYLLPVLS+GARISQ+PD+F+DDMI+HLKNQLTRLY+MD RKFVVGNVGPIGC
Sbjct: 184 GANDFLNNYLLPVLSIGARISQSPDSFIDDMIAHLKNQLTRLYQMDARKFVVGNVGPIGC 243

Query: 246 IPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLVMDLIV 305
           IPYQKTINQLNED+CVDLANKLALQYNAKLKDLL+ LNK LP+STFVYANVYDLVMDLIV
Sbjct: 244 IPYQKTINQLNEDQCVDLANKLALQYNAKLKDLLADLNKKLPASTFVYANVYDLVMDLIV 303

Query: 306 NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLLIAKKL 365
           NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLC+ERSRHVFWDPYHPSEAANL+IAKKL
Sbjct: 304 NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCTERSRHVFWDPYHPSEAANLIIAKKL 363

Query: 366 LDGDHKFISPYNLRQLRDL 384
           LDGDHK+I P NLRQLRDL
Sbjct: 364 LDGDHKYIFPLNLRQLRDL 375

BLAST of Cucsat.G16012 vs. ExPASy TrEMBL
Match: A0A6J1EIP8 (GDSL esterase/lipase At2g23540 OS=Cucurbita moschata OX=3662 GN=LOC111434898 PE=3 SV=1)

HSP 1 Score: 675 bits (1742), Expect = 9.63e-244
Identity = 336/379 (88.65%), Postives = 352/379 (92.88%), Query Frame = 0

Query: 6   MRGCKMGLLIIVGLILMECLLSNNGVDGATANNNKKAVGASFIFGDSLVDAGNNNYLPTL 65
           M GCKM    IVGLILM CLL NNGVDGA   N K  VGASFIFGDSLVDAGNNNYL TL
Sbjct: 4   MGGCKM----IVGLILMGCLLVNNGVDGA---NKKVDVGASFIFGDSLVDAGNNNYLSTL 63

Query: 66  SKANLRPNGMDYKPSGGKPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV 125
           SKANLRPNG+DY  SGG PTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV
Sbjct: 64  SKANLRPNGIDYTLSGGNPTGRFTNGRTIGDIVGEELGIPNHAVPFLDPNATGKSILYGV 123

Query: 126 NYASGGGGILNATGRIFVNRLGMDVQVDFFNVTRKQFDKIMGAEKAKEYIGKKSIFSITI 185
           NYASGGGGILNATGRIFVNRLGMDVQVDFFN+TRKQFDKI+G  KA++YI KKSI SITI
Sbjct: 124 NYASGGGGILNATGRIFVNRLGMDVQVDFFNITRKQFDKILGPSKARDYISKKSIISITI 183

Query: 186 GANDFLNNYLLPVLSVGARISQTPDAFVDDMISHLKNQLTRLYKMDGRKFVVGNVGPIGC 245
           GANDFLNNYLLPVLS+GARISQ+PD+F+DDMI+HLKNQLTRLY MD RKFVVGNVGPIGC
Sbjct: 184 GANDFLNNYLLPVLSIGARISQSPDSFIDDMIAHLKNQLTRLYNMDARKFVVGNVGPIGC 243

Query: 246 IPYQKTINQLNEDECVDLANKLALQYNAKLKDLLSSLNKDLPSSTFVYANVYDLVMDLIV 305
           IPYQKTINQLNED+CVDLANKLALQYNAKLKDLL+ LNK LP+STFVYANVYDLVMDLIV
Sbjct: 244 IPYQKTINQLNEDQCVDLANKLALQYNAKLKDLLADLNKKLPASTFVYANVYDLVMDLIV 303

Query: 306 NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCSERSRHVFWDPYHPSEAANLLIAKKL 365
           NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLC+ERSRHVFWDPYHPSEAANL+IAKKL
Sbjct: 304 NYDNYGFKTASRACCGNGGQFAGIIPCGPQSSLCTERSRHVFWDPYHPSEAANLIIAKKL 363

Query: 366 LDGDHKFISPYNLRQLRDL 384
           LDGDHK+I P NLRQLRDL
Sbjct: 364 LDGDHKYIFPLNLRQLRDL 375