Cucsat.G14086 (gene) Cucumber (B10) v3

Overview
NameCucsat.G14086
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionL-ascorbate oxidase-like protein
Locationctg1869: 2480621 .. 2483797 (-)
RNA-Seq ExpressionCucsat.G14086
SyntenyCucsat.G14086
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGGGAAACAGATAACAACCCATCTTGTTCAATTATTGGGGAGATTTTGAGGGTAAAAACCTGCTCGAGGAAAAAGAGCAGTGACTTAGCAAAAAGGATCATAGAGAAGGGGGAGGGATACATATGGCTAGGTTGACGTTGTCCTTGATGCTATGCCTGCTCTCAGTTGCAGCAATGCAAACCGTTAGGGGTGAAGATCCTTATTTCTTCTTCACGTGGAATGTCACTTATGGCACCATCTCTCCCTTGGGTGTTCCACAGCAAGGCATTCTCATCAACGGTCAATTCCCCGGACCTAATATCAACTCCACGACAAACAATAACCTAGTCATCAATGTTTTCAATAATCTCGATGAACCCTTCCTTCTACATTGGTATAATAATTATATTAATTAAGTTTACATCTACATGCATGTAATTATAAATATATTTATTATTTGTTAATGGCTTATTGGGATTGCAGGAGTGGTATTCAACATAGAAAGAACTCGTGGCAGGATGGACTACTTGGAACCAATTGCCCAATCCCACCAGGCACAAACTTTACTTATCATTTCCAAGTTAAGGATCAAATCGGAAGCTTCTTCTACTACCCATCAACTGCCATGCACAAAGCTGTAGGAGCCTTTGGCGGCCTTCGAGTGAACAGTCGTTTACTCATTCCCGTTCCTTATGCTGATCCCGAAGATGATTACACTGTGCTTATTGGTGACTGGTACACCAAAAGCCATTCCACTCTTAAGAAGTTCTTGGACAGTGGTCGCTCCATCGCTAGACCTGATGGGGTCCTAATTAACGGAAGGATTGCGAAGGGTGATGGAACTGATGAGCCACTCTTCACCATGAAACCTGAAAAGACTTACAAGTACCGAATATGCAACGTTGGACTAAAGACATCTCTTAACTTTAGGATCCAAGGCCACACCATGAAGTTGGTGGAAATGGAAGGCTCCCATACTGTCCAAAATGTTTACCAATCACTTGATGTCCATGTGGGACAATGTTTTTCCGTGCTAGTCACAGCCGACAAAGACCCTAAAGACTATTACATGGTTGCATCTACTCGATTCACTAAGAACATTCTTGTCGGTAAAGGAATTATTCGATACACCAATGGAAAGGCCCCTGCTTCTCCTGAAATTCCTGAGGCACCTAGGGGTTGGGCTTGGTCTCTCAATCAATTTCGTACCTTTCGTTGGAACCTCACTGCCAGTGCTGCTAGGCCCAATCCTCAAGGCTCGTATCACTATGGTTCTATCAACATTACTCGCACCATCAAGATAGTCAATTCTGCCACTAACGTTGATGGTAAGTTGAGATATGCCATCAATGGAGTCTCTCATGTCGATCCTGAAACTCCATTGAAGCTTGCAGAATATTTCGAAGTTGCTGATAAGGTCTTCAAGTATGATACAATTTCTGATGAAGGATTGTCAAAAACCGCAAACATTGTGACTGTTGCTCCTAATGTTTTGAATGCAACTTTTCGCAACTTTGTAGAGATTGTATTTGAGAACCAGGAAAAGAGCCTCCAGTCATGGCACTTAGATGGCTACTCCTTCTTTGCTGTTGCGTAAGTATAAATGTCCATAAATAAATGTACATATGTACATTATATACATTCCACACATGACTAATGAATTAATACATTCTACACACAACTAATGAATTATTTATCCAATATTTCATGCAGCGTTGAGCCTGGGAGATGGTCTCCGGAGAAGCGAAAGAATTACAATCTTCTAGACGCTGTGAGCAGACATACAATCCAAGTTTTTCCTAAATCATGGGCAGCTATTCTTCTTACGTTAGACAATGCTGGAATGTGGAACTTGAGGTCTGAGCTGACAGAAAATCGTTACTTGGGACAACAGATGTACTTTAGTGTTCAATCACCTGCTCGCTCCCTCAGAGACGAATACAACATCCCCGATAACACTTTGCTTTGCGGTTTGGTCAAAGACATGCCATTGCCTCCTCCATATACCATCTGAGACACTATTATTGATAGACACCCCTCCCCATTTCATTTAATTGAGAGACTATGATATTTATAAGGTGGATCATGAATCTCTCAAAATGTTGCTATGATTTAAATTCATGAAAGCCCTCAAAAGATCCCTAAATAGGAAGTATGTTACTCCTATATTGCAATCAAGGAAAACCTAATAACAAATCAGACTTTTTTTTCCCTCCAAAGGGACCACTAGGCCAAGCCGACTCATTACACCAAATTGCTTTGTGGCATATCAACATCATTTTATCCATAATTTAAAAGGAGAAATTTTAATAAATAGATCGCACAATATTTTTTATATTATAATTTATAATACTCAAACTTCTCTACTTCCTTCGCTCAAATTTATAATACTCAAACTTCTCTACTTCCTTCGCTGAGTTTATTTTATTGAGCTGCAAGTTATATGTAAGTTAATTAACTGCCTCGTCTTTGGTTAATTAACTGCCTCGTATTTACATGTTCTTAACTATTAAAATACGAAACTCGATTTACAATTATCAGGATTTATAGTGAATTATGGCCAGAAATTAAATATTCATTTATTGTATTGTAATATATATAAAGTTTAGTACAAATCAGTAAAATTTGAAAAAATATTTATAATATTTTCAACATAATTTACCTATTTTTCAAAAGTTGATTTTTTAACAAATTAAAAAAAAAAAACATGTTTTGGAGGAAGTTATTTTAAAAAATAACAAATATTGAAAGTATATAATGAAATTCATATACGGTTTAAAATCCTCAATTTTGCAAAATTCATTTTTCCCATTTTAGTTTCTCTATCTGTCGTCCGTCGAACCCAAATTCATCCCTAATGCCCTTTTCCTCTTTGACTATATATCCGGGAATTTCCCCGCCTCCATGAAGTCCTACACTCAGCCACGAGCAAGAATGATGCCACCCAATGGTATTTGCAGTCCCTGCAATGCGAACCTTAACCCAGCACCTAAATTTCGACTACTTTCTCTATCTGCAATTCCAAACGTGTTACATTTTCCAAATCCACCTTCTCTCTACCTCGGTTCCAGCTCAAACTATTCCGGATTTTCCCGCTTGGTTGGTCCACCAAGGAAAATGGATTCTCAAACCAGAAGTCGCTTAACATTTGTAAATTATGGTGCAGGAGCTACAAGGGCCGCCGCTACTGATCACT

Coding sequence (CDS)

ATGGCTAGGTTGACGTTGTCCTTGATGCTATGCCTGCTCTCAGTTGCAGCAATGCAAACCGTTAGGGGTGAAGATCCTTATTTCTTCTTCACGTGGAATGTCACTTATGGCACCATCTCTCCCTTGGGTGTTCCACAGCAAGGCATTCTCATCAACGGTCAATTCCCCGGACCTAATATCAACTCCACGACAAACAATAACCTAGTCATCAATGTTTTCAATAATCTCGATGAACCCTTCCTTCTACATTGGAGTGGTATTCAACATAGAAAGAACTCGTGGCAGGATGGACTACTTGGAACCAATTGCCCAATCCCACCAGGCACAAACTTTACTTATCATTTCCAAGTTAAGGATCAAATCGGAAGCTTCTTCTACTACCCATCAACTGCCATGCACAAAGCTGTAGGAGCCTTTGGCGGCCTTCGAGTGAACAGTCGTTTACTCATTCCCGTTCCTTATGCTGATCCCGAAGATGATTACACTGTGCTTATTGGTGACTGGTACACCAAAAGCCATTCCACTCTTAAGAAGTTCTTGGACAGTGGTCGCTCCATCGCTAGACCTGATGGGGTCCTAATTAACGGAAGGATTGCGAAGGGTGATGGAACTGATGAGCCACTCTTCACCATGAAACCTGAAAAGACTTACAAGTACCGAATATGCAACGTTGGACTAAAGACATCTCTTAACTTTAGGATCCAAGGCCACACCATGAAGTTGGTGGAAATGGAAGGCTCCCATACTGTCCAAAATGTTTACCAATCACTTGATGTCCATGTGGGACAATGTTTTTCCGTGCTAGTCACAGCCGACAAAGACCCTAAAGACTATTACATGGTTGCATCTACTCGATTCACTAAGAACATTCTTGTCGGTAAAGGAATTATTCGATACACCAATGGAAAGGCCCCTGCTTCTCCTGAAATTCCTGAGGCACCTAGGGGTTGGGCTTGGTCTCTCAATCAATTTCGTACCTTTCGTTGGAACCTCACTGCCAGTGCTGCTAGGCCCAATCCTCAAGGCTCGTATCACTATGGTTCTATCAACATTACTCGCACCATCAAGATAGTCAATTCTGCCACTAACGTTGATGGTAAGTTGAGATATGCCATCAATGGAGTCTCTCATGTCGATCCTGAAACTCCATTGAAGCTTGCAGAATATTTCGAAGTTGCTGATAAGGTCTTCAAGTATGATACAATTTCTGATGAAGGATTGTCAAAAACCGCAAACATTGTGACTGTTGCTCCTAATGTTTTGAATGCAACTTTTCGCAACTTTGTAGAGATTGTATTTGAGAACCAGGAAAAGAGCCTCCAGTCATGGCACTTAGATGGCTACTCCTTCTTTGCTGTTGCCGTTGAGCCTGGGAGATGGTCTCCGGAGAAGCGAAAGAATTACAATCTTCTAGACGCTGTGAGCAGACATACAATCCAAGTTTTTCCTAAATCATGGGCAGCTATTCTTCTTACGTTAGACAATGCTGGAATGTGGAACTTGAGGTCTGAGCTGACAGAAAATCGTTACTTGGGACAACAGATGTACTTTAGTGTTCAATCACCTGCTCGCTCCCTCAGAGACGAATACAACATCCCCGATAACACTTTGCTTTGCGGTTTGGTCAAAGACATGCCATTGCCTCCTCCATATACCATCTGA

Protein sequence

MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYTNGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNVLNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQVFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCGLVKDMPLPPPYTI
Homology
BLAST of Cucsat.G14086 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 877.9 bits (2267), Expect = 6.2e-254
Identity = 401/542 (73.99%), Postives = 459/542 (84.69%), Query Frame = 0

Query: 8   LMLCL-LSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTNN 67
           L  CL L+ AA   V  EDPYF   WNVTYGT SPLGVPQQ ILINGQFPGPNINST+NN
Sbjct: 7   LAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNN 66

Query: 68  NLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFY 127
           N++INVFNNLDEPFLL W+GIQHRKN WQDG  GT CPI PGTN+TYHFQ KDQIGS+FY
Sbjct: 67  NVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFY 126

Query: 128 YPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSI 187
           YP+T MH+A G +GGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSH+ LKKFLD GR+I
Sbjct: 127 YPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTI 186

Query: 188 ARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEG 247
            RPDG++ING+  KGDG+D PLFT+KP KTY+ RICNVG+KTS+NFRIQ H MKLVEMEG
Sbjct: 187 GRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEG 246

Query: 248 SHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYTNGKAPA 307
           SH +QN Y SLDVHVGQCF  +VTA+++PKDYYMVAS+RF K ++   G++RY  GK PA
Sbjct: 247 SHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGPA 306

Query: 308 SPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSATNVDG 367
           S ++P  P GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK+VN+   VDG
Sbjct: 307 SSQLPAGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDG 366

Query: 368 KLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNVLNATFR 427
           KLR+A+NGVSH +PETPLKLAEYF ++DKVFKYDTI+D+   +    + + PNVLN T R
Sbjct: 367 KLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHR 426

Query: 428 NFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQVFPKSW 487
            FVE+VFEN EKS+QSWHLDGYSFF+VAVEPG W+PEKRKNYNLLDAVSRHT+QV+PK W
Sbjct: 427 TFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCW 486

Query: 488 AAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCGLVKDMP 547
           AAILLT DN GMWN+RSE TE RYLGQQ+Y SV SP +SLRDEYN+P+ +L CGLVK+ P
Sbjct: 487 AAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKNTP 546

Query: 548 LP 549
            P
Sbjct: 547 KP 548

BLAST of Cucsat.G14086 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 5.4e-234
Identity = 373/553 (67.45%), Postives = 445/553 (80.47%), Query Frame = 0

Query: 3   RLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 62
           ++T   +L  LSV     V  EDPY +F WNVTYGTI+PLGVPQQGILINGQFPGP IN 
Sbjct: 5   KVTFVALLLCLSVG----VIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINC 64

Query: 63  TTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIG 122
           T+NNN+V+NVFNNLDEPFL  W+G+QHRKNSWQDG  GT CPI PG NFTY FQVKDQIG
Sbjct: 65  TSNNNIVVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIG 124

Query: 123 SFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDS 182
           S+ Y+P+TA+H+A G +G L V+SR LIPVP+ +P D+Y V +GDWY K H TLKK LD 
Sbjct: 125 SYSYFPTTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDG 184

