Cucsat.G13810 (gene) Cucumber (B10) v3

BLAST of Cucsat.G13810 vs. ExPASy Swiss-Prot
Match: Q9ZQC5 (Interactor of constitutive active ROPs 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICR2 PE=1 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 8.5e-93
Identity = 267/631 (42.31%), Postives = 383/631 (60.70%), Query Frame = 0

Query: 71  MQTSKPK--SLE------PLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVV- 130
           MQT KP+  SLE      P   P+  R+LK+  SD VS   SP    +  K ++PK V  
Sbjct: 1   MQTPKPRPGSLEVPQKKSPASTPKTARKLKTSESDPVS---SPNTKIRTPKTQSPKVVAD 60

Query: 131 TKSVQSSV--LEKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQR 190
            +S ++ V  ++KKR  +   + SQI+QLQ+ELKK K+QL++SE+ K+ A+++AEE K  
Sbjct: 61  RRSPRTPVNEIQKKRTGKTPELASQISQLQEELKKAKEQLSASEALKKEAQDQAEETK-- 120

Query: 191 LIVMSSELEDSRQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAA 250
                       QQL +++ASED RI ELRK+SQ+RD+AWQSELEA+Q+QH MDSAAL++
Sbjct: 121 ------------QQLMEINASEDSRIDELRKLSQERDKAWQSELEAMQRQHAMDSAALSS 180

Query: 251 AMHENQKLKLQLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDS 310
            M+E QKLK QL               S    ++ LR+EL ETLSLVE+LR +L + ++ 
Sbjct: 181 TMNEVQKLKAQL---------------SESENVENLRMELNETLSLVEKLRGELFDAKEG 240

Query: 311 EAQALEDLKKTHMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKY 370
           EAQA E +  T  +L+ AN T+E L+S+G    +A +S++ ELEQSK +V +LE LV   
Sbjct: 241 EAQAHEIVSGTEKQLEIANLTLEMLRSDGMKMSEACNSLTTELEQSKSEVRSLEQLV--- 300

Query: 371 QDDFAEIDRKKLEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQ 430
                    ++LE+  E + N   D ++  + +LK E+N  R E+ QL+ A++  +RRY 
Sbjct: 301 ---------RQLEEEDEARGNANGDSSS--VEELKEEINVARQEISQLKSAVEVTERRYH 360

Query: 431 DEYLRSTIQIRSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIA 490
           +EY++ST+QIR++YE+V+ +KS   ++EA    EL + KA+   L   L DKEA+L  + 
Sbjct: 361 EEYIQSTLQIRTAYEQVDEVKSGYAQREAELGEELKKTKAERDSLHERLMDKEAKLRILV 420

Query: 491 KEKETASLNQKIKESEK------------ETDISEQLKKFESDIEELKASLLDKETELQG 550
            E E   LN KIKE E+            E + + +LKK ESD+ EL+A+L+DKE ELQ 
Sbjct: 421 DENEI--LNSKIKEKEEVYLNLENSLNQNEPEDTGELKKLESDVMELRANLMDKEMELQS 480

Query: 551 IIEENDMLRVGIQKMETERK-------IEHGETTDLEEPAKSANEETTNKLGSVN-ENSE 610
           ++ + + LR  ++ M++E+         + G  T+  + +    E  T +LG+    N+E
Sbjct: 481 VMSQYESLRSEMETMQSEKNKAIDEALAKLGSLTEEADKSGKRAENATEQLGAAQVTNTE 540

Query: 611 TEAELRRLRVQLDQWRKAAEAAAAMLSPG-----KDGKLVDIAGSIDS-----NYPLSSY 660
            EAELRRL+VQ DQWRKAAEAAA MLS G      +GK V+  GS++S     N  +S Y
Sbjct: 541 LEAELRRLKVQCDQWRKAAEAAATMLSGGNNNNNSNGKYVERTGSLESPLRRRNVNMSPY 583

BLAST of Cucsat.G13810 vs. ExPASy Swiss-Prot
Match: Q9LSS5 (Interactor of constitutive active ROPs 3 OS=Arabidopsis thaliana OX=3702 GN=ICR3 PE=1 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 6.6e-77
Identity = 230/605 (38.02%), Postives = 370/605 (61.16%), Query Frame = 0

Query: 71  MQTSKPKSLEP----LRKPRPIRQLKSPGSDSVSASVSPLPPSKMT-KERNPKTVVTKSV 130
           MQT K ++  P       PR  R LK    +  S+S SP+  +  T K+++P  +  +S 
Sbjct: 1   MQTQKARNGSPDVPKKVSPRAARPLKIAALEPESSS-SPISATNRTPKDKSPNVLNRRSP 60

Query: 131 QSSVLEKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSS 190
           +S V EKKRP+R++ +E  ++QLQ+ELKK KDQ++ SE+ K++A++EAEE++++L  +SS
Sbjct: 61  RSPVSEKKRPSRITELELLVSQLQEELKKAKDQISVSETSKKQAEQEAEESRKQLQEVSS 120

Query: 191 ELEDSRQQLFDLSASEDERIQE----LRKVSQDRDRAWQSELEALQKQHLMDSAALAAAM 250
           +LE+S+ Q  + SA E+E  +      + VSQ+ D  W+    A ++      A LAA  
Sbjct: 121 KLEESQNQFVETSALEEETDKTGSLVFQSVSQECD--WEFSATAGER------AGLAAVA 180

Query: 251 HENQKLKLQLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEA 310
           HE ++LKLQ+E +A SEA   + AE  ++E+Q LR  L +TL  VE  R++L + E SEA
Sbjct: 181 HEIRQLKLQIEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEA 240

Query: 311 QALEDLKKTHMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQD 370
           +      +T  +L+ A K +E L+S+GT A++++  +++ELEQSK ++  LEALV+K Q+
Sbjct: 241 ETEALATETLRQLENAKKAVEELKSDGTKAVESYKKMAVELEQSKSRMVWLEALVNKLQN 300

Query: 371 DFAEIDRKK--LEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQ 430
           + A+++  +  L+D+   +  +         N++  E++ +R E+ +LR AL+A+D++ Q
Sbjct: 301 NPADLENHEILLKDYESLRRGES--------NEMDEEVSSLRCEVERLRAALEASDKKDQ 360

