Cucsat.G14700 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G14700
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	EH domain-containing protein 1
Location	ctg1869: 3667809 .. 3670225 (+)
RNA-Seq Expression	Cucsat.G14700
Synteny	Cucsat.G14700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTGTTCATCCCGAGCGCCAAACTGAAGAAAAAGGATAAAGAAATCAGAGAAGAGTTGATAGTAGAAAGAATCGGGTACATCGTTCTTCAAGTTTTGAATTTCATTTCTCTCCTTCTTGTTTCAATGTGGAAATTGGATCGGGTGCGATTGGCCGATTCAGGTACTTTTCTATTATCAATCCGCGTTTCTTCTTCTATTTTGTTCATCGATGATGTTTATCGTTTTGTTTTCTGTTCCATTCTTTCCGTGTAGATGGAGATGGTCGTGTTACTGGAAATGATGCTATACAGTTCTTCTCCATGTCTCATCTTTCTCGCGTCGAGCTCAAACAGGTATCTCATTTGTTTTTCTTTTAGATTATTGAGTTAATAGTTCGATCTAGCTCTGACGCCTCGATGGATAGAGTATTGTGATTTGTCTTAGTTGCGAATATTTTAATTGATGTGAAGTTTAATTTAACTTCGATGTGTTTCGTGGTGAATATCAGTATTCGAAACTTCCTTTATCTGAATGAGAATTCAAGAAACTTGTTTATTTCTTTATTTAGTCAATTATCGTATTTACTCTGATTTGTTTTGTGATCAGAGATTTTCGTTTGGTTTTGTTCTTAAAAAAATTGTGCTTATTGTTTCTCTATCTTTTTTTCGTACTCACGATTGAGAAGTTTTTTAATGGCAAACTTAGGAGTATTTTAACTATATATAGATACACTGGATTTATTGAATTTAGTATATGCCTGTGCTGTCTTTTCCTTTCTTTGTATGACACGGGGTTTACTTATTTGAGTGTATACTATGTATTGGATTCTGTGGTTACATCATTTGCCCTTTTGCCTTTTCTACGATGCCAAGTGAAACTAACGTGATATTTCATATCATGTTTAATGGTTTGGGCTGGTGCAGACTCCAAACGACAGGGATATTTAGGGTTCAACGAGTTCGTTACGGCCATGCAGGTTTTATACTACTTATTTGGATTTTATTACATTAACCAATACGCAGATGTTTTTCTAATTCTAAATATTGTTTTCATTACAACCAGCTAATTTCTTTAGCACAAGCAGGGTATGACCTGGACTCAGATATCCTCAAGAACGCAGGCAAGACATCTTAACAATTCAAAGTTTCTTTGGCTTTTATTCTTGGAGTGTAACTGATTAATTTCTGTTCTGGATCAAGACATCAAATAATTCAGACATCTTATGCCAGAGCCTTTAATTTTATGCCATGTCTGAATTTATCAGTTGTATATTAGATCTATTGATTTATTTTTACTTTATCTTCCTCTAATCCTCGAAATGTGACAGTGCTCTCGTTGTATATAATTCAAGAAGAAACGTAGTCTAAGCAATTTTTTTGTAGACTGCCCAGGTGAGAATATTGAGATTCAGGGACCGTAATTCTTTCTAACCGGTTAATTCATCCTCTAATGGTTGGTTCCTTGATTGACTTTATTGTATGTTATTGAACTAGATTATTTGAAAACTATTAGTGAAAAGTTATAACAGGCAGAATGGATAAAATCTGGTTTGTAGATTGTAAACAGAAAGCGGTTTGTTTGAATTCAAACTTTGTATTCATGTGCTTACATAACACCTTGCAATGCAAAGGCACTACTTGCTTATTGTTCATTATACATTGACACCCTTCTGCTTCCTTACAGCTGGCATGGAGGAAATTAAACTTCCAGTGCCAGAAGGGTTGGACGCTTTAGCTGTTGTAAGTACTCACTCCTTGACAGTGTTGAATTTCCATTGAAATATAGATAAATTAATGTAAGATATCATTTTGCTGATACGCTTTTCAGAAAACTAAGAGATTAGCAATATCCAGTCAACCTGAGACAAATGGTTAGAAAATTACTGATCATTGAATTTCCATAATAAAATTGCCAACTCGGTCTCTGATTTAAATTGATACTCTGCCAATATAGGAACTTTTCAACCTATGCCCCCACCATCGACTCCATGGTTTGCTTCAAAATCTGGGAGCAAGGTATTATGGGTTCTTTTTTCCATTTTTGAGCATTAACTAAAAAAAGTTCTCAAAATTTTGAATTCTAACAAAACTTTGCATTAGCAATGCACCTACAGAGTTGAGATGCTATATAATATTATTATTATATGTATGTATATATTTTACTCATAAGGATTTTTGAAGTTCTTCGGAGACTTTGGAATGTCATAGCCAAACAAGAACTTCAAAAATTCTTATGAGTAAAATATATATATAAATACATATAATAGCAATATTACATAGCATTTCAATCTTTGTAGTTGGATTGTTACTTGCAATGAAGCAAAGTTTCGTTAGAATTCAAAATTTTGGGAACTTTTTTTCTTTATCCATTTTCTTTTAAGTTAATGCTCAAATGTATGTGTATCTCTTGATTGGGCATTTTGGTGTATTCTTCAA

Coding sequence (CDS)

ATGGAGATGGTCGTGTTACTGGAAATGATGCTATACAGTTCTTCTCCATGTCTCATCTTTCTCGCGTCGAGCTCAAACAGACTCCAAACGACAGGGATATTTAGGGTTCAACGAGTTCGTTACGGCCATGCAGGGTATGACCTGGACTCAGATATCCTCAAGAACGCAGCTGGCATGGAGGAAATTAAACTTCCAGTGCCAGAAGGGTTGGACGCTTTAGCTGTTAAAACTAAGAGATTAGCAATATCCAGTCAACCTGAGACAAATGGAACTTTTCAACCTATGCCCCCACCATCGACTCCATGGTTTGCTTCAAAATCTGGGAGCAAGGTATTATGGGTTCTTTTTTCCATTTTTGAGCATTAA

Protein sequence

MEMVVLLEMMLYSSSPCLIFLASSSNRLQTTGIFRVQRVRYGHAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPMPPPSTPWFASKSGSKVLWVLFSIFEH

Homology

[Close]

BLAST of Cucsat.G14700 vs. ExPASy Swiss-Prot
Match: B3LF48 (EH domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=EHD2 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 2.1e-05
Identity = 26/75 (34.67%), Postives = 40/75 (53.33%), Query Frame = 0


Query: 33  IFRVQRVRYGHAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTF 92
           I  ++ V     G+++ SD+LK +  M+ ++LPV EGL+ +  K K    +   E N   
Sbjct: 75  ITAMKLVSLAQEGHEITSDLLKGSIDMKSVELPVLEGLENVVSKQKVSKTNVDVEDNVVT 134

Query: 93  QPMPPPSTPWFASKS 108
           +P     TPWF SKS
Sbjct: 135 KPQVTAKTPWFKSKS 149

[Close]

BLAST of Cucsat.G14700 vs. NCBI nr
Match: XP_031737930.1 (EH domain-containing protein 2-like isoform X1 [Cucumis sativus])

HSP 1 Score: 237 bits (605), Expect = 1.07e-78
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0


Query: 1   MEMVVLLEMMLYSSSPCLIFLASSSNRLQTTGIFRVQRVRYGHAGYDLDSDILKNAAGME 60
           MEMVVLLEMMLYSSSPCLIFLASSSNRLQTTGIFRVQRVRYGHAGYDLDSDILKNAAGME
Sbjct: 1   MEMVVLLEMMLYSSSPCLIFLASSSNRLQTTGIFRVQRVRYGHAGYDLDSDILKNAAGME 60

Query: 61  EIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPMPPPSTPWFASKSGSKVLWVLFSIFE 120
           EIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPMPPPSTPWFASKSGSKVLWVLFSIFE
Sbjct: 61  EIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPMPPPSTPWFASKSGSKVLWVLFSIFE 120

[Close]

BLAST of Cucsat.G14700 vs. NCBI nr
Match: XP_031737934.1 (EH domain-containing protein 2-like isoform X3 [Cucumis sativus])

HSP 1 Score: 160 bits (406), Expect = 7.92e-49
Identity = 79/86 (91.86%), Postives = 81/86 (94.19%), Query Frame = 0


Query: 36  VQRVRYGHAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM 95
           +Q +    AGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM
Sbjct: 1   MQLISLAQAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM 60

Query: 96  PPPSTPWFASKSGSKVLWVLFSIFEH 121
           PPPSTPWFASKSGSKVLWVLFSIFEH
Sbjct: 61  PPPSTPWFASKSGSKVLWVLFSIFEH 86

[Close]

BLAST of Cucsat.G14700 vs. NCBI nr
Match: XP_031737932.1 (uncharacterized protein LOC105434818 isoform X2 [Cucumis sativus])

HSP 1 Score: 144 bits (364), Expect = 3.88e-42
Identity = 75/76 (98.68%), Postives = 76/76 (100.00%), Query Frame = 0


Query: 1  MEMVVLLEMMLYSSSPCLIFLASSSNRLQTTGIFRVQRVRYGHAGYDLDSDILKNAAGME 60
          MEMVVLLEMMLYSSSPCLIFLASSSNRLQTTGIFRVQRVRYGHAGYDLDSDILKNAAGME
Sbjct: 1  MEMVVLLEMMLYSSSPCLIFLASSSNRLQTTGIFRVQRVRYGHAGYDLDSDILKNAAGME 60

Query: 61 EIKLPVPEGLDALAVK 76
          EIKLPVPEGLDALAV+
Sbjct: 61 EIKLPVPEGLDALAVE 76

[Close]

BLAST of Cucsat.G14700 vs. NCBI nr
Match: XP_004145425.2 (EH domain-containing protein 1 [Cucumis sativus] >KAE8653637.1 hypothetical protein Csa_007087 [Cucumis sativus])

HSP 1 Score: 128 bits (321), Expect = 8.32e-32
Identity = 64/76 (84.21%), Postives = 68/76 (89.47%), Query Frame = 0


Query: 36  VQRVRYGHAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM 95
           +Q +    AGYDLDSDILK AAGMEEIKLPV EGLDALAVKTKRLAISSQ ETNGTFQPM
Sbjct: 77  MQLISLAQAGYDLDSDILKKAAGMEEIKLPVLEGLDALAVKTKRLAISSQHETNGTFQPM 136

Query: 96  PPPSTPWFASKSGSKV 111
           PPPSTPWFA+KSGSK+
Sbjct: 137 PPPSTPWFATKSGSKI 152

[Close]

BLAST of Cucsat.G14700 vs. NCBI nr
Match: TYK12336.1 (EH domain-containing protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 127 bits (319), Expect = 1.14e-31
Identity = 63/76 (82.89%), Postives = 68/76 (89.47%), Query Frame = 0


Query: 36  VQRVRYGHAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM 95
           +Q +    AGYDLDSDILK AAGMEEIKLPV EGLDALAVK+KRLA+SSQ ETNGTFQPM
Sbjct: 77  MQLISLAQAGYDLDSDILKKAAGMEEIKLPVLEGLDALAVKSKRLAVSSQHETNGTFQPM 136

Query: 96  PPPSTPWFASKSGSKV 111
           PPPSTPWFASKSGSK+
Sbjct: 137 PPPSTPWFASKSGSKI 152

[Close]

BLAST of Cucsat.G14700 vs. ExPASy TrEMBL
Match: A0A0A0LQH1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042770 PE=4 SV=1)

HSP 1 Score: 159 bits (403), Expect = 1.10e-48
Identity = 78/86 (90.70%), Postives = 81/86 (94.19%), Query Frame = 0


Query: 36  VQRVRYGHAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM 95
           +Q +    AGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM
Sbjct: 1   MQLISLAQAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM 60

Query: 96  PPPSTPWFASKSGSKVLWVLFSIFEH 121
           PPPSTPWFA+KSGSKVLWVLFSIFEH
Sbjct: 61  PPPSTPWFATKSGSKVLWVLFSIFEH 86

[Close]

BLAST of Cucsat.G14700 vs. ExPASy TrEMBL
Match: A0A5D3CPI7 (EH domain-containing protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G001110 PE=4 SV=1)

HSP 1 Score: 127 bits (319), Expect = 5.52e-32
Identity = 63/76 (82.89%), Postives = 68/76 (89.47%), Query Frame = 0


Query: 36  VQRVRYGHAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM 95
           +Q +    AGYDLDSDILK AAGMEEIKLPV EGLDALAVK+KRLA+SSQ ETNGTFQPM
Sbjct: 77  MQLISLAQAGYDLDSDILKKAAGMEEIKLPVLEGLDALAVKSKRLAVSSQHETNGTFQPM 136

Query: 96  PPPSTPWFASKSGSKV 111
           PPPSTPWFASKSGSK+
Sbjct: 137 PPPSTPWFASKSGSKI 152

[Close]

BLAST of Cucsat.G14700 vs. ExPASy TrEMBL
Match: A0A1S3C8M9 (EH domain-containing protein 1 OS=Cucumis melo OX=3656 GN=LOC103498230 PE=4 SV=1)

HSP 1 Score: 127 bits (319), Expect = 7.71e-32
Identity = 63/76 (82.89%), Postives = 68/76 (89.47%), Query Frame = 0


Query: 36  VQRVRYGHAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM 95
           +Q +    AGYDLDSDILK AAGMEEIKLPV EGLDALAVK+KRLA+SSQ ETNGTFQPM
Sbjct: 77  MQLISLAQAGYDLDSDILKKAAGMEEIKLPVLEGLDALAVKSKRLAVSSQHETNGTFQPM 136

Query: 96  PPPSTPWFASKSGSKV 111
           PPPSTPWFASKSGSK+
Sbjct: 137 PPPSTPWFASKSGSKI 152

[Close]

BLAST of Cucsat.G14700 vs. ExPASy TrEMBL
Match: A0A6J1EIE2 (EH domain-containing protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434367 PE=4 SV=1)

HSP 1 Score: 120 bits (301), Expect = 2.59e-29
Identity = 60/76 (78.95%), Postives = 65/76 (85.53%), Query Frame = 0


Query: 36  VQRVRYGHAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM 95
           +Q +    AGYDLDSDILK AAGMEEIKLPV +GLDAL  KTKRLAISS+ ETNGT QPM
Sbjct: 77  MQLISLAQAGYDLDSDILKKAAGMEEIKLPVLDGLDALVAKTKRLAISSKHETNGTTQPM 136

Query: 96  PPPSTPWFASKSGSKV 111
           PPPSTPWFASKSGSK+
Sbjct: 137 PPPSTPWFASKSGSKI 152

[Close]

BLAST of Cucsat.G14700 vs. ExPASy TrEMBL
Match: A0A6J1KGW5 (EH domain-containing protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495658 PE=4 SV=1)

HSP 1 Score: 115 bits (288), Expect = 1.68e-27
Identity = 58/76 (76.32%), Postives = 64/76 (84.21%), Query Frame = 0


Query: 36  VQRVRYGHAGYDLDSDILKNAAGMEEIKLPVPEGLDALAVKTKRLAISSQPETNGTFQPM 95
           +Q +    AGYDL+SDILK AAGMEEIKLPV +GLDAL  KTKRLAISS+ ETNGT Q M
Sbjct: 77  MQLISLAQAGYDLNSDILKKAAGMEEIKLPVLDGLDALVAKTKRLAISSKHETNGTTQSM 136

Query: 96  PPPSTPWFASKSGSKV 111
           PPPSTPWFASKSGSK+
Sbjct: 137 PPPSTPWFASKSGSKI 152

The following BLAST results are available for this feature:

BLAST of Cucsat.G14700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B3LF48	2.1e-05	34.67	EH domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=EHD2 PE=1 SV=1	[more]

BLAST of Cucsat.G14700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031737930.1	1.07e-78	100.00	EH domain-containing protein 2-like isoform X1 [Cucumis sativus]	[more]
XP_031737934.1	7.92e-49	91.86	EH domain-containing protein 2-like isoform X3 [Cucumis sativus]	[more]
XP_031737932.1	3.88e-42	98.68	uncharacterized protein LOC105434818 isoform X2 [Cucumis sativus]	[more]
XP_004145425.2	8.32e-32	84.21	EH domain-containing protein 1 [Cucumis sativus] >KAE8653637.1 hypothetical prot...	[more]
TYK12336.1	1.14e-31	82.89	EH domain-containing protein 1 [Cucumis melo var. makuwa]	[more]

BLAST of Cucsat.G14700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQH1	1.10e-48	90.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042770 PE=4 SV=1	[more]
A0A5D3CPI7	5.52e-32	82.89	EH domain-containing protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3C8M9	7.71e-32	82.89	EH domain-containing protein 1 OS=Cucumis melo OX=3656 GN=LOC103498230 PE=4 SV=1	[more]
A0A6J1EIE2	2.59e-29	78.95	EH domain-containing protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC11143436...	[more]
A0A6J1KGW5	1.68e-27	76.32	EH domain-containing protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495658 ...	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G14700.T4	Cucsat.G14700.T4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006897	endocytosis
biological_process	GO:0051260	protein homooligomerization
biological_process	GO:0032956	regulation of actin cytoskeleton organization
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0005525	GTP binding