Query: 183 GRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMKLV 242
           GR+I RPDG++ING+ AK     EPLFTM+  KTY+YR CN+G+++S+N R QGH MKLV
Sbjct: 185 GRTIGRPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLV 244

Query: 243 EMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYTNG 302
           E+EGSHTVQN+Y SLD+HVGQC SVLVTAD++PKDYY+V S+RF K  L    IIRY NG
Sbjct: 245 ELEGSHTVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANG 304

Query: 303 KAPASPEIPEAP----RGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIV 362
           K PASPE+P  P     G AWS+NQFR+FRWNLTASAARPNPQGSYHYG INITRTIKI 
Sbjct: 305 KGPASPELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIF 364

Query: 363 NSATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAP 422
           NS + V GKLRY +NG+SH + ETPLKL EYF   +K FKYD ++DE  +  + + T+A 
Sbjct: 365 NSMSQVGGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKL-TIAT 424

Query: 423 NVLNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHT 482
           NV NAT+RNFVEI+FEN EK+++++HLDGYSFFAVAVEPGRWSPEKRKNYNL+D +SR+ 
Sbjct: 425 NVKNATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNN 484

Query: 483 IQVFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLL 542
           IQV+P SWAAI+LT DNAGMWNLRSE+ E  YLG+Q+YFSV SP+RSLRDEYNIPDN  L
Sbjct: 485 IQVYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPL 544

Query: 543 CGLVKDMPLPPPY 552
           CG+VK + +P PY
Sbjct: 545 CGIVKGLSMPAPY 552

BLAST of Cucsat.G14086 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.5e-156
Identity = 268/540 (49.63%), Postives = 358/540 (66.30%), Query Frame = 0

Query: 25  DPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTNNNLVINVFNNLDEPFLLHW 84
           DPY F+ + V+Y T SPLGVPQQ I ING+FPGP IN TTN NLV+NV N LDE  LLHW
Sbjct: 22  DPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENLVVNVRNKLDEGLLLHW 81

Query: 85  SGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFYYPSTAMHKAVGAFGGLRV 144
           +GIQ R+ SWQDG+LGTNCPIPP  N+TY FQVKDQIGSFFY+PS    +A G FG   V
Sbjct: 82  NGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFPSLHFQRASGGFGSFVV 141

Query: 145 NSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSIARPDGVLINGR------- 204
           N R +IPVP++ P+ D TV IGDWY ++H+ L+K LD G+ +  PDGVLING+       
Sbjct: 142 NPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDLGMPDGVLINGKGPYRYND 201

Query: 205 IAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEGSHTVQNVYQSL 264
               DG D    T+ P KTY+ R+ NVG+ TSLNFRIQGH + L E EGS+TVQ  Y SL
Sbjct: 202 TLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVLAESEGSYTVQQNYTSL 261

Query: 265 DVHVGQCFSVLVTADKD-PKDYYMVASTRFTKNIL----VGKGIIRYTNGKAPASPEIPE 324
           D+HVGQ +S LVT D++   DYY+VAS R     +     G GI++YTN K  A  ++P 
Sbjct: 262 DIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGILKYTNSKGKAKGQLPP 321

Query: 325 APR---GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVN-SATNVDGKL 384
            P+      +S+NQ R+ RWN++AS ARPNPQGS+ YGSIN+T    + N     + GK 
Sbjct: 322 GPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTDVYVLRNMPPVTISGKR 381

Query: 385 RYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNVLNATFRNF 444
           R  +NG+S  +P TP++LA+  +V D V+K D      L+  A + T   +++N T+R F
Sbjct: 382 RTTLNGISFKNPSTPIRLADKLKVKD-VYKLD-FPKRPLTGPAKVAT---SIINGTYRGF 441

Query: 445 VEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQVFPKSWAA 504
           +E+V +N +  +QS+H+ GY+FF V ++ G W+   R  YN  D ++R TIQV+P +W+A
Sbjct: 442 MEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIARSTIQVYPGAWSA 501

Query: 505 ILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCGLVKDMPLP 549
           IL++LDN G WNLR+E  ++ YLGQ+ Y  V +P  + + E+  PDN L CG +  +  P
Sbjct: 502 ILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPDNVLYCGALSKLQKP 556

BLAST of Cucsat.G14086 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 2.4e-149
Identity = 261/558 (46.77%), Postives = 370/558 (66.31%), Query Frame = 0

