Cucsat.G12630 (gene) Cucumber (B10) v3

Overview
NameCucsat.G12630
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionDelta-12 fatty acid desaturase
Locationctg1838: 1288854 .. 1292494 (-)
RNA-Seq ExpressionCucsat.G12630
SyntenyCucsat.G12630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCTCCAAATAAAGGAAAAATATTCACACAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAAATAAGGCTTTGTTGCCGGCGATCTGAAGGCGGCGGCCATTGCTCTCGAGAGAGAGAGAAAGAGAGAGATTGAAAGAGCGGAGAGTACGAGGCTCTCTCAAACTTCGGTCCTCTTCTTCTCTTTCAGGTATCCTTCTCTATCCCTTCCTATTCTGTTTCCGCTTTTCTCTTTCTTCGCCATCATGCTCGTTCTCTTCTTTTGTTCTCTCACTCAATGTGATTGACTTTATGTTGTTTTTCTGTTTTATTTTCCCATTAACGTTTGTTGTAATGTGTAGATCTATGATAAGATTTGAATTAGTGCTCATTAATGTGTTGCATGCTTTTGATTTCGTTTTAAGCAGAGATTACTTTGTCTATATTCATTAAATCATTGGATTTTAGGTTCTTTCAGAGTTTGTAAACAGTGATGTTAAGGAATGCTGAGATTTATGACTGATGAGAGTTCGTGTTTGTCTATTAGCTTGTTGGTTTCCTCTTTGATATCGCATGGATTCGATCTGTATGTCTGGGTTTGACTCTTGCTGAAATGGCTTCACTATAGCATATTTGAGTTTGTGTTGTTGAAGATTTGTTGATTTCTTGGAAAATTGGGAGTTCGTTTTGTTTTTCCTCTTTTTACAGGTTTATTGATTGGCGATATCTCGTTTTCAACTTCCGAAATGCTATTTTTCATAAGAAGAAACTGTGGATGTATTTTTCTACTCGATTAGAGATCCTTGAAACTATGCCAAAAGGAATTGTTTTTTTCACCAAATTGTTTTTTGTCGTTTGTGATATTAATGCATTTTCTTATTCTTAATAAAGTTTAAGTGTTCCTTTATTATTTTTTAATGGTGGTTGTTGGAATGGTTATTTCCCTTTTACTAAAAGCTTTTTCCATGTGATTCAAAGGTGTATTTGGGGTTTCCCGGTCTTTGTTCCAAGTCAATTAGGATGGGCGCCAATTCGGTTTTAGCTTCTGTATCATTGGTGTATATTTTGTTATAGGGAGGAAAAGAAGGAAGAAGAAAAATCTTCCGTCCTACAGTGTGCTGAGTAAACTCGAGCAGCCTTTTCTGCTGAATACTTTTTAAAATTATTTTTTAACTGTGATTTGGTGAACTTAAATTGTTTTAATAAATAATGTGGATTAAATCTTAACAGAAACGATTTAAAAAAAAAACATATTTTTAGTGAAATCTTTATTTTATTTAAAAGATCTCCATCAGTCCTGATGTTTCCTAGAAAACTTAAAAAATAATGGGTAGCTAGAAATATGGAAATAAATGTATTTTGAGCTTCTCCTTGAAACTGGAGCAGTGGTCAATTTTTTTTCGTTTGTGTAGATGTGATAGAAATAGAATATTCCGTTCCCTTCCGGCATCATCAGAGAGGTGGAATTGGTCTTTCTCAACCTCAGTATCAATTAAATTAAGTTTCGTGATAAATTTCAAATGTTTGGTAGGGTCAAATAGTTTGTAAAATCCCTAGCCGTCCATTATGATGCAAACGGAAGGATTTCACTTGCTCTTTTAAATTACTAAAAATATTTCGTCGTTGATGTTGCCTGTCTGTTTTATCATTTCTCTTTCCGCCTCAAGAGGCGGCTAAGTGTCTTGGCAAGTTAATTTTGTTTTGTACTTCCGCCCCTTGTCTTTTGGCCCTTTTAATTAAGTTTTTCCCTTTAATCTCCTGGTCGGCGTACATTGAATTATTAGGTTTGCATTTAATTTCTATGTTTATAAATCTGTTAAAGCAATTAATGTGGTACTGTTTGATCTAGATATTTTGAAGTCTAAAACGTTAAACCCTTTGTTTGATTTGTTTTTTTTTTTTTTTGTTCCAGGTCAATATCCTTGAAAAAATGGGTGCTGGAGGTCGAATGTCCTCTCCTTCATCTCTCAAGAAGACAGATTCTGACCACTTGAAGCGAGTACCGTCCACAAAACCCCCATTCACACTTGGTGAGCTCAAGAAAGCTATTCCACCTCATTGCTTTCAACGTTCAGTCCTTCGTTCTTTCTCCTATGTCGTTTATGACCTAACCTTAGCCTCTATCTTCTATTACATTGCAACCACATATTTCCAAAATCTTCCTAGCCTCTTATTTTATCCAGCCTGGGCTTTGTACTGGGCTGCCCAAGGCTGCGTCCTTACTGGCGTTTGGGTCATTGCCCACGAGTGTGGCCACCACGCCTTCAGTGATTACCAGTGGCTGGATGACACCGTTGGCCTTATCCTCCACTCGTTTCTCATGGTCCCCTACTTCTCTTGGAAATACAGCCACCGTCGACACCATTCCAACACCGGATCTCTTGAACGAGATGAAGTCTTTGTCCCTAAACAGAAATCCGACATGAAATGGTACTCCACATACATCAACAACCCTGTAGGCCGTGTCCTCACCCTCACCATCCAACTGGTCCTAGGCTGGCCTCTTTACTTGGCCTTCAACGTCTCTGGCCGTCCCTACGACCGTTTTGCCTGCCACTATGACCCATACGGTCCCATCTACACTGACCGTGAGCGGTTACAGATATTCATTTCGGACGCTGGGCTGCTAACTGTCACCTATGGGCTCTACCGCCTTGTTCTAGCCAAAGGATTGGCTTGGGTGGTTTGTGTTTACGGAGTTCCACTTCTGATCGTAAATGGATTCCTCGTCCTAATCACATACCTTCAACACACTCACACAGCACTGCCACATTACGATTCCTCCGAATGGGACTGGCTAAGAGGTGCCTTGGCAACCGTAGACAGAGACTACGGCATCTTGAACAAGGTCTTCCACAACATTACGGACACTCATGTGGCTCATCATTTGGTGTCCACAATGCCACATTACCATGCCATGGAAGCAACCAAGGCAATCAAGCCAATTTTGGGCCAATACTATCACTTTGATGGGACCCCGGTTGTGAAGGCAATGTGGAGAGAAGCAAAAGAGTGCATTTACGTGGAGCCTGATGAAGGTGAGAAGAAAGGCGTTTATTGGTATAAAAACAAGTTGTAATTTGCTTGTGGAAGAAGCAGTGTGAGAGAGAAAAGTGCCTTTGTCTACTTAAAAGTGGGGTAAACTTTTAAGCATCTTGGTTCTGCTACTACTACTACTCTAGGAGAGCTGTTTGTTTTTCTTCTTTGTTATATGAACCTTAGTTTCTGTTATGGGTAAGTTTAATTCCTACCAAAATGCCAACTCTTTTGTTAAGTCAGTCAAGTTTCTTGGTTGGTTTTACTTTGTTGGTTCGGGTCTAGATTACAAGATACTCGTAAGGTATGCAGATGCTTAATGATGATTAAATGTTAATGCATGCGAATTGACTTTTCTTCAGATTGCGTCTATGTTATGGATAGATTAGGATGTCCATGTGTAATATATTTTCATGTATGGAAAAACATTGCTAGACTTGGCCTCACCGTTCTTTATCCATTTATTGATTAGATGACATATCATTTATCATTTTAAATGTGGAATGGAAACATGAGGTTGGAAAAGGATAATAAAATAAGTGAACCATTGTTTTTCCTTTCATTGAATGATTGGTTTGGAAAGATTGGAGTGATCTAATATTAATGGACCACACAAATGCTTTGGAAAAAGACTGAATTTCTTTTCCACCAATCACTCTCTTGTCTTCTTGGCCGTTGGTTT

Coding sequence (CDS)

ATGGGTGCTGGAGGTCGAATGTCCTCTCCTTCATCTCTCAAGAAGACAGATTCTGACCACTTGAAGCGAGTACCGTCCACAAAACCCCCATTCACACTTGGTGAGCTCAAGAAAGCTATTCCACCTCATTGCTTTCAACGTTCAGTCCTTCGTTCTTTCTCCTATGTCGTTTATGACCTAACCTTAGCCTCTATCTTCTATTACATTGCAACCACATATTTCCAAAATCTTCCTAGCCTCTTATTTTATCCAGCCTGGGCTTTGTACTGGGCTGCCCAAGGCTGCGTCCTTACTGGCGTTTGGGTCATTGCCCACGAGTGTGGCCACCACGCCTTCAGTGATTACCAGTGGCTGGATGACACCGTTGGCCTTATCCTCCACTCGTTTCTCATGGTCCCCTACTTCTCTTGGAAATACAGCCACCGTCGACACCATTCCAACACCGGATCTCTTGAACGAGATGAAGTCTTTGTCCCTAAACAGAAATCCGACATGAAATGGTACTCCACATACATCAACAACCCTGTAGGCCGTGTCCTCACCCTCACCATCCAACTGGTCCTAGGCTGGCCTCTTTACTTGGCCTTCAACGTCTCTGGCCGTCCCTACGACCGTTTTGCCTGCCACTATGACCCATACGGTCCCATCTACACTGACCGTGAGCGGTTACAGATATTCATTTCGGACGCTGGGCTGCTAACTGTCACCTATGGGCTCTACCGCCTTGTTCTAGCCAAAGGATTGGCTTGGGTGGTTTGTGTTTACGGAGTTCCACTTCTGATCGTAAATGGATTCCTCGTCCTAATCACATACCTTCAACACACTCACACAGCACTGCCACATTACGATTCCTCCGAATGGGACTGGCTAAGAGGTGCCTTGGCAACCGTAGACAGAGACTACGGCATCTTGAACAAGGTCTTCCACAACATTACGGACACTCATGTGGCTCATCATTTGGTGTCCACAATGCCACATTACCATGCCATGGAAGCAACCAAGGCAATCAAGCCAATTTTGGGCCAATACTATCACTTTGATGGGACCCCGGTTGTGAAGGCAATGTGGAGAGAAGCAAAAGAGTGCATTTACGTGGAGCCTGATGAAGGTGAGAAGAAAGGCGTTTATTGGTATAAAAACAAGTTGTAA

Protein sequence

MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDLTLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDDTVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVLTLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLYRLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAKECIYVEPDEGEKKGVYWYKNKL
Homology
BLAST of Cucsat.G12630 vs. ExPASy Swiss-Prot
Match: Q8GZC3 (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 1.6e-192
Identity = 315/382 (82.46%), Postives = 340/382 (89.01%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRMS P   KK +S+ LKRVP +KPPFTLG+LKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSVPPPPKKLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           T+A IFYYIAT YF  LP  L Y AW +YWA QGCVLTGVWVIAHECGHHAFSDYQ LDD
Sbjct: 61  TVAFIFYYIATNYFHLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
            VGL+LHS L+VPYFSWK+SHRRHHSNT SLERDEVFVPK+KS ++W+S Y+NNP GR+ 
Sbjct: 121 IVGLVLHSCLLVPYFSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLF 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
           TLTI L LGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRER +I+ISDAG+L VT+GLY
Sbjct: 181 TLTITLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RL  AKGLAWV+CVYGVPLLIVN FLV+ITYLQHTH ++PHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLAAAKGLAWVICVYGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHL STMPHYHAMEATKAIKPILG+YY FDGTP  KAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAK 360

Query: 361 ECIYVEPDEG-EKKGVYWYKNK 382
           ECIYVE D+G E KGVYWY  K
Sbjct: 361 ECIYVEADDGDESKGVYWYNKK 382

BLAST of Cucsat.G12630 vs. ExPASy Swiss-Prot
Match: P48631 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX=3847 GN=FAD2-2 PE=2 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 6.9e-188
Identity = 309/383 (80.68%), Postives = 336/383 (87.73%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGR   P + +K++ D LKRVP  KP F+L ++KKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRTDVPPANRKSEVDPLKRVPFEKPQFSLSQIKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           T+A   YY+AT YF  LP  L +   A+YWA QGC+LTGVWVIAHECGHHAFSDYQ LDD
Sbjct: 61  TIAFCLYYVATHYFHLLPGPLSFRGMAIYWAVQGCILTGVWVIAHECGHHAFSDYQLLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
            VGLILHS L+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS +KWYS Y+NNP GRVL
Sbjct: 121 IVGLILHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSCIKWYSKYLNNPPGRVL 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
           TL + L LGWPLYLA NVSGRPYDRFACHYDPYGPIY+DRERLQI+ISDAG+L V YGL+
Sbjct: 181 TLAVTLTLGWPLYLALNVSGRPYDRFACHYDPYGPIYSDRERLQIYISDAGVLAVVYGLF 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RL +AKGLAWVVCVYGVPLL+VNGFLVLIT+LQHTH ALPHY SSEWDWLRGALATVDRD
Sbjct: 241 RLAMAKGLAWVVCVYGVPLLVVNGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHL STMPHYHAMEATKAIKPILG+YY FD TP VKAMWREA+
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREAR 360

Query: 361 ECIYVEPDEG-EKKGVYWYKNKL 383
           ECIYVEPD+  E KGV+WY NKL
Sbjct: 361 ECIYVEPDQSTESKGVFWYNNKL 383

BLAST of Cucsat.G12630 vs. ExPASy Swiss-Prot
Match: P46313 (Delta(12)-fatty-acid desaturase OS=Arabidopsis thaliana OX=3702 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 2.5e-185
Identity = 300/383 (78.33%), Postives = 336/383 (87.73%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRM  P+S KK+++D  KRVP  KPPF++G+LKKAIPPHCF+RS+ RSFSY++ D+
Sbjct: 1   MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
            +AS FYY+AT YF  LP  L Y AW LYWA QGCVLTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61  IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS +KWY  Y+NNP+GR++
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
            LT+Q VLGWPLYLAFNVSGRPYD FACH+ P  PIY DRERLQI++SDAG+L V +GLY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           R   A+G+A ++C+YGVPLLIVN FLVLITYLQHTH +LPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHL STMPHY+AMEATKAIKPILG YY FDGTP   AM+REAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360

Query: 361 ECIYVEPD-EGEKKGVYWYKNKL 383
           ECIYVEPD EG+KKGVYWY NKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383

BLAST of Cucsat.G12630 vs. ExPASy Swiss-Prot
Match: Q39287 (Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea OX=3707 PE=2 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 9.4e-185
Identity = 303/384 (78.91%), Postives = 334/384 (86.98%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRM    S KK+++D LKRVP   PPFT+GELKKAIPPHCF+RS+ RSFSY+++D+
Sbjct: 1   MGAGGRMQVSPSPKKSETDTLKRVPCETPPFTVGELKKAIPPHCFKRSIPRSFSYLIWDI 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
            +AS FYY+ATTYF  LP  L Y AW LYWA QG VLTGVWVIAHECGHHAFSDYQWLDD
Sbjct: 61  IVASCFYYVATTYFPLLPHPLSYVAWPLYWACQGVVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLI HSFL+VPYFSWKYSHRRHHSNTGSLERDEVFVPK+KSD+KWY  Y+NNP+GR +
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSDIKWYGKYLNNPLGRTV 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPY-DRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGL 240
            LT+Q  LGWPLY AFNVSGRPY + FACH+ P  PIY DRERLQI++SDAG+L V YGL
Sbjct: 181 MLTVQFTLGWPLYWAFNVSGRPYPEGFACHFHPNAPIYNDRERLQIYVSDAGILAVCYGL 240

Query: 241 YRLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDR 300
           YR   A+G+A +VC+YGVPLLIVN FLVLITYLQHTH +LPHYDSSEWDWLRGALATVDR
Sbjct: 241 YRYAAAQGVASMVCLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 300

Query: 301 DYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREA 360
           DYGILNKVFHNITDTHVAHHL STMPHYHAME TKAIKPILG YY FDGTP VKAMWREA
Sbjct: 301 DYGILNKVFHNITDTHVAHHLFSTMPHYHAMEVTKAIKPILGDYYQFDGTPWVKAMWREA 360

Query: 361 KECIYVEPD-EGEKKGVYWYKNKL 383
           KECIYVEPD +GEKKGV+WY NKL
Sbjct: 361 KECIYVEPDRQGEKKGVFWYNNKL 384

BLAST of Cucsat.G12630 vs. ExPASy Swiss-Prot
Match: Q84VT2 (Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2)

HSP 1 Score: 647.1 bits (1668), Expect = 1.2e-184
Identity = 299/387 (77.26%), Postives = 332/387 (85.79%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLK----KTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYV 60
           MGAGGRM+ P+  +    +     ++RVPS KPPFTL E+KKAIPPHCF+RS+L+SFSYV
Sbjct: 1   MGAGGRMTVPNKWEGEGDEKSQKPVQRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFSYV 60

Query: 61  VYDLTLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQ 120
           +YDLTL +IFYY+ATTY   LP  L Y AW +YWA QGCVLTGVWVIAHECGHHAFSDYQ
Sbjct: 61  LYDLTLVAIFYYVATTYIDALPGPLRYAAWPVYWALQGCVLTGVWVIAHECGHHAFSDYQ 120

Query: 121 WLDDTVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPV 180
           W+DD VGL+LHS L+VPYFSWKYSHRRHHSNTGSLERDEVFVPK KS M W+S Y+NNP 
Sbjct: 121 WVDDCVGLVLHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSKMPWFSKYLNNPP 180

Query: 181 GRVLTLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVT 240
           GRV+TL + L LGWPLYLA NVSG PYDRFACH+DPYGPIYTDRERLQI+ISD G++  T
Sbjct: 181 GRVMTLIVTLTLGWPLYLALNVSGWPYDRFACHFDPYGPIYTDRERLQIYISDVGIMAAT 240

Query: 241 YGLYRLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALAT 300
           Y LY++  A+GLAW+VCVYGVPLLIVN FLV ITYLQHTH ALPHYDSSEWDWLRGALAT
Sbjct: 241 YTLYKIAAARGLAWLVCVYGVPLLIVNAFLVTITYLQHTHPALPHYDSSEWDWLRGALAT 300

Query: 301 VDRDYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMW 360
            DRDYGILNKVFHNITDTHVAHHL STMPHYHAMEATKAIKPILG YY FDGTPV KAMW
Sbjct: 301 ADRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYQFDGTPVYKAMW 360

Query: 361 REAKECIYVEPDEG-EKKGVYWYKNKL 383
           REA+EC+YVEPD+G   KGV+WYK  L
Sbjct: 361 REARECLYVEPDDGANSKGVFWYKKNL 387

BLAST of Cucsat.G12630 vs. NCBI nr
Match: XP_004136572.1 (delta(12)-fatty-acid desaturase FAD2 [Cucumis sativus] >KGN59307.1 hypothetical protein Csa_001023 [Cucumis sativus])

HSP 1 Score: 802 bits (2071), Expect = 1.71e-293
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
           TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVEPDEGEKKGVYWYKNKL 382
           ECIYVEPDEGEKKGVYWYKNKL
Sbjct: 361 ECIYVEPDEGEKKGVYWYKNKL 382

BLAST of Cucsat.G12630 vs. NCBI nr
Match: XP_008443062.2 (PREDICTED: delta(12)-fatty-acid desaturase FAD2 [Cucumis melo] >KAA0043728.1 delta(12)-fatty-acid desaturase FAD2 [Cucumis melo var. makuwa] >TYK25404.1 delta(12)-fatty-acid desaturase FAD2 [Cucumis melo var. makuwa])

HSP 1 Score: 787 bits (2032), Expect = 1.51e-287
Identity = 372/382 (97.38%), Postives = 378/382 (98.95%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRMSSPSSLKK+DSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           TLASIFYY+ATTYFQ+LPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS MKWYSTY+NNPVGRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
           TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIY+DRERLQIFISDAGLL V YGLY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RLVLAKG AWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVEPDEGEKKGVYWYKNKL 382
           ECIYVEPDEGEKKG+YWYKNKL
Sbjct: 361 ECIYVEPDEGEKKGIYWYKNKL 382

BLAST of Cucsat.G12630 vs. NCBI nr
Match: XP_038903906.1 (delta(12)-fatty-acid desaturase FAD2 [Benincasa hispida])

HSP 1 Score: 779 bits (2011), Expect = 2.40e-284
Identity = 367/382 (96.07%), Postives = 376/382 (98.43%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRMSSPSSLKK+DSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           TLASIFYYIATTYFQNLPSLLFYPAWA+YWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPSLLFYPAWAVYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS MKWYSTY+NNP+GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPLGRVL 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
            LTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIY+DRERLQIFISDAGLL + YGLY
Sbjct: 181 VLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAIAYGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RLVLAKG AWVVCVYGVPLLIVNGFLVLITYLQHTH ALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVEPDEGEKKGVYWYKNKL 382
           ECIYVEPDEG+K+GV+WYKNKL
Sbjct: 361 ECIYVEPDEGDKRGVFWYKNKL 382

BLAST of Cucsat.G12630 vs. NCBI nr
Match: XP_022934859.1 (delta(12)-fatty-acid desaturase FAD2-like [Cucurbita moschata])

HSP 1 Score: 765 bits (1975), Expect = 7.67e-279
Identity = 362/383 (94.52%), Postives = 373/383 (97.39%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRMSSPSS+KK+DSDHLKRVPSTKPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           TLASIFYYIATTYFQNLP+LLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KS +KWYS Y+NNP+GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
           TLTI LVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLL V+YGLY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTH ALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILG+YYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECIYVEPDEGEK-KGVYWYKNKL 382
           ECIYVEPDEGEK KGV+WY NK 
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNKF 383

BLAST of Cucsat.G12630 vs. NCBI nr
Match: KAG6580810.1 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 763 bits (1970), Expect = 4.43e-278
Identity = 361/383 (94.26%), Postives = 372/383 (97.13%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRMSSPSS+KK+DSDHLKRVPS KPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSAKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           TLASIFYYIATTYFQNLP+LLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KS +KWYS Y+NNP+GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
           TLTI LVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLL V+YGLY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTH ALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILG+YYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECIYVEPDEGEK-KGVYWYKNKL 382
           ECIYVEPDEGEK KGV+WY NK 
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNKF 383

BLAST of Cucsat.G12630 vs. ExPASy TrEMBL
Match: A0A0A0LF25 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G808360 PE=3 SV=1)

HSP 1 Score: 802 bits (2071), Expect = 8.27e-294
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
           TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVEPDEGEKKGVYWYKNKL 382
           ECIYVEPDEGEKKGVYWYKNKL
Sbjct: 361 ECIYVEPDEGEKKGVYWYKNKL 382

BLAST of Cucsat.G12630 vs. ExPASy TrEMBL
Match: A0A5A7TLK3 (Delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005460 PE=3 SV=1)

HSP 1 Score: 787 bits (2032), Expect = 7.30e-288
Identity = 372/382 (97.38%), Postives = 378/382 (98.95%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRMSSPSSLKK+DSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           TLASIFYY+ATTYFQ+LPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS MKWYSTY+NNPVGRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
           TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIY+DRERLQIFISDAGLL V YGLY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RLVLAKG AWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVEPDEGEKKGVYWYKNKL 382
           ECIYVEPDEGEKKG+YWYKNKL
Sbjct: 361 ECIYVEPDEGEKKGIYWYKNKL 382

BLAST of Cucsat.G12630 vs. ExPASy TrEMBL
Match: A0A1S3B762 (delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo OX=3656 GN=LOC103486770 PE=3 SV=1)

HSP 1 Score: 787 bits (2032), Expect = 7.30e-288
Identity = 372/382 (97.38%), Postives = 378/382 (98.95%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRMSSPSSLKK+DSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSLKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           TLASIFYY+ATTYFQ+LPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYVATTYFQSLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKS MKWYSTY+NNPVGRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAMKWYSTYLNNPVGRVL 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
           TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIY+DRERLQIFISDAGLL V YGLY
Sbjct: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYSDRERLQIFISDAGLLAVAYGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RLVLAKG AWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGFAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360

Query: 361 ECIYVEPDEGEKKGVYWYKNKL 382
           ECIYVEPDEGEKKG+YWYKNKL
Sbjct: 361 ECIYVEPDEGEKKGIYWYKNKL 382

BLAST of Cucsat.G12630 vs. ExPASy TrEMBL
Match: A0A6J1F3S1 (delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita moschata OX=3662 GN=LOC111441899 PE=3 SV=1)

HSP 1 Score: 765 bits (1975), Expect = 3.71e-279
Identity = 362/383 (94.52%), Postives = 373/383 (97.39%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRMSSPSS+KK+DSDHLKRVPSTKPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSTKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           TLASIFYYIATTYFQNLP+LLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPNLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KS +KWYS Y+NNP+GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
           TLTI LVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLL V+YGLY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTH ALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILG+YYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECIYVEPDEGEK-KGVYWYKNKL 382
           ECIYVEPDEGEK KGV+WY NK 
Sbjct: 361 ECIYVEPDEGEKTKGVFWYNNKF 383

BLAST of Cucsat.G12630 vs. ExPASy TrEMBL
Match: A0A6J1J0W0 (delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita maxima OX=3661 GN=LOC111481665 PE=3 SV=1)

HSP 1 Score: 762 bits (1967), Expect = 6.15e-278
Identity = 361/383 (94.26%), Postives = 371/383 (96.87%), Query Frame = 0

Query: 1   MGAGGRMSSPSSLKKTDSDHLKRVPSTKPPFTLGELKKAIPPHCFQRSVLRSFSYVVYDL 60
           MGAGGRMSSPSS+KK+DSDHLKRVPS KPPFTLGELKKAIPPHCF RSVLRSFSYVVYDL
Sbjct: 1   MGAGGRMSSPSSIKKSDSDHLKRVPSAKPPFTLGELKKAIPPHCFHRSVLRSFSYVVYDL 60

Query: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
           TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61  TLASIFYYIATTYFQNLPSLLFYPAWALYWAAQGCVLTGVWVLAHECGHHAFSDYQWLDD 120

Query: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSDMKWYSTYINNPVGRVL 180
           TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KS +KWYS Y+NNP+GRVL
Sbjct: 121 TVGLILHSFLMVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSALKWYSPYLNNPLGRVL 180

Query: 181 TLTIQLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLTVTYGLY 240
           TLTI LVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLL V+YGLY
Sbjct: 181 TLTITLVLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERLQIFISDAGLLAVSYGLY 240

Query: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHTALPHYDSSEWDWLRGALATVDRD 300
           RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTH ALPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RLVLAKGLAWVVCVYGVPLLIVNGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 300

Query: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGQYYHFDGTPVVKAMWREAK 360
           YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILG+YYHFDGTP VKAMWREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAK 360

Query: 361 ECIYVEPDEGEK-KGVYWYKNKL 382
           ECIYVEPD GEK KGV+WY NK 
Sbjct: 361 ECIYVEPDAGEKTKGVFWYNNKF 383

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GZC31.6e-19282.46Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV... [more]
P486316.9e-18880.68Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX... [more]
P463132.5e-18578.33Delta(12)-fatty-acid desaturase OS=Arabidopsis thaliana OX=3702 GN=FAD2 PE=1 SV=... [more]
Q392879.4e-18578.91Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea OX=3707 ... [more]
Q84VT21.2e-18477.26Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_004136572.11.71e-293100.00delta(12)-fatty-acid desaturase FAD2 [Cucumis sativus] >KGN59307.1 hypothetical ... [more]
XP_008443062.21.51e-28797.38PREDICTED: delta(12)-fatty-acid desaturase FAD2 [Cucumis melo] >KAA0043728.1 del... [more]
XP_038903906.12.40e-28496.07delta(12)-fatty-acid desaturase FAD2 [Benincasa hispida][more]
XP_022934859.17.67e-27994.52delta(12)-fatty-acid desaturase FAD2-like [Cucurbita moschata][more]
KAG6580810.14.43e-27894.26Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2, partial [Cucurbi... [more]
Match NameE-valueIdentityDescription
A0A0A0LF258.27e-294100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G808360 PE=3 SV=1[more]
A0A5A7TLK37.30e-28897.38Delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3B7627.30e-28897.38delta(12)-fatty-acid desaturase FAD2 OS=Cucumis melo OX=3656 GN=LOC103486770 PE=... [more]
A0A6J1F3S13.71e-27994.52delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1J0W06.15e-27894.26delta(12)-fatty-acid desaturase FAD2-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021863Fatty acid desaturase, N-terminalPFAMPF11960DUF3474coord: 21..61
e-value: 5.4E-8
score: 33.1
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 84..345
e-value: 2.1E-31
score: 109.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availablePANTHERPTHR32100:SF40OLEATE HYDROXYLASE FAH12coord: 1..382
NoneNo IPR availablePANTHERPTHR32100OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTICcoord: 1..382
NoneNo IPR availableCDDcd03507Delta12-FADS-likecoord: 47..329
e-value: 7.29578E-79
score: 240.205

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G12630.T1Cucsat.G12630.T1mRNA
Cucsat.G12630.T4Cucsat.G12630.T4mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006636 unsaturated fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045485 omega-6 fatty acid desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water