Cucsat.G14657 (gene) Cucumber (B10) v3

Overview
NameCucsat.G14657
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionExocyst subunit Exo70 family protein
Locationctg1869: 3008642 .. 3011741 (+)
RNA-Seq ExpressionCucsat.G14657
SyntenyCucsat.G14657
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GATACTCGTATATTCAAAATGGACAGTGTCGTGTGAATACAATGCAGAGCGGTCAACAAGTCAAAGCGACGAGACATAAAGAAAGGAAAAAAGAGACGATCATTTCATATTTCATGGAAAGAAACTACAAAGAAATCTTGTTACGAAATTTCAAACTCAATTTCTTTCAATGTTTCTTCATTATTGCCTTTCACTTTTCATCACAATTCTTCAAGTAACAATGCTTCCGACGTTCCTTTTCTCATGCTGATCGGCTGTTTTCCGTCGCTTCTTCTCAACTTTCCTCTCATTTTTTTTCTGAAGAACGGCTGGATTAACGGCCAATTACTTTAGATCTTGCTTTCTGCACTACAAAATCAGGTCATTCTTTATTTTTTAACATTTTAGCAAGTTCTTCGTTTCGGTTTCTTCTGTTTTTTTTATTGTATTCGGTTTTTTTTTTTTTTAGCTTGAGGAATTTTAAGTGTTTTTGGTCCTTAGTCGTTCACTGTATTGTTTAGATTTCAATTCTAGAGGAAGCATGCCGTAGACTGTACGGTAGTGTTCTGAACTTTGATTCTTTGTACTTTTTTAGAACATCTCTGTTAAGCTAAACAAGCCAAAATACGATGAAATTTGTTCTGTTTGTACTTTGTAGTATGATTTATTATAATTGGGATGGGAACGAGGTGAAGTGAATTATTAGATGACATAATGGGCTATGAGATGAGTAAGCTATATATCAAAACTAATTGGCTTTAAGATGATAAGCTATATCTCTCTCTTTTTTTTTTTTTGTTCATAAGGTATCTTTATTTATTTTAATGAGTTAAATCCAACCTTCTTCTTGAAGTCATTGTTCTTTTTATTAGATTACAATTTTTGGTTACATGAGGGCTTGAGTCTCTATCTCAAAAACAATTTGCACGTGAGAAGAGTAACTTTTCTAATGTAGATGCTCAACGTGGGGAAGAGTTGGGGACAATTAGAAATGAATAGGAAATCAAGAGAGTAAACTTTTGGTTGGAAAGTGCTTTGTGATTTCACATGGCTGTTGATTTATGTCTTTCTTCCTAGTCAGATGGGGGATTGTGAATCAATTATACCAACCTCTGAAGGGGAGCAACACGCTCTAGCTGCATTTCAGCACCTAGTCAAGTTCTTAAGGACAAGTCAGAACGTGAATAATGAATTGAAAAAGCTCCTTGCCGATCTCGATTCTCATTTGACTTCCTTGACGTCGTATACTGTTGGAAAGTTGAGTGAGTTAGAGACAAGGTTCAAATCTGCCAAGGAGAAGATTACGAGATGGGAATCTAACAAATCCATGATATGGGATTCTGGTCCCAAAGAGGCTTCTGAATATATGAAGGCTGTTGATGAAATTCATAAAGTTCAGGAAGGTCTTAGAAGTTTATCTGCAAATGACAGTCAAAAGCAAAATGAACTTCTTTTCCAGGCCGACTCTGTCGTACAGATTGCAATGGCAAGGCTAGAGCAGGAGCTAATCCACATTCTTGCTCAGCACAAACTATATTTTGAGCCTGACTATGTTCCCTTCCAGTCTGGTGGCTATGATGTTGTCTATAACGAGTCCTTTGTTTCAGCCGAGGACAGTTTGGAGGTGGAAACTTCCCACGGAGAGAGTATTCCTGCTTTATATGCTGTGAACTTGGTCCATCCACACGTGATCGCTCATCTTAAATCCATCGCAAATGTTATGTTTAATTCAAATTATATTCAGGAATTTTGCCTGGCTTTTGTTAGAATGCGGAAAGATGCGTTGAACGAGTATCTGTTCATTCTCGAAATGGAGAAGTTTAGCATTGGAGATTTGCTGACCATGGAATGGAGCGACTTAAACGTGAAGATCAAGAAATGGGTTTGGCTTATTAAAATTGTTATTAGAGTCTACCTTACTAGTGAGCAAAGACTCTGTAACAATATATTTGAAGGCATTGGATCTTATAGTGCAGTCTGCTTTACTGAGATTTCTGCGACTTCTATGCTTCGCCTGTTGAATTTTGGAGAGGCCATAGCTATGGAACCCCATCGACCGGAAAAGCTGTTTCACTTGCTAGACATGTATGAGGTCCTAAAAAATCTTCTTGAATTTGTAGATGAATTGTTTTCCGAAGAAACAGAAAAGGGTTCTTTTCTCAAATTCGAGTTCCATAATCTTTTCAAGAAACTGGGTGTTTCTGCAAGGGCTACGTTTTTAGATTTTGGAAATTTCATTGCATGTTCCATATCAACAAACCCTTTTCCCGGCGGTGGTGTTCATCATCTAACAAGGTATGTAATGAACTACATCCATACTCTTACTGTTTTCAGAGACTCGCTTGTTTTCCTTCTCCAAGATCAAGCAACAGATGTCTTAAGCCCAACTACTGAGCTGCAGAGTGAAGTCAATAGCATACCTTGCCCAATGGCTTACCACCTTCAGTCAATCACTTCACATTTGCTATCCAATCTCAATAACAAATCCAAGTTGTACAAGGATGATGCTCTACGACATATTTTCTTAATGAATAACATCCATTATATAGTTCAAAAGGTTGAAAATTCTGATCTCATAGCTTTTCTTGGAAGTGGGTGGATGCGAGAACATATAAGAATGTTTCAAAGCCACGCAACTATCTATATGAGAGCCACCTGGCAGTCTGTTCTTTCTCTGCTAAGGCTTGATGGTGATGGAATGAAAACTTCAAAGGCAGTCTTTAAAGAAAAGTACAGGGCCTTCAATGCAGCCTTTGAGGAGATATATAAGAACCAAACTGGTTGGAATGTCCCAGATCCTCAACTACGTGATGACTTGCTTATTCAAACCTCAAATTGTGTAATTCAAGCATATCGAATCCTCTGCGGGTCAAGATCTCAATTTAACCGTGAAAAGTATATCAAATATACAACTGATGATCTGAGCAAGCATATGCTGGATCTCTTGCAAGGCTCATCGCGCTCGTTGCAAAGTTCACGCCGGAGATGATAACTGTTGCTGGTAAAGGTTTTAGAATTCATCAGCCCCTTTCGACATTCATGTCTTCCTTTTCTCGTTTTCCTTATAGGCACTTGTTAATTATATTGTTCAACAGCCAATCTTGCATAAGACTCTAGCATGCATCCCTATACCTACACAATCTATTCATTTATACTGAATATCAATTCAACTTGTAATACTCACAACTCCTCCTTGTATAAAGTTGACAGATATTGATTACTATCAATAGTCCTATC

Coding sequence (CDS)

ATGGGGGATTGTGAATCAATTATACCAACCTCTGAAGGGGAGCAACACGCTCTAGCTGCATTTCAGCACCTAGTCAAGTTCTTAAGGACAAGTCAGAACGTGAATAATGAATTGAAAAAGCTCCTTGCCGATCTCGATTCTCATTTGACTTCCTTGACGTCGTATACTGTTGGAAAGTTGAGTGAGTTAGAGACAAGGTTCAAATCTGCCAAGGAGAAGATTACGAGATGGGAATCTAACAAATCCATGATATGGGATTCTGGTCCCAAAGAGGCTTCTGAATATATGAAGGCTGTTGATGAAATTCATAAAGTTCAGGAAGGTCTTAGAAGTTTATCTGCAAATGACAGTCAAAAGCAAAATGAACTTCTTTTCCAGGCCGACTCTGTCGTACAGATTGCAATGGCAAGGCTAGAGCAGGAGCTAATCCACATTCTTGCTCAGCACAAACTATATTTTGAGCCTGACTATGTTCCCTTCCAGTCTGGTGGCTATGATGTTGTCTATAACGAGTCCTTTGTTTCAGCCGAGGACAGTTTGGAGGTGGAAACTTCCCACGGAGAGAGTATTCCTGCTTTATATGCTGTGAACTTGGTCCATCCACACGTGATCGCTCATCTTAAATCCATCGCAAATGTTATGTTTAATTCAAATTATATTCAGGAATTTTGCCTGGCTTTTGTTAGAATGCGGAAAGATGCGTTGAACGAGTATCTGTTCATTCTCGAAATGGAGAAGTTTAGCATTGGAGATTTGCTGACCATGGAATGGAGCGACTTAAACGTGAAGATCAAGAAATGGGTTTGGCTTATTAAAATTGTTATTAGAGTCTACCTTACTAGTGAGCAAAGACTCTGTAACAATATATTTGAAGGCATTGGATCTTATAGTGCAGTCTGCTTTACTGAGATTTCTGCGACTTCTATGCTTCGCCTGTTGAATTTTGGAGAGGCCATAGCTATGGAACCCCATCGACCGGAAAAGCTGTTTCACTTGCTAGACATGTATGAGGTCCTAAAAAATCTTCTTGAATTTGTAGATGAATTGTTTTCCGAAGAAACAGAAAAGGGTTCTTTTCTCAAATTCGAGTTCCATAATCTTTTCAAGAAACTGGGTGTTTCTGCAAGGGCTACGTTTTTAGATTTTGGAAATTTCATTGCATGTTCCATATCAACAAACCCTTTTCCCGGCGGTGGTGTTCATCATCTAACAAGGTATGTAATGAACTACATCCATACTCTTACTGTTTTCAGAGACTCGCTTGTTTTCCTTCTCCAAGATCAAGCAACAGATGTCTTAAGCCCAACTACTGAGCTGCAGAGTGAAGTCAATAGCATACCTTGCCCAATGGCTTACCACCTTCAGTCAATCACTTCACATTTGCTATCCAATCTCAATAACAAATCCAAGTTGTACAAGGATGATGCTCTACGACATATTTTCTTAATGAATAACATCCATTATATAGTTCAAAAGGTTGAAAATTCTGATCTCATAGCTTTTCTTGGAAGTGGGTGGATGCGAGAACATATAAGAATGTTTCAAAGCCACGCAACTATCTATATGAGAGCCACCTGGCAGTCTGTTCTTTCTCTGCTAAGGCTTGATGGTGATGGAATGAAAACTTCAAAGGCAGTCTTTAAAGAAAAGTACAGGGCCTTCAATGCAGCCTTTGAGGAGATATATAAGAACCAAACTGGTTGGAATGTCCCAGATCCTCAACTACGTGATGACTTGCTTATTCAAACCTCAAATTGTGTAATTCAAGCATATCGAATCCTCTGCGGGTCAAGATCTCAATTTAACCGTGAAAAGTATATCAAATATACAACTGATGATCTGAGCAAGCATATGCTGGATCTCTTGCAAGGCTCATCGCGCTCGTTGCAAAGTTCACGCCGGAGATGA

Protein sequence

MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYTVGKLSELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDSQKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSLEVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNEYLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKGSFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSLVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDALRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDGDGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGSRSQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR
Homology
BLAST of Cucsat.G14657 vs. ExPASy Swiss-Prot
Match: Q9FNR3 (Exocyst complex component EXO70E2 OS=Arabidopsis thaliana OX=3702 GN=EXO70E2 PE=1 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 4.8e-141
Identity = 284/645 (44.03%), Postives = 403/645 (62.48%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTS-QNVNNELKKLLADLDSHLTSL----TSY 60
           M + +S +P S    H   A  H+VK LR S  N++  L+KLL+DL+ HL++     T  
Sbjct: 1   MAEFDSKVPVSGMNNHVFEACHHVVKALRASDNNLDANLRKLLSDLEMHLSTFGIADTKV 60

Query: 61  TVGKLSELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSAN 120
                SE++ RFK A ++I  WE+N+S ++++G  EA ++ +A+ ++  V  G ++L   
Sbjct: 61  EDAGFSEIKKRFKEAVKRIRSWETNQSTMFEAGLSEADQFFQALYDVQTVLVGFKALPMK 120

Query: 121 DSQKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVS 180
            +Q + ++  QA   + IAM RLE+EL  +L QHK + +PDY+   S   D+VY+ESFVS
Sbjct: 121 TNQMEKDVYNQATVALDIAMLRLEKELCDVLHQHKRHVQPDYLAVSSRRKDIVYDESFVS 180

Query: 181 AEDSLEVETS---HGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRK 240
            +D + VE S     E I   Y  +LV P V+ H+K+IAN MF   Y Q FC AF+ +++
Sbjct: 181 LDDEVIVEASSHEDDEQISDFYNSDLVDPIVLPHIKAIANAMFACEYDQPFCEAFIGVQR 240

Query: 241 DALNEYLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEG 300
           +AL EY+  LEME+FS  D+L M+W DLN  ++KW  ++KI+ +VYL SE++LC+ I   
Sbjct: 241 EALEEYMVTLEMERFSCVDVLRMDWEDLNGAMRKWTKVVKIITQVYLASEKQLCDQILGD 300

Query: 301 IGSYSAVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSE 360
             S S  CF EIS  ++L LLNFGEA+ +   +PE L   L MYEV   +L  VD LF +
Sbjct: 301 FESISTACFIEISKDAILSLLNFGEAVVLRSCKPEMLERFLSMYEVSAEILVDVDNLFPD 360

Query: 361 ETEKGSFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIH 420
           ET  GS L+  FHNL KKL      TFL F + IA   ST PF GGG+HHLTRYVMNY+ 
Sbjct: 361 ET--GSSLRIAFHNLSKKLADHTTTTFLKFKDAIASDESTRPFHGGGIHHLTRYVMNYLK 420

Query: 421 TLTVFRDSLVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLY 480
            L  + DSL  LLQ+   D   P    +  + S   PMA HL+SI + L S+L  K++LY
Sbjct: 421 LLPEYTDSLNSLLQNIHVDDSIPEKTGEDVLPSTFSPMARHLRSIVTTLESSLERKAQLY 480

Query: 481 KDDALRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSL 540
            D+AL+ IFLMNN  Y+VQKV+ S+L    G  W+R+HI  +Q + T Y R+TW S+L+L
Sbjct: 481 ADEALKSIFLMNNFRYMVQKVKGSELRRLFGDEWIRKHIASYQCNVTNYERSTWSSILAL 540

Query: 541 LRLDGDGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYR 600
           LR + D ++T     +E+ R F+ AF+++YKNQT W+VPD +LRDDL I TS  V+Q+YR
Sbjct: 541 LRDNNDSVRT----LRERCRLFSLAFDDVYKNQTRWSVPDSELRDDLHISTSVKVVQSYR 600

Query: 601 ILCGSRSQFNREKYIKYTTDDLSKHMLDLLQ--GSSRSLQSSRRR 636
              G  +    EK+I+YT +D+   +LDL +   S RSL+SSR+R
Sbjct: 601 GFLGRNAVRIGEKHIRYTCEDIENMLLDLFECLPSPRSLRSSRKR 639

BLAST of Cucsat.G14657 vs. ExPASy Swiss-Prot
Match: Q9LZD3 (Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.1e-65
Identity = 191/641 (29.80%), Postives = 313/641 (48.83%), Query Frame = 0

Query: 28  LRTSQNVNNELKKLLADLDSHLTSLTSYTVGKLSELETR-FKSAKEKITRWESNKSMIWD 87
           L+ SQ + + +  +L   DS L++L   T  + +++ T   + A E I R      +I  
Sbjct: 21  LQKSQTITDNVVSILGSFDSRLSALE--TAMRPTQIRTHAIRKAHENIDRTLKAAEVILS 80

Query: 88  -------------SGPKEASE-YMKAVDEIHKVQEGLRSLSANDSQKQNE-LLFQADSVV 147
                         GP E  E Y+ A+ ++ K+   +R   +N S K ++ +L  A+S++
Sbjct: 81  QFDLLRQAETKVLKGPHEDLESYLDAIAQLRKI---IRYFMSNKSFKSSDGVLNHANSLL 140

Query: 148 QIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSLEVETSHG---- 207
             A ++LE+E   +LA +    EPD +      +D + N S   + D       HG    
Sbjct: 141 AKAQSKLEEEFKQLLASYSKAVEPDRL------FDGLPN-SLRPSSDGDGGGKPHGGHHN 200

Query: 208 -ESIPALYAVNLVHP-HVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNEYLFILEME 267
            ++  A Y + ++ P  V+  L  +A  M  + + Q+    +   R   L E L  L +E
Sbjct: 201 DDAETAAYTLPILIPSRVLPLLHDLAQQMVQAGHQQQLLQIYRDTRSFVLEESLKKLGVE 260

Query: 268 KFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVCFTEIS 327
           K S  D+  M+W  L  KI  W+  ++I +++    E+++C+ IF G  S S  CF E++
Sbjct: 261 KLSKEDVQRMQWEVLEAKIGNWIHFMRIAVKLLFAGERQVCDQIFRGFDSLSDQCFAEVT 320

Query: 328 ATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKGSF---LKF 387
            +S+  LL+FG+AIA     PEKLF LLDMYE+++ L   ++ +F     KG     ++ 
Sbjct: 321 VSSVSMLLSFGDAIARSKRSPEKLFVLLDMYEIMRELHTEIETIF-----KGKACLEIRD 380

Query: 388 EFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSLV 447
               L K+L  +A+ TF DF   +    +      G VH LT YV+NY+  L  ++ +L 
Sbjct: 381 SATGLTKRLAQTAQETFGDFEEAVEKDATKTAVLDGTVHPLTSYVINYVKFLFDYQTTLK 440

Query: 448 FLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDALRHIFL 507
            L  +          +  S++ S+   +   LQ       +NL+ KSK YKD AL H+FL
Sbjct: 441 QLFLE-----FGNGDDSNSQLASVTMRIMQALQ-------NNLDGKSKQYKDPALTHLFL 500

Query: 508 MNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLD------ 567
           MNNIHY+V+ V  S+    LG  W++ H R+ Q HA  Y R  W  +L            
Sbjct: 501 MNNIHYMVRSVRRSEAKDLLGDDWVQRHRRIVQQHANQYKRVAWTKILQSSSAQGLTSSG 560

Query: 568 ------GDGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQA 627
                 G+    S+ + KE+++ FN  F+E+++ Q+ W VPD +LR+ L +  +  ++ A
Sbjct: 561 GGSLEGGNSSGVSRGLLKERFKMFNMQFDELHQRQSQWTVPDTELRESLRLAVAEVLLPA 620

BLAST of Cucsat.G14657 vs. ExPASy Swiss-Prot
Match: Q9FGH9 (Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.5e-65
Identity = 182/642 (28.35%), Postives = 322/642 (50.16%), Query Frame = 0

Query: 13  GEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLT--SLTSYTVGK----LSELETR 72
           GE+  LA  +H+ K L  ++++ +++ ++ ++ D   +   L     G+    ++ LE  
Sbjct: 5   GEEKLLAVARHIAKTLGHNESMADDILQIFSNFDGRFSREKLAEGQAGEDGSGVATLERA 64

Query: 73  FKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDSQKQNEL-LF 132
             S   +I+R+ +    IW + P +++ ++  +DE+  +   +R  S   S+K   + L 
Sbjct: 65  LNSIDGQISRFVAADQPIW-ADPADSAAFLDTIDELVAI---IREWSPMASEKPIGICLT 124

Query: 133 QADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVS-AEDSLEVET 192
           +AD ++Q AM R+E+E   ++ +    F          G     N  F S  E+  + + 
Sbjct: 125 RADDMMQQAMFRIEEEFRSLMERGAESF-----GLNPQGDAGAMNHRFDSEEEEDDDRDF 184

Query: 193 SHGESIPALYA---------VNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDAL 252
           ++G+ I    A         ++ +    I  L  +A  M  + + +     +   R++ L
Sbjct: 185 NNGDDIQIPVAQPLTDYDLIIDALPSATINDLHEMAKRMLGAGFGKACSHVYSSCRREFL 244

Query: 253 NEYLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGS 312
            E +  L ++K SI ++  M W +L  +I +W+    + +R+   SE+RLC+ +F G  S
Sbjct: 245 EESMSRLGLQKLSIEEVHKMPWQELEDEIDRWIKAANVALRILFPSERRLCDRVFFGFSS 304

Query: 313 YSAVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETE 372
            + + F E+   S ++LLNF +AIA+    PE+LF +LD++E +++L+   + +FS++  
Sbjct: 305 AADLSFMEVCRGSTIQLLNFADAIAIGSRSPERLFKVLDVFETMRDLMPEFESVFSDQF- 364

Query: 373 KGSFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLT 432
             S L+ E   ++K+LG + R  F++  N I    +    PGGG+H +TRYVMNY+    
Sbjct: 365 -CSVLRNEAVTIWKRLGEAIRGIFMELENLIRRDPAKAAVPGGGLHPITRYVMNYLRAAC 424

Query: 433 VFRDSLVFLLQDQATDVLSPTTELQSEVNSIPCP----MAYHLQSITSHLLSNLNNKSKL 492
             R +L                ++  E N +P      +   +  I   L SNL  KSK+
Sbjct: 425 RSRQTL---------------EQVFEESNGVPSKDSTLLTVQMSWIMELLESNLEVKSKV 484

Query: 493 YKDDALRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLS 552
           YKD AL ++FLMNN  YIVQKV++ DL   LG  W+R+H    + +   Y R++W  +L 
Sbjct: 485 YKDPALCYVFLMNNGRYIVQKVKDGDLGLLLGDDWIRKHNVKVKQYHMNYQRSSWNKMLG 544

Query: 553 LLRLDGD--GMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQ 612
           LL++D    GM       KEK + FN  F+EI K  + W V D QL+++L I  +  ++ 
Sbjct: 545 LLKVDNTAAGMNGLGKTMKEKLKQFNIQFDEICKVHSTWVVFDEQLKEELKISLARLLVP 604

Query: 613 AYRILCGSRSQF-----NREKYIKYTTDDLSKHMLDLLQGSS 627
           AY    G          N +KYIKY  +D+   + +L +G++
Sbjct: 605 AYGSFIGRFQNLGDIGKNADKYIKYGVEDIEARINELFKGTT 620

BLAST of Cucsat.G14657 vs. ExPASy Swiss-Prot
Match: Q9LMJ4 (Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.5e-54
Identity = 162/571 (28.37%), Postives = 276/571 (48.34%), Query Frame = 0

Query: 69  SAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDSQKQNELLFQAD 128
           S + +I+ +      IW S P ++  ++ +VDE+  +   LRS++ + S    +   +AD
Sbjct: 66  SLERQISSYIVEDRPIW-SDPVDSRTFLDSVDELLAIAGDLRSMAGDKSVAVCQ--SRAD 125

Query: 129 SVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSLEVETSHGE 188
            ++Q  M RL++E   ++ +    F+ D              + F   ED+   +TS G 
Sbjct: 126 ELIQQVMFRLQEEFGFVMDRAPDSFDSD--------------DEFPGEEDN---DTSDGV 185

Query: 189 SIPA-----LYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNEYLFILE 248
            +          +  +   VI  L +IA  M    + +E    +   R++ L E L  L 
Sbjct: 186 IVARPITDYKIVIEALQSSVIGDLNAIAVRMVAGGFAKECSRVYSSRRREFLEESLSRLH 245

Query: 249 MEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEG--IGSYSAVCF 308
           +   S+ ++    W DL  +I +W+  + ++  V+  SE+ LC+ +F    + S + + F
Sbjct: 246 LRGLSMEEVQESPWQDLEDEIDRWIKAVTLIFHVFFPSERLLCDRVFSDLPVSSVTDLSF 305

Query: 309 TEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKGSFLK 368
            E+   +  +LLNF +AIA+    PE+LF ++D+YE +++L+  ++ LFS+     S L+
Sbjct: 306 MEVCRGTTTQLLNFADAIALGSRLPERLFKVVDLYEAMQDLIPKMETLFSD--RYCSPLR 365

Query: 369 FEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSL 428
            E   + K+LG + R  F++  N I        FPGGG+H +TRYVMNY+      R SL
Sbjct: 366 HEALAIHKRLGEAIRGIFMELENLIRRDPPKTAFPGGGIHPITRYVMNYLRAACKSRQSL 425

Query: 429 VFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDALRHIF 488
             +L     +  S T            P++  +  +   L SNL  K + Y+D +L  +F
Sbjct: 426 EQILDQTGNETGSDTR-----------PLSVQIIWVLELLESNLEGKKRTYRDPSLCFLF 485

Query: 489 LMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDGDGMK 548
           +MNN  YI+ K ++++L   LG  W+ +H    + + + Y R++W  V+ LLR DG   K
Sbjct: 486 MMNNDKYILDKAKDNELGLVLGEDWIVKHAAKLRQYHSNYRRSSWNQVVGLLRTDGPYPK 545

Query: 549 TSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAY----RILCGS 608
                  E  R F + F+E+ K Q+ W V D QLR++L    +  V  AY    R L  S
Sbjct: 546 -----LVENLRLFKSQFDEVCKVQSQWVVSDGQLREELRSSVAGIVSPAYSNFIRRLKES 598

Query: 609 RSQFNR--EKYIKYTTDDLSKHMLDLLQGSS 627
                R  E +I YT +D+   +  L + SS
Sbjct: 606 PEINGRRGEPFIPYTVEDVEFIIKRLFKESS 598

BLAST of Cucsat.G14657 vs. ExPASy Swiss-Prot
Match: Q8VY27 (Exocyst complex component EXO70H1 OS=Arabidopsis thaliana OX=3702 GN=EXO70H1 PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 4.1e-52
Identity = 165/607 (27.18%), Postives = 289/607 (47.61%), Query Frame = 0

Query: 50  TSLTSYTVGKLSE------LETRFKSAKEKITRWESNKS------MIWDSGPKEASEYMK 109
           +S TS+    L++      +E   ++A   I +W+ N         +++   KEA E+++
Sbjct: 23  SSFTSFPASPLNQNFTQAMMEETVETADSVIRKWDPNTPSFTKIVSLFNHSRKEAKEFIR 82

Query: 110 AVDEIHKVQEGLRSLSANDSQKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDY 169
            V ++ +    +  L + DSQ     L  A +++QIAMARLE+E   IL+ ++   +P+ 
Sbjct: 83  CVRDLRR---AMHFLVSQDSQSPK--LALAQTLMQIAMARLEKEFFQILSSNRDKLDPES 142

Query: 170 VPFQSGGYDVVYNESFVSAEDSLEVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNS 229
           V  QS        E  + ++D  E +    ++  ++  V      V++ LK+IA  M + 
Sbjct: 143 VSGQSSISSNSEFEDVMGSDDDDESDNEMRKAGESITQVEKASAMVMSDLKAIAESMISC 202

Query: 230 NYIQEFCLAFVRMRKDALNEYLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRV 289
            Y +E    + R+RK  ++E L +L +E +        +W  L   IK W+   KI I  
Sbjct: 203 GYGKECVKIYKRVRKSIVDEGLSLLGIEIYKGSRFHRTDWVTLEHMIKNWIKAAKIGIAT 262

Query: 290 YLTSEQRLCNNIFEGIGSYSAVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYE 349
               E+ LC+++F    S    CF EI+  +   L  F E +A E    E++F L+D+  
Sbjct: 263 LFRGEKLLCDHVFSASNSTRESCFYEIANEAATNLFKFPEFVAKEKKSHERIFPLMDLQA 322

Query: 350 VLKNLLEFVDELFSEETEKGSFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPG 409
            + +L + ++ +F  +   G  +K +     +KL VS  +   DF + I    +    PG
Sbjct: 323 AISDLWQDIEMIFHFDAVAG--VKSQALTSLQKLKVSIHSALTDFESIIQKDTTKALTPG 382

Query: 410 GGVHHLTRYVMNYIHTLTVFRDSLVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSI 469
           GG+H LTR  MN+I +L+ +   L  +L D    +   T  L+S V +       H  ++
Sbjct: 383 GGIHKLTRSTMNFISSLSKYSGVLSEILADH--PLPRNTRLLESYVRAPISEDEQHNHAL 442

Query: 470 TSH-------LLSNLNNKSKLYKDDALRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREH 529
           + H       LL  L+ K++ YKD +L ++FL NN+  I++ V ++ L   LG  W+ +H
Sbjct: 443 SVHFAWLILVLLCKLDTKAEHYKDVSLSYLFLANNLQIIIETVGSTPLRNLLGDDWLNKH 502

Query: 530 IRMFQSHATIYMRATWQSVLSLLRLDGDGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNV 589
                ++A  Y  A W +V   L  +   +   +A  K  +R F+ AFEE Y  Q+   V
Sbjct: 503 EDKLCAYAGNYEIAAWSNVFMSLPEEPTDLSPEEA--KIYFRRFHTAFEEAYMKQSSRVV 562

Query: 590 PDPQLRDDLLIQTSNCVIQAYR-----ILCGSRSQFNREKYIKYTTDDLSKHMLDLLQGS 633
           P+ +LRD+L +  +  ++  YR      L     + N E  +++  D+L  ++ DL  G+
Sbjct: 563 PNAKLRDELKVSIAKKLVPEYREFYRKYLPMLGQERNIEILVRFKPDNLENYISDLFHGT 618

BLAST of Cucsat.G14657 vs. NCBI nr
Match: XP_004137600.1 (exocyst complex component EXO70E2 [Cucumis sativus] >XP_031744501.1 exocyst complex component EXO70E2 [Cucumis sativus] >XP_031744503.1 exocyst complex component EXO70E2 [Cucumis sativus] >XP_031744509.1 exocyst complex component EXO70E2 [Cucumis sativus] >KGN64059.2 hypothetical protein Csa_014168 [Cucumis sativus])

HSP 1 Score: 1253 bits (3242), Expect = 0.0
Identity = 634/635 (99.84%), Postives = 634/635 (99.84%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYTVGKL 60
           MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYTVGKL
Sbjct: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYTVGKL 60

Query: 61  SELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDSQKQ 120
           SELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDSQKQ
Sbjct: 61  SELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDSQKQ 120

Query: 121 NELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSL 180
           NELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSL
Sbjct: 121 NELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSL 180

Query: 181 EVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNEYLF 240
           EVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNEYLF
Sbjct: 181 EVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNEYLF 240

Query: 241 ILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVC 300
           ILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVC
Sbjct: 241 ILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVC 300

Query: 301 FTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKGSFL 360
           FTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLL FVDELFSEETEKGSFL
Sbjct: 301 FTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGFVDELFSEETEKGSFL 360

Query: 361 KFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDS 420
           KFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDS
Sbjct: 361 KFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDS 420

Query: 421 LVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDALRHI 480
           LVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDALRHI
Sbjct: 421 LVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDALRHI 480

Query: 481 FLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDGDGM 540
           FLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDGDGM
Sbjct: 481 FLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDGDGM 540

Query: 541 KTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGSRSQ 600
           KTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGSRSQ
Sbjct: 541 KTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGSRSQ 600

Query: 601 FNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635
           FNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR
Sbjct: 601 FNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635

BLAST of Cucsat.G14657 vs. NCBI nr
Match: XP_008450312.1 (PREDICTED: exocyst complex component EXO70A1 [Cucumis melo] >XP_008450459.1 PREDICTED: exocyst complex component EXO70A1 [Cucumis melo] >XP_016899546.1 PREDICTED: exocyst complex component EXO70A1 [Cucumis melo] >XP_016899547.1 PREDICTED: exocyst complex component EXO70A1 [Cucumis melo] >XP_016899548.1 PREDICTED: exocyst complex component EXO70A1 [Cucumis melo])

HSP 1 Score: 1174 bits (3037), Expect = 0.0
Identity = 594/638 (93.10%), Postives = 611/638 (95.77%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYT---V 60
           MGDCESI+PTSEGEQHALAAFQHLVKFLR SQNVNNELKKLLADLDSHLTSL  YT   V
Sbjct: 1   MGDCESIMPTSEGEQHALAAFQHLVKFLRKSQNVNNELKKLLADLDSHLTSLPLYTHLEV 60

Query: 61  GKLSELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDS 120
           GKLSELETRF SA+EKI RWESNKSMIWDSGPKEA EYMKAVDEIHKVQEGLRSLSANDS
Sbjct: 61  GKLSELETRFISAEEKIMRWESNKSMIWDSGPKEACEYMKAVDEIHKVQEGLRSLSANDS 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180
           QKQNELLFQADSVVQIAMARLEQELIHIL QHKLYFEPDYVPFQSGGYDVVYNESFVSAE
Sbjct: 121 QKQNELLFQADSVVQIAMARLEQELIHILVQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180

Query: 181 DSLEVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNE 240
           DSLEVETSHGESIPA+YAVNLVHPHVI H+KSI N+MF SNYIQEFCLAFV+MRKDALNE
Sbjct: 181 DSLEVETSHGESIPAIYAVNLVHPHVIPHIKSIGNLMFTSNYIQEFCLAFVKMRKDALNE 240

Query: 241 YLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300
           YLFILEMEK+SIGDLL+MEW DLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS
Sbjct: 241 YLFILEMEKYSIGDLLSMEWGDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKG 360
           AVCFTEISAT+MLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLL ++DELFSEETEKG
Sbjct: 301 AVCFTEISATAMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGYIDELFSEETEKG 360

Query: 361 SFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
            FLK EFHNLFKKLG SARATF+DFGNFIACSISTNPFPGGGVHHLTRYVMNYI TLTVF
Sbjct: 361 CFLKVEFHNLFKKLGDSARATFVDFGNFIACSISTNPFPGGGVHHLTRYVMNYIKTLTVF 420

Query: 421 RDSLVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDAL 480
           RDSLVFLLQDQATD LSPT+ELQSE+NSIPCPMAYHLQSITSHL+SNLNNKSKLYKDDAL
Sbjct: 421 RDSLVFLLQDQATDGLSPTSELQSEINSIPCPMAYHLQSITSHLISNLNNKSKLYKDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDG 540
           RHIFLMNNIHYIVQKVENS+L+AFLGSGWMREHIRMFQSHA IYMR TWQSVLSLLRLDG
Sbjct: 481 RHIFLMNNIHYIVQKVENSELVAFLGSGWMREHIRMFQSHAIIYMRVTWQSVLSLLRLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           DGMKT KAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRIL  S
Sbjct: 541 DGMKTPKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILYES 600

Query: 601 RSQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635
           RSQ NREKYIKYTTDDLSKHMLDLLQGSS SLQSSRRR
Sbjct: 601 RSQSNREKYIKYTTDDLSKHMLDLLQGSSCSLQSSRRR 638

BLAST of Cucsat.G14657 vs. NCBI nr
Match: TYJ98937.1 (exocyst complex component EXO70A1 [Cucumis melo var. makuwa])

HSP 1 Score: 1159 bits (2998), Expect = 0.0
Identity = 587/631 (93.03%), Postives = 604/631 (95.72%), Query Frame = 0

Query: 8   IPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYT---VGKLSELE 67
           +PTSEGEQHALAAFQHLVKFLR SQNVNNELKKLLADLDSHLTSL  YT   VGKLSELE
Sbjct: 1   MPTSEGEQHALAAFQHLVKFLRKSQNVNNELKKLLADLDSHLTSLPLYTHLEVGKLSELE 60

Query: 68  TRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDSQKQNELL 127
           TRF SA+EKI RWESNKSMIWDSGPKEA EYMKAVDEIHKVQEGLRSLSANDSQKQNELL
Sbjct: 61  TRFISAEEKIMRWESNKSMIWDSGPKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNELL 120

Query: 128 FQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSLEVET 187
           FQADSVVQIAMARLEQELIHIL QHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSLEVET
Sbjct: 121 FQADSVVQIAMARLEQELIHILVQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSLEVET 180

Query: 188 SHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNEYLFILEM 247
           SHGESIPA+YAVNLVHPHVI H+KSI N+MF SNYIQEFCLAFV+MRKDALNEYLFILEM
Sbjct: 181 SHGESIPAIYAVNLVHPHVIPHIKSIGNLMFTSNYIQEFCLAFVKMRKDALNEYLFILEM 240

Query: 248 EKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVCFTEI 307
           EK+SIGDLL+MEW DLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVCFTEI
Sbjct: 241 EKYSIGDLLSMEWGDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVCFTEI 300

Query: 308 SATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKGSFLKFEF 367
           SAT+MLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLL ++DELFSEETEKG FLK EF
Sbjct: 301 SATAMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGYIDELFSEETEKGCFLKVEF 360

Query: 368 HNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSLVFL 427
           HNLFKKLG SARATF+DFGNFIACSISTNPFPGGGVHHLTRYVMNYI TLTVFRDSLVFL
Sbjct: 361 HNLFKKLGDSARATFVDFGNFIACSISTNPFPGGGVHHLTRYVMNYIKTLTVFRDSLVFL 420

Query: 428 LQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDALRHIFLMN 487
           LQDQATD LSPT+ELQSE+NSIPCPMAYHLQSITSHL+SNLNNKSKLYKDDALRHIFLMN
Sbjct: 421 LQDQATDGLSPTSELQSEINSIPCPMAYHLQSITSHLISNLNNKSKLYKDDALRHIFLMN 480

Query: 488 NIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDGDGMKTSK 547
           NIHYIVQKVENS+L+AFLGSGWMREHIRMFQSHA IYMR TWQSVLSLLRLDGDGMKT K
Sbjct: 481 NIHYIVQKVENSELVAFLGSGWMREHIRMFQSHAIIYMRVTWQSVLSLLRLDGDGMKTPK 540

Query: 548 AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGSRSQFNRE 607
           AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRIL  SRSQ NRE
Sbjct: 541 AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILYESRSQSNRE 600

Query: 608 KYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635
           KYIKYTTDDLSKHMLDLLQGSS SLQSSRRR
Sbjct: 601 KYIKYTTDDLSKHMLDLLQGSSCSLQSSRRR 631

BLAST of Cucsat.G14657 vs. NCBI nr
Match: XP_038893966.1 (exocyst complex component EXO70E2 [Benincasa hispida])

HSP 1 Score: 1120 bits (2896), Expect = 0.0
Identity = 564/638 (88.40%), Postives = 594/638 (93.10%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYT---V 60
           MGDCESIIPTSEGEQH LA+FQHLVKFLRTSQNVN+ELK+LLADLDSHL ++T YT   V
Sbjct: 1   MGDCESIIPTSEGEQHVLASFQHLVKFLRTSQNVNDELKRLLADLDSHLNTMTLYTHLEV 60

Query: 61  GKLSELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDS 120
           GKLSELETR KSA+EKI RWESNKSMIWDS  KEA EYMKAVDEIH VQEGLRSLSANDS
Sbjct: 61  GKLSELETRLKSAEEKIRRWESNKSMIWDSCSKEACEYMKAVDEIHMVQEGLRSLSANDS 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180
           QKQNELLFQADSVVQIAMARLEQEL+HILAQHKLYFEPDYVPFQSGGYDVVYNESFVS E
Sbjct: 121 QKQNELLFQADSVVQIAMARLEQELVHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSTE 180

Query: 181 DSLEVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNE 240
           DS+E ETSH ESIPA+Y VNLVHPHVI HLKSIANVMF SNYIQEFCLAFV+MRKDALNE
Sbjct: 181 DSMEEETSHRESIPAVYVVNLVHPHVIPHLKSIANVMFTSNYIQEFCLAFVKMRKDALNE 240

Query: 241 YLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300
           YLFILEMEK+SI DLLTMEW  LNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGI SYS
Sbjct: 241 YLFILEMEKYSIADLLTMEWGVLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIESYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKG 360
           AVCFTEISATSML LLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLL ++D LFSEETEKG
Sbjct: 301 AVCFTEISATSMLHLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGYIDALFSEETEKG 360

Query: 361 SFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
           SFLK EFHNLFKKLG SARATF+DFG+FIACSISTNPFPGGGVHHLTRYVMN+I TLTV+
Sbjct: 361 SFLKVEFHNLFKKLGDSARATFVDFGSFIACSISTNPFPGGGVHHLTRYVMNFIKTLTVY 420

Query: 421 RDSLVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDAL 480
           RDSLVFLLQD ATD LSP +ELQ+E+NS+PCPMAYH QSITSHL+SNLNNKSKLYKDDAL
Sbjct: 421 RDSLVFLLQDNATDGLSPASELQNEINSMPCPMAYHFQSITSHLISNLNNKSKLYKDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDG 540
           RHIFLMNNIHY+VQKVE+S+L AFLGSGWMREH RMFQ+H TIY+R TW SVLSLLRLDG
Sbjct: 481 RHIFLMNNIHYMVQKVEDSELKAFLGSGWMREHTRMFQNHGTIYVRVTWHSVLSLLRLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           DG+ T KAVFKEKYRAFNAAFEEIYKNQTGW VPDP+L DDLLIQTSNCVIQAYRILC S
Sbjct: 541 DGLNTPKAVFKEKYRAFNAAFEEIYKNQTGWIVPDPRLCDDLLIQTSNCVIQAYRILCES 600

Query: 601 RSQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635
           RSQ+NREKYIKYTTDDLSKHMLDL QGS RSLQSSRRR
Sbjct: 601 RSQYNREKYIKYTTDDLSKHMLDLFQGSPRSLQSSRRR 638

BLAST of Cucsat.G14657 vs. NCBI nr
Match: XP_023520029.1 (exocyst complex component EXO70E2 [Cucurbita pepo subsp. pepo] >XP_023520030.1 exocyst complex component EXO70E2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 997 bits (2577), Expect = 0.0
Identity = 505/638 (79.15%), Postives = 551/638 (86.36%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYTV--- 60
           MGDCESIIPTSEGEQH +AAFQHL K LRTSQNVN+ELK+LL DLDSHLTS+T YT    
Sbjct: 1   MGDCESIIPTSEGEQHVIAAFQHLAKLLRTSQNVNDELKRLLTDLDSHLTSMTLYTHLED 60

Query: 61  GKLSELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDS 120
           GKL+ELE+RFKSA+EKI RWESN+SMIWDSGPKEA EYMKAVDEIHKVQEGL SL  ND 
Sbjct: 61  GKLNELESRFKSAEEKIRRWESNESMIWDSGPKEACEYMKAVDEIHKVQEGLISLPVNDG 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180
           +KQ +LL QA+SV+Q AMARLEQEL+HILAQHKLYFEP+ VPFQSGGYDVVYNESF S E
Sbjct: 121 RKQKDLLCQAESVIQFAMARLEQELVHILAQHKLYFEPNCVPFQSGGYDVVYNESFASVE 180

Query: 181 DSLEVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNE 240
           DSLE ETS  ESIPA+  VN+VHPHVI HLKSIAN MF SNYIQEF LAFVRMRKDAL+E
Sbjct: 181 DSLEEETSQRESIPAVCIVNMVHPHVIPHLKSIANAMFTSNYIQEFSLAFVRMRKDALDE 240

Query: 241 YLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300
           YL ILEME+ SIGDL TMEW  LNVKIKKW+WLIK+VIRVYL SEQRLCNNIFEG GSYS
Sbjct: 241 YLLILEMERHSIGDLQTMEWDVLNVKIKKWIWLIKVVIRVYLASEQRLCNNIFEGFGSYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKG 360
           A CF EISATSML LLNFGEAIAM   RPEKLF LLDMYEVL+NLL  +  LFSE+TE G
Sbjct: 301 ATCFAEISATSMLCLLNFGEAIAMTTRRPEKLFRLLDMYEVLENLLGDIGALFSEDTEMG 360

Query: 361 SFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
           SFLK EFHNLF KL  SARATF+DFG+ IA SIST+PFPGGGVHHLT+YVMNYI TLTV+
Sbjct: 361 SFLKAEFHNLFMKLSDSARATFVDFGSLIARSISTSPFPGGGVHHLTKYVMNYIRTLTVY 420

Query: 421 RDSLVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDAL 480
           RDSLVFLL+D+A D L P +EL +E+NS+PCP+A+HLQSITS L+SNLNNKSKLY DDAL
Sbjct: 421 RDSLVFLLRDEAADGLGPASELLNEINSMPCPLAFHLQSITSQLISNLNNKSKLYNDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDG 540
           RHIFLMNNIHY+VQKV+ S+L AF G  W+REH RM Q HA IYMR TWQSV+SLL+LDG
Sbjct: 481 RHIFLMNNIHYMVQKVKGSELRAFFGGEWIREHNRMLQIHANIYMRVTWQSVISLLKLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           DG  T KAVFKEKYRAFN AFEEIYKNQTGWNV DP+LRDDL I TSNCVIQAYR LC  
Sbjct: 541 DGTNTPKAVFKEKYRAFNVAFEEIYKNQTGWNVSDPELRDDLHILTSNCVIQAYRFLCNL 600

Query: 601 RSQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635
           +SQFN EKYIKYT DDLSKH+LDL QGS RSLQSSRRR
Sbjct: 601 KSQFNCEKYIKYTIDDLSKHILDLFQGSPRSLQSSRRR 638

BLAST of Cucsat.G14657 vs. ExPASy TrEMBL
Match: A0A1S4DU88 (Exocyst subunit Exo70 family protein OS=Cucumis melo OX=3656 GN=LOC103491934 PE=3 SV=1)

HSP 1 Score: 1174 bits (3037), Expect = 0.0
Identity = 594/638 (93.10%), Postives = 611/638 (95.77%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYT---V 60
           MGDCESI+PTSEGEQHALAAFQHLVKFLR SQNVNNELKKLLADLDSHLTSL  YT   V
Sbjct: 1   MGDCESIMPTSEGEQHALAAFQHLVKFLRKSQNVNNELKKLLADLDSHLTSLPLYTHLEV 60

Query: 61  GKLSELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDS 120
           GKLSELETRF SA+EKI RWESNKSMIWDSGPKEA EYMKAVDEIHKVQEGLRSLSANDS
Sbjct: 61  GKLSELETRFISAEEKIMRWESNKSMIWDSGPKEACEYMKAVDEIHKVQEGLRSLSANDS 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180
           QKQNELLFQADSVVQIAMARLEQELIHIL QHKLYFEPDYVPFQSGGYDVVYNESFVSAE
Sbjct: 121 QKQNELLFQADSVVQIAMARLEQELIHILVQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180

Query: 181 DSLEVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNE 240
           DSLEVETSHGESIPA+YAVNLVHPHVI H+KSI N+MF SNYIQEFCLAFV+MRKDALNE
Sbjct: 181 DSLEVETSHGESIPAIYAVNLVHPHVIPHIKSIGNLMFTSNYIQEFCLAFVKMRKDALNE 240

Query: 241 YLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300
           YLFILEMEK+SIGDLL+MEW DLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS
Sbjct: 241 YLFILEMEKYSIGDLLSMEWGDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKG 360
           AVCFTEISAT+MLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLL ++DELFSEETEKG
Sbjct: 301 AVCFTEISATAMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGYIDELFSEETEKG 360

Query: 361 SFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
            FLK EFHNLFKKLG SARATF+DFGNFIACSISTNPFPGGGVHHLTRYVMNYI TLTVF
Sbjct: 361 CFLKVEFHNLFKKLGDSARATFVDFGNFIACSISTNPFPGGGVHHLTRYVMNYIKTLTVF 420

Query: 421 RDSLVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDAL 480
           RDSLVFLLQDQATD LSPT+ELQSE+NSIPCPMAYHLQSITSHL+SNLNNKSKLYKDDAL
Sbjct: 421 RDSLVFLLQDQATDGLSPTSELQSEINSIPCPMAYHLQSITSHLISNLNNKSKLYKDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDG 540
           RHIFLMNNIHYIVQKVENS+L+AFLGSGWMREHIRMFQSHA IYMR TWQSVLSLLRLDG
Sbjct: 481 RHIFLMNNIHYIVQKVENSELVAFLGSGWMREHIRMFQSHAIIYMRVTWQSVLSLLRLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           DGMKT KAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRIL  S
Sbjct: 541 DGMKTPKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILYES 600

Query: 601 RSQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635
           RSQ NREKYIKYTTDDLSKHMLDLLQGSS SLQSSRRR
Sbjct: 601 RSQSNREKYIKYTTDDLSKHMLDLLQGSSCSLQSSRRR 638

BLAST of Cucsat.G14657 vs. ExPASy TrEMBL
Match: A0A5D3BIJ1 (Exocyst subunit Exo70 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001380 PE=3 SV=1)

HSP 1 Score: 1159 bits (2998), Expect = 0.0
Identity = 587/631 (93.03%), Postives = 604/631 (95.72%), Query Frame = 0

Query: 8   IPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYT---VGKLSELE 67
           +PTSEGEQHALAAFQHLVKFLR SQNVNNELKKLLADLDSHLTSL  YT   VGKLSELE
Sbjct: 1   MPTSEGEQHALAAFQHLVKFLRKSQNVNNELKKLLADLDSHLTSLPLYTHLEVGKLSELE 60

Query: 68  TRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDSQKQNELL 127
           TRF SA+EKI RWESNKSMIWDSGPKEA EYMKAVDEIHKVQEGLRSLSANDSQKQNELL
Sbjct: 61  TRFISAEEKIMRWESNKSMIWDSGPKEACEYMKAVDEIHKVQEGLRSLSANDSQKQNELL 120

Query: 128 FQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSLEVET 187
           FQADSVVQIAMARLEQELIHIL QHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSLEVET
Sbjct: 121 FQADSVVQIAMARLEQELIHILVQHKLYFEPDYVPFQSGGYDVVYNESFVSAEDSLEVET 180

Query: 188 SHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNEYLFILEM 247
           SHGESIPA+YAVNLVHPHVI H+KSI N+MF SNYIQEFCLAFV+MRKDALNEYLFILEM
Sbjct: 181 SHGESIPAIYAVNLVHPHVIPHIKSIGNLMFTSNYIQEFCLAFVKMRKDALNEYLFILEM 240

Query: 248 EKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVCFTEI 307
           EK+SIGDLL+MEW DLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVCFTEI
Sbjct: 241 EKYSIGDLLSMEWGDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYSAVCFTEI 300

Query: 308 SATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKGSFLKFEF 367
           SAT+MLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLL ++DELFSEETEKG FLK EF
Sbjct: 301 SATAMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLGYIDELFSEETEKGCFLKVEF 360

Query: 368 HNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVFRDSLVFL 427
           HNLFKKLG SARATF+DFGNFIACSISTNPFPGGGVHHLTRYVMNYI TLTVFRDSLVFL
Sbjct: 361 HNLFKKLGDSARATFVDFGNFIACSISTNPFPGGGVHHLTRYVMNYIKTLTVFRDSLVFL 420

Query: 428 LQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDALRHIFLMN 487
           LQDQATD LSPT+ELQSE+NSIPCPMAYHLQSITSHL+SNLNNKSKLYKDDALRHIFLMN
Sbjct: 421 LQDQATDGLSPTSELQSEINSIPCPMAYHLQSITSHLISNLNNKSKLYKDDALRHIFLMN 480

Query: 488 NIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDGDGMKTSK 547
           NIHYIVQKVENS+L+AFLGSGWMREHIRMFQSHA IYMR TWQSVLSLLRLDGDGMKT K
Sbjct: 481 NIHYIVQKVENSELVAFLGSGWMREHIRMFQSHAIIYMRVTWQSVLSLLRLDGDGMKTPK 540

Query: 548 AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGSRSQFNRE 607
           AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRIL  SRSQ NRE
Sbjct: 541 AVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILYESRSQSNRE 600

Query: 608 KYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635
           KYIKYTTDDLSKHMLDLLQGSS SLQSSRRR
Sbjct: 601 KYIKYTTDDLSKHMLDLLQGSSCSLQSSRRR 631

BLAST of Cucsat.G14657 vs. ExPASy TrEMBL
Match: A0A6J1KLM6 (Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111495300 PE=3 SV=1)

HSP 1 Score: 984 bits (2544), Expect = 0.0
Identity = 499/638 (78.21%), Postives = 547/638 (85.74%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYTV--- 60
           MGDCESIIPTSEGEQH +AAFQHL K LRTSQNVN+ELK+LL DLDSHLTS+T YT    
Sbjct: 1   MGDCESIIPTSEGEQHVIAAFQHLAKLLRTSQNVNDELKRLLTDLDSHLTSMTLYTHLQD 60

Query: 61  GKLSELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDS 120
           GKL+ELE+RFKSA+EKI RWESN+SMIWDSGPKEA EYMKAVDEIHKVQEGL SL  ND 
Sbjct: 61  GKLNELESRFKSAEEKIRRWESNESMIWDSGPKEACEYMKAVDEIHKVQEGLISLPVNDG 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180
           +KQ +LL QA+SV+Q AMARLEQEL+HILAQ+KLYFEP+ VPFQSGGYD VYNESF S E
Sbjct: 121 RKQKDLLCQAESVIQFAMARLEQELVHILAQNKLYFEPNCVPFQSGGYDAVYNESFASVE 180

Query: 181 DSLEVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNE 240
           DSLE ETS  ESIPA+  VN+VHPHVI HLKSIAN MF SNYIQEF LAFV MRKDAL+E
Sbjct: 181 DSLEEETSQRESIPAVCIVNMVHPHVIPHLKSIANAMFTSNYIQEFSLAFVIMRKDALDE 240

Query: 241 YLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300
           YL ILEME+ SIGDL TMEW  LNVKIKKW+WLIK+VIRVYL SEQRLCNNIFEG GSYS
Sbjct: 241 YLLILEMERHSIGDLQTMEWDVLNVKIKKWIWLIKVVIRVYLASEQRLCNNIFEGFGSYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKG 360
           A CF EISATSML LLNFGEAIAM   RPEKLF LLDMYEVL+NLL  +  LFSE+TE G
Sbjct: 301 ATCFAEISATSMLCLLNFGEAIAMTTRRPEKLFRLLDMYEVLENLLVDIGALFSEDTEMG 360

Query: 361 SFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
           SFLK EFHNLF KL  SARATF+DFG+ IA SIST+PFPGGGVHHLT+YVMNY  TLTV+
Sbjct: 361 SFLKAEFHNLFMKLSDSARATFVDFGSLIARSISTSPFPGGGVHHLTKYVMNYTRTLTVY 420

Query: 421 RDSLVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDAL 480
           RDSLVFLL+D+A D L P +EL +E+NS+PCP+A+HLQSITS L+SNLNNKSKLY DDAL
Sbjct: 421 RDSLVFLLRDEAADSLGPASELLNEINSMPCPLAFHLQSITSQLISNLNNKSKLYNDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDG 540
           RHIFLMNNIHY++QKV+ S+L AF G  W+REH RM QSHA IYMR TWQSV+SLLRLDG
Sbjct: 481 RHIFLMNNIHYMIQKVKGSELRAFFGGEWIREHNRMLQSHANIYMRVTWQSVISLLRLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           D   T KAVFKEKYRAFN AFEEIYKNQTGWNV DP+LRDDL I TSNCVIQAYR LC  
Sbjct: 541 DETNTPKAVFKEKYRAFNVAFEEIYKNQTGWNVSDPELRDDLHILTSNCVIQAYRFLCNL 600

Query: 601 RSQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635
           +SQFN E+YIKYT DDLSKH+LDL QGS  SLQSSRRR
Sbjct: 601 KSQFNCERYIKYTIDDLSKHILDLFQGSPDSLQSSRRR 638

BLAST of Cucsat.G14657 vs. ExPASy TrEMBL
Match: A0A6J1E6T2 (Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC111431322 PE=3 SV=1)

HSP 1 Score: 982 bits (2539), Expect = 0.0
Identity = 499/638 (78.21%), Postives = 546/638 (85.58%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYTV--- 60
           MGDCESIIPTSEGEQH +AAFQHL K LRTSQNVN+ELK+LL DLDSHLTS+T YT    
Sbjct: 1   MGDCESIIPTSEGEQHVIAAFQHLAKLLRTSQNVNDELKRLLIDLDSHLTSMTLYTHLQD 60

Query: 61  GKLSELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDS 120
           GKL+ELE+RFKSA+EKI RWESN+SMIWDSGPKEA EYMKAVDEIHKVQEGL SL  ND 
Sbjct: 61  GKLNELESRFKSAEEKIRRWESNESMIWDSGPKEACEYMKAVDEIHKVQEGLISLPVNDG 120

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180
           +KQ +LL QA+SV+Q AM+RLEQEL+HILAQHKLYFEP+ VPFQSGGYDV+YNESF S E
Sbjct: 121 RKQKDLLSQAESVIQFAMSRLEQELVHILAQHKLYFEPNCVPFQSGGYDVLYNESFASLE 180

Query: 181 DSLEVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNE 240
           DSLE ETS  ESIPA+  VN+VHPHVI HLKSIAN MF SNYIQEF LAFVRMRKDAL+E
Sbjct: 181 DSLEEETSQRESIPAVCIVNMVHPHVIPHLKSIANAMFTSNYIQEFSLAFVRMRKDALDE 240

Query: 241 YLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300
           YL ILEME+ SIGDL TMEW  LNVKIKKW+WLIK+VIRVYL SEQRLCNNIFEG GSYS
Sbjct: 241 YLLILEMERHSIGDLQTMEWDVLNVKIKKWIWLIKVVIRVYLASEQRLCNNIFEGFGSYS 300

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKG 360
           A CF EISATSML LLNFGEAIAM   RPEKLF  LDMYEVL+NLL  +  LFSE+TE G
Sbjct: 301 ATCFAEISATSMLCLLNFGEAIAMTTRRPEKLFRFLDMYEVLENLLGDIGALFSEDTEMG 360

Query: 361 SFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
           SFLK +FHNLF KLG SARATF+DFG+ IA SIST+PFPGGGVHHLT+YVMNYI TLTV+
Sbjct: 361 SFLKADFHNLFMKLGDSARATFVDFGSLIARSISTSPFPGGGVHHLTKYVMNYIRTLTVY 420

Query: 421 RDSLVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDAL 480
           RDSLVFLL+D+A D L P +EL +E+NS+PCP+A+HLQSITS L+SNLNNKSKLY DDAL
Sbjct: 421 RDSLVFLLRDEAADGLGPASELLNEINSMPCPLAFHLQSITSQLISNLNNKSKLYNDDAL 480

Query: 481 RHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLDG 540
           RHIFLMNNIHY+VQKV+ S+L AF G  W+REH RM Q HA IY R TWQSV+SLLRLDG
Sbjct: 481 RHIFLMNNIHYMVQKVKGSELRAFFGGEWIREHNRMLQIHANIYTRVTWQSVISLLRLDG 540

Query: 541 DGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCGS 600
           DG  T KAVFKEKYRAFN AFEEIYK QTGWNV DP LRDDL I TSNCVIQAYR L   
Sbjct: 541 DGTNTPKAVFKEKYRAFNVAFEEIYKTQTGWNVSDPDLRDDLHILTSNCVIQAYRFLRNL 600

Query: 601 RSQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635
           +SQFN EKYIKYT DDLSKH+LDL QGS R LQSSRRR
Sbjct: 601 KSQFNCEKYIKYTIDDLSKHILDLFQGSPRLLQSSRRR 638

BLAST of Cucsat.G14657 vs. ExPASy TrEMBL
Match: A0A6J1C7N5 (Exocyst subunit Exo70 family protein OS=Momordica charantia OX=3673 GN=LOC111008738 PE=3 SV=1)

HSP 1 Score: 976 bits (2524), Expect = 0.0
Identity = 490/639 (76.68%), Postives = 549/639 (85.92%), Query Frame = 0

Query: 1   MGDCESIIPTSEGEQHALAAFQHLVKFLRTSQNVNNELKKLLADLDSHLTSLTSYT---V 60
           MGDCESI+P SEGE H +AAFQHLV+ L  SQNVN+E+K+LL DLDSHLTS+T YT   +
Sbjct: 12  MGDCESILPASEGEHHVIAAFQHLVRVLGASQNVNDEVKRLLNDLDSHLTSMTQYTHLEI 71

Query: 61  GKLSELETRFKSAKEKITRWESNKSMIWDSGPKEASEYMKAVDEIHKVQEGLRSLSANDS 120
           GKLSELE +FKSA+EKI RWESNKS+IWDSGP EA EYMKAV EIHKVQEGLRSL  NDS
Sbjct: 72  GKLSELEKKFKSAEEKIRRWESNKSIIWDSGPMEACEYMKAVGEIHKVQEGLRSLPVNDS 131

Query: 121 QKQNELLFQADSVVQIAMARLEQELIHILAQHKLYFEPDYVPFQSGGYDVVYNESFVSAE 180
           +KQ ELLFQADSV+QIAMARLEQEL+HILAQHKLYFEPDY+PFQSGGYD VYNESF SAE
Sbjct: 132 RKQKELLFQADSVMQIAMARLEQELVHILAQHKLYFEPDYIPFQSGGYDFVYNESFASAE 191

Query: 181 DSLEVETSHGESIPALYAVNLVHPHVIAHLKSIANVMFNSNYIQEFCLAFVRMRKDALNE 240
           D+L  ETS GES+ A+Y VNLVHPHVI HLKSIANVMF SNYIQEFC AFV  R+DAL+E
Sbjct: 192 DNLGEETSQGESVAAVYVVNLVHPHVIPHLKSIANVMFTSNYIQEFCKAFVNTRRDALDE 251

Query: 241 YLFILEMEKFSIGDLLTMEWSDLNVKIKKWVWLIKIVIRVYLTSEQRLCNNIFEGIGSYS 300
           YLF+L+MEK+SIGDLLTM W  LNVKIK+WV +I+I+IRVYL SE+RLCNNIFEGIGSYS
Sbjct: 252 YLFVLQMEKYSIGDLLTMNWDILNVKIKRWVCVIQIIIRVYLASERRLCNNIFEGIGSYS 311

Query: 301 AVCFTEISATSMLRLLNFGEAIAMEPHRPEKLFHLLDMYEVLKNLLEFVDELFSEETEKG 360
           + CFT+ISATSM RLLNFGEAIAM P RPEKLF LLDMYEVL+NLL   D LF E++E G
Sbjct: 312 SACFTDISATSMFRLLNFGEAIAMTPRRPEKLFRLLDMYEVLENLLVDKDALFPEDSETG 371

Query: 361 SFLKFEFHNLFKKLGVSARATFLDFGNFIACSISTNPFPGGGVHHLTRYVMNYIHTLTVF 420
           SFLK EFHNLFKKLG SARATF+DFG  I+ SIST+PFPGGGVHHLT+YVMNYI TLTV+
Sbjct: 372 SFLKSEFHNLFKKLGDSARATFVDFGYLISSSISTDPFPGGGVHHLTKYVMNYIKTLTVY 431

Query: 421 RDSLVFLLQDQATDVLSPTTELQSEVNSIPCPMAYHLQSITSHLLSNLNNKSKLYKDDA- 480
           RDSL+FLLQD+  D LSP +EL +E N++ CPMAYHLQSI SHL+SNLNNKSKLY+DDA 
Sbjct: 432 RDSLIFLLQDKVADNLSPDSELHNE-NNVHCPMAYHLQSIASHLISNLNNKSKLYRDDAA 491

Query: 481 LRHIFLMNNIHYIVQKVENSDLIAFLGSGWMREHIRMFQSHATIYMRATWQSVLSLLRLD 540
           LRH+FLMNNIHY+VQKV   +L  + G+ WMREH + FQ+HAT+Y+R TWQSVLS+L+ D
Sbjct: 492 LRHVFLMNNIHYVVQKVGGPELRLYFGNKWMREHTKTFQNHATLYIRVTWQSVLSVLKHD 551

Query: 541 GDGMKTSKAVFKEKYRAFNAAFEEIYKNQTGWNVPDPQLRDDLLIQTSNCVIQAYRILCG 600
           G G   SKA FKEKYRAF  AFEEIYKNQTGWNVPDP LRDDL IQTSNCVIQAYR LC 
Sbjct: 552 GKGTNISKADFKEKYRAFTVAFEEIYKNQTGWNVPDPDLRDDLHIQTSNCVIQAYRTLCE 611

Query: 601 SRSQFNREKYIKYTTDDLSKHMLDLLQGSSRSLQSSRRR 635
           +RS FNREKYIKYT DDL KH+LDL Q S RSLQSSRRR
Sbjct: 612 TRSPFNREKYIKYTPDDLGKHLLDLFQDSPRSLQSSRRR 649

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FNR34.8e-14144.03Exocyst complex component EXO70E2 OS=Arabidopsis thaliana OX=3702 GN=EXO70E2 PE=... [more]
Q9LZD31.1e-6529.80Exocyst complex component EXO70A1 OS=Arabidopsis thaliana OX=3702 GN=EXO70A1 PE=... [more]
Q9FGH92.5e-6528.35Exocyst complex component EXO70B1 OS=Arabidopsis thaliana OX=3702 GN=EXO70B1 PE=... [more]
Q9LMJ41.5e-5428.37Exocyst complex component EXO70B2 OS=Arabidopsis thaliana OX=3702 GN=EXO70B2 PE=... [more]
Q8VY274.1e-5227.18Exocyst complex component EXO70H1 OS=Arabidopsis thaliana OX=3702 GN=EXO70H1 PE=... [more]
Match NameE-valueIdentityDescription
XP_004137600.10.099.84exocyst complex component EXO70E2 [Cucumis sativus] >XP_031744501.1 exocyst comp... [more]
XP_008450312.10.093.10PREDICTED: exocyst complex component EXO70A1 [Cucumis melo] >XP_008450459.1 PRED... [more]
TYJ98937.10.093.03exocyst complex component EXO70A1 [Cucumis melo var. makuwa][more]
XP_038893966.10.088.40exocyst complex component EXO70E2 [Benincasa hispida][more]
XP_023520029.10.079.15exocyst complex component EXO70E2 [Cucurbita pepo subsp. pepo] >XP_023520030.1 e... [more]
Match NameE-valueIdentityDescription
A0A1S4DU880.093.10Exocyst subunit Exo70 family protein OS=Cucumis melo OX=3656 GN=LOC103491934 PE=... [more]
A0A5D3BIJ10.093.03Exocyst subunit Exo70 family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1KLM60.078.21Exocyst subunit Exo70 family protein OS=Cucurbita maxima OX=3661 GN=LOC111495300... [more]
A0A6J1E6T20.078.21Exocyst subunit Exo70 family protein OS=Cucurbita moschata OX=3662 GN=LOC1114313... [more]
A0A6J1C7N50.076.68Exocyst subunit Exo70 family protein OS=Momordica charantia OX=3673 GN=LOC111008... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 60..80
NoneNo IPR availableGENE3D1.20.1280.170Exocyst complex component Exo70coord: 73..632
e-value: 1.8E-145
score: 487.6
NoneNo IPR availablePANTHERPTHR12542:SF92EXOCYST COMPLEX COMPONENT EXO70E2coord: 23..629
IPR004140Exocyst complex component Exo70PFAMPF03081Exo70coord: 265..619
e-value: 1.2E-103
score: 347.0
IPR004140Exocyst complex component Exo70PANTHERPTHR12542EXOCYST COMPLEX PROTEIN EXO70coord: 23..629
IPR016159Cullin repeat-like-containing domain superfamilySUPERFAMILY74788Cullin repeat-likecoord: 83..621

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G14657.T1Cucsat.G14657.T1mRNA
Cucsat.G14657.T2Cucsat.G14657.T2mRNA
Cucsat.G14657.T3Cucsat.G14657.T3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006887 exocytosis
biological_process GO:0015031 protein transport
cellular_component GO:0000145 exocyst