Query: 431 DEYLRSTIQIRSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIA 490
           +  + ++ ++R                +A  ++EL  AK++I +L+  L DKE +L  I+
Sbjct: 361 EGNVEASSRLRI---------------QAELQSELKIAKSEIDELKARLMDKETELQFIS 420

Query: 491 KEKETASLNQKIKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGI 550
           +E++  S+  K+ +++KE D+  +LKK    IE LKA L+DKETELQ + +EN+ L+  I
Sbjct: 421 EERDNFSM--KLMKNQKEIDVEAELKKLREAIENLKADLMDKETELQIVSDENETLKSDI 480

Query: 551 QKMETERK---IEHG-ETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWR 610
            K ET+ +   ++ G    + ++ +K A   T     +   NSE E ELR+L+VQ +QWR
Sbjct: 481 HKSETDVQDAFLKLGIAMEEADKSSKKAVRVTEQLEATQASNSEMETELRKLKVQSNQWR 540

Query: 611 KAAEAAAAMLSPGKDGKLVDIAGSIDSNY-PLSSYYSEDLDDDSPKKKNINMLKKIGVLW 660
           KAAEAA AMLS G +GK  +       NY   +S YSED+DD+  KKKN N+LKKIGVLW
Sbjct: 541 KAAEAATAMLSAGNNGKFAE-------NYNQTNSPYSEDIDDELTKKKNGNVLKKIGVLW 564

BLAST of Cucsat.G13810 vs. ExPASy Swiss-Prot
Match: Q8VYU8 (Interactor of constitutive active ROPs 5 OS=Arabidopsis thaliana OX=3702 GN=ICR5 PE=1 SV=2)

HSP 1 Score: 187.2 bits (474), Expect = 6.0e-46
Identity = 200/599 (33.39%), Postives = 284/599 (47.41%), Query Frame = 0

Query: 71  MQT--SKPKSLE------PLRKPRPIRQLKSPG--SDSVSASVSPLPPSKMTKERNPKTV 130
           MQT  S+P SLE      PL  P+ +R+LK  G  SD  + ++S     K+  +R    +
Sbjct: 1   MQTPKSRPGSLELPQKKSPLPAPKVVRRLKPSGAESDPKTKTISKTQIPKVVADRRSARI 60

Query: 131 VTKSVQSSVLEKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRL 190
               +Q     KKR  R+  +ES I+QLQ+ELKK K++LN SE+ KR A+EEAE+AK   
Sbjct: 61  PLNEIQ-----KKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAK--- 120

Query: 191 IVMSSELEDSRQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAA 250
                       QL D++ASED RI+ELRK+SQ+RD+ WQSELEA+Q+QH MDS AL++A
Sbjct: 121 -----------HQLMDINASEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALSSA 180

Query: 251 MHENQKLKLQLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSE 310
           ++E QKLK                                          SKL E E   
Sbjct: 181 INEVQKLK------------------------------------------SKLFESES-- 240

Query: 311 AQALEDLKKTHMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQ 370
                                                   ELEQSK +V +LE LV    
Sbjct: 241 ----------------------------------------ELEQSKYEVRSLEKLV---- 300

Query: 371 DDFAEIDRKKLEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQD 430
                   ++LE   E + N +D  ++  + +LK  +N  R E+ QL+ A++AA+ RYQ+
Sbjct: 301 --------RQLE---EERVNSRDSSSSMEVEELKEAMNLSRQEITQLKSAVEAAETRYQE 360

Query: 431 EYLRSTIQIRSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAK 490
           EY++ST+QIRS+YE+ E++KS   ++EA    EL + K +I+ LR               
Sbjct: 361 EYIQSTLQIRSAYEQTEAVKSRYSQREAELTEELNRTKDEIEGLR--------------- 396

Query: 491 EKETASLNQKIKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQ 550
                 L +K+KE E   D    LKK ESD+ E++ SL+DKE ELQ            I 
Sbjct: 421 ----KELMEKVKEDESTGD----LKKLESDLMEVRGSLMDKEMELQ------------IL 396

Query: 551 KMETERKIEHGETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEA 610
           +   E+K+E            +AN E              EAEL+R+++Q +QWRKAAE 
Sbjct: 481 RSAMEKKVE------------TANTEA------------MEAELKRVKIQCEQWRKAAET 396

Query: 611 AAAMLSPGKDGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 660
           AA++L+   D +  D   SI+++                      MLKK GVL KK+ K
Sbjct: 541 AASILN--NDEERTD---SIETS---------------------KMLKKFGVLLKKNHK 396

BLAST of Cucsat.G13810 vs. ExPASy Swiss-Prot
Match: F4I8B9 (Putative WEB family protein At1g65010, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g65010 PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 2.5e-04
Identity = 126/560 (22.50%), Postives = 255/560 (45.54%), Query Frame = 0

Query: 85  PRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSVLEKKRPNRVST---- 144
           P P R  K   S S S S SP+P ++++ +R+P TV +K         +RP+R+ T    
Sbjct: 19  PPPPRLSKLSASKSDSNSASPVPNTRLSLDRSPPTVNSKPTPD-----RRPSRIPTPEKV 78

Query: 145 ---------IESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELEDSR 204
                    +++Q+ Q+Q++LKK  +Q+   +  K +A ++ +E+++ +   + +L+++ 
Sbjct: 79  HSRLVKGTELQTQLNQIQEDLKKADEQIELLKKDKAKAIDDLKESEKLVEEANEKLKEAL 138

Query: 205 QQLFDLSASEDERIQELRKVS---------QDRDRAWQSELEALQKQHLMDSAALAAAMH 264
                  A E   +++ R V          Q +D   ++ELE+++ QH +D +AL +   
Sbjct: 139 AA--QKRAEESFEVEKFRAVELEQAGLEAVQKKDVTSKNELESIRSQHALDISALLSTTE 198

Query: 265 ENQKLKLQLERIAGSEANQSRHAESAHAEIQGLRIELKETL-SLVEELRSKLSEYEDSEA 324
           E Q++K +L   A ++     HAE A  +I  +  E  E L S +  L++ L   E+ EA
Sbjct: 199 ELQRVKHELSMTADAKNKALSHAEEA-TKIAEIHAEKAEILASELGRLKALLGSKEEKEA 258

Query: 325 -QALEDLKKTHMELQTANKTIEN---LQSEGTNAMKAFSSISLELEQSKEKVTTLEALVS 384
            +  E + K   E++     +E    L+S           + ++LE +K   +   + V 
Sbjct: 259 IEGNEIVSKLKSEIELLRGELEKVSILESSLKEQEGLVEQLKVDLEAAKMAESCTNSSVE 318

Query: 385 KYQDDFAEIDRKKLEDHSENKNNDKDD---------EANEYINQLKNELNCVRSEMGQLR 444
           ++++   E++ K++E+ + +K++  +          E N  +++ K++ N  + E  +L 
Sbjct: 319 EWKNKVHELE-KEVEESNRSKSSASESMESVMKQLAELNHVLHETKSD-NAAQKEKIELL 378

Query: 445 LALDAADRRYQDEYLRSTIQIRSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHL 504
                A R   +EY R     +    ++E+L    + +    + E  +A    K   +++
Sbjct: 379 EKTIEAQRTDLEEYGRQVCIAKEEASKLENLVESIKSELEISQEEKTRALDNEKAATSNI 438

Query: 505 EDKEAQLLSIAKEKETASLNQKIKESEKETDISEQLKKFESDIEELKASLLDKETELQGI 564
           ++   Q   ++ E E   + ++  + + E+ ++  L++  ++  E KA+LL  + EL+  
Sbjct: 439 QNLLDQRTELSIELERCKVEEEKSKKDMES-LTLALQEASTESSEAKATLLVCQEELKNC 498

Query: 565 IEENDMLRVGIQKMETERKIE------HGETTDLEEPAKS-ANEETTNKLG--------- 588
             + D L+  +   ET  K E        E   L+    S  NE   +K G         
Sbjct: 499 ESQVDSLK--LASKETNEKYEKMLEDARNEIDSLKSTVDSIQNEFENSKAGWEQKELHLM 558

BLAST of Cucsat.G13810 vs. NCBI nr
Match: XP_011653597.1 (interactor of constitutive active ROPs 2, chloroplastic [Cucumis sativus] >XP_011653599.1 interactor of constitutive active ROPs 2, chloroplastic [Cucumis sativus] >XP_011653600.1 interactor of constitutive active ROPs 2, chloroplastic [Cucumis sativus] >XP_011653602.1 interactor of constitutive active ROPs 2, chloroplastic [Cucumis sativus] >XP_031740135.1 interactor of constitutive active ROPs 2, chloroplastic [Cucumis sativus] >KGN54185.1 hypothetical protein Csa_018051 [Cucumis sativus])

HSP 1 Score: 1046 bits (2705), Expect = 0.0
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 71  MQTSKPKSLEPLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSVL 130
           MQTSKPKSLEPLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSVL
Sbjct: 1   MQTSKPKSLEPLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSVL 60

Query: 131 EKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELEDS 190
           EKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELEDS
Sbjct: 61  EKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELEDS 120

Query: 191 RQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKLQ 250
           RQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKLQ
Sbjct: 121 RQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKLQ 180

Query: 251 LERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKKT 310
           LERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKKT
Sbjct: 181 LERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKKT 240

Query: 311 HMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAEIDRKK 370
           HMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAEIDRKK
Sbjct: 241 HMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAEIDRKK 300

Query: 371 LEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQIR 430
           LEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQIR
Sbjct: 301 LEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQIR 360

Query: 431 SSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETASLNQK 490
           SSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETASLNQK
Sbjct: 361 SSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETASLNQK 420

Query: 491 IKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIEH 550
           IKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIEH
Sbjct: 421 IKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIEH 480

Query: 551 GETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGKD 610
           GETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGKD
Sbjct: 481 GETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGKD 540

Query: 611 GKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 659
           GKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 541 GKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 589

BLAST of Cucsat.G13810 vs. NCBI nr
Match: KAA0047284.1 (interactor of constitutive active ROPs 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1029 bits (2661), Expect = 0.0
Identity = 582/620 (93.87%), Postives = 600/620 (96.77%), Query Frame = 0

Query: 41  ERNSCGSESFEGEHQLFIQPAFILQQELQSMQTSKPKSLE-PLRKPRPIRQLKSPGSDSV 100
           ++++   +SFEGE+QLFIQPAFILQ+ELQ+MQT KP+SLE PLRKPRPIRQLKSPG+DSV
Sbjct: 13  QQSTVKEKSFEGEYQLFIQPAFILQRELQNMQTPKPRSLEAPLRKPRPIRQLKSPGTDSV 72

Query: 101 SASVSPLPPSKMTKERNPKTVVTKSVQSSVLEKKRPNRVSTIESQIAQLQDELKKTKDQL 160
           SASVSPLPPSKMTKER+PKTV  KSVQSSVLEKKRPNRVST+ESQIAQLQDELKKTKDQL
Sbjct: 73  SASVSPLPPSKMTKERSPKTVA-KSVQSSVLEKKRPNRVSTVESQIAQLQDELKKTKDQL 132

Query: 161 NSSESGKRRAKEEAEEAKQRLIVMSSELEDSRQQLFDLSASEDERIQELRKVSQDRDRAW 220
           NSSESGKRRAKEEAEEAKQRLIVMSSELEDSRQQL +LSASEDERIQELRK+SQDRDRAW
Sbjct: 133 NSSESGKRRAKEEAEEAKQRLIVMSSELEDSRQQLLELSASEDERIQELRKISQDRDRAW 192

Query: 221 QSELEALQKQHLMDSAALAAAMHENQKLKLQLERIAGSEANQSRHAESAHAEIQGLRIEL 280
           QSELEALQKQHLMDSAAL AAMHENQKLKLQLERIAGSEANQSRHAESAHAEIQGLRIEL
Sbjct: 193 QSELEALQKQHLMDSAALGAAMHENQKLKLQLERIAGSEANQSRHAESAHAEIQGLRIEL 252

Query: 281 KETLSLVEELRSKLSEYEDSEAQALEDLKKTHMELQTANKTIENLQSEGTNAMKAFSSIS 340
           KETLSLVEELRSKLSEYEDSEAQALEDLKKT MEL+TANKTIE LQSEGTNAMKAFSSIS
Sbjct: 253 KETLSLVEELRSKLSEYEDSEAQALEDLKKTQMELETANKTIERLQSEGTNAMKAFSSIS 312

Query: 341 LELEQSKEKVTTLEALVSKYQDDFAEIDRKKLEDHSENKNNDKDDEANEYINQLKNELNC 400
           LELEQSKEKVTTLEALVSKYQDD AEID+K LEDHSENKNNDKDDE NEYINQL NELNC
Sbjct: 313 LELEQSKEKVTTLEALVSKYQDDLAEIDKKNLEDHSENKNNDKDDEENEYINQLNNELNC 372

Query: 401 VRSEMGQLRLALDAADRRYQDEYLRSTIQIRSSYEEVESLKSESRRKEAAFEAELIQAKA 460
           VRSEMGQLRLALDAADRRYQDEYLRSTIQIRSSYEEVESLKSESRRKEAAFEAELIQAK 
Sbjct: 373 VRSEMGQLRLALDAADRRYQDEYLRSTIQIRSSYEEVESLKSESRRKEAAFEAELIQAKE 432

Query: 461 QIKQLRTHLEDKEAQLLSIAKEKETASLNQKIKESEKETDISEQLKKFESDIEELKASLL 520
           QIKQLRTHLEDKEAQL+S+AKEKETASLNQK KESE ETDISEQLKKFESDIEELKASLL
Sbjct: 433 QIKQLRTHLEDKEAQLMSMAKEKETASLNQKSKESEIETDISEQLKKFESDIEELKASLL 492

Query: 521 DKETELQGIIEENDMLRVGIQKMETERKIEHGETTDLEEPAKSANEETTNKLGSVNENSE 580
           DKETELQGIIEENDMLRVGIQKMETERKIEHGETTDLEEP KSANEET NKLGSVNENSE
Sbjct: 493 DKETELQGIIEENDMLRVGIQKMETERKIEHGETTDLEEPTKSANEETLNKLGSVNENSE 552

Query: 581 TEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVDIAGSIDSNYPLSSYYSEDLDDDSP 640
           TEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVDIAGSIDSNYPLSSYYSEDLDDDSP
Sbjct: 553 TEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVDIAGSIDSNYPLSSYYSEDLDDDSP 612

Query: 641 KKKNINMLKKIGVLWKKSQK 659
           KKKNINMLKKIGVLWKKSQK
Sbjct: 613 KKKNINMLKKIGVLWKKSQK 631

BLAST of Cucsat.G13810 vs. NCBI nr
Match: XP_008449280.1 (PREDICTED: interactor of constitutive active ROPs 2, chloroplastic [Cucumis melo] >XP_008449282.1 PREDICTED: interactor of constitutive active ROPs 2, chloroplastic [Cucumis melo])

HSP 1 Score: 992 bits (2565), Expect = 0.0
Identity = 563/590 (95.42%), Postives = 573/590 (97.12%), Query Frame = 0

Query: 71  MQTSKPKSLE-PLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSV 130
           MQT KP+SLE PLRKPRPIRQLKSPG+DSVSASVSPLPPSKMTKER+PKTV  KSVQSSV
Sbjct: 1   MQTPKPRSLEAPLRKPRPIRQLKSPGTDSVSASVSPLPPSKMTKERSPKTVA-KSVQSSV 60

Query: 131 LEKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELED 190
           LEKKRPNRVST+ESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELED
Sbjct: 61  LEKKRPNRVSTVESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELED 120

Query: 191 SRQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKL 250
           SRQQL +LSASEDERIQELRK+SQDRDRAWQSELEALQKQHLMDSAAL AAMHENQKLKL
Sbjct: 121 SRQQLLELSASEDERIQELRKISQDRDRAWQSELEALQKQHLMDSAALGAAMHENQKLKL 180

Query: 251 QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKK 310
           QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKK
Sbjct: 181 QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKK 240

Query: 311 THMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAEIDRK 370
           T MEL+TANKTIE LQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDD AEID+K
Sbjct: 241 TQMELETANKTIERLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDLAEIDKK 300

Query: 371 KLEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI 430
            LEDHSENKNNDKDDE NEYINQL NELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI
Sbjct: 301 NLEDHSENKNNDKDDEENEYINQLNNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI 360

Query: 431 RSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETASLNQ 490
           RSSYEEVESLKSESRRKEAAFEAELIQAK QIKQLRTHLEDKEAQL+S+AKEKETASLNQ
Sbjct: 361 RSSYEEVESLKSESRRKEAAFEAELIQAKEQIKQLRTHLEDKEAQLMSMAKEKETASLNQ 420

Query: 491 KIKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIE 550
           K KESE ETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIE
Sbjct: 421 KSKESEIETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIE 480

Query: 551 HGETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK 610
           HGETTDLEEP KSANEET NKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK
Sbjct: 481 HGETTDLEEPTKSANEETLNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK 540

Query: 611 DGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 659
           DGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 541 DGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 589

BLAST of Cucsat.G13810 vs. NCBI nr
Match: XP_038882490.1 (interactor of constitutive active ROPs 2, chloroplastic [Benincasa hispida] >XP_038882491.1 interactor of constitutive active ROPs 2, chloroplastic [Benincasa hispida] >XP_038882492.1 interactor of constitutive active ROPs 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 949 bits (2454), Expect = 0.0
Identity = 534/590 (90.51%), Postives = 564/590 (95.59%), Query Frame = 0

Query: 71  MQTSKPKSLE-PLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSV 130
           MQTSKPKSLE PLRKPRP RQLK+PGSDSVS S SPLP SKMTKERNPKT+VTKSVQSSV
Sbjct: 1   MQTSKPKSLEAPLRKPRPTRQLKAPGSDSVSVSPSPLPSSKMTKERNPKTIVTKSVQSSV 60

Query: 131 LEKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELED 190
           LEKKRPNRVST+ESQIAQLQDELKK KDQLN+SESGKRRAKEEAEEAKQ+L  MSSEL+D
Sbjct: 61  LEKKRPNRVSTMESQIAQLQDELKKAKDQLNASESGKRRAKEEAEEAKQQLNAMSSELKD 120

Query: 191 SRQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKL 250
           SRQQL +LSASEDERIQELRK+SQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKL
Sbjct: 121 SRQQLLELSASEDERIQELRKISQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKL 180

Query: 251 QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKK 310
           QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEEL+SKLSEYEDSEAQA+EDLKK
Sbjct: 181 QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELKSKLSEYEDSEAQAVEDLKK 240

Query: 311 THMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAEIDRK 370
           T MEL+TAN+TIE LQSEGTNAMKAFSSISLELEQSKEKVT+LEALVSKYQ+D AEID+K
Sbjct: 241 TQMELETANETIEMLQSEGTNAMKAFSSISLELEQSKEKVTSLEALVSKYQNDLAEIDKK 300

Query: 371 KLEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI 430
            LEDHSENKNNDK+DE NEY+NQLK ELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI
Sbjct: 301 NLEDHSENKNNDKEDEENEYVNQLKTELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI 360

Query: 431 RSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETASLNQ 490
           RSSYEEVES+KSESRRKEAAFEAELIQAKAQI+QLRTHLEDKEAQLLSIAKE E  +LNQ
Sbjct: 361 RSSYEEVESIKSESRRKEAAFEAELIQAKAQIEQLRTHLEDKEAQLLSIAKENEIVNLNQ 420

Query: 491 KIKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIE 550
           K KE+E E+D++EQLKK E+DIE+LKASLLDKETELQGIIEENDMLRV IQKM TERKIE
Sbjct: 421 KSKETENESDLAEQLKKLEADIEDLKASLLDKETELQGIIEENDMLRVDIQKMGTERKIE 480

Query: 551 HGETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK 610
           HGETTDLEE A+SANEE  NKLGS+NENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK
Sbjct: 481 HGETTDLEESARSANEEALNKLGSINENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK 540

Query: 611 DGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 659
           DGKLVDI+GSIDSNYPLSS+YSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 541 DGKLVDISGSIDSNYPLSSFYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 590

BLAST of Cucsat.G13810 vs. NCBI nr
Match: KAG6604442.1 (Interactor of constitutive active ROPs 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 868 bits (2243), Expect = 6.97e-311
Identity = 500/616 (81.17%), Postives = 549/616 (89.12%), Query Frame = 0

Query: 45  CGSESFEGEHQLFIQPAFILQQELQSMQTSKPKSLE-PLRKPRPIRQLKSPGSDSVSASV 104
           CGSE FEG++QLFIQPAFILQQELQ+MQTSKPK+LE PLRKPRP RQLK+PGSDSVSA  
Sbjct: 40  CGSERFEGKYQLFIQPAFILQQELQNMQTSKPKNLEAPLRKPRPTRQLKAPGSDSVSAPA 99

Query: 105 SPLPPSKMTKERNPKTVVTKSVQSSVLEKKRPNRVSTIESQIAQLQDELKKTKDQLNSSE 164
           S                    VQSSVLEKKRPNRVST+ESQIAQLQDELKK KDQLNS+E
Sbjct: 100 S--------------------VQSSVLEKKRPNRVSTMESQIAQLQDELKKAKDQLNSAE 159

Query: 165 SGKRRAKEEAEEAKQRLIVMSSELEDSRQQLFDLSASEDERIQELRKVSQDRDRAWQSEL 224
            G++RAKEEAEE +Q+L  MS ELE+SRQQL +LSASEDERIQELRK+SQDRDRAWQSEL
Sbjct: 160 LGRKRAKEEAEEGRQQLEAMSLELEESRQQLLELSASEDERIQELRKISQDRDRAWQSEL 219

Query: 225 EALQKQHLMDSAALAAAMHENQKLKLQLERIAGSEANQSRHAESAHAEIQGLRIELKETL 284
           EALQKQHLMDSAAL AAM+ENQKLKLQLERIAG E N SRHAESA AEI  LR+ELKETL
Sbjct: 220 EALQKQHLMDSAALGAAMNENQKLKLQLERIAGLEVNHSRHAESAQAEIHSLRVELKETL 279

Query: 285 SLVEELRSKLSEYEDSEAQALEDLKKTHMELQTANKTIENLQSEGTNAMKAFSSISLELE 344
           SLVEEL+SKLSEYEDSEAQ++ED KKT MEL+TAN+TIE L+SEGTNAMKAFSSISLELE
Sbjct: 280 SLVEELKSKLSEYEDSEAQSVEDQKKTQMELETANETIEALRSEGTNAMKAFSSISLELE 339

Query: 345 QSKEKVTTLEALVSKYQDDFAEIDRKKLEDHSENKNNDKDDEANEYINQLKNELNCVRSE 404
           QSKEKV +LEALVSKYQ D AEID+K LED SENKNN+KD+E  EYINQLK ELNCVRSE
Sbjct: 340 QSKEKVASLEALVSKYQVDLAEIDKKNLEDQSENKNNEKDEEETEYINQLKTELNCVRSE 399

Query: 405 MGQLRLALDAADRRYQDEYLRSTIQIRSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQ 464
           MGQL+LALDAADRRYQDEYLRSTIQIRSSYEE+ES++SESR +EAAFEAEL++AKAQI+Q
Sbjct: 400 MGQLKLALDAADRRYQDEYLRSTIQIRSSYEELESVRSESRLREAAFEAELVRAKAQIEQ 459

Query: 465 LRTHLEDKEAQLLSIAKEKETASLNQKIKESEKETDISEQLKKFESDIEELKASLLDKET 524
           LRT LEDKEAQLLSIAKEKET++LNQ+ KESE+E+D +EQLKK E+DIEELKASLLDKET
Sbjct: 460 LRTDLEDKEAQLLSIAKEKETSNLNQRSKESERESDNTEQLKKLEADIEELKASLLDKET 519

Query: 525 ELQGIIEENDMLRVGIQKMETERKIEHGETTDLEEPAKSANEETTNKLGSVNENSETEAE 584
           ELQGIIEENDMLRV IQKMET RK+ +GETTDLEE   +ANEE+ NKLGSVNENSE EAE
Sbjct: 520 ELQGIIEENDMLRVDIQKMETGRKMANGETTDLEE---AANEESLNKLGSVNENSEMEAE 579

Query: 585 LRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKN 644
           LRRLRVQL+QWRKAAEAAAAMLSPGKDGKLVDI+G IDSNYP  S YSE+LDDDSPKKKN
Sbjct: 580 LRRLRVQLEQWRKAAEAAAAMLSPGKDGKLVDISGPIDSNYPHGSLYSEELDDDSPKKKN 632

Query: 645 INMLKKIGVLWKKSQK 659
           INMLKKIGVLWKK+QK
Sbjct: 640 INMLKKIGVLWKKNQK 632

BLAST of Cucsat.G13810 vs. ExPASy TrEMBL
Match: A0A0A0KX24 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G292460 PE=3 SV=1)

HSP 1 Score: 1046 bits (2705), Expect = 0.0
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 71  MQTSKPKSLEPLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSVL 130
           MQTSKPKSLEPLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSVL
Sbjct: 1   MQTSKPKSLEPLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSVL 60

Query: 131 EKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELEDS 190
           EKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELEDS
Sbjct: 61  EKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELEDS 120

Query: 191 RQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKLQ 250
           RQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKLQ
Sbjct: 121 RQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKLQ 180

Query: 251 LERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKKT 310
           LERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKKT
Sbjct: 181 LERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKKT 240

Query: 311 HMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAEIDRKK 370
           HMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAEIDRKK
Sbjct: 241 HMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAEIDRKK 300

Query: 371 LEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQIR 430
           LEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQIR
Sbjct: 301 LEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQIR 360

Query: 431 SSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETASLNQK 490
           SSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETASLNQK
Sbjct: 361 SSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETASLNQK 420

Query: 491 IKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIEH 550
           IKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIEH
Sbjct: 421 IKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIEH 480

Query: 551 GETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGKD 610
           GETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGKD
Sbjct: 481 GETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGKD 540

Query: 611 GKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 659
           GKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 541 GKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 589

BLAST of Cucsat.G13810 vs. ExPASy TrEMBL
Match: A0A5A7TWE3 (Interactor of constitutive active ROPs 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G00780 PE=3 SV=1)

HSP 1 Score: 1029 bits (2661), Expect = 0.0
Identity = 582/620 (93.87%), Postives = 600/620 (96.77%), Query Frame = 0

Query: 41  ERNSCGSESFEGEHQLFIQPAFILQQELQSMQTSKPKSLE-PLRKPRPIRQLKSPGSDSV 100
           ++++   +SFEGE+QLFIQPAFILQ+ELQ+MQT KP+SLE PLRKPRPIRQLKSPG+DSV
Sbjct: 13  QQSTVKEKSFEGEYQLFIQPAFILQRELQNMQTPKPRSLEAPLRKPRPIRQLKSPGTDSV 72

Query: 101 SASVSPLPPSKMTKERNPKTVVTKSVQSSVLEKKRPNRVSTIESQIAQLQDELKKTKDQL 160
           SASVSPLPPSKMTKER+PKTV  KSVQSSVLEKKRPNRVST+ESQIAQLQDELKKTKDQL
Sbjct: 73  SASVSPLPPSKMTKERSPKTVA-KSVQSSVLEKKRPNRVSTVESQIAQLQDELKKTKDQL 132

Query: 161 NSSESGKRRAKEEAEEAKQRLIVMSSELEDSRQQLFDLSASEDERIQELRKVSQDRDRAW 220
           NSSESGKRRAKEEAEEAKQRLIVMSSELEDSRQQL +LSASEDERIQELRK+SQDRDRAW
Sbjct: 133 NSSESGKRRAKEEAEEAKQRLIVMSSELEDSRQQLLELSASEDERIQELRKISQDRDRAW 192

Query: 221 QSELEALQKQHLMDSAALAAAMHENQKLKLQLERIAGSEANQSRHAESAHAEIQGLRIEL 280
           QSELEALQKQHLMDSAAL AAMHENQKLKLQLERIAGSEANQSRHAESAHAEIQGLRIEL
Sbjct: 193 QSELEALQKQHLMDSAALGAAMHENQKLKLQLERIAGSEANQSRHAESAHAEIQGLRIEL 252

Query: 281 KETLSLVEELRSKLSEYEDSEAQALEDLKKTHMELQTANKTIENLQSEGTNAMKAFSSIS 340
           KETLSLVEELRSKLSEYEDSEAQALEDLKKT MEL+TANKTIE LQSEGTNAMKAFSSIS
Sbjct: 253 KETLSLVEELRSKLSEYEDSEAQALEDLKKTQMELETANKTIERLQSEGTNAMKAFSSIS 312

Query: 341 LELEQSKEKVTTLEALVSKYQDDFAEIDRKKLEDHSENKNNDKDDEANEYINQLKNELNC 400
           LELEQSKEKVTTLEALVSKYQDD AEID+K LEDHSENKNNDKDDE NEYINQL NELNC
Sbjct: 313 LELEQSKEKVTTLEALVSKYQDDLAEIDKKNLEDHSENKNNDKDDEENEYINQLNNELNC 372

Query: 401 VRSEMGQLRLALDAADRRYQDEYLRSTIQIRSSYEEVESLKSESRRKEAAFEAELIQAKA 460
           VRSEMGQLRLALDAADRRYQDEYLRSTIQIRSSYEEVESLKSESRRKEAAFEAELIQAK 
Sbjct: 373 VRSEMGQLRLALDAADRRYQDEYLRSTIQIRSSYEEVESLKSESRRKEAAFEAELIQAKE 432

Query: 461 QIKQLRTHLEDKEAQLLSIAKEKETASLNQKIKESEKETDISEQLKKFESDIEELKASLL 520
           QIKQLRTHLEDKEAQL+S+AKEKETASLNQK KESE ETDISEQLKKFESDIEELKASLL
Sbjct: 433 QIKQLRTHLEDKEAQLMSMAKEKETASLNQKSKESEIETDISEQLKKFESDIEELKASLL 492

Query: 521 DKETELQGIIEENDMLRVGIQKMETERKIEHGETTDLEEPAKSANEETTNKLGSVNENSE 580
           DKETELQGIIEENDMLRVGIQKMETERKIEHGETTDLEEP KSANEET NKLGSVNENSE
Sbjct: 493 DKETELQGIIEENDMLRVGIQKMETERKIEHGETTDLEEPTKSANEETLNKLGSVNENSE 552

Query: 581 TEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVDIAGSIDSNYPLSSYYSEDLDDDSP 640
           TEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVDIAGSIDSNYPLSSYYSEDLDDDSP
Sbjct: 553 TEAELRRLRVQLDQWRKAAEAAAAMLSPGKDGKLVDIAGSIDSNYPLSSYYSEDLDDDSP 612

Query: 641 KKKNINMLKKIGVLWKKSQK 659
           KKKNINMLKKIGVLWKKSQK
Sbjct: 613 KKKNINMLKKIGVLWKKSQK 631

BLAST of Cucsat.G13810 vs. ExPASy TrEMBL
Match: A0A1S3BLP1 (interactor of constitutive active ROPs 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491205 PE=3 SV=1)

HSP 1 Score: 992 bits (2565), Expect = 0.0
Identity = 563/590 (95.42%), Postives = 573/590 (97.12%), Query Frame = 0

Query: 71  MQTSKPKSLE-PLRKPRPIRQLKSPGSDSVSASVSPLPPSKMTKERNPKTVVTKSVQSSV 130
           MQT KP+SLE PLRKPRPIRQLKSPG+DSVSASVSPLPPSKMTKER+PKTV  KSVQSSV
Sbjct: 1   MQTPKPRSLEAPLRKPRPIRQLKSPGTDSVSASVSPLPPSKMTKERSPKTVA-KSVQSSV 60

Query: 131 LEKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELED 190
           LEKKRPNRVST+ESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELED
Sbjct: 61  LEKKRPNRVSTVESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSSELED 120

Query: 191 SRQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQKLKL 250
           SRQQL +LSASEDERIQELRK+SQDRDRAWQSELEALQKQHLMDSAAL AAMHENQKLKL
Sbjct: 121 SRQQLLELSASEDERIQELRKISQDRDRAWQSELEALQKQHLMDSAALGAAMHENQKLKL 180

Query: 251 QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKK 310
           QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKK
Sbjct: 181 QLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALEDLKK 240

Query: 311 THMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAEIDRK 370
           T MEL+TANKTIE LQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDD AEID+K
Sbjct: 241 TQMELETANKTIERLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDLAEIDKK 300

Query: 371 KLEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI 430
            LEDHSENKNNDKDDE NEYINQL NELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI
Sbjct: 301 NLEDHSENKNNDKDDEENEYINQLNNELNCVRSEMGQLRLALDAADRRYQDEYLRSTIQI 360

Query: 431 RSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETASLNQ 490
           RSSYEEVESLKSESRRKEAAFEAELIQAK QIKQLRTHLEDKEAQL+S+AKEKETASLNQ
Sbjct: 361 RSSYEEVESLKSESRRKEAAFEAELIQAKEQIKQLRTHLEDKEAQLMSMAKEKETASLNQ 420

Query: 491 KIKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIE 550
           K KESE ETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIE
Sbjct: 421 KSKESEIETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETERKIE 480

Query: 551 HGETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK 610
           HGETTDLEEP KSANEET NKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK
Sbjct: 481 HGETTDLEEPTKSANEETLNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAMLSPGK 540

Query: 611 DGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 659
           DGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK
Sbjct: 541 DGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 589

BLAST of Cucsat.G13810 vs. ExPASy TrEMBL
Match: A0A6J1IL28 (interactor of constitutive active ROPs 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478014 PE=3 SV=1)

HSP 1 Score: 862 bits (2226), Expect = 2.75e-309
Identity = 496/594 (83.50%), Postives = 542/594 (91.25%), Query Frame = 0

Query: 71  MQTSKPKSLE-PLRKPRPIRQLKSPGSDSVSASVS----PLPPSKMTKERNPKTVVTKSV 130
           MQTSKPK+LE PLRKPRP RQLK+ GSDSVSA  S    PLPPSKMTKER+P+ +VTKSV
Sbjct: 1   MQTSKPKNLEAPLRKPRPTRQLKALGSDSVSAPASALGSPLPPSKMTKERSPR-IVTKSV 60

Query: 131 QSSVLEKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSS 190
           QSSVLEKKRPNRVST+ESQIAQLQDELKK KDQLNS+E G+RRAKEEAEE +Q+L  MS 
Sbjct: 61  QSSVLEKKRPNRVSTVESQIAQLQDELKKAKDQLNSAELGRRRAKEEAEEGRQQLEAMSL 120

Query: 191 ELEDSRQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQ 250
           ELE+SRQQL +LSASEDERIQELRK+SQDRDR WQSELEALQKQHLMDSAAL AAM+ENQ
Sbjct: 121 ELEESRQQLLELSASEDERIQELRKISQDRDREWQSELEALQKQHLMDSAALGAAMNENQ 180

Query: 251 KLKLQLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALE 310
           KLKLQLERIAG E N SRHAESA AEI  LR+ELKETLSLVEEL+SKLSEYEDSEAQ+LE
Sbjct: 181 KLKLQLERIAGLEVNHSRHAESAQAEIHSLRVELKETLSLVEELKSKLSEYEDSEAQSLE 240

Query: 311 DLKKTHMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAE 370
           D KKT MEL+ AN+TIE LQSEGTNAMKAFSSISLELEQSKEKV +LEALVSKYQ D AE
Sbjct: 241 DQKKTQMELERANETIEMLQSEGTNAMKAFSSISLELEQSKEKVASLEALVSKYQVDLAE 300

Query: 371 IDRKKLEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRS 430
           ID+K LED SENKNN+KD+E  EYI+QLK ELNCVRSEMGQL+LALDAADRRYQDEYLRS
Sbjct: 301 IDKKNLEDQSENKNNEKDEEETEYISQLKTELNCVRSEMGQLKLALDAADRRYQDEYLRS 360

Query: 431 TIQIRSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETA 490
           TIQIRSSYEE+ES++SESR +EAAFEAEL++AKAQI+QLRTHLEDKEAQLLSIAKEKET+
Sbjct: 361 TIQIRSSYEELESVRSESRLREAAFEAELVRAKAQIEQLRTHLEDKEAQLLSIAKEKETS 420

Query: 491 SLNQKIKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETE 550
           +LNQ+ KESE+E+D +EQLKK E+DIEELKASLLDKETELQGIIEENDMLRV IQKMET 
Sbjct: 421 NLNQRSKESERESDNTEQLKKLEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETG 480

Query: 551 RKIEHGETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAML 610
           RK+ +GETTDLEEPA   NEET NKLGSVNENSE EAELRRLRVQL+QWRKAAEAAAAML
Sbjct: 481 RKMANGETTDLEEPA---NEETLNKLGSVNENSEMEAELRRLRVQLEQWRKAAEAAAAML 540

Query: 611 SPGKDGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 659
           SPGKDGKLVDI+G IDSNYP  S+YSE+LDDDSPKKKNINMLKKIGVLWKK+QK
Sbjct: 541 SPGKDGKLVDISGPIDSNYPHGSFYSEELDDDSPKKKNINMLKKIGVLWKKNQK 590

BLAST of Cucsat.G13810 vs. ExPASy TrEMBL
Match: A0A6J1EDK6 (interactor of constitutive active ROPs 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433198 PE=3 SV=1)

HSP 1 Score: 858 bits (2216), Expect = 9.46e-308
Identity = 493/594 (83.00%), Postives = 541/594 (91.08%), Query Frame = 0

Query: 71  MQTSKPKSLE-PLRKPRPIRQLKSPGSDSVSASVSP----LPPSKMTKERNPKTVVTKSV 130
           MQTSKPK+LE PLRKPRPIRQLK+PGSDSVSA  S     LPPSKMTKER+P+ VVTKSV
Sbjct: 1   MQTSKPKNLEAPLRKPRPIRQLKAPGSDSVSAPASASGSLLPPSKMTKERSPRPVVTKSV 60

Query: 131 QSSVLEKKRPNRVSTIESQIAQLQDELKKTKDQLNSSESGKRRAKEEAEEAKQRLIVMSS 190
           QSSVLEKKRPNRVST+ESQIAQLQDELKK KDQLNS+E G+RRAKEEAEE +Q+L  MS 
Sbjct: 61  QSSVLEKKRPNRVSTMESQIAQLQDELKKAKDQLNSAELGRRRAKEEAEEGRQQLEAMSL 120

Query: 191 ELEDSRQQLFDLSASEDERIQELRKVSQDRDRAWQSELEALQKQHLMDSAALAAAMHENQ 250
           ELE+SRQQL +LSASEDERIQELRK+SQDRDRAWQSELEALQKQHLMDSAAL AAM+ENQ
Sbjct: 121 ELEESRQQLLELSASEDERIQELRKISQDRDRAWQSELEALQKQHLMDSAALGAAMNENQ 180

Query: 251 KLKLQLERIAGSEANQSRHAESAHAEIQGLRIELKETLSLVEELRSKLSEYEDSEAQALE 310
           KLKLQLERIAG E N SRHAESA AEI  LR+ELKETLSLVEEL+SKLSEYEDSEAQ++E
Sbjct: 181 KLKLQLERIAGLEVNHSRHAESAQAEIHSLRVELKETLSLVEELKSKLSEYEDSEAQSVE 240

Query: 311 DLKKTHMELQTANKTIENLQSEGTNAMKAFSSISLELEQSKEKVTTLEALVSKYQDDFAE 370
           D KKT MEL+TAN+TIE L+SEGTNAMKAFSSISLELEQSKEKV +LEALVSKYQ D AE
Sbjct: 241 DQKKTQMELETANETIEALRSEGTNAMKAFSSISLELEQSKEKVASLEALVSKYQVDLAE 300

Query: 371 IDRKKLEDHSENKNNDKDDEANEYINQLKNELNCVRSEMGQLRLALDAADRRYQDEYLRS 430
           ID+K LED SENKNN+KD+E  EYINQLK ELNCVRSEMGQL+LALDAADRRYQDEYLRS
Sbjct: 301 IDKKNLEDQSENKNNEKDEEETEYINQLKTELNCVRSEMGQLKLALDAADRRYQDEYLRS 360

Query: 431 TIQIRSSYEEVESLKSESRRKEAAFEAELIQAKAQIKQLRTHLEDKEAQLLSIAKEKETA 490
           TIQIRSSYEE+ES++SESR +EAAFEAEL++AKAQI+QLRT L DKE +LLSIAKEKET+
Sbjct: 361 TIQIRSSYEELESVRSESRLREAAFEAELVRAKAQIEQLRTDLVDKETRLLSIAKEKETS 420

Query: 491 SLNQKIKESEKETDISEQLKKFESDIEELKASLLDKETELQGIIEENDMLRVGIQKMETE 550
           +LNQ+ KESE+E+D +EQLKK E+DIEELKASLLDKETELQGIIEENDMLRV IQKMET 
Sbjct: 421 NLNQRSKESERESDNTEQLKKLEADIEELKASLLDKETELQGIIEENDMLRVDIQKMETG 480

Query: 551 RKIEHGETTDLEEPAKSANEETTNKLGSVNENSETEAELRRLRVQLDQWRKAAEAAAAML 610
           RK+ +GETT+LEEPA   NEET NKLGSVNENSE EAELRRLRVQL+QWRKAAEAAAAML
Sbjct: 481 RKMTNGETTNLEEPA---NEETLNKLGSVNENSEMEAELRRLRVQLEQWRKAAEAAAAML 540

Query: 611 SPGKDGKLVDIAGSIDSNYPLSSYYSEDLDDDSPKKKNINMLKKIGVLWKKSQK 659
           SPGKDGKLVDI+G ID+NYP  S YSE+LDDDSPKKKNINMLKKIGVLWKK+QK
Sbjct: 541 SPGKDGKLVDISGPIDTNYPHGSLYSEELDDDSPKKKNINMLKKIGVLWKKNQK 591