Cucsat.G12875 (gene) Cucumber (B10) v3

Overview
NameCucsat.G12875
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Locationctg1838: 6185125 .. 6190541 (-)
RNA-Seq ExpressionCucsat.G12875
SyntenyCucsat.G12875
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAATTAGGAACCATTTTTTATTTCTCCTAAGAAAAAAAAAAAGAAAAAGAAAGAAAAGACTTGAGTAGAAAATTGAAAAACATCAACTCACCACACTGCCGGCGTATTTCGTCGATTCCCGGACGTTGCGCTCTCTTGCCTGAGGCAACTTGTTCCAAAGTTGTGAAGCTAGTAGCCTTTCCGTTCCGGCTAAACCATTTGACTCAATATTTTGAATTTTATTATTCTATTCCATCAGGAGACCGATGGTTTGAACAATTCATTGTACTCAACAACAGCTTTTTTGTTATTCAAAATCCAGGATCTGATATTCCTGCTACTGCAACTACACCATCTGAATCTCATTATTTGGTGGACTTTTTCCCTCTCTAAATCTAGATATTGATTCTGCTTCGTATTTAAGAGGTAGACGAGTATCCATCCACTTTGATTCGGACGCCAGCATCGGATTTCTGAGCTGAGTATTCGTAGTTTTTTTTTTTTTTTTTCATTTTTAAACTTCAGTTGTAAGATTATCAGATCTTTGTATATTTCTTCGATTTTGTATGTTATGCGTTCCAATTTAGTTCTGTTTTCGCAATCTTGTTCTATTAGTTCGTATGCTTTATTTTCCCTGCGTTCTGTCTGGGAATCGGATTTCAATCCGCGTAGTATATTTGTAACTTCAAGATTTTCCGAGAACTATCGGGTGCCGCATGGCTATAGCTCGGCTAGCTCGCCAAGCTAAGCGCTCGTATGGGTTTTGCGCGAAGTTGACGGCAGTGGTTATTTTAGGCCTTAGCTTTATAGTCGTTTGGTCTGTTTTTGCTTCTCCTTCCACGTCCGTGACTATTCAGAGGGAAAGTTTCGACAATATTGGCGAACCCGTAACAGGAAACACGAAAGTAAGCCATCCTAGAACTCAAAATGATAATAGGAAGAAGATAGATGAGGGCAAATTGAGCAGGGATACGAAAGATAAAGTGAAATCTGATCTAGATGGAAGGGATACGAAAAAGGTTAACGGTTCAGATTCCAAGTCTCCCAGTAACCATAACCATGCATCTGAAAAAAAGCATGGAGCTGCGAAAGAGAAAAACGAGAAACATAAAGAAAACAAACCAGAGGTTACAAGAAAAGAGAGTCAAGGATCAGAGGAATCTGAGGATGAAGATGCAGAAAAGGGAAATGAGGAGGAAGAACAGGAAGTGGTTGATGGTCAAGAAGGAGAGCTAAAAGATGATGAAGCTGAAACAGAAGGTGATCTGGGTGAGTCAGATCAGGAACCCGAGGATAGGATTGAGCCAAAAAATACAGGGAAAAAATTCAAGAGAAAAGGTCCATTGTTTGATCCAAATGCTCATTATAGTTGGAAACTATGCCGGGCAAGAAGTAAATACAATTACATCCCTTGTATTGACATTGAATCTGGAGTGGCAAGACAACAGGGCTATCGGCATAGGGAAAGGAGTTGCCCTAGAGCACCTCCACTGTGCCTTGTGCCGCTTCCTCCCAGTGGATACAAGCCCCCGGTGCACTGGCCGGAGAGCAATTCAAAGGTATCAAAGAATACATTTGTTAGGTCTTAAATTATCATTTTTTGAGGATATGTGAATGATATATATTTGTTAGTTTATAGATTATCACTTTTGAGGATACGTGAATTATTATCACGTCCTTTCTAAAAGCAATAAATTTTGCAGATACTTTACAAGAATGTGGCACACCCAAAACTTGATGCTTTCATTAAGAAACATGATTGGTTGGTGGAAGTTGGAGAGTTCCTTACATTTCCTCAGAATCATTCTGAACTCAACGGTGGAGTTATCCACTATCTCGAGTCCATTGAAGAGGTTAGCTTTTGACATCGTGCTTAAAAGCTGGTCCATTATTTTAATTTTCATGATCTATCGGTTTGGAAAAGGCAATATAACTCCTTCCCAATTAAGTCCTGCTTTTTCATGTCTAATACTGTTACTGATTTGTGGGTGGATATCAAGTGAAAGGCATGCAATCTCTCCATAGCTTAACTGTCTTGTATGATAATGTGATGGAATGTACTTTTGTTCTCCTTTGCTTAGTCAGATTGCAACAAAATCAGAGAAATAAGAAACAAAATCATGAAGAAACAAAATCATGTTGGTATTTTGGAAGCATAAAGCAAAACTTTCCGTTTATTCATTTTCTTTTTCAGACAATATTGAAAACCTCACGTTGCATCATTATATTGAAGACCTCACATTTATAACTCTGGAATGAGTGTATAATTTCTTCGTTGATTTCTTTCACTTTAAAAGTAAAGGTGTGCATTTGATTTGGTTTCTCTACTTCTCCAGGGAATTATGTTTCTATTCATGGATTGAAATTTGAATACATTTGGCAGATGGTACCCGATATTGAGTGGGGCAAGAACATTCACGTGGTTCTTGAAATTGGGTGCACATATGCAAGTTTAGGGGCTTCTCTTCTTGAAAAGAATGTTATAACATTGTCATTGGGCTTGAAGGATGACCTTGTGGACTTGGCTCAAGTTGCACTTGAGCGGGGGTTTCCAACTCTGGTTAGCCCTTTTGGGAATAGAAGACTTGCTTTTCCTAGTGGTGTTTTTGATGCCATTCATTGTGGGGGATGCAGTAGAAGTTGGCATTCCAAAAGTTTGAGCACCTTACCTTGTCTTCTTGAAATTTATTCACAAACAAGTAAAGGCCTTATCTAATAAATGGTCTTATTCTTTTTCTCTCTTTCTCTAGATGGGAAGCTTCTCCTAGAAATGAATAGAATTTTAAGGCCTGGCGGATACTTCATCTTGTCCAGTAAACATGACAGCATTGAAGAAGAAGAAGGTTTGACATGTTCTTCCAAGTTTTTACTTCATTTGCGTTAGAATTTATTCCAATTCATTTCTAATTCTCAAATTAGGATTTCGTGCACTTCTGTACTAAGATTAGAAGATTTATTGATAATTCAAATGTCAGTATTTGTGGAGAAAAGGGCATACAGTAGATGTTCAATTCTGAGATGTATATTTGTGAAATGTTGCCTTAACCGAATAACGAGTCTTCTTGCTTGCACCTCTTTTTTTGTTGAGTAGTAGATATTTAACATTCTTCTTCTTCAGCTCTCATATATTGCTTGCTTGTAAATTCTCTTTCAGCAATGAGTTCATTGACGGCTTCAATTTGTTGGAACATTCTGGCGCATAAAACTGACGAAGTCAGTGAAGTGGGTGTTAAGATATATCAGAAACCTGAATCAAACGATATATTTGAGTTGAGAAGGAAAAATCCACCTCTCTGCAAGGAAAACGAAAACCCTGATGCTACCTGGTAATTCTTTTTCAATGAACATGCCCTAGAAAGATTTGATATTCTTATTAGGTGCCATAAACTTTTTTGTCCAAAAGTTTGTATTTGATCTCAAATAATTCAAGAAAACTAGATCAGAAGAGTAATAAATCAAGAGAAGGATCAGGGCCATTGCACCGAGAAACATTTGAAGCAAAGCAACCCACATTGCAATTTCAATGTACTTCAATGATCTTGATTACTTTTCCAAAATTGCCCAACTCACATTTTGAAATTCGAATCTGCACATGATAATAATCACGAGGAAAAAAATTCTGGAGAAAATTGTAAAAACTATCCTTGAAGTATGGCGGTACATTGGTAGTTGCAATTATATAAATGTTAAATTGAGCTCTTCAAACTTACATAGCTATAGAAATTGTAACAATCGTATAAATTTGAGAGCTTAATTTTTTATCATTTGTAATTGTGGGTCCAATTTTTATTTCTATGGTAAGTTTGAGGATTCAATTTTAAAACTCATTAAATTTGAGGGCTCAATTTTTATCCTGAAGTTTAAGGGTGTTACTGCAATTACCACTATACTTGAGGGATGGTTTTTGCAATTTGCTTAAAATTCTACAACTAATTACCATGTTCAAGATGTAGTCTATACTTATACGAAGTTGATGTTATTTACTCGTACTTGAGTACCATCTTCATTTTCTGCTGTGATACGTGGACCTTTTCATTTTGGCATGATAATTTCTGTTTAACAGGTACGTGCCTATGACAACCTGCTTACACACCGTTCCAACTTCTATTGAACAGCGTGGAGCAGAATGGCCTGAAGAATGGCCGAAGAGACTTGAAACTTTCCCTGAGTGGTTGAGCAATGACAAAGAGAAGTTAATCGCAGATACCAATCTCTGGAAAGCAATTGTTGAGAAGTCATATCTCACTGGAATAGGTATTGACTGGCCAAGTGTTCGAAATGTGATGGACATGAAAGCCATTTATGGAGGGTATGATATCATCAAACTGCTTAATATCATAGACTTACCTTTCAATACTATTTTGCTTGATATAGAACTTATAAGTTAACTGGTAAATCAAAGATTTAGCAGCGTCAATTTTATGCTTTATTAAGAATTTAAGTCTTCTTCCCACTTCTTTCTTTGTACTAAAAACAGAGAGAACGAGATTAGAAAAGAATATGTTCGTTAATTCTGGCATGCATTTCCAGGTTTGCTGCTGCTGTCTCTCAGCAGAAGGTTTGGGTAATGAATGTGATCCCAGTCCATGCACCAGATACACTTCCGATAATCTTCGAACGTGGTCTGGTTGGTGTCTACCATGACTGGTGTGAGTCTTTTGGTACTTATCCACGATCATACGACCTTCTGCATGCCGATCATTTGTTCTCAAGGCTGAAGAACAGGTGAGAGAAGGTTCAACATCTTTGTTTTTGTTCCATGCTTTCTAGTAACAACTGTTGTACCTTCAAATAATATAAAACACTTGTGTTAACACCAAATTGCCACATCAACAATGTAGGTGCAAGGAGCCTGTAGCAATCGTGGTTGAGATGGATCGTATATTACGACCTGGAGGTTGGGCAATTATACGTGAAAAGGTGGTAATTATGAATCCATTGGAAGAGATACTCAAGAGTCTACAATGGAAGATTCAGATGAGTTATTCTCACGGAGATGAGGGAATCCTATGTGCGCAGAAGACCATATGGCGGCCTTAAAACAAATTTCCAGGTGAAACCTTTTTGTTCTAATAGCTGTCTCAAAAATTAAACAAGTTCCAAGCTCATTTATTACAAATTGCAGAAGGTGAAGGCAGAATATTTGCAAACAAAAGATTCTCATTCTGGTTCCTCTTCCTTTCTTCTATTTGAATTTGTATCATTTACTACTAACTGGGAAACTGATTTTTTTCCCCCTTAAATTGTTGGGATTGAGTTTAATTTGTAAAGAGGGTTGGAGAAGGGGAAATTTTTTATTTTTTATTTTTTATTTTCACCTATCATTCTCTAAAAGAACTAATGTATTGTATATCATTTCATAATTTTGTGTCGTGTTATTATAATCAGATTTATTGATAACACGTATATATAAACAAGGCTAAGAAATCTTCAATTCTCAAGATTACAATGCACTTGAAAAATGCATGTCTCTAATATTAGATTTAGT

Coding sequence (CDS)

ATGGCTATAGCTCGGCTAGCTCGCCAAGCTAAGCGCTCGTATGGGTTTTGCGCGAAGTTGACGGCAGTGGTTATTTTAGGCCTTAGCTTTATAGTCGTTTGGTCTGTTTTTGCTTCTCCTTCCACGTCCGTGACTATTCAGAGGGAAAGTTTCGACAATATTGGCGAACCCGTAACAGGAAACACGAAAGTAAGCCATCCTAGAACTCAAAATGATAATAGGAAGAAGATAGATGAGGGCAAATTGAGCAGGGATACGAAAGATAAAGTGAAATCTGATCTAGATGGAAGGGATACGAAAAAGGTTAACGGTTCAGATTCCAAGTCTCCCAGTAACCATAACCATGCATCTGAAAAAAAGCATGGAGCTGCGAAAGAGAAAAACGAGAAACATAAAGAAAACAAACCAGAGGTTACAAGAAAAGAGAGTCAAGGATCAGAGGAATCTGAGGATGAAGATGCAGAAAAGGGAAATGAGGAGGAAGAACAGGAAGTGGTTGATGGTCAAGAAGGAGAGCTAAAAGATGATGAAGCTGAAACAGAAGGTGATCTGGGTGAGTCAGATCAGGAACCCGAGGATAGGATTGAGCCAAAAAATACAGGGAAAAAATTCAAGAGAAAAGGTCCATTGTTTGATCCAAATGCTCATTATAGTTGGAAACTATGCCGGGCAAGAAGTAAATACAATTACATCCCTTGTATTGACATTGAATCTGGAGTGGCAAGACAACAGGGCTATCGGCATAGGGAAAGGAGTTGCCCTAGAGCACCTCCACTGTGCCTTGTGCCGCTTCCTCCCAGTGGATACAAGCCCCCGGTGCACTGGCCGGAGAGCAATTCAAAGATACTTTACAAGAATGTGGCACACCCAAAACTTGATGCTTTCATTAAGAAACATGATTGGTTGGTGGAAGTTGGAGAGTTCCTTACATTTCCTCAGAATCATTCTGAACTCAACGGTGGAGTTATCCACTATCTCGAGTCCATTGAAGAGATGGTACCCGATATTGAGTGGGGCAAGAACATTCACGTGGTTCTTGAAATTGGGTGCACATATGCAAGTTTAGGGGCTTCTCTTCTTGAAAAGAATGTTATAACATTGTCATTGGGCTTGAAGGATGACCTTGTGGACTTGGCTCAAGTTGCACTTGAGCGGGGGTTTCCAACTCTGGTTAGCCCTTTTGGGAATAGAAGACTTGCTTTTCCTAGTGGTGTTTTTGATGCCATTCATTGTGGGGGATGCAGTAGAAGTTGGCATTCCAAAAGTTTGAGCACCTTACCTTGTCTTCTTGAAATTTATTCACAAACAAGTAAAGGCCTTATCTAA

Protein sequence

MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTGNTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKKHGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAETEGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGVARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHDWLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLLEKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHSKSLSTLPCLLEIYSQTSKGLI
Homology
BLAST of Cucsat.G12875 vs. ExPASy Swiss-Prot
Match: Q9LN50 (Probable methyltransferase PMT28 OS=Arabidopsis thaliana OX=3702 GN=At1g19430 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 4.1e-107
Identity = 222/433 (51.27%), Postives = 289/433 (66.74%), Query Frame = 0

Query: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60
           M IA  AR+ K+  G   K+T +V+LGL F+  WS  +S +++  +QRESFD+I EPV+ 
Sbjct: 8   MGIAYFARRIKQPRGIWVKMTFIVVLGLCFVFFWSFLSSSASTFNVQRESFDDIAEPVSS 67

Query: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120
            TK +H  +++    K+ E       + KV+S    ++ KKV GS     S H H ++KK
Sbjct: 68  RTKSAHEVSES---SKLHE-------RGKVESGSKSKEGKKVGGS-----SVHKHETKKK 127

Query: 121 HGAAKEKNEKHKE-NKP---EVTRKESQGSEESEDEDAEKGNEEEEQEVV--DGQEGELK 180
              A     K K+  KP   EV  KE Q  EE+E +D+++ N+E+ +E    DG EGE  
Sbjct: 128 KEHAVSHPHKKKDVPKPVVEEVVVKEDQEHEEAESDDSDQSNKEDGEEGTESDGNEGE-- 187

Query: 181 DDEAETEGDLGESDQEPEDRIEPKN-------TGKKFKRKGPLFDPNAHYSWKLCRARSK 240
             +   +G + +S    ++ +E KN         KK KRKGP+FDP A YSW+LC  RSK
Sbjct: 188 -SDGNGDGSVDDSSASVDEEVEEKNEEVTVNEISKKRKRKGPVFDPKAEYSWRLCNTRSK 247

Query: 241 YNYIPCIDIESGVARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNV 300
           +NY+PCID +  + R Q YRHRERSCP+ P +CLVPLP  GY PPV WPES SKILYKNV
Sbjct: 248 HNYMPCIDNDGLIGRLQSYRHRERSCPKKPVMCLVPLPHDGYDPPVSWPESKSKILYKNV 307

Query: 301 AHPKLDAFIKKHDWLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLE 360
           AHPKL A+IKKH+W+ E GE+L+FPQN +  NG V+ YLE I+EMVPDIEWGKN+ +VL+
Sbjct: 308 AHPKLAAYIKKHNWVNETGEYLSFPQNQTTFNGNVLQYLEFIQEMVPDIEWGKNVRIVLD 367

Query: 361 IGCTYASLGASLLEKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFD 420
           IGC+ +S  A+LL+K+V+T+SLGLKDDLVDLAQVALERGFPT VS   +RRL FPSGVFD
Sbjct: 368 IGCSDSSFVAALLDKDVLTVSLGLKDDLVDLAQVALERGFPTFVSSLASRRLPFPSGVFD 422

BLAST of Cucsat.G12875 vs. ExPASy Swiss-Prot
Match: Q9SIZ3 (Probable methyltransferase PMT23 OS=Arabidopsis thaliana OX=3702 GN=At2g40280 PE=2 SV=2)

HSP 1 Score: 207.6 bits (527), Expect = 2.9e-52
Identity = 97/203 (47.78%), Postives = 126/203 (62.07%), Query Frame = 0

Query: 219 WKLCRARSKYNYIPCIDIESGVARQQGYR---HRERSCPRAPPLCLVPLPPSGYKPPVHW 278
           W LC+     +YIPC+D  + + + +  R   HRER CP   P CL+PL P  YKPPV W
Sbjct: 80  WDLCKGAESVDYIPCLDNYAAIKQLKSRRHMEHRERHCPEPSPKCLLPL-PDNYKPPVPW 139

Query: 279 PESNSKILYKNVAHPKLDAFIKKHDWLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPD 338
           P+S   I Y NV HPKL  + K+ +W+ + GEFL FP   ++   GV HY+E IE+ +P 
Sbjct: 140 PKSRDMIWYDNVPHPKLVEYKKEQNWVKKEGEFLVFPGGGTQFKFGVTHYVEFIEKALPS 199

Query: 339 IEWGKNIHVVLEIGCTYASLGASLLEKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFG 398
           I+WGKNI VVL++GC  AS G SLL+K+VIT+S   KD+     Q ALERG P  +S  G
Sbjct: 200 IKWGKNIRVVLDVGCGVASFGGSLLDKDVITMSFAPKDEHEAQIQFALERGIPATLSVIG 259

Query: 399 NRRLAFPSGVFDAIHCGGCSRSW 419
            ++L FPS  FD IHC  C   W
Sbjct: 260 TQQLTFPSNAFDLIHCARCRVHW 281

BLAST of Cucsat.G12875 vs. ExPASy Swiss-Prot
Match: Q8L7V3 (Probable methyltransferase PMT26 OS=Arabidopsis thaliana OX=3702 GN=At5g64030 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 3.1e-46
Identity = 132/387 (34.11%), Postives = 185/387 (47.80%), Query Frame = 0

Query: 50  SFDNIGEPVTGNTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKS 109
           S +   E   G  +    +++N     +DE K  +D  D+   D + + TK     +   
Sbjct: 124 SSETKSETEGGEDQKDDSKSENGGGGDLDEKKDLKDNSDEENPDTNEKQTKPETEDNELG 183

Query: 110 PSNHNHASEKKHGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQ 169
               N    +     K+  +  K++  +   KE++   E  +   EK N E   +V   Q
Sbjct: 184 EDGENQKQFESDNGEKKSIDDDKKSSDD--DKENKTGNEDTETKTEKENTETNVDVQVEQ 243

Query: 170 EGELKDDEAETEGDLGESDQEPE--------------DRIEPKNTGKKFKRKGPLFDPNA 229
           EG+ K+   ET GDL     + E                 E KN  +  K  G   D   
Sbjct: 244 EGQSKN---ETSGDLSPPGAQLELLNETTAQNGSFSTQATESKNEKEAQKGSGDKLD--- 303

Query: 230 HYSWKLCRARSKYNYIPCIDIESGV---ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPP 289
            Y W LC   +  +YIPC+D    +      + Y HRER CP +PP CLVPL P GYK P
Sbjct: 304 -YKWALCNTTAGPDYIPCLDNVQAIRSLPSTKHYEHRERHCPDSPPTCLVPL-PDGYKRP 363

Query: 290 VHWPESNSKILYKNVAHPKLDAFIKKHDWLVEVGEFLTFPQNHSELNGGVIHYLESIEEM 349
           + WP+S  KI Y NV H KL  +    +W+   GE+LTFP   ++   G +HY++ I+E 
Sbjct: 364 IEWPKSREKIWYTNVPHTKLAEYKGHQNWVKVTGEYLTFPGGGTQFKHGALHYIDFIQES 423

Query: 350 VPDIEWGKNIHVVLEIGCTYASLGASLLEKNVITLSLGLKDDLVDLAQVALERGFPTLVS 409
           VP I WGK   VVL++GC  AS G  L +++VIT+SL  KD+     Q ALERG P + +
Sbjct: 424 VPAIAWGKRSRVVLDVGCGVASFGGFLFDRDVITMSLAPKDEHEAQVQFALERGIPAISA 483

Query: 410 PFGNRRLAFPSGVFDAIHCGGCSRSWH 420
             G  RL FP  VFD +HC  C   WH
Sbjct: 484 VMGTTRLPFPGRVFDIVHCARCRVPWH 500

BLAST of Cucsat.G12875 vs. ExPASy Swiss-Prot
Match: Q9SD39 (Probable methyltransferase PMT27 OS=Arabidopsis thaliana OX=3702 GN=At3g51070 PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.6e-42
Identity = 129/388 (33.25%), Postives = 205/388 (52.84%), Query Frame = 0

Query: 55  GEPVTGNTKVSH-PRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNH 114
           G+    NT V+    T+ +   +  +G    +T +  K++ +G+  ++ +G++     N 
Sbjct: 203 GQEQDSNTDVTFTDATKQEQPMETGQG----ETSETSKNEENGQPEEQNSGNEETGQQNE 262

Query: 115 NH--ASEKKHGAAKEKNEKHKENKPEVTRKESQGSEE----SEDEDAEKGNE-EEEQEVV 174
               ASE+     K   +++ + +   T +E  G++E    S+DE+ E+  E ++E++  
Sbjct: 263 EKTTASEENGKGEKSMKDENGQQEEHTTAEEESGNKEEESTSKDENMEQQEERKDEKKHE 322

Query: 175 DGQEG---------ELKDDEAETEGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHY 234
            G E          E  + +   +    ES  E + +    NT ++      + D NA  
Sbjct: 323 QGSEASGFGSGIPKESAESQKSWKSQATESKDEKQRQTSESNTVER------IMDGNA-- 382

Query: 235 SWKLCRARSKYNYIPCIDIESGVAR---QQGYRHRERSCPRAPPLCLVPLPPSGYKPPVH 294
            W LC A +  +YIPC+D E  + +   ++ + HRER CP  PP CLVPL P GYK  + 
Sbjct: 383 -WVLCNATAGTDYIPCLDNEEAIMKLRSRRHFEHRERHCPEDPPTCLVPL-PEGYKEAIK 442

Query: 295 WPESNSKILYKNVAHPKLDAFIKKH-DWLVEVGEFLTFPQNHSELNGGVIHYLESIEEMV 354
           WPES  KI Y NV H KL A +K H +W+   GEFLTFP   ++   G +HY++ +++ +
Sbjct: 443 WPESRDKIWYHNVPHTKL-AEVKGHQNWVKVTGEFLTFPGGGTQFIHGALHYIDFLQQSL 502

Query: 355 PDIEWGKNIHVVLEIGCTYASLGASLLEKNVITLSLGLKDDLVDLAQVALERGFPTLVSP 414
            +I WGK   V+L++GC  AS G  L E++VI +SL  KD+     Q ALER  P + + 
Sbjct: 503 KNIAWGKRTRVILDVGCGVASFGGFLFERDVIAMSLAPKDEHEAQVQFALERKIPAISAV 562

Query: 415 FGNRRLAFPSGVFDAIHCGGCSRSWHSK 422
            G++RL FPS VFD IHC  C   WH++
Sbjct: 563 MGSKRLPFPSRVFDLIHCARCRVPWHNE 575

BLAST of Cucsat.G12875 vs. ExPASy Swiss-Prot
Match: Q6NPR7 (Probable methyltransferase PMT24 OS=Arabidopsis thaliana OX=3702 GN=At1g29470 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.3e-41
Identity = 136/446 (30.49%), Postives = 214/446 (47.98%), Query Frame = 0

Query: 1   MAIARLAR-QAKRSYGFCAKLTAVVILGLSFIVVW---SVFASPSTSV---------TIQ 60
           MA+ + +R   K+S G+   +T V+I+ L  +  W   S +++P+ S+          + 
Sbjct: 1   MAMGKYSRVDGKKSSGYGLTITIVLIVSLCLVGAWMFMSSWSAPTESIDFSANERTKDVD 60

Query: 61  RESFDNIGEPVTGNTKVSHPRTQND--------NRKKIDEGKLSRDTK-DKVKSDLDGRD 120
               D   E V   +K S P  +N+        N +K D  K   +   +K +S  + ++
Sbjct: 61  TTKSDFKSEEVDRGSK-SFPDEKNEETEVVTETNEEKTDPEKSGEENSGEKTESAEERKE 120

Query: 121 TKKVNGSDSKSPSNHNHASEKKHGAAKEKNEKHKENKPEVTRKE-SQGSEESEDEDAEKG 180
               NG   +   +    +E +    K+K +   E   E  + E S G+EE+  E  E  
Sbjct: 121 FDDKNGDGDRKNGDGEKDTESESDETKQKEKTQLEESSEENKSEDSNGTEENAGESEENT 180

Query: 181 NEEEEQEVVDGQEGELKDDEAETEGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHY 240
            ++ E+   + +E   K  +    GD  E  +E        +T +  + +       +  
Sbjct: 181 EKKSEENAGETEESTEKSKDVFPAGDQAEITKESSTGSGAWST-QLVESQNEKKAQVSSI 240

Query: 241 SWKLCRARSKYNYIPCIDIESGVAR---QQGYRHRERSCPRAPPLCLVPLPPSGYKPPVH 300
            WK+C   +  +YIPC+D    + +    + Y HRER CP   P CLV L P GYK  + 
Sbjct: 241 KWKVCNVTAGPDYIPCLDNWQAIRKLHSTKHYEHRERHCPEESPRCLVSL-PEGYKRSIK 300

Query: 301 WPESNSKILYKNVAHPKLDAFIKKH-DWLVEVGEFLTFPQNHSELNGGVIHYLESIEEMV 360
           WP+S  KI Y N+ H KL A +K H +W+   GE+LTFP   ++   G +HY++ ++E  
Sbjct: 301 WPKSREKIWYTNIPHTKL-AEVKGHQNWVKMSGEYLTFPGGGTQFKNGALHYIDFLQESY 360

Query: 361 PDIEWGKNIHVVLEIGCTYASLGASLLEKNVITLSLGLKDDLVDLAQVALERGFPTLVSP 420
           PDI WG    V+L++GC  AS G  L +++V+ LS   KD+     Q ALERG P + + 
Sbjct: 361 PDIAWGNRTRVILDVGCGVASFGGYLFDRDVLALSFAPKDEHEAQVQFALERGIPAMSNV 420

BLAST of Cucsat.G12875 vs. NCBI nr
Match: XP_031738482.1 (probable methyltransferase PMT28 [Cucumis sativus] >KGN59955.1 hypothetical protein Csa_002535 [Cucumis sativus])

HSP 1 Score: 825 bits (2131), Expect = 3.86e-296
Identity = 421/422 (99.76%), Postives = 422/422 (100.00%), Query Frame = 0

Query: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60
           MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG
Sbjct: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60

Query: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120
           NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK
Sbjct: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120

Query: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180
           HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET
Sbjct: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180

Query: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240
           EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV
Sbjct: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240

Query: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300
           ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD
Sbjct: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300

Query: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360
           WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL
Sbjct: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360

Query: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420
           EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS
Sbjct: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420

Query: 421 KS 422
           K+
Sbjct: 421 KN 422

BLAST of Cucsat.G12875 vs. NCBI nr
Match: XP_008466569.1 (PREDICTED: probable methyltransferase PMT28 isoform X2 [Cucumis melo])

HSP 1 Score: 791 bits (2042), Expect = 9.84e-285
Identity = 403/421 (95.72%), Postives = 411/421 (97.62%), Query Frame = 0

Query: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60
           MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG
Sbjct: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60

Query: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120
           NTKVSHP+TQNDNRKKIDEG LSR+TKDKVKSDL G DTKKVNGSDSKSPSNHNHASEKK
Sbjct: 61  NTKVSHPKTQNDNRKKIDEGNLSRNTKDKVKSDLGGSDTKKVNGSDSKSPSNHNHASEKK 120

Query: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180
           HGAAKEKNEKHKENKPEVTRK +QGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET
Sbjct: 121 HGAAKEKNEKHKENKPEVTRKGNQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180

Query: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240
           EGDLGESDQEPEDRIEPK+TGKKFKRKGPLFDPNA YSWKLCRARSKYNYIPCIDIESGV
Sbjct: 181 EGDLGESDQEPEDRIEPKDTGKKFKRKGPLFDPNARYSWKLCRARSKYNYIPCIDIESGV 240

Query: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300
            RQQGYRHRERSCP+APPLC+VPLPPSGY+PPVHWPESNSKILYKNVAHPKL AFIKKHD
Sbjct: 241 GRQQGYRHRERSCPKAPPLCVVPLPPSGYRPPVHWPESNSKILYKNVAHPKLAAFIKKHD 300

Query: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360
           WLV  GEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL
Sbjct: 301 WLVAAGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360

Query: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420
           EKNVITLSLGLKDDLVDLAQVALERGFPT+VSPFGNRRL FPSGVFDAIHCGGCSRSWHS
Sbjct: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTVVSPFGNRRLPFPSGVFDAIHCGGCSRSWHS 420

BLAST of Cucsat.G12875 vs. NCBI nr
Match: XP_008466568.1 (PREDICTED: probable methyltransferase PMT28 isoform X1 [Cucumis melo] >TYK19635.1 putative methyltransferase PMT28 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 791 bits (2042), Expect = 1.35e-282
Identity = 403/421 (95.72%), Postives = 411/421 (97.62%), Query Frame = 0

Query: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60
           MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG
Sbjct: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60

Query: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120
           NTKVSHP+TQNDNRKKIDEG LSR+TKDKVKSDL G DTKKVNGSDSKSPSNHNHASEKK
Sbjct: 61  NTKVSHPKTQNDNRKKIDEGNLSRNTKDKVKSDLGGSDTKKVNGSDSKSPSNHNHASEKK 120

Query: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180
           HGAAKEKNEKHKENKPEVTRK +QGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET
Sbjct: 121 HGAAKEKNEKHKENKPEVTRKGNQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180

Query: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240
           EGDLGESDQEPEDRIEPK+TGKKFKRKGPLFDPNA YSWKLCRARSKYNYIPCIDIESGV
Sbjct: 181 EGDLGESDQEPEDRIEPKDTGKKFKRKGPLFDPNARYSWKLCRARSKYNYIPCIDIESGV 240

Query: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300
            RQQGYRHRERSCP+APPLC+VPLPPSGY+PPVHWPESNSKILYKNVAHPKL AFIKKHD
Sbjct: 241 GRQQGYRHRERSCPKAPPLCVVPLPPSGYRPPVHWPESNSKILYKNVAHPKLAAFIKKHD 300

Query: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360
           WLV  GEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL
Sbjct: 301 WLVAAGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360

Query: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420
           EKNVITLSLGLKDDLVDLAQVALERGFPT+VSPFGNRRL FPSGVFDAIHCGGCSRSWHS
Sbjct: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTVVSPFGNRRLPFPSGVFDAIHCGGCSRSWHS 420

BLAST of Cucsat.G12875 vs. NCBI nr
Match: KAA0041622.1 (putative methyltransferase PMT28 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 791 bits (2042), Expect = 1.67e-282
Identity = 403/421 (95.72%), Postives = 411/421 (97.62%), Query Frame = 0

Query: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60
           MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG
Sbjct: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60

Query: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120
           NTKVSHP+TQNDNRKKIDEG LSR+TKDKVKSDL G DTKKVNGSDSKSPSNHNHASEKK
Sbjct: 61  NTKVSHPKTQNDNRKKIDEGNLSRNTKDKVKSDLGGSDTKKVNGSDSKSPSNHNHASEKK 120

Query: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180
           HGAAKEKNEKHKENKPEVTRK +QGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET
Sbjct: 121 HGAAKEKNEKHKENKPEVTRKGNQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180

Query: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240
           EGDLGESDQEPEDRIEPK+TGKKFKRKGPLFDPNA YSWKLCRARSKYNYIPCIDIESGV
Sbjct: 181 EGDLGESDQEPEDRIEPKDTGKKFKRKGPLFDPNARYSWKLCRARSKYNYIPCIDIESGV 240

Query: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300
            RQQGYRHRERSCP+APPLC+VPLPPSGY+PPVHWPESNSKILYKNVAHPKL AFIKKHD
Sbjct: 241 GRQQGYRHRERSCPKAPPLCVVPLPPSGYRPPVHWPESNSKILYKNVAHPKLAAFIKKHD 300

Query: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360
           WLV  GEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL
Sbjct: 301 WLVAAGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360

Query: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420
           EKNVITLSLGLKDDLVDLAQVALERGFPT+VSPFGNRRL FPSGVFDAIHCGGCSRSWHS
Sbjct: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTVVSPFGNRRLPFPSGVFDAIHCGGCSRSWHS 420

BLAST of Cucsat.G12875 vs. NCBI nr
Match: XP_038904648.1 (probable methyltransferase PMT28 isoform X2 [Benincasa hispida])

HSP 1 Score: 772 bits (1994), Expect = 1.98e-277
Identity = 391/421 (92.87%), Postives = 406/421 (96.44%), Query Frame = 0

Query: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60
           MAIARLARQAKRSYGFCAKLTAVVILGL FIVVWSVFASPSTSVTI+RESFDNIGEPVTG
Sbjct: 1   MAIARLARQAKRSYGFCAKLTAVVILGLCFIVVWSVFASPSTSVTIRRESFDNIGEPVTG 60

Query: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120
           NTK SHP  QNDNRKKIDEGKLSRDTKDKVKSD DGRDTKKVNGSDSKSPSNHNHAS+KK
Sbjct: 61  NTKASHPGAQNDNRKKIDEGKLSRDTKDKVKSDPDGRDTKKVNGSDSKSPSNHNHASDKK 120

Query: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180
           HGAAKEKNEKHKENKP VTRKE+QGSEES+DEDAEKGNEEEEQEV+DGQE ELKDDEAET
Sbjct: 121 HGAAKEKNEKHKENKPVVTRKENQGSEESDDEDAEKGNEEEEQEVIDGQEAELKDDEAET 180

Query: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240
           EGDLGESDQEPE+RIEPK+ GKK KRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIE+GV
Sbjct: 181 EGDLGESDQEPEERIEPKDKGKKVKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIEAGV 240

Query: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300
            RQQGYRHRERSCPRAPP+CLVPLPPSGY+PPVHWPESNSKILYKNVAHPKL AFIKKHD
Sbjct: 241 VRQQGYRHRERSCPRAPPMCLVPLPPSGYRPPVHWPESNSKILYKNVAHPKLAAFIKKHD 300

Query: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360
           WLVE GEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHV+LEIGCTYASLGA LL
Sbjct: 301 WLVEAGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVILEIGCTYASLGAFLL 360

Query: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420
           EK+VITLSLGLK+DLVDLAQVALERGFPT+VSPFG+RRL FPSGVFDAIHCGGCSRSWHS
Sbjct: 361 EKDVITLSLGLKNDLVDLAQVALERGFPTVVSPFGSRRLPFPSGVFDAIHCGGCSRSWHS 420

BLAST of Cucsat.G12875 vs. ExPASy TrEMBL
Match: A0A0A0LD92 (Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G855980 PE=3 SV=1)

HSP 1 Score: 825 bits (2131), Expect = 1.87e-296
Identity = 421/422 (99.76%), Postives = 422/422 (100.00%), Query Frame = 0

Query: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60
           MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG
Sbjct: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60

Query: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120
           NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK
Sbjct: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120

Query: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180
           HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET
Sbjct: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180

Query: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240
           EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV
Sbjct: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240

Query: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300
           ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD
Sbjct: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300

Query: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360
           WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL
Sbjct: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360

Query: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420
           EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS
Sbjct: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420

Query: 421 KS 422
           K+
Sbjct: 421 KN 422

BLAST of Cucsat.G12875 vs. ExPASy TrEMBL
Match: A0A1S3CRL8 (Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103503950 PE=3 SV=1)

HSP 1 Score: 791 bits (2042), Expect = 4.77e-285
Identity = 403/421 (95.72%), Postives = 411/421 (97.62%), Query Frame = 0

Query: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60
           MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG
Sbjct: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60

Query: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120
           NTKVSHP+TQNDNRKKIDEG LSR+TKDKVKSDL G DTKKVNGSDSKSPSNHNHASEKK
Sbjct: 61  NTKVSHPKTQNDNRKKIDEGNLSRNTKDKVKSDLGGSDTKKVNGSDSKSPSNHNHASEKK 120

Query: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180
           HGAAKEKNEKHKENKPEVTRK +QGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET
Sbjct: 121 HGAAKEKNEKHKENKPEVTRKGNQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180

Query: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240
           EGDLGESDQEPEDRIEPK+TGKKFKRKGPLFDPNA YSWKLCRARSKYNYIPCIDIESGV
Sbjct: 181 EGDLGESDQEPEDRIEPKDTGKKFKRKGPLFDPNARYSWKLCRARSKYNYIPCIDIESGV 240

Query: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300
            RQQGYRHRERSCP+APPLC+VPLPPSGY+PPVHWPESNSKILYKNVAHPKL AFIKKHD
Sbjct: 241 GRQQGYRHRERSCPKAPPLCVVPLPPSGYRPPVHWPESNSKILYKNVAHPKLAAFIKKHD 300

Query: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360
           WLV  GEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL
Sbjct: 301 WLVAAGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360

Query: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420
           EKNVITLSLGLKDDLVDLAQVALERGFPT+VSPFGNRRL FPSGVFDAIHCGGCSRSWHS
Sbjct: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTVVSPFGNRRLPFPSGVFDAIHCGGCSRSWHS 420

BLAST of Cucsat.G12875 vs. ExPASy TrEMBL
Match: A0A5D3D7V2 (Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G00220 PE=3 SV=1)

HSP 1 Score: 791 bits (2042), Expect = 6.55e-283
Identity = 403/421 (95.72%), Postives = 411/421 (97.62%), Query Frame = 0

Query: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60
           MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG
Sbjct: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60

Query: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120
           NTKVSHP+TQNDNRKKIDEG LSR+TKDKVKSDL G DTKKVNGSDSKSPSNHNHASEKK
Sbjct: 61  NTKVSHPKTQNDNRKKIDEGNLSRNTKDKVKSDLGGSDTKKVNGSDSKSPSNHNHASEKK 120

Query: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180
           HGAAKEKNEKHKENKPEVTRK +QGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET
Sbjct: 121 HGAAKEKNEKHKENKPEVTRKGNQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180

Query: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240
           EGDLGESDQEPEDRIEPK+TGKKFKRKGPLFDPNA YSWKLCRARSKYNYIPCIDIESGV
Sbjct: 181 EGDLGESDQEPEDRIEPKDTGKKFKRKGPLFDPNARYSWKLCRARSKYNYIPCIDIESGV 240

Query: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300
            RQQGYRHRERSCP+APPLC+VPLPPSGY+PPVHWPESNSKILYKNVAHPKL AFIKKHD
Sbjct: 241 GRQQGYRHRERSCPKAPPLCVVPLPPSGYRPPVHWPESNSKILYKNVAHPKLAAFIKKHD 300

Query: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360
           WLV  GEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL
Sbjct: 301 WLVAAGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360

Query: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420
           EKNVITLSLGLKDDLVDLAQVALERGFPT+VSPFGNRRL FPSGVFDAIHCGGCSRSWHS
Sbjct: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTVVSPFGNRRLPFPSGVFDAIHCGGCSRSWHS 420

BLAST of Cucsat.G12875 vs. ExPASy TrEMBL
Match: A0A1S3CRK5 (Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103503950 PE=3 SV=1)

HSP 1 Score: 791 bits (2042), Expect = 6.55e-283
Identity = 403/421 (95.72%), Postives = 411/421 (97.62%), Query Frame = 0

Query: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60
           MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG
Sbjct: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60

Query: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120
           NTKVSHP+TQNDNRKKIDEG LSR+TKDKVKSDL G DTKKVNGSDSKSPSNHNHASEKK
Sbjct: 61  NTKVSHPKTQNDNRKKIDEGNLSRNTKDKVKSDLGGSDTKKVNGSDSKSPSNHNHASEKK 120

Query: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180
           HGAAKEKNEKHKENKPEVTRK +QGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET
Sbjct: 121 HGAAKEKNEKHKENKPEVTRKGNQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180

Query: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240
           EGDLGESDQEPEDRIEPK+TGKKFKRKGPLFDPNA YSWKLCRARSKYNYIPCIDIESGV
Sbjct: 181 EGDLGESDQEPEDRIEPKDTGKKFKRKGPLFDPNARYSWKLCRARSKYNYIPCIDIESGV 240

Query: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300
            RQQGYRHRERSCP+APPLC+VPLPPSGY+PPVHWPESNSKILYKNVAHPKL AFIKKHD
Sbjct: 241 GRQQGYRHRERSCPKAPPLCVVPLPPSGYRPPVHWPESNSKILYKNVAHPKLAAFIKKHD 300

Query: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360
           WLV  GEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL
Sbjct: 301 WLVAAGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360

Query: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420
           EKNVITLSLGLKDDLVDLAQVALERGFPT+VSPFGNRRL FPSGVFDAIHCGGCSRSWHS
Sbjct: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTVVSPFGNRRLPFPSGVFDAIHCGGCSRSWHS 420

BLAST of Cucsat.G12875 vs. ExPASy TrEMBL
Match: A0A5A7TDZ2 (Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold93G00950 PE=3 SV=1)

HSP 1 Score: 791 bits (2042), Expect = 8.09e-283
Identity = 403/421 (95.72%), Postives = 411/421 (97.62%), Query Frame = 0

Query: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60
           MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG
Sbjct: 1   MAIARLARQAKRSYGFCAKLTAVVILGLSFIVVWSVFASPSTSVTIQRESFDNIGEPVTG 60

Query: 61  NTKVSHPRTQNDNRKKIDEGKLSRDTKDKVKSDLDGRDTKKVNGSDSKSPSNHNHASEKK 120
           NTKVSHP+TQNDNRKKIDEG LSR+TKDKVKSDL G DTKKVNGSDSKSPSNHNHASEKK
Sbjct: 61  NTKVSHPKTQNDNRKKIDEGNLSRNTKDKVKSDLGGSDTKKVNGSDSKSPSNHNHASEKK 120

Query: 121 HGAAKEKNEKHKENKPEVTRKESQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180
           HGAAKEKNEKHKENKPEVTRK +QGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET
Sbjct: 121 HGAAKEKNEKHKENKPEVTRKGNQGSEESEDEDAEKGNEEEEQEVVDGQEGELKDDEAET 180

Query: 181 EGDLGESDQEPEDRIEPKNTGKKFKRKGPLFDPNAHYSWKLCRARSKYNYIPCIDIESGV 240
           EGDLGESDQEPEDRIEPK+TGKKFKRKGPLFDPNA YSWKLCRARSKYNYIPCIDIESGV
Sbjct: 181 EGDLGESDQEPEDRIEPKDTGKKFKRKGPLFDPNARYSWKLCRARSKYNYIPCIDIESGV 240

Query: 241 ARQQGYRHRERSCPRAPPLCLVPLPPSGYKPPVHWPESNSKILYKNVAHPKLDAFIKKHD 300
            RQQGYRHRERSCP+APPLC+VPLPPSGY+PPVHWPESNSKILYKNVAHPKL AFIKKHD
Sbjct: 241 GRQQGYRHRERSCPKAPPLCVVPLPPSGYRPPVHWPESNSKILYKNVAHPKLAAFIKKHD 300

Query: 301 WLVEVGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360
           WLV  GEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL
Sbjct: 301 WLVAAGEFLTFPQNHSELNGGVIHYLESIEEMVPDIEWGKNIHVVLEIGCTYASLGASLL 360

Query: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTLVSPFGNRRLAFPSGVFDAIHCGGCSRSWHS 420
           EKNVITLSLGLKDDLVDLAQVALERGFPT+VSPFGNRRL FPSGVFDAIHCGGCSRSWHS
Sbjct: 361 EKNVITLSLGLKDDLVDLAQVALERGFPTVVSPFGNRRLPFPSGVFDAIHCGGCSRSWHS 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LN504.1e-10751.27Probable methyltransferase PMT28 OS=Arabidopsis thaliana OX=3702 GN=At1g19430 PE... [more]
Q9SIZ32.9e-5247.78Probable methyltransferase PMT23 OS=Arabidopsis thaliana OX=3702 GN=At2g40280 PE... [more]
Q8L7V33.1e-4634.11Probable methyltransferase PMT26 OS=Arabidopsis thaliana OX=3702 GN=At5g64030 PE... [more]
Q9SD391.6e-4233.25Probable methyltransferase PMT27 OS=Arabidopsis thaliana OX=3702 GN=At3g51070 PE... [more]
Q6NPR72.3e-4130.49Probable methyltransferase PMT24 OS=Arabidopsis thaliana OX=3702 GN=At1g29470 PE... [more]
Match NameE-valueIdentityDescription
XP_031738482.13.86e-29699.76probable methyltransferase PMT28 [Cucumis sativus] >KGN59955.1 hypothetical prot... [more]
XP_008466569.19.84e-28595.72PREDICTED: probable methyltransferase PMT28 isoform X2 [Cucumis melo][more]
XP_008466568.11.35e-28295.72PREDICTED: probable methyltransferase PMT28 isoform X1 [Cucumis melo] >TYK19635.... [more]
KAA0041622.11.67e-28295.72putative methyltransferase PMT28 isoform X1 [Cucumis melo var. makuwa][more]
XP_038904648.11.98e-27792.87probable methyltransferase PMT28 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0LD921.87e-29699.76Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G855980 PE=3 SV=1[more]
A0A1S3CRL84.77e-28595.72Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103503950 PE=3 SV=1[more]
A0A5D3D7V26.55e-28395.72Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G00... [more]
A0A1S3CRK56.55e-28395.72Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103503950 PE=3 SV=1[more]
A0A5A7TDZ28.09e-28395.72Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold93G009... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePFAMPF03141Methyltransf_29coord: 7..132
e-value: 1.4E-54
score: 185.6
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePANTHERPTHR10108SAM-DEPENDENT METHYLTRANSFERASEcoord: 7..137
NoneNo IPR availablePANTHERPTHR10108:SF1102METHYLTRANSFERASE PMT28-RELATEDcoord: 7..137
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 28..103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G12875.T2Cucsat.G12875.T2mRNA
Cucsat.G12875.T7Cucsat.G12875.T7mRNA
Cucsat.G12875.T6Cucsat.G12875.T6mRNA
Cucsat.G12875.T3Cucsat.G12875.T3mRNA
Cucsat.G12875.T1Cucsat.G12875.T1mRNA
Cucsat.G12875.T16Cucsat.G12875.T16mRNA
Cucsat.G12875.T13Cucsat.G12875.T13mRNA
Cucsat.G12875.T11Cucsat.G12875.T11mRNA
Cucsat.G12875.T4Cucsat.G12875.T4mRNA
Cucsat.G12875.T14Cucsat.G12875.T14mRNA
Cucsat.G12875.T12Cucsat.G12875.T12mRNA
Cucsat.G12875.T10Cucsat.G12875.T10mRNA
Cucsat.G12875.T18Cucsat.G12875.T18mRNA
Cucsat.G12875.T9Cucsat.G12875.T9mRNA
Cucsat.G12875.T8Cucsat.G12875.T8mRNA
Cucsat.G12875.T5Cucsat.G12875.T5mRNA
Cucsat.G12875.T15Cucsat.G12875.T15mRNA
Cucsat.G12875.T17Cucsat.G12875.T17mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008168 methyltransferase activity