Csor.00g313860 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g313860
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionGlycerol-3-phosphate dehydrogenase [NAD(+)]
LocationCsor_Chr15: 1738745 .. 1741198 (-)
RNA-Seq ExpressionCsor.00g313860
SyntenyCsor.00g313860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCTCGGAATCGAAGCTCGACTGCGTTCTAGCAATGGGGGGTGTGTTATGCCGCTCGATGCCCATTTCTCCGATGATGGGGTTTCTCGCAAATCCAAAGTTACCGTCGTTGGAAGTGGCAATTGGGGCAGTGTGGCGGCCAAACTCATCGCCTCTAATGCCGCTAGGCTGAGCTCTTTCCATGGTGTTGTCTTGAACTTCTTCTGAAATCTCATGATTTGTTTGTTATTGTTGCTGGATTTCTATTTTCCCCTTTGCATTTTGAATTATTCATTACTTTTATTGGATTCCAGATGAGGTTAGAATGTGGGTGTATGAGGAGACTCTGCCTACTGGTGAGAAGCTGACTGATGTTATCAACCTCAACAATGTAATGTTCTGTTGATTCATTACGACCCCTTTCCATTTTAGGGAATTTAGAGCGTTACAGATTAGTAATTCTTGGACCTTTATTAACAGGAGAATGTTAAGTATCTCCCTGGTATAAAGCTTGGCATGAATGTTATTGCAGATCCAGACCTAGAAAATGCAGGTCTCATTCTAATCTTTCTGAGTTAACGTGGTGAATCTGCCATGAATTGTGACTCTAATGGGTTGAATCTCAGTGAAGGATGCTAATATGTTGGTGTTTGTGACTCCCCATCAATTTGTGGAGGGGATATGCAAGAGAATGGTTGGAAAGATAAGGAGAGATGTAGAAGCTATTTCACTCATCAAAGGAATGGAGGTGAAGAAGGATGGTCCATGCATGATTTCTTCCCTCATCTCCCAGGAATTGGGTATCAATTGTTGTGTTCTTATGGGGGCGAACATAGCAAACGAGGTTCTAAAACTTGTTGTCCCAATTTTCAGGTCATTTCTTGTTCCGTTTTGTTTTATGATTCTTGAACTGTTCATGCAGATTGCTGTAGAGAAATTCAGTGAAGCAACAGTGGGCTACAGGGAGAACATGAGAGAGATAGCTGAAAAATGGGTTCAACTTTTCAGCACTTCCTACTTTGTTGTTACTCCTGTAAGTACAGTTCATGACCTCATGTTTTATTCAAGCAATCCTTGTTAGCCTTCTAATGGTGAAATATTCAAAGGTCCAAGATGTTGAAGGAGTTGAAATGTGTGGAACTCTTAAGAATGTTGTGGCATTAGCAGCAGGTTAATCATGAACCTTCATCATTTGGCTTCACTTTTGCTGCTTATAATGAGCAACAGCCTTGAAAAATTTGAACATGACAGGTTTTGTTGATGGTTTGGAGATGGGAAATAACACGAAGGTACAAAAATTAGTTCTAAAAGTTCAAAAGGTAACAACCTGAATGTTTATCATATTTTGACTATTTTCACTCAATTGGGTAGGCTGCAATTATGAGAATCGGTTTGAGAGAGATGAGGATGATTTCCAAGCTTCTGTTTTCATCTGTCAAGGACAGTACTTTCTTTGAGAGTTGTGGAGTAGCTGATCTGATTACAACGTGCTGTATGGATAAATTCTTCATTCCTTTTGTTCTTCTTCCATACTCTCTGTCTGTCTTCTTCTTCCATTCTTCCATTCTCTCTGCCTGTCTCACTTAGCCACATCCGTTGGAGAGAGGAACGAAACATTCCTTATTAGGGTGTGAAAACCTCTCTCTAGCAGACACGTTTTAAAACCATGTGGCTGACGGTGATGTGTAACGGGCCAAAATGGACAATATCTGTTAGCGGTAGATTTGGCTTGTTACAGATGGTATTAGAGCCAGCATTGTGTGATGTGCCAGTGAGGACACTGGGTCTCCAAGTGGGGTGGATTATGAGATCCCACATCCGTTGGAGAGGGGAACGAAGTATTCCTTATCAGAGTGTAGAAACCTCTCCCTAGCCGACAGTTTTAAAATCGTGAAGTTGACGAGCGATACGTAACAGGCCAAAACGGACAATATTTGTTAGCGGTGGGTTTGGGCTTGTAACAAATGTGTTTTTGGCAGTGGGAGGAAGAAACAGGAAGGTGGCAGCAGCTTTTGCCAAGAATGAAGGGAAAAGGTTAGTATTGCTACTCAGAAACTTGGATCTTCAACATGGATGCTGCCATGTTTCATCACCGCTGTATATGATTTTTGCCTTCTCCCATTTAGGTCCTTTGATGAACTTGAAGCAGAGATGCTACAGGGCCAAAAATTGCAGGTTTTTGTAATCATTTAGATACTTTTAGAAAGAAAATGTGATAGGACGAACAGGAAAGGAAGATGCTTGTTTGTTTCTTGCTAAAATACTCTCCAGAGCTGATTTTATTACATTTTGAAGGGTGTCTCCACTGCAAAGGAGATGTACGAGGTTTTGAACCACCGTGGGTGGCTGGAGCTGTTCCCACTGTTTGCATCAGTCCACGAGATCTGTATCGGACACCTCTCCCCCTCAGCCATCGTCGAGTATAGCGAGCGCAAACCCAACTTCTGTGTTATTGATGGCCATGCTCAGTAA

mRNA sequence

ATGGCCCTCGGAATCGAAGCTCGACTGCGTTCTAGCAATGGGGGGTGTGTTATGCCGCTCGATGCCCATTTCTCCGATGATGGGGTTTCTCGCAAATCCAAAGTTACCGTCGTTGGAAGTGGCAATTGGGGCAGTGTGGCGGCCAAACTCATCGCCTCTAATGCCGCTAGGCTGAGCTCTTTCCATGATGAGGTTAGAATGTGGGTGTATGAGGAGACTCTGCCTACTGGTGAGAAGCTGACTGATGTTATCAACCTCAACAATGAGAATGTTAAGTATCTCCCTGGTATAAAGCTTGGCATGAATGTTATTGCAGATCCAGACCTAGAAAATGCAGTGAAGGATGCTAATATGTTGGTGTTTGTGACTCCCCATCAATTTGTGGAGGGGATATGCAAGAGAATGGTTGGAAAGATAAGGAGAGATGTAGAAGCTATTTCACTCATCAAAGGAATGGAGGTGAAGAAGGATGGTCCATGCATGATTTCTTCCCTCATCTCCCAGGAATTGGGTATCAATTGTTGTGTTCTTATGGGGGCGAACATAGCAAACGAGATTGCTGTAGAGAAATTCAGTGAAGCAACAGTGGGCTACAGGGAGAACATGAGAGAGATAGCTGAAAAATGGGTTCAACTTTTCAGCACTTCCTACTTTGTTGTTACTCCTGTCCAAGATGTTGAAGGAGTTGAAATGTGTGGAACTCTTAAGAATGTTGTGGCATTAGCAGCAGGTTTTGTTGATGGTTTGGAGATGGGAAATAACACGAAGGCTGCAATTATGAGAATCGGTTTGAGAGAGATGAGGATGATTTCCAAGCTTCTGTTTTCATCTGTCAAGGACAGTACTTTCTTTGAGAGTTGTGGAGTAGCTGATCTGATTACAACGTGCTTGGGAGGAAGAAACAGGAAGGTGGCAGCAGCTTTTGCCAAGAATGAAGGGAAAAGGTCCTTTGATGAACTTGAAGCAGAGATGCTACAGGGCCAAAAATTGCAGGGTGTCTCCACTGCAAAGGAGATGTACGAGGTTTTGAACCACCGTGGGTGGCTGGAGCTGTTCCCACTGTTTGCATCAGTCCACGAGATCTGTATCGGACACCTCTCCCCCTCAGCCATCGTCGAGTATAGCGAGCGCAAACCCAACTTCTGTGTTATTGATGGCCATGCTCAGTAA

Coding sequence (CDS)

ATGGCCCTCGGAATCGAAGCTCGACTGCGTTCTAGCAATGGGGGGTGTGTTATGCCGCTCGATGCCCATTTCTCCGATGATGGGGTTTCTCGCAAATCCAAAGTTACCGTCGTTGGAAGTGGCAATTGGGGCAGTGTGGCGGCCAAACTCATCGCCTCTAATGCCGCTAGGCTGAGCTCTTTCCATGATGAGGTTAGAATGTGGGTGTATGAGGAGACTCTGCCTACTGGTGAGAAGCTGACTGATGTTATCAACCTCAACAATGAGAATGTTAAGTATCTCCCTGGTATAAAGCTTGGCATGAATGTTATTGCAGATCCAGACCTAGAAAATGCAGTGAAGGATGCTAATATGTTGGTGTTTGTGACTCCCCATCAATTTGTGGAGGGGATATGCAAGAGAATGGTTGGAAAGATAAGGAGAGATGTAGAAGCTATTTCACTCATCAAAGGAATGGAGGTGAAGAAGGATGGTCCATGCATGATTTCTTCCCTCATCTCCCAGGAATTGGGTATCAATTGTTGTGTTCTTATGGGGGCGAACATAGCAAACGAGATTGCTGTAGAGAAATTCAGTGAAGCAACAGTGGGCTACAGGGAGAACATGAGAGAGATAGCTGAAAAATGGGTTCAACTTTTCAGCACTTCCTACTTTGTTGTTACTCCTGTCCAAGATGTTGAAGGAGTTGAAATGTGTGGAACTCTTAAGAATGTTGTGGCATTAGCAGCAGGTTTTGTTGATGGTTTGGAGATGGGAAATAACACGAAGGCTGCAATTATGAGAATCGGTTTGAGAGAGATGAGGATGATTTCCAAGCTTCTGTTTTCATCTGTCAAGGACAGTACTTTCTTTGAGAGTTGTGGAGTAGCTGATCTGATTACAACGTGCTTGGGAGGAAGAAACAGGAAGGTGGCAGCAGCTTTTGCCAAGAATGAAGGGAAAAGGTCCTTTGATGAACTTGAAGCAGAGATGCTACAGGGCCAAAAATTGCAGGGTGTCTCCACTGCAAAGGAGATGTACGAGGTTTTGAACCACCGTGGGTGGCTGGAGCTGTTCCCACTGTTTGCATCAGTCCACGAGATCTGTATCGGACACCTCTCCCCCTCAGCCATCGTCGAGTATAGCGAGCGCAAACCCAACTTCTGTGTTATTGATGGCCATGCTCAGTAA

Protein sequence

MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLVFVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHEICIGHLSPSAIVEYSERKPNFCVIDGHAQ
Homology
BLAST of Csor.00g313860 vs. ExPASy Swiss-Prot
Match: Q9SCX9 (Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DHAPRD PE=1 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 1.8e-167
Identity = 298/369 (80.76%), Postives = 326/369 (88.35%), Query Frame = 0

Query: 10  RSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSSFHDEVRMWV 69
           R  NGGC        +DD    KSKVTVVGSGNWGSVAAKLIASNA +L SFHDEVRMWV
Sbjct: 43  RQGNGGC--------NDDS---KSKVTVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWV 102

Query: 70  YEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLVFVTPHQFVE 129
           +EE LP GEKL DVIN  NENVKYLPGIKLG NV+ADPDLENAVKDANMLVFVTPHQF++
Sbjct: 103 FEEVLPNGEKLNDVINKTNENVKYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMD 162

Query: 130 GICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGANIANEIAVE 189
           GICK++ GKI  DVEAISL+KGMEVKK+GPCMISSLIS++LGINCCVLMGANIANEIAVE
Sbjct: 163 GICKKLDGKITGDVEAISLVKGMEVKKEGPCMISSLISKQLGINCCVLMGANIANEIAVE 222

Query: 190 KFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVALAAGFVDGL 249
           KFSEATVGYR   REIA+ WVQLFST YF+VTPV DVEGVE+CGTLKNVVA+AAGFVDGL
Sbjct: 223 KFSEATVGYR-GSREIADTWVQLFSTPYFMVTPVHDVEGVELCGTLKNVVAIAAGFVDGL 282

Query: 250 EMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAFA 309
           EMGNNTKAAIMRIGLREM+ +SKLLF SVKDSTFFESCGVAD+ITTCLGGRNR+VA AFA
Sbjct: 283 EMGNNTKAAIMRIGLREMKALSKLLFPSVKDSTFFESCGVADVITTCLGGRNRRVAEAFA 342

Query: 310 KNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHEICIGHLSPS 369
           K+ GKRSFDELEAEMLQGQKLQGVSTA+E+YEVL H GWLE+FPLF++VH+IC G L P 
Sbjct: 343 KSRGKRSFDELEAEMLQGQKLQGVSTAREVYEVLKHCGWLEMFPLFSTVHQICTGRLQPE 399

Query: 370 AIVEYSERK 379
           AIV+Y E K
Sbjct: 403 AIVQYRENK 399

BLAST of Csor.00g313860 vs. ExPASy Swiss-Prot
Match: P52425 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cuphea lanceolata OX=3930 GN=GPDH PE=2 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 1.5e-166
Identity = 293/350 (83.71%), Postives = 322/350 (92.00%), Query Frame = 0

Query: 32  KSKVTVVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVYEETLPTGEKLTDVINLNNENV 91
           +S+VTVVGSGNWGSVAAKLIA+N  +L SFHDEVRMWV+EETLP+GEKLTDVIN  NENV
Sbjct: 22  RSRVTVVGSGNWGSVAAKLIATNTLKLPSFHDEVRMWVFEETLPSGEKLTDVINQTNENV 81

Query: 92  KYLPGIKLGMNVIADPDLENAVKDANMLVFVTPHQFVEGICKRMVGKIRRDVEAISLIKG 151
           KYLPGIKLG NV+ADPDLENAVKDANMLVFVTPHQF+EGICKR+ GKI+   +A+SLIKG
Sbjct: 82  KYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMEGICKRLEGKIQEGAQALSLIKG 141

Query: 152 MEVKKDGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQ 211
           MEVK +GPCMISSLIS  LGINCCVLMGANIANEIAVEKFSEATVG+REN R+IAEKWVQ
Sbjct: 142 MEVKMEGPCMISSLISDLLGINCCVLMGANIANEIAVEKFSEATVGFREN-RDIAEKWVQ 201

Query: 212 LFSTSYFVVTPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMIS 271
           LFST YF+V+ V+DVEGVE+CGTLKN+VA+AAGFVDGLEMGNNTKAAIMRIGLREM+  S
Sbjct: 202 LFSTPYFMVSAVEDVEGVELCGTLKNIVAIAAGFVDGLEMGNNTKAAIMRIGLREMKAFS 261

Query: 272 KLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAFAKNEGKRSFDELEAEMLQGQKLQ 331
           KLLF SVKD+TFFESCGVADLITTCLGGRNRKVA AFAKN GKRSFD+LEAEML+GQKLQ
Sbjct: 262 KLLFPSVKDTTFFESCGVADLITTCLGGRNRKVAEAFAKNGGKRSFDDLEAEMLRGQKLQ 321

Query: 332 GVSTAKEMYEVLNHRGWLELFPLFASVHEICIGHLSPSAIVEYSERKPNF 382
           GVSTAKE+YEVL HRGWLELFPLF++VHEI  G L PSAIVEYSE+K  F
Sbjct: 322 GVSTAKEVYEVLGHRGWLELFPLFSTVHEISTGRLPPSAIVEYSEQKTIF 370

BLAST of Csor.00g313860 vs. ExPASy Swiss-Prot
Match: O57656 (Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic OS=Takifugu rubripes OX=31033 GN=gpd1 PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 2.1e-91
Identity = 179/335 (53.43%), Postives = 237/335 (70.75%), Query Frame = 0

Query: 34  KVTVVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVYEETLPTGEKLTDVINLNNENVKY 93
           KV ++GSGNWGS  AK++ +NAA+ S F   V+MWV+EET+  G KLT++IN  +ENVKY
Sbjct: 6   KVCIIGSGNWGSAIAKIVGANAAQNSKFDKTVKMWVFEETV-NGRKLTEIINTEHENVKY 65

Query: 94  LPGIKLGMNVIADPDLENAVKDANMLVFVTPHQFVEGICKRMVGKIRRDVEAISLIKGME 153
           LPG KL  NV+A PDL  A KDA++LVFV PHQF+  +C  M G I+++   ISLIKG++
Sbjct: 66  LPGHKLPTNVLAVPDLVEASKDADILVFVIPHQFIGKVCDTMSGNIKKEAIGISLIKGVD 125

Query: 154 VKKDGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQLF 213
              DG  +IS +I ++LGI   VLMGANIANE+A EKF E T+G +        K  +L 
Sbjct: 126 EGPDGLKLISDVIQEKLGITMSVLMGANIANEVADEKFCETTIGCKNKSHGPLLK--ELM 185

Query: 214 STSYFVVTPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKL 273
            T  F VT V++ + VE+CG LKN+VA+ AGF DGL  G+NTKAA++R+GL EM   +++
Sbjct: 186 QTINFRVTVVEEYDVVEICGALKNIVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFARI 245

Query: 274 LFSS--VKDSTFFESCGVADLITTCLGGRNRKVAAAFAKNEGKRSFDELEAEMLQGQKLQ 333
             ++  V  +TF ESCGVADLITTC GGRNRKVA AF K    +S +ELE EML+GQKLQ
Sbjct: 246 FCTAGPVSSATFLESCGVADLITTCYGGRNRKVAKAFVKT--GKSIEELEKEMLEGQKLQ 305

Query: 334 GVSTAKEMYEVLNHRGWLELFPLFASVHEICI-GH 366
           G +TA E+Y +L H+  ++ FPLF +V++IC  GH
Sbjct: 306 GPATAAEVYRILKHKNLIDKFPLFNAVYQICYQGH 335

BLAST of Csor.00g313860 vs. ExPASy Swiss-Prot
Match: Q5EA88 (Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic OS=Bos taurus OX=9913 GN=GPD1 PE=2 SV=3)

HSP 1 Score: 335.1 bits (858), Expect = 1.1e-90
Identity = 174/330 (52.73%), Postives = 232/330 (70.30%), Query Frame = 0

Query: 34  KVTVVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVYEETLPTGEKLTDVINLNNENVKY 93
           KV +VGSGNWGS  AK++  NAA+L+ F   V MWV+EE +  G KLT++IN  +ENVKY
Sbjct: 5   KVCIVGSGNWGSAIAKIVGGNAAQLAHFDPRVTMWVFEEDI-GGRKLTEIINTQHENVKY 64

Query: 94  LPGIKLGMNVIADPDLENAVKDANMLVFVTPHQFVEGICKRMVGKIRRDVEAISLIKGME 153
           LPG KL  NV+A PD+  A  DA++L+FV PHQF+  IC ++ G ++ D   +SLIKG++
Sbjct: 65  LPGHKLPPNVVAVPDVVQAAADADILIFVVPHQFIGKICDQLKGHLKADTIGVSLIKGVD 124

Query: 154 VKKDGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQLF 213
               G  +IS +I + LGI   VLMGANIANE+A EKF E T+G +       +   +L 
Sbjct: 125 EGPKGLKLISEVIGERLGIPMSVLMGANIANEVADEKFCETTIGSKNQAH--GQLLKELM 184

Query: 214 STSYFVVTPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKL 273
            T  F +T VQ+V+ VE+CG LKN+VA+ AGF DGL  G+NTKAA++R+GL EM   +KL
Sbjct: 185 QTPNFRITVVQEVDTVEICGALKNIVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKL 244

Query: 274 LFS-SVKDSTFFESCGVADLITTCLGGRNRKVAAAFAKNEGKRSFDELEAEMLQGQKLQG 333
             S SV  +TF ESCGVADLITTC GGRNRKVA AFA+    +S ++LE EML GQKLQG
Sbjct: 245 FCSGSVSSATFLESCGVADLITTCYGGRNRKVAEAFART--GKSIEQLEKEMLNGQKLQG 304

Query: 334 VSTAKEMYEVLNHRGWLELFPLFASVHEIC 363
             TA+E++ +L H+G ++ FPLF +V+++C
Sbjct: 305 PQTARELHSILQHKGMVDKFPLFTAVYKVC 329

BLAST of Csor.00g313860 vs. ExPASy Swiss-Prot
Match: P21695 (Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic OS=Homo sapiens OX=9606 GN=GPD1 PE=1 SV=4)

HSP 1 Score: 334.0 bits (855), Expect = 2.3e-90
Identity = 173/330 (52.42%), Postives = 236/330 (71.52%), Query Frame = 0

Query: 34  KVTVVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVYEETLPTGEKLTDVINLNNENVKY 93
           KV +VGSGNWGS  AK++  NAA+L+ F   V MWV+EE +  G+KLT++IN  +ENVKY
Sbjct: 5   KVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDI-GGKKLTEIINTQHENVKY 64

Query: 94  LPGIKLGMNVIADPDLENAVKDANMLVFVTPHQFVEGICKRMVGKIRRDVEAISLIKGME 153
           LPG KL  NV+A PD+  A +DA++L+FV PHQF+  IC ++ G ++ +   ISLIKG++
Sbjct: 65  LPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVD 124

Query: 154 VKKDGPCMISSLISQELGINCCVLMGANIANEIAVEKFSEATVGYRENMREIAEKWVQLF 213
              +G  +IS +I + LGI   VLMGANIA+E+A EKF E T+G ++  +   +   +L 
Sbjct: 125 EGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQ--GQLLKELM 184

Query: 214 STSYFVVTPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTKAAIMRIGLREMRMISKL 273
            T  F +T VQ+V+ VE+CG LKNVVA+ AGF DGL  G+NTKAA++R+GL EM   +KL
Sbjct: 185 QTPNFRITVVQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKL 244

Query: 274 LFSS-VKDSTFFESCGVADLITTCLGGRNRKVAAAFAKNEGKRSFDELEAEMLQGQKLQG 333
             S  V  +TF ESCGVADLITTC GGRNRKVA AFA+    +S ++LE E+L GQKLQG
Sbjct: 245 FCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFART--GKSIEQLEKELLNGQKLQG 304

Query: 334 VSTAKEMYEVLNHRGWLELFPLFASVHEIC 363
             TA+E+Y +L H+G ++ FPLF +V+++C
Sbjct: 305 PETARELYSILQHKGLVDKFPLFMAVYKVC 329

BLAST of Csor.00g313860 vs. NCBI nr
Match: KAG6578681.1 (Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 772 bits (1993), Expect = 2.30e-281
Identity = 389/389 (100.00%), Postives = 389/389 (100.00%), Query Frame = 0

Query: 1   MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60
           MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS
Sbjct: 1   MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60

Query: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120
           FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV
Sbjct: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120

Query: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180
           FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA
Sbjct: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180

Query: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240
           NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA
Sbjct: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240

Query: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR
Sbjct: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300

Query: 301 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360
           NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE
Sbjct: 301 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360

Query: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389
           ICIGHLSPSAIVEYSERKPNFCVIDGHAQ
Sbjct: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389

BLAST of Csor.00g313860 vs. NCBI nr
Match: XP_023549523.1 (glycerol-3-phosphate dehydrogenase [NAD(+)] [Cucurbita pepo subsp. pepo])

HSP 1 Score: 755 bits (1950), Expect = 8.26e-275
Identity = 382/389 (98.20%), Postives = 384/389 (98.71%), Query Frame = 0

Query: 1   MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60
           MALGIEARLRSSNG CVMP D+HFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS
Sbjct: 1   MALGIEARLRSSNGVCVMPPDSHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60

Query: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120
           FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV
Sbjct: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120

Query: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180
           FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA
Sbjct: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180

Query: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240
           NIANEIAVEKFSEATVGYRENM EIAE WVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA
Sbjct: 181 NIANEIAVEKFSEATVGYRENMSEIAEIWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240

Query: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR
Sbjct: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300

Query: 301 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360
           NRKVAAA+AKNEGKRSFDELEA MLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE
Sbjct: 301 NRKVAAAYAKNEGKRSFDELEAVMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360

Query: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389
           ICIGHLSPSAIVEYSERKPNFCVIDGHAQ
Sbjct: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389

BLAST of Csor.00g313860 vs. NCBI nr
Match: XP_022992804.1 (LOW QUALITY PROTEIN: glycerol-3-phosphate dehydrogenase [NAD(+)]-like [Cucurbita maxima])

HSP 1 Score: 756 bits (1951), Expect = 3.69e-273
Identity = 381/389 (97.94%), Postives = 383/389 (98.46%), Query Frame = 0

Query: 1   MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60
           M LGIEARLRSSNGGCVMP D+HFSDDGVSRKSKVTVVGS NWGSVAAKLIASNAARLSS
Sbjct: 112 MTLGIEARLRSSNGGCVMPPDSHFSDDGVSRKSKVTVVGSDNWGSVAAKLIASNAARLSS 171

Query: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120
           FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV
Sbjct: 172 FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 231

Query: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180
           FVTPHQFVEGICKRMVGK+RRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA
Sbjct: 232 FVTPHQFVEGICKRMVGKMRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 291

Query: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240
           NIANEIAVEKFSEATVGYRENMRE AEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA
Sbjct: 292 NIANEIAVEKFSEATVGYRENMRERAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 351

Query: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSV DSTFFESCGVADLITTCLGGR
Sbjct: 352 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVNDSTFFESCGVADLITTCLGGR 411

Query: 301 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360
           NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVL HRGWLELFPLFASVHE
Sbjct: 412 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLKHRGWLELFPLFASVHE 471

Query: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389
           ICIGHLSPSAIVEYSERKPNFCVIDGHAQ
Sbjct: 472 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 500

BLAST of Csor.00g313860 vs. NCBI nr
Match: XP_011655380.1 (glycerol-3-phosphate dehydrogenase [NAD(+)] [Cucumis sativus] >XP_011655381.1 glycerol-3-phosphate dehydrogenase [NAD(+)] [Cucumis sativus] >XP_031741351.1 glycerol-3-phosphate dehydrogenase [NAD(+)] [Cucumis sativus] >KAE8648548.1 hypothetical protein Csa_009030 [Cucumis sativus])

HSP 1 Score: 724 bits (1870), Expect = 1.12e-261
Identity = 370/389 (95.12%), Postives = 374/389 (96.14%), Query Frame = 0

Query: 1   MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60
           MA GIEA  RSSNGG V P D+HFSDDGVS K KVTVVGSGNWGSVAAKLIASNAARLSS
Sbjct: 60  MAPGIEALQRSSNGGSVRP-DSHFSDDGVSSKFKVTVVGSGNWGSVAAKLIASNAARLSS 119

Query: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120
           FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLG NVIADPDLENAVKDANMLV
Sbjct: 120 FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLV 179

Query: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180
           FVTPHQFVEGICKR+VGKIR DVEAISLIKGMEVKK+GPCMISSLISQELGINCCVLMGA
Sbjct: 180 FVTPHQFVEGICKRLVGKIRGDVEAISLIKGMEVKKEGPCMISSLISQELGINCCVLMGA 239

Query: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240
           NIANEIAVEKFSEATVGYRENMREIAEKWVQLF+T YFVVTPVQDVEGVEMCGTLKNVVA
Sbjct: 240 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFTTPYFVVTPVQDVEGVEMCGTLKNVVA 299

Query: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR
Sbjct: 300 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 359

Query: 301 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360
           NRKVAAAFA N GKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE
Sbjct: 360 NRKVAAAFAMNGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 419

Query: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389
           IC GHLSPSAIVEYSERKPNF VIDGHAQ
Sbjct: 420 ICTGHLSPSAIVEYSERKPNFSVIDGHAQ 447

BLAST of Csor.00g313860 vs. NCBI nr
Match: KAG6602400.1 (Glycerol-3-phosphate dehydrogenase [NAD(+)], partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 718 bits (1854), Expect = 3.40e-260
Identity = 364/389 (93.57%), Postives = 378/389 (97.17%), Query Frame = 0

Query: 1   MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60
           MA GIEA  RSSNGG V P D+HFSDDGVSRKSKVT+VGSGNWGSVAAKLIASNAARLSS
Sbjct: 1   MAPGIEAPPRSSNGGSVRP-DSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSS 60

Query: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120
           FHDEVRMWV+EETLPTGEKLTDVINLNNENVKYLPGIKLG NVIADPDLENAVKDANMLV
Sbjct: 61  FHDEVRMWVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLV 120

Query: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180
           FVTPHQFVEGICKR+VGKIR++VEAISLIKGME+KK+GPCMISSLISQELGINCCVLMGA
Sbjct: 121 FVTPHQFVEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGA 180

Query: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240
           NIANEIAVEKFSEATVGYRENMREIAEKWVQLFST YFVV+PVQDVEGVEMCGTLKNVVA
Sbjct: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVA 240

Query: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSV+DSTFFESCGVADLITTCLGGR
Sbjct: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVRDSTFFESCGVADLITTCLGGR 300

Query: 301 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360
           NRKVAAAFAK+ GKRSFDELEAEMLQGQKLQGVSTAKEMYEVL+HRGWLELFPLFASVHE
Sbjct: 301 NRKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHE 360

Query: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389
           ICIGHLSPSAIVEYSER+PNF VI+G AQ
Sbjct: 361 ICIGHLSPSAIVEYSERRPNFSVINGQAQ 388

BLAST of Csor.00g313860 vs. ExPASy TrEMBL
Match: A0A6J1JYH7 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC111489027 PE=3 SV=1)

HSP 1 Score: 756 bits (1951), Expect = 1.79e-273
Identity = 381/389 (97.94%), Postives = 383/389 (98.46%), Query Frame = 0

Query: 1   MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60
           M LGIEARLRSSNGGCVMP D+HFSDDGVSRKSKVTVVGS NWGSVAAKLIASNAARLSS
Sbjct: 112 MTLGIEARLRSSNGGCVMPPDSHFSDDGVSRKSKVTVVGSDNWGSVAAKLIASNAARLSS 171

Query: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120
           FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV
Sbjct: 172 FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 231

Query: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180
           FVTPHQFVEGICKRMVGK+RRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA
Sbjct: 232 FVTPHQFVEGICKRMVGKMRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 291

Query: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240
           NIANEIAVEKFSEATVGYRENMRE AEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA
Sbjct: 292 NIANEIAVEKFSEATVGYRENMRERAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 351

Query: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSV DSTFFESCGVADLITTCLGGR
Sbjct: 352 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVNDSTFFESCGVADLITTCLGGR 411

Query: 301 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360
           NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVL HRGWLELFPLFASVHE
Sbjct: 412 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLKHRGWLELFPLFASVHE 471

Query: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389
           ICIGHLSPSAIVEYSERKPNFCVIDGHAQ
Sbjct: 472 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 500

BLAST of Csor.00g313860 vs. ExPASy TrEMBL
Match: A0A5A7T2C3 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold249G00530 PE=3 SV=1)

HSP 1 Score: 718 bits (1853), Expect = 2.34e-260
Identity = 367/389 (94.34%), Postives = 371/389 (95.37%), Query Frame = 0

Query: 1   MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60
           MA GIEA  RSSNGG V P D+H SDDGVS K KVTVVGSGNWGSVAAKLIASNAARLS 
Sbjct: 1   MAPGIEALQRSSNGGSVRP-DSHLSDDGVSSKFKVTVVGSGNWGSVAAKLIASNAARLSC 60

Query: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120
           FHDEVRMWVYEETLPTGEKLTDVIN NNENVKYLPGIKLG NVIADPDLENAVKDANMLV
Sbjct: 61  FHDEVRMWVYEETLPTGEKLTDVINRNNENVKYLPGIKLGKNVIADPDLENAVKDANMLV 120

Query: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180
           FVTPHQFVEGICKR+VGKIR DVEAISLIKGMEVKK+GPCMISSLISQELGINCCVLMGA
Sbjct: 121 FVTPHQFVEGICKRLVGKIRGDVEAISLIKGMEVKKEGPCMISSLISQELGINCCVLMGA 180

Query: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240
           NIANEIAVEKFSEATVGYRENMREIAEKWVQLF+T YFVVTPVQDVEGVEMCGTLKNVVA
Sbjct: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFTTPYFVVTPVQDVEGVEMCGTLKNVVA 240

Query: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR
Sbjct: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300

Query: 301 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360
           NRKVAAAFA N GKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE
Sbjct: 301 NRKVAAAFAMNGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360

Query: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389
           IC GHLSPSAIVEYSERKPNF VIDGHAQ
Sbjct: 361 ICTGHLSPSAIVEYSERKPNFSVIDGHAQ 388

BLAST of Csor.00g313860 vs. ExPASy TrEMBL
Match: A0A6J1JS63 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC111487070 PE=3 SV=1)

HSP 1 Score: 720 bits (1858), Expect = 2.93e-260
Identity = 365/389 (93.83%), Postives = 378/389 (97.17%), Query Frame = 0

Query: 1   MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60
           MA GIEA  RSSNGG V P D+HFSDDGVSRKSKVT+VGSGNWGSVAAKLIASNAARLSS
Sbjct: 54  MAPGIEAPQRSSNGGSVRP-DSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSS 113

Query: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120
           FHDEVRMWV+EETLPTGEKLTDVINLNNENVKYLPGIKLG NVIADPDLENAVKDANMLV
Sbjct: 114 FHDEVRMWVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLV 173

Query: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180
           FVTPHQFVEGICKR+VGKIR++VEAISLIKGME+KK+GPCMISSLISQELGINCCVLMGA
Sbjct: 174 FVTPHQFVEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGA 233

Query: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240
           NIANEIAVEKFSEATVGYRENMREIAEKWVQLFST YFVV+PVQDVEGVEMCGTLKNVVA
Sbjct: 234 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVA 293

Query: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR
Sbjct: 294 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 353

Query: 301 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360
           NRKVAAAFAK+ GKRSFDELEAEMLQGQKLQGVSTAKEMYEVL+HRGWLELFPLFASVHE
Sbjct: 354 NRKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHE 413

Query: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389
           ICIGHLSPSAIVEYSER+PNF VI+G AQ
Sbjct: 414 ICIGHLSPSAIVEYSERRPNFSVINGQAQ 441

BLAST of Csor.00g313860 vs. ExPASy TrEMBL
Match: A0A6J1E2R9 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC111430266 PE=3 SV=1)

HSP 1 Score: 718 bits (1854), Expect = 1.24e-259
Identity = 364/389 (93.57%), Postives = 378/389 (97.17%), Query Frame = 0

Query: 1   MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60
           MA GIEA  RSSNGG V P D+HFSDDGVSRKSKVT+VGSGNWGSVAAKLIASNAARLSS
Sbjct: 55  MAPGIEAPPRSSNGGSVRP-DSHFSDDGVSRKSKVTIVGSGNWGSVAAKLIASNAARLSS 114

Query: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120
           FHDEVRMWV+EETLPTGEKLTDVINLNNENVKYLPGIKLG NVIADPDLENAVKDANMLV
Sbjct: 115 FHDEVRMWVFEETLPTGEKLTDVINLNNENVKYLPGIKLGKNVIADPDLENAVKDANMLV 174

Query: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180
           FVTPHQFVEGICKR+VGKIR++VEAISLIKGME+KK+GPCMISSLISQELGINCCVLMGA
Sbjct: 175 FVTPHQFVEGICKRLVGKIRKEVEAISLIKGMEMKKEGPCMISSLISQELGINCCVLMGA 234

Query: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240
           NIANEIAVEKFSEATVGYRENMREIAEKWVQLFST YFVV+PVQDVEGVEMCGTLKNVVA
Sbjct: 235 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTPYFVVSPVQDVEGVEMCGTLKNVVA 294

Query: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSV+DSTFFESCGVADLITTCLGGR
Sbjct: 295 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVRDSTFFESCGVADLITTCLGGR 354

Query: 301 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360
           NRKVAAAFAK+ GKRSFDELEAEMLQGQKLQGVSTAKEMYEVL+HRGWLELFPLFASVHE
Sbjct: 355 NRKVAAAFAKHGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLSHRGWLELFPLFASVHE 414

Query: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389
           ICIGHLSPSAIVEYSER+PNF VI+G AQ
Sbjct: 415 ICIGHLSPSAIVEYSERRPNFSVINGQAQ 442

BLAST of Csor.00g313860 vs. ExPASy TrEMBL
Match: A0A1S3C9D4 (Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis melo OX=3656 GN=LOC103498126 PE=3 SV=1)

HSP 1 Score: 718 bits (1853), Expect = 2.12e-259
Identity = 367/389 (94.34%), Postives = 371/389 (95.37%), Query Frame = 0

Query: 1   MALGIEARLRSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSS 60
           MA GIEA  RSSNGG V P D+H SDDGVS K KVTVVGSGNWGSVAAKLIASNAARLS 
Sbjct: 60  MAPGIEALQRSSNGGSVRP-DSHLSDDGVSSKFKVTVVGSGNWGSVAAKLIASNAARLSC 119

Query: 61  FHDEVRMWVYEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLV 120
           FHDEVRMWVYEETLPTGEKLTDVIN NNENVKYLPGIKLG NVIADPDLENAVKDANMLV
Sbjct: 120 FHDEVRMWVYEETLPTGEKLTDVINRNNENVKYLPGIKLGKNVIADPDLENAVKDANMLV 179

Query: 121 FVTPHQFVEGICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGA 180
           FVTPHQFVEGICKR+VGKIR DVEAISLIKGMEVKK+GPCMISSLISQELGINCCVLMGA
Sbjct: 180 FVTPHQFVEGICKRLVGKIRGDVEAISLIKGMEVKKEGPCMISSLISQELGINCCVLMGA 239

Query: 181 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVA 240
           NIANEIAVEKFSEATVGYRENMREIAEKWVQLF+T YFVVTPVQDVEGVEMCGTLKNVVA
Sbjct: 240 NIANEIAVEKFSEATVGYRENMREIAEKWVQLFTTPYFVVTPVQDVEGVEMCGTLKNVVA 299

Query: 241 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 300
           LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR
Sbjct: 300 LAAGFVDGLEMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGR 359

Query: 301 NRKVAAAFAKNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 360
           NRKVAAAFA N GKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE
Sbjct: 360 NRKVAAAFAMNGGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHE 419

Query: 361 ICIGHLSPSAIVEYSERKPNFCVIDGHAQ 389
           IC GHLSPSAIVEYSERKPNF VIDGHAQ
Sbjct: 420 ICTGHLSPSAIVEYSERKPNFSVIDGHAQ 447

BLAST of Csor.00g313860 vs. TAIR 10
Match: AT5G40610.1 (NAD-dependent glycerol-3-phosphate dehydrogenase family protein )

HSP 1 Score: 590.1 bits (1520), Expect = 1.3e-168
Identity = 298/369 (80.76%), Postives = 326/369 (88.35%), Query Frame = 0

Query: 10  RSSNGGCVMPLDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSSFHDEVRMWV 69
           R  NGGC        +DD    KSKVTVVGSGNWGSVAAKLIASNA +L SFHDEVRMWV
Sbjct: 43  RQGNGGC--------NDDS---KSKVTVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWV 102

Query: 70  YEETLPTGEKLTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLVFVTPHQFVE 129
           +EE LP GEKL DVIN  NENVKYLPGIKLG NV+ADPDLENAVKDANMLVFVTPHQF++
Sbjct: 103 FEEVLPNGEKLNDVINKTNENVKYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMD 162

Query: 130 GICKRMVGKIRRDVEAISLIKGMEVKKDGPCMISSLISQELGINCCVLMGANIANEIAVE 189
           GICK++ GKI  DVEAISL+KGMEVKK+GPCMISSLIS++LGINCCVLMGANIANEIAVE
Sbjct: 163 GICKKLDGKITGDVEAISLVKGMEVKKEGPCMISSLISKQLGINCCVLMGANIANEIAVE 222

Query: 190 KFSEATVGYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVALAAGFVDGL 249
           KFSEATVGYR   REIA+ WVQLFST YF+VTPV DVEGVE+CGTLKNVVA+AAGFVDGL
Sbjct: 223 KFSEATVGYR-GSREIADTWVQLFSTPYFMVTPVHDVEGVELCGTLKNVVAIAAGFVDGL 282

Query: 250 EMGNNTKAAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLGGRNRKVAAAFA 309
           EMGNNTKAAIMRIGLREM+ +SKLLF SVKDSTFFESCGVAD+ITTCLGGRNR+VA AFA
Sbjct: 283 EMGNNTKAAIMRIGLREMKALSKLLFPSVKDSTFFESCGVADVITTCLGGRNRRVAEAFA 342

Query: 310 KNEGKRSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHEICIGHLSPS 369
           K+ GKRSFDELEAEMLQGQKLQGVSTA+E+YEVL H GWLE+FPLF++VH+IC G L P 
Sbjct: 343 KSRGKRSFDELEAEMLQGQKLQGVSTAREVYEVLKHCGWLEMFPLFSTVHQICTGRLQPE 399

Query: 370 AIVEYSERK 379
           AIV+Y E K
Sbjct: 403 AIVQYRENK 399

BLAST of Csor.00g313860 vs. TAIR 10
Match: AT2G40690.1 (NAD-dependent glycerol-3-phosphate dehydrogenase family protein )

HSP 1 Score: 115.9 bits (289), Expect = 7.1e-26
Identity = 106/358 (29.61%), Postives = 168/358 (46.93%), Query Frame = 0

Query: 20  LDAHFSDDGVSRKSKVTVVGSGNWGSVAAKLIASNAARLSSFHDEVRMWVYEETLPTGEK 79
           L A    D + R  KV V+G G++G+  A  +A     L     EV M V +        
Sbjct: 75  LRAKAKTDVLERTRKVVVLGGGSFGTAMAAHVARRKEGL-----EVNMLVRDSF------ 134

Query: 80  LTDVINLNNENVKYLPGIKLGMNVIADPDLENAVKDANMLVFVTPHQFVEGICKRMVGKI 139
           +   IN N+ N KY P  KL  NVIA  D + A+ DA+  +   P QF     + +   +
Sbjct: 135 VCQSINENHHNCKYFPEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYV 194

Query: 140 RRDVEAISLIKGMEVKKDGPCMISSLISQEL---GINCCVLMGANIANEIAVEKFSEATV 199
              +  ISL KG+E+  +   M+S +I   L         L G + A E+     +   V
Sbjct: 195 DPGLPFISLSKGLEL--NTLRMMSQIIPIALKNPRQPFVALSGPSFALELMNNLPTAMVV 254

Query: 200 GYRENMREIAEKWVQLFSTSYFVVTPVQDVEGVEMCGTLKNVVALAAGFVDGLEMGNNTK 259
             ++  +++A    QL ++SY  +    DV GVE+ G LKNV+A+AAG VDG+ +GNN+ 
Sbjct: 255 ASKD--KKLANAVQQLLASSYLRINTSSDVTGVEIAGALKNVLAIAAGIVDGMNLGNNSM 314

Query: 260 AAIMRIGLREMRMISKLLFSSVKDSTFFESCGVADLITTCLG--GRNRKVAAAFAKNEGK 319
           AA++  G  E+R ++  +    K +T     G  D++ TC     RNR V       E  
Sbjct: 315 AALVSQGCSEIRWLATKM--GAKPTTITGLSGTGDIMLTCFVNLSRNRTVGVRLGSGE-- 374

Query: 320 RSFDELEAEMLQGQKLQGVSTAKEMYEVLNHRGWLELFPLFASVHEICIGHLSPSAIV 373
            + D++   M   Q  +GV+TA  +  +   + +    P+  +V +I    L+P+  V
Sbjct: 375 -TLDDILTSM--NQVAEGVATAGAVIALA--QKYNVKLPVLTAVAKIIDNELTPTKAV 408

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SCX91.8e-16780.76Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic OS=Arabidopsis thal... [more]
P524251.5e-16683.71Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cuphea lanceolata OX=3930 GN=GPDH... [more]
O576562.1e-9153.43Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic OS=Takifugu rubripes OX... [more]
Q5EA881.1e-9052.73Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic OS=Bos taurus OX=9913 G... [more]
P216952.3e-9052.42Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic OS=Homo sapiens OX=9606... [more]
Match NameE-valueIdentityDescription
KAG6578681.12.30e-281100.00Glycerol-3-phosphate dehydrogenase [NAD(+)] 1, chloroplastic, partial [Cucurbita... [more]
XP_023549523.18.26e-27598.20glycerol-3-phosphate dehydrogenase [NAD(+)] [Cucurbita pepo subsp. pepo][more]
XP_022992804.13.69e-27397.94LOW QUALITY PROTEIN: glycerol-3-phosphate dehydrogenase [NAD(+)]-like [Cucurbita... [more]
XP_011655380.11.12e-26195.12glycerol-3-phosphate dehydrogenase [NAD(+)] [Cucumis sativus] >XP_011655381.1 gl... [more]
KAG6602400.13.40e-26093.57Glycerol-3-phosphate dehydrogenase [NAD(+)], partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
A0A6J1JYH71.79e-27397.94Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A5A7T2C32.34e-26094.34Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1JS632.93e-26093.83Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1E2R91.24e-25993.57Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A1S3C9D42.12e-25994.34Glycerol-3-phosphate dehydrogenase [NAD(+)] OS=Cucumis melo OX=3656 GN=LOC103498... [more]
Match NameE-valueIdentityDescription
AT5G40610.11.3e-16880.76NAD-dependent glycerol-3-phosphate dehydrogenase family protein [more]
AT2G40690.17.1e-2629.61NAD-dependent glycerol-3-phosphate dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPRINTSPR00077GPDHDRGNASEcoord: 285..302
score: 75.25
coord: 100..127
score: 47.19
coord: 243..267
score: 61.58
coord: 176..196
score: 55.27
coord: 37..54
score: 54.38
coord: 218..242
score: 51.39
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPIRSFPIRSF000114Glycerol-3-P_dhcoord: 28..380
e-value: 1.6E-86
score: 288.4
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPANTHERPTHR11728GLYCEROL-3-PHOSPHATE DEHYDROGENASEcoord: 30..380
IPR006168Glycerol-3-phosphate dehydrogenase, NAD-dependentPROSITEPS00957NAD_G3PDHcoord: 233..254
IPR011128Glycerol-3-phosphate dehydrogenase, NAD-dependent, N-terminalPFAMPF01210NAD_Gly3P_dh_Ncoord: 34..201
e-value: 2.9E-46
score: 157.3
IPR006109Glycerol-3-phosphate dehydrogenase, NAD-dependent, C-terminalPFAMPF07479NAD_Gly3P_dh_Ccoord: 225..372
e-value: 2.8E-45
score: 154.1
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 226..380
e-value: 2.6E-57
score: 195.5
NoneNo IPR availableGENE3D3.40.50.720coord: 20..223
e-value: 5.2E-73
score: 246.9
NoneNo IPR availablePIRSRPIRSR000114-1PIRSR000114-1coord: 32..376
e-value: 1.4E-97
score: 324.8
NoneNo IPR availablePIRSRPIRSR000114-3PIRSR000114-3coord: 32..376
e-value: 1.4E-97
score: 324.8
NoneNo IPR availablePIRSRPIRSR000114-2PIRSR000114-2coord: 32..376
e-value: 1.4E-97
score: 324.8
NoneNo IPR availablePANTHERPTHR11728:SF39GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]coord: 30..380
IPR017751Glycerol-3-phosphate dehydrogenase, NAD-dependent, eukaryoticTIGRFAMTIGR03376TIGR03376coord: 34..374
e-value: 1.7E-147
score: 489.0
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 224..373
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 32..225

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g313860.m01Csor.00g313860.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0046168 glycerol-3-phosphate catabolic process
biological_process GO:0006116 NADH oxidation
biological_process GO:0006072 glycerol-3-phosphate metabolic process
cellular_component GO:0005829 cytosol
cellular_component GO:0009331 glycerol-3-phosphate dehydrogenase complex
molecular_function GO:0004367 glycerol-3-phosphate dehydrogenase [NAD+] activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor