Cucsat.G11788 (gene) Cucumber (B10) v3

Overview
NameCucsat.G11788
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionProtein kinase domain-containing protein
Locationctg1820: 5200017 .. 5204805 (+)
RNA-Seq ExpressionCucsat.G11788
SyntenyCucsat.G11788
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAACTAAACTTGGGATTAGACAAAAAAAAGCATAGCCTAATCCACCTTGTATGTAGGAGACCATTGGATTTCACAAGCCAAAAAGGGTCTCCCCCTCTAATTTCTCTTATGGGATTCTCACTTTGAAACTCATTTTCTTTCCTTTTCCTCTTCTCCAAAGGTAAATTCTTCTTTCTTCTTCTTCTTCTTAATCATTATTAATATTTAAATTAATAATCCCTTAATGCCTTTGTTCTTTTTAATTATTTCTTCAGGAAGTTGGTGAAATTTCCGGCAAAAATTATTTTCGGGTTAAATCTTGGCCGGAATTTCACTGCTTTGATAAGAGAAAGATGAGTTCAAGTGATGATGAGAGTGCTTCAGGGCCGGACTCTCAATCTTCACCTGCCGCTCCATCGCCGGATTCTTCGTCTTCGTCTTCACCTGATCCACCCTCATCGTCGCCGCCCTCACCGCCATCGCCATCTCCACCATCTCCCGATGCCAATTCCGATACCTCTCCTCCACCATCTCCAGAAAACGATGATTCCTCACCACCACCACCCACATCAGGTTCTATTAAATCATCATCATCTTCATCTTCTTTCTTCTCTCGATCGCTTTGAGTTTCTATTTTGTTCAATTGTACTTTTAATAAATATTTGCAATTTTAACTCTATATTTTCTCTTATCCATTTGTAAACTTTTATAGAAATTTGAGAACTAATTCTTTTTAGTTAAAATTTATATATGATGACAAAATTGTTCCATAGGTAGTTAATATATTTTTACTATTCAAAATATTTACAAAAATATATCAAAATATCATATTTTATCTAAATATACATTCTTGTTAGCGTCTATAAAATACATTAGTGGTAGTCTATCATACCTATATTGTGTGATAGTTACTATACTTTGTAAATAATTTTAAAAAAATTATCATTTAAAATATTTTTTCTATATTAACTATATTATCATACTTATTTTTCAAAACTTAAAATGGTTACCTTAGTTTGGGAAGTTTCTGGCACCAATTAAATCTTGTAAGAAAAATAATTGTAAATAGTAAAATTAGTTTGTGAATGTCATATGCAGGTGAAATATGAAATTTTATATTAGCTATATTTTATAAATATTTTGAAACATTAAAAAAATATACAAAGAAAAATTAGTTGATGAAATAGTATATATAATAAATTGTGAAGAAAAAAATATAGTAACACACTGCTTTAATTTTTTATTCTCCTACCACATAAAACATAAATTGAGGTACACTAAATATGGAGTTTTAAAATTCTGAGATGTTTAAGGCCAGACTTGGATTTTGGAGAAGCAATTCTTGGTTCTTCATTTTGGTTCTTTCTTATAAGATGTGAGTTATGAATTACAATCTAATTTTATTTATATATACATGTAATATATTATCATACGGTAGATAATTCTCCATCTCCAAAGTCCCCATCCGATGGTTCTTCCGATGATCACAAATCCCATCCAGCTCCAAAAGAGGGTGGTAGCAGTCCACTGACGTCACCCGCTGCTCATTCTCCTCCATCCCGATCTTCGTCAGCGGGGGACGAATTTTCTCCACCAGCTCTGCCGGAAGGAAAGGCATCACCAAGATCGCCAGCTAAACAACAAGGAACACCGTCCTCTGAGAAGGATTCATCGCCATCGGAAATCAACCCGGGGATTATAATTGGAGTGGCAGTGGGAGTGGGGGTTTTCATAATTGTTCTCATATCAGTAATTGCAGTTTGTTCAAAGAAGAAGAAGAGAAAGAGAGATCCTATGCCATATTATGGAGATCATGGTCATGGAGGACCTAAAGGTAAATTCCTTTTCTTGTTTTTTTTTTTTTTAAAAATCACTTTTGTCCTATTTAAAAAATCATTAAGGAAAACGTTTTTACTTTAGCTAAATTTTGTTTAATGTTCGATTTTACTCTTTAAAAATTTGAAAACCTTTTGAAAATTAACTCACTCTTTGTATGTACAAAGAAAATATGTCAACAACTAGACCAATGTAAATATGGTAACATTAAAAAGGTATAAGATAAAAGACAACTACTTACGTTTATATCATGACATTGATTAACATGCAGGTGGTGACTATTACAACAGTTCACTACAGCAACAACAAAACTGGCACAACGGCATGCACGGTCCCGGGACGGATCAATTTGGGAGGCCACCAGTATCGGGGTCTCCGGCCGGGACTGGGTGGCCAATGAGCGGCGAGAACATGACGACAGGTAGCTCCTATGCAGGGCCTCCTCTTCCTCCACCATCACCATCGATTGCTTTTGGGTTCAACAAGAGCACGTTCACGTACGACGAACTCGCTGCAGCAACAGGTGGGTTTGCTCATGGAAACCTACTCGGGCAGGGTGGGTTCGGTTATGTGCACAAGGGGGTACTGCCCAATGGGAAGGAAGTGGCAGTGAAAAGCTTGAAGGTGGGCAGTGGGCAGGGTGAGAGGGAGTTCATGGCTGAAGTTGAGATTATTAGTAGAGTCCATCATCGGCATTTGGTGTCTTTGGTTGGGTTTTGCATTGCTGGTGGACAAAGAATGTTGGTCTATGAGTTCGTTCCTAACAATACAATGGAGCATCATCTTCATGGTATTACTTTATCCCTTAAATTATGCAAATTCCATTCCTATTTTAAAGAAAATTTAGTTTAGATACGAAACCGTAACTATTTCTGTGCTTTTATGCAATTTTATCAAACGATTCCAACTGTTTTTAACTTTTAAAATTAATATAGGTGTTTGAATTTTTAACTTCCTTCAAGCTGTAATGAACAAATATAGAAAATAAATGTCTTTTTAAAGATTTTATTGACCTTTGAAAGTTAATTCTATCACTCAATTTTGGTTTCAGCCAAAGGTCTTCCAGTAATGGATTGGCCCGCTAGACTTCGCATTGCCATAGGTTCTGCCAAGGGACTTGCTTATCTTCACGAAGATTGTAAGAATTTTTTCTCTTTTATGGTACGCTAATTGTGTGGATGGGAGAATCAAAACCTCCACATCAAAAATGATATATCGTATCAATTACTCTTGAGTTAAATTCACTTTAGCTGTCAAAACCGACTTTATAACTTGTACATATAGAATTTCTCTTAATGGCTAGTTTAAAAATATATTGTAATTAAGTACTAATGGTTTATTGTCCAATTTCATAACACCCTTGCCTTTAATTTCATGTTTTTGAAATTTAGGTTTATTTTCAACACTCTTTCCTTCTCCTTCGGGAATTGTTTAATAAAGAGTAATATAATATATATTTCATACTTCATAGAATAATTGACAAAGAAATATGAAAACTTCTTCTTTTCAGGTCATCCAAAGATTATTCATCGAGATATCAAGTCAGCTAATATTCTGATCGATGCCAATTTTGAAGCTATGGTATGTTTAATAAAGAAACCCGACATGTTGAATAATAGTTTATCAATTGACATATAGTTTGTGTTACATTGATGAGAATTCAAGCCTAATTGTGTTCGATGGGGCATGATAATTTCAGGTGGCTGATTTTGGATTGGCTAAGTTGTCTACGGATAATCACACACACGTCTCAACTAGAGTCATGGGTACATTCGGGTAAGTAAAATTTAAACCACGCCTCTTATTTAGTAACACAAACATGTGTGTTGTTGAGTTATGCTTGCATGTCTAACTAAAAATGTTGTATAGGGATTAATGTTTTTGTTTATACTGGTACATATAATTAATTCAGGTATTTGGCACCAGAATACGCATCAAGCGGAAAGCTAACAGAAAAGTCTGACGTGTTCTCATTTGGTGTAATGTTGTTGGAACTCATTACAGGAAAACGTCCTGTAGATCCTACACATACGATGGAGGATAGCTTAGTTGATTGGGTACGTATACCTCCTACAAATAGTCTACTTTACAAATTTAACTCTTTAACTACAAAAAAGTTTAACAAGTTTAATTTATCAACTTTCAACACGTCTTCCATTTAAAAAGTGCATGATACATTTGTGATAAAACATAGTTTAAATTAAAAGTTTAAAAAGTCCAAATTAATGTACAACAGATTACAAGTTTAACCAAAATAATTGACATTATCGACGATTTATAATGTAGGCTCGACCACTAATGACTCGAGCATTAATGGAAGGGATATACGACGAGTTGGTGGATATACGTTTGGAGAGAGAGTTCAACACACAAGAAATGGCTCGAATGGTGGCTTGTGCTGCAGCCTCCATACGCCACTCGGCTAGAAAACGCCCAAAAATGAGCCAAGTGGTTCGAGCTTTAGAAGGAGATGTCTCACTGGACGACTTAAACGAAGGCACGAGACCAGGCCAAAGCTCCATCTTTAGCGCCACTAGCTCCGACTACGATTCTAGCGCCTACCATGCTGACATGTCGAAATTTAGGAAGATCGCTTTGTCCGACGACAGCAGTGAACTTGCAACTTCAAGCAACGATTCCCGAGAAATGAACCATCCCGGCCCCGGGACCCAAAGGCCGCTCTTTTAATCTTTTTTTTTTTGTCACTAATATATTTATCAAAATTGTTTAATTACATAGGAAAAGGGGGAAACAAAAAAGGATAAATATATGAGTTTGTTTTAATTTCTATGGTCGATTGAAAACTGTGATGTGGTGATCGTGGCAAATAACCAAGAAGGATATGGATCAAATGTGGTTTTATTAAAGTTTGTTTTTCTTTCTAAATTTTGTTTAGGTTTTGTTTTGATCGTCATAATATCTGTAGATATGTTGTGTCTTCATTTTTGGAGGATTGATTGATTGTTTGTTTCA

Coding sequence (CDS)

ATGAGTTCAAGTGATGATGAGAGTGCTTCAGGGCCGGACTCTCAATCTTCACCTGCCGCTCCATCGCCGGATTCTTCGTCTTCGTCTTCACCTGATCCACCCTCATCGTCGCCGCCCTCACCGCCATCGCCATCTCCACCATCTCCCGATGCCAATTCCGATACCTCTCCTCCACCATCTCCAGAAAACGATGATTCCTCACCACCACCACCCACATCAGATAATTCTCCATCTCCAAAGTCCCCATCCGATGGTTCTTCCGATGATCACAAATCCCATCCAGCTCCAAAAGAGGGTGGTAGCAGTCCACTGACGTCACCCGCTGCTCATTCTCCTCCATCCCGATCTTCGTCAGCGGGGGACGAATTTTCTCCACCAGCTCTGCCGGAAGGAAAGGCATCACCAAGATCGCCAGCTAAACAACAAGGAACACCGTCCTCTGAGAAGGATTCATCGCCATCGGAAATCAACCCGGGGATTATAATTGGAGTGGCAGTGGGAGTGGGGGTTTTCATAATTGTTCTCATATCAGTAATTGCAGTTTGTTCAAAGAAGAAGAAGAGAAAGAGAGATCCTATGCCATATTATGGAGATCATGGTCATGGAGGACCTAAAGGTGGTGACTATTACAACAGTTCACTACAGCAACAACAAAACTGGCACAACGGCATGCACGGTCCCGGGACGGATCAATTTGGGAGGCCACCAGTATCGGGGTCTCCGGCCGGGACTGGGTGGCCAATGAGCGGCGAGAACATGACGACAGGTAGCTCCTATGCAGGGCCTCCTCTTCCTCCACCATCACCATCGATTGCTTTTGGGTTCAACAAGAGCACGTTCACGTACGACGAACTCGCTGCAGCAACAGGTGGGTTTGCTCATGGAAACCTACTCGGGCAGGGTGGGTTCGGTTATGTGCACAAGGGGGTACTGCCCAATGGGAAGGAAGTGGCAGTGAAAAGCTTGAAGGTGGGCAGTGGGCAGGGTGAGAGGGAGTTCATGGCTGAAGTTGAGATTATTAGTAGAGTCCATCATCGGCATTTGGTGTCTTTGGTTGGGTTTTGCATTGCTGGTGGACAAAGAATGTTGGTCTATGAGTTCGTTCCTAACAATACAATGGAGCATCATCTTCATGCCAAAGGTCTTCCAGTAATGGATTGGCCCGCTAGACTTCGCATTGCCATAGGTTCTGCCAAGGGACTTGCTTATCTTCACGAAGATTGTCATCCAAAGATTATTCATCGAGATATCAAGTCAGCTAATATTCTGATCGATGCCAATTTTGAAGCTATGGTGGCTGATTTTGGATTGGCTAAGTTGTCTACGGATAATCACACACACGTCTCAACTAGAGTCATGGGTACATTCGGGTATTTGGCACCAGAATACGCATCAAGCGGAAAGCTAACAGAAAAGTCTGACGTGTTCTCATTTGGTGTAATGTTGTTGGAACTCATTACAGGAAAACGTCCTGTAGATCCTACACATACGATGGAGGATAGCTTAGTTGATTGGGCTCGACCACTAATGACTCGAGCATTAATGGAAGGGATATACGACGAGTTGGTGGATATACGTTTGGAGAGAGAGTTCAACACACAAGAAATGGCTCGAATGGTGGCTTGTGCTGCAGCCTCCATACGCCACTCGGCTAGAAAACGCCCAAAAATGAGCCAAGTGGTTCGAGCTTTAGAAGGAGATGTCTCACTGGACGACTTAAACGAAGGCACGAGACCAGGCCAAAGCTCCATCTTTAGCGCCACTAGCTCCGACTACGATTCTAGCGCCTACCATGCTGACATGTCGAAATTTAGGAAGATCGCTTTGTCCGACGACAGCAGTGAACTTGCAACTTCAAGCAACGATTCCCGAGAAATGAACCATCCCGGCCCCGGGACCCAAAGGCCGCTCTTTTAA

Protein sequence

MSSSDDESASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPPSPENDDSSPPPPTSDNSPSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAAHSPPSRSSSAGDEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVLISVIAVCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRPPVSGSPAGTGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHADMSKFRKIALSDDSSELATSSNDSREMNHPGPGTQRPLF
Homology
BLAST of Cucsat.G11788 vs. ExPASy Swiss-Prot
Match: Q9ZNQ8 (Proline-rich receptor-like protein kinase PERK4 OS=Arabidopsis thaliana OX=3702 GN=PERK4 PE=1 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 1.8e-172
Identity = 379/645 (58.76%), Postives = 454/645 (70.39%), Query Frame = 0

Query: 3   SSDDESASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPP--- 62
           +S  ESA   +S SSP+ PS  +S++SSP       PSPPSP+PP  D++S  SPPP   
Sbjct: 2   ASSPESAPPTNSTSSPSPPSNTNSTTSSP-----PAPSPPSPTPPQGDSSS--SPPPDST 61

Query: 63  SPENDDSSPPPPTSDNSPSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAAHSPPSRSS-- 122
           SP    +  PP +S+NSPSP S   G     + +     G  +P +  +  SPPSRS+  
Sbjct: 62  SPPAPQAPNPPNSSNNSPSPPSQGGGG---ERGNGGNNGGNDTPPSRGSPPSPPSRSNGD 121

Query: 123 SAGDEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVLIS 182
           + G   SPP    G  S   P+   G  S       S  N  II+GV VG G+ +IVL  
Sbjct: 122 NGGSRSSPPGDTGGSRSDNPPS--SGGSSGGGGGGRSNTNTAIIVGVLVGAGLLMIVL-- 181

Query: 183 VIAVCSKKKKRKRD---PMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGR 242
            I VC ++KK+++D   P P  G+       G +  N++ Q   NWH    G      G 
Sbjct: 182 -IIVCLRRKKKRKDSFYPEPMKGNQYQ--YYGNNNNNNASQNYPNWHLNSQGQNQQSTGG 241

Query: 243 PPVSG--SPAGTGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGF 302
               G   P     P SGE+ +  S  + P LPPPSP++A GFNKSTFTY ELAAATGGF
Sbjct: 242 WGGGGPSPPPPPRMPTSGEDSSMYSGPSRPVLPPPSPALALGFNKSTFTYQELAAATGGF 301

Query: 303 AHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLV 362
              NLLGQGGFGYVHKGVLP+GKEVAVKSLK GSGQGEREF AEV+IISRVHHR+LVSLV
Sbjct: 302 TDANLLGQGGFGYVHKGVLPSGKEVAVKSLKAGSGQGEREFQAEVDIISRVHHRYLVSLV 361

Query: 363 GFCIAGGQRMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKI 422
           G+CIA GQRMLVYEFVPN T+E+HLH K LPVM++  RLRIA+G+AKGLAYLHEDCHP+I
Sbjct: 362 GYCIADGQRMLVYEFVPNKTLEYHLHGKNLPVMEFSTRLRIALGAAKGLAYLHEDCHPRI 421

Query: 423 IHRDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEK 482
           IHRDIKSANIL+D NF+AMVADFGLAKL++DN+THVSTRVMGTFGYLAPEYASSGKLTEK
Sbjct: 422 IHRDIKSANILLDFNFDAMVADFGLAKLTSDNNTHVSTRVMGTFGYLAPEYASSGKLTEK 481

Query: 483 SDVFSFGVMLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFN 542
           SDVFS+GVMLLELITGKRPVD + TM+D+LVDWARPLM RAL +G ++EL D RLE  +N
Sbjct: 482 SDVFSYGVMLLELITGKRPVDNSITMDDTLVDWARPLMARALEDGNFNELADARLEGNYN 541

Query: 543 TQEMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSA--TSS 602
            QEMARMV CAAASIRHS RKRPKMSQ+VRALEG+VSLD LNEG +PG S+++ +   SS
Sbjct: 542 PQEMARMVTCAAASIRHSGRKRPKMSQIVRALEGEVSLDALNEGVKPGHSNVYGSLGASS 601

Query: 603 DYDSSAYHADMSKFRKIALSDDSSELA----TSSNDSREMNHPGP 632
           DY  ++Y+ADM KFR+IALS     ++    TSSNDSR+M    P
Sbjct: 602 DYSQTSYNADMKKFRQIALSSQEFPVSDCEGTSSNDSRDMGTKSP 629

BLAST of Cucsat.G11788 vs. ExPASy Swiss-Prot
Match: Q9XI96 (Proline-rich receptor-like protein kinase PERK7 OS=Arabidopsis thaliana OX=3702 GN=PERK7 PE=2 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 2.0e-163
Identity = 371/689 (53.85%), Postives = 438/689 (63.57%), Query Frame = 0

Query: 34  PSSSPPSPPSPSPPSPDANSD---TSPPPSPENDDSSPPPPTS----------DNSPSPK 93
           P +SPP+PP PSPPSP +++D   TSPPPS   + +SPPPP+S            SP P 
Sbjct: 7   PENSPPAPPPPSPPSPPSSNDQQTTSPPPSDNQETTSPPPPSSPDIAPPPQQQQESPPPP 66

Query: 94  SPSDGSSDDHKSHPAPKEGGSSPLTS--PAAHSPPSRSSSAGDEFS-------------- 153
            P + S     S P P    SS   S  P + SPP +S + G++ +              
Sbjct: 67  LPENSSDGSSSSSPPPPSDSSSQSQSPPPPSTSPPQQSDNNGNKGNNNENNKGNDGSSGD 126

Query: 154 ----------PPALPEGKASPRSPAKQQGTPSSEKDSSPSE---INPGIIIGVAVGVGVF 213
                     PP       S  S  +    P+S   S+ S    +N G +IG+    G+ 
Sbjct: 127 GGNKNMSHTPPPPSKTSDHSSHSQPRSLAPPTSNSGSNSSSNDGLNIGAVIGLVAAAGIL 186

Query: 214 IIVLISVIAVC--SKKKKRKRDPMPYYGDHGH-GGPKGGDYY---NSSLQQQQNWHNGMH 273
            IV+I +   C   KKKK K D MPYYG + +  G  GGD Y   N++ QQQQ+++   H
Sbjct: 187 FIVMILLCVCCFRKKKKKSKLDQMPYYGSNAYPAGKTGGDQYYNQNAATQQQQHYNQNDH 246

Query: 274 ---------GPGTDQFGRPPVSGSPAGTGWPM---------------SGENMTTGSS--Y 333
                      GT+    PP    P G   PM               SGE  +  SS  Y
Sbjct: 247 IVNLPPPPGSMGTNWVSSPP--PPPPGNWQPMPSPPAPVSGGANVIQSGEMSSNFSSGPY 306

Query: 334 AGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAV 393
           A P LPPP PS+A GFN STFTY+ELA+AT GF+   LLGQGGFGYVHKG+LPNGKE+AV
Sbjct: 307 A-PSLPPPHPSVALGFNNSTFTYEELASATQGFSKDRLLGQGGFGYVHKGILPNGKEIAV 366

Query: 394 KSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCI-AGGQRMLVYEFVPNNTMEHHLH 453
           KSLK GSGQGEREF AEVEIISRVHHRHLVSLVG+C  AGGQR+LVYEF+PN+T+E HLH
Sbjct: 367 KSLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCSNAGGQRLLVYEFLPNDTLEFHLH 426

Query: 454 AKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLA 513
            K   VMDWP RL+IA+GSAKGLAYLHEDCHPKIIHRDIK++NIL+D NFEA VADFGLA
Sbjct: 427 GKSGTVMDWPTRLKIALGSAKGLAYLHEDCHPKIIHRDIKASNILLDHNFEAKVADFGLA 486

Query: 514 KLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHTM 573
           KLS DN+THVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITG+ PVD +  M
Sbjct: 487 KLSQDNNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGRGPVDLSGDM 546

Query: 574 EDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMVACAAASIRHSARKRPKMS 633
           EDSLVDWARPL  R   +G Y ELVD  LE ++   EMARMVACAAA++RHS R+RPKMS
Sbjct: 547 EDSLVDWARPLCMRVAQDGEYGELVDPFLEHQYEPYEMARMVACAAAAVRHSGRRRPKMS 606

Query: 634 QVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHADMSKFRKIALSD----DSS 636
           Q+VR LEGD SLDDL++G +P QSS     SSDY+   Y A+M KFRK+ L       SS
Sbjct: 607 QIVRTLEGDASLDDLDDGVKPKQSSSGGEGSSDYEMGTYGAEMRKFRKVTLESRDYGASS 666

BLAST of Cucsat.G11788 vs. ExPASy Swiss-Prot
Match: Q9LS95 (Putative proline-rich receptor-like protein kinase PERK6 OS=Arabidopsis thaliana OX=3702 GN=PERK6 PE=2 SV=2)

HSP 1 Score: 561.2 bits (1445), Expect = 1.5e-158
Identity = 356/665 (53.53%), Postives = 446/665 (67.07%), Query Frame = 0

Query: 2   SSSDDESASGP-----DSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSP------------ 61
           SSSD++  S P      S S PA P PD SS+ SP PPSS   SPPSP            
Sbjct: 20  SSSDNQQQSSPPPSDSSSPSPPAPPPPDDSSNGSPQPPSSDSQSPPSPQGNNNNDGNNGN 79

Query: 62  ------SPPSPDANSDTSPPPSPENDDSSPPPPTSDNSPSPKSPSDGSSDDHKSHPAPKE 121
                 +  + + N+D +   + +N+++      ++N+ +  + ++ + +++ ++     
Sbjct: 80  NNNDNNNNNNGNNNNDNNNGNNKDNNNNGNNNNGNNNNGNDNNGNNNNGNNNDNNNQNNG 139

Query: 122 GGSSPLTSPAAHSPPSRSSSAGDEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINP 181
           GGS+  + P    PPSR+S    + + P+ P   A PRS      +      S  +E N 
Sbjct: 140 GGSNNRSPP----PPSRNS----DRNSPSPPRALAPPRS------SGGGSNSSGNNEPNT 199

Query: 182 GIIIGVAVGVGVFIIVLISVIAVCSKKKKRKRDPMPYYGDHGHGGPKGGDY----YNS-- 241
             I+G+  G G+  +V+I +  VC  +KK+K+  MPYY  +G+   KG  Y    YN+  
Sbjct: 200 AAIVGIVAGAGLLFLVMI-LFCVCCCRKKKKKHQMPYYAGNGYATGKGDQYQQQQYNNQS 259

Query: 242 ----SLQQQQNWHNGMHG---------PGTDQFGRPPVSGSPAGTGWPMSGENMTTGSSY 301
               +L QQ    NG +          PG+ Q   PP    P      M+G +    S+Y
Sbjct: 260 DHVMNLSQQYPGSNGNNNWMNSPPPPPPGSWQPSPPP---PPPPVSGGMNGNSSDFSSNY 319

Query: 302 A---GPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKE 361
           +   GP +PPP PS+A GFNKSTFTYDELAAAT GF+   LLGQGGFGYVHKG+LPNGKE
Sbjct: 320 SGPHGPSVPPPHPSVALGFNKSTFTYDELAAATQGFSQSRLLGQGGFGYVHKGILPNGKE 379

Query: 362 VAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTMEHH 421
           +AVKSLK GSGQGEREF AEV+IISRVHHR LVSLVG+CIAGGQRMLVYEF+PN+T+E H
Sbjct: 380 IAVKSLKAGSGQGEREFQAEVDIISRVHHRFLVSLVGYCIAGGQRMLVYEFLPNDTLEFH 439

Query: 422 LHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFG 481
           LH K   V+DWP RL+IA+GSAKGLAYLHEDCHP+IIHRDIK++NIL+D +FEA VADFG
Sbjct: 440 LHGKSGKVLDWPTRLKIALGSAKGLAYLHEDCHPRIIHRDIKASNILLDESFEAKVADFG 499

Query: 482 LAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTH 541
           LAKLS DN THVSTR+MGTFGYLAPEYASSGKLT++SDVFSFGVMLLEL+TG+RPVD T 
Sbjct: 500 LAKLSQDNVTHVSTRIMGTFGYLAPEYASSGKLTDRSDVFSFGVMLLELVTGRRPVDLTG 559

Query: 542 TMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMVACAAASIRHSARKRPK 601
            MEDSLVDWARP+   A  +G Y ELVD RLE ++   EMA+MVACAAA++RHSAR+RPK
Sbjct: 560 EMEDSLVDWARPICLNAAQDGDYSELVDPRLENQYEPHEMAQMVACAAAAVRHSARRRPK 619

Query: 602 MSQVVRALEGDVSLDDLNEGTRPGQSSIFS-ATSSDYDSSAYHADMSKFRKIALSDDSSE 621
           MSQ+VRALEGD +LDDL+EG + GQSS     +SSDYDSS Y ADM KFRK+AL  DS E
Sbjct: 620 MSQIVRALEGDATLDDLSEGGKAGQSSFLGRGSSSDYDSSTYSADMKKFRKVAL--DSHE 664

BLAST of Cucsat.G11788 vs. ExPASy Swiss-Prot
Match: Q8GX23 (Proline-rich receptor-like protein kinase PERK5 OS=Arabidopsis thaliana OX=3702 GN=PERK5 PE=2 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.8e-156
Identity = 363/680 (53.38%), Postives = 455/680 (66.91%), Query Frame = 0

Query: 1   MSSSDDESASGPD-SQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPP 60
           M+ S  +S+  P+ S  +P +     S+ SSP  P SSPP P S S P PD ++  SPPP
Sbjct: 1   MADSPVDSSPAPETSNGTPPSNGTSPSNESSPPTPPSSPP-PSSISAPPPDISASFSPPP 60

Query: 61  SPENDDSSPPPPTSDNSPSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAAHSPPSRSSSA 120
           +P   ++SPP   S + P   +PS   + ++ S PAP+  GS+P+T PA    PS  S  
Sbjct: 61  APPTQETSPPTSPSSSPPVVANPSP-QTPENPSPPAPE--GSTPVTPPAPPQTPSNQSPE 120

Query: 121 ------------------------GDEFSPPA---LPEGKASPRSPAKQQGTPSSEKDSS 180
                                   G   SPP+      G     SP +    P +  DS 
Sbjct: 121 RPTPPSPGANDDRNRTNGGNNNRDGSTPSPPSSGNRTSGDGGSPSPPRSISPPQNSGDSD 180

Query: 181 PS-----EINPGIIIGVAVGVGVFIIVLISVIAVCSKKKKRKR---DPMPYYGDHGHGG- 240
            S     + N G+IIGV VG G+ +++ + +   C++KKK+K    + M YY ++ +GG 
Sbjct: 181 SSSGNHPQANIGLIIGVLVGAGLLLLLAVCICICCNRKKKKKSPQVNHMHYYNNNPYGGA 240

Query: 241 PKG-GDYYNSSLQQQQNWHNGMHGPGTDQFG-RPPVSGSPAGTGWPMSGENMTTGSSYAG 300
           P G G YY  +    Q+    M G G   +G + PVSG       P S  +  TG +   
Sbjct: 241 PSGNGGYYKGT---PQDHVVNMAGQGGGNWGPQQPVSG-------PHSDASNLTGRTAI- 300

Query: 301 PPLPPPSPSIA-FGFNKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVK 360
                PSP  A  G N+STFTYDEL+ AT GFA  NLLGQGGFGYVHKGVLP+GKEVAVK
Sbjct: 301 -----PSPQAATLGHNQSTFTYDELSIATEGFAQSNLLGQGGFGYVHKGVLPSGKEVAVK 360

Query: 361 SLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTMEHHLHAK 420
           SLK+GSGQGEREF AEV+IISRVHHRHLVSLVG+CI+GGQR+LVYEF+PNNT+E HLH K
Sbjct: 361 SLKLGSGQGEREFQAEVDIISRVHHRHLVSLVGYCISGGQRLLVYEFIPNNTLEFHLHGK 420

Query: 421 GLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKL 480
           G PV+DWP R++IA+GSA+GLAYLHEDCHP+IIHRDIK+ANIL+D +FE  VADFGLAKL
Sbjct: 421 GRPVLDWPTRVKIALGSARGLAYLHEDCHPRIIHRDIKAANILLDFSFETKVADFGLAKL 480

Query: 481 STDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHTMED 540
           S DN+THVSTRVMGTFGYLAPEYASSGKL++KSDVFSFGVMLLELITG+ P+D T  MED
Sbjct: 481 SQDNYTHVSTRVMGTFGYLAPEYASSGKLSDKSDVFSFGVMLLELITGRPPLDLTGEMED 540

Query: 541 SLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMVACAAASIRHSARKRPKMSQV 600
           SLVDWARPL  +A  +G Y++L D RLE  ++ QEM +M +CAAA+IRHSAR+RPKMSQ+
Sbjct: 541 SLVDWARPLCLKAAQDGDYNQLADPRLELNYSHQEMVQMASCAAAAIRHSARRRPKMSQI 600

Query: 601 VRALEGDVSLDDLNEGTRPGQSSIFS--ATSSDYDSSAYHADMSKFRKIALSD---DSSE 628
           VRALEGD+S+DDL+EGTRPGQS+  S  + SS+YD+S+Y ADM KF+K+AL +    SSE
Sbjct: 601 VRALEGDMSMDDLSEGTRPGQSTYLSPGSVSSEYDASSYTADMKKFKKLALENKEYQSSE 660

BLAST of Cucsat.G11788 vs. ExPASy Swiss-Prot
Match: Q9LV48 (Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 GN=PERK1 PE=1 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 4.4e-155
Identity = 336/619 (54.28%), Postives = 437/619 (70.60%), Query Frame = 0

Query: 8   SASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPPSPENDDSS 67
           +A  P +  SP+ PSP ++S+++  PP++S P PP+ +P        +SPPPSP  + +S
Sbjct: 3   TAPSPGTTPSPSPPSPPTNSTTTTPPPAASSP-PPTTTP--------SSPPPSPSTNSTS 62

Query: 68  PPPPTSDNSPSPKSPSDGSSDDHKSHPAPKEGGSSPLT--SPAAHSPPSRSSSAGDEFSP 127
           PPP    +SP P S    S     + P P+   S+P+T   P+  +P +  S       P
Sbjct: 63  PPP----SSPLPPSLPPPSPPGSLTPPLPQPSPSAPITPSPPSPTTPSNPRSPPSPNQGP 122

Query: 128 PALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVLISVIAVCSKK 187
           P  P G ++PR+P+  + +P S+   S   ++ G+++G+A+G GV I+V++++I +  KK
Sbjct: 123 PNTPSG-STPRTPSNTKPSPPSD---SSDGLSTGVVVGIAIG-GVAILVILTLICLLCKK 182

Query: 188 KKRKR--DPMPYY-GDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQF-------GRP 247
           K+R+R  D   YY       GPK G  Y     QQQ W        +D          +P
Sbjct: 183 KRRRRHDDEAAYYVPPPPPSGPKAGGPYGG---QQQYWQQQNASRPSDNHVVTSLPPPKP 242

Query: 248 P-VSGSPAGTGWPMSGENMTTGSSYAG-PPLPPPSPSIAFGFNKSTFTYDELAAATGGFA 307
           P     P     P +  + + GS Y+  P LPPPSP +  GF+KSTFTY+EL+ AT GF+
Sbjct: 243 PSPPRKPPPPPPPPAFMSSSGGSDYSDLPVLPPPSPGLVLGFSKSTFTYEELSRATNGFS 302

Query: 308 HGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVG 367
             NLLGQGGFGYVHKG+LP+GKEVAVK LK GSGQGEREF AEVEIISRVHHRHLVSL+G
Sbjct: 303 EANLLGQGGFGYVHKGILPSGKEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSLIG 362

Query: 368 FCIAGGQRMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKII 427
           +C+AG QR+LVYEFVPNN +E HLH KG P M+W  RL+IA+GSAKGL+YLHEDC+PKII
Sbjct: 363 YCMAGVQRLLVYEFVPNNNLEFHLHGKGRPTMEWSTRLKIALGSAKGLSYLHEDCNPKII 422

Query: 428 HRDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKS 487
           HRDIK++NILID  FEA VADFGLAK+++D +THVSTRVMGTFGYLAPEYA+SGKLTEKS
Sbjct: 423 HRDIKASNILIDFKFEAKVADFGLAKIASDTNTHVSTRVMGTFGYLAPEYAASGKLTEKS 482

Query: 488 DVFSFGVMLLELITGKRPVDPTHT-MEDSLVDWARPLMTRALMEGIYDELVDIRLEREFN 547
           DVFSFGV+LLELITG+RPVD  +  ++DSLVDWARPL+ RA  EG ++ L D ++  E++
Sbjct: 483 DVFSFGVVLLELITGRRPVDANNVYVDDSLVDWARPLLNRASEEGDFEGLADSKMGNEYD 542

Query: 548 TQEMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSA--TSS 607
            +EMARMVACAAA +RHSAR+RP+MSQ+VRALEG+VSL DLNEG RPG S+++S+   S+
Sbjct: 543 REEMARMVACAAACVRHSARRRPRMSQIVRALEGNVSLSDLNEGMRPGHSNVYSSYGGST 600

Query: 608 DYDSSAYHADMSKFRKIAL 610
           DYD+S Y+ DM KFRK+AL
Sbjct: 603 DYDTSQYNDDMIKFRKMAL 600

BLAST of Cucsat.G11788 vs. NCBI nr
Match: XP_004149167.1 (proline-rich receptor-like protein kinase PERK4 [Cucumis sativus] >KGN53256.1 hypothetical protein Csa_014487 [Cucumis sativus])

HSP 1 Score: 1213 bits (3138), Expect = 0.0
Identity = 638/638 (100.00%), Postives = 638/638 (100.00%), Query Frame = 0

Query: 1   MSSSDDESASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPPS 60
           MSSSDDESASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPPS
Sbjct: 1   MSSSDDESASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPPS 60

Query: 61  PENDDSSPPPPTSDNSPSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAAHSPPSRSSSAG 120
           PENDDSSPPPPTSDNSPSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAAHSPPSRSSSAG
Sbjct: 61  PENDDSSPPPPTSDNSPSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAAHSPPSRSSSAG 120

Query: 121 DEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVLISVIA 180
           DEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVLISVIA
Sbjct: 121 DEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVLISVIA 180

Query: 181 VCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRPPVSGS 240
           VCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRPPVSGS
Sbjct: 181 VCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRPPVSGS 240

Query: 241 PAGTGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQ 300
           PAGTGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQ
Sbjct: 241 PAGTGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQ 300

Query: 301 GGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQ 360
           GGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQ
Sbjct: 301 GGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQ 360

Query: 361 RMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSA 420
           RMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSA
Sbjct: 361 RMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSA 420

Query: 421 NILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGV 480
           NILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGV
Sbjct: 421 NILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGV 480

Query: 481 MLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMV 540
           MLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMV
Sbjct: 481 MLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMV 540

Query: 541 ACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHAD 600
           ACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHAD
Sbjct: 541 ACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHAD 600

Query: 601 MSKFRKIALSDDSSELATSSNDSREMNHPGPGTQRPLF 638
           MSKFRKIALSDDSSELATSSNDSREMNHPGPGTQRPLF
Sbjct: 601 MSKFRKIALSDDSSELATSSNDSREMNHPGPGTQRPLF 638

BLAST of Cucsat.G11788 vs. NCBI nr
Match: XP_008453734.1 (PREDICTED: proline-rich receptor-like protein kinase PERK4 [Cucumis melo])

HSP 1 Score: 1074 bits (2777), Expect = 0.0
Identity = 587/645 (91.01%), Postives = 600/645 (93.02%), Query Frame = 0

Query: 1   MSSSDDESASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPPS 60
           MSSSD ES+ GPDSQSSPAAPSPDSSS SSPDPPS    SPP PSPPSPDANSD SPPPS
Sbjct: 1   MSSSDGESSPGPDSQSSPAAPSPDSSSPSSPDPPSQK--SPPLPSPPSPDANSDFSPPPS 60

Query: 61  PEND----DSSPPPPTSDNS-PSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAAHSPPSR 120
           PEND    +SSPPPP SDNS PSPKSPSDGSS+D KS PAPKEG  SP TSP AHSPPSR
Sbjct: 61  PENDGSSNNSSPPPPPSDNSSPSPKSPSDGSSNDQKSPPAPKEG--SPRTSPVAHSPPSR 120

Query: 121 SSSAGDEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVL 180
           SSS G EFSPPALPEGKASPRSPA+Q      EKDSSPSEINPGIIIGVAVGVGVFIIVL
Sbjct: 121 SSSPGHEFSPPALPEGKASPRSPARQHA----EKDSSPSEINPGIIIGVAVGVGVFIIVL 180

Query: 181 ISVIAVCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRP 240
           IS+IAVCSKKKKRKR+PMPYYGDHGHGGPK GDYYNS+ QQQQNWHNGMHGPGTDQFGRP
Sbjct: 181 ISLIAVCSKKKKRKREPMPYYGDHGHGGPKSGDYYNST-QQQQNWHNGMHGPGTDQFGRP 240

Query: 241 PVSGSPAGTG-WPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAH 300
           PVSGS  G G WPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAH
Sbjct: 241 PVSGSSPGPGGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAH 300

Query: 301 GNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGF 360
            NLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGF
Sbjct: 301 ANLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGF 360

Query: 361 CIAGGQRMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIH 420
           CIAGGQRMLVYEFVPNNT+EHHLH KGLPVMDW  RLRIAIGSAKGLAYLHEDCHPKIIH
Sbjct: 361 CIAGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWSTRLRIAIGSAKGLAYLHEDCHPKIIH 420

Query: 421 RDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSD 480
           RDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSD
Sbjct: 421 RDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSD 480

Query: 481 VFSFGVMLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQ 540
           VFSFGVMLLELITGKRPVDPTHTM+DSLVDWARPLMTRALM+G YDELVDIRLE++FNTQ
Sbjct: 481 VFSFGVMLLELITGKRPVDPTHTMDDSLVDWARPLMTRALMDGTYDELVDIRLEKDFNTQ 540

Query: 541 EMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDS 600
           EMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEG RPGQSSIFSA SSDYDS
Sbjct: 541 EMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGIRPGQSSIFSAASSDYDS 600

Query: 601 SAYHADMSKFRKIALSDDSSELATSSNDSREMNHPGPGT-QRPLF 638
           +AY ADMSKFRKIALSDDSSE ATSSNDSREMNHPGPGT QRPLF
Sbjct: 601 NAYQADMSKFRKIALSDDSSEHATSSNDSREMNHPGPGTTQRPLF 636

BLAST of Cucsat.G11788 vs. NCBI nr
Match: XP_038877288.1 (proline-rich receptor-like protein kinase PERK4 [Benincasa hispida])

HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 546/649 (84.13%), Postives = 568/649 (87.52%), Query Frame = 0

Query: 1   MSSSDDESASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPPS 60
           M S+ DESA GPDS S PA P  DSSSSSSPDPPSSSPPSPPSP     D +S+ SPPP 
Sbjct: 1   MDSNYDESAPGPDSPSLPADPPLDSSSSSSPDPPSSSPPSPPSP-----DPDSNFSPPPP 60

Query: 61  PEND----DSSPPPPTSDNSPSPKSPSDG---SSDDHKSHPAPKEGG--SSPLTSPAAHS 120
           PEND    DSSPPP    +SPSPKS  D     SD  KS  APKEG   S+PL S   HS
Sbjct: 61  PENDGSSNDSSPPPK---DSPSPKSSKDKLDKPSDSKKSPSAPKEGSPRSAPLNS---HS 120

Query: 121 PPSRSSSAGDEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVF 180
           PPSRSSS+ D    P   EGKASP+S A+QQ   SSE DSSPSEINPG+IIGVAVGVGV 
Sbjct: 121 PPSRSSSSADALPAPGQTEGKASPKSSARQQ---SSEADSSPSEINPGVIIGVAVGVGVL 180

Query: 181 IIVLISVIAVCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHN-GMHGPGTD 240
           IIVLISV+A+C+KKKKRK DP+ YYGDHG G P+GGDYYNSS  QQQNWHN GM GPGTD
Sbjct: 181 IIVLISVVALCTKKKKRKGDPVQYYGDHGRG-PRGGDYYNSS--QQQNWHNNGMQGPGTD 240

Query: 241 QFGRPPVSGSPAGTGWPMSG-ENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAAT 300
           QFGR P S S AG+GWPMSG +NMTTGSSY GPPLPPPSPSIAFGFNKSTFTYDELAAAT
Sbjct: 241 QFGRQPPSASLAGSGWPMSGGDNMTTGSSYTGPPLPPPSPSIAFGFNKSTFTYDELAAAT 300

Query: 301 GGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLV 360
           GGFAH NLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLV
Sbjct: 301 GGFAHANLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLV 360

Query: 361 SLVGFCIAGGQRMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCH 420
           SLVG+CIA GQRMLVYEFVPNNT+EHHLH KGLPVMDWP RLRIAIGSAKGLAYLHEDCH
Sbjct: 361 SLVGYCIASGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPTRLRIAIGSAKGLAYLHEDCH 420

Query: 421 PKIIHRDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKL 480
           PKIIHRDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKL
Sbjct: 421 PKIIHRDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKL 480

Query: 481 TEKSDVFSFGVMLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLER 540
           TEKSDVFSFGVMLLELI GKRPVDPT  MEDSLVDWARPLM+RALMEGIY+ELVDIRLE 
Sbjct: 481 TEKSDVFSFGVMLLELIAGKRPVDPTQAMEDSLVDWARPLMSRALMEGIYEELVDIRLEN 540

Query: 541 EFNTQEMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATS 600
           EFNTQEMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEG RPGQSSI+SATS
Sbjct: 541 EFNTQEMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGIRPGQSSIYSATS 600

Query: 601 SDYDSSAYHADMSKFRKIALSDDSSELATSSNDSREMNHPGPGTQRPLF 638
           SDYDS+AYHADMSKFRKIALSDDSSELA SSNDSREMNHP PGTQRPLF
Sbjct: 601 SDYDSNAYHADMSKFRKIALSDDSSELAASSNDSREMNHPSPGTQRPLF 632

BLAST of Cucsat.G11788 vs. NCBI nr
Match: XP_022136397.1 (proline-rich receptor-like protein kinase PERK4 [Momordica charantia] >XP_022136398.1 proline-rich receptor-like protein kinase PERK4 [Momordica charantia])

HSP 1 Score: 856 bits (2211), Expect = 2.11e-305
Identity = 492/668 (73.65%), Postives = 523/668 (78.29%), Query Frame = 0

Query: 43  SPSPPSPDANSDTSPPPSPENDDSSP------PPPTSDN-SPSPKSPSDGSSDD------ 102
           SPSPPSPDANSD SPPP P NDDSS       P P+SD+ S  PKS SDG S D      
Sbjct: 37  SPSPPSPDANSDYSPPPPPTNDDSSSSSSSSSPAPSSDSGSQPPKSSSDGGSSDGGSSNG 96

Query: 103 --------------------------------HKSHPAPKEGGS---------------- 162
                                           + S+ +  +GGS                
Sbjct: 97  GSSDRGSSNNDSSNRGSSNGGSSDGGSNRSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSK 156

Query: 163 --SPLTSPAA--HSPPSRSSSAGDEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPS-EI 222
             S  +SP     S PSRSS      SPP   EGK+S RS   + GT      S PS ++
Sbjct: 157 DGSSRSSPFVPDQSSPSRSSPQSGALSPPI--EGKSSSRSSGDKHGT------SEPSLDV 216

Query: 223 NPGIIIGVAVGVGVFIIVLISVIAVCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQ 282
           NPGIIIGVAVGVGVFII+LI+++AVCSKKKKRK DPM YY DHGHG PKGGDYYNSS QQ
Sbjct: 217 NPGIIIGVAVGVGVFIILLIAIVAVCSKKKKRKGDPMQYY-DHGHG-PKGGDYYNSS-QQ 276

Query: 283 QQNWHNGMHGPGTDQFGRPPVSGSPAGTGWP----MSGENMTTGSSYAGPPLPPPSPSIA 342
             NW NGMHGPGTDQFGRPP SGS AG+GWP    MSG+   +G+   GPPLPPPSPSIA
Sbjct: 277 PTNWQNGMHGPGTDQFGRPPPSGS-AGSGWPSPPPMSGDMTMSGNYTGGPPLPPPSPSIA 336

Query: 343 FGFNKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGERE 402
           F F+KSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGERE
Sbjct: 337 FAFSKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGERE 396

Query: 403 FMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTMEHHLHAKGLPVMDWPARLR 462
           FMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNT+EHHLH KGLPVMDWPARLR
Sbjct: 397 FMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLR 456

Query: 463 IAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRV 522
           I IGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLS+DNHTHVSTRV
Sbjct: 457 IGIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSSDNHTHVSTRV 516

Query: 523 MGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHTMEDSLVDWARPLMTR 582
           MGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTH MEDSLVDWARPLM R
Sbjct: 517 MGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNR 576

Query: 583 ALMEGIYDELVDIRLEREFNTQEMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDD 638
           AL EGIYD+LVD+RLE EFNTQEMARMVACAAA+IRHSARKRPKMSQ+VRALEGDVSLDD
Sbjct: 577 ALGEGIYDDLVDMRLENEFNTQEMARMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDD 636

BLAST of Cucsat.G11788 vs. NCBI nr
Match: KAG6600609.1 (Proline-rich receptor-like protein kinase PERK4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 793 bits (2047), Expect = 1.23e-281
Identity = 442/598 (73.91%), Postives = 482/598 (80.60%), Query Frame = 0

Query: 44  PSPPSPDANSDTSPPPSPENDDSSPPPPTSDNSPSPKSPSDGSS-DDHKSHPAPKEGGSS 103
           PSP  PD N D+S P    N   SPPP  SD SP+PKS  D       KS  AP    S+
Sbjct: 48  PSPSFPDFNPDSSTPSDDNNSGISPPP--SDESPAPKSSKDSKDRKSGKSSKAPSPRSST 107

Query: 104 PLTSPAAHSPPSRSSSAG-DEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGII 163
           P+   A+ SP  RSSS+  D  +     EGK+S +S  K     S+  DS  S+++PG+I
Sbjct: 108 PI---ASKSPSPRSSSSSKDALTSGDRTEGKSSHKSSHKS----SNNDDSGSSDVSPGVI 167

Query: 164 IGVAVGVGVFIIVLISVIAVCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQQQNWH 223
           IGVAVGVG+FII+LIS+IA+CSKKKKRK DPM YY      GPKGG+YYN S  Q+ NWH
Sbjct: 168 IGVAVGVGLFIILLISLIALCSKKKKRKEDPMSYYSGDNANGPKGGNYYNGS--QKPNWH 227

Query: 224 NGMHGPGTDQFGRPPVSGSPAGTGWP-MSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTF 283
           N   G            G P   GWP MS ENMTT  S+ GPPLPPPSPS+AFGF+KSTF
Sbjct: 228 NNNAG------------GGPGVNGWPPMSSENMTTSGSFTGPPLPPPSPSLAFGFSKSTF 287

Query: 284 TYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEII 343
           TYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEII
Sbjct: 288 TYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEII 347

Query: 344 SRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKG 403
           SRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNT+EHHLH K LPVMDWP RLRIAIGSAKG
Sbjct: 348 SRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTLEHHLHGKDLPVMDWPTRLRIAIGSAKG 407

Query: 404 LAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLA 463
           LAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLS+D +THVSTRVMGTFGYLA
Sbjct: 408 LAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSSDTNTHVSTRVMGTFGYLA 467

Query: 464 PEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYD 523
           PEYASSGKLTEKSDVFSFGVMLLELITGKRPVDP H MEDSLVDWAR +++  + E +Y+
Sbjct: 468 PEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPHHVMEDSLVDWARAIISGEMGEDVYE 527

Query: 524 ELVDIRLEREFNTQEMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPG 583
            LVD RL +EFNTQEM RMV+CAAASIRHSARKRPKMSQVVRALEGD SLDDLN+G RPG
Sbjct: 528 VLVDKRLGKEFNTQEMGRMVSCAAASIRHSARKRPKMSQVVRALEGDYSLDDLNDGIRPG 587

Query: 584 QSSIFSATSSDYDSSAYHADMSKFRKIALSDDSSELATSSNDSREMNHPGPGTQRPLF 638
           QSSI+S   SDYDS+AY ADMS+FRK+ALSDDSSELATSSNDSRE+NHPG  TQRPLF
Sbjct: 588 QSSIYSVAGSDYDSNAYQADMSRFRKLALSDDSSELATSSNDSREINHPG--TQRPLF 620

BLAST of Cucsat.G11788 vs. ExPASy TrEMBL
Match: A0A0A0KZE1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038730 PE=4 SV=1)

HSP 1 Score: 1213 bits (3138), Expect = 0.0
Identity = 638/638 (100.00%), Postives = 638/638 (100.00%), Query Frame = 0

Query: 1   MSSSDDESASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPPS 60
           MSSSDDESASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPPS
Sbjct: 1   MSSSDDESASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPPS 60

Query: 61  PENDDSSPPPPTSDNSPSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAAHSPPSRSSSAG 120
           PENDDSSPPPPTSDNSPSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAAHSPPSRSSSAG
Sbjct: 61  PENDDSSPPPPTSDNSPSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAAHSPPSRSSSAG 120

Query: 121 DEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVLISVIA 180
           DEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVLISVIA
Sbjct: 121 DEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVLISVIA 180

Query: 181 VCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRPPVSGS 240
           VCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRPPVSGS
Sbjct: 181 VCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRPPVSGS 240

Query: 241 PAGTGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQ 300
           PAGTGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQ
Sbjct: 241 PAGTGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQ 300

Query: 301 GGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQ 360
           GGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQ
Sbjct: 301 GGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQ 360

Query: 361 RMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSA 420
           RMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSA
Sbjct: 361 RMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSA 420

Query: 421 NILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGV 480
           NILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGV
Sbjct: 421 NILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGV 480

Query: 481 MLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMV 540
           MLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMV
Sbjct: 481 MLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMV 540

Query: 541 ACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHAD 600
           ACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHAD
Sbjct: 541 ACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHAD 600

Query: 601 MSKFRKIALSDDSSELATSSNDSREMNHPGPGTQRPLF 638
           MSKFRKIALSDDSSELATSSNDSREMNHPGPGTQRPLF
Sbjct: 601 MSKFRKIALSDDSSELATSSNDSREMNHPGPGTQRPLF 638

BLAST of Cucsat.G11788 vs. ExPASy TrEMBL
Match: A0A1S3BX25 (proline-rich receptor-like protein kinase PERK4 OS=Cucumis melo OX=3656 GN=LOC103494378 PE=4 SV=1)

HSP 1 Score: 1074 bits (2777), Expect = 0.0
Identity = 587/645 (91.01%), Postives = 600/645 (93.02%), Query Frame = 0

Query: 1   MSSSDDESASGPDSQSSPAAPSPDSSSSSSPDPPSSSPPSPPSPSPPSPDANSDTSPPPS 60
           MSSSD ES+ GPDSQSSPAAPSPDSSS SSPDPPS    SPP PSPPSPDANSD SPPPS
Sbjct: 1   MSSSDGESSPGPDSQSSPAAPSPDSSSPSSPDPPSQK--SPPLPSPPSPDANSDFSPPPS 60

Query: 61  PEND----DSSPPPPTSDNS-PSPKSPSDGSSDDHKSHPAPKEGGSSPLTSPAAHSPPSR 120
           PEND    +SSPPPP SDNS PSPKSPSDGSS+D KS PAPKEG  SP TSP AHSPPSR
Sbjct: 61  PENDGSSNNSSPPPPPSDNSSPSPKSPSDGSSNDQKSPPAPKEG--SPRTSPVAHSPPSR 120

Query: 121 SSSAGDEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVL 180
           SSS G EFSPPALPEGKASPRSPA+Q      EKDSSPSEINPGIIIGVAVGVGVFIIVL
Sbjct: 121 SSSPGHEFSPPALPEGKASPRSPARQHA----EKDSSPSEINPGIIIGVAVGVGVFIIVL 180

Query: 181 ISVIAVCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRP 240
           IS+IAVCSKKKKRKR+PMPYYGDHGHGGPK GDYYNS+ QQQQNWHNGMHGPGTDQFGRP
Sbjct: 181 ISLIAVCSKKKKRKREPMPYYGDHGHGGPKSGDYYNST-QQQQNWHNGMHGPGTDQFGRP 240

Query: 241 PVSGSPAGTG-WPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAH 300
           PVSGS  G G WPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAH
Sbjct: 241 PVSGSSPGPGGWPMSGENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAH 300

Query: 301 GNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGF 360
            NLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGF
Sbjct: 301 ANLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGF 360

Query: 361 CIAGGQRMLVYEFVPNNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIH 420
           CIAGGQRMLVYEFVPNNT+EHHLH KGLPVMDW  RLRIAIGSAKGLAYLHEDCHPKIIH
Sbjct: 361 CIAGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWSTRLRIAIGSAKGLAYLHEDCHPKIIH 420

Query: 421 RDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSD 480
           RDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSD
Sbjct: 421 RDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSD 480

Query: 481 VFSFGVMLLELITGKRPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQ 540
           VFSFGVMLLELITGKRPVDPTHTM+DSLVDWARPLMTRALM+G YDELVDIRLE++FNTQ
Sbjct: 481 VFSFGVMLLELITGKRPVDPTHTMDDSLVDWARPLMTRALMDGTYDELVDIRLEKDFNTQ 540

Query: 541 EMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDS 600
           EMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEG RPGQSSIFSA SSDYDS
Sbjct: 541 EMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDDLNEGIRPGQSSIFSAASSDYDS 600

Query: 601 SAYHADMSKFRKIALSDDSSELATSSNDSREMNHPGPGT-QRPLF 638
           +AY ADMSKFRKIALSDDSSE ATSSNDSREMNHPGPGT QRPLF
Sbjct: 601 NAYQADMSKFRKIALSDDSSEHATSSNDSREMNHPGPGTTQRPLF 636

BLAST of Cucsat.G11788 vs. ExPASy TrEMBL
Match: A0A6J1C7H0 (proline-rich receptor-like protein kinase PERK4 OS=Momordica charantia OX=3673 GN=LOC111008118 PE=4 SV=1)

HSP 1 Score: 856 bits (2211), Expect = 1.02e-305
Identity = 492/668 (73.65%), Postives = 523/668 (78.29%), Query Frame = 0

Query: 43  SPSPPSPDANSDTSPPPSPENDDSSP------PPPTSDN-SPSPKSPSDGSSDD------ 102
           SPSPPSPDANSD SPPP P NDDSS       P P+SD+ S  PKS SDG S D      
Sbjct: 37  SPSPPSPDANSDYSPPPPPTNDDSSSSSSSSSPAPSSDSGSQPPKSSSDGGSSDGGSSNG 96

Query: 103 --------------------------------HKSHPAPKEGGS---------------- 162
                                           + S+ +  +GGS                
Sbjct: 97  GSSDRGSSNNDSSNRGSSNGGSSDGGSNRSSGNDSNGSSSDGGSDRSSGNGKNDSPSSSK 156

Query: 163 --SPLTSPAA--HSPPSRSSSAGDEFSPPALPEGKASPRSPAKQQGTPSSEKDSSPS-EI 222
             S  +SP     S PSRSS      SPP   EGK+S RS   + GT      S PS ++
Sbjct: 157 DGSSRSSPFVPDQSSPSRSSPQSGALSPPI--EGKSSSRSSGDKHGT------SEPSLDV 216

Query: 223 NPGIIIGVAVGVGVFIIVLISVIAVCSKKKKRKRDPMPYYGDHGHGGPKGGDYYNSSLQQ 282
           NPGIIIGVAVGVGVFII+LI+++AVCSKKKKRK DPM YY DHGHG PKGGDYYNSS QQ
Sbjct: 217 NPGIIIGVAVGVGVFIILLIAIVAVCSKKKKRKGDPMQYY-DHGHG-PKGGDYYNSS-QQ 276

Query: 283 QQNWHNGMHGPGTDQFGRPPVSGSPAGTGWP----MSGENMTTGSSYAGPPLPPPSPSIA 342
             NW NGMHGPGTDQFGRPP SGS AG+GWP    MSG+   +G+   GPPLPPPSPSIA
Sbjct: 277 PTNWQNGMHGPGTDQFGRPPPSGS-AGSGWPSPPPMSGDMTMSGNYTGGPPLPPPSPSIA 336

Query: 343 FGFNKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGERE 402
           F F+KSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGERE
Sbjct: 337 FAFSKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKGVLPNGKEVAVKSLKVGSGQGERE 396

Query: 403 FMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTMEHHLHAKGLPVMDWPARLR 462
           FMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNT+EHHLH KGLPVMDWPARLR
Sbjct: 397 FMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVPNNTLEHHLHGKGLPVMDWPARLR 456

Query: 463 IAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSTDNHTHVSTRV 522
           I IGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLS+DNHTHVSTRV
Sbjct: 457 IGIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFEAMVADFGLAKLSSDNHTHVSTRV 516

Query: 523 MGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHTMEDSLVDWARPLMTR 582
           MGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTH MEDSLVDWARPLM R
Sbjct: 517 MGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGKRPVDPTHAMEDSLVDWARPLMNR 576

Query: 583 ALMEGIYDELVDIRLEREFNTQEMARMVACAAASIRHSARKRPKMSQVVRALEGDVSLDD 638
           AL EGIYD+LVD+RLE EFNTQEMARMVACAAA+IRHSARKRPKMSQ+VRALEGDVSLDD
Sbjct: 577 ALGEGIYDDLVDMRLENEFNTQEMARMVACAAAAIRHSARKRPKMSQIVRALEGDVSLDD 636

BLAST of Cucsat.G11788 vs. ExPASy TrEMBL
Match: A0A6J1FR50 (proline-rich receptor-like protein kinase PERK4 OS=Cucurbita moschata OX=3662 GN=LOC111447691 PE=4 SV=1)

HSP 1 Score: 788 bits (2034), Expect = 1.66e-279
Identity = 427/569 (75.04%), Postives = 467/569 (82.07%), Query Frame = 0

Query: 73  SDNSPSPKSPSDGSS-DDHKSHPAPKEGGSSPLTSPAAHSPPSRSSSAG-DEFSPPALPE 132
           SD SP+PKS  D       KS  AP    S+P+   A+ SP  RSSS+  D  +     E
Sbjct: 105 SDESPAPKSSKDSKDRKSGKSSKAPSPRSSTPI---ASKSPSPRSSSSSKDALTSGDRTE 164

Query: 133 GKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVLISVIAVCSKKKKRKR 192
           GK+S +S  K     S+  DS  S+++PG+IIGVAVGVG+FII+LIS+IA+CSKK+KRK 
Sbjct: 165 GKSSHKSSHKS----SNNDDSGSSDVSPGVIIGVAVGVGLFIILLISLIALCSKKRKRKE 224

Query: 193 DPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRPPVSGSPAGTGWP-MS 252
           DPM YY      GPKGG+YYN S  Q+ NWHN   G            G P   GWP MS
Sbjct: 225 DPMSYYSGDNANGPKGGNYYNGS--QKPNWHNNNAG------------GGPGVNGWPPMS 284

Query: 253 GENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKG 312
            ENMTT  S+ GPPLPPPSPS+AFGF+KSTFTYDELAAATGGFAHGNLLGQGGFGYVHKG
Sbjct: 285 SENMTTSGSFTGPPLPPPSPSLAFGFSKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKG 344

Query: 313 VLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVP 372
           VLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVP
Sbjct: 345 VLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVP 404

Query: 373 NNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFE 432
           NNT+EHHLH K LPVMDWP RLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFE
Sbjct: 405 NNTLEHHLHGKDLPVMDWPTRLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFE 464

Query: 433 AMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGK 492
           AMVADFGLAKLS+D +THVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGK
Sbjct: 465 AMVADFGLAKLSSDTNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGK 524

Query: 493 RPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMVACAAASIRH 552
           RPVDP H MEDSLVDWAR +++  + E +Y+ LVD RL +EFNTQEM RMV+CAAASIRH
Sbjct: 525 RPVDPHHVMEDSLVDWARAIISGEMGEDVYEVLVDKRLGKEFNTQEMGRMVSCAAASIRH 584

Query: 553 SARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHADMSKFRKIAL 612
           SARKRPKMSQVVRALEGD SLDDLN+G RPGQSSI+S   SDYDS+AY ADMS+FRK+AL
Sbjct: 585 SARKRPKMSQVVRALEGDYSLDDLNDGIRPGQSSIYSVAGSDYDSNAYQADMSRFRKLAL 644

Query: 613 SDDSSELATSSNDSREMNHPGPGTQRPLF 638
           SDDSSELATSSNDSRE+NHPG  TQRPLF
Sbjct: 645 SDDSSELATSSNDSREINHPG--TQRPLF 650

BLAST of Cucsat.G11788 vs. ExPASy TrEMBL
Match: A0A6J1IU50 (proline-rich receptor-like protein kinase PERK4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479341 PE=4 SV=1)

HSP 1 Score: 785 bits (2028), Expect = 3.55e-279
Identity = 424/569 (74.52%), Postives = 468/569 (82.25%), Query Frame = 0

Query: 74  DNSPSPKSPSDGSSDDH--KSHPAPKEGGSSPLTSPAAHSPPSRSSSAG-DEFSPPALPE 133
           D SP+PKS  D S D +  KS  AP    S+P+   A+ SP  RSSS+  D  +     E
Sbjct: 67  DESPAPKSSKDSSKDTNSGKSSKAPSPRSSTPI---ASKSPSPRSSSSSKDALTSGDRTE 126

Query: 134 GKASPRSPAKQQGTPSSEKDSSPSEINPGIIIGVAVGVGVFIIVLISVIAVCSKKKKRKR 193
           GK+S +S  K     S+  DS  S+++PG+IIGVAVGVG+FII+LIS+IA+CSKK+KRK 
Sbjct: 127 GKSSHKSSHKS----SNNDDSGSSDVSPGVIIGVAVGVGLFIILLISLIALCSKKRKRKE 186

Query: 194 DPMPYYGDHGHGGPKGGDYYNSSLQQQQNWHNGMHGPGTDQFGRPPVSGSPAGTGWP-MS 253
           DPM YY      GPKGG+YYN S  Q+ NWH   +             G P G GWP MS
Sbjct: 187 DPMSYYSGDNANGPKGGNYYNGS--QKPNWHINNN-----------AGGGPGGNGWPPMS 246

Query: 254 GENMTTGSSYAGPPLPPPSPSIAFGFNKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKG 313
            ENMTT  S+ GPPLPPPSPS+AFGF+KSTFTYDELAAATGGFAHGNLLGQGGFGYVHKG
Sbjct: 247 SENMTTSGSFTGPPLPPPSPSLAFGFSKSTFTYDELAAATGGFAHGNLLGQGGFGYVHKG 306

Query: 314 VLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVP 373
           VLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVP
Sbjct: 307 VLPNGKEVAVKSLKVGSGQGEREFMAEVEIISRVHHRHLVSLVGFCIAGGQRMLVYEFVP 366

Query: 374 NNTMEHHLHAKGLPVMDWPARLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFE 433
           NNT+EHHLH K LPVMDWP RLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFE
Sbjct: 367 NNTLEHHLHGKDLPVMDWPTRLRIAIGSAKGLAYLHEDCHPKIIHRDIKSANILIDANFE 426

Query: 434 AMVADFGLAKLSTDNHTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGK 493
           AMVADFGLAKLS+D +THVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFG+MLLELITGK
Sbjct: 427 AMVADFGLAKLSSDTNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGIMLLELITGK 486

Query: 494 RPVDPTHTMEDSLVDWARPLMTRALMEGIYDELVDIRLEREFNTQEMARMVACAAASIRH 553
           RPVD  + MEDSLVDWAR +++  + E +Y+ LVD RL +EFNTQEM RMV+CAAASIRH
Sbjct: 487 RPVDHNNVMEDSLVDWARAIISGEMGEDVYEVLVDKRLGKEFNTQEMGRMVSCAAASIRH 546

Query: 554 SARKRPKMSQVVRALEGDVSLDDLNEGTRPGQSSIFSATSSDYDSSAYHADMSKFRKIAL 613
           SARKRPKMSQVVRALEGD SLDDLN+G RPGQSSI+S   SDYDS+AY ADMS+FRK+AL
Sbjct: 547 SARKRPKMSQVVRALEGDYSLDDLNDGIRPGQSSIYSVAGSDYDSNAYQADMSRFRKLAL 606

Query: 614 SDDSSELATSSNDSREMNHPGPGTQRPLF 638
           SDDSSELATSSNDSRE+NHPG  TQRPLF
Sbjct: 607 SDDSSELATSSNDSREINHPG--TQRPLF 613

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZNQ81.8e-17258.76Proline-rich receptor-like protein kinase PERK4 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9XI962.0e-16353.85Proline-rich receptor-like protein kinase PERK7 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LS951.5e-15853.53Putative proline-rich receptor-like protein kinase PERK6 OS=Arabidopsis thaliana... [more]
Q8GX231.8e-15653.38Proline-rich receptor-like protein kinase PERK5 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LV484.4e-15554.28Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
XP_004149167.10.0100.00proline-rich receptor-like protein kinase PERK4 [Cucumis sativus] >KGN53256.1 hy... [more]
XP_008453734.10.091.01PREDICTED: proline-rich receptor-like protein kinase PERK4 [Cucumis melo][more]
XP_038877288.10.084.13proline-rich receptor-like protein kinase PERK4 [Benincasa hispida][more]
XP_022136397.12.11e-30573.65proline-rich receptor-like protein kinase PERK4 [Momordica charantia] >XP_022136... [more]
KAG6600609.11.23e-28173.91Proline-rich receptor-like protein kinase PERK4, partial [Cucurbita argyrosperma... [more]
Match NameE-valueIdentityDescription
A0A0A0KZE10.0100.00Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038... [more]
A0A1S3BX250.091.01proline-rich receptor-like protein kinase PERK4 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1C7H01.02e-30573.65proline-rich receptor-like protein kinase PERK4 OS=Momordica charantia OX=3673 G... [more]
A0A6J1FR501.66e-27975.04proline-rich receptor-like protein kinase PERK4 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1IU503.55e-27974.52proline-rich receptor-like protein kinase PERK4 isoform X2 OS=Cucurbita maxima O... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 292..553
e-value: 1.1E-32
score: 124.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 292..570
score: 35.551968
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 295..563
e-value: 4.5E-46
score: 157.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 268..367
e-value: 4.3E-34
score: 118.7
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 368..576
e-value: 2.3E-60
score: 205.5
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 296..492
e-value: 1.5E-26
score: 90.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 221..258
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 137..154
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 104..121
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 612..638
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 187..209
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 612..628
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..77
NoneNo IPR availablePANTHERPTHR47982:SF6PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4coord: 26..616
NoneNo IPR availablePANTHERPTHR47982PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4coord: 26..616
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 298..566
e-value: 1.16433E-104
score: 316.522
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 298..320
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 412..424
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 266..564

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G11788.T1Cucsat.G11788.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity