Cucsat.G12000 (gene) Cucumber (B10) v3

Overview
NameCucsat.G12000
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionGDP-mannose 4,6-dehydratase
Locationctg1820: 4795989 .. 4798314 (-)
RNA-Seq ExpressionCucsat.G12000
SyntenyCucsat.G12000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTTACGCGGCTACTATAATTAGAGCTTTCACCCTTCTCTTTCTTCTTCACATTTCTCTCTTTCTCTTCTCATTTCTTCCAGATCCGATTCATCCATCAATGGCTTCCATCGCCGACGGTACCAGATCTACCTCCGATACCAATGGCAAGGCCGCTGAACCGGCGGAGAAGCGACGGACGGCCTTAATTACTGGCATTACTGGTCAGGATGGTTCCTACCTTACTGAGTTCCTTCTCGATAAGGGCTATGAAGTCCATGGATTGATTCGGAGATCTTCCAACTTCAATACTCAACGAATCAACCACATCTATATCGATCCTCACAATGCTAATAAGGCACGAATGAAGCTTCACTATGCTGACCTTACTGACGCCTCCTCTCTCCGTCGATGGCTTGATATTATTCTTCCTGATGAGGTTTATAACCTCGCAGCTCAATCTCATGTTGCTGTTTCTTTCGAAATTCCGGATTATACTGCTGATGTTGTTGCCACTGGTGCTCTACGTTTGCTTGAGGCTGTTCGATCTCATATCGCTGCTACTGGTCGTAGCCACATTCGATATTACCAAGCGGGATCGTCTGAAATGTTTGGGTCTACGCCGCCTCCTCAGTCGGAGACGACTCCATTTCATCCTCGATCTCCTTATGCTGCAGCGAAATGCGCTGCGCATTGGTATACTGTTAATTACCGTGAGGCCTACGGGATTTTCGCTTGTAATGGGATACTATTCAACCATGAATCGCCTCGCAGGGGAGAGAATTTCGTGACGAGGAAGATCACACGAGCTGTAGGTCGGATCAAGATTGGACTGCAGAGCAAGCTGTTCTTGGGGAATCTGCAGGCGTCGAGGGATTGGGGTTTTGCAGGGGATTATGTGGAAGCGATGTGGATGATGCTGCAGCAAGATCAACCTGATGATTACGTGGTTGCGACGGAGGAATCTCATACGGTGAAGGAGTTTTTGGAGGTGGCATTTGGGTATGTTGGATTGAATTGGAAAGATCATGTAGTGATCGACAAGAGATACTTCCGGCCGGCGGAGGTTGATAATCTGAAGGGTGATGCAAGCAAGGTGAAAAGGGTATTGGGATGGAAACCAAAAGTTGGATTTGAGAAGCTGGTGAAGATGATGGTGGATGAAGACGTTGAAATAGCCAAGCGAGAGAAGGTATTGGTTGACGCTGGCTATATGGATGCACAGCAACAGCCTTGATTGCTTCAGATCAGATAGAACCAACCAACCAACAACCACTGTACGCTGCAAATTCCCCGATCCTAATGTTCTTCAAATGCAACCTTATTGAGTAGAGTAATATAGTTTTGATTTGCAGAATTCATTGTCCCTGCTTTTCTTTGTGTTATTTAATCTTATATAATGTTCTTTCACAATCAGTTGTTGCATCTGTTCTCAACTCAACATGTGTTCACAAAAATGTTATTAATAAATACACTACACTACTTCTTCTTTTTTCCCATCTCTGTTCTTCTCATGAATCCAAAATTCGTGATTGCATTATTTTTAAATGGAGTTTCTGACAAATCAACCTTTAAAAACACTTAAAAATTCAGGCTATAGCTTAGGTGAAAGCACCGACAGTGCCATCTTACAATCAATGTGATTTATCGAAATGTCACATGGTGTGTTCAGGCTCTAGAAGATGGATCTTAACAAGCAAAACGAGAAGGTGACATCGGTGGGGAACTTCCAGGGGATGAAAGGGCTAGAGAGGAGAAGTTCGTAATGTGGCTGTTGCCTCTGCCCTGGGCAGCTGGTTGGTTGGTCTGCCGGTGGCGATTTTAGTGGGAGCCAACTCCTTTCAAAGGGCTTCTACGGTCAGCGGTCCAGATTTTGATTTTGGAACACTGCTTTATGACATTTTCCTGACAGCTTGATGTTGCTGTAAACAGCAAATTTGACAATGGATGAATGCTGGAAAAAGAAACCAACGAATCAAGAATTGGCATTTTTATATTTACCATCTTTTGCAATGGAGTGCTTTTGTTTCCTAAATTACAAAACGATTTCTAATAATGAATCAATTTAATTTGGGTTCTGGGGGATGTGGATCTGTATTCAAAGCTACTTGTTCAGAAGATGAGTTGCAGGATGACCCTGTGTCGGCCGTTGCAAGAACAAATGGCTTGATAGATGACCGAAGAGACTCCGGACCCTCTGAGGCAATTCTTCTGCATAATGCATCGCAATTACTAATAACCTCTGTCAATCTTCCCTTGAGCTCACCGATTTCCACTTCCAGTGAGTGAACTACATCACACACATCCAAAGGCAGACAATAGTACATTTTCTTTGTAGAACACTAAC

Coding sequence (CDS)

CTTTACGCGGCTACTATAATTAGAGCTTTCACCCTTCTCTTTCTTCTTCACATTTCTCTCTTTCTCTTCTCATTTCTTCCAGATCCGATTCATCCATCAATGGCTTCCATCGCCGACGGTACCAGATCTACCTCCGATACCAATGGCAAGGCCGCTGAACCGGCGGAGAAGCGACGGACGGCCTTAATTACTGGCATTACTGGTCAGGATGGTTCCTACCTTACTGAGTTCCTTCTCGATAAGGGCTATGAAGTCCATGGATTGATTCGGAGATCTTCCAACTTCAATACTCAACGAATCAACCACATCTATATCGATCCTCACAATGCTAATAAGGCACGAATGAAGCTTCACTATGCTGACCTTACTGACGCCTCCTCTCTCCGTCGATGGCTTGATATTATTCTTCCTGATGAGGTTTATAACCTCGCAGCTCAATCTCATGTTGCTGTTTCTTTCGAAATTCCGGATTATACTGCTGATGTTGTTGCCACTGGTGCTCTACGTTTGCTTGAGGCTGTTCGATCTCATATCGCTGCTACTGGTCGTAGCCACATTCGATATTACCAAGCGGGATCGTCTGAAATGTTTGGGTCTACGCCGCCTCCTCAGTCGGAGACGACTCCATTTCATCCTCGATCTCCTTATGCTGCAGCGAAATGCGCTGCGCATTGGTATACTGTTAATTACCGTGAGGCCTACGGGATTTTCGCTTGTAATGGGATACTATTCAACCATGAATCGCCTCGCAGGGGAGAGAATTTCGTGACGAGGAAGATCACACGAGCTGTAGGTCGGATCAAGATTGGACTGCAGAGCAAGCTGTTCTTGGGGAATCTGCAGGCGTCGAGGGATTGGGGTTTTGCAGGGGATTATGTGGAAGCGATGTGGATGATGCTGCAGCAAGATCAACCTGATGATTACGTGGTTGCGACGGAGGAATCTCATACGGTGAAGGAGTTTTTGGAGGTGGCATTTGGGTATGTTGGATTGAATTGGAAAGATCATGTAGTGATCGACAAGAGATACTTCCGGCCGGCGGAGGTTGATAATCTGAAGGGTGATGCAAGCAAGGTGAAAAGGGTATTGGGATGGAAACCAAAAGTTGGATTTGAGAAGCTGGTGAAGATGATGGTGGATGAAGACGTTGAAATAGCCAAGCGAGAGAAGGTATTGGTTGACGCTGGCTATATGGATGCACAGCAACAGCCTTGA

Protein sequence

LYAATIIRAFTLLFLLHISLFLFSFLPDPIHPSMASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSSNFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPRSPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNWKDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLVDAGYMDAQQQP
Homology
BLAST of Cucsat.G12000 vs. ExPASy Swiss-Prot
Match: P93031 (GDP-mannose 4,6 dehydratase 2 OS=Arabidopsis thaliana OX=3702 GN=MUR1 PE=1 SV=3)

HSP 1 Score: 646.4 bits (1666), Expect = 2.2e-184
Identity = 319/374 (85.29%), Postives = 349/374 (93.32%), Query Frame = 0

Query: 34  MASIADGTRSTSD--TNGKA-AEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIR 93
           MAS  +G+RS S+  T  KA +   E R+ ALITGITGQDGSYLTEFLL KGYEVHGLIR
Sbjct: 1   MASENNGSRSDSESITAPKADSTVVEPRKIALITGITGQDGSYLTEFLLGKGYEVHGLIR 60

Query: 94  RSSNFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVA 153
           RSSNFNTQRINHIYIDPHN NKA MKLHYADLTDASSLRRW+D+I PDEVYNLAAQSHVA
Sbjct: 61  RSSNFNTQRINHIYIDPHNVNKALMKLHYADLTDASSLRRWIDVIKPDEVYNLAAQSHVA 120

Query: 154 VSFEIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPF 213
           VSFEIPDYTADVVATGALRLLEAVRSH   +GR+ ++YYQAGSSEMFGSTPPPQSETTPF
Sbjct: 121 VSFEIPDYTADVVATGALRLLEAVRSHTIDSGRT-VKYYQAGSSEMFGSTPPPQSETTPF 180

Query: 214 HPRSPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIG 273
           HPRSPYAA+KCAAHWYTVNYREAYG+FACNGILFNHESPRRGENFVTRKITRA+GRIK+G
Sbjct: 181 HPRSPYAASKCAAHWYTVNYREAYGLFACNGILFNHESPRRGENFVTRKITRALGRIKVG 240

Query: 274 LQSKLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVG 333
           LQ+KLFLGNLQASRDWGFAGDYVEAMW+MLQQ++PDDYVVATEE HTV+EFL+V+FGY+G
Sbjct: 241 LQTKLFLGNLQASRDWGFAGDYVEAMWLMLQQEKPDDYVVATEEGHTVEEFLDVSFGYLG 300

Query: 334 LNWKDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREK 393
           LNWKD+V ID+RYFRPAEVDNL+GDASK K VLGWKP+VGFEKLVKMMVDED+E+AKREK
Sbjct: 301 LNWKDYVEIDQRYFRPAEVDNLQGDASKAKEVLGWKPQVGFEKLVKMMVDEDLELAKREK 360

Query: 394 VLVDAGYMDAQQQP 405
           VLVDAGYMDA+QQP
Sbjct: 361 VLVDAGYMDAKQQP 373

BLAST of Cucsat.G12000 vs. ExPASy Swiss-Prot
Match: Q9SNY3 (GDP-mannose 4,6 dehydratase 1 OS=Arabidopsis thaliana OX=3702 GN=GMD1 PE=1 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 6.4e-184
Identity = 308/347 (88.76%), Postives = 332/347 (95.68%), Query Frame = 0

Query: 58  RRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSSNFNTQRINHIYIDPHNANKARMKL 117
           R+ AL+TGITGQDGSYLTEFLL+KGYEVHGLIRRSSNFNTQR+NHIY+DPHN NKA MKL
Sbjct: 16  RKIALVTGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQRLNHIYVDPHNVNKALMKL 75

Query: 118 HYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSH 177
           HY DL+DASSLRRWLD+I PDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSH
Sbjct: 76  HYGDLSDASSLRRWLDVIKPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSH 135

Query: 178 IAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPRSPYAAAKCAAHWYTVNYREAYGIF 237
               GR+ I+YYQAGSSEMFGSTPPPQSETTPFHPRSPYAA+KCAAHWYTVNYREAYG++
Sbjct: 136 NIDNGRA-IKYYQAGSSEMFGSTPPPQSETTPFHPRSPYAASKCAAHWYTVNYREAYGLY 195

Query: 238 ACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFLGNLQASRDWGFAGDYVEAMW 297
           ACNGILFNHESPRRGENFVTRKITRA+GRIK+GLQ+KLFLGN+QASRDWGFAGDYVEAMW
Sbjct: 196 ACNGILFNHESPRRGENFVTRKITRALGRIKVGLQTKLFLGNIQASRDWGFAGDYVEAMW 255

Query: 298 MMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNWKDHVVIDKRYFRPAEVDNLKGDAS 357
           +MLQQ++PDDYVVATEESHTVKEFL+V+FGYVGLNWKDHV IDKRYFRP EVDNLKGDAS
Sbjct: 256 LMLQQEKPDDYVVATEESHTVKEFLDVSFGYVGLNWKDHVEIDKRYFRPTEVDNLKGDAS 315

Query: 358 KVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLVDAGYMDAQQQP 405
           K K +LGWKPKVGFEKLVKMMVDED+E+AKREKVL DAGYMDAQQQP
Sbjct: 316 KAKEMLGWKPKVGFEKLVKMMVDEDLELAKREKVLADAGYMDAQQQP 361

BLAST of Cucsat.G12000 vs. ExPASy Swiss-Prot
Match: P0AC90 (GDP-mannose 4,6-dehydratase OS=Escherichia coli O157:H7 OX=83334 GN=gmd PE=3 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.9e-106
Identity = 197/367 (53.68%), Postives = 251/367 (68.39%), Query Frame = 0

Query: 59  RTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSSNFNTQRINHIYIDPHNANKARMKLH 118
           + ALITG+TGQDGSYL EFLL+KGYEVHG+ RR+S+FNT+R++HIY DPH  N  +  LH
Sbjct: 3   KVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDPHTCN-PKFHLH 62

Query: 119 YADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSHI 178
           Y DL+D S+L R L  + PDEVYNL A SHVAVSFE P+YTADV A G LRLLEA+R   
Sbjct: 63  YGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYTADVDAMGTLRLLEAIR--- 122

Query: 179 AATGRSHIRYYQAGSSEMFGSTPP-PQSETTPFHPRSPYAAAKCAAHWYTVNYREAYGIF 238
                   R+YQA +SE++G     PQ ETTPF+PRSPYA AK  A+W TVNYRE+YG++
Sbjct: 123 FLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGMY 182

Query: 239 ACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFLGNLQASRDWGFAGDYVEAMW 298
           ACNGILFNHESPRRGE FVTRKITRA+  I  GL+S L+LGN+ + RDWG A DYV+  W
Sbjct: 183 ACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVKMQW 242

Query: 299 MMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNWKDH--------------------- 358
           MMLQQ+QP+D+V+AT   ++V++F+E+A   +G+  +                       
Sbjct: 243 MMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRFEGTGVEEKGIVVSVTGHDAPGV 302

Query: 359 ------VVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREK 398
                 + +D RYFRPAEV+ L GD +K    LGWKP++   ++V  MV  D+E AK+  
Sbjct: 303 KPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWKPEITLREMVSEMVANDLEAAKKHS 362

BLAST of Cucsat.G12000 vs. ExPASy Swiss-Prot
Match: P0AC89 (GDP-mannose 4,6-dehydratase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=gmd PE=3 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.9e-106
Identity = 197/367 (53.68%), Postives = 251/367 (68.39%), Query Frame = 0

Query: 59  RTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSSNFNTQRINHIYIDPHNANKARMKLH 118
           + ALITG+TGQDGSYL EFLL+KGYEVHG+ RR+S+FNT+R++HIY DPH  N  +  LH
Sbjct: 3   KVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDPHTCN-PKFHLH 62

Query: 119 YADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSHI 178
           Y DL+D S+L R L  + PDEVYNL A SHVAVSFE P+YTADV A G LRLLEA+R   
Sbjct: 63  YGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYTADVDAMGTLRLLEAIR--- 122

Query: 179 AATGRSHIRYYQAGSSEMFGSTPP-PQSETTPFHPRSPYAAAKCAAHWYTVNYREAYGIF 238
                   R+YQA +SE++G     PQ ETTPF+PRSPYA AK  A+W TVNYRE+YG++
Sbjct: 123 FLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGMY 182

Query: 239 ACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFLGNLQASRDWGFAGDYVEAMW 298
           ACNGILFNHESPRRGE FVTRKITRA+  I  GL+S L+LGN+ + RDWG A DYV+  W
Sbjct: 183 ACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVKMQW 242

Query: 299 MMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNWKDH--------------------- 358
           MMLQQ+QP+D+V+AT   ++V++F+E+A   +G+  +                       
Sbjct: 243 MMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRFEGTGVEEKGIVVSVTGHDAPGV 302

Query: 359 ------VVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREK 398
                 + +D RYFRPAEV+ L GD +K    LGWKP++   ++V  MV  D+E AK+  
Sbjct: 303 KPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWKPEITLREMVSEMVANDLEAAKKHS 362

BLAST of Cucsat.G12000 vs. ExPASy Swiss-Prot
Match: P0AC88 (GDP-mannose 4,6-dehydratase OS=Escherichia coli (strain K12) OX=83333 GN=gmd PE=1 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.9e-106
Identity = 197/367 (53.68%), Postives = 251/367 (68.39%), Query Frame = 0

Query: 59  RTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSSNFNTQRINHIYIDPHNANKARMKLH 118
           + ALITG+TGQDGSYL EFLL+KGYEVHG+ RR+S+FNT+R++HIY DPH  N  +  LH
Sbjct: 3   KVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDPHTCN-PKFHLH 62

Query: 119 YADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSHI 178
           Y DL+D S+L R L  + PDEVYNL A SHVAVSFE P+YTADV A G LRLLEA+R   
Sbjct: 63  YGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYTADVDAMGTLRLLEAIR--- 122

Query: 179 AATGRSHIRYYQAGSSEMFGSTPP-PQSETTPFHPRSPYAAAKCAAHWYTVNYREAYGIF 238
                   R+YQA +SE++G     PQ ETTPF+PRSPYA AK  A+W TVNYRE+YG++
Sbjct: 123 FLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGMY 182

Query: 239 ACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFLGNLQASRDWGFAGDYVEAMW 298
           ACNGILFNHESPRRGE FVTRKITRA+  I  GL+S L+LGN+ + RDWG A DYV+  W
Sbjct: 183 ACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVKMQW 242

Query: 299 MMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNWKDH--------------------- 358
           MMLQQ+QP+D+V+AT   ++V++F+E+A   +G+  +                       
Sbjct: 243 MMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRFEGTGVEEKGIVVSVTGHDAPGV 302

Query: 359 ------VVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREK 398
                 + +D RYFRPAEV+ L GD +K    LGWKP++   ++V  MV  D+E AK+  
Sbjct: 303 KPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWKPEITLREMVSEMVANDLEAAKKHS 362

BLAST of Cucsat.G12000 vs. NCBI nr
Match: XP_011653164.1 (GDP-mannose 4,6 dehydratase 1 [Cucumis sativus] >KGN53317.1 hypothetical protein Csa_015297 [Cucumis sativus])

HSP 1 Score: 744 bits (1921), Expect = 2.01e-270
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 34  MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 93
           MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS
Sbjct: 1   MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 60

Query: 94  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 153
           NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF
Sbjct: 61  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 120

Query: 154 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 213
           EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR
Sbjct: 121 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 180

Query: 214 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 273
           SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS
Sbjct: 181 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 240

Query: 274 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 333
           KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW
Sbjct: 241 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 300

Query: 334 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 393
           KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV
Sbjct: 301 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 360

Query: 394 DAGYMDAQQQP 404
           DAGYMDAQQQP
Sbjct: 361 DAGYMDAQQQP 371

BLAST of Cucsat.G12000 vs. NCBI nr
Match: XP_008452068.1 (PREDICTED: GDP-mannose 4,6 dehydratase 1 [Cucumis melo] >KAA0044808.1 GDP-mannose 4,6 dehydratase 1 [Cucumis melo var. makuwa] >TYK16656.1 GDP-mannose 4,6 dehydratase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 743 bits (1917), Expect = 8.19e-270
Identity = 370/371 (99.73%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 34  MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 93
           MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS
Sbjct: 1   MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 60

Query: 94  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 153
           NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF
Sbjct: 61  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 120

Query: 154 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 213
           EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR
Sbjct: 121 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 180

Query: 214 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 273
           SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS
Sbjct: 181 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 240

Query: 274 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 333
           KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTV+EFLEVAFGYVGLNW
Sbjct: 241 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVEEFLEVAFGYVGLNW 300

Query: 334 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 393
           KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV
Sbjct: 301 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 360

Query: 394 DAGYMDAQQQP 404
           DAGYMDAQQQP
Sbjct: 361 DAGYMDAQQQP 371

BLAST of Cucsat.G12000 vs. NCBI nr
Match: XP_038891859.1 (GDP-mannose 4,6 dehydratase 1 [Benincasa hispida])

HSP 1 Score: 733 bits (1893), Expect = 3.72e-266
Identity = 363/371 (97.84%), Postives = 370/371 (99.73%), Query Frame = 0

Query: 34  MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 93
           MASIADGTRS SDTNGKAA+PAEKRRTALITGITGQDGSYLTEFLL+KGYEVHGLIRRSS
Sbjct: 1   MASIADGTRSASDTNGKAADPAEKRRTALITGITGQDGSYLTEFLLEKGYEVHGLIRRSS 60

Query: 94  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 153
           NFNTQRINHIYIDPHNA+KARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF
Sbjct: 61  NFNTQRINHIYIDPHNAHKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 120

Query: 154 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 213
           EIPDYTADVVATGALRLLEAVRSHI+ATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR
Sbjct: 121 EIPDYTADVVATGALRLLEAVRSHISATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 180

Query: 214 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 273
           SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS
Sbjct: 181 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 240

Query: 274 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 333
           KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTV+EFLEVAFGYVGLNW
Sbjct: 241 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVEEFLEVAFGYVGLNW 300

Query: 334 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 393
           KDHVVIDKRYFRPAEVDNLKGDASKVK+VLGWKPKVGFEKLVKMMVDED+EIAKREKVLV
Sbjct: 301 KDHVVIDKRYFRPAEVDNLKGDASKVKKVLGWKPKVGFEKLVKMMVDEDIEIAKREKVLV 360

Query: 394 DAGYMDAQQQP 404
           DAGYMDAQQQP
Sbjct: 361 DAGYMDAQQQP 371

BLAST of Cucsat.G12000 vs. NCBI nr
Match: XP_022942737.1 (GDP-mannose 4,6 dehydratase 1-like [Cucurbita moschata] >XP_023548256.1 GDP-mannose 4,6 dehydratase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 714 bits (1842), Expect = 2.20e-258
Identity = 354/371 (95.42%), Postives = 360/371 (97.04%), Query Frame = 0

Query: 34  MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 93
           MASIADGTRS S+TN +   P EKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS
Sbjct: 1   MASIADGTRSASNTNVEGTAPTEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 60

Query: 94  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 153
           NFNTQRINHIYIDPHN +KARMKLHYADLTDASSLRRWLD ILPDEVYNLAAQSHVAVSF
Sbjct: 61  NFNTQRINHIYIDPHNVHKARMKLHYADLTDASSLRRWLDTILPDEVYNLAAQSHVAVSF 120

Query: 154 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 213
           EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQ ETTPFHPR
Sbjct: 121 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQLETTPFHPR 180

Query: 214 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 273
           SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS
Sbjct: 181 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 240

Query: 274 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 333
           KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTV+EFLEVAFGYVGLNW
Sbjct: 241 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVEEFLEVAFGYVGLNW 300

Query: 334 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 393
           KDHVVIDKRYFRP EVDNLKGDASK K+VLGWKPKVGFEKLVKMMVDEDVE+AKREKVLV
Sbjct: 301 KDHVVIDKRYFRPTEVDNLKGDASKAKKVLGWKPKVGFEKLVKMMVDEDVEMAKREKVLV 360

Query: 394 DAGYMDAQQQP 404
           DAGYMDAQQQP
Sbjct: 361 DAGYMDAQQQP 371

BLAST of Cucsat.G12000 vs. NCBI nr
Match: XP_022981559.1 (GDP-mannose 4,6 dehydratase 1-like [Cucurbita maxima])

HSP 1 Score: 712 bits (1839), Expect = 6.31e-258
Identity = 354/371 (95.42%), Postives = 360/371 (97.04%), Query Frame = 0

Query: 34  MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 93
           MASIADGTRS S+TN +A  P  KRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS
Sbjct: 1   MASIADGTRSASNTNVEATAPTGKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 60

Query: 94  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 153
           NFNTQRINHIYIDPHN +KARMKLHYADLTDASSLRRWLD ILPDEVYNLAAQSHVAVSF
Sbjct: 61  NFNTQRINHIYIDPHNVHKARMKLHYADLTDASSLRRWLDTILPDEVYNLAAQSHVAVSF 120

Query: 154 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 213
           EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQ ETTPFHPR
Sbjct: 121 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQLETTPFHPR 180

Query: 214 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 273
           SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS
Sbjct: 181 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 240

Query: 274 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 333
           KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTV+EFLEVAFGYVGLNW
Sbjct: 241 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVEEFLEVAFGYVGLNW 300

Query: 334 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 393
           KDHVVIDKRYFRP EVDNLKGDASK K+VLGWKPKVGFEKLVKMMVDEDVE+AKREKVLV
Sbjct: 301 KDHVVIDKRYFRPTEVDNLKGDASKAKKVLGWKPKVGFEKLVKMMVDEDVEMAKREKVLV 360

Query: 394 DAGYMDAQQQP 404
           DAGYMDAQQQP
Sbjct: 361 DAGYMDAQQQP 371

BLAST of Cucsat.G12000 vs. ExPASy TrEMBL
Match: A0A0A0KY32 (GDP-mannose 4,6-dehydratase OS=Cucumis sativus OX=3659 GN=Csa_4G046810 PE=3 SV=1)

HSP 1 Score: 744 bits (1921), Expect = 9.74e-271
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 34  MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 93
           MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS
Sbjct: 1   MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 60

Query: 94  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 153
           NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF
Sbjct: 61  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 120

Query: 154 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 213
           EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR
Sbjct: 121 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 180

Query: 214 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 273
           SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS
Sbjct: 181 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 240

Query: 274 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 333
           KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW
Sbjct: 241 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 300

Query: 334 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 393
           KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV
Sbjct: 301 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 360

Query: 394 DAGYMDAQQQP 404
           DAGYMDAQQQP
Sbjct: 361 DAGYMDAQQQP 371

BLAST of Cucsat.G12000 vs. ExPASy TrEMBL
Match: A0A5D3D1T2 (GDP-mannose 4,6-dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004870 PE=3 SV=1)

HSP 1 Score: 743 bits (1917), Expect = 3.96e-270
Identity = 370/371 (99.73%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 34  MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 93
           MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS
Sbjct: 1   MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 60

Query: 94  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 153
           NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF
Sbjct: 61  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 120

Query: 154 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 213
           EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR
Sbjct: 121 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 180

Query: 214 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 273
           SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS
Sbjct: 181 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 240

Query: 274 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 333
           KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTV+EFLEVAFGYVGLNW
Sbjct: 241 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVEEFLEVAFGYVGLNW 300

Query: 334 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 393
           KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV
Sbjct: 301 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 360

Query: 394 DAGYMDAQQQP 404
           DAGYMDAQQQP
Sbjct: 361 DAGYMDAQQQP 371

BLAST of Cucsat.G12000 vs. ExPASy TrEMBL
Match: A0A1S3BT16 (GDP-mannose 4,6-dehydratase OS=Cucumis melo OX=3656 GN=LOC103493184 PE=3 SV=1)

HSP 1 Score: 743 bits (1917), Expect = 3.96e-270
Identity = 370/371 (99.73%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 34  MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 93
           MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS
Sbjct: 1   MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 60

Query: 94  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 153
           NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF
Sbjct: 61  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 120

Query: 154 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 213
           EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR
Sbjct: 121 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 180

Query: 214 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 273
           SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS
Sbjct: 181 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 240

Query: 274 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 333
           KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTV+EFLEVAFGYVGLNW
Sbjct: 241 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVEEFLEVAFGYVGLNW 300

Query: 334 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 393
           KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV
Sbjct: 301 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 360

Query: 394 DAGYMDAQQQP 404
           DAGYMDAQQQP
Sbjct: 361 DAGYMDAQQQP 371

BLAST of Cucsat.G12000 vs. ExPASy TrEMBL
Match: A0A6J1FR39 (GDP-mannose 4,6-dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111447684 PE=3 SV=1)

HSP 1 Score: 714 bits (1842), Expect = 1.07e-258
Identity = 354/371 (95.42%), Postives = 360/371 (97.04%), Query Frame = 0

Query: 34  MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 93
           MASIADGTRS S+TN +   P EKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS
Sbjct: 1   MASIADGTRSASNTNVEGTAPTEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 60

Query: 94  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 153
           NFNTQRINHIYIDPHN +KARMKLHYADLTDASSLRRWLD ILPDEVYNLAAQSHVAVSF
Sbjct: 61  NFNTQRINHIYIDPHNVHKARMKLHYADLTDASSLRRWLDTILPDEVYNLAAQSHVAVSF 120

Query: 154 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 213
           EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQ ETTPFHPR
Sbjct: 121 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQLETTPFHPR 180

Query: 214 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 273
           SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS
Sbjct: 181 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 240

Query: 274 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 333
           KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTV+EFLEVAFGYVGLNW
Sbjct: 241 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVEEFLEVAFGYVGLNW 300

Query: 334 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 393
           KDHVVIDKRYFRP EVDNLKGDASK K+VLGWKPKVGFEKLVKMMVDEDVE+AKREKVLV
Sbjct: 301 KDHVVIDKRYFRPTEVDNLKGDASKAKKVLGWKPKVGFEKLVKMMVDEDVEMAKREKVLV 360

Query: 394 DAGYMDAQQQP 404
           DAGYMDAQQQP
Sbjct: 361 DAGYMDAQQQP 371

BLAST of Cucsat.G12000 vs. ExPASy TrEMBL
Match: A0A6J1J273 (GDP-mannose 4,6-dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111480638 PE=3 SV=1)

HSP 1 Score: 712 bits (1839), Expect = 3.05e-258
Identity = 354/371 (95.42%), Postives = 360/371 (97.04%), Query Frame = 0

Query: 34  MASIADGTRSTSDTNGKAAEPAEKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 93
           MASIADGTRS S+TN +A  P  KRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS
Sbjct: 1   MASIADGTRSASNTNVEATAPTGKRRTALITGITGQDGSYLTEFLLDKGYEVHGLIRRSS 60

Query: 94  NFNTQRINHIYIDPHNANKARMKLHYADLTDASSLRRWLDIILPDEVYNLAAQSHVAVSF 153
           NFNTQRINHIYIDPHN +KARMKLHYADLTDASSLRRWLD ILPDEVYNLAAQSHVAVSF
Sbjct: 61  NFNTQRINHIYIDPHNVHKARMKLHYADLTDASSLRRWLDTILPDEVYNLAAQSHVAVSF 120

Query: 154 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQSETTPFHPR 213
           EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQ ETTPFHPR
Sbjct: 121 EIPDYTADVVATGALRLLEAVRSHIAATGRSHIRYYQAGSSEMFGSTPPPQLETTPFHPR 180

Query: 214 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 273
           SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS
Sbjct: 181 SPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQS 240

Query: 274 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVKEFLEVAFGYVGLNW 333
           KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTV+EFLEVAFGYVGLNW
Sbjct: 241 KLFLGNLQASRDWGFAGDYVEAMWMMLQQDQPDDYVVATEESHTVEEFLEVAFGYVGLNW 300

Query: 334 KDHVVIDKRYFRPAEVDNLKGDASKVKRVLGWKPKVGFEKLVKMMVDEDVEIAKREKVLV 393
           KDHVVIDKRYFRP EVDNLKGDASK K+VLGWKPKVGFEKLVKMMVDEDVE+AKREKVLV
Sbjct: 301 KDHVVIDKRYFRPTEVDNLKGDASKAKKVLGWKPKVGFEKLVKMMVDEDVEMAKREKVLV 360

Query: 394 DAGYMDAQQQP 404
           DAGYMDAQQQP
Sbjct: 361 DAGYMDAQQQP 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P930312.2e-18485.29GDP-mannose 4,6 dehydratase 2 OS=Arabidopsis thaliana OX=3702 GN=MUR1 PE=1 SV=3[more]
Q9SNY36.4e-18488.76GDP-mannose 4,6 dehydratase 1 OS=Arabidopsis thaliana OX=3702 GN=GMD1 PE=1 SV=1[more]
P0AC901.9e-10653.68GDP-mannose 4,6-dehydratase OS=Escherichia coli O157:H7 OX=83334 GN=gmd PE=3 SV=... [more]
P0AC891.9e-10653.68GDP-mannose 4,6-dehydratase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 7009... [more]
P0AC881.9e-10653.68GDP-mannose 4,6-dehydratase OS=Escherichia coli (strain K12) OX=83333 GN=gmd PE=... [more]
Match NameE-valueIdentityDescription
XP_011653164.12.01e-270100.00GDP-mannose 4,6 dehydratase 1 [Cucumis sativus] >KGN53317.1 hypothetical protein... [more]
XP_008452068.18.19e-27099.73PREDICTED: GDP-mannose 4,6 dehydratase 1 [Cucumis melo] >KAA0044808.1 GDP-mannos... [more]
XP_038891859.13.72e-26697.84GDP-mannose 4,6 dehydratase 1 [Benincasa hispida][more]
XP_022942737.12.20e-25895.42GDP-mannose 4,6 dehydratase 1-like [Cucurbita moschata] >XP_023548256.1 GDP-mann... [more]
XP_022981559.16.31e-25895.42GDP-mannose 4,6 dehydratase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0A0KY329.74e-271100.00GDP-mannose 4,6-dehydratase OS=Cucumis sativus OX=3659 GN=Csa_4G046810 PE=3 SV=1[more]
A0A5D3D1T23.96e-27099.73GDP-mannose 4,6-dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3BT163.96e-27099.73GDP-mannose 4,6-dehydratase OS=Cucumis melo OX=3656 GN=LOC103493184 PE=3 SV=1[more]
A0A6J1FR391.07e-25895.42GDP-mannose 4,6-dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111447684 PE=3 S... [more]
A0A6J1J2733.05e-25895.42GDP-mannose 4,6-dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111480638 PE=3 SV=... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 62..368
e-value: 2.6E-130
score: 436.0
NoneNo IPR availableGENE3D3.90.25.10coord: 252..378
e-value: 2.6E-130
score: 436.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 37..56
NoneNo IPR availablePANTHERPTHR43715:SF3GDP-MANNOSE 4,6 DEHYDRATASE 1-LIKEcoord: 47..399
NoneNo IPR availableCDDcd05260GDP_MD_SDR_ecoord: 60..384
e-value: 0.0
score: 540.648
IPR006368GDP-mannose 4,6-dehydrataseTIGRFAMTIGR01472TIGR01472coord: 60..385
e-value: 2.1E-170
score: 564.3
IPR006368GDP-mannose 4,6-dehydratasePANTHERPTHR43715GDP-MANNOSE 4,6-DEHYDRATASEcoord: 47..399
IPR006368GDP-mannose 4,6-dehydrataseHAMAPMF_00955GDP_Man_dehydratasecoord: 59..388
score: 56.050652
IPR016040NAD(P)-binding domainPFAMPF16363GDP_Man_Dehydcoord: 62..378
e-value: 1.6E-136
score: 455.1
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 59..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G12000.T1Cucsat.G12000.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042351 'de novo' GDP-L-fucose biosynthetic process
biological_process GO:0019673 GDP-mannose metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008446 GDP-mannose 4,6-dehydratase activity