Cucsat.G10882 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G10882
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Homoserine kinase
Location	ctg1681: 1370619 .. 1372551 (-)
RNA-Seq Expression	Cucsat.G10882
Synteny	Cucsat.G10882

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCTTCATCTTCTTCCTTGACTGCGCTAAAGCGTCTGAACCTGAAAATTATTTCTTCATCTCTCACATAAATTTTCAGGTTTCGATCTCAAAACTCTCTCTCTCACTCTCTCTCTTTTTTCACACTTCCTTCCATGGCTATGCTCTCCTATCAACCGCCATTGAAGTCGTTGACCATTCCTCCAGTTTCTTTATCTAACCCTAAACCTGTTCTCTTCAGGTGCAGTTTGTCTCTTCCATCTAGAACCGCCGTCACTTCCGTCGAACCTCAACCCGTTTTCTCTTCCGTCAAGGCGTTTGCTCCTGCAACCGTCGCTAATTTAGGTCCTGGGTTTGATTTCCTTGGCTGCGCTGTTGATGGCTTGGGAGATTATGTCTCTCTTAGTGTTGATTCCAATGTTCATCCAGGTGAAGTTGCGATTTCTGATATTACAGGGAATAACACGAATAAACTTAGTAAAAATCCTCTCTATAATTGTGCTGGTATTGCTGCTATTGAAGTTATGAAAATGCTAGGGATCCGATCTGTTGGTCTTTCTCTTTCGCTTGAGAAAGGTTTGCCGTTAGGGAGTGGATTGGGATCTAGTGCTGCGAGTGCAGCTGCTGCGGCGATTGCTGTTAATGGATTGTTCGGTGGGAAATTAGGAGTAGAGGAATTGGTTCTCGCGGGGTTGAAATCGGAAGAGAAGGTTTCTGGGTACCATGCGGATAATGTCGCACCGGCTATCATGGGGGGTTTCATTCTGATTCGAAATTACGAACCCTTGGAATTGATTCGTTTGAAATTCCCCGTCGAGAAGGAGCTGTTCTTCGTGTTGGTCAGCCCGGAATTCGAAGCACCGACGAAGAAAATGCGGGCTGCGTTACCTGCTGAAGTTGGGATGCCACACCATGTGTGGAATTCCAGCCAAGCCGGGGCGTTGGTGGCTGCGGTGCTGCAGGGTGACACGATGGGATTGGGGAAAGCATTGTCATCAGACAAAATTGTGGAACCAAGGCGTTCGCCGTTGATTCCAGGTATGGATGGTGTTAAGAAGGCAGCCATTGCTGCTGGGGCATTTGGGTGCACGATAAGCGGAGCAGGGCCAACAGCGGTGGCGGTGATCGATAACGAAGAGAAGGGGAAGGAGATTGGTGAGAGGATGGTTATGGCATTTCTGAAGGAAGGAAATTTGAAAGCTACGGCATCTGTAAAGAGACTAGATCGAGTTGGTGCAAGGCTTATTGGATCAACTCCTTTAGATAGAGTTTTATGAGAAGCATATGTTTATGATACTTTGATGCTCTGCTTCAAATGCTGACTTTCTGTGGCTTCATCATTTGAGATATGGTTAATAAATTTGATTCCGCCATAGAAATGGGTATCTGAATTATCTCATGCTATGTTTTTTGTTGTTTTGATTGCAGAAGCATGGATTGGAATGATCTTGTGCTGATTCTCTCTTCCAACCAAGAGGCATGTTTCTTTTTTTGTTTATCTTTTTAAGGTTTTGATTGATGGTAGTTTTATTCCTATAGATTAGCTTCATATTGGGTTTTGATCCTTATTATTTGCATAAAAATGTAGAATGTTGGTTAAAAAACCCATAAAAGCAAAATTAGCCAACTGAGGAGAATAATTCTAAGCTTAAAAAGCTATTTTGATAGTAGTAGAATAATTGTTAAAATTGATTTCAAAAGCTAGCTGGCTTTACAATGTTCTTCTAAGGGTATATTCGAAAGGCTTTGAGGAAGGCATAATCCATTGTTAGAGTGATATTTTAACTCTTTAAGATTAAACCCCTCAAGCCAGGTTTGGAGTGATTTTGATTGCTTTTGCTGCCAAGTACCAAGTTGCTTTTAGTAGTTCAATGTTATGAAAATAATTTTTGTTTGACATCCATAATGTTGTTTTCATCCAGGGTAATAAATATTGAATTATACCTTTTTATTT

Coding sequence (CDS)

ATGAAAATGCTAGGGATCCGATCTGTTGGTCTTTCTCTTTCGCTTGAGAAAGGTTTGCCGTTAGGGAGTGGATTGGGATCTAGTGCTGCGAGTGCAGCTGCTGCGGCGATTGCTGTTAATGGATTGTTCGGTGGGAAATTAGGAGTAGAGGAATTGGTTCTCGCGGGGTTGAAATCGGAAGAGAAGGTTTCTGGGTACCATGCGGATAATGTCGCACCGGCTATCATGGGGGGTTTCATTCTGATTCGAAATTACGAACCCTTGGAATTGATTCGTTTGAAATTCCCCGTCGAGAAGGAGCTGTTCTTCGTGTTGGTCAGCCCGGAATTCGAAGCACCGACGAAGAAAATGCGGGCTGCGTTACCTGCTGAAGTTGGGATGCCACACCATGTGTGGAATTCCAGCCAAGCCGGGGCGTTGGTGGCTGCGGTGCTGCAGGGTGACACGATGGGATTGGGGAAAGCATTGTCATCAGACAAAATTGTGGAACCAAGGCGTTCGCCGTTGATTCCAGGTATGGATGGTGTTAAGAAGGCAGCCATTGCTGCTGGGGCATTTGGGTGCACGATAAGCGGAGCAGGGCCAACAGCGGTGGCGGTGATCGATAACGAAGAGAAGGGGAAGGAGATTGGTGAGAGGATGGTTATGGCATTTCTGAAGGAAGGAAATTTGAAAGCTACGGCATCTGTAAAGAGACTAGATCGAGTTGGTGCAAGGCTTATTGGATCAACTCCTTTAGATAGAGTTTTATGA

Protein sequence

MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARLIGSTPLDRVL

Homology

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BLAST of Cucsat.G10882 vs. ExPASy Swiss-Prot
Match: Q8L7R2 (Homoserine kinase OS=Arabidopsis thaliana OX=3702 GN=HSK PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 9.4e-101
Identity = 193/243 (79.42%), Postives = 218/243 (89.71%), Query Frame = 0


Query: 1   MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 60
           MKMLGIRSVGLSL L KGLPLGSGLGSSAASAAAAA+AVN +FG KLG ++LVLAGL+SE
Sbjct: 125 MKMLGIRSVGLSLDLHKGLPLGSGLGSSAASAAAAAVAVNEIFGRKLGSDQLVLAGLESE 184

Query: 61  EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 120
            KVSGYHADN+APAIMGGF+LIRNYEPL+L  L+FP +K+LFFVLVSP+FEAPTKKMRAA
Sbjct: 185 AKVSGYHADNIAPAIMGGFVLIRNYEPLDLKPLRFPSDKDLFFVLVSPDFEAPTKKMRAA 244

Query: 121 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 180
           LP E+ M HHVWNSSQA ALVAAVL+GD + LGKALSSDKIVEP R+PLIPGM+ VKKAA
Sbjct: 245 LPTEIPMVHHVWNSSQAAALVAAVLEGDAVMLGKALSSDKIVEPTRAPLIPGMEAVKKAA 304

Query: 181 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 240
           + AGAFGCTISGAGPTAVAVID+EEKG+ IGE+MV AF K G+LK+ ASVK+LD VGARL
Sbjct: 305 LEAGAFGCTISGAGPTAVAVIDSEEKGQVIGEKMVEAFWKVGHLKSVASVKKLDNVGARL 364

Query: 241 IGS 244
           + S
Sbjct: 365 VNS 367

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BLAST of Cucsat.G10882 vs. ExPASy Swiss-Prot
Match: Q8KAW7 (Homoserine kinase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=thrB PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.3e-38
Identity = 104/236 (44.07%), Postives = 140/236 (59.32%), Query Frame = 0


Query: 11  LSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSEEKVSG-YHAD 70
           + L L+K LPL SG+GSSAASAAAA IA N LFG      ELV   ++ E    G  HAD
Sbjct: 91  IDLVLKKNLPLSSGMGSSAASAAAALIAANELFGSPCTKMELVHFAIEGERVACGSAHAD 150

Query: 71  NVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAALPAEVGMPH 130
           N APA++G FILIR+Y PL+LI +K P  K LF  LV P  E  T   R+ LP  + +  
Sbjct: 151 NAAPAMLGNFILIRSYNPLDLITIKPP--KNLFGTLVHPHTELKTSFARSVLPKSIPLST 210

Query: 131 HVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAAIAAGAFGCT 190
                   GAL+A +L  D   +G+AL  D + EP+R+PLIPG + VK+AA+ AGA GC+
Sbjct: 211 ATQQWGNVGALIAGLLMEDYDLIGRAL-VDVVAEPKRAPLIPGFNEVKQAALDAGALGCS 270

Query: 191 ISGAGPTAVAVIDNEEKGKEIGERMVMAFL-KEGNLKATASVKRLDRVGARLIGST 245
           I+G+GP+  A   + +  + +G  M  AFL     L++   V  +   GAR+I +T
Sbjct: 271 IAGSGPSVFAFSSSRQTAEAVGSAMQSAFLHSRAALQSDMWVSPICSQGARIISTT 323

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BLAST of Cucsat.G10882 vs. ExPASy Swiss-Prot
Match: A4SGN0 (Homoserine kinase OS=Chlorobium phaeovibrioides (strain DSM 265 / 1930) OX=290318 GN=thrB PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.5e-37
Identity = 104/233 (44.64%), Postives = 140/233 (60.09%), Query Frame = 0


Query: 11  LSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSEEKVSG-YHAD 70
           +SL L+K LPL SG+GSSAASAAAA  A N L G      ELV   ++ E    G  HAD
Sbjct: 91  ISLELKKHLPLSSGMGSSAASAAAALAAANELLGRPCTKMELVHFAIEGERVACGSAHAD 150

Query: 71  NVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAALPAEVGMPH 130
           N APAI+G F+LIR+Y PL+LI +  P  ++LF  LV P  E  T   R+ LP ++ +  
Sbjct: 151 NAAPAILGNFVLIRSYTPLDLIVI--PPPEKLFCSLVHPHTELRTSFARSVLPTDITLKD 210

Query: 131 HVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAAIAAGAFGCT 190
                   GALVA +L  D   +G++L  D + EP+R+PLIPG   VK+AA+ AGA GC+
Sbjct: 211 ATRQWGNVGALVAGLLTSDYALVGRSL-VDVVAEPKRAPLIPGFAEVKEAALNAGALGCS 270

Query: 191 ISGAGPTAVAVIDNEEKGKEIGERMVMAFL-KEGNLKATASVKRLDRVGARLI 242
           I+G+GP+  A  D+ E     GE M  AFL  + +LK+   V  + R GAR++
Sbjct: 271 IAGSGPSIFAFSDSAETAHAAGEAMQKAFLASKKSLKSDMWVSPICREGARIL 320

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BLAST of Cucsat.G10882 vs. ExPASy Swiss-Prot
Match: A1BJF6 (Homoserine kinase OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=thrB PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 4.3e-37
Identity = 101/233 (43.35%), Postives = 138/233 (59.23%), Query Frame = 0


Query: 11  LSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSEEKVSG-YHAD 70
           + L L+K LPL SG+GSSAASAAAA  A N L G      ELV   ++ E    G  HAD
Sbjct: 91  IDLELKKNLPLSSGMGSSAASAAAALAAANELLGQPCSKMELVHFAVEGERVACGSAHAD 150

Query: 71  NVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAALPAEVGMPH 130
           N APAI+G F+LIR+Y PL+LI +  P  + LF  LV P  E  T   R+ LP  + +  
Sbjct: 151 NAAPAILGNFVLIRSYAPLDLITI--PSPEHLFCTLVHPHTELKTSFARSVLPRSIPLKT 210

Query: 131 HVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAAIAAGAFGCT 190
                   GAL+A +L+ D   +G+AL  D + EP+R+PLIPG   VK AAI  GA GC+
Sbjct: 211 ATQQWGNVGALIAGLLKSDYDLIGRAL-VDVVAEPKRAPLIPGFLDVKHAAIDCGALGCS 270

Query: 191 ISGAGPTAVAVIDNEEKGKEIGERMVMAF-LKEGNLKATASVKRLDRVGARLI 242
           I+G+GP+  A   ++E  + +G+ M  AF L E NLK+   V  + + GA+++
Sbjct: 271 IAGSGPSVFAFSSSKETAERVGQAMREAFLLPETNLKSDMWVSPICKEGAKVL 320

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BLAST of Cucsat.G10882 vs. ExPASy Swiss-Prot
Match: B3QWX6 (Homoserine kinase OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) OX=517418 GN=thrB PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 4.8e-36
Identity = 103/239 (43.10%), Postives = 139/239 (58.16%), Query Frame = 0


Query: 5   GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSEEKV 64
           GI   G  S+ L+K LPL SG+GSSA+SAAAA IA N L G      ELV   ++ E   
Sbjct: 84  GIEQSGTFSIELKKNLPLSSGMGSSASSAAAALIAANSLLGSPCTKMELVPFVMEGERIA 143

Query: 65  SG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAALP 124
            G  HADN APA++G FIL R+Y+PL+LI +  P   EL+  LV P  E PT   R+ L 
Sbjct: 144 CGSIHADNAAPAMLGNFILTRSYDPLDLIPISTP--PELYCSLVHPHIEVPTSHARSILK 203

Query: 125 AEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAAIA 184
            E+ +   V      GALVA +L+ D   +G+AL  D + EP R+PLIPG   VK AA+ 
Sbjct: 204 KEIALSSAVRQWGNVGALVAGLLRCDYELIGRAL-EDVVAEPVRAPLIPGFYDVKHAALD 263

Query: 185 AGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARLI 242
           AGA G +I+G+GP+  A  D+  K + I + M   F K  NL++      + R G+R++
Sbjct: 264 AGALGGSIAGSGPSIFAFSDSLAKAETIAKAMQETFRKVSNLESDIWFCPVSREGSRIL 319

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BLAST of Cucsat.G10882 vs. NCBI nr
Match: XP_004153462.1 (homoserine kinase [Cucumis sativus] >KGN43352.1 hypothetical protein Csa_020446 [Cucumis sativus])

HSP 1 Score: 467 bits (1201), Expect = 2.10e-163
Identity = 250/250 (100.00%), Postives = 250/250 (100.00%), Query Frame = 0


Query: 1   MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 60
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 61  EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 120
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243

Query: 121 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 180
           LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 181 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 240
           IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 363

Query: 241 IGSTPLDRVL 250
           IGSTPLDRVL
Sbjct: 364 IGSTPLDRVL 373

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BLAST of Cucsat.G10882 vs. NCBI nr
Match: KAA0059609.1 (homoserine kinase [Cucumis melo var. makuwa] >TYK08142.1 homoserine kinase [Cucumis melo var. makuwa])

HSP 1 Score: 464 bits (1193), Expect = 3.46e-162
Identity = 248/250 (99.20%), Postives = 249/250 (99.60%), Query Frame = 0


Query: 1   MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 60
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 61  EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 120
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKMRAA 243

Query: 121 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 180
           LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 181 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 240
           IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKAAASVKRLDRVGARL 363

Query: 241 IGSTPLDRVL 250
           IGSTPLDRVL
Sbjct: 364 IGSTPLDRVL 373

[Close]

BLAST of Cucsat.G10882 vs. NCBI nr
Match: XP_008451216.1 (PREDICTED: homoserine kinase [Cucumis melo])

HSP 1 Score: 462 bits (1189), Expect = 1.40e-161
Identity = 247/250 (98.80%), Postives = 249/250 (99.60%), Query Frame = 0


Query: 1   MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 60
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 61  EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 120
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKMRAA 243

Query: 121 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 180
           LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 181 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 240
           IAAGAFGCTISGAGPTAVAVIDNEE+GKEIGERMVMAFLKEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEQGKEIGERMVMAFLKEGNLKAAASVKRLDRVGARL 363

Query: 241 IGSTPLDRVL 250
           IGSTPLDRVL
Sbjct: 364 IGSTPLDRVL 373

[Close]

BLAST of Cucsat.G10882 vs. NCBI nr
Match: XP_038888998.1 (homoserine kinase [Benincasa hispida])

HSP 1 Score: 461 bits (1185), Expect = 5.70e-161
Identity = 246/250 (98.40%), Postives = 248/250 (99.20%), Query Frame = 0


Query: 1   MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 60
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 61  EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 120
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243

Query: 121 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 180
           LP+EVGMPHHVWNSSQAGALVAAVLQGD MGLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPSEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 181 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 240
           IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAF+KEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVGARL 363

Query: 241 IGSTPLDRVL 250
           IGSTPLDRVL
Sbjct: 364 IGSTPLDRVL 373

[Close]

BLAST of Cucsat.G10882 vs. NCBI nr
Match: XP_022135832.1 (homoserine kinase [Momordica charantia])

HSP 1 Score: 443 bits (1140), Expect = 3.36e-154
Identity = 236/245 (96.33%), Postives = 241/245 (98.37%), Query Frame = 0


Query: 1   MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 60
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182

Query: 61  EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 120
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242

Query: 121 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 180
           LPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEPRRSP+IPGMDGVKKAA
Sbjct: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPMIPGMDGVKKAA 302

Query: 181 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 240
           IAAGAFGCTISGAGPTAVAVID+EE+GKEIGERMVMAFLKEGNLKA A VKRLDRVGARL
Sbjct: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVALVKRLDRVGARL 362

Query: 241 IGSTP 245
           +GSTP
Sbjct: 363 VGSTP 367

[Close]

BLAST of Cucsat.G10882 vs. ExPASy TrEMBL
Match: A0A0A0K4Y4 (Homoserine kinase OS=Cucumis sativus OX=3659 GN=Csa_7G025730 PE=3 SV=1)

HSP 1 Score: 467 bits (1201), Expect = 1.01e-163
Identity = 250/250 (100.00%), Postives = 250/250 (100.00%), Query Frame = 0


Query: 1   MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 60
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 61  EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 120
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243

Query: 121 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 180
           LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 181 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 240
           IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 363

Query: 241 IGSTPLDRVL 250
           IGSTPLDRVL
Sbjct: 364 IGSTPLDRVL 373

[Close]

BLAST of Cucsat.G10882 vs. ExPASy TrEMBL
Match: A0A5A7V1E5 (Homoserine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00200 PE=3 SV=1)

HSP 1 Score: 464 bits (1193), Expect = 1.67e-162
Identity = 248/250 (99.20%), Postives = 249/250 (99.60%), Query Frame = 0


Query: 1   MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 60
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 61  EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 120
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKMRAA 243

Query: 121 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 180
           LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 181 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 240
           IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKAAASVKRLDRVGARL 363

Query: 241 IGSTPLDRVL 250
           IGSTPLDRVL
Sbjct: 364 IGSTPLDRVL 373

[Close]

BLAST of Cucsat.G10882 vs. ExPASy TrEMBL
Match: A0A1S3BR18 (Homoserine kinase OS=Cucumis melo OX=3656 GN=LOC103492575 PE=3 SV=1)

HSP 1 Score: 462 bits (1189), Expect = 6.80e-162
Identity = 247/250 (98.80%), Postives = 249/250 (99.60%), Query Frame = 0


Query: 1   MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 60
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183

Query: 61  EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 120
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKMRAA 243

Query: 121 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 180
           LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA
Sbjct: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303

Query: 181 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 240
           IAAGAFGCTISGAGPTAVAVIDNEE+GKEIGERMVMAFLKEGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVIDNEEQGKEIGERMVMAFLKEGNLKAAASVKRLDRVGARL 363

Query: 241 IGSTPLDRVL 250
           IGSTPLDRVL
Sbjct: 364 IGSTPLDRVL 373

[Close]

BLAST of Cucsat.G10882 vs. ExPASy TrEMBL
Match: A0A6J1C3W0 (Homoserine kinase OS=Momordica charantia OX=3673 GN=LOC111007689 PE=3 SV=1)

HSP 1 Score: 443 bits (1140), Expect = 1.63e-154
Identity = 236/245 (96.33%), Postives = 241/245 (98.37%), Query Frame = 0


Query: 1   MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 60
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182

Query: 61  EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 120
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242

Query: 121 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 180
           LPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEPRRSP+IPGMDGVKKAA
Sbjct: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPMIPGMDGVKKAA 302

Query: 181 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 240
           IAAGAFGCTISGAGPTAVAVID+EE+GKEIGERMVMAFLKEGNLKA A VKRLDRVGARL
Sbjct: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVALVKRLDRVGARL 362

Query: 241 IGSTP 245
           +GSTP
Sbjct: 363 VGSTP 367

[Close]

BLAST of Cucsat.G10882 vs. ExPASy TrEMBL
Match: A0A6J1GJP3 (Homoserine kinase OS=Cucurbita moschata OX=3662 GN=LOC111454929 PE=3 SV=1)

HSP 1 Score: 440 bits (1131), Expect = 4.38e-153
Identity = 234/249 (93.98%), Postives = 241/249 (96.79%), Query Frame = 0


Query: 1   MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 60
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVE+LVLAGLKSE
Sbjct: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEDLVLAGLKSE 183

Query: 61  EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 120
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243

Query: 121 LPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 180
           LPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEP R PLIPGM GVKKAA
Sbjct: 244 LPAEVGMPHHVWNCSQAGALVAAVLQGDVIGLGKALSSDKIVEPTRVPLIPGMGGVKKAA 303

Query: 181 IAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVGARL 240
           IAAGAFGCTISGAGPTAVAV DN+E+GKEIGE MVMAF++EGNLKA ASVKRLDRVGARL
Sbjct: 304 IAAGAFGCTISGAGPTAVAVTDNKERGKEIGESMVMAFMREGNLKAVASVKRLDRVGARL 363

Query: 241 IGSTPLDRV 249
           IGSTP+DRV
Sbjct: 364 IGSTPIDRV 372

The following BLAST results are available for this feature:

BLAST of Cucsat.G10882 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L7R2	9.4e-101	79.42	Homoserine kinase OS=Arabidopsis thaliana OX=3702 GN=HSK PE=1 SV=1	[more]
Q8KAW7	2.3e-38	44.07	Homoserine kinase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC...	[more]
A4SGN0	2.5e-37	44.64	Homoserine kinase OS=Chlorobium phaeovibrioides (strain DSM 265 / 1930) OX=29031...	[more]
A1BJF6	4.3e-37	43.35	Homoserine kinase OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=t...	[more]
B3QWX6	4.8e-36	43.10	Homoserine kinase OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) OX=51...	[more]

BLAST of Cucsat.G10882 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004153462.1	2.10e-163	100.00	homoserine kinase [Cucumis sativus] >KGN43352.1 hypothetical protein Csa_020446 ...	[more]
KAA0059609.1	3.46e-162	99.20	homoserine kinase [Cucumis melo var. makuwa] >TYK08142.1 homoserine kinase [Cucu...	[more]
XP_008451216.1	1.40e-161	98.80	PREDICTED: homoserine kinase [Cucumis melo]	[more]
XP_038888998.1	5.70e-161	98.40	homoserine kinase [Benincasa hispida]	[more]
XP_022135832.1	3.36e-154	96.33	homoserine kinase [Momordica charantia]	[more]

BLAST of Cucsat.G10882 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K4Y4	1.01e-163	100.00	Homoserine kinase OS=Cucumis sativus OX=3659 GN=Csa_7G025730 PE=3 SV=1	[more]
A0A5A7V1E5	1.67e-162	99.20	Homoserine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00...	[more]
A0A1S3BR18	6.80e-162	98.80	Homoserine kinase OS=Cucumis melo OX=3656 GN=LOC103492575 PE=3 SV=1	[more]
A0A6J1C3W0	1.63e-154	96.33	Homoserine kinase OS=Momordica charantia OX=3673 GN=LOC111007689 PE=3 SV=1	[more]
A0A6J1GJP3	4.38e-153	93.98	Homoserine kinase OS=Cucurbita moschata OX=3662 GN=LOC111454929 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00958	HOMSERKINASE	coord: 185..200 score: 47.92 coord: 26..41 score: 45.83 coord: 105..122 score: 38.89 coord: 65..78 score: 52.78
None	No IPR available	PANTHER	PTHR20861	HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE	coord: 1..243
None	No IPR available	PANTHER	PTHR20861:SF8	BNAA09G09000D PROTEIN	coord: 1..243
IPR006204	GHMP kinase N-terminal domain	PFAM	PF00288	GHMP_kinases_N	coord: 13..78 e-value: 1.8E-10 score: 40.9
IPR036554	GHMP kinase, C-terminal domain superfamily	GENE3D	3.30.70.890		coord: 99..230 e-value: 4.6E-37 score: 128.8
IPR036554	GHMP kinase, C-terminal domain superfamily	SUPERFAMILY	55060	GHMP Kinase, C-terminal domain	coord: 100..214
IPR000870	Homoserine kinase	TIGRFAM	TIGR00191	TIGR00191	coord: 3..240 e-value: 2.2E-51 score: 172.5
IPR013750	GHMP kinase, C-terminal domain	PFAM	PF08544	GHMP_kinases_C	coord: 141..214 e-value: 1.5E-9 score: 38.1
IPR014721	Ribosomal protein S5 domain 2-type fold, subgroup	GENE3D	3.30.230.10		coord: 1..98 e-value: 3.4E-24 score: 87.2
IPR006203	GHMP kinase, ATP-binding, conserved site	PROSITE	PS00627	GHMP_KINASES_ATP	coord: 19..30
IPR020568	Ribosomal protein S5 domain 2-type fold	SUPERFAMILY	54211	Ribosomal protein S5 domain 2-like	coord: 3..88

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G10882.T1	Cucsat.G10882.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009088	threonine biosynthetic process
biological_process	GO:0006566	threonine metabolic process
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004413	homoserine kinase activity