Query: 4   LTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINST 63
           L  S +LC   ++A+      DP+  + + V+Y T SPLGVPQQ I +NGQFPGP +N+T
Sbjct: 7   LLASFLLCFALLSAVSF--AADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNAT 66

Query: 64  TNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGS 123
           TN N+V+NVFN+LDEP LL W GIQ R+NSWQDG+LGTNCPIPP  NFTY FQVKDQIGS
Sbjct: 67  TNYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGS 126

Query: 124 FFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSG 183
           FFY PS    +A G FG + +N+R +IP+P+  P+ +   +IGDWYT+ H  L++ LDSG
Sbjct: 127 FFYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSG 186

Query: 184 RSIARPDGVLINGR------IAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGH 243
           + +  PDGVLING+       +  DG D   F ++P KTY+ R+ NVG+ TSLNFRIQ H
Sbjct: 187 KELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNH 246

Query: 244 TMKLVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKD-PKDYYMVASTRFTKNI----LV 303
           ++ LVE EG +T Q  +   DVHVGQ +S LVT D+D   DYY+VAS RF        + 
Sbjct: 247 SLLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVT 306

Query: 304 GKGIIRYTNGKAPASPEIP----EAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGS 363
           G  I+ Y+N K P S  +P    +    W+ +++Q +T R N +AS ARPNPQGS+HYG 
Sbjct: 307 GVAILHYSNSKGPVSGPLPVPKTDVSSPWS-AMSQPKTIRQNTSASGARPNPQGSFHYGQ 366

Query: 364 INITRTIKIVN-SATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGL 423
           INIT T  + +   T ++G LR  +NG+S V+P TP++LA+  +V    +K D   D   
Sbjct: 367 INITNTYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKG-AYKLD-FPDRPF 426

Query: 424 SKTANIVTVAPNVLNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKN 483
           ++    + +  +++NAT++ F+++VF+N +  +QS+H+DGYSFF V ++ G WS +K+ +
Sbjct: 427 NRP---LRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGS 486

Query: 484 YNLLDAVSRHTIQVFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLR 543
           YN  DA+SR TI+V+P  W A+L++LDN G+WN+R E  +  YLG++ Y  + +P    +
Sbjct: 487 YNNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGK 546

Query: 544 DEYNIPDNTLLCGLVKDM 546
            E + PDN L CG +K++
Sbjct: 547 TEMDPPDNVLYCGALKNL 556

BLAST of Cucsat.G14086 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 4.6e-148
Identity = 249/539 (46.20%), Postives = 364/539 (67.53%), Query Frame = 0

Query: 25  DPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTNNNLVINVFNNLDEPFLLHW 84
           DPY  + + ++Y T SPLGVPQQ I +NG+FPGP IN+TTN N+ +NV N+LDEP LL W
Sbjct: 25  DPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLLTW 84

Query: 85  SGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFYYPSTAMHKAVGAFGGLRV 144
            G+Q R+NSWQDG+LGTNCPIPP  NFTY FQ+KDQIGS+FY PS    +A G FG L +
Sbjct: 85  PGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGALII 144

Query: 145 NSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSIARPDGVLINGR------I 204
           N+R L+P+P+ +P+ +   +IGDWYT++H+ L++ LDSG+ +  PDGVLING+       
Sbjct: 145 NNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKYNS 204

Query: 205 AKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEGSHTVQNVYQSLD 264
           +  DG +     + P KTY+ R+ NVG+ TSLNFRIQ H + L+E EG +T Q  +   D
Sbjct: 205 SVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTDFD 264

Query: 265 VHVGQCFSVLVTADKD-PKDYYMVASTRFTKNI----LVGKGIIRYTNGKAPASPEIP-- 324
           VHVGQ +S LVT D++   DYY+VAS RF        + G GI+ Y+N K PAS  +P  
Sbjct: 265 VHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLPVS 324

Query: 325 --EAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVN-SATNVDGKL 384
             +    W+ ++NQ R  + N +AS ARPNPQGS+HYG INITRT  + +   T ++GKL
Sbjct: 325 ATDVNHPWS-AMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKINGKL 384

Query: 385 RYAINGVSHVDPETPLKLAEYFEV-ADKVFKY-DTISDEGLSKTANIVTVAPNVLNATFR 444
           R  +NG+S V+P TP++LA+  +V  D +  + D   DE L +      ++ +++NAT++
Sbjct: 385 RATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPR------LSSSIINATYK 444

Query: 445 NFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQVFPKSW 504
            F++++F+N +  +QS+H+DGY+F+ VA++ G WS ++  +YN  DAV+R T++V+P +W
Sbjct: 445 GFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAW 504

Query: 505 AAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCGLVKDM 546
            A+L++LDN G+WN+R E  +  YLGQ+ Y  + +P  +   E + P+N + CG ++ M
Sbjct: 505 TAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAM 556

BLAST of Cucsat.G14086 vs. NCBI nr
Match: XP_004137383.1 (L-ascorbate oxidase homolog [Cucumis sativus] >KAE8652494.1 hypothetical protein Csa_013569 [Cucumis sativus])

HSP 1 Score: 1139 bits (2947), Expect = 0.0
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL
Sbjct: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300

Query: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420
           ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV
Sbjct: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420

Query: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480
           LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ
Sbjct: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPPPYTI 553
           LVKDMPLPPPYTI
Sbjct: 541 LVKDMPLPPPYTI 553

BLAST of Cucsat.G14086 vs. NCBI nr
Match: KAA0036016.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 522/553 (94.39%), Postives = 539/553 (97.47%), Query Frame = 0

Query: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLT +L+LCLL VAAM TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMH+A GAFGGLRVNSRLLIPVPY DPEDDYTVLIGDWYTKS+STLKKFL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSYSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLING+IAKGDGTDEPLFTM P KTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTAD+DP+DYYMVAS+RFTKNILVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420
           ATNVDGKLRYAINGVSH+DPETPLKLAEYFEVADKVFKYDTISDEG +KTAN VT+APNV
Sbjct: 361 ATNVDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480
           +NATFRNFVEI+FEN EKSLQSWHLDGYSFFAVA+EPGRWSPEKRKNYNLLDAVSRHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540
           VFPKSW AILLTLDNAGMWNLRSELTENRYLGQQ+YFSVQSPARSLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPPPYTI 553
           LVKDM LP PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of Cucsat.G14086 vs. NCBI nr
Match: XP_008438536.1 (PREDICTED: L-ascorbate oxidase homolog [Cucumis melo] >TYJ98816.1 L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1090 bits (2820), Expect = 0.0
Identity = 521/553 (94.21%), Postives = 538/553 (97.29%), Query Frame = 0

Query: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLT +L+LCLL VAAM TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMH+A GAFGGLRVNSRLLIPVPY DPEDDYTVLIGDWYTKSHSTLKKFL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSHSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLING+IAKGDGTDEPLFTM P KTYKYRICNVGLK SLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKISLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTAD+DP+DYYMVAS+RFTKNILVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420
           ATN+DGKLRYAINGVSH+DPETPLKLAEYFEVADKVFKYDTISDEG +KTAN VT+APNV
Sbjct: 361 ATNIDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480
           +NATFRNFVEI+FEN EKSLQSWHLDGYSFFAVA+EPGRWSPEKRKNYNLLDAVSRHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540
           VFPKSW AILLTLDNAGMWNLRSELTENRYLGQQ+YFSVQSPARSLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPPPYTI 553
           LVKDM LP PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of Cucsat.G14086 vs. NCBI nr
Match: XP_022153749.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1044 bits (2699), Expect = 0.0
Identity = 493/553 (89.15%), Postives = 526/553 (95.12%), Query Frame = 0

Query: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MA L  +++LCL S AAM TVRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLMFTVLLCL-SAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NST+NNN+V+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMH+A G FGGLRVNSRLLIPVPYADPEDDYTVL+GDWYTKSHS LK FL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFL 180

Query: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRS+ARPDGVLING+ AKGDGTDEPLFTMKPEKTYKYR+CN+GLKTSLN R Q HTMK
Sbjct: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300
           LVEMEGSH VQN YQSLD+HVGQCFSVLVTA+++PKDYY++ASTRFTKN+LVGKGIIRYT
Sbjct: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300

Query: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           NGK PASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKI+NS
Sbjct: 301 NGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360

Query: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420
           AT VDGKLRYAINGVSH+DPETPLKLAEYF +ADKVFKYDTISD+G ++ AN VTVAPNV
Sbjct: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNV 420

Query: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480
           +NATFRNFVEIVFEN EKSLQSWHL+GYSFFAVA+EPGRW+PEKRKNYNLLDAVSR TIQ
Sbjct: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQ+YFSV SPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPPPYTI 553
           LVKDMPLP PYTI
Sbjct: 541 LVKDMPLPKPYTI 552

BLAST of Cucsat.G14086 vs. NCBI nr
Match: XP_011654310.2 (L-ascorbate oxidase homolog [Cucumis sativus] >KGN54106.2 hypothetical protein Csa_021661 [Cucumis sativus])

HSP 1 Score: 1041 bits (2692), Expect = 0.0
Identity = 491/553 (88.79%), Postives = 519/553 (93.85%), Query Frame = 0

Query: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MA +  +++LCL +   M TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPN+
Sbjct: 1   MAGMMFTMLLCLSAAGMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNV 60

Query: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMH+A G FGG+RVNSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK+FL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGIRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQFL 180

Query: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLING+ +K DG DEPLFTMKP KTYKYR+CNVGLKTSLNFR QGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTSKDDGIDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300
           LVEMEGSHTVQN Y+SLDVHVGQCFSVLVTAD++PKDYYMVASTRF K+ LVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYKSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKSALVGKGIVRYT 300

Query: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           NGK PASPEIP AP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 NGKGPASPEIPNAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420
           AT VDGKLRYAINGVSHVDPETPLKLAEYFE+ DKVFKYDTISDEGL++    VTVAPNV
Sbjct: 361 ATKVDGKLRYAINGVSHVDPETPLKLAEYFEITDKVFKYDTISDEGLAEGVTTVTVAPNV 420

Query: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480
           +N TFRNF+EI+FEN EKSLQSWHLDGYSFFAVA+EPGRWSPEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNTTFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DNAGMWNLRSELTENRYLGQQ+Y SVQSPARSLRDEYNIPD TLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGQQLYMSVQSPARSLRDEYNIPDKTLLCG 540

Query: 541 LVKDMPLPPPYTI 553
           LVKD+PLP PYTI
Sbjct: 541 LVKDLPLPKPYTI 553

BLAST of Cucsat.G14086 vs. ExPASy TrEMBL
Match: A0A5A7T1B7 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00010 PE=3 SV=1)

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 522/553 (94.39%), Postives = 539/553 (97.47%), Query Frame = 0

Query: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLT +L+LCLL VAAM TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMH+A GAFGGLRVNSRLLIPVPY DPEDDYTVLIGDWYTKS+STLKKFL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSYSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLING+IAKGDGTDEPLFTM P KTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTAD+DP+DYYMVAS+RFTKNILVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420
           ATNVDGKLRYAINGVSH+DPETPLKLAEYFEVADKVFKYDTISDEG +KTAN VT+APNV
Sbjct: 361 ATNVDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480
           +NATFRNFVEI+FEN EKSLQSWHLDGYSFFAVA+EPGRWSPEKRKNYNLLDAVSRHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540
           VFPKSW AILLTLDNAGMWNLRSELTENRYLGQQ+YFSVQSPARSLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPPPYTI 553
           LVKDM LP PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of Cucsat.G14086 vs. ExPASy TrEMBL
Match: A0A5D3BIA5 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00010 PE=3 SV=1)

HSP 1 Score: 1090 bits (2820), Expect = 0.0
Identity = 521/553 (94.21%), Postives = 538/553 (97.29%), Query Frame = 0

Query: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLT +L+LCLL VAAM TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMH+A GAFGGLRVNSRLLIPVPY DPEDDYTVLIGDWYTKSHSTLKKFL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSHSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLING+IAKGDGTDEPLFTM P KTYKYRICNVGLK SLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKISLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTAD+DP+DYYMVAS+RFTKNILVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420
           ATN+DGKLRYAINGVSH+DPETPLKLAEYFEVADKVFKYDTISDEG +KTAN VT+APNV
Sbjct: 361 ATNIDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480
           +NATFRNFVEI+FEN EKSLQSWHLDGYSFFAVA+EPGRWSPEKRKNYNLLDAVSRHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540
           VFPKSW AILLTLDNAGMWNLRSELTENRYLGQQ+YFSVQSPARSLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPPPYTI 553
           LVKDM LP PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of Cucsat.G14086 vs. ExPASy TrEMBL
Match: A0A1S3AX96 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103483330 PE=3 SV=1)

HSP 1 Score: 1090 bits (2820), Expect = 0.0
Identity = 521/553 (94.21%), Postives = 538/553 (97.29%), Query Frame = 0

Query: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLT +L+LCLL VAAM TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMH+A GAFGGLRVNSRLLIPVPY DPEDDYTVLIGDWYTKSHSTLKKFL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSHSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLING+IAKGDGTDEPLFTM P KTYKYRICNVGLK SLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKISLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTAD+DP+DYYMVAS+RFTKNILVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420
           ATN+DGKLRYAINGVSH+DPETPLKLAEYFEVADKVFKYDTISDEG +KTAN VT+APNV
Sbjct: 361 ATNIDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480
           +NATFRNFVEI+FEN EKSLQSWHLDGYSFFAVA+EPGRWSPEKRKNYNLLDAVSRHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540
           VFPKSW AILLTLDNAGMWNLRSELTENRYLGQQ+YFSVQSPARSLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPPPYTI 553
           LVKDM LP PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of Cucsat.G14086 vs. ExPASy TrEMBL
Match: A0A6J1DK02 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021193 PE=3 SV=1)

HSP 1 Score: 1044 bits (2699), Expect = 0.0
Identity = 493/553 (89.15%), Postives = 526/553 (95.12%), Query Frame = 0

Query: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MA L  +++LCL S AAM TVRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLMFTVLLCL-SAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NST+NNN+V+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMH+A G FGGLRVNSRLLIPVPYADPEDDYTVL+GDWYTKSHS LK FL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFL 180

Query: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRS+ARPDGVLING+ AKGDGTDEPLFTMKPEKTYKYR+CN+GLKTSLN R Q HTMK
Sbjct: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300
           LVEMEGSH VQN YQSLD+HVGQCFSVLVTA+++PKDYY++ASTRFTKN+LVGKGIIRYT
Sbjct: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300

Query: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           NGK PASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKI+NS
Sbjct: 301 NGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360

Query: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420
           AT VDGKLRYAINGVSH+DPETPLKLAEYF +ADKVFKYDTISD+G ++ AN VTVAPNV
Sbjct: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNV 420

Query: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480
           +NATFRNFVEIVFEN EKSLQSWHL+GYSFFAVA+EPGRW+PEKRKNYNLLDAVSR TIQ
Sbjct: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQ+YFSV SPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPPPYTI 553
           LVKDMPLP PYTI
Sbjct: 541 LVKDMPLPKPYTI 552

BLAST of Cucsat.G14086 vs. ExPASy TrEMBL
Match: A0A5A7TDG2 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G001010 PE=3 SV=1)

HSP 1 Score: 1040 bits (2689), Expect = 0.0
Identity = 493/553 (89.15%), Postives = 523/553 (94.58%), Query Frame = 0

Query: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARL  + +LCL + A M TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NST+NNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTNFTYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMH+A G FGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK++L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLING+ AKGDGTDEPLFTMKP KTYKYR+CNVGLK+SLNFR QGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300
           LVEMEGSHTVQN YQSLDVHVGQCFSVLVTA+++ KDYYMVASTRF KN+LVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360
           +GK PASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420
           A+ VDGKLRYAINGVSHVDPETPLKLAEYF +ADKVFKYDTISDEGL++ A  VTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480
           +NATFR+F+EI+FEN EKSLQSWHLDGYSFFAVA+EPGRWSPEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DNAGMWNLRSELTENRYLG Q+Y SV SPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPPPYTI 553
           LVKDMP+P PYTI
Sbjct: 541 LVKDMPMPKPYTI 553

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q006246.2e-25473.99L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291625.4e-23467.45L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU403.5e-15649.63Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q8VXX52.4e-14946.77Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN64.6e-14846.20Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
XP_004137383.10.0100.00L-ascorbate oxidase homolog [Cucumis sativus] >KAE8652494.1 hypothetical protein... [more]
KAA0036016.10.094.39L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
XP_008438536.10.094.21PREDICTED: L-ascorbate oxidase homolog [Cucumis melo] >TYJ98816.1 L-ascorbate ox... [more]
XP_022153749.10.089.15L-ascorbate oxidase homolog [Momordica charantia][more]
XP_011654310.20.088.79L-ascorbate oxidase homolog [Cucumis sativus] >KGN54106.2 hypothetical protein C... [more]
Match NameE-valueIdentityDescription
A0A5A7T1B70.094.39L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3BIA50.094.21L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3AX960.094.21L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103483330 PE=3 SV=1[more]
A0A6J1DK020.089.15L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021193 PE=3 ... [more]
A0A5A7TDG20.089.15L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 157..337
e-value: 2.2E-36
score: 127.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 347..545
e-value: 1.8E-36
score: 127.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 29..148
e-value: 6.3E-33
score: 115.6
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 156..338
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 24..173
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 349..539
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 382..520
e-value: 5.1E-25
score: 87.8
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 159..299
e-value: 7.1E-40
score: 136.7
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 33..147
e-value: 3.6E-39
score: 133.5
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 5..544
NoneNo IPR availablePANTHERPTHR11709:SF246L-ASCORBATE OXIDASE-LIKE PROTEINcoord: 5..544
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 159..299
e-value: 9.109E-86
score: 260.413
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 29..145
e-value: 8.36704E-81
score: 246.55

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G14086.T1Cucsat.G14086.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity