Cucsat.G15096 (gene) Cucumber (B10) v3

BLAST of Cucsat.G15096 vs. ExPASy Swiss-Prot
Match: P55937 (Golgin subfamily A member 3 OS=Mus musculus OX=10090 GN=Golga3 PE=1 SV=3)

HSP 1 Score: 56.6 bits (135), Expect = 1.7e-06
Identity = 139/633 (21.96%), Postives = 281/633 (44.39%), Query Frame = 0

Query: 329  ELQKRAKEAEERVMFL-SEELEQERFNQYRKFDVSDLIQIIRILTGERFTLALEISNLLQ 388
            ELQ+ A   E+ + FL +E++  E   Q  K D  +L +  R L  +    +  +  L Q
Sbjct: 752  ELQREADSREDAIHFLQNEKIVLEVALQSAKSDKEELDRGARRLEEDTEETSGLLEQLRQ 811

Query: 389  SRIADRTCAREELRQANAELESRTQKLEKEKIELQVGLEK-ELDRRSNDW---SFKLEKY 448
              +A ++   E L+Q  A L  + QK++++ ++ +V +E    D  S D      K  K 
Sbjct: 812  D-LAVKSNQVEHLQQETATLRKQMQKVKEQFVQQKVMVEAYRRDATSKDQLINELKATKK 871

Query: 449  QLEEE---------GLRGRVRELAEQNVSLQREVSSLNKM--ETENRTITTNLEQNIMDL 508
            +L+ E          L+G  + +  ++  LQ+++S +++   E E    +   E++ M++
Sbjct: 872  RLDSEMKELRQELIKLQGEKKTVEVEHSRLQKDMSLVHQQMAELEGHLQSVQKERDEMEI 931

Query: 509  ---TAKIDEK-----NEENKYLQLNLSKLEEDYRGAI----EGMDCIRKNYEEKEKECKD 568
               + K D++      E N+ L+  + +L+++ + AI    + M  +  +    +KE K 
Sbjct: 932  HLQSLKFDKEQMIALTEANETLKKQIEELQQEAKKAITEQKQKMKRLGSDLTSAQKEMKT 991

Query: 569  LHK----SITRLSRTCNE---QEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRL 628
             HK    +++ LSR   E    ++  D    +L  Q +         ++   L++E   +
Sbjct: 992  KHKAYENAVSILSRRLQEALASKEATDAELNQLRAQSTGGSSDPVLHEKIRALEVELQNV 1051

Query: 629  TGVELALRKELESYRVEVDSLRHENIKILTRLKDNGNESGAITFKL------DNEMSARV 688
               ++ L KEL+           E+ + +  L+D   ES     K+      + +++  +
Sbjct: 1052 GQSKILLEKELQEVITMTSQELEESREKVLELEDELQESRGFRRKIKRLEESNKKLALEL 1111

Query: 689  YHLQNQ--GLVLLNESTQFCSKLLEFIKEKIDQFHPTEHRMEHIKNGLDGQFFLE-SEMK 748
             H + +  GL   N + +  + +LE    K           + ++  L  Q  L+  E +
Sbjct: 1112 EHERGKLTGLGQSNAALREHNSILETALAK--------READLVQLNLQVQAVLQRKEEE 1171

Query: 749  IRSLKHGIESLTMSLQKISMLLQAKSNPTSQTSDVDNALQLNCQYLEDG-LRSELKAETL 808
             R +K  +++L +SL+K  M + +               Q+    +E G  R   KA TL
Sbjct: 1172 DRQMKQLVQALQVSLEKEKMEVNSLKE------------QMAAARIEAGHNRRHFKAATL 1231

Query: 809  FSSLLREKLYSKE---------------------LEVEQLQTELVTAVRGNDILKCEVQN 868
              S ++++L +KE                      E+ Q QTEL  A     +L+ ++  
Sbjct: 1232 ELSEVKKELQAKEHLVQTLQAEVDELQIQDGKHSQEIAQFQTELAEARTQLQLLQKKLDE 1291

Query: 869  GMDGLSCLTHKMKDLELQLRMKNEEISKLQKGV----EESTRELESVKEVLEKISKERDM 892
             M      + +M+DL+ +L  K  EI  L++ +    ++  +ELE  ++ L+ I  E +M
Sbjct: 1292 QMSQQPTGSQEMEDLKWELDQKEREIQSLKQQLDLTEQQGKKELEGTQQTLQTIKSELEM 1351

BLAST of Cucsat.G15096 vs. ExPASy Swiss-Prot
Match: Q54G05 (Putative leucine-rich repeat-containing protein DDB_G0290503 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0290503 PE=4 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 5.4e-05
Identity = 126/620 (20.32%), Postives = 262/620 (42.26%), Query Frame = 0

Query: 323  NEDFDGELQKRAKEAEERVMFLSEELEQERFNQYRKF-----DVSDLIQIIRILTGERFT 382
            N+    ELQ    E + ++  L E  +        K      ++ D  + +R L     T
Sbjct: 628  NQSIINELQSNLNENQNKINELIENNQSSSDELNSKLIKLSDELKDKNENVRSLE----T 687

Query: 383  LALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIELQVGLEKELDRRSNDWS 442
              +E  + L   I        EL+    E E    +L +        L+ +L+ + N+ +
Sbjct: 688  SIIENQDKLDQLIQSNQVTVNELQSKLNEKEININQLIENNQSSLDELQSKLNEKQNEIN 747

Query: 443  FKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETENRTITTNLEQNIMDLTAKID 502
              +E  Q   + L+ ++ E  ++   LQ   S LN++   N + +  L+  ++ L+ ++ 
Sbjct: 748  QLIENNQSSSDELQSKLNEKHQEISELQ---SKLNELIENNESSSDELQSKLIQLSDELK 807

Query: 503  EKNEENKYLQL----NLSKLEEDYRGAIEGMDCIRKNYEEKEKECKDL----HKSITRLS 562
            EK+E+ K L      N  KL +  +   + +D ++    EK+ E  +L      S   L 
Sbjct: 808  EKDEKLKSLDSIIIENQEKLVQLTKSNQDSLDELQSKLNEKQNEINELIENNQSSSNELQ 867

Query: 563  RTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVELALRKELESYRV 622
               NE++  I+ L E  + Q S+ +   K +++ + +   Q +L   +  + + +E+   
Sbjct: 868  SKLNEKQNEINLLIE--NNQSSSDELQSKLNEKHQEINELQSKLNEKQNKINELVENNES 927

Query: 623  EVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNESTQFCSKLL 682
              D L+ + I++  +L++  N+  +    +  E   ++  LQ++    LNE      ++ 
Sbjct: 928  SSDELQSKLIQLSDQLQEKENQLKSFESSI-IERDEKLNQLQSK----LNEKQNEIDQIT 987

Query: 683  EFIKEKIDQFHPTEHRMEH-----IKNGLDGQFFLESEM-----KIRSLKHGIESLTMSL 742
            E  +  +D+     +  ++     I+N       L+S++     +I    + I  L  + 
Sbjct: 988  ENNQSSLDELQSNLNEKQNEINQLIENNQSSLDELQSKLNEKLNEINEKDNKINELIQTN 1047

Query: 743  QKISMLLQAKSNPTSQTSDVDN--ALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKEL 802
            + +S   Q+K     Q  +  N   L LN Q ++   +   K   L  + L+ KL  K+ 
Sbjct: 1048 ESLSKDQQSKFENLEQELEEKNNKILDLNSQIIDVNHQFSEKENEL--NQLQLKLIEKDQ 1107

Query: 803  EVEQLQTELVTAVRGNDILKCEVQNGMDG----------LSCLTHKMKDLELQLRMKNEE 862
            E+E    +++      +  + E+    D           +  L  K++DLE +L ++ + 
Sbjct: 1108 EIENQNNKIIDINNQLNEKEKEININNDNDNNNEENIQLIEELKEKLQDLENELNLEKDT 1167

Query: 863  ISKLQKGVEESTRELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETL 900
            +++    + E     E +K + EK+S++   + E +N Y E    +N + D++KS  E L
Sbjct: 1168 VNEKNDDINELK---EEIKLISEKLSEKEQELNEMINDYDESLNEINDQKDLVKSLNERL 1227

BLAST of Cucsat.G15096 vs. ExPASy Swiss-Prot
Match: Q63644 (Rho-associated protein kinase 1 OS=Rattus norvegicus OX=10116 GN=Rock1 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 5.4e-05
Identity = 185/782 (23.66%), Postives = 328/782 (41.94%), Query Frame = 0

Query: 218  SREAKGSISRLLSEEVGEYGFGNDSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVT 277
            S+EAK  I   L++   E   G +    I + +  K  Q           L + V   V 
Sbjct: 306  SKEAKNLICAFLTDR--EVRLGRNGVEEIKRHLFFKNDQW------AWETLRDTVAPVVP 365

Query: 278  DIHTRSSNECFDP-NSDLGNQPCFP-------TDAPWKTVSGHMYETYKP---------- 337
            D+ +      FD    D G++  FP          P+   + +    Y P          
Sbjct: 366  DLSSDIDTSNFDDLEEDKGDEETFPIPKAFVGNQLPFVGFTYYSNRRYLPSANPSENRSS 425

Query: 338  GETNEDFDGELQKRAKEAEERV---MFLSEELEQERFNQYRKFDVSDLIQIIRILTGERF 397
               +++    LQK   + EE++   M L +E+EQ+      K D   +++ +     +R 
Sbjct: 426  SNVDKNVQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLD--KIMKELDEEGNQRR 485

Query: 398  TLALEISN------LLQSRIAD--RTCAREELRQANAELE--------------SRTQKL 457
             L   +S       LLQ RI +  R   +E  ++ N E E              S++ +L
Sbjct: 486  NLESAVSQIEKEKMLLQHRINEYQRKVEQENEKRRNVENEVSTLKDQLEDLRKASQSSQL 545

Query: 458  EKEKI-ELQVGLEK--ELDRRSNDWSFKLEKYQLE----EEGLRGRVRELAEQNVSLQRE 517
              EK+ +LQ  LE+  +L R  +D + +L K   E       L    REL E+N  L+  
Sbjct: 546  ANEKLTQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSVSQLESLNRELQERNRMLENS 605

Query: 518  VSSLNK--------METENRTITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRG 577
             S  +K        +E E R    + E  I DL A+I    EE K+L+ NL ++E + + 
Sbjct: 606  KSQADKDYYQLQAVLEAERRDRGHDSEM-IGDLQARITSLQEEVKHLKHNLERVEGERK- 665

Query: 578  AIEGMDCIRKNYEEKEKECKDLH-----KSI-TRLSRTCNEQEKTIDGLRERLSEQFSNI 637
              E  D +  N+ EKEK   ++      KSI  RL +  NE + T    + RL+++  +I
Sbjct: 666  --EAQDML--NHSEKEKNNLEIDLNYKLKSIQQRLEQEVNEHKVT----KARLTDKHQSI 725

Query: 638  QPVEKFDKQCE---RLKME-QMRLTGVELALRKELESYRVEVD-SLRHENIKILTRLKDN 697
            +   K    CE   +LK E + R       +  E +   ++VD     + ++ LT  K+ 
Sbjct: 726  EEA-KSVAMCEMEKKLKEEREAREKAENRVVETEKQCSMLDVDLKQSQQKLEHLTENKER 785

Query: 698  GNES-GAITFKLDNEMSARVY---HLQNQGLVLLN------------ESTQFCSKLLEF- 757
              ++  ++T +L+ E + R+     L+ Q     N             +     +LLEF 
Sbjct: 786  LEDAVKSLTLQLEQESNKRILLQSELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFE 845

Query: 758  IKEKIDQFHPTEHRMEHIKNGLDGQFFLES--EMKIRSLKHGIESLTM-SLQKISMLLQA 817
            + +   Q+   E +M  +++ L+ + +  +  + +++ LK  IE     +L+KI  L   
Sbjct: 846  LAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLRKIQELQSE 905

Query: 818  KSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKELEVEQLQTELVT 877
            K   ++Q                D   ++ ++E L   +L E+ +    E ++  +    
Sbjct: 906  KETLSTQL---------------DLAETKAESEQLARGILEEQYFELTQESKKAASRNRQ 965

Query: 878  AVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEESTR-----ELE 905
             +   D     ++   + L+      KD+EL LR +NEE+++  +  EE  +     E+ 
Sbjct: 966  EITDKDHTVSRLEEANNALT------KDIEL-LRKENEELNERMRTAEEEYKLKKEEEIS 1025

BLAST of Cucsat.G15096 vs. ExPASy Swiss-Prot
Match: P25386 (Intracellular protein transport protein USO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=USO1 PE=1 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 7.1e-05
Identity = 105/489 (21.47%), Postives = 217/489 (44.38%), Query Frame = 0

Query: 454  VRELAEQNVSLQREVSSLNKMETENRTITTNLEQNIMDLT---AKIDEK----NEENKYL 513
            V +L  Q   L+ E++SL   +TE  +   NL + ++ LT    ++DEK    N  +  L
Sbjct: 732  VEKLQRQCTKLKGEITSL---QTETESTHENLTEKLIALTNEHKELDEKYQILNSSHSSL 791

Query: 514  QLNLSKLEEDYRGAIEGMDCIRKNYEEKEKECKDLHKSITRLSRTCNEQEKTI----DGL 573
            + N S LE + +   + +D + +  +  E + K+   ++     T ++QE +I     GL
Sbjct: 792  KENFSILETELKNVRDSLDEMTQLRDVLETKDKENQTALLEYKSTIHKQEDSIKTLEKGL 851

Query: 574  RERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVELALRKELE----SYRVEVDSLRHEN 633
               LS++      + K  K    L  E   +      L+KE +    +++ E  SL+ + 
Sbjct: 852  ETILSQKKKAEDGINKMGKDLFALSREMQAVEENCKNLQKEKDKSNVNHQKETKSLKEDI 911

Query: 634  IKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNESTQFCS------KLLEFI 693
               +T +K        +  + +N +S    H+  +   L+   ++F S      KL E +
Sbjct: 912  AAKITEIKAINENLEEMKIQCNN-LSKEKEHISKE---LVEYKSRFQSHDNLVAKLTEKL 971

Query: 694  KEKIDQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTMSLQKISMLLQAKSNP 753
            K   + +   +   E +   ++ +   ES +++ +L++ I+S++   +   +   +    
Sbjct: 972  KSLANNYKDMQAENESLIKAVE-ESKNESSIQLSNLQNKIDSMSQEKENFQIERGSIEKN 1031

Query: 754  TSQTSDVDNALQLNCQYL---EDGLRSELKAETLFSSLLREKL----------YSKELEV 813
              Q     + L+   + +    D  + E +++    SLL+EKL           +K  E+
Sbjct: 1032 IEQLKKTISDLEQTKEEIISKSDSSKDEYESQI---SLLKEKLETATTANDENVNKISEL 1091

Query: 814  EQLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEEST 873
             + + EL   +     LK E++  ++       ++K+ E  L+   EE  +L+K   E+ 
Sbjct: 1092 TKTREELEAELAAYKNLKNELETKLETSEKALKEVKENEEHLK---EEKIQLEKEATETK 1151

Query: 874  RELESVKEVLEKISKERDMMLEEVNKYRE----KNMLLNSEVDVLKSNIETLEEDNLLKE 905
            ++L S++  LE + KE + +  ++ KY E    K    N E+  L   I + +++N  + 
Sbjct: 1152 QQLNSLRANLESLEKEHEDLAAQLKKYEEQIANKERQYNEEISQLNDEITSTQQEN--ES 1204

BLAST of Cucsat.G15096 vs. ExPASy Swiss-Prot
Match: P70335 (Rho-associated protein kinase 1 OS=Mus musculus OX=10090 GN=Rock1 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.7e-04
Identity = 190/785 (24.20%), Postives = 328/785 (41.78%), Query Frame = 0

Query: 218  SREAKGSISRLLSEEVGEYGFGNDSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVT 277
            S+EAK  I   L++   E   G +    I + +  K  Q           L + V   V 
Sbjct: 306  SKEAKNLICAFLTDR--EVRLGRNGVEEIKRHLFFKNDQW------AWETLRDTVAPVVP 365

Query: 278  DIHTRSSNECFDP-NSDLGNQPCFP-------TDAPWKTVSGHMYETYKP---------- 337
            D+ +      FD    D G++  FP          P+   + +    Y P          
Sbjct: 366  DLSSDIDTSNFDDLEEDKGDEETFPIPKAFVGNQLPFVGFTYYSNRRYLPSANASENRSS 425

Query: 338  GETNEDFDGELQKRAKEAEERV---MFLSEELEQERFNQYRKFDVSDLIQIIRILTGERF 397
               ++     LQK   + EE++   M L +E+EQ+      K D   +++ +     +R 
Sbjct: 426  SNVDKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNLKLD--KIMKELDEEGNQRR 485

Query: 398  TLALEISN------LLQSRIAD--RTCAREELRQANAELE--------------SRTQKL 457
             L   +S       LLQ RI +  R   +E  ++ N E E              S+T +L
Sbjct: 486  NLESAVSQIEKEKMLLQHRINEYQRKVEQENEKRRNIENEVSTLKDQLEDLRKASQTSQL 545

Query: 458  EKEKI-ELQVGLEK--ELDRRSNDWSFKLEKYQLEE----EGLRGRVRELAEQNVSLQRE 517
              EK+ +LQ  LE+  +L R  +D + +L K   E       L    REL E+N  L+  
Sbjct: 546  ANEKLTQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENS 605

Query: 518  VSSLNK--------METENRTITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRG 577
             S  +K        +E E R    + E  I DL A+I    EE K+L+ NL ++E + + 
Sbjct: 606  KSQADKDYYQLQAVLEAERRDRGHDSEM-IGDLQARITSLQEEVKHLKHNLERVEGERK- 665

Query: 578  AIEGMDCIRKNYEEKEKECKDLH-----KSI-TRLSRTCNEQEKTIDGLRERLSEQFSNI 637
              E  D +  N+ EKEK   ++      KSI  RL +  NE + T    + RL+++  +I
Sbjct: 666  --EAQDML--NHSEKEKNNLEIDLNYKLKSIQQRLEQEVNEHKVT----KARLTDKHQSI 725

Query: 638  QPVEKFDKQCE---RLKME-QMRLTGVELALRKELESYRVEVD-SLRHENIKILTRLKDN 697
            +   K    CE   +LK E + R       +  E +   ++VD     + ++ LT  K+ 
Sbjct: 726  EEA-KSVAMCEMEKKLKEEREAREKAENRVVETEKQCSMLDVDLKQSQQKLEHLTENKER 785

Query: 698  -GNESGAITFKLDNEMSARVYHLQNQGLVLLNESTQF----------------CSKLLEF 757
              +E   +  +L+ E + R+  LQN+      E+                     +LLEF
Sbjct: 786  MEDEVKNLALQLEQESNKRLL-LQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEF 845

Query: 758  -IKEKIDQFHPTEHRMEHIKNGLDGQFFLES--EMKIRSLKHGIESLTM-SLQKISMLLQ 817
             + +   Q+   E +M  +++ L+ + +  +  + +++ LK  IE     +L+KI  L  
Sbjct: 846  ELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLRKIQELQS 905

Query: 818  AKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKELEVEQLQTELV 877
             K   ++Q                D   ++ ++E L   +L E+ +    E ++  +   
Sbjct: 906  EKETLSTQL---------------DLAETKAESEQLARGILEEQYFELTQESKKAASRNR 965

Query: 878  TAVRGND--ILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEESTR----- 905
              +   D  + + E  N     S LT   KD+E+ LR +NEE+++  +  EE  +     
Sbjct: 966  QEITDKDHTVSRLEETN-----SVLT---KDIEM-LRKENEELNERMRTAEEEYKLKKEE 1025

BLAST of Cucsat.G15096 vs. NCBI nr
Match: XP_011654928.1 (golgin subfamily A member 6-like protein 6 [Cucumis sativus] >XP_031741443.1 golgin subfamily A member 6-like protein 6 [Cucumis sativus] >KGN50489.1 hypothetical protein Csa_000087 [Cucumis sativus])

HSP 1 Score: 1697 bits (4395), Expect = 0.0
Identity = 893/895 (99.78%), Postives = 893/895 (99.78%), Query Frame = 0

Query: 1   MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSSKAGSSPQSTRSGKHIDDSE 60
           MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSSKAGSSPQSTRSGKHIDDSE
Sbjct: 1   MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSSKAGSSPQSTRSGKHIDDSE 60

Query: 61  RFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSSRCQSPSRE 120
           RFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSSRCQSPSRE
Sbjct: 61  RFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSSRCQSPSRE 120

Query: 121 MQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING 180
           MQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING
Sbjct: 121 MQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING 180

Query: 181 SMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEEVGEYGFGN 240
           SMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEEVGEYGFGN
Sbjct: 181 SMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEEVGEYGFGN 240

Query: 241 DSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNSDLGNQPCF 300
           DSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNSDLGNQPCF
Sbjct: 241 DSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNSDLGNQPCF 300

Query: 301 PTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQERFNQYRKFD 360
           PTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQERFNQYRKFD
Sbjct: 301 PTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQERFNQYRKFD 360

Query: 361 VSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE 420
           VSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE
Sbjct: 361 VSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE 420

Query: 421 LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETENRT 480
           LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETENRT
Sbjct: 421 LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETENRT 480

Query: 481 ITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD 540
           ITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD
Sbjct: 481 ITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD 540

Query: 541 LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVELAL 600
           LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVELAL
Sbjct: 541 LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVELAL 600

Query: 601 RKELESYRVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE 660
           RKELES RVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE
Sbjct: 601 RKELESCRVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE 660

Query: 661 STQFCSKLLEFIKEKIDQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTMSLQ 720
           STQFCSKLLEFIKEKI QFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTMSLQ
Sbjct: 661 STQFCSKLLEFIKEKIGQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTMSLQ 720

Query: 721 KISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKELEVE 780
           KISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKELEVE
Sbjct: 721 KISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKELEVE 780

Query: 781 QLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEESTR 840
           QLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEESTR
Sbjct: 781 QLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEESTR 840

Query: 841 ELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETLEEDNLLKE 895
           ELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETLEEDNLLKE
Sbjct: 841 ELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETLEEDNLLKE 895

BLAST of Cucsat.G15096 vs. NCBI nr
Match: XP_008445818.1 (PREDICTED: rho-associated protein kinase 1 [Cucumis melo] >XP_008445819.1 PREDICTED: rho-associated protein kinase 1 [Cucumis melo] >KAA0034026.1 rho-associated protein kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1591 bits (4120), Expect = 0.0
Identity = 839/895 (93.74%), Postives = 863/895 (96.42%), Query Frame = 0

Query: 1   MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSSKAGSSPQSTRSGKHIDDSE 60
           MKKLFLRSFGTGHGKNNSA+PSTNES+THWE+P ESRTSSSKAGSSPQSTRSGKHIDDSE
Sbjct: 1   MKKLFLRSFGTGHGKNNSALPSTNESQTHWEHPFESRTSSSKAGSSPQSTRSGKHIDDSE 60

Query: 61  RFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSSRCQSPSRE 120
           RFG GPKLRRTRSLSSAAFRDQGQ+DFYGSSDPSR+PGNSSSGFK+Q EPSS CQSPSRE
Sbjct: 61  RFGMGPKLRRTRSLSSAAFRDQGQVDFYGSSDPSRTPGNSSSGFKQQCEPSSCCQSPSRE 120

Query: 121 MQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING 180
           MQF  KQMEMPNDYY SG +RPSSR CYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING
Sbjct: 121 MQFKTKQMEMPNDYYTSGFVRPSSRACYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING 180

Query: 181 SMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEEVGEYGFGN 240
           SM+KCSQR+NGWRPPRAQCLP+TSTTASIKDKPRSYSSREAKGSIS LLSEEVGEYGFGN
Sbjct: 181 SMNKCSQRNNGWRPPRAQCLPHTSTTASIKDKPRSYSSREAKGSISHLLSEEVGEYGFGN 240

Query: 241 DSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNSDLGNQPCF 300
           DSP+SIAKTVVD+LSQHHVVPKA SRELGENVPITVTDIHTRSSN CFDPNSDL NQPCF
Sbjct: 241 DSPQSIAKTVVDRLSQHHVVPKANSRELGENVPITVTDIHTRSSNGCFDPNSDLANQPCF 300

Query: 301 PTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQERFNQYRKFD 360
           PTDAPWKTVS HMYET KP ETNEDFDGELQKRAKEAEERVM+LSEELEQERFNQYRKFD
Sbjct: 301 PTDAPWKTVSEHMYETCKPSETNEDFDGELQKRAKEAEERVMYLSEELEQERFNQYRKFD 360

Query: 361 VSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE 420
           VSDLIQII+ILTGERFT ALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE
Sbjct: 361 VSDLIQIIKILTGERFTFALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE 420

Query: 421 LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETENRT 480
           LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKM TENRT
Sbjct: 421 LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMVTENRT 480

Query: 481 ITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD 540
           ITTNLEQNI+DLTAKIDEKNEENKYLQ+NLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD
Sbjct: 481 ITTNLEQNIVDLTAKIDEKNEENKYLQINLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD 540

Query: 541 LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVELAL 600
           LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQ ERLK+EQMRLTGVELAL
Sbjct: 541 LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQFERLKIEQMRLTGVELAL 600

Query: 601 RKELESYRVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE 660
           RKELES RVEVDSLR ENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE
Sbjct: 601 RKELESCRVEVDSLRRENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE 660

Query: 661 STQFCSKLLEFIKEKIDQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTMSLQ 720
           STQFCSKLLEFIKEK+ QF PTEHRMEHIKNGLDGQFF+ESE+KI+SLKHGIESLTMSLQ
Sbjct: 661 STQFCSKLLEFIKEKVGQFCPTEHRMEHIKNGLDGQFFIESEIKIQSLKHGIESLTMSLQ 720

Query: 721 KISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKELEVE 780
           KISMLLQAKSNPTSQTS VD  LQLNCQY EDGLRSELKAETLFSSLLREKLYSKELEVE
Sbjct: 721 KISMLLQAKSNPTSQTSGVDT-LQLNCQYPEDGLRSELKAETLFSSLLREKLYSKELEVE 780

Query: 781 QLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEESTR 840
           QLQ ELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKL  GVEESTR
Sbjct: 781 QLQAELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLHMGVEESTR 840

Query: 841 ELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETLEEDNLLKE 895
           ELESVK +LEK+SKERDMMLEEVNKYREKNMLLNSEVDVLKSN+ETLEED LLKE
Sbjct: 841 ELESVKAILEKVSKERDMMLEEVNKYREKNMLLNSEVDVLKSNVETLEEDILLKE 894

BLAST of Cucsat.G15096 vs. NCBI nr
Match: XP_038893371.1 (rho-associated protein kinase 1 isoform X1 [Benincasa hispida] >XP_038893372.1 rho-associated protein kinase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1508 bits (3904), Expect = 0.0
Identity = 799/898 (88.98%), Postives = 844/898 (93.99%), Query Frame = 0

Query: 1   MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSS---KAGSSPQSTRSGKHID 60
           MKKLF RSFGTG+GK+N A+PSTNESETH E+PLE R SSS   KAGSSPQSTRSGK ID
Sbjct: 1   MKKLFFRSFGTGNGKHNLALPSTNESETHLEHPLEGRKSSSISDKAGSSPQSTRSGKQID 60

Query: 61  DSERFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSSRCQSP 120
           DSER  TGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGN+SSGFKRQHE SSRCQSP
Sbjct: 61  DSERSSTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNASSGFKRQHEQSSRCQSP 120

Query: 121 SREMQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDGEQHQE 180
           SREMQF AKQ+EMP+DYY SG +RP SRTCYDSSGNSS SVSSVSNRVLDRYIDGEQHQE
Sbjct: 121 SREMQFKAKQLEMPHDYYTSGPVRPCSRTCYDSSGNSSNSVSSVSNRVLDRYIDGEQHQE 180

Query: 181 INGSMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEEVGEYG 240
           INGSM+KC QR+NGWRPPRAQCL + ST+ASIKDKPRSYSSREAK S SRLLS EV EYG
Sbjct: 181 INGSMNKCFQRNNGWRPPRAQCLLHASTSASIKDKPRSYSSREAKSSHSRLLSGEVVEYG 240

Query: 241 FGNDSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNSDLGNQ 300
           FGNDSPRSIAK VVD+LSQHHVVPKATS+EL EN+PITVTDIH+RSSN CFDPNSDL  Q
Sbjct: 241 FGNDSPRSIAKNVVDRLSQHHVVPKATSKELDENIPITVTDIHSRSSNGCFDPNSDLATQ 300

Query: 301 PCFPTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQERFNQYR 360
           PCFPTD PW+TVSGH+YE+ KPGETNEDFDGELQKRAKEAEERV+FLSEELEQERFNQYR
Sbjct: 301 PCFPTDEPWETVSGHIYESCKPGETNEDFDGELQKRAKEAEERVVFLSEELEQERFNQYR 360

Query: 361 KFDVSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKE 420
           KFDVSDLIQII+ L GERFTLALEISNLLQSRIADRTCAR+ELRQANAELESRT KLEKE
Sbjct: 361 KFDVSDLIQIIKNLNGERFTLALEISNLLQSRIADRTCARKELRQANAELESRTLKLEKE 420

Query: 421 KIELQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETE 480
           KIELQVGLEKELDRRS+DWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETE
Sbjct: 421 KIELQVGLEKELDRRSSDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETE 480

Query: 481 NRTITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNYEEKEKE 540
           NR+ITTNLEQNI+DLTA+IDEKNE+NKYLQLNLSKLEEDYRGAIEGMDCIRKN+EEKEKE
Sbjct: 481 NRSITTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRGAIEGMDCIRKNFEEKEKE 540

Query: 541 CKDLHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVE 600
           C +LHKSITRL RTCNEQEKTIDGLRERLSEQF NIQPVEK DKQ ERLKMEQMRLTGVE
Sbjct: 541 CGELHKSITRLLRTCNEQEKTIDGLRERLSEQFGNIQPVEKLDKQFERLKMEQMRLTGVE 600

Query: 601 LALRKELESYRVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVL 660
           LALRKELESYRVEVDSLRHENIKILTRLK+NGNESGAITFKLDNEMSARVYHLQNQGLVL
Sbjct: 601 LALRKELESYRVEVDSLRHENIKILTRLKENGNESGAITFKLDNEMSARVYHLQNQGLVL 660

Query: 661 LNESTQFCSKLLEFIKEKIDQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTM 720
           LNESTQFCSKLLEFIKEK+ QFHPT+HR+E+IKNGL GQFFLESEMKIRS KHGIESLTM
Sbjct: 661 LNESTQFCSKLLEFIKEKVGQFHPTKHRIEYIKNGLYGQFFLESEMKIRSFKHGIESLTM 720

Query: 721 SLQKISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKEL 780
           SLQKISMLLQAKSN TSQ+S VDNALQL+CQY EDGLRSELKAETLFSSLLREKLYSKEL
Sbjct: 721 SLQKISMLLQAKSNSTSQSSGVDNALQLSCQYAEDGLRSELKAETLFSSLLREKLYSKEL 780

Query: 781 EVEQLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEE 840
           E+EQLQ ELVTAVRGNDILKCEVQNGMD LSCLTHKMKDLELQL  KNE+I+KL  G+EE
Sbjct: 781 EMEQLQAELVTAVRGNDILKCEVQNGMDSLSCLTHKMKDLELQLLKKNEDINKLHNGLEE 840

Query: 841 STRELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETLEEDNLLKE 895
           STRELE++K++LEKISKERDMMLEEVNK REKNMLLNSEVD+LKS IETLEED LLKE
Sbjct: 841 STRELETLKDILEKISKERDMMLEEVNKNREKNMLLNSEVDMLKSKIETLEEDILLKE 898

BLAST of Cucsat.G15096 vs. NCBI nr
Match: XP_038893373.1 (protein Daple isoform X2 [Benincasa hispida])

HSP 1 Score: 1408 bits (3644), Expect = 0.0
Identity = 739/826 (89.47%), Postives = 777/826 (94.07%), Query Frame = 0

Query: 1   MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSS---KAGSSPQSTRSGKHID 60
           MKKLF RSFGTG+GK+N A+PSTNESETH E+PLE R SSS   KAGSSPQSTRSGK ID
Sbjct: 1   MKKLFFRSFGTGNGKHNLALPSTNESETHLEHPLEGRKSSSISDKAGSSPQSTRSGKQID 60

Query: 61  DSERFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSSRCQSP 120
           DSER  TGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGN+SSGFKRQHE SSRCQSP
Sbjct: 61  DSERSSTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNASSGFKRQHEQSSRCQSP 120

Query: 121 SREMQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDGEQHQE 180
           SREMQF AKQ+EMP+DYY SG +RP SRTCYDSSGNSS SVSSVSNRVLDRYIDGEQHQE
Sbjct: 121 SREMQFKAKQLEMPHDYYTSGPVRPCSRTCYDSSGNSSNSVSSVSNRVLDRYIDGEQHQE 180

Query: 181 INGSMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEEVGEYG 240
           INGSM+KC QR+NGWRPPRAQCL + ST+ASIKDKPRSYSSREAK S SRLLS EV EYG
Sbjct: 181 INGSMNKCFQRNNGWRPPRAQCLLHASTSASIKDKPRSYSSREAKSSHSRLLSGEVVEYG 240

Query: 241 FGNDSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNSDLGNQ 300
           FGNDSPRSIAK VVD+LSQHHVVPKATS+EL EN+PITVTDIH+RSSN CFDPNSDL  Q
Sbjct: 241 FGNDSPRSIAKNVVDRLSQHHVVPKATSKELDENIPITVTDIHSRSSNGCFDPNSDLATQ 300

Query: 301 PCFPTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQERFNQYR 360
           PCFPTD PW+TVSGH+YE+ KPGETNEDFDGELQKRAKEAEERV+FLSEELEQERFNQYR
Sbjct: 301 PCFPTDEPWETVSGHIYESCKPGETNEDFDGELQKRAKEAEERVVFLSEELEQERFNQYR 360

Query: 361 KFDVSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKE 420
           KFDVSDLIQII+ L GERFTLALEISNLLQSRIADRTCAR+ELRQANAELESRT KLEKE
Sbjct: 361 KFDVSDLIQIIKNLNGERFTLALEISNLLQSRIADRTCARKELRQANAELESRTLKLEKE 420

Query: 421 KIELQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETE 480
           KIELQVGLEKELDRRS+DWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETE
Sbjct: 421 KIELQVGLEKELDRRSSDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETE 480

Query: 481 NRTITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNYEEKEKE 540
           NR+ITTNLEQNI+DLTA+IDEKNE+NKYLQLNLSKLEEDYRGAIEGMDCIRKN+EEKEKE
Sbjct: 481 NRSITTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRGAIEGMDCIRKNFEEKEKE 540

Query: 541 CKDLHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVE 600
           C +LHKSITRL RTCNEQEKTIDGLRERLSEQF NIQPVEK DKQ ERLKMEQMRLTGVE
Sbjct: 541 CGELHKSITRLLRTCNEQEKTIDGLRERLSEQFGNIQPVEKLDKQFERLKMEQMRLTGVE 600

Query: 601 LALRKELESYRVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVL 660
           LALRKELESYRVEVDSLRHENIKILTRLK+NGNESGAITFKLDNEMSARVYHLQNQGLVL
Sbjct: 601 LALRKELESYRVEVDSLRHENIKILTRLKENGNESGAITFKLDNEMSARVYHLQNQGLVL 660

Query: 661 LNESTQFCSKLLEFIKEKIDQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTM 720
           LNESTQFCSKLLEFIKEK+ QFHPT+HR+E+IKNGL GQFFLESEMKIRS KHGIESLTM
Sbjct: 661 LNESTQFCSKLLEFIKEKVGQFHPTKHRIEYIKNGLYGQFFLESEMKIRSFKHGIESLTM 720

Query: 721 SLQKISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKEL 780
           SLQKISMLLQAKSN TSQ+S VDNALQL+CQY EDGLRSELKAETLFSSLLREKLYSKEL
Sbjct: 721 SLQKISMLLQAKSNSTSQSSGVDNALQLSCQYAEDGLRSELKAETLFSSLLREKLYSKEL 780

Query: 781 EVEQLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRM 823
           E+EQLQ ELVTAVRGNDILKCEVQNGMD LSCLTHKMKDLELQ  +
Sbjct: 781 EMEQLQAELVTAVRGNDILKCEVQNGMDSLSCLTHKMKDLELQFSL 826

BLAST of Cucsat.G15096 vs. NCBI nr
Match: XP_022138914.1 (myosin-13 [Momordica charantia] >XP_022138915.1 myosin-13 [Momordica charantia])

HSP 1 Score: 1285 bits (3325), Expect = 0.0
Identity = 697/920 (75.76%), Postives = 782/920 (85.00%), Query Frame = 0

Query: 1   MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSS---KAGSSPQSTR-----S 60
           MKK F RS      K+N A PSTN+ + +WE+PLESR ++S   KAGSSPQST+     S
Sbjct: 1   MKKFFFRS------KHNIAPPSTNDCKAYWEHPLESRMNNSIGDKAGSSPQSTKHFSSKS 60

Query: 61  GKHIDDSERFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSS 120
           G   +D ER  T PKLRRTRSLSSAAF DQGQ++FYG SDPSRSPG+     +RQHE SS
Sbjct: 61  GMQTNDIERSSTSPKLRRTRSLSSAAFGDQGQMNFYGPSDPSRSPGS-----QRQHEQSS 120

Query: 121 RCQSPSREMQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDG 180
           RCQ P+ EMQF  KQME+PNDYY  G +RP S+TCYDSSGNSSTS S+VSNRVLDRYIDG
Sbjct: 121 RCQGPTWEMQFKVKQMEVPNDYYT-GPVRPCSKTCYDSSGNSSTSSSNVSNRVLDRYIDG 180

Query: 181 EQHQEINGSMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEE 240
           EQHQEI+GS +K SQ++NGWRPPRAQCL  +S TASIKDKPRSYSSRE K S SR  S E
Sbjct: 181 EQHQEISGSKNKYSQKNNGWRPPRAQCLTPSSPTASIKDKPRSYSSREVKSSHSRFSSRE 240

Query: 241 VGEYGFGNDSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNS 300
           +GEYGFGN+SPRSIAK VVD+LSQ+HV+PKATS+ELGENVPI  TDI  +S N C+DPN 
Sbjct: 241 LGEYGFGNESPRSIAKNVVDRLSQYHVLPKATSKELGENVPIRTTDIQNQSLNGCYDPNL 300

Query: 301 DLGNQPCFPTDAPWKTVSG-----------------HMYETYKPGETNEDFDGELQKRAK 360
           D+  +PCFPTD P +TVSG                  MYE  K GET+ D DGELQ+  K
Sbjct: 301 DVVTRPCFPTDEPCETVSGPHTEELSGFHKQKISPGRMYEGCKSGETDGDLDGELQRSVK 360

Query: 361 EAEERVMFLSEELEQERFNQYRKFDVSDLIQIIRILTGERFTLALEISNLLQSRIADRTC 420
           EA+ER+MFLSEELEQERF QYRKFDVSDLIQ+I+ L+ +RFTLALEIS+LLQSRIADR  
Sbjct: 361 EADERIMFLSEELEQERFVQYRKFDVSDLIQVIKNLSEDRFTLALEISSLLQSRIADRES 420

Query: 421 AREELRQANAELESRTQKLEKEKIELQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVR 480
           A+EELRQANAEL+SRT+KLEKEK ELQ+GLEKELDRRS+DWSFKLEKY+LEE+GLRGRVR
Sbjct: 421 AKEELRQANAELDSRTRKLEKEKTELQLGLEKELDRRSSDWSFKLEKYKLEEQGLRGRVR 480

Query: 481 ELAEQNVSLQREVSSLNKMETENRTITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEE 540
           ELAEQNVSLQREVSSLNK ETEN++  TNLEQN++DLT +IDEKNE+N YLQLNLSKLEE
Sbjct: 481 ELAEQNVSLQREVSSLNKTETENKSTITNLEQNVLDLTTRIDEKNEQNNYLQLNLSKLEE 540

Query: 541 DYRGAIEGMDCIRKNYEEKEKECKDLHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQP 600
           DYRGA EGMDCIRKN+EEKEKEC++LHKSITRLSRTC+EQEKTIDGLRERLSEQF NIQP
Sbjct: 541 DYRGATEGMDCIRKNFEEKEKECRELHKSITRLSRTCSEQEKTIDGLRERLSEQFVNIQP 600

Query: 601 VEKFDKQCERLKMEQMRLTGVELALRKELESYRVEVDSLRHENIKILTRLKDNGNESGAI 660
           VEKFDK  E+LKMEQMRLTGVE+ALRKELESYRVEVDSLRHENI ILTRLKDNGNESGAI
Sbjct: 601 VEKFDKHFEKLKMEQMRLTGVEMALRKELESYRVEVDSLRHENINILTRLKDNGNESGAI 660

Query: 661 TFKLDNEMSARVYHLQNQGLVLLNESTQFCSKLLEFIKEKIDQFHPTEHRMEHIKNGLDG 720
            FKLDNEMS+RVYHLQNQGLVLL ESTQFCSKLLEFIKEK+ Q   T+HRMEH+KNGLDG
Sbjct: 661 NFKLDNEMSSRVYHLQNQGLVLLKESTQFCSKLLEFIKEKVGQLQQTKHRMEHMKNGLDG 720

Query: 721 QFFLESEMKIRSLKHGIESLTMSLQKISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLR 780
           QFF+ESE KI+  KHGIESLTMSL + SM+LQAKSNPTSQ+S VDNALQ+N QY ED LR
Sbjct: 721 QFFVESETKIQGFKHGIESLTMSLHRTSMVLQAKSNPTSQSSGVDNALQINSQYPEDVLR 780

Query: 781 SELKAETLFSSLLREKLYSKELEVEQLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMK 840
           SELKAETL +SLLREKLYSKELEVEQLQ ELVTAVRGNDILKCEVQN MD LSCLTHKMK
Sbjct: 781 SELKAETLLTSLLREKLYSKELEVEQLQAELVTAVRGNDILKCEVQNQMDSLSCLTHKMK 840

Query: 841 DLELQLRMKNEEISKLQKGVEESTRELESVKEVLEKISKERDMMLEEVNKYREKNMLLNS 895
           DLELQL  KN++I KLQ G EESTRELE+++++LEKISKERDM+ EE NKYRE NMLLNS
Sbjct: 841 DLELQLLKKNDDIFKLQNGFEESTRELETLRDILEKISKERDMVWEEGNKYRENNMLLNS 900

BLAST of Cucsat.G15096 vs. ExPASy TrEMBL
Match: A0A0A0KNP2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G177090 PE=4 SV=1)

HSP 1 Score: 1697 bits (4395), Expect = 0.0
Identity = 893/895 (99.78%), Postives = 893/895 (99.78%), Query Frame = 0

Query: 1   MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSSKAGSSPQSTRSGKHIDDSE 60
           MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSSKAGSSPQSTRSGKHIDDSE
Sbjct: 1   MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSSKAGSSPQSTRSGKHIDDSE 60

Query: 61  RFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSSRCQSPSRE 120
           RFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSSRCQSPSRE
Sbjct: 61  RFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSSRCQSPSRE 120

Query: 121 MQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING 180
           MQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING
Sbjct: 121 MQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING 180

Query: 181 SMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEEVGEYGFGN 240
           SMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEEVGEYGFGN
Sbjct: 181 SMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEEVGEYGFGN 240

Query: 241 DSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNSDLGNQPCF 300
           DSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNSDLGNQPCF
Sbjct: 241 DSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNSDLGNQPCF 300

Query: 301 PTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQERFNQYRKFD 360
           PTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQERFNQYRKFD
Sbjct: 301 PTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQERFNQYRKFD 360

Query: 361 VSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE 420
           VSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE
Sbjct: 361 VSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE 420

Query: 421 LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETENRT 480
           LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETENRT
Sbjct: 421 LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETENRT 480

Query: 481 ITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD 540
           ITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD
Sbjct: 481 ITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD 540

Query: 541 LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVELAL 600
           LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVELAL
Sbjct: 541 LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVELAL 600

Query: 601 RKELESYRVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE 660
           RKELES RVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE
Sbjct: 601 RKELESCRVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE 660

Query: 661 STQFCSKLLEFIKEKIDQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTMSLQ 720
           STQFCSKLLEFIKEKI QFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTMSLQ
Sbjct: 661 STQFCSKLLEFIKEKIGQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTMSLQ 720

Query: 721 KISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKELEVE 780
           KISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKELEVE
Sbjct: 721 KISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKELEVE 780

Query: 781 QLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEESTR 840
           QLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEESTR
Sbjct: 781 QLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEESTR 840

Query: 841 ELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETLEEDNLLKE 895
           ELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETLEEDNLLKE
Sbjct: 841 ELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETLEEDNLLKE 895

BLAST of Cucsat.G15096 vs. ExPASy TrEMBL
Match: A0A1S3BDK7 (rho-associated protein kinase 1 OS=Cucumis melo OX=3656 GN=LOC103488729 PE=4 SV=1)

HSP 1 Score: 1591 bits (4120), Expect = 0.0
Identity = 839/895 (93.74%), Postives = 863/895 (96.42%), Query Frame = 0

Query: 1   MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSSKAGSSPQSTRSGKHIDDSE 60
           MKKLFLRSFGTGHGKNNSA+PSTNES+THWE+P ESRTSSSKAGSSPQSTRSGKHIDDSE
Sbjct: 1   MKKLFLRSFGTGHGKNNSALPSTNESQTHWEHPFESRTSSSKAGSSPQSTRSGKHIDDSE 60

Query: 61  RFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSSRCQSPSRE 120
           RFG GPKLRRTRSLSSAAFRDQGQ+DFYGSSDPSR+PGNSSSGFK+Q EPSS CQSPSRE
Sbjct: 61  RFGMGPKLRRTRSLSSAAFRDQGQVDFYGSSDPSRTPGNSSSGFKQQCEPSSCCQSPSRE 120

Query: 121 MQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING 180
           MQF  KQMEMPNDYY SG +RPSSR CYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING
Sbjct: 121 MQFKTKQMEMPNDYYTSGFVRPSSRACYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING 180

Query: 181 SMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEEVGEYGFGN 240
           SM+KCSQR+NGWRPPRAQCLP+TSTTASIKDKPRSYSSREAKGSIS LLSEEVGEYGFGN
Sbjct: 181 SMNKCSQRNNGWRPPRAQCLPHTSTTASIKDKPRSYSSREAKGSISHLLSEEVGEYGFGN 240

Query: 241 DSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNSDLGNQPCF 300
           DSP+SIAKTVVD+LSQHHVVPKA SRELGENVPITVTDIHTRSSN CFDPNSDL NQPCF
Sbjct: 241 DSPQSIAKTVVDRLSQHHVVPKANSRELGENVPITVTDIHTRSSNGCFDPNSDLANQPCF 300

Query: 301 PTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQERFNQYRKFD 360
           PTDAPWKTVS HMYET KP ETNEDFDGELQKRAKEAEERVM+LSEELEQERFNQYRKFD
Sbjct: 301 PTDAPWKTVSEHMYETCKPSETNEDFDGELQKRAKEAEERVMYLSEELEQERFNQYRKFD 360

Query: 361 VSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE 420
           VSDLIQII+ILTGERFT ALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE
Sbjct: 361 VSDLIQIIKILTGERFTFALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE 420

Query: 421 LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETENRT 480
           LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKM TENRT
Sbjct: 421 LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMVTENRT 480

Query: 481 ITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD 540
           ITTNLEQNI+DLTAKIDEKNEENKYLQ+NLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD
Sbjct: 481 ITTNLEQNIVDLTAKIDEKNEENKYLQINLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD 540

Query: 541 LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVELAL 600
           LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQ ERLK+EQMRLTGVELAL
Sbjct: 541 LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQFERLKIEQMRLTGVELAL 600

Query: 601 RKELESYRVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE 660
           RKELES RVEVDSLR ENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE
Sbjct: 601 RKELESCRVEVDSLRRENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE 660

Query: 661 STQFCSKLLEFIKEKIDQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTMSLQ 720
           STQFCSKLLEFIKEK+ QF PTEHRMEHIKNGLDGQFF+ESE+KI+SLKHGIESLTMSLQ
Sbjct: 661 STQFCSKLLEFIKEKVGQFCPTEHRMEHIKNGLDGQFFIESEIKIQSLKHGIESLTMSLQ 720

Query: 721 KISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKELEVE 780
           KISMLLQAKSNPTSQTS VD  LQLNCQY EDGLRSELKAETLFSSLLREKLYSKELEVE
Sbjct: 721 KISMLLQAKSNPTSQTSGVDT-LQLNCQYPEDGLRSELKAETLFSSLLREKLYSKELEVE 780

Query: 781 QLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEESTR 840
           QLQ ELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKL  GVEESTR
Sbjct: 781 QLQAELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLHMGVEESTR 840

Query: 841 ELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETLEEDNLLKE 895
           ELESVK +LEK+SKERDMMLEEVNKYREKNMLLNSEVDVLKSN+ETLEED LLKE
Sbjct: 841 ELESVKAILEKVSKERDMMLEEVNKYREKNMLLNSEVDVLKSNVETLEEDILLKE 894

BLAST of Cucsat.G15096 vs. ExPASy TrEMBL
Match: A0A5A7SVN4 (Rho-associated protein kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00100 PE=4 SV=1)

HSP 1 Score: 1591 bits (4120), Expect = 0.0
Identity = 839/895 (93.74%), Postives = 863/895 (96.42%), Query Frame = 0

Query: 1   MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSSKAGSSPQSTRSGKHIDDSE 60
           MKKLFLRSFGTGHGKNNSA+PSTNES+THWE+P ESRTSSSKAGSSPQSTRSGKHIDDSE
Sbjct: 1   MKKLFLRSFGTGHGKNNSALPSTNESQTHWEHPFESRTSSSKAGSSPQSTRSGKHIDDSE 60

Query: 61  RFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSSRCQSPSRE 120
           RFG GPKLRRTRSLSSAAFRDQGQ+DFYGSSDPSR+PGNSSSGFK+Q EPSS CQSPSRE
Sbjct: 61  RFGMGPKLRRTRSLSSAAFRDQGQVDFYGSSDPSRTPGNSSSGFKQQCEPSSCCQSPSRE 120

Query: 121 MQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING 180
           MQF  KQMEMPNDYY SG +RPSSR CYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING
Sbjct: 121 MQFKTKQMEMPNDYYTSGFVRPSSRACYDSSGNSSTSVSSVSNRVLDRYIDGEQHQEING 180

Query: 181 SMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEEVGEYGFGN 240
           SM+KCSQR+NGWRPPRAQCLP+TSTTASIKDKPRSYSSREAKGSIS LLSEEVGEYGFGN
Sbjct: 181 SMNKCSQRNNGWRPPRAQCLPHTSTTASIKDKPRSYSSREAKGSISHLLSEEVGEYGFGN 240

Query: 241 DSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNSDLGNQPCF 300
           DSP+SIAKTVVD+LSQHHVVPKA SRELGENVPITVTDIHTRSSN CFDPNSDL NQPCF
Sbjct: 241 DSPQSIAKTVVDRLSQHHVVPKANSRELGENVPITVTDIHTRSSNGCFDPNSDLANQPCF 300

Query: 301 PTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQERFNQYRKFD 360
           PTDAPWKTVS HMYET KP ETNEDFDGELQKRAKEAEERVM+LSEELEQERFNQYRKFD
Sbjct: 301 PTDAPWKTVSEHMYETCKPSETNEDFDGELQKRAKEAEERVMYLSEELEQERFNQYRKFD 360

Query: 361 VSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE 420
           VSDLIQII+ILTGERFT ALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE
Sbjct: 361 VSDLIQIIKILTGERFTFALEISNLLQSRIADRTCAREELRQANAELESRTQKLEKEKIE 420

Query: 421 LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMETENRT 480
           LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKM TENRT
Sbjct: 421 LQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSLNKMVTENRT 480

Query: 481 ITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD 540
           ITTNLEQNI+DLTAKIDEKNEENKYLQ+NLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD
Sbjct: 481 ITTNLEQNIVDLTAKIDEKNEENKYLQINLSKLEEDYRGAIEGMDCIRKNYEEKEKECKD 540

Query: 541 LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQMRLTGVELAL 600
           LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQ ERLK+EQMRLTGVELAL
Sbjct: 541 LHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQFERLKIEQMRLTGVELAL 600

Query: 601 RKELESYRVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE 660
           RKELES RVEVDSLR ENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE
Sbjct: 601 RKELESCRVEVDSLRRENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQNQGLVLLNE 660

Query: 661 STQFCSKLLEFIKEKIDQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHGIESLTMSLQ 720
           STQFCSKLLEFIKEK+ QF PTEHRMEHIKNGLDGQFF+ESE+KI+SLKHGIESLTMSLQ
Sbjct: 661 STQFCSKLLEFIKEKVGQFCPTEHRMEHIKNGLDGQFFIESEIKIQSLKHGIESLTMSLQ 720

Query: 721 KISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREKLYSKELEVE 780
           KISMLLQAKSNPTSQTS VD  LQLNCQY EDGLRSELKAETLFSSLLREKLYSKELEVE
Sbjct: 721 KISMLLQAKSNPTSQTSGVDT-LQLNCQYPEDGLRSELKAETLFSSLLREKLYSKELEVE 780

Query: 781 QLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLQKGVEESTR 840
           QLQ ELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKL  GVEESTR
Sbjct: 781 QLQAELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKLHMGVEESTR 840

Query: 841 ELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETLEEDNLLKE 895
           ELESVK +LEK+SKERDMMLEEVNKYREKNMLLNSEVDVLKSN+ETLEED LLKE
Sbjct: 841 ELESVKAILEKVSKERDMMLEEVNKYREKNMLLNSEVDVLKSNVETLEEDILLKE 894

BLAST of Cucsat.G15096 vs. ExPASy TrEMBL
Match: A0A6J1CAU5 (myosin-13 OS=Momordica charantia OX=3673 GN=LOC111009977 PE=4 SV=1)

HSP 1 Score: 1285 bits (3325), Expect = 0.0
Identity = 697/920 (75.76%), Postives = 782/920 (85.00%), Query Frame = 0

Query: 1   MKKLFLRSFGTGHGKNNSAIPSTNESETHWENPLESRTSSS---KAGSSPQSTR-----S 60
           MKK F RS      K+N A PSTN+ + +WE+PLESR ++S   KAGSSPQST+     S
Sbjct: 1   MKKFFFRS------KHNIAPPSTNDCKAYWEHPLESRMNNSIGDKAGSSPQSTKHFSSKS 60

Query: 61  GKHIDDSERFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPSS 120
           G   +D ER  T PKLRRTRSLSSAAF DQGQ++FYG SDPSRSPG+     +RQHE SS
Sbjct: 61  GMQTNDIERSSTSPKLRRTRSLSSAAFGDQGQMNFYGPSDPSRSPGS-----QRQHEQSS 120

Query: 121 RCQSPSREMQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYIDG 180
           RCQ P+ EMQF  KQME+PNDYY  G +RP S+TCYDSSGNSSTS S+VSNRVLDRYIDG
Sbjct: 121 RCQGPTWEMQFKVKQMEVPNDYYT-GPVRPCSKTCYDSSGNSSTSSSNVSNRVLDRYIDG 180

Query: 181 EQHQEINGSMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSEE 240
           EQHQEI+GS +K SQ++NGWRPPRAQCL  +S TASIKDKPRSYSSRE K S SR  S E
Sbjct: 181 EQHQEISGSKNKYSQKNNGWRPPRAQCLTPSSPTASIKDKPRSYSSREVKSSHSRFSSRE 240

Query: 241 VGEYGFGNDSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPNS 300
           +GEYGFGN+SPRSIAK VVD+LSQ+HV+PKATS+ELGENVPI  TDI  +S N C+DPN 
Sbjct: 241 LGEYGFGNESPRSIAKNVVDRLSQYHVLPKATSKELGENVPIRTTDIQNQSLNGCYDPNL 300

Query: 301 DLGNQPCFPTDAPWKTVSG-----------------HMYETYKPGETNEDFDGELQKRAK 360
           D+  +PCFPTD P +TVSG                  MYE  K GET+ D DGELQ+  K
Sbjct: 301 DVVTRPCFPTDEPCETVSGPHTEELSGFHKQKISPGRMYEGCKSGETDGDLDGELQRSVK 360

Query: 361 EAEERVMFLSEELEQERFNQYRKFDVSDLIQIIRILTGERFTLALEISNLLQSRIADRTC 420
           EA+ER+MFLSEELEQERF QYRKFDVSDLIQ+I+ L+ +RFTLALEIS+LLQSRIADR  
Sbjct: 361 EADERIMFLSEELEQERFVQYRKFDVSDLIQVIKNLSEDRFTLALEISSLLQSRIADRES 420

Query: 421 AREELRQANAELESRTQKLEKEKIELQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVR 480
           A+EELRQANAEL+SRT+KLEKEK ELQ+GLEKELDRRS+DWSFKLEKY+LEE+GLRGRVR
Sbjct: 421 AKEELRQANAELDSRTRKLEKEKTELQLGLEKELDRRSSDWSFKLEKYKLEEQGLRGRVR 480

Query: 481 ELAEQNVSLQREVSSLNKMETENRTITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEE 540
           ELAEQNVSLQREVSSLNK ETEN++  TNLEQN++DLT +IDEKNE+N YLQLNLSKLEE
Sbjct: 481 ELAEQNVSLQREVSSLNKTETENKSTITNLEQNVLDLTTRIDEKNEQNNYLQLNLSKLEE 540

Query: 541 DYRGAIEGMDCIRKNYEEKEKECKDLHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQP 600
           DYRGA EGMDCIRKN+EEKEKEC++LHKSITRLSRTC+EQEKTIDGLRERLSEQF NIQP
Sbjct: 541 DYRGATEGMDCIRKNFEEKEKECRELHKSITRLSRTCSEQEKTIDGLRERLSEQFVNIQP 600

Query: 601 VEKFDKQCERLKMEQMRLTGVELALRKELESYRVEVDSLRHENIKILTRLKDNGNESGAI 660
           VEKFDK  E+LKMEQMRLTGVE+ALRKELESYRVEVDSLRHENI ILTRLKDNGNESGAI
Sbjct: 601 VEKFDKHFEKLKMEQMRLTGVEMALRKELESYRVEVDSLRHENINILTRLKDNGNESGAI 660

Query: 661 TFKLDNEMSARVYHLQNQGLVLLNESTQFCSKLLEFIKEKIDQFHPTEHRMEHIKNGLDG 720
            FKLDNEMS+RVYHLQNQGLVLL ESTQFCSKLLEFIKEK+ Q   T+HRMEH+KNGLDG
Sbjct: 661 NFKLDNEMSSRVYHLQNQGLVLLKESTQFCSKLLEFIKEKVGQLQQTKHRMEHMKNGLDG 720

Query: 721 QFFLESEMKIRSLKHGIESLTMSLQKISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLR 780
           QFF+ESE KI+  KHGIESLTMSL + SM+LQAKSNPTSQ+S VDNALQ+N QY ED LR
Sbjct: 721 QFFVESETKIQGFKHGIESLTMSLHRTSMVLQAKSNPTSQSSGVDNALQINSQYPEDVLR 780

Query: 781 SELKAETLFSSLLREKLYSKELEVEQLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMK 840
           SELKAETL +SLLREKLYSKELEVEQLQ ELVTAVRGNDILKCEVQN MD LSCLTHKMK
Sbjct: 781 SELKAETLLTSLLREKLYSKELEVEQLQAELVTAVRGNDILKCEVQNQMDSLSCLTHKMK 840

Query: 841 DLELQLRMKNEEISKLQKGVEESTRELESVKEVLEKISKERDMMLEEVNKYREKNMLLNS 895
           DLELQL  KN++I KLQ G EESTRELE+++++LEKISKERDM+ EE NKYRE NMLLNS
Sbjct: 841 DLELQLLKKNDDIFKLQNGFEESTRELETLRDILEKISKERDMVWEEGNKYRENNMLLNS 900

BLAST of Cucsat.G15096 vs. ExPASy TrEMBL
Match: A0A6J1FM18 (golgin subfamily B member 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447046 PE=4 SV=1)

HSP 1 Score: 1272 bits (3292), Expect = 0.0
Identity = 698/904 (77.21%), Postives = 779/904 (86.17%), Query Frame = 0

Query: 1   MKKLFLRSFGTGHGKNNSAIPS-TNESETHWENPLESRTSS---SKAGSSPQSTRS---- 60
           MKK FLRSFG G+GKN+S  PS T++SE +WE+PL SR  +    KAGSSPQ ++     
Sbjct: 1   MKKFFLRSFGNGYGKNDSVHPSSTDDSEAYWEHPLGSRMGTPIGDKAGSSPQRSKDLPSK 60

Query: 61  -GKHIDDSERFGTGPKLRRTRSLSSAAFRDQGQIDFYGSSDPSRSPGNSSSGFKRQHEPS 120
             + IDD+ER  + PKLRRT+SLSSAAFRDQGQI+F G  DPSRSPGN+SS  KRQHE S
Sbjct: 61  FNRQIDDNERSRSRPKLRRTQSLSSAAFRDQGQINFDGLIDPSRSPGNASSRSKRQHEQS 120

Query: 121 SRCQSPSREMQFNAKQMEMPNDYYASGSIRPSSRTCYDSSGNSSTSVSSVSNRVLDRYID 180
           SRCQSPSREMQF  KQ E+PNDYY SGS RP SRT YDSSGNS+T+ S VSNRVLDRYID
Sbjct: 121 SRCQSPSREMQFKVKQTELPNDYYTSGSFRPCSRTWYDSSGNSTTTSSIVSNRVLDRYID 180

Query: 181 GEQHQEINGSMSKCSQRSNGWRPPRAQCLPYTSTTASIKDKPRSYSSREAKGSISRLLSE 240
           GEQHQEINGS +K SQR+NGWRPPRAQCLP +STTASIKD PRSYSSRE + S+SR LSE
Sbjct: 181 GEQHQEINGSKNKYSQRNNGWRPPRAQCLPPSSTTASIKDNPRSYSSRETRSSLSRFLSE 240

Query: 241 EVGEYGFGNDSPRSIAKTVVDKLSQHHVVPKATSRELGENVPITVTDIHTRSSNECFDPN 300
           + GEYGFGNDSPRS AKTVVD+LSQ HVVP+ + +ELGEN+PITV D ++RS N CFDPN
Sbjct: 241 D-GEYGFGNDSPRS-AKTVVDRLSQQHVVPRGSYKELGENIPITVADTYSRSLNGCFDPN 300

Query: 301 SDLGNQPCFPTDAPWKTVSGHMYETYKPGETNEDFDGELQKRAKEAEERVMFLSEELEQE 360
           +DL  +PC PTD              +PGET    DGELQK+AKEAEER+MFLSEELEQE
Sbjct: 301 ADL-TKPCIPTD--------------EPGET----DGELQKKAKEAEERIMFLSEELEQE 360

Query: 361 RFNQYRKFDVSDLIQIIRILTGERFTLALEISNLLQSRIADRTCAREELRQANAELESRT 420
           R  QY KFDVSDLIQII+ LTGERFTLALE+S+LLQSRIADRTCAREELRQAN ELESRT
Sbjct: 361 RLVQYSKFDVSDLIQIIKNLTGERFTLALEVSSLLQSRIADRTCAREELRQANEELESRT 420

Query: 421 QKLEKEKIELQVGLEKELDRRSNDWSFKLEKYQLEEEGLRGRVRELAEQNVSLQREVSSL 480
           QKLEKEK ELQVGLEKELDRRS+DWSFKLEKY+LEEEG RGRVRELAEQNVSLQREV+SL
Sbjct: 421 QKLEKEKTELQVGLEKELDRRSSDWSFKLEKYKLEEEGQRGRVRELAEQNVSLQREVASL 480

Query: 481 NKMETENRTITTNLEQNIMDLTAKIDEKNEENKYLQLNLSKLEEDYRGAIEGMDCIRKNY 540
           NK ETEN+++TTNLEQNI+DLTA+IDEKNE+NKYLQLNLSKLEEDYRG+IEGMDCIRKN+
Sbjct: 481 NKRETENKSMTTNLEQNILDLTARIDEKNEQNKYLQLNLSKLEEDYRGSIEGMDCIRKNF 540

Query: 541 EEKEKECKDLHKSITRLSRTCNEQEKTIDGLRERLSEQFSNIQPVEKFDKQCERLKMEQM 600
           EEKEKEC++LHKSITRL+RTCNEQEKTI+GLRERLSEQF N QP+EK DK+ E+LKMEQM
Sbjct: 541 EEKEKECRELHKSITRLTRTCNEQEKTINGLRERLSEQFGNSQPMEKLDKEFEKLKMEQM 600

Query: 601 RLTGVELALRKELESYRVEVDSLRHENIKILTRLKDNGNESGAITFKLDNEMSARVYHLQ 660
           RLTGVELALRK LES RVEVDSLR ENI ILT LKDNGNE GA TFKL NEMS RVYHLQ
Sbjct: 601 RLTGVELALRKALESCRVEVDSLRRENINILTHLKDNGNERGATTFKLVNEMSTRVYHLQ 660

Query: 661 NQGLVLLNESTQFCSKLLEFIKEKIDQFHPTEHRMEHIKNGLDGQFFLESEMKIRSLKHG 720
           NQG+VLLNESTQFCS+LLEFIKEK  Q HP +HR EHI+NGLD  FFLESE KI+  K+G
Sbjct: 661 NQGMVLLNESTQFCSQLLEFIKEKAAQLHPNKHRTEHIENGLDAHFFLESEAKIQGFKYG 720

Query: 721 IESLTMSLQKISMLLQAKSNPTSQTSDVDNALQLNCQYLEDGLRSELKAETLFSSLLREK 780
           IESLTMSLQKISMLLQA+SN TSQ+S VDNALQLN QY EDGLRSELKAETLFSSLLREK
Sbjct: 721 IESLTMSLQKISMLLQAESNSTSQSSGVDNALQLNSQYSEDGLRSELKAETLFSSLLREK 780

Query: 781 LYSKELEVEQLQTELVTAVRGNDILKCEVQNGMDGLSCLTHKMKDLELQLRMKNEEISKL 840
           L+SKELEVEQLQ EL TAVRGND+LKCEVQNGM+GLSCL+HK+KDLELQL  +NE+I+KL
Sbjct: 781 LFSKELEVEQLQAELATAVRGNDMLKCEVQNGMEGLSCLSHKIKDLELQLLKRNEDINKL 840

Query: 841 QKGVEESTRELESVKEVLEKISKERDMMLEEVNKYREKNMLLNSEVDVLKSNIETLEEDN 895
           Q  +EES RELE +++VL+KISKERDM+ EEVNK+REKNMLL S+VD LKS IETLEED 
Sbjct: 841 QTELEESRRELEILRDVLQKISKERDMLWEEVNKHREKNMLLISKVDELKSKIETLEEDI 883