Cucsat.G13147 (gene) Cucumber (B10) v3

Overview
NameCucsat.G13147
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionstrigolactone esterase D14-like
Locationctg1838: 1043247 .. 1044919 (+)
RNA-Seq ExpressionCucsat.G13147
SyntenyCucsat.G13147
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTTTTTTTAACTTTTGAGTGGAAAAACTACTATAAATACACAAGGAATAGAACTAGTGTATATCTCAACACTCCAAAGCCTTAATTTATCCACATTCCATTCAATCTCCATATCCTAAAAAAAACCATCAAAATGTATAATGGATTCAATTGTGGAGGTGGATCAGCGATTGCCAAAGCTCTCAATGTCAATGTGTATGGCAATGGCAGCGAGACACTCGTGTTGGCTCACGGATTCGGGTTGGATCAAAATGTTTGGCATTACATGGTTCCCTACCTTGCTTGTTTCTTCAAAGTGGTTGTTTTTGACTTGGTTTTTTCTCCAAATGTGAAGCTTGAGCTATATGATGAGAGGAAGTACTCAAGTTTTGATGGATATGCCAAAGATCTGTTATGTGTTCTTGACCATCTCCATGTTAAGAGAACTATTTACGTTGGTCATTCCATGTCCGCTATGGTTGGATGTGTTGCTGCAACCTATAGGCCTCAACTCTTTCACCATCTTGTATTGTTGAATGCCTCTCCAAGGTAATTCCCCTCTAACACTCTCCCTTTTGTTTTGGTTATCATTGAGAAGAATGAAACAATATAAAACCAAACAAAAAACCAAGTCCACGGAAAGAACTCCAATATATAAAAGAAAGGTTTTCAGAATATTGAAAATTATACTACGCGAGAGGTTGAAATTTTGTTTTTAAGCGAAAAACTTACTATGTGTATATAAACATAGTTGGAATAATTAAGGAATGTGCTTTTTCCTCAAGATCAAATCAGTTTTTCTTTGTCTTCAAAAATATTAAAATTCAAGTGCTTTTAATGGGTTAAGATCATCGAAAACGTTTAATATTCATGATTTTTCTTGATTATTGTTTTATTAACTTTTTTGGTCTTTAGGTATCTCAATGGTGAAGGATATATTGGGGGATTTGAAAAGCCACAAGTTGATGAACTTTTGAAAGAAATGGATAAAAACTTCACAAAATGGGCAAAACAATTTGCTCCATTTGCTGTGATGGTGAACAATTCTAAAGCAATGACAGAAGTTGAGCATTCTTTGGGAAGAATGAACCCAAAAATTGCTCTAACTGTTGCAAAAATGGTGTTCTTAAGTGATTTAACAAAGCTTCTACCAAAAGTAAAAACTTCAACCTCCATTATTCTTACAAAAAAAGACAACATTGTCCCAAAATCAGTTGCATTTTTCATAAAATCAAATATTGGTGGTAATTCAAATGTTAACATATTGAAATCTCAAGGCCACTTCCCTCAACTAACAGCTTTTCCTCAACTTTTGAAAGTTTTGACTAAAGTTCTTCAACTCAAACAGTACAAGATCATCAACAATTGACAAATTGATTCAGCTCGCGTTATCGTTCGTGGTATCGCTAATTGATCAAATATATTCTATGGAGTATTATTGTGTGTTTGTCAATCCATTTGTGTAAGATTTTCACATGAAGTTATAGGTTTGTTTGAAGCACTATTAAGTGATGAAGTAATTTATTTTCATATTTTGGTTGTGTAAAGTTAACATCATTATGGTTTGACCACAGCTTTCTTGGACTCAGCTATATATATATATTTATTACATTAGAAAAAAAGGATGTCTAAAAAAGAGTATGAATTTAATGTGTTTATTTTCAAATGCTATATTCAATAATATTATTCATC

Coding sequence (CDS)

ATGTATAATGGATTCAATTGTGGAGGTGGATCAGCGATTGCCAAAGCTCTCAATGTCAATGTGTATGGCAATGGCAGCGAGACACTCGTGTTGGCTCACGGATTCGGGTTGGATCAAAATGTTTGGCATTACATGGTTCCCTACCTTGCTTGTTTCTTCAAAGTGGTTGTTTTTGACTTGGTTTTTTCTCCAAATGTGAAGCTTGAGCTATATGATGAGAGGAAGTACTCAAGTTTTGATGGATATGCCAAAGATCTGTTATGTGTTCTTGACCATCTCCATGTTAAGAGAACTATTTACGTTGGTCATTCCATGTCCGCTATGGTTGGATGTGTTGCTGCAACCTATAGGCCTCAACTCTTTCACCATCTTGTATTGTTGAATGCCTCTCCAAGGTATCTCAATGGTGAAGGATATATTGGGGGATTTGAAAAGCCACAAGTTGATGAACTTTTGAAAGAAATGGATAAAAACTTCACAAAATGGGCAAAACAATTTGCTCCATTTGCTGTGATGGTGAACAATTCTAAAGCAATGACAGAAGTTGAGCATTCTTTGGGAAGAATGAACCCAAAAATTGCTCTAACTGTTGCAAAAATGGTGTTCTTAAGTGATTTAACAAAGCTTCTACCAAAAGTAAAAACTTCAACCTCCATTATTCTTACAAAAAAAGACAACATTGTCCCAAAATCAGTTGCATTTTTCATAAAATCAAATATTGGTGGTAATTCAAATGTTAACATATTGAAATCTCAAGGCCACTTCCCTCAACTAACAGCTTTTCCTCAACTTTTGAAAGTTTTGACTAAAGTTCTTCAACTCAAACAGTACAAGATCATCAACAATTGA

Protein sequence

MYNGFNCGGGSAIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDLVFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQLFHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMTEVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNIGGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLKQYKIINN
Homology
BLAST of Cucsat.G13147 vs. ExPASy Swiss-Prot
Match: Q9SQR3 (Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 7.2e-57
Identity = 112/260 (43.08%), Postives = 168/260 (64.62%), Query Frame = 0

Query: 13  IAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDLVFSPNVKLELYD 72
           I +ALNV V G G   L LAHGFG DQ+ WH ++PY    ++VV++DLV + +V  + +D
Sbjct: 6   ILEALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSVNPDYFD 65

Query: 73  ERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQLFHHLVLLNASPR 132
             +Y++ D Y  DLL ++D L ++   YVGHS+SAM+G +A+  RP+LF  L+L+  SPR
Sbjct: 66  FNRYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILIGFSPR 125

Query: 133 YLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMTEVEHSLGRMNPK 192
           +LN E Y GGFE+ +++++   M+ N+  W   FAP AV  +   A+ E   +L  M P 
Sbjct: 126 FLNDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLFNMRPD 185

Query: 193 IALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNIGGNSNVNILKSQ 252
           I+L V++ VF SDL  +L  V+  T +I T KD  VP SVA +++S++GG++ V  LK++
Sbjct: 186 ISLFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVETLKTE 245

Query: 253 GHFPQLTAFPQLLKVLTKVL 273
           GH PQL+A  QL + L + L
Sbjct: 246 GHLPQLSAPAQLAQFLRRAL 265

BLAST of Cucsat.G13147 vs. ExPASy Swiss-Prot
Match: A0A109QYD3 (Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 6.7e-55
Identity = 112/252 (44.44%), Postives = 159/252 (63.10%), Query Frame = 0

Query: 10  GSAIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDLVFSPNVKLE 69
           G+ I  A NV V G+G + LV AHGFG DQ+ W  ++PY    +KV+++DLV + +V  +
Sbjct: 2   GTPILDAFNVRVEGSGDKYLVFAHGFGTDQSAWQRVLPYFTRSYKVILYDLVCAGSVNPD 61

Query: 70  LYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQLFHHLVLLNA 129
            +D R+Y++ D Y  DLL +LD LHV R  YVGHS+SAM G +A+  RP+LF  L+L+ A
Sbjct: 62  HFDFRRYTTLDAYVDDLLNILDSLHVTRCAYVGHSISAMTGMLASIRRPELFSKLILIGA 121

Query: 130 SPRYLN-GEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMTEVEHSLGR 189
           SPR+LN GE Y GGFE+ +++ +   M+ N+  W   FAP AV  +   A+ E   +L  
Sbjct: 122 SPRFLNDGENYHGGFEQGEIEHVFSAMEANYEAWVNGFAPLAVGADVPTAVREFSRTLFN 181

Query: 190 MNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNIGGNSNVNI 249
           M P I+L V++ VF SDL  +L  V     I+ T +D  VP SVA ++K +IGG S V  
Sbjct: 182 MRPDISLFVSRTVFNSDLRGILGLVNVPCCIMQTARDMSVPASVATYMKEHIGGKSTVQW 241

Query: 250 LKSQGHFPQLTA 261
           L ++GH P L+A
Sbjct: 242 LDTEGHLPHLSA 253

BLAST of Cucsat.G13147 vs. ExPASy Swiss-Prot
Match: J9U5U9 (Probable strigolactone esterase DAD2 OS=Petunia hybrida OX=4102 GN=DAD2 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 9.7e-54
Identity = 109/263 (41.44%), Postives = 165/263 (62.74%), Query Frame = 0

Query: 10  GSAIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDLVFSPNVKLE 69
           G  +  ALNV V G+G   LVLAHGFG DQ+ W+ ++P+    ++VV++DLV + +V  +
Sbjct: 2   GQTLLDALNVRVVGSGERVLVLAHGFGTDQSAWNRILPFFLRDYRVVLYDLVCAGSVNPD 61

Query: 70  LYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQLFHHLVLLNA 129
            +D R+Y++ D Y  DLL +LD L +    YVGHS+SAM+G +A+  RP+LF  L+L+ A
Sbjct: 62  FFDFRRYTTLDPYVDDLLHILDALGIDCCAYVGHSVSAMIGILASIRRPELFSKLILIGA 121

Query: 130 SPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMTEVEHSLGRM 189
           SPR+LN E Y GGFE+ +++++   M+ N+  W   FAP AV  +   A+ E   +L  M
Sbjct: 122 SPRFLNDEDYHGGFEQGEIEKVFSAMEANYEAWVNGFAPLAVGADVPAAVREFSRTLFNM 181

Query: 190 NPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNIGGNSNVNIL 249
            P I L V++ VF SD+  +L  VK    I  T +D+ VP SVA ++K+++GG + V+ L
Sbjct: 182 RPDITLFVSRTVFNSDMRGVLGLVKVPCHIFQTARDHSVPASVATYLKNHLGGKNTVHWL 241

Query: 250 KSQGHFPQLTAFPQLLKVLTKVL 273
             +GH P L+A   L + L + L
Sbjct: 242 NIEGHLPHLSAPTLLAQELRRAL 264

BLAST of Cucsat.G13147 vs. ExPASy Swiss-Prot
Match: Q10QA5 (Strigolactone esterase D14 OS=Oryza sativa subsp. japonica OX=39947 GN=D14 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 8.2e-53
Identity = 108/270 (40.00%), Postives = 166/270 (61.48%), Query Frame = 0

Query: 3   NGFNCGGGSAIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDLVF 62
           +G     G+ + + LNV V G+G   +VL+HGFG DQ+ W  ++PYL    +VV++DLV 
Sbjct: 46  SGSAAPSGAKLLQILNVRVVGSGERVVVLSHGFGTDQSAWSRVLPYLTRDHRVVLYDLVC 105

Query: 63  SPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQLFH 122
           + +V  + +D R+Y + D Y  DLL +LD L + R  +VGHS+SAM+G +A+  RP LF 
Sbjct: 106 AGSVNPDHFDFRRYDNLDAYVDDLLAILDALRIPRCAFVGHSVSAMIGILASIRRPDLFA 165

Query: 123 HLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMTEV 182
            LVL+ ASPR+LN   Y GGFE  ++ ++   M  N++ WA  +AP AV  +   A+ E 
Sbjct: 166 KLVLIGASPRFLNDSDYHGGFELEEIQQVFDAMGANYSAWATGYAPLAVGADVPAAVQEF 225

Query: 183 EHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNIGG 242
             +L  M P I+L V + VF +DL  +L  V+    ++ T +D  VP SVA ++K+++GG
Sbjct: 226 SRTLFNMRPDISLHVCQTVFKTDLRGVLGMVRAPCVVVQTTRDVSVPASVAAYLKAHLGG 285

Query: 243 NSNVNILKSQGHFPQLTAFPQLLKVLTKVL 273
            + V  L+++GH P L+A   L +VL + L
Sbjct: 286 RTTVEFLQTEGHLPHLSAPSLLAQVLRRAL 315

BLAST of Cucsat.G13147 vs. ExPASy Swiss-Prot
Match: Q10J20 (Probable esterase D14L OS=Oryza sativa subsp. japonica OX=39947 GN=D14L PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.4e-52
Identity = 110/263 (41.83%), Postives = 165/263 (62.74%), Query Frame = 0

Query: 13  IAKALNVNVYGNGSE-TLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDLVFSPNVKLELY 72
           + +A N+ V G G    +VLAHGFG DQ+VW ++VP+L   ++VV+FD + +     + +
Sbjct: 4   VEEAHNLRVVGEGKRGVIVLAHGFGTDQSVWKHLVPHLVADYRVVLFDTMGAGPTNPDYF 63

Query: 73  DERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQLFHHLVLLNASP 132
           D  +Y++ +GYA DLL +L  L V   IYVGHS+SA++G +A+  RP LF  LVLL+ASP
Sbjct: 64  DFSRYATLEGYALDLLAILQELRVASCIYVGHSVSAVIGAIASISRPDLFSKLVLLSASP 123

Query: 133 RYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVN-NSKAMTEVEHSLGRMN 192
           RYLN   Y GGFE+  +DEL + M  N+  W   FAP  V  +  S A+ E   +L  + 
Sbjct: 124 RYLNDVDYYGGFEQEDLDELFEAMGSNYKAWCSGFAPLCVGGDMESVAVQEFSRTLFNIR 183

Query: 193 PKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNIGGNSNVNILK 252
           P IAL+VA+ +F SD+  LLP V     I+ + KD  VP  V+ ++  ++GG+S V ++ 
Sbjct: 184 PDIALSVAQTIFQSDVRSLLPLVTVPCHIVQSTKDLAVPVVVSEYLHKHLGGDSIVEVMP 243

Query: 253 SQGHFPQLTAFPQLLKVLTKVLQ 274
           S+GH PQL++   ++ VL + +Q
Sbjct: 244 SEGHLPQLSSPDIVIPVLLRHIQ 266

BLAST of Cucsat.G13147 vs. NCBI nr
Match: XP_004136551.1 (strigolactone esterase D14 [Cucumis sativus] >XP_031744317.1 strigolactone esterase D14-like [Cucumis sativus] >KAE8645776.1 hypothetical protein Csa_017185 [Cucumis sativus] >KGN59265.1 hypothetical protein Csa_001825 [Cucumis sativus])

HSP 1 Score: 564 bits (1453), Expect = 1.15e-202
Identity = 281/282 (99.65%), Postives = 282/282 (100.00%), Query Frame = 0

Query: 1   MYNGFNCGGGSAIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL 60
           MYNGFNCGGGSAIA+ALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL
Sbjct: 1   MYNGFNCGGGSAIAEALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL 60

Query: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120
           VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT 180
           FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT
Sbjct: 121 FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT 180

Query: 181 EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI 240
           EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI
Sbjct: 181 EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI 240

Query: 241 GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLKQYKIINN 282
           GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLKQYKIINN
Sbjct: 241 GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLKQYKIINN 282

BLAST of Cucsat.G13147 vs. NCBI nr
Match: XP_008443004.1 (PREDICTED: strigolactone esterase D14-like [Cucumis melo] >KAA0043762.1 strigolactone esterase D14-like [Cucumis melo var. makuwa] >TYK25371.1 strigolactone esterase D14-like [Cucumis melo var. makuwa])

HSP 1 Score: 535 bits (1378), Expect = 3.47e-191
Identity = 263/279 (94.27%), Postives = 273/279 (97.85%), Query Frame = 0

Query: 1   MYNGFNCGGGSAIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL 60
           M NGFNC GGSAIA+AL VNVYGNG+ETLVLAHGFGLDQNVWHYM+PYLACFFKVVVFDL
Sbjct: 1   MNNGFNCRGGSAIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVVVFDL 60

Query: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120
           VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT 180
           FHHL+LLNASPRYLNGEGYIGGFEKPQVDE+ KEMDKNFTKWAKQF PFAV+VNNSKAM 
Sbjct: 121 FHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNSKAMA 180

Query: 181 EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI 240
           E+EHSLGRMNPKIAL+VAKMVFLSDLTKLLPKVKTSTSIILT+KDNIVPKSVAFFIKSNI
Sbjct: 181 EIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFIKSNI 240

Query: 241 GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLKQYKI 279
           GGNSNVNILKSQGHFPQLTA+PQLLKVLTKVLQLKQYKI
Sbjct: 241 GGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLKQYKI 279

BLAST of Cucsat.G13147 vs. NCBI nr
Match: XP_038905280.1 (strigolactone esterase D14-like [Benincasa hispida])

HSP 1 Score: 496 bits (1278), Expect = 6.48e-176
Identity = 239/278 (85.97%), Postives = 260/278 (93.53%), Query Frame = 0

Query: 1   MYNGFNCGGGSAIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL 60
           MY GFNCG GSA A+AL VNVYGNG+ETLVLAHGFG DQ+ WHYM+PYLACFFKVVVFDL
Sbjct: 1   MYGGFNCGAGSAFAEALKVNVYGNGTETLVLAHGFGSDQSAWHYMIPYLACFFKVVVFDL 60

Query: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120
           VFSPNVKLELYDERKYSSFDGYAKDL+CVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLVCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT 180
           FHHL+LLNASPRYLN EGY GGF++PQVDE+ K+MD NFTKW K+FAPFAV VN+SKAM 
Sbjct: 121 FHHLILLNASPRYLNAEGYFGGFKRPQVDEIFKQMDNNFTKWVKEFAPFAVRVNDSKAMA 180

Query: 181 EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI 240
           E EHSLGRMNPKIAL VAKMVFLSDL KLLPKV+T TSIILT+KDNIVPKSVAFF+KSN+
Sbjct: 181 EFEHSLGRMNPKIALNVAKMVFLSDLRKLLPKVRTPTSIILTEKDNIVPKSVAFFMKSNM 240

Query: 241 GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLKQYK 278
           GGNSNV ILKS+GHFPQLTA+PQLLKVL+KVL+LK+YK
Sbjct: 241 GGNSNVKILKSEGHFPQLTAYPQLLKVLSKVLKLKEYK 278

BLAST of Cucsat.G13147 vs. NCBI nr
Match: XP_022945470.1 (strigolactone esterase D14-like [Cucurbita moschata])

HSP 1 Score: 431 bits (1108), Expect = 4.54e-150
Identity = 210/278 (75.54%), Postives = 240/278 (86.33%), Query Frame = 0

Query: 1   MYNGFNCGGGS---AIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVV 60
           M  GFNCGG S   AIA+AL VNVYG G +TLVLAHGFGLDQ+ W YM+PYLACFFKVVV
Sbjct: 1   MVGGFNCGGSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVV 60

Query: 61  FDLVFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYR 120
           FDLVFSPNVK E+YDERKYSSF+GYAKDL+CVLDHLHV+R+IYVGHSMSAM+GCVAATYR
Sbjct: 61  FDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYR 120

Query: 121 PQLFHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSK 180
           PQLF HL+LLNASPRYLNGEGY GGFE+P VD +  +MDKNFT W K FAPFAV +N+ K
Sbjct: 121 PQLFQHLILLNASPRYLNGEGYNGGFERPVVDAIFNQMDKNFTNWVKNFAPFAVSINDPK 180

Query: 181 AMTEVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIK 240
            + E+EHSLGRM P+IAL+VAKMVFLSDL +LLPKVKT T+II T+KDNIVPK VAF+IK
Sbjct: 181 TIAELEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVAFYIK 240

Query: 241 SNIGGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLK 275
           S +G ++ V IL S GHFPQLTA+PQLLKVL +VL L+
Sbjct: 241 SKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLQ 278

BLAST of Cucsat.G13147 vs. NCBI nr
Match: KAG6596658.1 (Strigolactone esterase D14, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028196.1 Strigolactone esterase D14, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 428 bits (1100), Expect = 7.78e-149
Identity = 210/279 (75.27%), Postives = 238/279 (85.30%), Query Frame = 0

Query: 1   MYNGFNCGGGS----AIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVV 60
           M  GFNCGG S    AIA+AL VNVYG G +TLVLAHGFGLDQ+ W YM+PYLACFFKVV
Sbjct: 1   MVGGFNCGGSSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVV 60

Query: 61  VFDLVFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATY 120
           VFDLVFSPNVK E+YDERKYSSF+GYAKDL+CVLDHLHV+R+IYVGHSMSAM+GCVAATY
Sbjct: 61  VFDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATY 120

Query: 121 RPQLFHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNS 180
           RPQLF HL+LLNASPRYLNGEGY GGFE+P VD +  +MDKNFT W K FAPFAV +N+ 
Sbjct: 121 RPQLFQHLILLNASPRYLNGEGYNGGFERPIVDAIFNQMDKNFTNWVKNFAPFAVSINDP 180

Query: 181 KAMTEVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFI 240
           K + E EHSLGRM P+IAL+VAKMVFLSDL +LLPKVKT T+II T+KDNIVPK VA +I
Sbjct: 181 KTIAEFEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVASYI 240

Query: 241 KSNIGGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLK 275
           KS +G ++ V IL S GHFPQLTA+PQLLKVL +VL LK
Sbjct: 241 KSKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLK 279

BLAST of Cucsat.G13147 vs. ExPASy TrEMBL
Match: A0A0A0LB72 (Sigma factor sigb regulation protein rsbq OS=Cucumis sativus OX=3659 GN=Csa_3G792050 PE=4 SV=1)

HSP 1 Score: 564 bits (1453), Expect = 5.55e-203
Identity = 281/282 (99.65%), Postives = 282/282 (100.00%), Query Frame = 0

Query: 1   MYNGFNCGGGSAIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL 60
           MYNGFNCGGGSAIA+ALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL
Sbjct: 1   MYNGFNCGGGSAIAEALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL 60

Query: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120
           VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT 180
           FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT
Sbjct: 121 FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT 180

Query: 181 EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI 240
           EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI
Sbjct: 181 EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI 240

Query: 241 GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLKQYKIINN 282
           GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLKQYKIINN
Sbjct: 241 GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLKQYKIINN 282

BLAST of Cucsat.G13147 vs. ExPASy TrEMBL
Match: A0A5A7TKE6 (Strigolactone esterase D14-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005110 PE=4 SV=1)

HSP 1 Score: 535 bits (1378), Expect = 1.68e-191
Identity = 263/279 (94.27%), Postives = 273/279 (97.85%), Query Frame = 0

Query: 1   MYNGFNCGGGSAIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL 60
           M NGFNC GGSAIA+AL VNVYGNG+ETLVLAHGFGLDQNVWHYM+PYLACFFKVVVFDL
Sbjct: 1   MNNGFNCRGGSAIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVVVFDL 60

Query: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120
           VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT 180
           FHHL+LLNASPRYLNGEGYIGGFEKPQVDE+ KEMDKNFTKWAKQF PFAV+VNNSKAM 
Sbjct: 121 FHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNSKAMA 180

Query: 181 EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI 240
           E+EHSLGRMNPKIAL+VAKMVFLSDLTKLLPKVKTSTSIILT+KDNIVPKSVAFFIKSNI
Sbjct: 181 EIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFIKSNI 240

Query: 241 GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLKQYKI 279
           GGNSNVNILKSQGHFPQLTA+PQLLKVLTKVLQLKQYKI
Sbjct: 241 GGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLKQYKI 279

BLAST of Cucsat.G13147 vs. ExPASy TrEMBL
Match: A0A1S3B719 (strigolactone esterase D14-like OS=Cucumis melo OX=3656 GN=LOC103486734 PE=4 SV=1)

HSP 1 Score: 535 bits (1378), Expect = 1.68e-191
Identity = 263/279 (94.27%), Postives = 273/279 (97.85%), Query Frame = 0

Query: 1   MYNGFNCGGGSAIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFDL 60
           M NGFNC GGSAIA+AL VNVYGNG+ETLVLAHGFGLDQNVWHYM+PYLACFFKVVVFDL
Sbjct: 1   MNNGFNCRGGSAIAEALKVNVYGNGTETLVLAHGFGLDQNVWHYMIPYLACFFKVVVFDL 60

Query: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120
           VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL
Sbjct: 61  VFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQL 120

Query: 121 FHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAMT 180
           FHHL+LLNASPRYLNGEGYIGGFEKPQVDE+ KEMDKNFTKWAKQF PFAV+VNNSKAM 
Sbjct: 121 FHHLILLNASPRYLNGEGYIGGFEKPQVDEIFKEMDKNFTKWAKQFVPFAVIVNNSKAMA 180

Query: 181 EVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSNI 240
           E+EHSLGRMNPKIAL+VAKMVFLSDLTKLLPKVKTSTSIILT+KDNIVPKSVAFFIKSNI
Sbjct: 181 EIEHSLGRMNPKIALSVAKMVFLSDLTKLLPKVKTSTSIILTEKDNIVPKSVAFFIKSNI 240

Query: 241 GGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLKQYKI 279
           GGNSNVNILKSQGHFPQLTA+PQLLKVLTKVLQLKQYKI
Sbjct: 241 GGNSNVNILKSQGHFPQLTAYPQLLKVLTKVLQLKQYKI 279

BLAST of Cucsat.G13147 vs. ExPASy TrEMBL
Match: A0A6J1G109 (strigolactone esterase D14-like OS=Cucurbita moschata OX=3662 GN=LOC111449693 PE=4 SV=1)

HSP 1 Score: 431 bits (1108), Expect = 2.20e-150
Identity = 210/278 (75.54%), Postives = 240/278 (86.33%), Query Frame = 0

Query: 1   MYNGFNCGGGS---AIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVV 60
           M  GFNCGG S   AIA+AL VNVYG G +TLVLAHGFGLDQ+ W YM+PYLACFFKVVV
Sbjct: 1   MVGGFNCGGSSSAAAIAEALKVNVYGEGMDTLVLAHGFGLDQSAWRYMIPYLACFFKVVV 60

Query: 61  FDLVFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYR 120
           FDLVFSPNVK E+YDERKYSSF+GYAKDL+CVLDHLHV+R+IYVGHSMSAM+GCVAATYR
Sbjct: 61  FDLVFSPNVKPEVYDERKYSSFEGYAKDLVCVLDHLHVERSIYVGHSMSAMIGCVAATYR 120

Query: 121 PQLFHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSK 180
           PQLF HL+LLNASPRYLNGEGY GGFE+P VD +  +MDKNFT W K FAPFAV +N+ K
Sbjct: 121 PQLFQHLILLNASPRYLNGEGYNGGFERPVVDAIFNQMDKNFTNWVKNFAPFAVSINDPK 180

Query: 181 AMTEVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIK 240
            + E+EHSLGRM P+IAL+VAKMVFLSDL +LLPKVKT T+II T+KDNIVPK VAF+IK
Sbjct: 181 TIAELEHSLGRMKPEIALSVAKMVFLSDLRQLLPKVKTPTTIIPTRKDNIVPKFVAFYIK 240

Query: 241 SNIGGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLK 275
           S +G ++ V IL S GHFPQLTA+PQLLKVL +VL L+
Sbjct: 241 SKVGCHAKVKILNSDGHFPQLTAYPQLLKVLAQVLHLQ 278

BLAST of Cucsat.G13147 vs. ExPASy TrEMBL
Match: A0A6J1DC02 (strigolactone esterase D14-like OS=Momordica charantia OX=3673 GN=LOC111019326 PE=4 SV=1)

HSP 1 Score: 427 bits (1098), Expect = 7.07e-149
Identity = 205/276 (74.28%), Postives = 248/276 (89.86%), Query Frame = 0

Query: 1   MYNGF-NCGGGSAIAKALNVNVYGNGSETLVLAHGFGLDQNVWHYMVPYLACFFKVVVFD 60
           M+ GF NCG  +AI +ALNVNVYGNG+ T+VLAHGFGLDQ+VW Y++PYLACFFKVVVFD
Sbjct: 1   MHGGFFNCGE-AAIVEALNVNVYGNGTATVVLAHGFGLDQSVWRYVIPYLACFFKVVVFD 60

Query: 61  LVFSPNVKLELYDERKYSSFDGYAKDLLCVLDHLHVKRTIYVGHSMSAMVGCVAATYRPQ 120
            +FSPNVK ELYDE+KYSSFDGYA+DL+CVL+HLHV+R+IYVGHSMSAM+GCVAATYRPQ
Sbjct: 61  SIFSPNVKHELYDEKKYSSFDGYARDLVCVLEHLHVERSIYVGHSMSAMIGCVAATYRPQ 120

Query: 121 LFHHLVLLNASPRYLNGEGYIGGFEKPQVDELLKEMDKNFTKWAKQFAPFAVMVNNSKAM 180
           LF HL+LLNASPRY+N +GY GGFE+PQ+D + K+MD+NFT W K FAPFAV VN+SKA+
Sbjct: 121 LFTHLILLNASPRYINDKGYYGGFERPQLDGIFKQMDQNFTNWVKNFAPFAVAVNDSKAI 180

Query: 181 TEVEHSLGRMNPKIALTVAKMVFLSDLTKLLPKVKTSTSIILTKKDNIVPKSVAFFIKSN 240
           +E EHSLGRM PKIAL+VAK VFLSDL +LLPKV+T ++IILT+KDNIVPKSVAF++KS 
Sbjct: 181 SEFEHSLGRMKPKIALSVAKKVFLSDLRRLLPKVRTPSTIILTRKDNIVPKSVAFYMKSK 240

Query: 241 IGGNSNVNILKSQGHFPQLTAFPQLLKVLTKVLQLK 275
           +GG++NV IL S+GHFPQL A+PQ LKVL++VL+LK
Sbjct: 241 MGGHANVKILNSEGHFPQLIAYPQFLKVLSQVLRLK 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SQR37.2e-5743.08Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1[more]
A0A109QYD36.7e-5544.44Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1[more]
J9U5U99.7e-5441.44Probable strigolactone esterase DAD2 OS=Petunia hybrida OX=4102 GN=DAD2 PE=1 SV=... [more]
Q10QA58.2e-5340.00Strigolactone esterase D14 OS=Oryza sativa subsp. japonica OX=39947 GN=D14 PE=1 ... [more]
Q10J202.4e-5241.83Probable esterase D14L OS=Oryza sativa subsp. japonica OX=39947 GN=D14L PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_004136551.11.15e-20299.65strigolactone esterase D14 [Cucumis sativus] >XP_031744317.1 strigolactone ester... [more]
XP_008443004.13.47e-19194.27PREDICTED: strigolactone esterase D14-like [Cucumis melo] >KAA0043762.1 strigola... [more]
XP_038905280.16.48e-17685.97strigolactone esterase D14-like [Benincasa hispida][more]
XP_022945470.14.54e-15075.54strigolactone esterase D14-like [Cucurbita moschata][more]
KAG6596658.17.78e-14975.27Strigolactone esterase D14, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
Match NameE-valueIdentityDescription
A0A0A0LB725.55e-20399.65Sigma factor sigb regulation protein rsbq OS=Cucumis sativus OX=3659 GN=Csa_3G79... [more]
A0A5A7TKE61.68e-19194.27Strigolactone esterase D14-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3B7191.68e-19194.27strigolactone esterase D14-like OS=Cucumis melo OX=3656 GN=LOC103486734 PE=4 SV=... [more]
A0A6J1G1092.20e-15075.54strigolactone esterase D14-like OS=Cucurbita moschata OX=3662 GN=LOC111449693 PE... [more]
A0A6J1DC027.07e-14974.28strigolactone esterase D14-like OS=Momordica charantia OX=3673 GN=LOC111019326 P... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 8..273
e-value: 1.1E-54
score: 187.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 17..272
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 28..135
e-value: 3.8E-10
score: 39.8
NoneNo IPR availablePANTHERPTHR43039ESTERASE-RELATEDcoord: 6..274
NoneNo IPR availablePANTHERPTHR43039:SF1SIGMA FACTOR SIGB REGULATION PROTEIN RSBQcoord: 6..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G13147.T1Cucsat.G13147.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity