Cucsat.G11409 (gene) Cucumber (B10) v3

BLAST of Cucsat.G11409 vs. ExPASy Swiss-Prot
Match: Q9LV32 (DNA-directed RNA polymerase D subunit 2b OS=Arabidopsis thaliana OX=3702 GN=NRPD2b PE=2 SV=1)

HSP 1 Score: 1453.7 bits (3762), Expect = 0.0e+00
Identity = 703/1014 (69.33%), Postives = 834/1014 (82.25%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRDCE-----FDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTV 60
            MV S LC   ++ K +C+     FD+GGYF+IKGAEK+FIAQEQ+C +RLWISN   WTV
Sbjct: 125  MVKSVLCKTSEKGKENCKKGNCAFDQGGYFVIKGAEKVFIAQEQMCTKRLWISN-SPWTV 184

Query: 61   AYRSEVKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDL 120
            ++RSE KRNR I+RL EN K+ED K  EKVL VYFLSTE+PVW+LFFALGVSSDKE +DL
Sbjct: 185  SFRSETKRNRFIVRLSENEKAEDYKIMEKVLTVYFLSTEIPVWLLFFALGVSSDKEAMDL 244

Query: 121  IDYGRDDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLN 180
            I +  DD ++ N L AS+ E   D   + FR G  ALT+++++I+ T FPPA+ ++DCL 
Sbjct: 245  IAFDGDDASITNSLIASIHEA--DAVCEAFRCGNNALTYVEHQIKSTKFPPAESVDDCLR 304

Query: 181  LYLFASLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHI 240
            LYLF  L+G K+K  FLGYMVKCLL AY G+RKC+NRD FRNKR ELA ELLERE++VH+
Sbjct: 305  LYLFPCLQGLKKKARFLGYMVKCLLSAYAGKRKCENRDSFRNKRIELAGELLEREIRVHL 364

Query: 241  AHARRRMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVV 300
            AHARR+M +A+Q+ L GD  + PIEHYLDAS+ITNGL+RAFSTGAW+H F++MER+SGVV
Sbjct: 365  AHARRKMTRAMQKQLSGDGDLKPIEHYLDASVITNGLNRAFSTGAWSHPFRKMERVSGVV 424

Query: 301  ATLGRANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLS 360
            A LGRANPLQT+ +LRRTRQQV YTGKVGDAR+PHPSHWGRVCFLSTPDGENCGL+KN+S
Sbjct: 425  ANLGRANPLQTLIDLRRTRQQVLYTGKVGDARHPHPSHWGRVCFLSTPDGENCGLVKNMS 484

Query: 361  GTGLVSLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVR 420
              GLVS    +S+   LF CGME L+++TST  CGK+++ L+G+WVG+C DS SFV  ++
Sbjct: 485  LLGLVSTQGLESVVEMLFTCGMEELMNDTSTPLCGKHKVLLNGDWVGLCADSESFVGELK 544

Query: 421  RKRRRNPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLD 480
             +RR++    ++E+KRD+   EVRIF+DAGR+LRPLLVVENL+++ + K   Y F+ LLD
Sbjct: 545  SRRRQSELPLEMEIKRDKDDNEVRIFTDAGRLLRPLLVVENLHKLKQDKPTQYPFKHLLD 604

Query: 481  KGIIELIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHAR 540
            +GI+ELIG EEEEDC  AW IK L+++     Y+HCELD+SFLLG+SC +VPFANHDH +
Sbjct: 605  QGILELIGIEEEEDCTTAWGIKQLLKE--PKNYTHCELDLSFLLGVSCAIVPFANHDHGK 664

Query: 541  RALFQSQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGI 600
            R L+QSQKH  QAIGFS TN N R DTLS QL YPQ+PLF+T+ ++C             
Sbjct: 665  RVLYQSQKHCQQAIGFSSTNPNIRCDTLSQQLFYPQKPLFKTLASEC------------- 724

Query: 601  LPKPEFYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEK 660
            L K   +NGQNAIVAVNVHLGYNQEDS+VMN+ASLERGMFRSE IRSYKAEVD K+ SEK
Sbjct: 725  LEKEVLFNGQNAIVAVNVHLGYNQEDSIVMNKASLERGMFRSEQIRSYKAEVDTKD-SEK 784

Query: 661  RRKSDDAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTE 720
            R+K D+ + FGKT SKIG+VDSL+DDGFP+IGAN+ +GDIVIGRC ESGADHSIKLKHTE
Sbjct: 785  RKKMDELVQFGKTYSKIGKVDSLEDDGFPFIGANMSTGDIVIGRCTESGADHSIKLKHTE 844

Query: 721  KGMVQKVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQG 780
            +G+VQKVVLSSND+GKN+A VSLRQVRSPCLGDKFSSMHGQKGVLG+LE Q+NFPFTIQG
Sbjct: 845  RGIVQKVVLSSNDEGKNFAAVSLRQVRSPCLGDKFSSMHGQKGVLGYLEEQQNFPFTIQG 904

Query: 781  IVPDIVINPHAFPSRQTPGQLLEAALGKGIAA------GGSLKY------ATPFSTPSVD 840
            IVPDIVINPHAFPSRQTPGQLLEAAL KGIA       G S  Y      ATPFSTP V 
Sbjct: 905  IVPDIVINPHAFPSRQTPGQLLEAALSKGIACPIQKKEGSSAAYTKLTRHATPFSTPGVT 964

Query: 841  AITDQLHRAGFSRWGSERVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHP 900
             IT+QLHRAGFSRWG+ERVYNGR+GEMMRSLIFMGPTFYQRL+HM+E+KVKFRNTGPVHP
Sbjct: 965  EITEQLHRAGFSRWGNERVYNGRSGEMMRSLIFMGPTFYQRLVHMSENKVKFRNTGPVHP 1024

Query: 901  LTRQPVADRKRFGGIKFGEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVI 960
            LTRQPVADRKRFGGI+FGEMERDCLIAHGASANLHERLFTLSDSSQMH+C+KCK  A+VI
Sbjct: 1025 LTRQPVADRKRFGGIRFGEMERDCLIAHGASANLHERLFTLSDSSQMHICRKCKTYANVI 1084

Query: 961  QRSVAGGRKMRGPYCRVCESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 998
            +R+ + GRK+RGPYCRVC S D +VRV VPYGAKLLCQELFSMGI L F+T+LC
Sbjct: 1085 ERTPSSGRKIRGPYCRVCASSDHVVRVYVPYGAKLLCQELFSMGITLNFDTKLC 1119

BLAST of Cucsat.G11409 vs. ExPASy Swiss-Prot
Match: Q9LK40 (DNA-directed RNA polymerases IV and V subunit 2 OS=Arabidopsis thaliana OX=3702 GN=NRPD2 PE=1 SV=1)

HSP 1 Score: 1450.6 bits (3754), Expect = 0.0e+00
Identity = 703/1013 (69.40%), Postives = 829/1013 (81.84%), Query Frame = 0

Query: 1    MVNSDLCWMKDE-----QKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTV 60
            MV S LC   ++     +K DC FD+GGYF+IKGAEK+FIAQEQ+C +RLWISN   WTV
Sbjct: 179  MVKSILCKTSEKGKENCKKGDCAFDQGGYFVIKGAEKVFIAQEQMCTKRLWISN-SPWTV 238

Query: 61   AYRSEVKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDL 120
            ++RSE KRNR I+RL EN K+ED K +EKVL VYFLSTE+PVW+LFFALGVSSDKE +DL
Sbjct: 239  SFRSENKRNRFIVRLSENEKAEDYKRREKVLTVYFLSTEIPVWLLFFALGVSSDKEAMDL 298

Query: 121  IDYGRDDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLN 180
            I +  DD ++ N L AS+     D   + FR G  ALT+++ +I+ T FPPA+ +++CL+
Sbjct: 299  IAFDGDDASITNSLIASIHVA--DAVCEAFRCGNNALTYVEQQIKSTKFPPAESVDECLH 358

Query: 181  LYLFASLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHI 240
            LYLF  L+  K+K  FLGYMVKCLL +Y G+RKC+NRD FRNKR ELA ELLERE++VH+
Sbjct: 359  LYLFPGLQSLKKKARFLGYMVKCLLNSYAGKRKCENRDSFRNKRIELAGELLEREIRVHL 418

Query: 241  AHARRRMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVV 300
            AHARR+M +A+Q+ L GD  + PIEHYLDAS+ITNGLSRAFSTGAW+H F++MER+SGVV
Sbjct: 419  AHARRKMTRAMQKHLSGDGDLKPIEHYLDASVITNGLSRAFSTGAWSHPFRKMERVSGVV 478

Query: 301  ATLGRANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLS 360
            A LGRANPLQT+ +LRRTRQQV YTGKVGDARYPHPSHWGRVCFLSTPDGENCGL+KN+S
Sbjct: 479  ANLGRANPLQTLIDLRRTRQQVLYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLVKNMS 538

Query: 361  GTGLVSLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVR 420
              GLVS  + +S+   LF CGME L+D+T T   GK+++ L+G+WVG+C DS SFV  ++
Sbjct: 539  LLGLVSTQSLESVVEKLFACGMEELMDDTCTPLFGKHKVLLNGDWVGLCADSESFVAELK 598

Query: 421  RKRRRNPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLD 480
             +RR++    ++E+KRD+   EVRIF+DAGR+LRPLLVVENL ++ + K   Y F  LLD
Sbjct: 599  SRRRQSELPREMEIKRDKDDNEVRIFTDAGRLLRPLLVVENLQKLKQEKPSQYPFDHLLD 658

Query: 481  KGIIELIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHAR 540
             GI+ELIG EEEEDC  AW IK L+++     Y+HCELD+SFLLG+SC +VPFANHDH R
Sbjct: 659  HGILELIGIEEEEDCNTAWGIKQLLKEPKI--YTHCELDLSFLLGVSCAVVPFANHDHGR 718

Query: 541  RALFQSQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGI 600
            R L+QSQKH  QAIGFS TN N R DTLS QL YPQ+PLF+T+ ++C             
Sbjct: 719  RVLYQSQKHCQQAIGFSSTNPNIRCDTLSQQLFYPQKPLFKTLASEC------------- 778

Query: 601  LPKPEFYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEK 660
            L K   +NGQNAIVAVNVHLGYNQEDS+VMN+ASLERGMFRSE IRSYKAEVD K+ SEK
Sbjct: 779  LKKEVLFNGQNAIVAVNVHLGYNQEDSIVMNKASLERGMFRSEQIRSYKAEVDAKD-SEK 838

Query: 661  RRKSDDAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTE 720
            R+K D+ + FGKT SKIG+VDSL+DDGFP+IGAN+ +GDIVIGRC ESGADHSIKLKHTE
Sbjct: 839  RKKMDELVQFGKTHSKIGKVDSLEDDGFPFIGANMSTGDIVIGRCTESGADHSIKLKHTE 898

Query: 721  KGMVQKVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQG 780
            +G+VQKVVLSSND+GKN+A VSLRQVRSPCLGDKFSSMHGQKGVLG+LE Q+NFPFTIQG
Sbjct: 899  RGIVQKVVLSSNDEGKNFAAVSLRQVRSPCLGDKFSSMHGQKGVLGYLEEQQNFPFTIQG 958

Query: 781  IVPDIVINPHAFPSRQTPGQLLEAALGKGIAA-----GGSLKY------ATPFSTPSVDA 840
            IVPDIVINPHAFPSRQTPGQLLEAAL KGIA      G S  Y      ATPFSTP V  
Sbjct: 959  IVPDIVINPHAFPSRQTPGQLLEAALSKGIACPIQKEGSSAAYTKLTRHATPFSTPGVTE 1018

Query: 841  ITDQLHRAGFSRWGSERVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPL 900
            IT+QLHRAGFSRWG+ERVYNGR+GEMMRS+IFMGPTFYQRL+HM+EDKVKFRNTGPVHPL
Sbjct: 1019 ITEQLHRAGFSRWGNERVYNGRSGEMMRSMIFMGPTFYQRLVHMSEDKVKFRNTGPVHPL 1078

Query: 901  TRQPVADRKRFGGIKFGEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQ 960
            TRQPVADRKRFGGIKFGEMERDCLIAHGASANLHERLFTLSDSSQMH+C+KCK  A+VI+
Sbjct: 1079 TRQPVADRKRFGGIKFGEMERDCLIAHGASANLHERLFTLSDSSQMHICRKCKTYANVIE 1138

Query: 961  RSVAGGRKMRGPYCRVCESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 998
            R+ + GRK+RGPYCRVC S D +VRV VPYGAKLLCQELFSMGI L F+T+LC
Sbjct: 1139 RTPSSGRKIRGPYCRVCVSSDHVVRVYVPYGAKLLCQELFSMGITLNFDTKLC 1172

BLAST of Cucsat.G11409 vs. ExPASy Swiss-Prot
Match: P38420 (DNA-directed RNA polymerase II subunit 2 OS=Arabidopsis thaliana OX=3702 GN=NRPB2 PE=1 SV=2)

HSP 1 Score: 617.1 bits (1590), Expect = 3.5e-175
Identity = 378/1039 (36.38%), Postives = 570/1039 (54.86%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRD------CEFDRGGYFLIKGAEKIFIAQEQICLRRLWI-SNVQGW 60
            M+ S  C +    ++D      C +D+GGYF+I G+EK+ IAQE++    +++    Q  
Sbjct: 161  MLRSSYCTLFQNSEKDLTELGECPYDQGGYFIINGSEKVLIAQEKMSTNHVYVFKKRQPN 220

Query: 61   TVAYRSEVK-----RNR----LIIRLVENSKSEDLKSKEKV-LNVYFLSTEVPVWILFFA 120
              AY  EV+     +NR    + +R++  + ++   S + +   + ++ TE+P+ I+F A
Sbjct: 221  KYAYVGEVRSMAENQNRPPSTMFVRMLARASAKGGSSGQYIRCTLPYIRTEIPIIIVFRA 280

Query: 121  LGVSSDKEIVDLIDYGRDDPTVLNILFASVRE---VDNDDKWKDFRRGKRALTFLDNEIR 180
            LG  +DK+I++ I Y   D  ++ +L  S+ E   + N     D+  GKR  T    + +
Sbjct: 281  LGFVADKDILEHICYDFADTQMMELLRPSLEEAFVIQNQLVALDY-IGKRGATVGVTKEK 340

Query: 181  KTSFPPADKIEDCLNLYLFASLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRF 240
            +  +   D ++  +  ++        +K ++ GY++  LL    GRR  D+RD + NKR 
Sbjct: 341  RIKY-ARDILQKEMLPHVGIGEHCETKKAYYFGYIIHRLLLCALGRRPEDDRDHYGNKRL 400

Query: 241  ELAAELLERELKVHIAHARRRMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGA 300
            +LA  LL    ++      R +   +Q+ +   ++V+ ++  + A  IT+GL  + +TG 
Sbjct: 401  DLAGPLLGGLFRMLFRKLTRDVRSYVQKCVDNGKEVN-LQFAIKAKTITSGLKYSLATGN 460

Query: 301  WAHAFKRMERISGVVATLGRANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFL 360
            W  A     R +GV   L R     T++ LRR    +   GK+   R  H S WG +C  
Sbjct: 461  WGQANAAGTR-AGVSQVLNRLTYASTLSHLRRLNSPIGREGKLAKPRQLHNSQWGMMCPA 520

Query: 361  STPDGENCGLIKNLSGTGLVSLNTKK-SITPTLFRCGMENLVDNTSTSFCGKYRIFLDGE 420
             TP+G+ CGL+KNL+    +++ +    I   L   G EN  + + +      +IF++G 
Sbjct: 521  ETPEGQACGLVKNLALMVYITVGSAAYPILEFLEEWGTENFEEISPSVIPQATKIFVNGM 580

Query: 421  WVGVCEDSLSFVTNVRRKRRRNPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNR 480
            WVGV  D    V  +RR RRR     +V V RD +LKE+RI++D GR  RPL +V+N   
Sbjct: 581  WVGVHRDPDMLVKTLRRLRRRVDVNTEVGVVRDIRLKELRIYTDYGRCSRPLFIVDNQKL 640

Query: 481  IDKSK----------GENYTFQSLLDKGIIELIGTEEEEDCRVAWSIKHLM------EDE 540
            + K +           E   +  L+ KG IE I TEEEE   ++ +I  L+      E+ 
Sbjct: 641  LIKKRDIYALQQRESAEEDGWHHLVAKGFIEYIDTEEEETTMISMTISDLVQARLRPEEA 700

Query: 541  GTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQSQKHSNQAIGFSPTNSNFRVDTL 600
             T  Y+HCE+  S +LG+   ++PF +H+ + R  +QS     QA+G   TN  FR+DTL
Sbjct: 701  YTENYTHCEIHPSLILGVCASIIPFPDHNQSPRNTYQSAM-GKQAMGIYVTNYQFRMDTL 760

Query: 601  SHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPEFYNGQNAIVAVNVHLGYNQEDSL 660
            ++ L+YPQ+PL  T   + L        H   LP      G NAIVA++ + GYNQEDS+
Sbjct: 761  AYVLYYPQKPLVTTRAMEHL--------HFRQLPA-----GINAIVAISCYSGYNQEDSV 820

Query: 661  VMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSDDAINFGKTQS-KIGRVDSLDDDG 720
            +MN++S++RG FRS   RSY+ E   K+     ++     + G T   + G  D LDDDG
Sbjct: 821  IMNQSSIDRGFFRSLFFRSYRDE--EKKMGTLVKEDFGRPDRGSTMGMRHGSYDKLDDDG 880

Query: 721  FPYIGANLQSGDIVIGRCAE-------------SGADHSIKLKHTEKGMVQKVVLSSNDD 780
                G  +   D++IG+                +  DHSI L+H+E GMV +V+L++N D
Sbjct: 881  LAPPGTRVSGEDVIIGKTTPISQDEAQGQSSRYTRRDHSISLRHSETGMVDQVLLTTNAD 940

Query: 781  GKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDIVINPHAFPS 840
            G  +  V +R VR P +GDKFSS HGQKG +G   +QE+ P+TI+G+ PDI++NPHA PS
Sbjct: 941  GLRFVKVRVRSVRIPQIGDKFSSRHGQKGTVGMTYTQEDMPWTIEGVTPDIIVNPHAIPS 1000

Query: 841  RQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSERVYNGRTGE 900
            R T GQL+E  +GK  A  G    ATPF+  +VD I+  LH+ G+   G ER+YNG TG 
Sbjct: 1001 RMTIGQLIECIMGKVAAHMGKEGDATPFTDVTVDNISKALHKCGYQMRGFERMYNGHTGR 1060

Query: 901  MMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKFGEMERDCLI 960
             + ++IF+GPT+YQRL HM +DK+  R  GPV  LTRQP   R R GG++FGEMERDC+I
Sbjct: 1061 PLTAMIFLGPTYYQRLKHMVDDKIHSRGRGPVQILTRQPAEGRSRDGGLRFGEMERDCMI 1120

Query: 961  AHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRVCESFDDIVR 989
            AHGA+  L ERLF  SD+ ++HVC+ C  +A           K     CR C++  DIV+
Sbjct: 1121 AHGAAHFLKERLFDQSDAYRVHVCEVCGLIA-------IANLKKNSFECRGCKNKTDIVQ 1172

BLAST of Cucsat.G11409 vs. ExPASy Swiss-Prot
Match: Q42877 (DNA-directed RNA polymerase II subunit RPB2 OS=Solanum lycopersicum OX=4081 GN=RPB2 PE=2 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 3.3e-173
Identity = 374/1049 (35.65%), Postives = 566/1049 (53.96%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRD------CEFDRGGYFLIKGAEKIFIAQEQICLRRLWI-SNVQGW 60
            M+ S  C +    ++D      C  D+GGYF+I G+EK+ IAQE++    +++    Q  
Sbjct: 160  MLRSSYCTLYQNSEKDLTELGECPLDQGGYFIINGSEKVLIAQEKMSTNHVYVFKKRQPN 219

Query: 61   TVAYRSEVK-----RNR----LIIRLVENSKSEDLKSKEKV-LNVYFLSTEVPVWILFFA 120
              A+ +EV+     +NR    + +R++  + ++   S + +   + ++ TE+P+ I+F A
Sbjct: 220  KYAFVAEVRSMADTQNRPPSTMFVRMLSRTSAKGGSSGQYIRATLPYIRTEIPIIIVFRA 279

Query: 121  LGVSSDKEIVDLIDYGRDDPTVLNILFASVRE---VDNDDKWKDF--RRG-------KRA 180
            LG  +DK+I++ I Y  +D  ++ +L  S+ E   + N     D+  +RG       ++ 
Sbjct: 280  LGFVADKDILEHICYDFNDTQMMELLRPSLEEAFVIQNQQVALDYIGKRGATVGVTREKR 339

Query: 181  LTFLDNEIRKTSFPPADKIEDCLNLYLFASLKGSKQKCHFLGYMVKCLLQAYTGRRKCDN 240
            + +    ++K   P     E C             +K ++ GY++  LL    GRR  D+
Sbjct: 340  IKYAKEILQKEMLPHVGVGEYC-----------ETKKAYYFGYIIHRLLLCALGRRAEDD 399

Query: 241  RDDFRNKRFELAAELLERELKVHIAHARRRMEKALQRDLYGDRQVHPIEHYLDASIITNG 300
            RD + NKR +LA  LL    ++      R +   +Q+ +   + V+ ++  + A  IT+G
Sbjct: 400  RDHYGNKRLDLAGPLLGGLFRMLFRKLTRDVRGYVQKCVDNGKDVN-LQFAIKAKTITSG 459

Query: 301  LSRAFSTGAWAHAFKRMERISGVVATLGRANPLQTMAELRRTRQQVAYTGKVGDARYPHP 360
            L  + +TG W  A     R +GV   L R     T++ LRR    +   GK+   R  H 
Sbjct: 460  LKYSLATGNWGQANAAGTR-AGVSQVLNRLTYASTLSHLRRLNSPIGREGKLAKPRQLHN 519

Query: 361  SHWGRVCFLSTPDGENCGLIKNLSGTGLVSLNTKK-SITPTLFRCGMENLVDNTSTSFCG 420
            S WG +C   TP+G+ CGL+KNL+    +++ +    I   L   G EN  + +      
Sbjct: 520  SQWGMMCPAETPEGQACGLVKNLALMVYITVGSAAYPILEFLEEWGTENFEEISPAVIPQ 579

Query: 421  KYRIFLDGEWVGVCEDSLSFVTNVRRKRRRNPFLHQVEVKRDEQLKEVRIFSDAGRILRP 480
              +IF++G WVG+  D    V  +RR RRR     +V V RD +LKE+RI++D GR  RP
Sbjct: 580  ATKIFVNGTWVGIHRDPDMLVRTLRRLRRRVDVNTEVGVVRDIRLKELRIYTDYGRCSRP 639

Query: 481  LLVVENLNRIDKSKG----------ENYTFQSLLDKGIIELIGTEEEEDCRVAWSIKHLM 540
            L +VE    + K K           +   +  L+ KG IE I TEEEE   ++ +I  L+
Sbjct: 640  LFIVEKQRLMIKKKDIQTLQQRESPDEGGWHDLVAKGYIEYIDTEEEETTMISMTINDLV 699

Query: 541  E------DEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQSQKHSNQAIGFSPT 600
            +      D  +  Y+HCE+  S +LG+   ++PF +H+ + R  +QS     QA+G   T
Sbjct: 700  QARLNPGDAYSDTYTHCEIHPSLILGVCASIIPFPDHNQSPRNTYQSAM-GKQAMGIYVT 759

Query: 601  NSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPEFYNGQNAIVAVNVH 660
            N  FR+DTL++ L+YPQ+PL  T   + L        H   LP      G NAIVA++ +
Sbjct: 760  NYQFRMDTLAYVLYYPQKPLVTTRAMEHL--------HFRQLPA-----GINAIVAISCY 819

Query: 661  LGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKES--SEKRRKSDDAINFGKTQSKI 720
             GYNQEDS++MN++S++RG FRS   RSY+ E     +   E   + D A   G    + 
Sbjct: 820  SGYNQEDSVIMNQSSIDRGFFRSLFFRSYRDEEKKMGTLVKEDFGRPDRASTMG---MRH 879

Query: 721  GRVDSLDDDGFPYIGANLQSGDIVIGRCAE-------------SGADHSIKLKHTEKGMV 780
            G  D LDDDG    G  +   D++IG+                +  DHS  L+H+E GMV
Sbjct: 880  GSYDKLDDDGLAPPGTRVSGEDVIIGKTTPISQDDAQGQASRYTRKDHSTSLRHSETGMV 939

Query: 781  QKVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPD 840
             +V+L++N DG  +  V +R VR P +GDKFSS HGQKG +G   +QE+ P+T++GI PD
Sbjct: 940  DQVLLTTNADGLRFVKVRVRSVRIPQIGDKFSSRHGQKGTVGMTYTQEDMPWTVEGITPD 999

Query: 841  IVINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGS 900
            I++NPHA PSR T GQL+E  +GK  A  G    ATPF+  +VD I+  LH+ G+   G 
Sbjct: 1000 IIVNPHAIPSRMTIGQLIECIMGKVAAHMGKEGDATPFTDVTVDNISKALHKCGYQMRGF 1059

Query: 901  ERVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIK 960
            E +YNG TG  + ++IF+GPT+YQRL HM +DK+  R  GPV  LTRQP   R R GG++
Sbjct: 1060 ETMYNGHTGRRLSAMIFLGPTYYQRLKHMVDDKIHSRGRGPVQILTRQPAEGRSRDGGLR 1119

Query: 961  FGEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCR 989
            FGEMERDC+IAHGA+  L ERLF  SD+ ++HVC++C  +A           K     CR
Sbjct: 1120 FGEMERDCMIAHGAAHFLKERLFDQSDAYRVHVCERCGLIA-------IANLKKNSFECR 1171

BLAST of Cucsat.G11409 vs. ExPASy Swiss-Prot
Match: Q54J75 (DNA-directed RNA polymerase II subunit rpb2 OS=Dictyostelium discoideum OX=44689 GN=polr2b PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 4.3e-165
Identity = 364/1042 (34.93%), Postives = 558/1042 (53.55%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRD------CEFDRGGYFLIKGAEKIFIAQEQICLRRLWI-SNVQGW 60
            M+ S  C + +   RD      C FD+GGYF+I G+EK+ IAQE++    +++       
Sbjct: 159  MLRSQYCMLNEADDRDLTTMGECSFDQGGYFIINGSEKVLIAQEKMNNNHVYVFKKSPPS 218

Query: 61   TVAYRSEVK---------RNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFAL 120
              +Y +E++          + + ++++ N+    +  K     V ++  +VPV I+F AL
Sbjct: 219  KYSYVAEIRSCQETGSRPTSTMYLKMLHNTNKGPVGIK---ATVPYIKQDVPVIIVFRAL 278

Query: 121  GVSSDKEIVDLIDYGRDDPTVLNILFASVRE---VDNDDKWKDFRRGKRALTFLDNEIRK 180
            G  +DK+I++ I Y   D  +++++  S+ E   + + +   D+  GKR  T    E ++
Sbjct: 279  GFVADKDILEHICYDFKDAQMMDLMRPSLEESFVIQSQEVALDY-LGKRGSTMGTRE-QR 338

Query: 181  TSFPPADKIEDCLNLYLFASLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFE 240
              F      ++ L     A    +K K  + GY+   LL A   RR  D+RD F NKR +
Sbjct: 339  IKFAREVLQKEMLPHVSVAEFYETK-KAFYFGYITHRLLLASLERRPLDDRDHFANKRLD 398

Query: 241  LAAELLERELKVHIAHARRRMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAW 300
            LA  LL    +       + +   LQR +  D++       + +  IT+GL  + +TG W
Sbjct: 399  LAGPLLGTLFRQLFKKLTKDVRLYLQRCIDKDKE-FIASSAVKSKTITSGLKYSLATGNW 458

Query: 301  AHAFKRMERISGVVATLGRANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLS 360
              + K     SGV   L R     T++ LRR    +   GK+   R  H +HWG VC   
Sbjct: 459  GSS-KSGGTKSGVAQVLNRLTFASTLSHLRRLNTPIGREGKLAKPRQLHNTHWGIVCPSE 518

Query: 361  TPDGENCGLIKNLSGTGLVSL-NTKKSITPTLFRCGMENLVD-NTSTSFCGKYRIFLDGE 420
            TP+G+ CGL+KNL+    +S+ +  + I   L     ENL + +  +S     +IF++G 
Sbjct: 519  TPEGQACGLVKNLAMMSYISVGSASQPILEFLEEWTTENLEEISDLSSIFSATKIFVNGM 578

Query: 421  WVGVCEDSLSFVTNVRRKRRRNPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNR 480
            WVG+       ++ +R  RR      +V V RD + KE+R+++D GR  RPL VV++ N+
Sbjct: 579  WVGIHHQPDKLLSTLRLLRRCGDVSVEVSVVRDIREKELRLYTDPGRCCRPLFVVQD-NK 638

Query: 481  IDKSK--------GENYTFQSLLDKGIIELIGTEEEEDCRVAWSIKHLM---EDEGTTKY 540
            +   K         + Y +Q LL +GI+E I  EEEE   +A + + L+    ++    Y
Sbjct: 639  VQIKKQHINKLINNDEYKWQDLLSEGIVEYIDAEEEETVLIAMTPEDLVPMPNEQIVHTY 698

Query: 541  SHCELDMSFLLGLSCGLVPFANHDHARRALFQSQKHSNQAIGFSPTNSNFRVDTLSHQLH 600
            +HCE+  S +LG+ C ++PF +H+ + R  +Q+     QA+G   TN   R+DT++H L 
Sbjct: 699  THCEIHPSMILGICCSIIPFPDHNQSPRNTYQAAM-GKQAMGVYITNYQLRMDTMAHVLF 758

Query: 601  YPQRPLFRTMTADCLGTPGYLSSHAGILPKPEFYNGQNAIVAVNVHLGYNQEDSLVMNRA 660
            YPQ+PL  T + + L        H   LP      GQN  VA+  + GYNQEDS+++N++
Sbjct: 759  YPQKPLVTTRSMEYL--------HFRELPA-----GQNVCVAIACYSGYNQEDSVILNQS 818

Query: 661  SLERGMFRSEHIRSYKAEVDNKESSEKRRKSDDAINFGKTQSKIGRVDSLDDDGFPYIGA 720
            +++RG+FRS   R+YK E   K++S      +          + G  + +DDDG    G 
Sbjct: 819  AIDRGLFRSMFYRAYKDE-QKKQTSLMEEVFEKPERDTCAGMRHGSYEKIDDDGLVAPGV 878

Query: 721  NLQSGDIVIGRCA---------------ESGADHSIKLKHTEKGMVQKVVLSSNDDGKNY 780
             +   DI+IG+                  +  D S  L+ +E G+V +V+L+++ +G  +
Sbjct: 879  RVAGDDIIIGKTTPLPPSDDDLGPKTRRHTKRDSSTALRSSETGIVDQVILTTSGEGFKF 938

Query: 781  AVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDIVINPHAFPSRQTP 840
              V +R +R P +GDKFSS HGQKG  G    QE+ PFT++GIVPDI++NPHA PSR T 
Sbjct: 939  CKVRVRSMRVPQIGDKFSSRHGQKGTCGMAYRQEDLPFTVEGIVPDIIVNPHAIPSRMTI 998

Query: 841  GQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSERVYNGRTGEMMRS 900
            GQL+E  LGK  A+ G    ATPF+  +V+AI+  LH+ G+   G E +YNG TG  M +
Sbjct: 999  GQLIECLLGKVSASTGDEGDATPFTDVTVEAISQALHKIGYQMTGHEVMYNGHTGRRMDA 1058

Query: 901  LIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKFGEMERDCLIAHGA 960
             IF+GPT+YQRL HM +DK+  R+ GPV  LTRQPV  R R GG++FGEMERDC+I+HGA
Sbjct: 1059 QIFIGPTYYQRLKHMVDDKIHSRSRGPVQILTRQPVEGRSRDGGLRFGEMERDCMISHGA 1118

Query: 961  SANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRVCESFDDIVRVNVP 996
            +  L ERLF  SDS ++H+C  C  +A           K     CR C++   I ++ +P
Sbjct: 1119 AQFLKERLFDQSDSYRVHICDICGLIA-------IANLKKNSFECRRCKNKTQISQIRMP 1169

BLAST of Cucsat.G11409 vs. NCBI nr
Match: XP_031740252.1 (DNA-directed RNA polymerases IV and V subunit 2 [Cucumis sativus] >KGN55429.1 hypothetical protein Csa_012655 [Cucumis sativus])

HSP 1 Score: 2019 bits (5230), Expect = 0.0
Identity = 996/997 (99.90%), Postives = 996/997 (99.90%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 60
            MVNSDLCWMKD QKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE
Sbjct: 201  MVNSDLCWMKDGQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 260

Query: 61   VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 120
            VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR
Sbjct: 261  VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 320

Query: 121  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 180
            DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA
Sbjct: 321  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 380

Query: 181  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 240
            SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR
Sbjct: 381  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 440

Query: 241  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 300
            RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR
Sbjct: 441  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 500

Query: 301  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 360
            ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV
Sbjct: 501  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 560

Query: 361  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 420
            SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR
Sbjct: 561  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 620

Query: 421  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 480
            NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE
Sbjct: 621  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 680

Query: 481  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 540
            LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ
Sbjct: 681  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 740

Query: 541  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 600
            SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE
Sbjct: 741  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 800

Query: 601  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 660
            FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD
Sbjct: 801  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 860

Query: 661  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 720
            DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ
Sbjct: 861  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 920

Query: 721  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 780
            KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI
Sbjct: 921  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 980

Query: 781  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 840
            VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE
Sbjct: 981  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 1040

Query: 841  RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 900
            RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF
Sbjct: 1041 RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 1100

Query: 901  GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 960
            GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV
Sbjct: 1101 GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 1160

Query: 961  CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 997
            CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC
Sbjct: 1161 CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 1197

BLAST of Cucsat.G11409 vs. NCBI nr
Match: XP_008452849.1 (PREDICTED: DNA-directed RNA polymerases IV and V subunit 2-like [Cucumis melo] >KAA0064582.1 DNA-directed RNA polymerases IV and V subunit 2-like [Cucumis melo var. makuwa] >TYK20008.1 DNA-directed RNA polymerases IV and V subunit 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1986 bits (5145), Expect = 0.0
Identity = 980/997 (98.29%), Postives = 983/997 (98.60%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 60
            MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE
Sbjct: 201  MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 260

Query: 61   VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 120
            VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR
Sbjct: 261  VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 320

Query: 121  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 180
            DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPA+KIEDCLNLYLFA
Sbjct: 321  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPAEKIEDCLNLYLFA 380

Query: 181  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 240
            SLKG KQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR
Sbjct: 381  SLKGLKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 440

Query: 241  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 300
            RMEKALQRDLYGDRQV PIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR
Sbjct: 441  RMEKALQRDLYGDRQVRPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 500

Query: 301  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 360
            ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV
Sbjct: 501  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 560

Query: 361  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 420
            SLNT K I PTLF CGMENL DNTSTS CGKYRIFLDGEWVGVCEDSLSFVT VRRKRRR
Sbjct: 561  SLNTTKPIIPTLFCCGMENLADNTSTSLCGKYRIFLDGEWVGVCEDSLSFVTKVRRKRRR 620

Query: 421  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 480
            NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE
Sbjct: 621  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 680

Query: 481  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 540
            L+GTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ
Sbjct: 681  LVGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 740

Query: 541  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 600
            SQKHSNQAIGFS TNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSH GILPKPE
Sbjct: 741  SQKHSNQAIGFSTTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHTGILPKPE 800

Query: 601  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 660
            FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEH RSYKAEVDNKESSEKRRKSD
Sbjct: 801  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHTRSYKAEVDNKESSEKRRKSD 860

Query: 661  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 720
            D INFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ
Sbjct: 861  DTINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 920

Query: 721  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 780
            KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI
Sbjct: 921  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 980

Query: 781  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 840
            VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE
Sbjct: 981  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 1040

Query: 841  RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 900
            RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF
Sbjct: 1041 RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 1100

Query: 901  GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 960
            GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRK+RGPYCRV
Sbjct: 1101 GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKIRGPYCRV 1160

Query: 961  CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 997
            CES DDIVRVNVPYGAKLLCQELFSMGINLKFETQLC
Sbjct: 1161 CESVDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 1197

BLAST of Cucsat.G11409 vs. NCBI nr
Match: XP_038897771.1 (DNA-directed RNA polymerases IV and V subunit 2-like [Benincasa hispida])

HSP 1 Score: 1955 bits (5064), Expect = 0.0
Identity = 961/997 (96.39%), Postives = 978/997 (98.09%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 60
            MVNSDLCWMK+EQKRDCEFD GGYFLIKGAEKIFIAQEQICLRRLWISN+QGWTVAYRSE
Sbjct: 198  MVNSDLCWMKEEQKRDCEFDHGGYFLIKGAEKIFIAQEQICLRRLWISNIQGWTVAYRSE 257

Query: 61   VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 120
            VKRNRLIIRLVENSKSE+LKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR
Sbjct: 258  VKRNRLIIRLVENSKSEELKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 317

Query: 121  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 180
            DDP+VLNILFASVREVDNDDKWKDFRRGKRAL FLDNE+RKTSFPPA+KIEDCLNLYLFA
Sbjct: 318  DDPSVLNILFASVREVDNDDKWKDFRRGKRALNFLDNEVRKTSFPPAEKIEDCLNLYLFA 377

Query: 181  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 240
            SLKG KQKC FLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELA ELLERE+KVHIAHARR
Sbjct: 378  SLKGLKQKCRFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAGELLEREIKVHIAHARR 437

Query: 241  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 300
            RMEKALQRDLYGDR V PIEHYLDASIITNGLSRAFSTGAWAH+FKRMERISGVVATLGR
Sbjct: 438  RMEKALQRDLYGDRDVRPIEHYLDASIITNGLSRAFSTGAWAHSFKRMERISGVVATLGR 497

Query: 301  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 360
            ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCF+STPDGENCGLIKNLS TGLV
Sbjct: 498  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFISTPDGENCGLIKNLSVTGLV 557

Query: 361  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 420
            SLNT K I PTLFRCGMENLVD+TSTS  GKY+IFLDGEWVGVCEDSLSFVT VRRKRRR
Sbjct: 558  SLNTLKPIVPTLFRCGMENLVDDTSTSLSGKYKIFLDGEWVGVCEDSLSFVTKVRRKRRR 617

Query: 421  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 480
            NP LHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLD+GIIE
Sbjct: 618  NPLLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDRGIIE 677

Query: 481  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 540
            LIGTEEEEDCR AWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ
Sbjct: 678  LIGTEEEEDCRTAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 737

Query: 541  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 600
            SQKHSNQAIGFS TNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSH GILPKPE
Sbjct: 738  SQKHSNQAIGFSTTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHTGILPKPE 797

Query: 601  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 660
            FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD
Sbjct: 798  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 857

Query: 661  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 720
            DAINFGKTQSKIGRVDSLD+DGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ
Sbjct: 858  DAINFGKTQSKIGRVDSLDEDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 917

Query: 721  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 780
            KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI
Sbjct: 918  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 977

Query: 781  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 840
            VINPHAFPSRQTPGQLLEAALGKGIA+GGSLKYATPFSTPSVDAIT+QLHRAGFSRWGSE
Sbjct: 978  VINPHAFPSRQTPGQLLEAALGKGIASGGSLKYATPFSTPSVDAITEQLHRAGFSRWGSE 1037

Query: 841  RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 900
            RVYNGRTGEMM+SLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF
Sbjct: 1038 RVYNGRTGEMMQSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 1097

Query: 901  GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 960
            GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRK+RGPYCRV
Sbjct: 1098 GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKIRGPYCRV 1157

Query: 961  CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 997
            CES DDIVRVNVPYGAKLLCQELFSMGINLKFETQLC
Sbjct: 1158 CESVDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 1194

BLAST of Cucsat.G11409 vs. NCBI nr
Match: KAG7037651.1 (DNA-directed RNA polymerases IV and V subunit 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1927 bits (4991), Expect = 0.0
Identity = 946/997 (94.88%), Postives = 972/997 (97.49%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 60
            MVNSDLCWMKD+QKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISN QGWTVAYRSE
Sbjct: 197  MVNSDLCWMKDDQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNNQGWTVAYRSE 256

Query: 61   VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 120
            VKRNRLIIRLVENSKSE+LKSKEKVLNVYFLSTE+PVWILFFALGVSSD+EIVDLID+GR
Sbjct: 257  VKRNRLIIRLVENSKSEELKSKEKVLNVYFLSTEIPVWILFFALGVSSDREIVDLIDFGR 316

Query: 121  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 180
            +DPTVLNILFASVR VDNDDKWKDFRRGKRAL FL+NE++KTSFPPA+KIEDCLNLYLFA
Sbjct: 317  EDPTVLNILFASVRVVDNDDKWKDFRRGKRALNFLENEVKKTSFPPAEKIEDCLNLYLFA 376

Query: 181  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 240
            SLKG KQKC FLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELA ELLERE+KVHIAHARR
Sbjct: 377  SLKGLKQKCRFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAGELLEREIKVHIAHARR 436

Query: 241  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 300
            RMEKALQRDLYGDR V PIEHY DASIITNGLSRAFSTGAWAH+FKRMERISGVVATLGR
Sbjct: 437  RMEKALQRDLYGDRDVRPIEHYFDASIITNGLSRAFSTGAWAHSFKRMERISGVVATLGR 496

Query: 301  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 360
            ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLS TGLV
Sbjct: 497  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSITGLV 556

Query: 361  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 420
            SLNT K + PTLFRCGMEN+VD+TSTS  GK+RIFL+GEWVGVCEDSLSFVT VRRKRRR
Sbjct: 557  SLNTLKPVVPTLFRCGMENVVDDTSTSLSGKHRIFLNGEWVGVCEDSLSFVTKVRRKRRR 616

Query: 421  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 480
            N  LHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGE+YTFQSLLDKGIIE
Sbjct: 617  NLLLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGESYTFQSLLDKGIIE 676

Query: 481  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 540
            LIGTEEEEDCR AWSIKHL ED+GT+KY+HCELD SFLLGLSCGLVPFANHDHARRALFQ
Sbjct: 677  LIGTEEEEDCRTAWSIKHLTEDQGTSKYTHCELDTSFLLGLSCGLVPFANHDHARRALFQ 736

Query: 541  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 600
            SQKHSNQAIGFS TNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSH GILPKPE
Sbjct: 737  SQKHSNQAIGFSTTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHTGILPKPE 796

Query: 601  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 660
            FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEH RSYKAEVDNKESSEKRRKSD
Sbjct: 797  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHTRSYKAEVDNKESSEKRRKSD 856

Query: 661  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 720
            DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ
Sbjct: 857  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 916

Query: 721  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 780
            KVVLSSNDDGKN+AVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI
Sbjct: 917  KVVLSSNDDGKNFAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 976

Query: 781  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 840
            VINPHAFPSRQTPGQLLEAALGKGIA+GGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE
Sbjct: 977  VINPHAFPSRQTPGQLLEAALGKGIASGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 1036

Query: 841  RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 900
            RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF
Sbjct: 1037 RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 1096

Query: 901  GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 960
            GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVA+VIQRSVAGGRK+RGPYCRV
Sbjct: 1097 GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVANVIQRSVAGGRKIRGPYCRV 1156

Query: 961  CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 997
            CES DDIVRVNVPYGAKLLCQELFSMGINLKFETQ+C
Sbjct: 1157 CESVDDIVRVNVPYGAKLLCQELFSMGINLKFETQVC 1193

BLAST of Cucsat.G11409 vs. NCBI nr
Match: XP_023524029.1 (DNA-directed RNA polymerases IV and V subunit 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1925 bits (4987), Expect = 0.0
Identity = 945/997 (94.78%), Postives = 971/997 (97.39%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 60
            MVNSDLCWMKD+QKRDCEFD GGYFLIKGAEKIFIAQEQICLRRLWISN QGWTVAYRSE
Sbjct: 197  MVNSDLCWMKDDQKRDCEFDHGGYFLIKGAEKIFIAQEQICLRRLWISNNQGWTVAYRSE 256

Query: 61   VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 120
            VKRNRLIIRLVENSKSE+LKSKEKVLNVYFLSTE+PVWILFFALGVSSD+EIVDLID+GR
Sbjct: 257  VKRNRLIIRLVENSKSEELKSKEKVLNVYFLSTEIPVWILFFALGVSSDREIVDLIDFGR 316

Query: 121  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 180
            +DPTVLNILFASVR VDNDDKWKDFRRGKRAL FL+NE++KTSFPPA+KIEDCLNLYLFA
Sbjct: 317  EDPTVLNILFASVRVVDNDDKWKDFRRGKRALNFLENEVKKTSFPPAEKIEDCLNLYLFA 376

Query: 181  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 240
            SLKG KQKC FLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELA ELLERE+KVHIAHARR
Sbjct: 377  SLKGLKQKCRFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAGELLEREIKVHIAHARR 436

Query: 241  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 300
            RMEKALQRDLYGDR V PIEHY DASIITNGLSRAFSTGAWAH+FKRMERISGVVATLGR
Sbjct: 437  RMEKALQRDLYGDRDVRPIEHYFDASIITNGLSRAFSTGAWAHSFKRMERISGVVATLGR 496

Query: 301  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 360
            ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLS TGLV
Sbjct: 497  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSITGLV 556

Query: 361  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 420
            SLNT K + PTLFRCGMEN+VD+TSTS  GK+RIFL+GEWVGVCEDSLSFVT VRRKRRR
Sbjct: 557  SLNTLKPVVPTLFRCGMENMVDDTSTSLSGKHRIFLNGEWVGVCEDSLSFVTKVRRKRRR 616

Query: 421  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 480
            N  LHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGE+YTFQSLLDKGIIE
Sbjct: 617  NLLLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGESYTFQSLLDKGIIE 676

Query: 481  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 540
            LIGTEEEEDCR AWSIKHL ED+GT+KY+HCELD SFLLGLSCGLVPFANHDHARRALFQ
Sbjct: 677  LIGTEEEEDCRTAWSIKHLTEDQGTSKYTHCELDTSFLLGLSCGLVPFANHDHARRALFQ 736

Query: 541  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 600
            SQKHSNQAIGFS TNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSH GILPKPE
Sbjct: 737  SQKHSNQAIGFSTTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHTGILPKPE 796

Query: 601  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 660
            FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEH RSYKAEVDNKESSEKRRKSD
Sbjct: 797  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHTRSYKAEVDNKESSEKRRKSD 856

Query: 661  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 720
            DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ
Sbjct: 857  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 916

Query: 721  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 780
            KVVLSSNDDGKN+AVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI
Sbjct: 917  KVVLSSNDDGKNFAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 976

Query: 781  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 840
            VINPHAFPSRQTPGQLLEAALGKGIA+GGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE
Sbjct: 977  VINPHAFPSRQTPGQLLEAALGKGIASGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 1036

Query: 841  RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 900
            RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF
Sbjct: 1037 RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 1096

Query: 901  GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 960
            GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVA+VIQRSVAGGRK+RGPYCRV
Sbjct: 1097 GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVANVIQRSVAGGRKIRGPYCRV 1156

Query: 961  CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 997
            CES DDIVRVNVPYGAKLLCQELFSMGINLKFETQ+C
Sbjct: 1157 CESVDDIVRVNVPYGAKLLCQELFSMGINLKFETQVC 1193

BLAST of Cucsat.G11409 vs. ExPASy TrEMBL
Match: A0A0A0L319 (DNA-directed RNA polymerase subunit beta OS=Cucumis sativus OX=3659 GN=Csa_4G651950 PE=3 SV=1)

HSP 1 Score: 2019 bits (5230), Expect = 0.0
Identity = 996/997 (99.90%), Postives = 996/997 (99.90%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 60
            MVNSDLCWMKD QKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE
Sbjct: 201  MVNSDLCWMKDGQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 260

Query: 61   VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 120
            VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR
Sbjct: 261  VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 320

Query: 121  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 180
            DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA
Sbjct: 321  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 380

Query: 181  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 240
            SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR
Sbjct: 381  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 440

Query: 241  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 300
            RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR
Sbjct: 441  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 500

Query: 301  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 360
            ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV
Sbjct: 501  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 560

Query: 361  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 420
            SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR
Sbjct: 561  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 620

Query: 421  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 480
            NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE
Sbjct: 621  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 680

Query: 481  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 540
            LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ
Sbjct: 681  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 740

Query: 541  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 600
            SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE
Sbjct: 741  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 800

Query: 601  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 660
            FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD
Sbjct: 801  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 860

Query: 661  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 720
            DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ
Sbjct: 861  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 920

Query: 721  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 780
            KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI
Sbjct: 921  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 980

Query: 781  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 840
            VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE
Sbjct: 981  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 1040

Query: 841  RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 900
            RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF
Sbjct: 1041 RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 1100

Query: 901  GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 960
            GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV
Sbjct: 1101 GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 1160

Query: 961  CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 997
            CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC
Sbjct: 1161 CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 1197

BLAST of Cucsat.G11409 vs. ExPASy TrEMBL
Match: A0A5D3D8Y5 (DNA-directed RNA polymerase subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001250 PE=3 SV=1)

HSP 1 Score: 1986 bits (5145), Expect = 0.0
Identity = 980/997 (98.29%), Postives = 983/997 (98.60%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 60
            MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE
Sbjct: 201  MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 260

Query: 61   VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 120
            VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR
Sbjct: 261  VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 320

Query: 121  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 180
            DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPA+KIEDCLNLYLFA
Sbjct: 321  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPAEKIEDCLNLYLFA 380

Query: 181  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 240
            SLKG KQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR
Sbjct: 381  SLKGLKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 440

Query: 241  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 300
            RMEKALQRDLYGDRQV PIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR
Sbjct: 441  RMEKALQRDLYGDRQVRPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 500

Query: 301  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 360
            ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV
Sbjct: 501  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 560

Query: 361  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 420
            SLNT K I PTLF CGMENL DNTSTS CGKYRIFLDGEWVGVCEDSLSFVT VRRKRRR
Sbjct: 561  SLNTTKPIIPTLFCCGMENLADNTSTSLCGKYRIFLDGEWVGVCEDSLSFVTKVRRKRRR 620

Query: 421  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 480
            NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE
Sbjct: 621  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 680

Query: 481  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 540
            L+GTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ
Sbjct: 681  LVGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 740

Query: 541  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 600
            SQKHSNQAIGFS TNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSH GILPKPE
Sbjct: 741  SQKHSNQAIGFSTTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHTGILPKPE 800

Query: 601  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 660
            FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEH RSYKAEVDNKESSEKRRKSD
Sbjct: 801  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHTRSYKAEVDNKESSEKRRKSD 860

Query: 661  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 720
            D INFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ
Sbjct: 861  DTINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 920

Query: 721  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 780
            KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI
Sbjct: 921  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 980

Query: 781  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 840
            VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE
Sbjct: 981  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 1040

Query: 841  RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 900
            RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF
Sbjct: 1041 RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 1100

Query: 901  GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 960
            GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRK+RGPYCRV
Sbjct: 1101 GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKIRGPYCRV 1160

Query: 961  CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 997
            CES DDIVRVNVPYGAKLLCQELFSMGINLKFETQLC
Sbjct: 1161 CESVDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 1197

BLAST of Cucsat.G11409 vs. ExPASy TrEMBL
Match: A0A1S3BUT1 (DNA-directed RNA polymerase subunit beta OS=Cucumis melo OX=3656 GN=LOC103493748 PE=3 SV=1)

HSP 1 Score: 1986 bits (5145), Expect = 0.0
Identity = 980/997 (98.29%), Postives = 983/997 (98.60%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 60
            MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE
Sbjct: 201  MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 260

Query: 61   VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 120
            VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR
Sbjct: 261  VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 320

Query: 121  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 180
            DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPA+KIEDCLNLYLFA
Sbjct: 321  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPAEKIEDCLNLYLFA 380

Query: 181  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 240
            SLKG KQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR
Sbjct: 381  SLKGLKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 440

Query: 241  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 300
            RMEKALQRDLYGDRQV PIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR
Sbjct: 441  RMEKALQRDLYGDRQVRPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 500

Query: 301  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 360
            ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV
Sbjct: 501  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 560

Query: 361  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 420
            SLNT K I PTLF CGMENL DNTSTS CGKYRIFLDGEWVGVCEDSLSFVT VRRKRRR
Sbjct: 561  SLNTTKPIIPTLFCCGMENLADNTSTSLCGKYRIFLDGEWVGVCEDSLSFVTKVRRKRRR 620

Query: 421  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 480
            NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE
Sbjct: 621  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 680

Query: 481  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 540
            L+GTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ
Sbjct: 681  LVGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 740

Query: 541  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 600
            SQKHSNQAIGFS TNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSH GILPKPE
Sbjct: 741  SQKHSNQAIGFSTTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHTGILPKPE 800

Query: 601  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 660
            FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEH RSYKAEVDNKESSEKRRKSD
Sbjct: 801  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHTRSYKAEVDNKESSEKRRKSD 860

Query: 661  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 720
            D INFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ
Sbjct: 861  DTINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 920

Query: 721  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 780
            KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI
Sbjct: 921  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 980

Query: 781  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 840
            VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE
Sbjct: 981  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 1040

Query: 841  RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 900
            RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF
Sbjct: 1041 RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 1100

Query: 901  GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 960
            GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRK+RGPYCRV
Sbjct: 1101 GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKIRGPYCRV 1160

Query: 961  CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 997
            CES DDIVRVNVPYGAKLLCQELFSMGINLKFETQLC
Sbjct: 1161 CESVDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 1197

BLAST of Cucsat.G11409 vs. ExPASy TrEMBL
Match: A0A6J1FHZ2 (DNA-directed RNA polymerase subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111445938 PE=3 SV=1)

HSP 1 Score: 1925 bits (4986), Expect = 0.0
Identity = 945/997 (94.78%), Postives = 971/997 (97.39%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 60
            MVNSDLCWMKD+QKRDCEFD GGYFLIKGAEKIFIAQEQICLRRLWISN QGWTVAYRSE
Sbjct: 197  MVNSDLCWMKDDQKRDCEFDHGGYFLIKGAEKIFIAQEQICLRRLWISNNQGWTVAYRSE 256

Query: 61   VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 120
            VKRNRLIIRLVENSKSE+LKSKEKVLNVYFLSTE+PVWILFFALGVSSD+EIVDLID+GR
Sbjct: 257  VKRNRLIIRLVENSKSEELKSKEKVLNVYFLSTEIPVWILFFALGVSSDREIVDLIDFGR 316

Query: 121  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 180
            +DPTVLNILFASVR VDNDDKWKDFRRGKRAL FL+NE++KTSFPPA+KIEDCLNLYLFA
Sbjct: 317  EDPTVLNILFASVRVVDNDDKWKDFRRGKRALNFLENEVKKTSFPPAEKIEDCLNLYLFA 376

Query: 181  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 240
            SLKG KQKC FLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELA ELLERE+KVHIAHARR
Sbjct: 377  SLKGLKQKCRFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAGELLEREIKVHIAHARR 436

Query: 241  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 300
            RMEKALQRDLYGDR V PIEHY DASIITNGLSRAFSTGAWAH+FKRMERISGVVATLGR
Sbjct: 437  RMEKALQRDLYGDRDVRPIEHYFDASIITNGLSRAFSTGAWAHSFKRMERISGVVATLGR 496

Query: 301  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 360
            ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLS TGLV
Sbjct: 497  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSITGLV 556

Query: 361  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 420
            SLNT K + PTLFRCGMEN+VD+TSTS  GK+RIFL+GEWVGVCEDSLSFVT VRRKRRR
Sbjct: 557  SLNTLKPVVPTLFRCGMENVVDDTSTSLSGKHRIFLNGEWVGVCEDSLSFVTKVRRKRRR 616

Query: 421  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 480
            N  LHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGE+YTFQSLLDKGIIE
Sbjct: 617  NLLLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGESYTFQSLLDKGIIE 676

Query: 481  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 540
            LIGTEEEEDCR AWSIKHL ED+GT+KY+HCELD SFLLGLSCGLVPFANHDHARRALFQ
Sbjct: 677  LIGTEEEEDCRTAWSIKHLTEDQGTSKYTHCELDTSFLLGLSCGLVPFANHDHARRALFQ 736

Query: 541  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 600
            SQKHSNQAIGFS TNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSH GILPKPE
Sbjct: 737  SQKHSNQAIGFSTTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHTGILPKPE 796

Query: 601  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 660
            FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEH RSYKAEVDNKESSEKRRKSD
Sbjct: 797  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHTRSYKAEVDNKESSEKRRKSD 856

Query: 661  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 720
            DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ
Sbjct: 857  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 916

Query: 721  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 780
            KVVLSSNDDGKN+AVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI
Sbjct: 917  KVVLSSNDDGKNFAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 976

Query: 781  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 840
            VINPHAFPSRQTPGQLLEAALGKGIA+GGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE
Sbjct: 977  VINPHAFPSRQTPGQLLEAALGKGIASGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 1036

Query: 841  RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 900
            RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF
Sbjct: 1037 RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 1096

Query: 901  GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 960
            GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVA+VIQRSVAGGRK+RGPYCRV
Sbjct: 1097 GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVANVIQRSVAGGRKIRGPYCRV 1156

Query: 961  CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 997
            CES DDIVRVNVPYGAKLLCQELFSMGINLKFETQ+C
Sbjct: 1157 CESVDDIVRVNVPYGAKLLCQELFSMGINLKFETQVC 1193

BLAST of Cucsat.G11409 vs. ExPASy TrEMBL
Match: A0A6J1IXN3 (DNA-directed RNA polymerase subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111480863 PE=3 SV=1)

HSP 1 Score: 1921 bits (4976), Expect = 0.0
Identity = 943/997 (94.58%), Postives = 970/997 (97.29%), Query Frame = 0

Query: 1    MVNSDLCWMKDEQKRDCEFDRGGYFLIKGAEKIFIAQEQICLRRLWISNVQGWTVAYRSE 60
            MVNSDLCWMKD+QKRDCEFD GGYFLIKGAEKIFIAQEQICLRRLWISN QGWTVAYRSE
Sbjct: 197  MVNSDLCWMKDDQKRDCEFDHGGYFLIKGAEKIFIAQEQICLRRLWISNNQGWTVAYRSE 256

Query: 61   VKRNRLIIRLVENSKSEDLKSKEKVLNVYFLSTEVPVWILFFALGVSSDKEIVDLIDYGR 120
            VKRNRLIIRLVENSKSE+LKSKEKVLNVYFLSTE+PVWILFFALGVSSD+EIVDLID+GR
Sbjct: 257  VKRNRLIIRLVENSKSEELKSKEKVLNVYFLSTEIPVWILFFALGVSSDREIVDLIDFGR 316

Query: 121  DDPTVLNILFASVREVDNDDKWKDFRRGKRALTFLDNEIRKTSFPPADKIEDCLNLYLFA 180
            +DPTVLNILFASVR VDNDDKWKDFRRGKRAL FL+NE++KTSFPPA+KIEDCLNLYLFA
Sbjct: 317  EDPTVLNILFASVRVVDNDDKWKDFRRGKRALNFLENEVKKTSFPPAEKIEDCLNLYLFA 376

Query: 181  SLKGSKQKCHFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAAELLERELKVHIAHARR 240
            SLKG KQKC FLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELA ELLERE+KVHIAHARR
Sbjct: 377  SLKGLKQKCRFLGYMVKCLLQAYTGRRKCDNRDDFRNKRFELAGELLEREIKVHIAHARR 436

Query: 241  RMEKALQRDLYGDRQVHPIEHYLDASIITNGLSRAFSTGAWAHAFKRMERISGVVATLGR 300
            RMEKALQRDLYGDR V PIEHY DASIITNGLSRAFSTGAWAH+FKRMERISGVVATLGR
Sbjct: 437  RMEKALQRDLYGDRDVRPIEHYFDASIITNGLSRAFSTGAWAHSFKRMERISGVVATLGR 496

Query: 301  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSGTGLV 360
            ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLS TGLV
Sbjct: 497  ANPLQTMAELRRTRQQVAYTGKVGDARYPHPSHWGRVCFLSTPDGENCGLIKNLSITGLV 556

Query: 361  SLNTKKSITPTLFRCGMENLVDNTSTSFCGKYRIFLDGEWVGVCEDSLSFVTNVRRKRRR 420
            SLNT K + PTLFRCGMEN+VD+TSTS  GK++IFL+GEWVGVCEDSLSFVT VR KRRR
Sbjct: 557  SLNTLKPVVPTLFRCGMENVVDDTSTSLSGKHKIFLNGEWVGVCEDSLSFVTKVRLKRRR 616

Query: 421  NPFLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGENYTFQSLLDKGIIE 480
            N  LHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGE+YTFQSLLDKGIIE
Sbjct: 617  NLLLHQVEVKRDEQLKEVRIFSDAGRILRPLLVVENLNRIDKSKGESYTFQSLLDKGIIE 676

Query: 481  LIGTEEEEDCRVAWSIKHLMEDEGTTKYSHCELDMSFLLGLSCGLVPFANHDHARRALFQ 540
            LIGTEEEEDCR AWSIKHL ED+GT+KY+HCELD SFLLGLSCGLVPFANHDHARRALFQ
Sbjct: 677  LIGTEEEEDCRTAWSIKHLTEDQGTSKYTHCELDTSFLLGLSCGLVPFANHDHARRALFQ 736

Query: 541  SQKHSNQAIGFSPTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHAGILPKPE 600
            SQKHSNQAIGFS TNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSH GILPKPE
Sbjct: 737  SQKHSNQAIGFSTTNSNFRVDTLSHQLHYPQRPLFRTMTADCLGTPGYLSSHTGILPKPE 796

Query: 601  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHIRSYKAEVDNKESSEKRRKSD 660
            FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEH RSYKAEVDNKESSEKRRKSD
Sbjct: 797  FYNGQNAIVAVNVHLGYNQEDSLVMNRASLERGMFRSEHTRSYKAEVDNKESSEKRRKSD 856

Query: 661  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 720
            DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ
Sbjct: 857  DAINFGKTQSKIGRVDSLDDDGFPYIGANLQSGDIVIGRCAESGADHSIKLKHTEKGMVQ 916

Query: 721  KVVLSSNDDGKNYAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 780
            KVVLSSNDDGKN+AVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI
Sbjct: 917  KVVLSSNDDGKNFAVVSLRQVRSPCLGDKFSSMHGQKGVLGFLESQENFPFTIQGIVPDI 976

Query: 781  VINPHAFPSRQTPGQLLEAALGKGIAAGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 840
            VINPHAFPSRQTPGQLLEAALGKGIA+GGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE
Sbjct: 977  VINPHAFPSRQTPGQLLEAALGKGIASGGSLKYATPFSTPSVDAITDQLHRAGFSRWGSE 1036

Query: 841  RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 900
            RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF
Sbjct: 1037 RVYNGRTGEMMRSLIFMGPTFYQRLIHMAEDKVKFRNTGPVHPLTRQPVADRKRFGGIKF 1096

Query: 901  GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVASVIQRSVAGGRKMRGPYCRV 960
            GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVA+VIQRSVAGGRK+RGPYCRV
Sbjct: 1097 GEMERDCLIAHGASANLHERLFTLSDSSQMHVCQKCKNVANVIQRSVAGGRKIRGPYCRV 1156

Query: 961  CESFDDIVRVNVPYGAKLLCQELFSMGINLKFETQLC 997
            CES DDIVRVNVPYGAKLLCQELFSMGINLKFETQ+C
Sbjct: 1157 CESVDDIVRVNVPYGAKLLCQELFSMGINLKFETQVC 1193