Cucsat.G10451 (gene) Cucumber (B10) v3

Overview
NameCucsat.G10451
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionRING-type E3 ubiquitin transferase
Locationctg1678: 414224 .. 416153 (-)
RNA-Seq ExpressionCucsat.G10451
SyntenyCucsat.G10451
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GACCTCTTCTAATGTATTTTTTTTCTTTCCCCTACTTTTGTGGTATTCTCTCAAAATTCATCCAAAAGTCTTCTGACCCTTCTTCAATGGCAATGTTAGAAAATCATAAATTTACGTATTAATTTGGTCGTCTTAATGCCAAATGAAATGATCTCCCTCAGCAGACGGCTCACCTAAGCCTTAAACTTATTTATCTTTTCATTTAATCCTTCTTACCTATTAAACATTCAATTTTCTCACCTCAATTCTTAGTCTTTATAAGTATTCCCATTCATTGATCTTTACAATTTCATGTCCAAGTTCATCGTCTATGGAGTTTTTCACATACCCCACTTCTAAATTTCTAACCAAATCATTCTAGTTTCCATTACAATCGAAATTGGGTTGATTCTCTCATCTGGGTCTTCTTCAAAACCTTCGAAACCCTTGAATTCGAGGCGGTTTCTTTACCATTTTGCAGAATTTTGAAATGGGTCCGTCGGAGGAAGATATGGTGGTGAATCAACTGCCGGAGTCTGTGAGGGAGATTTCAGGACTTCCTGAGTGTAACGGTATCTGTAAGAAGATGTATGGGGACCTGATTCGAAGAGTCAAACTATTGAGCCCTCTGTTTGAAGAATTAAGAGATGGGGAAGAAGAAGTTGAGCTGGATGTTCTTAAGGGCTTGGAGTTGCTTAAAATTGCTTTGGATTCTGCTATTGAGCTTCTTAAGTCGGTCAGCCAAGGTAGCAAGCTATTTCAGGTATTGGTTCGTTTTTCTTTCTTCTATGCTCTTCTTAGTTTCTCTCTTTGGTTTATTGAGAATTCATCATGTTAACAAGAAATTTCTTGGCTGATCCATCTGTTTTTTCTTCTGTGTATATGTGAAATGTTTTAACGTTTCGGAATCTTTGATATTCGTCTGTATTTGTTCCATTGTTTGAAGAAAGCCATGTTAATTTGAAGATCTTTTGTAGTTCCTATGATTTTTTCATTTGATCCGATATTCACTGGGAGGTGAATACTTTCTAGACTGCTATCTATTTCGACAAGTGTAATATCATCAGCTTTCAAGAGAATTCATCAGATGCTTTATGGAATTTTCCTTCTCCCTCTTTTGTTTCAGGCTTCGCAGTCGGAAAAGATTGGACTTGAGTTTCATCATATGACAGAAGATATTGAAGCAGCACTCAGCAAGCTTCCCATCGACAAGCTTGGGATCTCGGACGAGGTTCGAGAACAGGTACAGTTATTACCTGACCTCCATGAACTTTCATTGTAAATAGGATTTGTCATGTTCTCTTTGTTTGGTGTTTTTCCATGAGAACCATTTTAGTACTGAATTTTGATTAGTTGAGTTGGATGAGATATATGCTTACCAGTTGATGTCCCAACGGCTAACCGTTGATTCCGTTGGTTTCAGACTGAACTTGTTCATGCGCAATTCAAACGAGCAAAAGAGCGAGTAAATTTAGCCGATACACAATTAGACAAGGATTTAGCTATATTACAAGAAGAAAAAGATCCTGACCCTGCAGTATTGAAGAGACTTTCTGAAAAGTTGCATCTTCGAACTATAAACGAGCTGAAGAAAGAGTCACTAGCGATCCACGAGTTGGTGATCTCGAGTGATGGAGACCCTGAGGATGTTTTTGGTAAAATGTCGTCCATTTTAAAGAAGCTGAAGGATTTTGTACAATCAGAAAACCCGGAAGTTGAAACTTCTCAAGACGAAAAAACTACAACCATTAAACACAGATCTCCTGTCATCCCCGACGATTTCAGATGCCCCATATCTCTTGAATTGATGAGGGATCCAGTCATCGTTTCCACCGGGCAGGTGAACATCAAAATCGACGGATGCAATGCAAGTTCTTTCTTATTTTTCTTATTGTTATGATGGTTATTAATAGATTGTTTTCTGACCTTTTGTAGACATATGAGAGATCTTGCATTCAGAAATGGTTGGATGCAGGGCACAAGACTTGTCCAAAATCTCAGCAAGCTTTACTACATACAGCCTTAACACCAAATTACGTTTTGAAGAGTCTAATTGCTTTGTGGTGCGAGAACAATGGTGTTGAACTGCCAAAGAAGCAAGGTAGCTGTAGAAACAAGAAAGCCGGAAACAATGTTTCAGACTGTGATCGGTCAGCCATCGATGCGTTACTAGTAAAATTGGTAAATGGATCGCCGGAACAAAAAAGATCAGCTGCAGGTGAACTCCGGTTACTAGCAAAGAGGAATTCAGATAACAGAATTTGTATTGCTGAGGCAGGAGCTATTCCGTTCCTTGTTGAACTATTATCGTCAAATGACACTAGGACCCAGGAGCATGCAGTTACAGCATTATTGAACCTTTCAATCAACGACGGTAACAAAAGAACCATTGTCGATTTACGAGCCATTCCCGCTGTCGTAGAAGTGTTGAAAAATGGCAGTATGGAGGCGAGGGAAAATGCAGCTGCAACCCTTTTCAGTTTGTCCGTAATTGACGAGAACAAGGTTGCAATTGGAGCAGCTGGGGCAATTCCTGCTCTTATTACTTTGCTTCGAGAAGGTACACCGAGAGGAAAGAAAGATGCTGCCACTGCTATTTTCAATCTATCAATCTATCAGGGAAACAAAGCTAGAGCAATAAGAGCAGGAATTGTGAATCCACTTATGGGATTTCTAAAAGATGCTGGAGGTGGAATGGTGGATGAAGCTCTAGCCATTCTAGCAATTCTTGCAACTCACCATGAAGGGAAAACAGCAATAGGGGAAGCTGAGCCGATGGCGATTCTGTTGGAGTTCATCAGAACAGGCTCTCCACGAAACCGGGAGAACGCTGCTGCAGTGCTGTGGTCGCTTTGTAGTACGGATTTCGAGCAGTTGAAGTTAGCTAGAGAACATGGAGCAGAAGAGGCATTAAAGGAAGTATCTGAGAATGGCACAGAGAGAGCAAAGAGAAAAGCAGGAAGCATTTTAGAACTGTTTCAACGTTTTGATAAACCATCACCTAATCTATAAACAATTTGGAGGTTTGATTTTGTTATAATTGAGGGGTGATGGAAATGAAGTGAATGCCATCTTCACCCTTGTAAATTACAAGGCATCAAAAGTGAAGGATGCCATTATTTTTATTTATTTTTAAATATATATTACAATATATTGATTATTGTGTTTAAGATTTGAAGGAATTCTATTTTTGTAGTTTGAATGTTTGGTGAAGAAAGTGGAAAAAGGTCCTCCAAGATAAGATTTAATATACCCATTGTAAGCAGATTTCTTTTAGACGATAA

Coding sequence (CDS)

ATGGGTCCGTCGGAGGAAGATATGGTGGTGAATCAACTGCCGGAGTCTGTGAGGGAGATTTCAGGACTTCCTGAGTGTAACGGTATCTGTAAGAAGATGTATGGGGACCTGATTCGAAGAGTCAAACTATTGAGCCCTCTGTTTGAAGAATTAAGAGATGGGGAAGAAGAAGTTGAGCTGGATGTTCTTAAGGGCTTGGAGTTGCTTAAAATTGCTTTGGATTCTGCTATTGAGCTTCTTAAGTCGGTCAGCCAAGGTAGCAAGCTATTTCAGGCTTCGCAGTCGGAAAAGATTGGACTTGAGTTTCATCATATGACAGAAGATATTGAAGCAGCACTCAGCAAGCTTCCCATCGACAAGCTTGGGATCTCGGACGAGGTTCGAGAACAGACTGAACTTGTTCATGCGCAATTCAAACGAGCAAAAGAGCGAGTAAATTTAGCCGATACACAATTAGACAAGGATTTAGCTATATTACAAGAAGAAAAAGATCCTGACCCTGCAGTATTGAAGAGACTTTCTGAAAAGTTGCATCTTCGAACTATAAACGAGCTGAAGAAAGAGTCACTAGCGATCCACGAGTTGGTGATCTCGAGTGATGGAGACCCTGAGGATGTTTTTGGTAAAATGTCGTCCATTTTAAAGAAGCTGAAGGATTTTGTACAATCAGAAAACCCGGAAGTTGAAACTTCTCAAGACGAAAAAACTACAACCATTAAACACAGATCTCCTGTCATCCCCGACGATTTCAGATGCCCCATATCTCTTGAATTGATGAGGGATCCAGTCATCGTTTCCACCGGGCAGACATATGAGAGATCTTGCATTCAGAAATGGTTGGATGCAGGGCACAAGACTTGTCCAAAATCTCAGCAAGCTTTACTACATACAGCCTTAACACCAAATTACGTTTTGAAGAGTCTAATTGCTTTGTGGTGCGAGAACAATGGTGTTGAACTGCCAAAGAAGCAAGGTAGCTGTAGAAACAAGAAAGCCGGAAACAATGTTTCAGACTGTGATCGGTCAGCCATCGATGCGTTACTAGTAAAATTGGTAAATGGATCGCCGGAACAAAAAAGATCAGCTGCAGGTGAACTCCGGTTACTAGCAAAGAGGAATTCAGATAACAGAATTTGTATTGCTGAGGCAGGAGCTATTCCGTTCCTTGTTGAACTATTATCGTCAAATGACACTAGGACCCAGGAGCATGCAGTTACAGCATTATTGAACCTTTCAATCAACGACGGTAACAAAAGAACCATTGTCGATTTACGAGCCATTCCCGCTGTCGTAGAAGTGTTGAAAAATGGCAGTATGGAGGCGAGGGAAAATGCAGCTGCAACCCTTTTCAGTTTGTCCGTAATTGACGAGAACAAGGTTGCAATTGGAGCAGCTGGGGCAATTCCTGCTCTTATTACTTTGCTTCGAGAAGGTACACCGAGAGGAAAGAAAGATGCTGCCACTGCTATTTTCAATCTATCAATCTATCAGGGAAACAAAGCTAGAGCAATAAGAGCAGGAATTGTGAATCCACTTATGGGATTTCTAAAAGATGCTGGAGGTGGAATGGTGGATGAAGCTCTAGCCATTCTAGCAATTCTTGCAACTCACCATGAAGGGAAAACAGCAATAGGGGAAGCTGAGCCGATGGCGATTCTGTTGGAGTTCATCAGAACAGGCTCTCCACGAAACCGGGAGAACGCTGCTGCAGTGCTGTGGTCGCTTTGTAGTACGGATTTCGAGCAGTTGAAGTTAGCTAGAGAACATGGAGCAGAAGAGGCATTAAAGGAAGTATCTGAGAATGGCACAGAGAGAGCAAAGAGAAAAGCAGGAAGCATTTTAGAACTGTTTCAACGTTTTGATAAACCATCACCTAATCTATAA

Protein sequence

MGPSEEDMVVNQLPESVREISGLPECNGICKKMYGDLIRRVKLLSPLFEELRDGEEEVELDVLKGLELLKIALDSAIELLKSVSQGSKLFQASQSEKIGLEFHHMTEDIEAALSKLPIDKLGISDEVREQTELVHAQFKRAKERVNLADTQLDKDLAILQEEKDPDPAVLKRLSEKLHLRTINELKKESLAIHELVISSDGDPEDVFGKMSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQTYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRNKKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFLVELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIVNPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAAAVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL
Homology
BLAST of Cucsat.G10451 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 2.4e-165
Identity = 305/411 (74.21%), Postives = 350/411 (85.16%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           MSS+LK L DFV  E+ + + S   +  + +HRSPVIP+ FRCPISLELM+DPVIVSTGQ
Sbjct: 213 MSSLLKNLVDFVTMESSDPDPSTGSRIVS-RHRSPVIPEYFRCPISLELMKDPVIVSTGQ 272

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERS IQKWLDAGHKTCPKSQ+ LLH  LTPNYVLKSLIALWCE+NG+ELP+ QGSCR 
Sbjct: 273 TYERSSIQKWLDAGHKTCPKSQETLLHAGLTPNYVLKSLIALWCESNGIELPQNQGSCRT 332

Query: 121 KK-AGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPF 180
            K  G++ SDCDR+ + +LL KL NG+ EQ+R+AAGELRLLAKRN DNR+CIAEAGAIP 
Sbjct: 333 TKIGGSSSSDCDRTFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEAGAIPL 392

Query: 181 LVELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAAT 240
           LVELLSS D RTQEH+VTALLNLSIN+GNK  IVD  AI  +VEVLKNGSMEARENAAAT
Sbjct: 393 LVELLSSPDPRTQEHSVTALLNLSINEGNKGAIVDAGAITDIVEVLKNGSMEARENAAAT 452

Query: 241 LFSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGI 300
           LFSLSVIDENKVAIGAAGAI ALI+LL EGT RGKKDAATAIFNL IYQGNK+RA++ GI
Sbjct: 453 LFSLSVIDENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGI 512

Query: 301 VNPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENA 360
           V+PL   LKDAGGGMVDEALAILAIL+T+ EGKTAI EAE + +L+E IRTGSPRNRENA
Sbjct: 513 VDPLTRLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEIIRTGSPRNRENA 572

Query: 361 AAVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQR 411
           AA+LW LC  + E+L +ARE GA+ ALKE++ENGT+RAKRKA S+LEL Q+
Sbjct: 573 AAILWYLCIGNIERLNVAREVGADVALKELTENGTDRAKRKAASLLELIQQ 622

BLAST of Cucsat.G10451 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.5e-151
Identity = 279/415 (67.23%), Postives = 340/415 (81.93%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           MSS+LKKLKD V +E+     +   ++ +IKHRSP+IPD+FRCPISLELM+DPVIVS+GQ
Sbjct: 193 MSSLLKKLKDCVVTED-HANDALTTRSASIKHRSPIIPDEFRCPISLELMQDPVIVSSGQ 252

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERSCIQKWLD+GHKTCPK+QQ L HT+LTPN+VLKSLI+ WCE NG+ELPK + + R+
Sbjct: 253 TYERSCIQKWLDSGHKTCPKTQQPLSHTSLTPNFVLKSLISQWCEANGIELPKNKQNSRD 312

Query: 121 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 180
           KKA  + SD D + + +L+ +L +G+ +++R+AAGE+RLLAKRN +NRICIAEAGAIP L
Sbjct: 313 KKAAKS-SDYDHAGLVSLMNRLRSGNQDEQRAAAGEIRLLAKRNVNNRICIAEAGAIPLL 372

Query: 181 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 240
           V LLSS+D RTQEHAVTALLNLSI++ NK +IVD  AIP +VEVLK GSME RENAAATL
Sbjct: 373 VNLLSSSDPRTQEHAVTALLNLSIHENNKASIVDSHAIPKIVEVLKTGSMETRENAAATL 432

Query: 241 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 300
           FSLSV+DENKV IGAAGAIP LI LL +G+PRGKKDAATAIFNL IYQGNK RA++AGIV
Sbjct: 433 FSLSVVDENKVTIGAAGAIPPLINLLCDGSPRGKKDAATAIFNLCIYQGNKVRAVKAGIV 492

Query: 301 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 360
             LM FL D  GGM+DEAL++L+ILA + EGK  I  +EP+  L+E I+TGSPRNRENAA
Sbjct: 493 IHLMNFLVDPTGGMIDEALSLLSILAGNPEGKIVIARSEPIPPLVEVIKTGSPRNRENAA 552

Query: 361 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPS 416
           A+LW LCS D EQ   A+  G E+ALKE+SE GT+RAKRKA SILEL  + ++ S
Sbjct: 553 AILWLLCSADTEQTLAAKAAGVEDALKELSETGTDRAKRKASSILELMHQANEDS 605

BLAST of Cucsat.G10451 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.5e-135
Identity = 257/418 (61.48%), Postives = 312/418 (74.64%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVE--------TSQDEKTTTIKHRSPVIPDDFRCPISLELMRD 60
           M+ +LK +KDFVQ+E+   E         S  + +T    + PVIPDDFRCPISLE+MRD
Sbjct: 212 MAMVLKMIKDFVQTEDDNGEEQKVGVNSRSNGQTSTAASQKIPVIPDDFRCPISLEMMRD 271

Query: 61  PVIVSTGQTYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELP 120
           PVIVS+GQTYER+CI+KW++ GH TCPK+QQAL  T LTPNYVL+SLIA WCE N +E P
Sbjct: 272 PVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALTSTTLTPNYVLRSLIAQWCEANDIEPP 331

Query: 121 KKQGSCRNKKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIA 180
           K   S R +K  +  S  + + I+ L+ +L  G+PE +RSAAGE+RLLAKRN+DNR+ IA
Sbjct: 332 KPPSSLRPRKVSSFSSPAEANKIEDLMWRLAYGNPEDQRSAAGEIRLLAKRNADNRVAIA 391

Query: 181 EAGAIPFLVELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEA 240
           EAGAIP LV LLS+ D+R QEH+VTALLNLSI + NK  IV   AIP +V+VLK GSMEA
Sbjct: 392 EAGAIPLLVGLLSTPDSRIQEHSVTALLNLSICENNKGAIVSAGAIPGIVQVLKKGSMEA 451

Query: 241 RENAAATLFSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKA 300
           RENAAATLFSLSVIDENKV IGA GAIP L+ LL EGT RGKKDAATA+FNL IYQGNK 
Sbjct: 452 RENAAATLFSLSVIDENKVTIGALGAIPPLVVLLNEGTQRGKKDAATALFNLCIYQGNKG 511

Query: 301 RAIRAGIVNPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGS 360
           +AIRAG++  L   L + G GMVDEALAILAIL++H EGK  IG ++ +  L+EFIRTGS
Sbjct: 512 KAIRAGVIPTLTRLLTEPGSGMVDEALAILAILSSHPEGKAIIGSSDAVPSLVEFIRTGS 571

Query: 361 PRNRENAAAVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQR 411
           PRNRENAAAVL  LCS D + L  A++ G    L +++ NGT+R KRKA  +LE   R
Sbjct: 572 PRNRENAAAVLVHLCSGDPQHLVEAQKLGLMGPLIDLAGNGTDRGKRKAAQLLERISR 629

BLAST of Cucsat.G10451 vs. ExPASy Swiss-Prot
Match: A2ZLU6 (Protein spotted leaf 11 OS=Oryza sativa subsp. indica OX=39946 GN=SPL11 PE=1 SV=2)

HSP 1 Score: 473.0 bits (1216), Expect = 3.5e-132
Identity = 253/415 (60.96%), Postives = 317/415 (76.39%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKT--TTIKHRSPVIPDDFRCPISLELMRDPVIVST 60
           MS +LKK+KDFVQ++NP++      +   +    R   IPD+FRCPISLELM+DPVIVST
Sbjct: 235 MSMLLKKIKDFVQTQNPDMGPPMASRVLDSNGDSRPITIPDEFRCPISLELMKDPVIVST 294

Query: 61  GQTYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSC 120
           GQTYER+CI+KW+ +GH TCP +QQ +  +ALTPNYVL+SLI+ WCE NG+E PK+  S 
Sbjct: 295 GQTYERACIEKWIASGHHTCPTTQQKMSTSALTPNYVLRSLISQWCETNGMEPPKR--ST 354

Query: 121 RNKKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIP 180
           +  K     S  +R+ IDALL KL +   E++RSAA ELRLLAKRN++NRICIAEAGAIP
Sbjct: 355 QPNKPTPACSSSERANIDALLSKLCSPDTEEQRSAAAELRLLAKRNANNRICIAEAGAIP 414

Query: 181 FLVELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAA 240
            L+ LLSS+D RTQEHAVTALLNLSI++ NK +I+   A+P++V VLKNGSMEARENAAA
Sbjct: 415 LLLSLLSSSDLRTQEHAVTALLNLSIHEDNKASIISSGAVPSIVHVLKNGSMEARENAAA 474

Query: 241 TLFSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAG 300
           TLFSLSVIDE KV IG  GAIPAL+ LL EG+ RGKKDAA A+FNL IYQGNK RAIRAG
Sbjct: 475 TLFSLSVIDEYKVTIGGMGAIPALVVLLGEGSQRGKKDAAAALFNLCIYQGNKGRAIRAG 534

Query: 301 IVNPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNREN 360
           +V  +MG + +  G ++DEA+AIL+IL++H EGK AIG AEP+ +L+E I +G+PRNREN
Sbjct: 535 LVPLIMGLVTNPTGALMDEAMAILSILSSHPEGKAAIGAAEPVPVLVEMIGSGTPRNREN 594

Query: 361 AAAVLWSLCSTDFEQLKLAR--EHGAEEALKEVSENGTERAKRKAGSILELFQRF 412
           AAAV+  LCS +   + LAR  E G    L+E++ NGT+R KRKA  +LE   RF
Sbjct: 595 AAAVMLHLCSGEHHLVHLARAQECGIMVPLRELALNGTDRGKRKAVQLLERMSRF 647

BLAST of Cucsat.G10451 vs. ExPASy Swiss-Prot
Match: Q0IMG9 (E3 ubiquitin-protein ligase SPL11 OS=Oryza sativa subsp. japonica OX=39947 GN=SPL11 PE=1 SV=2)

HSP 1 Score: 473.0 bits (1216), Expect = 3.5e-132
Identity = 253/415 (60.96%), Postives = 317/415 (76.39%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKT--TTIKHRSPVIPDDFRCPISLELMRDPVIVST 60
           MS +LKK+KDFVQ++NP++      +   +    R   IPD+FRCPISLELM+DPVIVST
Sbjct: 235 MSMLLKKIKDFVQTQNPDMGPPMASRVLDSNGDSRPITIPDEFRCPISLELMKDPVIVST 294

Query: 61  GQTYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSC 120
           GQTYER+CI+KW+ +GH TCP +QQ +  +ALTPNYVL+SLI+ WCE NG+E PK+  S 
Sbjct: 295 GQTYERACIEKWIASGHHTCPTTQQKMSTSALTPNYVLRSLISQWCETNGMEPPKR--ST 354

Query: 121 RNKKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIP 180
           +  K     S  +R+ IDALL KL +   E++RSAA ELRLLAKRN++NRICIAEAGAIP
Sbjct: 355 QPNKPTPACSSSERANIDALLSKLCSPDTEEQRSAAAELRLLAKRNANNRICIAEAGAIP 414

Query: 181 FLVELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAA 240
            L+ LLSS+D RTQEHAVTALLNLSI++ NK +I+   A+P++V VLKNGSMEARENAAA
Sbjct: 415 LLLSLLSSSDLRTQEHAVTALLNLSIHEDNKASIISSGAVPSIVHVLKNGSMEARENAAA 474

Query: 241 TLFSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAG 300
           TLFSLSVIDE KV IG  GAIPAL+ LL EG+ RGKKDAA A+FNL IYQGNK RAIRAG
Sbjct: 475 TLFSLSVIDEYKVTIGGMGAIPALVVLLGEGSQRGKKDAAAALFNLCIYQGNKGRAIRAG 534

Query: 301 IVNPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNREN 360
           +V  +MG + +  G ++DEA+AIL+IL++H EGK AIG AEP+ +L+E I +G+PRNREN
Sbjct: 535 LVPLIMGLVTNPTGALMDEAMAILSILSSHPEGKAAIGAAEPVPVLVEMIGSGTPRNREN 594

Query: 361 AAAVLWSLCSTDFEQLKLAR--EHGAEEALKEVSENGTERAKRKAGSILELFQRF 412
           AAAV+  LCS +   + LAR  E G    L+E++ NGT+R KRKA  +LE   RF
Sbjct: 595 AAAVMLHLCSGEHHLVHLARAQECGIMVPLRELALNGTDRGKRKAVQLLERMSRF 647

BLAST of Cucsat.G10451 vs. NCBI nr
Match: XP_004142402.1 (U-box domain-containing protein 14 [Cucumis sativus] >KGN52247.1 hypothetical protein Csa_008444 [Cucumis sativus])

HSP 1 Score: 808 bits (2087), Expect = 2.47e-291
Identity = 417/418 (99.76%), Postives = 417/418 (99.76%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           MSSILKKLKDFVQSENPEVE SQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ
Sbjct: 210 MSSILKKLKDFVQSENPEVEISQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 269

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN
Sbjct: 270 TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 329

Query: 121 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 180
           KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL
Sbjct: 330 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 389

Query: 181 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 240
           VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL
Sbjct: 390 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 449

Query: 241 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 300
           FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV
Sbjct: 450 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 509

Query: 301 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 360
           NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA
Sbjct: 510 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 569

Query: 361 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 418
           AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL
Sbjct: 570 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 627

BLAST of Cucsat.G10451 vs. NCBI nr
Match: TYK09254.1 (U-box domain-containing protein 14 [Cucumis melo var. makuwa])

HSP 1 Score: 805 bits (2079), Expect = 4.08e-290
Identity = 414/418 (99.04%), Postives = 417/418 (99.76%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ
Sbjct: 210 MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 269

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN
Sbjct: 270 TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 329

Query: 121 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 180
           KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL
Sbjct: 330 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 389

Query: 181 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 240
           VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVL+NGSMEARENAAATL
Sbjct: 390 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLRNGSMEARENAAATL 449

Query: 241 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 300
           FSLSVIDENKVAIGAAGAIPALI+LLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV
Sbjct: 450 FSLSVIDENKVAIGAAGAIPALISLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 509

Query: 301 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 360
           NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPM ILLEFIRTGSPRNRENAA
Sbjct: 510 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMTILLEFIRTGSPRNRENAA 569

Query: 361 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 418
           AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFD+PSPNL
Sbjct: 570 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDRPSPNL 627

BLAST of Cucsat.G10451 vs. NCBI nr
Match: XP_008446840.1 (PREDICTED: U-box domain-containing protein 14 [Cucumis melo] >KAA0034703.1 U-box domain-containing protein 14 [Cucumis melo var. makuwa])

HSP 1 Score: 803 bits (2074), Expect = 2.35e-289
Identity = 413/418 (98.80%), Postives = 416/418 (99.52%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ
Sbjct: 210 MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 269

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN
Sbjct: 270 TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 329

Query: 121 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 180
           KKAGNNVSDCDRSAIDALLV LVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL
Sbjct: 330 KKAGNNVSDCDRSAIDALLVNLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 389

Query: 181 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 240
           VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVL+NGSMEARENAAATL
Sbjct: 390 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLRNGSMEARENAAATL 449

Query: 241 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 300
           FSLSVIDENKVAIGAAGAIPALI+LLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV
Sbjct: 450 FSLSVIDENKVAIGAAGAIPALISLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 509

Query: 301 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 360
           NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPM ILLEFIRTGSPRNRENAA
Sbjct: 510 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMTILLEFIRTGSPRNRENAA 569

Query: 361 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 418
           AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFD+PSPNL
Sbjct: 570 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDRPSPNL 627

BLAST of Cucsat.G10451 vs. NCBI nr
Match: XP_038891943.1 (U-box domain-containing protein 14 [Benincasa hispida])

HSP 1 Score: 779 bits (2011), Expect = 1.01e-279
Identity = 400/418 (95.69%), Postives = 409/418 (97.85%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           MSS+LKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ
Sbjct: 213 MSSVLKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 272

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERSCIQKWLDAGHKTCPKSQQAL HTALTPNYVLKSLIALWCENNGVELP+KQGSCRN
Sbjct: 273 TYERSCIQKWLDAGHKTCPKSQQALSHTALTPNYVLKSLIALWCENNGVELPRKQGSCRN 332

Query: 121 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 180
           KK GNNVSDCDR+AID LLVKLVNGSPEQKRSAAGELRLLAKRN+DNRICIAEAGAIPFL
Sbjct: 333 KKPGNNVSDCDRAAIDTLLVKLVNGSPEQKRSAAGELRLLAKRNTDNRICIAEAGAIPFL 392

Query: 181 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 240
           VELLSSNDTRTQEHAVTALLNLSINDGNKRTIV+LRAIPA+VEVLKNGSMEARENAAATL
Sbjct: 393 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVNLRAIPAIVEVLKNGSMEARENAAATL 452

Query: 241 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 300
           FSLSVIDENKVAIGAAGAIPALI+LL+EGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV
Sbjct: 453 FSLSVIDENKVAIGAAGAIPALISLLQEGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 512

Query: 301 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 360
           NPLMGFLKDAGGGMVDEALAILAILATHHEGK  IGEA PMAILLEFIRTGSPRNRENAA
Sbjct: 513 NPLMGFLKDAGGGMVDEALAILAILATHHEGKMGIGEAGPMAILLEFIRTGSPRNRENAA 572

Query: 361 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 418
           AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGT+RAKRKAGSILELFQRFD  S NL
Sbjct: 573 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTDRAKRKAGSILELFQRFDGQSANL 630

BLAST of Cucsat.G10451 vs. NCBI nr
Match: XP_022945252.1 (U-box domain-containing protein 14-like [Cucurbita moschata])

HSP 1 Score: 761 bits (1965), Expect = 1.04e-272
Identity = 387/418 (92.58%), Postives = 407/418 (97.37%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           +SSILKKLKD+VQSENPEVETSQDEK+TTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ
Sbjct: 214 LSSILKKLKDYVQSENPEVETSQDEKSTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 273

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERSCIQKWLDAGHKTCPK+QQAL HTALTPNYVLKSLI+LWCENNGVELP+KQG+CR+
Sbjct: 274 TYERSCIQKWLDAGHKTCPKTQQALQHTALTPNYVLKSLISLWCENNGVELPRKQGNCRS 333

Query: 121 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 180
           KK G+++SDCDR+ IDALL+KLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL
Sbjct: 334 KKPGSSISDCDRATIDALLLKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 393

Query: 181 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 240
           VELLSSNDTRTQEHAVTALLNLSIND NKRTIVDLRAIPA+VEVLKNGSMEARENAAATL
Sbjct: 394 VELLSSNDTRTQEHAVTALLNLSINDSNKRTIVDLRAIPAIVEVLKNGSMEARENAAATL 453

Query: 241 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 300
           FSLSVIDENKVAIGAAGAIPALI+LL EGTPRGKKDAATAIFNLSIYQGNKARA+RAGIV
Sbjct: 454 FSLSVIDENKVAIGAAGAIPALISLLCEGTPRGKKDAATAIFNLSIYQGNKARALRAGIV 513

Query: 301 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 360
            PLMGFLKD GGGMVDEALAILAILATHHEGK AIG+AEPMA+LLEFI+TGSPRNRENAA
Sbjct: 514 TPLMGFLKDTGGGMVDEALAILAILATHHEGKVAIGQAEPMAVLLEFIKTGSPRNRENAA 573

Query: 361 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 418
           AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGT+RAKRKAGSILELFQRFD PS  L
Sbjct: 574 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTDRAKRKAGSILELFQRFDGPSSPL 631

BLAST of Cucsat.G10451 vs. ExPASy TrEMBL
Match: A0A0A0KUX4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G622620 PE=4 SV=1)

HSP 1 Score: 808 bits (2087), Expect = 1.20e-291
Identity = 417/418 (99.76%), Postives = 417/418 (99.76%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           MSSILKKLKDFVQSENPEVE SQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ
Sbjct: 210 MSSILKKLKDFVQSENPEVEISQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 269

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN
Sbjct: 270 TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 329

Query: 121 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 180
           KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL
Sbjct: 330 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 389

Query: 181 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 240
           VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL
Sbjct: 390 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 449

Query: 241 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 300
           FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV
Sbjct: 450 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 509

Query: 301 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 360
           NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA
Sbjct: 510 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 569

Query: 361 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 418
           AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL
Sbjct: 570 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 627

BLAST of Cucsat.G10451 vs. ExPASy TrEMBL
Match: A0A5D3CDJ0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002480 PE=4 SV=1)

HSP 1 Score: 805 bits (2079), Expect = 1.98e-290
Identity = 414/418 (99.04%), Postives = 417/418 (99.76%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ
Sbjct: 210 MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 269

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN
Sbjct: 270 TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 329

Query: 121 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 180
           KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL
Sbjct: 330 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 389

Query: 181 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 240
           VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVL+NGSMEARENAAATL
Sbjct: 390 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLRNGSMEARENAAATL 449

Query: 241 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 300
           FSLSVIDENKVAIGAAGAIPALI+LLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV
Sbjct: 450 FSLSVIDENKVAIGAAGAIPALISLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 509

Query: 301 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 360
           NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPM ILLEFIRTGSPRNRENAA
Sbjct: 510 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMTILLEFIRTGSPRNRENAA 569

Query: 361 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 418
           AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFD+PSPNL
Sbjct: 570 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDRPSPNL 627

BLAST of Cucsat.G10451 vs. ExPASy TrEMBL
Match: A0A5A7SW30 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007230 PE=4 SV=1)

HSP 1 Score: 803 bits (2074), Expect = 1.14e-289
Identity = 413/418 (98.80%), Postives = 416/418 (99.52%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ
Sbjct: 210 MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 269

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN
Sbjct: 270 TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 329

Query: 121 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 180
           KKAGNNVSDCDRSAIDALLV LVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL
Sbjct: 330 KKAGNNVSDCDRSAIDALLVNLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 389

Query: 181 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 240
           VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVL+NGSMEARENAAATL
Sbjct: 390 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLRNGSMEARENAAATL 449

Query: 241 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 300
           FSLSVIDENKVAIGAAGAIPALI+LLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV
Sbjct: 450 FSLSVIDENKVAIGAAGAIPALISLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 509

Query: 301 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 360
           NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPM ILLEFIRTGSPRNRENAA
Sbjct: 510 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMTILLEFIRTGSPRNRENAA 569

Query: 361 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 418
           AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFD+PSPNL
Sbjct: 570 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDRPSPNL 627

BLAST of Cucsat.G10451 vs. ExPASy TrEMBL
Match: A0A1S3BG23 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103489445 PE=4 SV=1)

HSP 1 Score: 803 bits (2074), Expect = 1.14e-289
Identity = 413/418 (98.80%), Postives = 416/418 (99.52%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ
Sbjct: 210 MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 269

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN
Sbjct: 270 TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 329

Query: 121 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 180
           KKAGNNVSDCDRSAIDALLV LVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL
Sbjct: 330 KKAGNNVSDCDRSAIDALLVNLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 389

Query: 181 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 240
           VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVL+NGSMEARENAAATL
Sbjct: 390 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLRNGSMEARENAAATL 449

Query: 241 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 300
           FSLSVIDENKVAIGAAGAIPALI+LLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV
Sbjct: 450 FSLSVIDENKVAIGAAGAIPALISLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 509

Query: 301 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 360
           NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPM ILLEFIRTGSPRNRENAA
Sbjct: 510 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMTILLEFIRTGSPRNRENAA 569

Query: 361 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 418
           AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFD+PSPNL
Sbjct: 570 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDRPSPNL 627

BLAST of Cucsat.G10451 vs. ExPASy TrEMBL
Match: A0A6J1G0E6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449552 PE=4 SV=1)

HSP 1 Score: 761 bits (1965), Expect = 5.03e-273
Identity = 387/418 (92.58%), Postives = 407/418 (97.37%), Query Frame = 0

Query: 1   MSSILKKLKDFVQSENPEVETSQDEKTTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 60
           +SSILKKLKD+VQSENPEVETSQDEK+TTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ
Sbjct: 214 LSSILKKLKDYVQSENPEVETSQDEKSTTIKHRSPVIPDDFRCPISLELMRDPVIVSTGQ 273

Query: 61  TYERSCIQKWLDAGHKTCPKSQQALLHTALTPNYVLKSLIALWCENNGVELPKKQGSCRN 120
           TYERSCIQKWLDAGHKTCPK+QQAL HTALTPNYVLKSLI+LWCENNGVELP+KQG+CR+
Sbjct: 274 TYERSCIQKWLDAGHKTCPKTQQALQHTALTPNYVLKSLISLWCENNGVELPRKQGNCRS 333

Query: 121 KKAGNNVSDCDRSAIDALLVKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 180
           KK G+++SDCDR+ IDALL+KLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL
Sbjct: 334 KKPGSSISDCDRATIDALLLKLVNGSPEQKRSAAGELRLLAKRNSDNRICIAEAGAIPFL 393

Query: 181 VELLSSNDTRTQEHAVTALLNLSINDGNKRTIVDLRAIPAVVEVLKNGSMEARENAAATL 240
           VELLSSNDTRTQEHAVTALLNLSIND NKRTIVDLRAIPA+VEVLKNGSMEARENAAATL
Sbjct: 394 VELLSSNDTRTQEHAVTALLNLSINDSNKRTIVDLRAIPAIVEVLKNGSMEARENAAATL 453

Query: 241 FSLSVIDENKVAIGAAGAIPALITLLREGTPRGKKDAATAIFNLSIYQGNKARAIRAGIV 300
           FSLSVIDENKVAIGAAGAIPALI+LL EGTPRGKKDAATAIFNLSIYQGNKARA+RAGIV
Sbjct: 454 FSLSVIDENKVAIGAAGAIPALISLLCEGTPRGKKDAATAIFNLSIYQGNKARALRAGIV 513

Query: 301 NPLMGFLKDAGGGMVDEALAILAILATHHEGKTAIGEAEPMAILLEFIRTGSPRNRENAA 360
            PLMGFLKD GGGMVDEALAILAILATHHEGK AIG+AEPMA+LLEFI+TGSPRNRENAA
Sbjct: 514 TPLMGFLKDTGGGMVDEALAILAILATHHEGKVAIGQAEPMAVLLEFIKTGSPRNRENAA 573

Query: 361 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTERAKRKAGSILELFQRFDKPSPNL 418
           AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGT+RAKRKAGSILELFQRFD PS  L
Sbjct: 574 AVLWSLCSTDFEQLKLAREHGAEEALKEVSENGTDRAKRKAGSILELFQRFDGPSSPL 631

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VZ402.4e-16574.21U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q5VRH91.5e-15167.23U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q9SNC61.5e-13561.48U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1... [more]
A2ZLU63.5e-13260.96Protein spotted leaf 11 OS=Oryza sativa subsp. indica OX=39946 GN=SPL11 PE=1 SV=... [more]
Q0IMG93.5e-13260.96E3 ubiquitin-protein ligase SPL11 OS=Oryza sativa subsp. japonica OX=39947 GN=SP... [more]
Match NameE-valueIdentityDescription
XP_004142402.12.47e-29199.76U-box domain-containing protein 14 [Cucumis sativus] >KGN52247.1 hypothetical pr... [more]
TYK09254.14.08e-29099.04U-box domain-containing protein 14 [Cucumis melo var. makuwa][more]
XP_008446840.12.35e-28998.80PREDICTED: U-box domain-containing protein 14 [Cucumis melo] >KAA0034703.1 U-box... [more]
XP_038891943.11.01e-27995.69U-box domain-containing protein 14 [Benincasa hispida][more]
XP_022945252.11.04e-27292.58U-box domain-containing protein 14-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0KUX41.20e-29199.76RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G622620 PE... [more]
A0A5D3CDJ01.98e-29099.04RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A5A7SW301.14e-28998.80RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3BG231.14e-28998.80RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103489445 PE=4 ... [more]
A0A6J1G0E65.03e-27392.58RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449552... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 455..495
e-value: 0.19
score: 20.8
coord: 496..536
e-value: 380.0
score: 1.0
coord: 537..577
e-value: 120.0
score: 4.8
coord: 414..454
e-value: 0.015
score: 24.5
coord: 373..413
e-value: 3.7E-7
score: 39.8
IPR000225ArmadilloPFAMPF00514Armcoord: 456..494
e-value: 1.3E-6
score: 28.2
coord: 414..453
e-value: 1.3E-4
score: 21.9
coord: 373..412
e-value: 1.1E-10
score: 41.1
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 425..467
score: 8.5924
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 384..426
score: 12.4774
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 466..508
score: 8.6974
IPR003613U box domainSMARTSM00504Ubox_2coord: 249..312
e-value: 1.8E-34
score: 130.5
IPR003613U box domainPFAMPF04564U-boxcoord: 246..316
e-value: 2.3E-20
score: 72.5
IPR003613U box domainPROSITEPS51698U_BOXcoord: 245..319
score: 43.381081
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 244..319
e-value: 5.3E-30
score: 105.1
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 324..418
e-value: 5.9E-17
score: 64.0
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 419..520
e-value: 7.4E-24
score: 86.5
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 521..624
e-value: 2.0E-12
score: 48.4
IPR036537Adaptor protein Cbl, N-terminal domain superfamilyGENE3D1.20.930.20coord: 9..119
e-value: 1.3E-8
score: 36.6
NoneNo IPR availablePANTHERPTHR23315:SF111U-BOX DOMAIN-CONTAINING PROTEIN 14coord: 9..621
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 9..621
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 250..292
e-value: 1.13467E-27
score: 103.082
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 244..321
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 340..617

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G10451.T1Cucsat.G10451.T1mRNA
Cucsat.G10451.T2Cucsat.G10451.T2mRNA
Cucsat.G10451.T4Cucsat.G10451.T4mRNA
Cucsat.G10451.T3Cucsat.G10451.T3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007166 cell surface receptor signaling pathway
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity