Cucsat.G12154 (gene) Cucumber (B10) v3

Overview
NameCucsat.G12154
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionGermin-like protein
Locationctg1837: 1234501 .. 1236292 (-)
RNA-Seq ExpressionCucsat.G12154
SyntenyCucsat.G12154
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAACCCATCTTTAGCTTCTTCCTTAATTTTCCTTCTGCTCTTTGTGTTCTTTTCTTGTCTTGGAATCTCATTAGCAGATTCTAATAATGTCTTTGACACTTGTCCAACTGCCACAAACAGAACTGAGTTCATCAATGGCTACCATTGCAAAAGCCCCTCCCTTGTTACAACTTCTGACTTCAACTCAGCAAAGTTAACGGAACCAGTCAATACCGATGATCGGATCAACTTGGTGGTGAAGATTGTCACTGCTGCAGATTTCCATGGTCTTAACACATTGGGGCTTTCTATTTCTAGAACTGATTTGGTTATAGATGCTATCGTTTCTCCTCACTCGCACCCGAGAGCCACCGAGATGTTCTTTCTGAACCAAGGTACTTAAATAAAAGATTCAATTCATTGTGACCCATCAGGTTGAGTCGTGATCTTACATGCCTTCAGAGTCCAAAGTCCTGTGTCCATTCTCATCTGTTTTTGGGATTTATGATTCACAGGTATTGTTCTAGCTGGATTCATTGACACAAGGAACCAACTGTTTCAAACTATTCTAAAAGCAGGCGATGTAATTGTATTTCCAAAAGGGTTGTTGCACTTCTCTTTGAATTCTGGTTATGAGCCGGCCATAGGTTTCTCTGTGTATAATAGTCAGAATCCAGGGGTCGTAAACGTCAACGGCGCTGTGTTTGATCCTGCTGATTCGAGTCATTTCATAGAAAAGTTCCTTAAGAGACTAAAAGTTCCTTCTTCCCAGTCAAATTTCAAATTCAACCCAAATGTAACTCTTTCCAGATACACAAGTTCGTAAAACTATTAACTCAGTTGGAAAGAAAATGCCTCTACATGGATTAGTTCTGCCCAAGATTCGCTTTGTGAGTTTGAAATCTTAACAAACATTCATAATTTAAGATATAGAAATATTATGGTAACAA

Coding sequence (CDS)

AACCCATCTTTAGCTTCTTCCTTAATTTTCCTTCTGCTCTTTGTGTTCTTTTCTTGTCTTGGAATCTCATTAGCAGATTCTAATAATGTCTTTGACACTTGTCCAACTGCCACAAACAGAACTGAGTTCATCAATGGCTACCATTGCAAAAGCCCCTCCCTTGTTACAACTTCTGACTTCAACTCAGCAAAGTTAACGGAACCAGTCAATACCGATGATCGGATCAACTTGGTGGTGAAGATTGTCACTGCTGCAGATTTCCATGGTCTTAACACATTGGGGCTTTCTATTTCTAGAACTGATTTGGTTATAGATGCTATCGTTTCTCCTCACTCGCACCCGAGAGCCACCGAGATGTTCTTTCTGAACCAAGGTATTGTTCTAGCTGGATTCATTGACACAAGGAACCAACTGTTTCAAACTATTCTAAAAGCAGGCGATGTAATTGTATTTCCAAAAGGGTTGTTGCACTTCTCTTTGAATTCTGGTTATGAGCCGGCCATAGGTTTCTCTGTGTATAATAGTCAGAATCCAGGGGTCGTAAACGTCAACGGCGCTGTGTTTGATCCTGCTGATTCGAGTCATTTCATAGAAAAGTTCCTTAAGAGACTAAAAGTTCCTTCTTCCCAGTCAAATTTCAAATTCAACCCAAATGTAACTCTTTCCAGATACACAAGTTCGTAA

Protein sequence

NPSLASSLIFLLLFVFFSCLGISLADSNNVFDTCPTATNRTEFINGYHCKSPSLVTTSDFNSAKLTEPVNTDDRINLVVKIVTAADFHGLNTLGLSISRTDLVIDAIVSPHSHPRATEMFFLNQGIVLAGFIDTRNQLFQTILKAGDVIVFPKGLLHFSLNSGYEPAIGFSVYNSQNPGVVNVNGAVFDPADSSHFIEKFLKRLKVPSSQSNFKFNPNVTLSRYTSS
Homology
[Close]
BLAST of Cucsat.G12154 vs. NCBI nr
Match: XP_008462780.1 (PREDICTED: disease resistance protein RGA2-like [Cucumis melo])

HSP 1 Score: 152 bits (385), Expect = 1.95e-40
Identity = 71/98 (72.45%), Postives = 86/98 (87.76%), Query Frame = 0 

Query: 1   MINLEQWKEVTTNNASSNVTIFPNLRCLKVNKCPELLNIPKVFHENDVQHLESLTVSFCN 60
           MINL+QW+EV TN+ASSN+TIFPNLR L++ +CP+LLNIP+VF EN+VQHLESL VS CN
Sbjct: 824 MINLQQWEEVMTNDASSNITIFPNLRSLEICRCPKLLNIPEVFDENNVQHLESLIVSHCN 883

Query: 61  KLTKLPKGLHFSSSIRRVKIDCCLNLRVDVRNKSELLF 98
           KLTKLP GLHF SSI+RVKID C NL +++RNKSEL +
Sbjct: 884 KLTKLPNGLHFCSSIQRVKIDQCSNLSINIRNKSELCY 921

[Close]
BLAST of Cucsat.G12154 vs. NCBI nr
Match: AAT77098.1 (Fom-2 protein, partial [Cucumis melo])

HSP 1 Score: 142 bits (357), Expect = 3.09e-37
Identity = 67/98 (68.37%), Postives = 83/98 (84.69%), Query Frame = 0 

Query: 1   MINLEQWKEVTTNNASSNVTIFPNLRCLKVNKCPELLNIPKVFHENDVQHLESLTVSFCN 60
           M+NL+QW+EV TN+ASSN+TIFPNLR L++ +CP+LLNIP+VF EN+VQHLESL VS CN
Sbjct: 300 MVNLQQWEEVMTNDASSNITIFPNLRSLEIRRCPKLLNIPEVFDENNVQHLESLIVSHCN 359

Query: 61  KLTKLPKGLHFSSSIRRVKIDCCLNLRVDVRNKSELLF 98
           KLTKLP GLHF SSI+ VK   C NL +++RNKSEL +
Sbjct: 360 KLTKLPNGLHFCSSIQCVK---CSNLSINIRNKSELCY 394

[Close]
BLAST of Cucsat.G12154 vs. NCBI nr
Match: XP_016902928.1 (PREDICTED: disease resistance protein RGA2-like [Cucumis melo])

HSP 1 Score: 141 bits (355), Expect = 1.86e-36
Identity = 68/98 (69.39%), Postives = 81/98 (82.65%), Query Frame = 0 

Query: 1   MINLEQWKEVTTNNASSNVTIFPNLRCLKVNKCPELLNIPKVFHENDVQHLESLTVSFCN 60
           MINLEQW+EV TN+ASSNVTIFPNLR LK ++CP+LLNIP+VF E+D+QH ESL VS CN
Sbjct: 528 MINLEQWEEVMTNDASSNVTIFPNLRSLKTSRCPKLLNIPEVFDESDMQHFESLFVSHCN 587

Query: 61  KLTKLPKGLHFSSSIRRVKIDCCLNLRVDVRNKSELLF 98
           KLT+LP  L F SSIRRV ID C NL +++RNK EL +
Sbjct: 588 KLTQLPNELQFCSSIRRVTIDHCSNLSINMRNKLELWY 625

[Close]
BLAST of Cucsat.G12154 vs. NCBI nr
Match: XP_004149742.2 (disease resistance protein RGA2 [Cucumis sativus] >KGN58559.1 hypothetical protein Csa_001108 [Cucumis sativus])

HSP 1 Score: 139 bits (349), Expect = 1.41e-35
Identity = 66/98 (67.35%), Postives = 79/98 (80.61%), Query Frame = 0 

Query: 1   MINLEQWKEVTTNNASSNVTIFPNLRCLKVNKCPELLNIPKVFHENDVQHLESLTVSFCN 60
           MINLEQWKEV TN+ SSNVTIFPNL+CLK+  CP+LLNIPK F EN++QHLESL +S CN
Sbjct: 833 MINLEQWKEVITNDESSNVTIFPNLKCLKIWGCPKLLNIPKAFDENNMQHLESLILSCCN 892

Query: 61  KLTKLPKGLHFSSSIRRVKIDCCLNLRVDVRNKSELLF 98
           KLTKLP GL F SSI  + ID C NL +++RNK +L +
Sbjct: 893 KLTKLPDGLQFCSSIEGLTIDKCSNLSINMRNKPKLWY 930

[Close]
BLAST of Cucsat.G12154 vs. NCBI nr
Match: XP_022148515.1 (disease resistance protein RGA2-like [Momordica charantia])

HSP 1 Score: 94.0 bits (232), Expect = 8.71e-20
Identity = 49/99 (49.49%), Postives = 65/99 (65.66%), Query Frame = 0 

Query: 1   MINLEQWKEVTTNNASSNVTIFPNLRCLKVNKCPELLNIPKVF---HENDVQHLESLTVS 60
           M NLEQWKEVT    +SN+T FP L  L + +CP+L+NIP VF    EN V+HL  +T+S
Sbjct: 833 MENLEQWKEVTV---ASNLTTFPCLESLNIWRCPKLMNIPDVFGYCDENGVEHLRHVTIS 892

Query: 61  FCNKLTKLPKGLHFSSSIRRVKIDCCLNLRVDVRNKSEL 96
            C+ L KLP GL F  SI  + I+ C NL + ++NK+ L
Sbjct: 893 GCDGLNKLPNGLQFCHSIEDLTIEDCSNLTLSLQNKNRL 928

[Close]
BLAST of Cucsat.G12154 vs. ExPASy TrEMBL
Match: A0A1S3CHQ5 (disease resistance protein RGA2-like OS=Cucumis melo OX=3656 GN=LOC103501070 PE=4 SV=1)

HSP 1 Score: 152 bits (385), Expect = 9.44e-41
Identity = 71/98 (72.45%), Postives = 86/98 (87.76%), Query Frame = 0 

Query: 1   MINLEQWKEVTTNNASSNVTIFPNLRCLKVNKCPELLNIPKVFHENDVQHLESLTVSFCN 60
           MINL+QW+EV TN+ASSN+TIFPNLR L++ +CP+LLNIP+VF EN+VQHLESL VS CN
Sbjct: 824 MINLQQWEEVMTNDASSNITIFPNLRSLEICRCPKLLNIPEVFDENNVQHLESLIVSHCN 883

Query: 61  KLTKLPKGLHFSSSIRRVKIDCCLNLRVDVRNKSELLF 98
           KLTKLP GLHF SSI+RVKID C NL +++RNKSEL +
Sbjct: 884 KLTKLPNGLHFCSSIQRVKIDQCSNLSINIRNKSELCY 921

[Close]
BLAST of Cucsat.G12154 vs. ExPASy TrEMBL
Match: Q694W1 (Fom-2 protein (Fragment) OS=Cucumis melo OX=3656 GN=Fom-2 PE=4 SV=1)

HSP 1 Score: 142 bits (357), Expect = 1.50e-37
Identity = 67/98 (68.37%), Postives = 83/98 (84.69%), Query Frame = 0 

Query: 1   MINLEQWKEVTTNNASSNVTIFPNLRCLKVNKCPELLNIPKVFHENDVQHLESLTVSFCN 60
           M+NL+QW+EV TN+ASSN+TIFPNLR L++ +CP+LLNIP+VF EN+VQHLESL VS CN
Sbjct: 300 MVNLQQWEEVMTNDASSNITIFPNLRSLEIRRCPKLLNIPEVFDENNVQHLESLIVSHCN 359

Query: 61  KLTKLPKGLHFSSSIRRVKIDCCLNLRVDVRNKSELLF 98
           KLTKLP GLHF SSI+ VK   C NL +++RNKSEL +
Sbjct: 360 KLTKLPNGLHFCSSIQCVK---CSNLSINIRNKSELCY 394

[Close]
BLAST of Cucsat.G12154 vs. ExPASy TrEMBL
Match: A0A1S4E3X3 (disease resistance protein RGA2-like OS=Cucumis melo OX=3656 GN=LOC103501129 PE=4 SV=1)

HSP 1 Score: 141 bits (355), Expect = 8.99e-37
Identity = 68/98 (69.39%), Postives = 81/98 (82.65%), Query Frame = 0 

Query: 1   MINLEQWKEVTTNNASSNVTIFPNLRCLKVNKCPELLNIPKVFHENDVQHLESLTVSFCN 60
           MINLEQW+EV TN+ASSNVTIFPNLR LK ++CP+LLNIP+VF E+D+QH ESL VS CN
Sbjct: 528 MINLEQWEEVMTNDASSNVTIFPNLRSLKTSRCPKLLNIPEVFDESDMQHFESLFVSHCN 587

Query: 61  KLTKLPKGLHFSSSIRRVKIDCCLNLRVDVRNKSELLF 98
           KLT+LP  L F SSIRRV ID C NL +++RNK EL +
Sbjct: 588 KLTQLPNELQFCSSIRRVTIDHCSNLSINMRNKLELWY 625

[Close]
BLAST of Cucsat.G12154 vs. ExPASy TrEMBL
Match: A0A0A0LCB5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G684170 PE=4 SV=1)

HSP 1 Score: 139 bits (349), Expect = 6.81e-36
Identity = 66/98 (67.35%), Postives = 79/98 (80.61%), Query Frame = 0 

Query: 1   MINLEQWKEVTTNNASSNVTIFPNLRCLKVNKCPELLNIPKVFHENDVQHLESLTVSFCN 60
           MINLEQWKEV TN+ SSNVTIFPNL+CLK+  CP+LLNIPK F EN++QHLESL +S CN
Sbjct: 833 MINLEQWKEVITNDESSNVTIFPNLKCLKIWGCPKLLNIPKAFDENNMQHLESLILSCCN 892

Query: 61  KLTKLPKGLHFSSSIRRVKIDCCLNLRVDVRNKSELLF 98
           KLTKLP GL F SSI  + ID C NL +++RNK +L +
Sbjct: 893 KLTKLPDGLQFCSSIEGLTIDKCSNLSINMRNKPKLWY 930

[Close]
BLAST of Cucsat.G12154 vs. ExPASy TrEMBL
Match: A0A6J1D340 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111017143 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 4.22e-20
Identity = 49/99 (49.49%), Postives = 65/99 (65.66%), Query Frame = 0 

Query: 1   MINLEQWKEVTTNNASSNVTIFPNLRCLKVNKCPELLNIPKVF---HENDVQHLESLTVS 60
           M NLEQWKEVT    +SN+T FP L  L + +CP+L+NIP VF    EN V+HL  +T+S
Sbjct: 833 MENLEQWKEVTV---ASNLTTFPCLESLNIWRCPKLMNIPDVFGYCDENGVEHLRHVTIS 892

Query: 61  FCNKLTKLPKGLHFSSSIRRVKIDCCLNLRVDVRNKSEL 96
            C+ L KLP GL F  SI  + I+ C NL + ++NK+ L
Sbjct: 893 GCDGLNKLPNGLQFCHSIEDLTIEDCSNLTLSLQNKNRL 928

The following BLAST results are available for this feature:
BLAST of Cucsat.G12154 vs.
Match NameE-valueIdentityDescription
BLAST of Cucsat.G12154 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008462780.11.95e-4072.45PREDICTED: disease resistance protein RGA2-like [Cucumis melo][more]
AAT77098.13.09e-3768.37Fom-2 protein, partial [Cucumis melo][more]
XP_016902928.11.86e-3669.39PREDICTED: disease resistance protein RGA2-like [Cucumis melo][more]
XP_004149742.21.41e-3567.35disease resistance protein RGA2 [Cucumis sativus] >KGN58559.1 hypothetical prote... [more]
XP_022148515.18.71e-2049.49disease resistance protein RGA2-like [Momordica charantia][more]
BLAST of Cucsat.G12154 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3CHQ59.44e-4172.45disease resistance protein RGA2-like OS=Cucumis melo OX=3656 GN=LOC103501070 PE=... [more]
Q694W11.50e-3768.37Fom-2 protein (Fragment) OS=Cucumis melo OX=3656 GN=Fom-2 PE=4 SV=1[more]
A0A1S4E3X38.99e-3769.39disease resistance protein RGA2-like OS=Cucumis melo OX=3656 GN=LOC103501129 PE=... [more]
A0A0A0LCB56.81e-3667.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G684170 PE=4 SV=1[more]
A0A6J1D3404.22e-2049.49disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111017... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001929GerminPRINTSPR00325GERMINcoord: 23..43
score: 52.16
coord: 56..71
score: 42.59
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 2..69
e-value: 5.3E-13
score: 48.8
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 1..98
e-value: 5.0E-22
score: 80.3
NoneNo IPR availablePANTHERPTHR31238GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3coord: 1..89
NoneNo IPR availablePANTHERPTHR31238:SF3GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 4coord: 1..89
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 2..67

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G12154.T3Cucsat.G12154.T3mRNA
Cucsat.G12154.T4Cucsat.G12154.T4mRNA
Cucsat.G12154.T2Cucsat.G12154.T2mRNA
Cucsat.G12154.T1Cucsat.G12154.T1mRNA
Cucsat.G12154.T5Cucsat.G12154.T5mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
cellular_component GO:0048046 apoplast
molecular_function GO:0043531 ADP binding
molecular_function GO:0030145 manganese ion binding