Cucsat.G12896 (gene) Cucumber (B10) v3

Overview
NameCucsat.G12896
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionEndoglucanase
Locationctg1838: 6751566 .. 6756051 (-)
RNA-Seq ExpressionCucsat.G12896
SyntenyCucsat.G12896
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAATATAAAAATGAAAAAAATGTTACATCGTGAAATTTGATAAAACAAAATTTGTCGGTGACGAAGCTCTTTTCTTGCACACCAAAAATCACATAGACATATAAGTCAGAAGTCCTTTCCTCTCCCCTTGGCGTCTGGCCCAGATCCAACTTCCCCCATTTCTTTAATTAGGGTTTGTTTCAATAACTTCCAATCTCGACCAATCATTTCCTTAATCACACTTCTCTTTAGGAAACAGATCTTGATTTGTCACCCTTTTTTTTTCTATCACTTCCAGATTTCCACCTACCCTGTTATAGACCCCTATATTGGAATGCTCCCCATTGTCGCAATGTATAGAGTTTGAACTAGATCTTGGTTCTTTCCTTATTCTGAGCTTTGCATTTACGCAATAATAGAGATTCACATTGCGCAAAATGGGGGAGAAATCGAGGTCTAGAGGCTGTTGTGGATGGTTTCTTGTTTTGGTGGTTCTTGCTTTGATTGCTGGTGCTATAGTATTTGCAGTCAAGAAGAAGTCGAGTCACTCTGATGATGACCCTGCTCCAGTTCCAGGTCCTCCTGGTGCAATTGAGAAGAAATATTCTGATGCTCTCAAGATGGCCATGAAGTTTTTCGATGTGCAGAAGTGTAATCTTCTCCATCTTATTTCTCATTTTAATATCTTCTGTTTCTACGACATGTTTCTTTTTTGCAATGTCTTGTTTGTTAGTTGTATGAATTTATGTACATATGTGTCTATTTGCAGCTGGAAAGTTGGTAGATAATCAAATATCTTGGAGAGGGGATTCGGGGTTGAAAGATGGAAGTGACGCGAACTTGGATCTGTCTAAGGGAATGTATGATGCTGGGGATCATATGAAGTTTGGTTTTCCAATGGCTTTTACTGCGACAGTCTTATCTTGGTCTATTCTTGAGTATGGAGATCAGATGGATGCAGTTGGTCAATTGGATTCTGCTAAAGATTCTTTGAAGTGGATTACCGACTATCTTATCAATGCTCATCCTTCAGAAAATGTGCTTTACATTCAGGTAACCGTTTATTTACTCATAGTATTGTTCCTGTGTTCTTGCATCTCTATATGACCTGAATTGTAGAACCCGTCAGTTTCGAAACAAGAAGAAGTTGTTGTATGTCGGTTATGTAATCCTTCAAAACTAAATCTATATACTGGGTAACTACTAAAAAAACTTCAATTTATTTTTTGGGATGCTCATGCTTAGATCATTGAGCCTGGATCGCTCACTTTTATGGGGACATTTGACCCACCGAGTGGAGTTAACATGTTGGAGTTGTATTTAGGGTGTAGAGTTGTAAGATCGAGTTCTTCGATAGTTATGAAAAGTAGATTAAAAAAGTAAAATGAAGTTACTTGATAAATGTGAAAAGCAGAGATAGAGAAGAGGATTGAATTCCTTGACAAATGTAAAAAAATATAAAAGAAGATGAAATTACTCGACAAATGTGTAAATTTAATAGTGTTTACTATTGAAGTCAAGTTGTTTTTCAACTACTTATGAAATTGGTGGGCCGAATGACCCCTTTATTGGTTTATTCTATATCACAGATTAATGTAAGGTATTACTGGCTTTCTTTGTAGATTCAAATTGGTCTCAACTGTTTTGTAAGTTATGCGGTAGAATACGTCTCATATAATCTTTTAGTGATACTTTGCAGGTTGGAGATCCTGAGGTAGATCATGCCTGTTGGGATAGGCCTGAAACCATGGTAGAGAAAAGGCCACTCATACAGATCAATGCCTCTGTTCCAGGGACAGAAGTAGCAGCAGAAACTGCTGCAGCTATGGCTTCAGCATCTCTTGTATACAAGAAATCTGATCCCTCCTATTCAAGCAAGCTTCTTAAACATGCTGAAGAATTGTTTTCCTTTGCTGATGAGAATCGAGGTATATACAGTATTAATATTCCTGAAGTTCAGACCTACTACAATTCATCGGGATATGGAGATGAGCTTCTATGGGCTGCTAGCTGGCTGTACCATGCAACACAGGATGAATCCTACCTTGATTATGTAGCTGGAAGCAATGGGAAACTGTATGCAAAATGGGGAAGTCCTACCTGGTTTAGTTGGGACGACAAGCATGCAGCAACTCAAGTACGTATCTCTTTTAACTAGGTGATAGATTCCTATCAATGTGGTAAAATGTAGAATTTTATTAATGTCAGCTGTCGCTAAAGAAATCAGCTGAAGTTTTAGCTGAACAAGAACATTTTACTTCAACATTCTTTTAATTTGTTATGTTTCTCCTTAAGCCATTGTGTAGCTCTATACGGTCTACAGCCTACATGAACATCAGTTCTCATTGAATCTAGAAGCTAATAGTGAGGAGTTGTAACAAAGGGGAATAAGAATGTCTGAAGTCTTGATTGAACCAATTCATGTTATGAGAGCTAAACTTTGGATCAATATTTCAATCTAAACTCATCTCCTGTAGGATTAGACATAGCATGACTTCATTTTCCTGCATTAAACATATTATCTCGATACCATTCATCTCATACACAATAGAACATTTCTTTCCCCTTGTAACTCTGTCTGGAATCAAGACAAGCAAATTACCTTAAAGCTACAAATGATCTAACATTCTTAATATTGTATAAAATGTTTTTGAAAGTAAAATTGAGATGAAAATAGAGTAAGCATTCTATGATCATAGACTCATGAAAGGGATGTTAAAATTTTATGTGGTTACGTCTGGGATTAGCTTTGAAATCATTAGAAATCCAAGCACGAGTAAGGAAAATCTTTTACACAAGAAAAAGGTTGTAAACCACTTTTGATTTCTGACTCCTATTTTTAGTGATAAACAAGTGAATTTGTTTCAAACTTATTGCAGATTAAATTATTTCAAATCCTTCTATTTTAAATGATAAACAAATGAACATTGACATCTTAACCTTGTCACTAACTCTGCAATGCCCATGCAGGTTTTACTGTCTCGGTTAAACTTCTTTGGCTCCGAAAGCGCTTCAAAGTCGGTTCAAGTTTACAAGAAAACAGCCGAAGCTGTCATGTGTGGTCTAATCCCAGAGTCTCCAACAGCAACAAGCAGCAGAACAGACAGTAAGTAGTTTATTTGAGGTTGAAATATCATTTTGGTCACTATAGTTTCAAATATCTAATTTTAGTCCATCTACTCATCCTCAATAATTCTTAAAATTAGTCCCTACAGTTTGTTGATTTTTTCCAAAAAGATTTTTTGTCATCTATCGCATGAAATTTTTTATTCTTACCTGACCATTTTTTATTTAAACAAAAAAGGATACCTTTGGAGGACTACATTGAAGATTTATTGATGGTACATGGACCAAAATTGCATTTTAACCTTTATTTCAACTATTCAGAAACTACATAAGATGGAATTTCTTGTCATCTCTAGGATCAGGAATATTGCATTGTCGTTTAGAACTTCTTAACTTATTTAACGATGTTTTTGGGACAGATGGTCTCATATGGGTCAGCGAATGGAATTCTCTGCAGCATCCTGTGTCTTCAGCATTCTTAGCTTCTCTTTACAGCGATTACATGCTTACCTCCAGAACTGCAAAATTTTCTTGCAATGGAGACACCTACACACCAGCAGACCTACGGAAATTTGCCAAATCTCAGGTTAGCAAACAGTTTATCTACCTCAACAGCATATACTTTTCTCTTGAGAAAGTGCTAATGATTTTAAGTTGAACGCTTCTCTTTCCAGGCTGATTATGTGTTGGGCAACAATCCTTTGAAAATGAGCTATCTCGTGGGATATGGTGACAAATTCCCACAATATGTCCACCACAGAGGTGCATCAATCCCAGCTGATGCCAAAACCAAGTGTAAAGATGGCTTTAAGTGGCTCGACTCCACTGAACCCAACCCAAATATAGCTTATGGAGCTCTTGTTGGTGGTCCTTTCCTTAACGACACCTATATAGATTCACGAAACAATTCAATGCAAGGGGAACCGAGCACATATAACAGTGCTCTTGTGGTTGGCCTGCTATCGAGTTTAGTTACCACCTCTTCAGCAGTCCAGTCCTTCACCTAATGCAACCCATACACGCATATAACCATATCATATTATCTAAAATTGTGATTGTATTATATCTTGTAGCTTACCACCTATTTGTACGAGAAAATATTAGTGCTATGATGAGTTTACTTCTTCCAAATGAGTGAGCGTTTGCCAATCAATCCTCTAACAAAAACCCAATCCGCCACACAGTGAAATTAAATTAACCATGTATTATCTGAAGTGAGCCATTGTTTGAACAATTATGGTATGTAATTCTTTTATTTGAGTGAGATATATGTTGTAATTTGTTGTTGCCATGTTCATTCAGTTCATTGTTGATGACAAGATGAGTTGGTTTGAAAGGTCCATTGGCCAATTGAAAAAAAGTCGATGCTTCTGTTCCACGTTAACAAAAAGGAAGAAGATGACGTGACATTGCACAGAAATTGGAGGGTAAGTTTGATGT

Coding sequence (CDS)

ATGGGGGAGAAATCGAGGTCTAGAGGCTGTTGTGGATGGTTTCTTGTTTTGGTGGTTCTTGCTTTGATTGCTGGTGCTATAGTATTTGCAGTCAAGAAGAAGTCGAGTCACTCTGATGATGACCCTGCTCCAGTTCCAGGTCCTCCTGGTGCAATTGAGAAGAAATATTCTGATGCTCTCAAGATGGCCATGAAGTTTTTCGATGTGCAGAAGTCTGGAAAGTTGGTAGATAATCAAATATCTTGGAGAGGGGATTCGGGGTTGAAAGATGGAAGTGACGCGAACTTGGATCTGTCTAAGGGAATGTATGATGCTGGGGATCATATGAAGTTTGGTTTTCCAATGGCTTTTACTGCGACAGTCTTATCTTGGTCTATTCTTGAGTATGGAGATCAGATGGATGCAGTTGGTCAATTGGATTCTGCTAAAGATTCTTTGAAGTGGATTACCGACTATCTTATCAATGCTCATCCTTCAGAAAATGTGCTTTACATTCAGGTTGGAGATCCTGAGGTAGATCATGCCTGTTGGGATAGGCCTGAAACCATGGTAGAGAAAAGGCCACTCATACAGATCAATGCCTCTGTTCCAGGGACAGAAGTAGCAGCAGAAACTGCTGCAGCTATGGCTTCAGCATCTCTTGTATACAAGAAATCTGATCCCTCCTATTCAAGCAAGCTTCTTAAACATGCTGAAGAATTGTTTTCCTTTGCTGATGAGAATCGAGGTATATACAGTATTAATATTCCTGAAGTTCAGACCTACTACAATTCATCGGGATATGGAGATGAGCTTCTATGGGCTGCTAGCTGGCTGTACCATGCAACACAGGATGAATCCTACCTTGATTATGTAGCTGGAAGCAATGGGAAACTGTATGCAAAATGGGGAAGTCCTACCTGGTTTAGTTGGGACGACAAGCATGCAGCAACTCAAGTTTTACTGTCTCGGTTAAACTTCTTTGGCTCCGAAAGCGCTTCAAAGTCGGTTCAAGTTTACAAGAAAACAGCCGAAGCTGTCATGTGTGGTCTAATCCCAGAGTCTCCAACAGCAACAAGCAGCAGAACAGACAATGGTCTCATATGGGTCAGCGAATGGAATTCTCTGCAGCATCCTGTGTCTTCAGCATTCTTAGCTTCTCTTTACAGCGATTACATGCTTACCTCCAGAACTGCAAAATTTTCTTGCAATGGAGACACCTACACACCAGCAGACCTACGGAAATTTGCCAAATCTCAGGCTGATTATGTGTTGGGCAACAATCCTTTGAAAATGAGCTATCTCGTGGGATATGGTGACAAATTCCCACAATATGTCCACCACAGAGGTGCATCAATCCCAGCTGATGCCAAAACCAAGTGTAAAGATGGCTTTAAGTGGCTCGACTCCACTGAACCCAACCCAAATATAGCTTATGGAGCTCTTGTTGGTGGTCCTTTCCTTAACGACACCTATATAGATTCACGAAACAATTCAATGCAAGGGGAACCGAGCACATATAACAGTGCTCTTGTGGTTGGCCTGCTATCGAGTTTAGTTACCACCTCTTCAGCAGTCCAGTCCTTCACCTAA

Protein sequence

MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDALKMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTATVLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRPETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADENRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPTWFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGLIWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGNNPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPFLNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT
Homology
BLAST of Cucsat.G12896 vs. ExPASy Swiss-Prot
Match: Q8LCP6 (Endoglucanase 10 OS=Arabidopsis thaliana OX=3702 GN=At1g75680 PE=2 SV=2)

HSP 1 Score: 786.9 bits (2031), Expect = 1.4e-226
Identity = 379/524 (72.33%), Postives = 450/524 (85.88%), Query Frame = 0

Query: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVK--KKSSHSDDDPAPVPGPPGAIEKKYSD 60
           M EKS+SRG CGWF+ ++VLA +  A+V+ VK   K S  DD   PVPGPPGAI+KKY+D
Sbjct: 1   MEEKSKSRGWCGWFIAIIVLASVILAVVYTVKLRTKKSGDDDGGGPVPGPPGAIDKKYAD 60

Query: 61  ALKMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFT 120
           ALK+A++FFD+QKSGKL +N+I WRGDSGLKDGS+ NLDLSKG+YDAGDH+KFGFPMAFT
Sbjct: 61  ALKLALQFFDIQKSGKLENNKIPWRGDSGLKDGSEDNLDLSKGLYDAGDHIKFGFPMAFT 120

Query: 121 ATVLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWD 180
           ATVLSWSILEYGDQM+AV QLD AKDSL+WITDYLI AHPS+NVLYIQVGDP+VDH CW+
Sbjct: 121 ATVLSWSILEYGDQMNAVNQLDPAKDSLRWITDYLIKAHPSDNVLYIQVGDPKVDHPCWE 180

Query: 181 RPETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFA 240
           RPE M EKRPL +I+   PGTEVAAETAAAMASASLV+K SDP+YS+ LLKHA++LF+FA
Sbjct: 181 RPEDMKEKRPLTKIDVDTPGTEVAAETAAAMASASLVFKDSDPTYSATLLKHAKQLFNFA 240

Query: 241 DENRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGS 300
           D  RG YS+NIPEVQ +YNS+GYGDELLWAASWLYHAT+D++YLDYV+ ++GK +A +G+
Sbjct: 241 DTKRGSYSVNIPEVQKFYNSTGYGDELLWAASWLYHATEDKTYLDYVS-NHGKEFASFGN 300

Query: 301 PTWFSWDDKHAATQVLLSRLNFFGSE-SASKSVQVYKKTAEAVMCGLIPESPTATSSRTD 360
           PTWFSWD+K A TQVLLSRL FF  + S SK +  Y+ TA+AVMCGL+P+SPT+T+SRT+
Sbjct: 301 PTWFSWDNKLAGTQVLLSRLLFFKKDLSGSKGLGNYRNTAKAVMCGLLPKSPTSTASRTN 360

Query: 361 NGLIWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYV 420
            GLIWVSEWNS+Q  VSSAFLASL+SDYMLTSR  K SC+G  +   +LR FAKSQADY+
Sbjct: 361 GGLIWVSEWNSMQQSVSSAFLASLFSDYMLTSRIHKISCDGKIFKATELRDFAKSQADYM 420

Query: 421 LGNNPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVG 480
           LG NPL  S++VGYGDK+PQ+VHHRGASIPADA T C DGFKW +ST+PNPNIAYGALVG
Sbjct: 421 LGKNPLGTSFVVGYGDKYPQFVHHRGASIPADATTGCLDGFKWFNSTKPNPNIAYGALVG 480

Query: 481 GPFLNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQS 522
           GPF N+T+ DSR N MQ EP+TYN+AL+VGLLSSLVTTSS +QS
Sbjct: 481 GPFFNETFTDSRENPMQNEPTTYNNALLVGLLSSLVTTSSTLQS 523

BLAST of Cucsat.G12896 vs. ExPASy Swiss-Prot
Match: Q9FXI9 (Endoglucanase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g19940 PE=2 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 5.3e-223
Identity = 376/524 (71.76%), Postives = 444/524 (84.73%), Query Frame = 0

Query: 1   MGEKSRSR-GCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDA 60
           M  K RSR  CC  F+ +++L  I  A++F ++ +S+HSDDD        G+  K Y++A
Sbjct: 1   MVAKPRSRCCCCSVFIGVIILIAIIIAVIFTIRHRSNHSDDD--------GSNVKNYANA 60

Query: 61  LKMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTA 120
           LK+AM+FFD+QKSGKL +N+ISWRGDSGLKDGS+A++DLSKG+YDAGDHMKFGFPMAFTA
Sbjct: 61  LKIAMQFFDIQKSGKLENNEISWRGDSGLKDGSEASIDLSKGLYDAGDHMKFGFPMAFTA 120

Query: 121 TVLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDR 180
           TVLSWSILEYGDQM ++  LD AKDSLKW TD+LINAHPS NVLYIQVGDP  DH CWDR
Sbjct: 121 TVLSWSILEYGDQMASLNLLDHAKDSLKWTTDFLINAHPSPNVLYIQVGDPVTDHKCWDR 180

Query: 181 PETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFAD 240
           PETM  KR L +I+   PGTEVAAETAAAMA+ASLV+K+SD  YSS LLKHA++LF FAD
Sbjct: 181 PETMTRKRTLTKIDTKTPGTEVAAETAAAMAAASLVFKESDTKYSSTLLKHAKQLFDFAD 240

Query: 241 ENRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSP 300
            NRG YS+NIPEVQ+YYNS+GYGDELLWAASWLYHAT+D++YLD+V+  NG+ +  +GSP
Sbjct: 241 NNRGSYSVNIPEVQSYYNSTGYGDELLWAASWLYHATEDQTYLDFVS-ENGEEFGNFGSP 300

Query: 301 TWFSWDDKHAATQVLLSRLNFFGSE-SASKSVQVYKKTAEAVMCGLIPESPTATSSRTDN 360
           +WFSWD+K   T +LLSRL FF    S SK +Q +K+TAEAVMCGLIP SPTATSSRTD 
Sbjct: 301 SWFSWDNKLPGTHILLSRLTFFKKGLSGSKGLQGFKETAEAVMCGLIPSSPTATSSRTDG 360

Query: 361 GLIWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVL 420
           GLIWVSEWN+LQHPVSSAFLA+LYSDYMLTS   + SC+  ++ P+DLRKFA+SQADY+L
Sbjct: 361 GLIWVSEWNALQHPVSSAFLATLYSDYMLTSGVKELSCSDQSFKPSDLRKFARSQADYML 420

Query: 421 GNNPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGG 480
           G NP KMSYLVGYG+K+P++VHHRGASIPADA T CKDGFKWL+S EPNPN+AYGALVGG
Sbjct: 421 GKNPEKMSYLVGYGEKYPEFVHHRGASIPADATTGCKDGFKWLNSDEPNPNVAYGALVGG 480

Query: 481 PFLNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSF 523
           PFLNDT+ID+RNNSMQ EPSTYNSALVVGLLSSLVTTSS+V+SF
Sbjct: 481 PFLNDTFIDARNNSMQNEPSTYNSALVVGLLSSLVTTSSSVESF 515

BLAST of Cucsat.G12896 vs. ExPASy Swiss-Prot
Match: Q69SG5 (Endoglucanase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0533900 PE=2 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 1.7e-208
Identity = 348/527 (66.03%), Postives = 423/527 (80.27%), Query Frame = 0

Query: 5   SRSRGCCGWFLVLVVLALIAGAIVFAVKKK----SSHSDDDPAPVPGPPGAIEKKYSDAL 64
           S+++GCCGW +V +V +L+A A V A+ KK     S     P PVPGPPGAI+ KY DAL
Sbjct: 3   SKTKGCCGWLIVALVASLVATAAVVAIMKKKVGGGSGRKLKPLPVPGPPGAIDSKYGDAL 62

Query: 65  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 124
            +A++FF VQK+GKL +NQI WRGDS L DG  A LDLSKGMYDAGDH+KF FPMAFTAT
Sbjct: 63  GVALQFFQVQKAGKLENNQIPWRGDSALDDGKPAGLDLSKGMYDAGDHIKFSFPMAFTAT 122

Query: 125 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 184
           VLSWSILEYGDQM A  QLD A D+L+WITD+L+NAHPS+NV YIQVGDP++DH CW+RP
Sbjct: 123 VLSWSILEYGDQMSATKQLDPALDALRWITDFLVNAHPSDNVFYIQVGDPDLDHNCWERP 182

Query: 185 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 244
           ETM EKRPL QIN   PG++VAAE AAAMASAS+V+K  D +YS  LL+HA++LF+FAD 
Sbjct: 183 ETMSEKRPLTQINTKSPGSDVAAEAAAAMASASIVFKSRDTTYSDSLLQHAQKLFTFADT 242

Query: 245 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 304
            +G+ S   P++Q YYNS+GY DELLWAASWLYHAT D++YL YV   NGK +A WG PT
Sbjct: 243 YKGLASDTYPKLQNYYNSTGYQDELLWAASWLYHATGDQTYLSYVTVENGKAFADWGRPT 302

Query: 305 WFSWDDKHAATQVLLSRLNFFGSESASKS----VQVYKKTAEAVMCGLIPESPTATSSRT 364
           WFSWDDK A TQVLLSRLNFFGS+  S +    +++Y+ TAEAV+CGL+P+SP+AT+SRT
Sbjct: 303 WFSWDDKLAGTQVLLSRLNFFGSKQTSNAENMGLKMYRDTAEAVICGLLPDSPSATASRT 362

Query: 365 DNGLIWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADY 424
             GL+W+S WNSLQH  ++AFLA +YSDYMLTS+TA   C+G  Y+P D+R FA SQA+Y
Sbjct: 363 GGGLVWISGWNSLQHATNAAFLAVVYSDYMLTSQTAAVQCSGKYYSPTDIRNFAISQANY 422

Query: 425 VLGNNPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALV 484
           +LG+NP+K+SYLVGYG  +PQ VHHRGASIPADAKT CK GF++L ST PNPN+A GALV
Sbjct: 423 ILGDNPMKLSYLVGYGSSYPQQVHHRGASIPADAKTGCK-GFQYLHSTSPNPNVAMGALV 482

Query: 485 GGPFLNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 524
           GGPF NDT++DSR+N++Q E STYNS  +VGLLS LVTTSS  QSFT
Sbjct: 483 GGPFQNDTFVDSRDNAVQTESSTYNSGTLVGLLSGLVTTSSVAQSFT 528

BLAST of Cucsat.G12896 vs. ExPASy Swiss-Prot
Match: Q5Z9P8 (Endoglucanase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0715300 PE=2 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 4.7e-187
Identity = 321/518 (61.97%), Postives = 408/518 (78.76%), Query Frame = 0

Query: 10  CCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDALKMAMKFFDV 69
           CC W LVL+++AL   A V  V+ K+    +   P PG PGA++ KY+DAL +A++FF V
Sbjct: 7   CCCWLLVLMLMALAITAAVVFVRYKNG---EGVFPFPGVPGAVDHKYADALAVALQFFQV 66

Query: 70  QKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTATVLSWSILEY 129
           QKSGKLV+N I WRGDS L DG +A +DLSKGMYDAGDHMKFGFPMAFTAT+LSWS+LEY
Sbjct: 67  QKSGKLVNNTIHWRGDSALDDGKEAGIDLSKGMYDAGDHMKFGFPMAFTATMLSWSVLEY 126

Query: 130 GDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRPETMVEKRPL 189
           GD M A  Q DSA D+L WI DYL+NAHPS++VLYIQVGDP+ DH CW+RPE M EKRPL
Sbjct: 127 GDAMRAADQRDSAIDALNWIMDYLVNAHPSDDVLYIQVGDPKADHKCWERPEKMKEKRPL 186

Query: 190 IQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADENRGIYSINI 249
            +I    PG++VAAETAAAMA+ASLVYK  + +YSS LL H E LF+FAD++RG Y+   
Sbjct: 187 TKITPKSPGSDVAAETAAAMAAASLVYKTINKTYSSSLLDHGERLFAFADKHRGSYTRTF 246

Query: 250 PEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPTWFSWDDKHA 309
           PE+  +YNS+ Y DELLWAASWLYHAT + SYL Y  G N K +A  G+P +FSWDDK A
Sbjct: 247 PELSAFYNSTTYQDELLWAASWLYHATGNHSYLAYATGKN-KDFADLGNPRYFSWDDKRA 306

Query: 310 ATQVLLSRLNFFGSESASKS----VQVYKKTAEAVMCGLIPESPTATSSRTDNGLIWVSE 369
            T+VLLSR++FF S+ +  +    + +YK+TA+AVMC L+P+S TA + RT+ GL++V+E
Sbjct: 307 GTEVLLSRVSFFASQGSDVAQDDVLGMYKQTADAVMCILLPDSETA-AFRTEGGLLYVAE 366

Query: 370 WNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGNNPLKM 429
           WNSLQHPV+SAFLA++YSDYM +S   + SC+G  ++PADLRKFAKSQADY+LG+NP+K+
Sbjct: 367 WNSLQHPVASAFLAAVYSDYMQSSGKTELSCSGQGFSPADLRKFAKSQADYLLGSNPMKI 426

Query: 430 SYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPFLNDTY 489
           SYLVGYGD++P+ VHHRGASIP D  T C DG KWL++++PNPN+A GALVGGP+ ND++
Sbjct: 427 SYLVGYGDRYPEKVHHRGASIPEDVDTGC-DGHKWLETSKPNPNVATGALVGGPYKNDSF 486

Query: 490 IDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 524
           +D R+N MQ E +TYNSALV GLLS+LV+TSS  +S +
Sbjct: 487 VDERDNVMQNEATTYNSALVAGLLSALVSTSSLARSLS 518

BLAST of Cucsat.G12896 vs. ExPASy Swiss-Prot
Match: Q8L7I0 (Endoglucanase 19 OS=Arabidopsis thaliana OX=3702 GN=At4g11050 PE=2 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.7e-118
Identity = 220/470 (46.81%), Postives = 310/470 (65.96%), Query Frame = 0

Query: 56  YSDALKMAMKFFDVQKSGKLVDNQ-ISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFP 115
           Y  AL  ++ FF+ Q+SG L  NQ +SWR  SGL DG  + +DL  G YDAGD++KFG P
Sbjct: 27  YKQALSKSILFFEAQRSGHLPPNQRVSWRSHSGLYDGKSSGVDLVGGYYDAGDNVKFGLP 86

Query: 116 MAFTATVLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDH 175
           MAFT T + WSI+EYG Q+++ G+L  A D++KW TDY I AHP  NVLY +VGD + DH
Sbjct: 87  MAFTVTTMCWSIIEYGGQLESNGELGHAIDAVKWGTDYFIKAHPEPNVLYGEVGDGKSDH 146

Query: 176 ACWDRPETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEEL 235
            CW RPE M   R   +I+ + PG+++A ETAAAMA+AS+V+++SDPSYS++LL+HA +L
Sbjct: 147 YCWQRPEEMTTDRRAYKIDRNNPGSDLAGETAAAMAAASIVFRRSDPSYSAELLRHAHQL 206

Query: 236 FSFADENRGIYSINIPEVQTYYNS-SGYGDELLWAASWLYHATQDESYLDYVAGSNGKLY 295
           F FAD+ RG Y  +I   Q YY S SGY DELLWAA+WLY AT D+ YLDY+  +   + 
Sbjct: 207 FEFADKYRGKYDSSITVAQKYYRSVSGYNDELLWAAAWLYQATNDKYYLDYLGKNGDSMG 266

Query: 296 AKWGSPTWFSWDDKHAATQVLLSRLNFFGSESASKSV-QVYKKTAEAVMCGLIPESPTAT 355
               S T F WD K+A  Q L++++   G      +V + Y++ AE  MC L+ +S T  
Sbjct: 267 GTGWSMTEFGWDVKYAGVQTLVAKVLMQGKGGEHTAVFERYQQKAEQFMCSLLGKS-TKN 326

Query: 356 SSRTDNGLIWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKS 415
             +T  GLI+   WN++Q   S++FLA++YSDY+  S+     C+    +P+ L +F+KS
Sbjct: 327 IKKTPGGLIFRQSWNNMQFVTSASFLATVYSDYLSYSK-RDLLCSQGNISPSQLLEFSKS 386

Query: 416 QADYVLGNNPLKMSYLVGYGDKFPQYVHHRGASIPA---DAK-TKCKDGF-KWLDSTEPN 475
           Q DY+LG+NP   SY+VGYG+ +P+ VHHRG+SI +   D K   C+ G+  W      +
Sbjct: 387 QVDYILGDNPRATSYMVGYGENYPRQVHHRGSSIVSFNVDQKFVTCRGGYATWFSRKGSD 446

Query: 476 PNIAYGALVGGPFLNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSS 518
           PN+  GALVGGP   D + D R+N  Q EP+TYN+A ++G+L+ L++ S+
Sbjct: 447 PNVLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPLLGVLARLISGST 494

BLAST of Cucsat.G12896 vs. NCBI nr
Match: XP_004136389.1 (endoglucanase 10 [Cucumis sativus] >KGN60025.1 hypothetical protein Csa_001861 [Cucumis sativus])

HSP 1 Score: 1058 bits (2735), Expect = 0.0
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 0

Query: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60
           MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL
Sbjct: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60

Query: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120
           KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT
Sbjct: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120

Query: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180
           VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP
Sbjct: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180

Query: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240
           ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE
Sbjct: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240

Query: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300
           NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT
Sbjct: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300

Query: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360
           WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL
Sbjct: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360

Query: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420
           IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN
Sbjct: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420

Query: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480
           NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF
Sbjct: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480

Query: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523
           LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT
Sbjct: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523

BLAST of Cucsat.G12896 vs. NCBI nr
Match: XP_008466493.1 (PREDICTED: endoglucanase 10 [Cucumis melo] >KAA0063276.1 endoglucanase 10 [Cucumis melo var. makuwa] >TYK31499.1 endoglucanase 10 [Cucumis melo var. makuwa])

HSP 1 Score: 1042 bits (2694), Expect = 0.0
Identity = 511/523 (97.71%), Postives = 520/523 (99.43%), Query Frame = 0

Query: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60
           MGEKSRSRGCCGWFLVLV+L LIAGAIVFAVKKKSSHSDDDPAPVPGPPGAI+KKYSDAL
Sbjct: 1   MGEKSRSRGCCGWFLVLVILGLIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIQKKYSDAL 60

Query: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120
           KMA++FFDVQKSGKLVDN+ISWRGDSGLKDGS+ANLDLSKGMYDAGDHMKFGFPMAFTAT
Sbjct: 61  KMALRFFDVQKSGKLVDNKISWRGDSGLKDGSEANLDLSKGMYDAGDHMKFGFPMAFTAT 120

Query: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180
           VLSWSILEYGDQMDAVGQLD AKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP
Sbjct: 121 VLSWSILEYGDQMDAVGQLDPAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180

Query: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240
           ETM EKRPLIQINASVPGT+VAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE
Sbjct: 181 ETMAEKRPLIQINASVPGTDVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240

Query: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300
           NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDE+YLDYVAGSNGKLYAKWGSPT
Sbjct: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDETYLDYVAGSNGKLYAKWGSPT 300

Query: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360
           WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL
Sbjct: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360

Query: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420
           IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGD+YTPADLRKFAKSQADYVLGN
Sbjct: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDSYTPADLRKFAKSQADYVLGN 420

Query: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480
           NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF
Sbjct: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480

Query: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523
           LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT
Sbjct: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523

BLAST of Cucsat.G12896 vs. NCBI nr
Match: XP_038898468.1 (endoglucanase 10-like [Benincasa hispida])

HSP 1 Score: 1023 bits (2645), Expect = 0.0
Identity = 499/523 (95.41%), Postives = 514/523 (98.28%), Query Frame = 0

Query: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60
           MGEKSRSRGCCGWFLVLV+L LIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL
Sbjct: 1   MGEKSRSRGCCGWFLVLVILGLIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60

Query: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120
           KMAM+FFDVQKSGKLVDN+ISWRGDSGLKDGS+ANLDLSKGMYDAGDHMKFGFPMAFTAT
Sbjct: 61  KMAMRFFDVQKSGKLVDNKISWRGDSGLKDGSEANLDLSKGMYDAGDHMKFGFPMAFTAT 120

Query: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180
           VLSW+ILEYGDQMDAVGQLD AKDSLKWITDYLINAHP+ENVLYIQVGDP+VDHACWDRP
Sbjct: 121 VLSWAILEYGDQMDAVGQLDPAKDSLKWITDYLINAHPAENVLYIQVGDPKVDHACWDRP 180

Query: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240
           ETM EKRPL+QINASVPGT+VAAETAAAMASASLVYKKSDPSYS+KLLKHAEELFSFAD 
Sbjct: 181 ETMAEKRPLVQINASVPGTDVAAETAAAMASASLVYKKSDPSYSNKLLKHAEELFSFADG 240

Query: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300
           NRG+YSINIPEVQTYYNSSGYGDELLWAASWLYHAT+DE+YLDYVAGSNGKLYAKWGSPT
Sbjct: 241 NRGLYSINIPEVQTYYNSSGYGDELLWAASWLYHATEDEAYLDYVAGSNGKLYAKWGSPT 300

Query: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360
           WFSWDDKHAATQVLLSRLNFFGSE  SKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL
Sbjct: 301 WFSWDDKHAATQVLLSRLNFFGSEGISKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360

Query: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420
           IWVSEWNSLQHPVSSAFLASLY DYMLTSRTAK SC+GD+YTPADLRKFAKSQADYVLGN
Sbjct: 361 IWVSEWNSLQHPVSSAFLASLYGDYMLTSRTAKLSCSGDSYTPADLRKFAKSQADYVLGN 420

Query: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480
           NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKC DGFKWLDSTEPNPNIAYGALVGGPF
Sbjct: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCNDGFKWLDSTEPNPNIAYGALVGGPF 480

Query: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523
           LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT
Sbjct: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523

BLAST of Cucsat.G12896 vs. NCBI nr
Match: XP_022141104.1 (endoglucanase 10-like [Momordica charantia])

HSP 1 Score: 980 bits (2533), Expect = 0.0
Identity = 475/522 (91.00%), Postives = 504/522 (96.55%), Query Frame = 0

Query: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60
           MGEKSRSRGCCGWFLVLV+L L+AGAIVFAVKKK SHSDD+ APVPGPPGAIEKKYSDAL
Sbjct: 1   MGEKSRSRGCCGWFLVLVILGLVAGAIVFAVKKKMSHSDDEAAPVPGPPGAIEKKYSDAL 60

Query: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120
           KMAMKFFDVQKSG+LVDN ISWRGDSGLKDGS+A LDLSKGMYDAGDHMKFGFPMAFTAT
Sbjct: 61  KMAMKFFDVQKSGELVDNGISWRGDSGLKDGSEAKLDLSKGMYDAGDHMKFGFPMAFTAT 120

Query: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180
           VLSWSILEYGDQMDAVGQLD AKDSLKWITDYLINAHPS+NVLYIQVGDP+VDH CWDRP
Sbjct: 121 VLSWSILEYGDQMDAVGQLDPAKDSLKWITDYLINAHPSKNVLYIQVGDPKVDHGCWDRP 180

Query: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240
           ETM EKRPL QINASVPGTEVAAETAAAMA+ASLVYK+++PSYSS LLKHA+ELFSFADE
Sbjct: 181 ETMTEKRPLRQINASVPGTEVAAETAAAMAAASLVYKETNPSYSSTLLKHAQELFSFADE 240

Query: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300
           NRG+YSI+IPEVQTYYNSSGYGDELLWAASWLYHAT+DES+LDYV G NGKLYAKWGSPT
Sbjct: 241 NRGLYSISIPEVQTYYNSSGYGDELLWAASWLYHATEDESFLDYVTGDNGKLYAKWGSPT 300

Query: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360
           WFSWDDKHAATQVLLSRL+FFGS+  SKSVQVYKKTAEAVMCGLIPESPTAT+SRTDNGL
Sbjct: 301 WFSWDDKHAATQVLLSRLSFFGSKDVSKSVQVYKKTAEAVMCGLIPESPTATTSRTDNGL 360

Query: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420
           IWVSEWNSLQHPVSSAFLA+LYSDYMLTSRT K SC+GD++TPADLRKFAKSQADYVLG 
Sbjct: 361 IWVSEWNSLQHPVSSAFLAALYSDYMLTSRTPKLSCSGDSFTPADLRKFAKSQADYVLGE 420

Query: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480
           NPLKMSYLVGYGDKFP+YVHHRGASIPADA+TKCKDGFKWLDST+PNPN+A GALVGGPF
Sbjct: 421 NPLKMSYLVGYGDKFPKYVHHRGASIPADAETKCKDGFKWLDSTKPNPNVASGALVGGPF 480

Query: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSF 522
           LN+TYIDSRNNSMQGEPSTYNSAL+VGLLSSLVTTSSAVQSF
Sbjct: 481 LNETYIDSRNNSMQGEPSTYNSALIVGLLSSLVTTSSAVQSF 522

BLAST of Cucsat.G12896 vs. NCBI nr
Match: XP_023525365.1 (endoglucanase 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 974 bits (2517), Expect = 0.0
Identity = 472/523 (90.25%), Postives = 502/523 (95.98%), Query Frame = 0

Query: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60
           MGEKSRSRG CGWFLVLV+L LIAGAIVFAVKKKSSHSDD+PAPVPGPPGAI KKYSDAL
Sbjct: 1   MGEKSRSRGFCGWFLVLVILGLIAGAIVFAVKKKSSHSDDEPAPVPGPPGAIVKKYSDAL 60

Query: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120
           K+AMKFFDVQKSGKLVDN ISWRGDSGLKDG++A LDLSKGMYDAGDH+KFGFPMAFTAT
Sbjct: 61  KIAMKFFDVQKSGKLVDNTISWRGDSGLKDGTEAKLDLSKGMYDAGDHIKFGFPMAFTAT 120

Query: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180
           VLSWSILEYGDQMD VGQLD AKDSLKWITDYLINAHPSENVLYIQVGDP++DHACWDRP
Sbjct: 121 VLSWSILEYGDQMDVVGQLDPAKDSLKWITDYLINAHPSENVLYIQVGDPDIDHACWDRP 180

Query: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240
           ETM  KRPL  INAS PGT+VAAETAAAMASASLVYKK+DP+YS  LLKHAEELFSFADE
Sbjct: 181 ETMTGKRPLTMINASFPGTDVAAETAAAMASASLVYKKTDPTYSDLLLKHAEELFSFADE 240

Query: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300
           +RG+YS++IP+VQTYYNS+GYGDELLWAASWLYHAT+DE+YL+YV+G NGKLYAKWGSPT
Sbjct: 241 HRGLYSVSIPKVQTYYNSTGYGDELLWAASWLYHATEDETYLNYVSGDNGKLYAKWGSPT 300

Query: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360
           WFSWDDKHAATQVLLSRLNFFGSE  SKSVQVYKKTAEAVMCGLIPESPTATSSRTD+GL
Sbjct: 301 WFSWDDKHAATQVLLSRLNFFGSEGVSKSVQVYKKTAEAVMCGLIPESPTATSSRTDDGL 360

Query: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420
           IWVSEWN+LQHPVSSAFLASLYSDYMLTSRTAKFSC+G+TYTPADLR FAKSQADYVLGN
Sbjct: 361 IWVSEWNALQHPVSSAFLASLYSDYMLTSRTAKFSCSGETYTPADLRNFAKSQADYVLGN 420

Query: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480
           NP+KMSYLVGYGDKFPQYVHHRGASIP DA TKCKDGFKWL ST+PNPNIAYGALVGGPF
Sbjct: 421 NPMKMSYLVGYGDKFPQYVHHRGASIPTDATTKCKDGFKWLLSTKPNPNIAYGALVGGPF 480

Query: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523
           LN++YIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSA+QSFT
Sbjct: 481 LNESYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSALQSFT 523

BLAST of Cucsat.G12896 vs. ExPASy TrEMBL
Match: A0A0A0LDK2 (Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_3G872070 PE=3 SV=1)

HSP 1 Score: 1058 bits (2735), Expect = 0.0
Identity = 523/523 (100.00%), Postives = 523/523 (100.00%), Query Frame = 0

Query: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60
           MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL
Sbjct: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60

Query: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120
           KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT
Sbjct: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120

Query: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180
           VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP
Sbjct: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180

Query: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240
           ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE
Sbjct: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240

Query: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300
           NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT
Sbjct: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300

Query: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360
           WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL
Sbjct: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360

Query: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420
           IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN
Sbjct: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420

Query: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480
           NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF
Sbjct: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480

Query: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523
           LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT
Sbjct: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523

BLAST of Cucsat.G12896 vs. ExPASy TrEMBL
Match: A0A5D3E6M4 (Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G008150 PE=3 SV=1)

HSP 1 Score: 1042 bits (2694), Expect = 0.0
Identity = 511/523 (97.71%), Postives = 520/523 (99.43%), Query Frame = 0

Query: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60
           MGEKSRSRGCCGWFLVLV+L LIAGAIVFAVKKKSSHSDDDPAPVPGPPGAI+KKYSDAL
Sbjct: 1   MGEKSRSRGCCGWFLVLVILGLIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIQKKYSDAL 60

Query: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120
           KMA++FFDVQKSGKLVDN+ISWRGDSGLKDGS+ANLDLSKGMYDAGDHMKFGFPMAFTAT
Sbjct: 61  KMALRFFDVQKSGKLVDNKISWRGDSGLKDGSEANLDLSKGMYDAGDHMKFGFPMAFTAT 120

Query: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180
           VLSWSILEYGDQMDAVGQLD AKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP
Sbjct: 121 VLSWSILEYGDQMDAVGQLDPAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180

Query: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240
           ETM EKRPLIQINASVPGT+VAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE
Sbjct: 181 ETMAEKRPLIQINASVPGTDVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240

Query: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300
           NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDE+YLDYVAGSNGKLYAKWGSPT
Sbjct: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDETYLDYVAGSNGKLYAKWGSPT 300

Query: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360
           WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL
Sbjct: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360

Query: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420
           IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGD+YTPADLRKFAKSQADYVLGN
Sbjct: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDSYTPADLRKFAKSQADYVLGN 420

Query: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480
           NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF
Sbjct: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480

Query: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523
           LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT
Sbjct: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523

BLAST of Cucsat.G12896 vs. ExPASy TrEMBL
Match: A0A1S3CRJ7 (Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103503885 PE=3 SV=1)

HSP 1 Score: 1042 bits (2694), Expect = 0.0
Identity = 511/523 (97.71%), Postives = 520/523 (99.43%), Query Frame = 0

Query: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60
           MGEKSRSRGCCGWFLVLV+L LIAGAIVFAVKKKSSHSDDDPAPVPGPPGAI+KKYSDAL
Sbjct: 1   MGEKSRSRGCCGWFLVLVILGLIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIQKKYSDAL 60

Query: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120
           KMA++FFDVQKSGKLVDN+ISWRGDSGLKDGS+ANLDLSKGMYDAGDHMKFGFPMAFTAT
Sbjct: 61  KMALRFFDVQKSGKLVDNKISWRGDSGLKDGSEANLDLSKGMYDAGDHMKFGFPMAFTAT 120

Query: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180
           VLSWSILEYGDQMDAVGQLD AKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP
Sbjct: 121 VLSWSILEYGDQMDAVGQLDPAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180

Query: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240
           ETM EKRPLIQINASVPGT+VAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE
Sbjct: 181 ETMAEKRPLIQINASVPGTDVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240

Query: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300
           NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDE+YLDYVAGSNGKLYAKWGSPT
Sbjct: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDETYLDYVAGSNGKLYAKWGSPT 300

Query: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360
           WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL
Sbjct: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360

Query: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420
           IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGD+YTPADLRKFAKSQADYVLGN
Sbjct: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDSYTPADLRKFAKSQADYVLGN 420

Query: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480
           NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF
Sbjct: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480

Query: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523
           LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT
Sbjct: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523

BLAST of Cucsat.G12896 vs. ExPASy TrEMBL
Match: A0A6J1CHM1 (Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111011575 PE=3 SV=1)

HSP 1 Score: 980 bits (2533), Expect = 0.0
Identity = 475/522 (91.00%), Postives = 504/522 (96.55%), Query Frame = 0

Query: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60
           MGEKSRSRGCCGWFLVLV+L L+AGAIVFAVKKK SHSDD+ APVPGPPGAIEKKYSDAL
Sbjct: 1   MGEKSRSRGCCGWFLVLVILGLVAGAIVFAVKKKMSHSDDEAAPVPGPPGAIEKKYSDAL 60

Query: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120
           KMAMKFFDVQKSG+LVDN ISWRGDSGLKDGS+A LDLSKGMYDAGDHMKFGFPMAFTAT
Sbjct: 61  KMAMKFFDVQKSGELVDNGISWRGDSGLKDGSEAKLDLSKGMYDAGDHMKFGFPMAFTAT 120

Query: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180
           VLSWSILEYGDQMDAVGQLD AKDSLKWITDYLINAHPS+NVLYIQVGDP+VDH CWDRP
Sbjct: 121 VLSWSILEYGDQMDAVGQLDPAKDSLKWITDYLINAHPSKNVLYIQVGDPKVDHGCWDRP 180

Query: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240
           ETM EKRPL QINASVPGTEVAAETAAAMA+ASLVYK+++PSYSS LLKHA+ELFSFADE
Sbjct: 181 ETMTEKRPLRQINASVPGTEVAAETAAAMAAASLVYKETNPSYSSTLLKHAQELFSFADE 240

Query: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300
           NRG+YSI+IPEVQTYYNSSGYGDELLWAASWLYHAT+DES+LDYV G NGKLYAKWGSPT
Sbjct: 241 NRGLYSISIPEVQTYYNSSGYGDELLWAASWLYHATEDESFLDYVTGDNGKLYAKWGSPT 300

Query: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360
           WFSWDDKHAATQVLLSRL+FFGS+  SKSVQVYKKTAEAVMCGLIPESPTAT+SRTDNGL
Sbjct: 301 WFSWDDKHAATQVLLSRLSFFGSKDVSKSVQVYKKTAEAVMCGLIPESPTATTSRTDNGL 360

Query: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420
           IWVSEWNSLQHPVSSAFLA+LYSDYMLTSRT K SC+GD++TPADLRKFAKSQADYVLG 
Sbjct: 361 IWVSEWNSLQHPVSSAFLAALYSDYMLTSRTPKLSCSGDSFTPADLRKFAKSQADYVLGE 420

Query: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480
           NPLKMSYLVGYGDKFP+YVHHRGASIPADA+TKCKDGFKWLDST+PNPN+A GALVGGPF
Sbjct: 421 NPLKMSYLVGYGDKFPKYVHHRGASIPADAETKCKDGFKWLDSTKPNPNVASGALVGGPF 480

Query: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSF 522
           LN+TYIDSRNNSMQGEPSTYNSAL+VGLLSSLVTTSSAVQSF
Sbjct: 481 LNETYIDSRNNSMQGEPSTYNSALIVGLLSSLVTTSSAVQSF 522

BLAST of Cucsat.G12896 vs. ExPASy TrEMBL
Match: A0A6J1FIF5 (Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111446038 PE=3 SV=1)

HSP 1 Score: 970 bits (2507), Expect = 0.0
Identity = 470/523 (89.87%), Postives = 501/523 (95.79%), Query Frame = 0

Query: 1   MGEKSRSRGCCGWFLVLVVLALIAGAIVFAVKKKSSHSDDDPAPVPGPPGAIEKKYSDAL 60
           MGEKSRSRG CGWFLVLV+L LIAGAIVFAVKKKSSHSDD+PAPVPGPPGAI KKYSDAL
Sbjct: 1   MGEKSRSRGFCGWFLVLVILGLIAGAIVFAVKKKSSHSDDEPAPVPGPPGAIVKKYSDAL 60

Query: 61  KMAMKFFDVQKSGKLVDNQISWRGDSGLKDGSDANLDLSKGMYDAGDHMKFGFPMAFTAT 120
           K+AMKFFDVQKSGKLVDN ISWRGDSGLKDG++A LDLSKGMYDAGDH+KFGFPMAFTAT
Sbjct: 61  KIAMKFFDVQKSGKLVDNTISWRGDSGLKDGTEAKLDLSKGMYDAGDHIKFGFPMAFTAT 120

Query: 121 VLSWSILEYGDQMDAVGQLDSAKDSLKWITDYLINAHPSENVLYIQVGDPEVDHACWDRP 180
           VLSWSILEYGDQMD VGQLD AKDSLKWITDYLINAHPSENVLYIQVGDP++DHACWDRP
Sbjct: 121 VLSWSILEYGDQMDVVGQLDPAKDSLKWITDYLINAHPSENVLYIQVGDPDIDHACWDRP 180

Query: 181 ETMVEKRPLIQINASVPGTEVAAETAAAMASASLVYKKSDPSYSSKLLKHAEELFSFADE 240
           ETM  KRPL  INAS PGT+VAAETAAAMASASLVYKK+DP+YS  LLKHAEELFSFADE
Sbjct: 181 ETMTGKRPLTMINASFPGTDVAAETAAAMASASLVYKKTDPTYSDLLLKHAEELFSFADE 240

Query: 241 NRGIYSINIPEVQTYYNSSGYGDELLWAASWLYHATQDESYLDYVAGSNGKLYAKWGSPT 300
           +RG+YS +IP+VQTYYNS+GYGDELLWAASWLYHAT+DE+YL+YV+G NG+LYAKWGSPT
Sbjct: 241 HRGLYSTSIPKVQTYYNSTGYGDELLWAASWLYHATEDETYLNYVSGDNGRLYAKWGSPT 300

Query: 301 WFSWDDKHAATQVLLSRLNFFGSESASKSVQVYKKTAEAVMCGLIPESPTATSSRTDNGL 360
           WFSWDDKHAATQVLLSRLNFFGS+  SKSVQVYKKTAEAVMCGLIPESPTATSSRTD+GL
Sbjct: 301 WFSWDDKHAATQVLLSRLNFFGSQGVSKSVQVYKKTAEAVMCGLIPESPTATSSRTDDGL 360

Query: 361 IWVSEWNSLQHPVSSAFLASLYSDYMLTSRTAKFSCNGDTYTPADLRKFAKSQADYVLGN 420
           IWVSEWN+LQHPVSSAFLASLYSDYMLTSRTAKFSC+G+TYTPADLR FAKSQADYVLGN
Sbjct: 361 IWVSEWNALQHPVSSAFLASLYSDYMLTSRTAKFSCSGETYTPADLRNFAKSQADYVLGN 420

Query: 421 NPLKMSYLVGYGDKFPQYVHHRGASIPADAKTKCKDGFKWLDSTEPNPNIAYGALVGGPF 480
           NP+KMSYLVGYGDKFPQYVHHRGASIP DA TKCKDGFKWL ST+PNPNIAYGALVGGPF
Sbjct: 421 NPMKMSYLVGYGDKFPQYVHHRGASIPVDATTKCKDGFKWLLSTKPNPNIAYGALVGGPF 480

Query: 481 LNDTYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSAVQSFT 523
           LN++YIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSA+QSFT
Sbjct: 481 LNESYIDSRNNSMQGEPSTYNSALVVGLLSSLVTTSSALQSFT 523

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LCP61.4e-22672.33Endoglucanase 10 OS=Arabidopsis thaliana OX=3702 GN=At1g75680 PE=2 SV=2[more]
Q9FXI95.3e-22371.76Endoglucanase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g19940 PE=2 SV=1[more]
Q69SG51.7e-20866.03Endoglucanase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0533900 PE=2 S... [more]
Q5Z9P84.7e-18761.97Endoglucanase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0715300 PE=2 S... [more]
Q8L7I04.7e-11846.81Endoglucanase 19 OS=Arabidopsis thaliana OX=3702 GN=At4g11050 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004136389.10.0100.00endoglucanase 10 [Cucumis sativus] >KGN60025.1 hypothetical protein Csa_001861 [... [more]
XP_008466493.10.097.71PREDICTED: endoglucanase 10 [Cucumis melo] >KAA0063276.1 endoglucanase 10 [Cucum... [more]
XP_038898468.10.095.41endoglucanase 10-like [Benincasa hispida][more]
XP_022141104.10.091.00endoglucanase 10-like [Momordica charantia][more]
XP_023525365.10.090.25endoglucanase 10-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0LDK20.0100.00Endoglucanase OS=Cucumis sativus OX=3659 GN=Csa_3G872070 PE=3 SV=1[more]
A0A5D3E6M40.097.71Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G008150... [more]
A0A1S3CRJ70.097.71Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103503885 PE=3 SV=1[more]
A0A6J1CHM10.091.00Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111011575 PE=3 SV=1[more]
A0A6J1FIF50.089.87Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111446038 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001701Glycoside hydrolase family 9PFAMPF00759Glyco_hydro_9coord: 4..327
e-value: 8.1E-85
score: 285.9
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 1..340
e-value: 4.2E-104
score: 351.2
NoneNo IPR availablePANTHERPTHR22298ENDO-1,4-BETA-GLUCANASEcoord: 1..341
NoneNo IPR availablePANTHERPTHR22298:SF126ENDOGLUCANASE 2coord: 1..341
IPR018221Glycoside hydrolase family 9, His active sitePROSITEPS00592GH9_2coord: 234..260
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 2..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G12896.T1Cucsat.G12896.T1mRNA
Cucsat.G12896.T2Cucsat.G12896.T2mRNA
Cucsat.G12896.T3Cucsat.G12896.T3mRNA
Cucsat.G12896.T4Cucsat.G12896.T4mRNA
Cucsat.G12896.T5Cucsat.G12896.T5mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030245 cellulose catabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008810 cellulase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds